Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0004469

Physical Location & Seq

Genomic Coordinates Reverse (-)

1735099 .. 1742807

Gene Structure 7709 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1024500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2865 bp

ATGGACGAGGACGTCGTACAGCGAGTCTTCCAAGAAGGAGGCCGCGACTACTTCCAGCAGCAACCCTCCACTTCCTCTTCCTCTTCCTCAATTCTCCAATCGCTTCCTCTCCATGTGTCTTTCGATCACGGATATTATTTGCTAGTAAAGTCTATCCAAGAACTTAGAGAGAAAAAAGAGGGCATTGTAACAGTTGGCATTGGTGGTCCAAGTGGTTCTGGTAAAACAAGCTTAGCAGAAAAGGTGGCATCTGTTATTGGTTGCACTGTTGTGTCAATGGAGAACTATCGTGATGGATTTGATGAAGGGAATGATCTGGGTTCAATAGATTTTGATATGCTGGTCAGAAATCTTGAGGATTTGACAAAGGGTGAGGATACATTGATTCCAGTATTTGACTATCAACAAAAGAAACGTGTTGGTTCAAAAACAATAAAGAGTGCTTCATCTGGGGTGGTAATAGTTGATGGTACCTATGCTTTGCATGCAAAGCTTCGCTCTTTGCTAGATATTCGAGTTGCAGTGGTTGGTGGTGTTCATTTTAGCCTTCTATCCAAAGTTCGATATGATATCGGAGATTCTTGTTCATTGGATTATCTTATTGACAGCATTTTCCCATTGTTCAGGAAGCACATTGAACCAGACCTTCATCATGCACAGATTAGGATTAACAACAGCTTTGTCTCATCATTTAGGGAGGCAATCTACAAGCTGAAATGCAGGAGTGAGGCAGCAGAGGGGCATTTTGCTTATGCCTTCCAAGGAGGTGAACCTCAAATAGATAATTTTATTGAGATGTATCTTAGGCCTCCTTCAGCGAGTGAAGAAGCACGGATAAATGATTGGATCAAGGTGCGTCAATCGGGTATAAGATATTATCTCTCACTGGGGGACCAGAGGATTGTTGACAAAAATTTCATTATCAGACCAAAAGCTGAGTTTGAGGTTGGAAGGATGACCCTGGGTGGGTTACTGGCTTTGGGATATGTTGTGGTTGTTAGTTACAAACGAGCATCAAAATCAGTTGATAATGGCAATGCTTCCTTGTCACTTGAAACCATTGATTCCCTTGGTGAGACCTTCATGGTGCTGAGGGGCACAAATAGAAAAACAGTTGGAACTGAAGCATTGAAAATGGGTATCACTGAGCCTTGGATCACTAAATCATATCTAGAAATGATTCTTGAGAGCAAAGGTGTACCTCGCCTTAATACACCACCCCTTTTGCCAAATACATCTATGGCCAGTGATCAGGACAAAACAATTGTTGCACCAAGACCAATTCGCGTTCCTCCAAACCTTGTTACTCGGCTTGAGGATCTATCTCAGCCATGGACTAGATCTCCAACAAAATCTAAGATGGAACCTATATTAGCAACATGGCATTTCATCTCATCTGATCCTTCCCAAGCCGAAAACTCAACCATAGATCCATCCTCTTTCAGGGATACCATGCAACTTGCTCCAATGCCTGACTCGTATGACTTGGATCGAGGATTGCTTCTTGCTGTGCAAGCAATACAGGCATTGTTGGAGAATAAAGGGTTTCCAGTTATAGTTGGAATTGGAGGTCCAAGTGGTTCTGGAAAAACTAGTTTGGCTCATAAAATGGCAAACATAGTAGGATGTGAAGTTATTTCGCTTGAAAGCTATTATAAATCTGAACAAGTTAAGGATTTCAAGTATGATGACTTCAGCTCGCTGGATATGTCTTTGCTTTCAAAAAACATTGATGATATAAGGAATGGTCAAAGGACAAAAGTACCTTTATTTGATTTGGAAACTGGTGCTCGGAGTGACTTTAAGGAACTTGAAGTTTCTGAAGACTGTGGAGTGATTATTTTTGAAGGAGTTTATGCTTTGCATCCACATATCCGAAAATCACTTGACTTGTGGATTGCTGTTGTTGGAGGTGTTCATTCACACCTCATTTCTAGAGTTCAAAGGGATAAAAGTAGAGTGGGGTGTTTTATGTCCCAGAATGAGATCATGATGACAGTGTTTCCAATGTTCCAGCAGTTCATTGAACCCCATCTTGTTCATGCACATCTCAAAATTCGAAATGACTTTGATCCAGTGCTTTCCCCTGAGAGCTCATTGTTTGTATTGAAGAGTAACAAGCAAGTGGCATACCAAGATATTTTAAAAATACTGGATCCTTCAAAGTTCTGCAGCTCCGTGCAGAATTTCATTGATATATACTTGAAGCTTCCTGGACTTCCCACTAATGGGCAGTTAACAGAGGGTGACTGCATTAGGGTCAGAATATGTGAGGGCAGATTTGCATTGCTGATACGGGAGCCTATAAGAGAAGGAAACTTCATCATCCAACCTAAAGTGGATTTTGATATTAGCATTAGCACAGTTGCGGGGCTTCTTAACCTTGGGTATCAAGCCGTAGCTTATATTGAAGCATCTGCATTTATCTACCAAGATGGAAAGATTTTGGTTGAGGTCGATCATCTACAAGATGCCCCAAACCCTTACTTGCAAATCAAGGGGGTTGATAAAGATGCTGTAGCAGCTGCAGGGTCAACGCTTAAACTGGATGATTCATATACTACGAAGAGCTATCTTCAAATAGTTCTGGAAAGATTGCCAGCATTGGGAAGAGGCTCAGGTGGAATTCACGCTCAGCAAGCTGCAAGGTTGCAGGAACTTGTGGAATTTATTCAATCTCAGGGCAGCAGTTCAGCTTCTGAATCCTCACCAATCAGGGAAGTTTCTCCGGTTGAAGGAGTTATCGAAGACATCCAGTCAAGGATTAGAAGACTTGAGCGGTGGCATACAATCAATACGGTACTATGGACATTTTTAATGTCTGCGCTCGTTGGTTACTCACTCTACGAAAGGAAGCGTCAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004849 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006206 GO:0006213 GO:0006220 GO:0006221 GO:0006222 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008655 GO:0009058 GO:0009112 GO:0009116 GO:0009117 GO:0009119 GO:0009123 GO:0009124 GO:0009129 GO:0009130 GO:0009156 GO:0009161 GO:0009163 GO:0009165 GO:0009173 GO:0009174 GO:0009218 GO:0009220 GO:0009259 GO:0009260 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0018130 GO:0019205 GO:0019206 GO:0019438 GO:0019637 GO:0019693 GO:0034404 GO:0034641 GO:0034654 GO:0042455 GO:0043094 GO:0043097 GO:0043174 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046049 GO:0046131 GO:0046132 GO:0046134 GO:0046390 GO:0046483 GO:0055086 GO:0071704 GO:0072527 GO:0072528 GO:0090407 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659

