Rmu_sc0014071.1_g000008

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0014071.1
OG0017172
Physical Location & Seq
Forward (+)
31709 .. 32182
474 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0014071.1_g000008.1.cds

Sequence Viewer

Length: 474 bp
atgaataggaacaaggggaagacgaagaaaagggaaaaggatgaggttgaagagttgctccaagcagcacaggatgacatgctgctcggtctatccgtagactctcacatatcccgcctttctgacgtggaccgccgtttccaggccctcaaactcaagtctccgactccacctaaagttgccgatgctaattccagtgcgcctgctgtcacatcgattcaagtggatgaagagctcaaagcagtcctcggagatgacctgtccaccagattcgcagctctcaaggcctcattgcccatgccctcctcctcctcctcttcttcttcttccatcatcgccgccagctcggcattgcacgctgactacgacgacgacgacgctgttgatgaagatgatgaagttgaaaagttgatacgttgggccaaggacgccgctcgccttgacccttctccaccctccgacgatgaagaatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

157

Amino Acids

17.0

Weight (kDa)

4.53

Isoelectric Point (pI)

56.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017172)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G24370
fragaria_vesca FvH4_4g37240
malus_domestica MD13G1002400.v1.1
prunus_persica Prupe.1G350000_v2.0.a1
rosa_chinensis RchiOBHm_Chr4g0447031
rosa_laevigata RLG00000005611
rosa_multiflora Rmu_sc0014071.1_g000008 Rmu_sc0019356.1_g000003
rosa_roxburghii Rroxscaffold_5G00387350
rosa_rugosa Rorug04G0377200
rosa_samantha Rh4BG447700 Rh4CG464800
rosa_wichuraiana Rw4G037520