KEGG Pathways

Metabolic & Signaling

ko00240 ko00983 ko01100 map00240 map00983 map01100

Pfam Domains

Protein Families

Cytidylate_kin PRK

Protein Analysis

955

Amino Acids

106.22

Weight (kDa)

5.93

Isoelectric Point (pI)

44.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PRK	PF00485	108 - 223	1.7e-06	Phosphoribulokinase / Uridine kinase family
PRK	PF00485	519 - 686	9.3e-17	Phosphoribulokinase / Uridine kinase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004469)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G01460 AT2G01460 AT2G01460
fragaria_vesca	FvH4_4g32570 FvH4_4g32570 FvH4_4g32570 FvH4_4g32570 FvH4_4g32570 FvH4_4g32570 FvH4_4g32570 FvH4_4g32570 FvH4_4g32570 FvH4_4g32570
malus_domestica	MD13G1024500.v1.1 MD16G1027200.v1.1
prunus_persica	Prupe.1G326000_v2.0.a1 Prupe.1G326000_v2.0.a1 Prupe.1G326000_v2.0.a1 Prupe.1G326000_v2.0.a1 Prupe.1G326000_v2.0.a1 Prupe.1G326000_v2.0.a1
pyrus_communis	pycom16g02300
rosa_chinensis	RchiOBHm_Chr4g0441441
rosa_laevigata	RLG00000006092
rosa_multiflora	Rmu_sc0003360.1_g000006
rosa_roxburghii	Rroxscaffold_5G00382310
rosa_rugosa	Rorug04G0333700
rosa_samantha	Rh4AG385200 Rh4BG399400 Rh4CG414400 Rh4DG392000
rosa_wichuraiana	Rw4G033270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1656
AasI	GACNNNNNNGTC	1 cut(s)	11
AatII	GACGTC	1 cut(s)	15
Acc65I	GGTACC	1 cut(s)	470
AccB1I	GGYRCC	1 cut(s)	470
AccBSI	CCGCTC	1 cut(s)	2779
AccII	CGCG	2 cut(s)	45, 1287
AciI	CCGC	3 cut(s)	43, 2369, 2779
AclWI	GGATC	9 cut(s)	854, 1163, 1326, 1394, 1424, 1497, 2066, 2150, 2163
AcoI	YGGCCR	1 cut(s)	1242
AcsI	RAATTY	5 cut(s)	913, 2055, 2185, 2625, 2666
AcuI	CTGAAG	4 cut(s)	798, 1143, 1678, 1842
AcyI	GRCGYC	1 cut(s)	12
AfaI	GTAC	5 cut(s)	18, 472, 1200, 1764, 2802
AfiI	CCNNNNNNNGG	6 cut(s)	966, 1443, 2228, 2229, 2606, 2735
AflIII	ACRYGT	1 cut(s)	415
AhdI	GACNNNNNGTC	1 cut(s)	2755
AhlI	ACTAGT	1 cut(s)	1592
AjnI	CCWGG	2 cut(s)	960, 2212
AleI	CACNNNNGTG	1 cut(s)	269
Alw21I	GWGCWC	2 cut(s)	1792, 2096
Alw26I	GTCTC	2 cut(s)	688, 1070
AlwI	GGATC	9 cut(s)	854, 1163, 1326, 1394, 1424, 1497, 2066, 2150, 2163
AlwNI	CAGNNNCTG	1 cut(s)	2699
AoxI	GGCC	3 cut(s)	40, 806, 1242
ApeKI	GCWGC	7 cut(s)	58, 731, 2172, 2522, 2525, 2642, 2685
ApoI	RAATTY	5 cut(s)	913, 2055, 2185, 2625, 2666
Asp700I	GAANNNNTTC	2 cut(s)	1179, 2670
Asp718I	GGTACC	1 cut(s)	470
AspLEI	GCGC	1 cut(s)	2827
AspS9I	GGNCC	3 cut(s)	206, 892, 1571
AsuHPI	GGTGA	5 cut(s)	383, 779, 1085, 2258, 2700
AsuII	TTCGAA	1 cut(s)	2059
AvaII	GGWCC	3 cut(s)	206, 892, 1571
BaeGI	GKGCMC	1 cut(s)	1100
BaeI	ACNNNNGTAYC	2 cut(s)	1746, 1779
BalI	TGGCCA	1 cut(s)	1244
BamHI	GGATCC	1 cut(s)	2155
BanI	GGYRCC	1 cut(s)	470
BanII	GRGCYC	1 cut(s)	2096
BarI	GAAGNNNNNNTAC	2 cut(s)	1676, 1708
BbsI	GAAGAC	4 cut(s)	19, 1830, 2754, 2776
Bbv12I	GWGCWC	2 cut(s)	1792, 2096
BbvCI	CCTCAGC	1 cut(s)	1091
BbvI	GCAGC	7 cut(s)	70, 743, 2184, 2512, 2534, 2629, 2697
BccI	CCATC	6 cut(s)	287, 461, 1354, 1441, 2040, 2429
BceAI	ACGGC	1 cut(s)	2381
BciT130I	CCWGG	2 cut(s)	962, 2214