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 434
AccI GTMKAC 1 cut(s) 99
AciI CCGC 4 cut(s) 115, 133, 339, 432
AcyI GRCGYC 1 cut(s) 429
AfiI CCNNNNNNNGG 1 cut(s) 142
AgsI TTSAA 3 cut(s) 50, 221, 404
AjiI CACGTC 1 cut(s) 127
AjnI CCWGG 1 cut(s) 141
AluBI AGCT 3 cut(s) 235, 278, 345
AluI AGCT 3 cut(s) 235, 278, 345
Alw21I GWGCWC 1 cut(s) 237
Alw26I GTCTC 1 cut(s) 165
AoxI GGCC 3 cut(s) 144, 285, 420
ApeKI GCWGC 3 cut(s) 65, 82, 275
AspLEI GCGC 1 cut(s) 202
AspS9I GGNCC 3 cut(s) 130, 145, 420
AvaII GGWCC 1 cut(s) 130
BanII GRGCYC 1 cut(s) 237
BbsI GAAGAC 1 cut(s) 26
Bbv12I GWGCWC 1 cut(s) 237
BbvI GCAGC 3 cut(s) 69, 77, 287
BccI CCATC 1 cut(s) 338
BceAI ACGGC 1 cut(s) 120
BciT130I CCWGG 1 cut(s) 143
BcoDI GTCTC 1 cut(s) 165
BglI GCCNNNNNGGC 1 cut(s) 347
BisI GCNGC 5 cut(s) 66, 83, 276, 339, 432
BlsI GCNGC 5 cut(s) 67, 84, 277, 340, 433
Bme1390I CCNGG 1 cut(s) 143
Bme18I GGWCC 1 cut(s) 130
BmgBI CACGTC 1 cut(s) 127
BmgT120I GGNCC 3 cut(s) 130, 145, 420
BmrFI CCNGG 1 cut(s) 143
BmsI GCATC 1 cut(s) 175
BpiI GAAGAC 1 cut(s) 26
BpuEI CTTGAG 2 cut(s) 140, 266
Bsa29I ATCGAT 1 cut(s) 215
BsaHI GRCGYC 1 cut(s) 429
BsaJI CCNNGG 2 cut(s) 247, 423
Bsc4I CCNNNNNNNGG 1 cut(s) 142
Bse1I ACTGG 1 cut(s) 195
Bse3DI GCAATG 2 cut(s) 290, 350
BseBI CCWGG 1 cut(s) 143
BseCI ATCGAT 1 cut(s) 215
BseDI CCNNGG 2 cut(s) 247, 423
BseGI GGATG 3 cut(s) 46, 79, 232
BseLI CCNNNNNNNGG 1 cut(s) 142
BseMI GCAATG 2 cut(s) 290, 350
BseNI ACTGG 1 cut(s) 195
BseRI GAGGAG 4 cut(s) 295, 298, 301, 304
BseXI GCAGC 3 cut(s) 69, 77, 287
BshFI GGCC 3 cut(s) 146, 287, 422
BshVI ATCGAT 1 cut(s) 215
BsiHKAI GWGCWC 1 cut(s) 237
BslI CCNNNNNNNGG 1 cut(s) 142
BsmAI GTCTC 1 cut(s) 165
BsnI GGCC 3 cut(s) 146, 287, 422
Bsp1286I GDGCHC 1 cut(s) 237
BspACI CCGC 4 cut(s) 115, 133, 339, 432
BspANI GGCC 3 cut(s) 146, 287, 422
BspDI ATCGAT 1 cut(s) 215
BspQI GCTCTTC 1 cut(s) 225
BsrBI CCGCTC 1 cut(s) 434
BsrDI GCAATG 2 cut(s) 290, 350
BsrI ACTGG 1 cut(s) 195
BssECI CCNNGG 2 cut(s) 247, 423
BssNI GRCGYC 1 cut(s) 429
BssT1I CCWWGG 1 cut(s) 423
Bst2UI CCWGG 1 cut(s) 143
Bst6I CTCTTC 3 cut(s) 45, 225, 322
BstACI GRCGYC 1 cut(s) 429
BstC8I GCNNGC 4 cut(s) 204, 343, 357, 436
BstF5I GGATG 3 cut(s) 46, 79, 232
BstHHI GCGC 1 cut(s) 202
BstMAI GTCTC 1 cut(s) 165
BstMWI GCNNNNNNNGC 4 cut(s) 284, 347, 356, 428
BstNI CCWGG 1 cut(s) 143
BstNSI RCATGY 1 cut(s) 82
BstSCI CCNGG 1 cut(s) 141
BstV1I GCAGC 3 cut(s) 69, 77, 287
BstV2I GAAGAC 1 cut(s) 26
Bsu15I ATCGAT 1 cut(s) 215
BsuRI GGCC 3 cut(s) 146, 287, 422
BsuTUI ATCGAT 1 cut(s) 215
BtgZI GCGATG 1 cut(s) 319
BtrI CACGTC 1 cut(s) 127
BtsCI GGATG 3 cut(s) 46, 79, 232
BtsIMutI CAGTG 1 cut(s) 202
Cac8I GCNNGC 4 cut(s) 204, 343, 357, 436
CfoI GCGC 1 cut(s) 202
Cfr13I GGNCC 3 cut(s) 130, 145, 420
ClaI ATCGAT 1 cut(s) 215
CseI GACGC 2 cut(s) 386, 437
CviAII CATG 2 cut(s) 79, 298
CviJI RGCY 6 cut(s) 146, 235, 278, 287, 345, 422
CviKI_1 RGCY 6 cut(s) 146, 235, 278, 287, 345, 422
Eam1104I CTCTTC 3 cut(s) 45, 225, 322
EarI CTCTTC 3 cut(s) 45, 225, 322
Ecl136II GAGCTC 1 cut(s) 235
Eco130I CCWWGG 1 cut(s) 423
Eco147I AGGCCT 1 cut(s) 287
Eco24I GRGCYC 1 cut(s) 237
Eco47I GGWCC 1 cut(s) 130
Eco53kI GAGCTC 1 cut(s) 235
EcoICRI GAGCTC 1 cut(s) 235
EcoO109I RGGNCCY 1 cut(s) 145
EcoRII CCWGG 1 cut(s) 141
EcoT14I CCWWGG 1 cut(s) 423
EcoT38I GRGCYC 1 cut(s) 237
ErhI CCWWGG 1 cut(s) 423
FaeI CATG 2 cut(s) 82, 301
FaiI YATR 3 cut(s) 80, 110, 299
FatI CATG 2 cut(s) 78, 297
FauI CCCGC 1 cut(s) 122
FblI GTMKAC 1 cut(s) 99
Fnu4HI GCNGC 5 cut(s) 66, 83, 276, 339, 432
FokI GGATG 3 cut(s) 53, 86, 239
FriOI GRGCYC 1 cut(s) 237
Fsp4HI GCNGC 5 cut(s) 66, 83, 276, 339, 432
GlaI GCGC 1 cut(s) 201
GluI GCNGC 5 cut(s) 66, 83, 276, 339, 432
HaeIII GGCC 3 cut(s) 146, 287, 422
HgaI GACGC 2 cut(s) 386, 437
HhaI GCGC 1 cut(s) 202
Hin1I GRCGYC 1 cut(s) 429
Hin1II CATG 2 cut(s) 82, 301
Hin6I GCGC 1 cut(s) 200
HinP1I GCGC 1 cut(s) 200
HinfI GANTC 4 cut(s) 101, 166, 217, 270
Hpy166II GTNNAC 3 cut(s) 100, 130, 264
Hpy188I TCNGA 4 cut(s) 124, 165, 251, 460
Hpy8I GTNNAC 3 cut(s) 100, 130, 264
Hpy99I CGWCG 5 cut(s) 371, 374, 377, 380, 464
HpyAV CCTTC 1 cut(s) 456
HpyCH4IV ACGT 2 cut(s) 126, 415
HpyCH4V TGCA 1 cut(s) 355
HpyF10VI GCNNNNNNNGC 4 cut(s) 284, 347, 356, 428
HpySE526I ACGT 2 cut(s) 126, 415
Hsp92I GRCGYC 1 cut(s) 429
Hsp92II CATG 2 cut(s) 82, 301
HspAI GCGC 1 cut(s) 200
LguI GCTCTTC 1 cut(s) 225
LmnI GCTCC 1 cut(s) 63
LpnPI CCDG 8 cut(s) 56, 128, 155, 208, 216, 272, 280, 355
Lsp1109I GCAGC 3 cut(s) 69, 77, 287
LweI GCATC 1 cut(s) 175
MaeII ACGT 2 cut(s) 126, 415
MaeIII GTNAC 1 cut(s) 208
MbiI CCGCTC 1 cut(s) 434
MboII GAAGA 9 cut(s) 31, 37, 62, 242, 309, 312, 315, 318, 401
MhlI GDGCHC 1 cut(s) 237
MluCI AATT 1 cut(s) 190
MlyI GAGTC 2 cut(s) 95, 160
MmeI TCCRAC 1 cut(s) 188
MspR9I CCNGG 1 cut(s) 143
MvaI CCWGG 1 cut(s) 143
MwoI GCNNNNNNNGC 4 cut(s) 284, 347, 356, 428
NlaIII CATG 2 cut(s) 82, 301
NmeAIII GCCGAG 1 cut(s) 326
NmuCI GTSAC 1 cut(s) 208
NspI RCATGY 1 cut(s) 82
PceI AGGCCT 1 cut(s) 287
PciSI GCTCTTC 1 cut(s) 225
PcsI WCGNNNNNNNCGW 3 cut(s) 93, 363, 372
PfeI GAWTC 2 cut(s) 217, 270
PkrI GCNGC 5 cut(s) 67, 84, 277, 340, 433
PleI GAGTC 2 cut(s) 95, 160
PpsI GAGTC 2 cut(s) 95, 160
Psp124BI GAGCTC 1 cut(s) 237
Psp6I CCWGG 1 cut(s) 141
PspGI CCWGG 1 cut(s) 141
PspPI GGNCC 3 cut(s) 130, 145, 420
SacI GAGCTC 1 cut(s) 237
SapI GCTCTTC 1 cut(s) 225
SatI GCNGC 5 cut(s) 66, 83, 276, 339, 432
Sau96I GGNCC 3 cut(s) 130, 145, 420
SchI GAGTC 2 cut(s) 95, 160
ScrFI CCNGG 1 cut(s) 143
SduI GDGCHC 1 cut(s) 237
SetI ASST 8 cut(s) 48, 129, 175, 237, 261, 280, 347, 418
SfaNI GCATC 1 cut(s) 175
SinI GGWCC 1 cut(s) 130
SmlI CTYRAG 2 cut(s) 155, 281
SmoI CTYRAG 2 cut(s) 155, 281
Sse9I AATT 1 cut(s) 190
SseBI AGGCCT 1 cut(s) 287
SsiI CCGC 4 cut(s) 115, 133, 339, 432
SstI GAGCTC 1 cut(s) 237
StuI AGGCCT 1 cut(s) 287
StyD4I CCNGG 1 cut(s) 141
StyI CCWWGG 1 cut(s) 423
TaiI ACGT 2 cut(s) 129, 418
TaqI TCGA 1 cut(s) 215
TaqII GACCGA 1 cut(s) 77
TasI AATT 1 cut(s) 190
TauI GCSGC 2 cut(s) 341, 434
TfiI GAWTC 2 cut(s) 217, 270
TscAI CASTG 1 cut(s) 202
TseFI GTSAC 1 cut(s) 208
TseI GCWGC 3 cut(s) 65, 82, 275
Tsp45I GTSAC 1 cut(s) 208
TspDTI ATGAA 4 cut(s) 17, 243, 402, 411
TspGWI ACGGA 1 cut(s) 85
TspRI CASTG 1 cut(s) 202
VpaK11BI GGWCC 1 cut(s) 130
XceI RCATGY 1 cut(s) 82
XmiI GTMKAC 1 cut(s) 99
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.