BciVI	GTATCC	2 cut(s)	370, 1441
BclI	TGATCA	1 cut(s)	1249
BcoDI	GTCTC	2 cut(s)	688, 1070
BcuI	ACTAGT	1 cut(s)	1592
BfaI	CTAG	6 cut(s)	143, 506, 1172, 1338, 1593, 1935
BfmI	CTRYAG	3 cut(s)	2170, 2517, 2526
BfuI	GTATCC	2 cut(s)	370, 1441
BglII	AGATCT	1 cut(s)	1340
BisI	GCNGC	8 cut(s)	43, 59, 732, 2173, 2523, 2526, 2643, 2686
BlpI	GCTNAGC	2 cut(s)	232, 2634
BlsI	GCNGC	8 cut(s)	44, 60, 733, 2174, 2524, 2527, 2644, 2687
Bme1390I	CCNGG	2 cut(s)	962, 2214
Bme18I	GGWCC	3 cut(s)	206, 892, 1571
BmeRI	GACNNNNNGTC	1 cut(s)	2755
BmgT120I	GGNCC	3 cut(s)	206, 892, 1571
BmiI	GGNNCC	5 cut(s)	472, 893, 1365, 2157, 2301
BmrFI	CCNGG	2 cut(s)	962, 2214
BmrI	ACTGGG	1 cut(s)	896
BmsI	GCATC	6 cut(s)	257, 1022, 1873, 2423, 2461, 2503
BmuI	ACTGGG	1 cut(s)	896
BpiI	GAAGAC	4 cut(s)	19, 1830, 2754, 2776
Bpu10I	CCTNAGC	2 cut(s)	1091, 2617
Bpu1102I	GCTNAGC	2 cut(s)	232, 2634
Bpu14I	TTCGAA	1 cut(s)	2059
BpuEI	CTTGAG	4 cut(s)	374, 1205, 1334, 2795
BsaHI	GRCGYC	1 cut(s)	12
BsaI	GGTCTC	1 cut(s)	1070
BsaJI	CCNNGG	7 cut(s)	760, 960, 961, 1069, 1151, 1331, 2383
BsaWI	WCCGGW	1 cut(s)	2728
BsaXI	ACNNNNNCTCC	2 cut(s)	1327, 1357
Bsc4I	CCNNNNNNNGG	6 cut(s)	966, 1443, 2228, 2229, 2606, 2735
Bse3DI	GCAATG	2 cut(s)	1042, 2285
BseBI	CCWGG	2 cut(s)	962, 2214
BseDI	CCNNGG	7 cut(s)	760, 960, 961, 1069, 1151, 1331, 2383
BseGI	GGATG	7 cut(s)	960, 1433, 1631, 1864, 2325, 2554, 2751
BseLI	CCNNNNNNNGG	6 cut(s)	966, 1443, 2228, 2229, 2606, 2735
BseMI	GCAATG	2 cut(s)	1042, 2285
BseMII	CTCAG	8 cut(s)	927, 1082, 1137, 1340, 2079, 2631, 2648, 2693
BseSI	GKGCMC	1 cut(s)	1100
BseXI	GCAGC	7 cut(s)	70, 743, 2184, 2512, 2534, 2629, 2697
BsgI	GTGCAG	1 cut(s)	2201
Bsh1236I	CGCG	2 cut(s)	45, 1287
BshFI	GGCC	3 cut(s)	42, 808, 1244
BshNI	GGYRCC	1 cut(s)	470
BsiHKAI	GWGCWC	2 cut(s)	1792, 2096
BsiSI	CCGG	1 cut(s)	2729
BslFI	GGGAC	2 cut(s)	905, 1960
BslI	CCNNNNNNNGG	6 cut(s)	966, 1443, 2228, 2229, 2606, 2735
BsmAI	GTCTC	2 cut(s)	688, 1070
BsmFI	GGGAC	2 cut(s)	905, 1960
BsnI	GGCC	3 cut(s)	42, 808, 1244
Bso31I	GGTCTC	1 cut(s)	1070
Bsp119I	TTCGAA	1 cut(s)	2059
Bsp1286I	GDGCHC	3 cut(s)	1100, 1792, 2096
Bsp1720I	GCTNAGC	2 cut(s)	232, 2634
Bsp19I	CCATGG	1 cut(s)	1331
BspACI	CCGC	3 cut(s)	43, 2369, 2779
BspANI	GGCC	3 cut(s)	42, 808, 1244
BspCNI	CTCAG	8 cut(s)	928, 1083, 1138, 1339, 2080, 2630, 2647, 2692
BspFNI	CGCG	2 cut(s)	45, 1287
BspHI	TCATGA	1 cut(s)	1989
BspLI	GGNNCC	5 cut(s)	472, 893, 1365, 2157, 2301
BspMAI	CTGCAG	2 cut(s)	2174, 2530
BspPI	GGATC	9 cut(s)	854, 1163, 1326, 1394, 1424, 1497, 2066, 2150, 2163
BspQI	GCTCTTC	1 cut(s)	2561
BspT104I	TTCGAA	1 cut(s)	2059
BspT107I	GGYRCC	1 cut(s)	470
BspTNI	GGTCTC	1 cut(s)	1070
BsrBI	CCGCTC	1 cut(s)	2779
BsrDI	GCAATG	2 cut(s)	1042, 2285
BssECI	CCNNGG	7 cut(s)	760, 960, 961, 1069, 1151, 1331, 2383
BssNI	GRCGYC	1 cut(s)	12
BssT1I	CCWWGG	5 cut(s)	760, 1069, 1151, 1331, 2383
Bst2UI	CCWGG	2 cut(s)	962, 2214
Bst4CI	ACNGT	7 cut(s)	193, 268, 1114, 1829, 1999, 2365, 2800
Bst6I	CTCTTC	5 cut(s)	82, 88, 2105, 2561, 2605
BstACI	GRCGYC	1 cut(s)	12
BstBI	TTCGAA	1 cut(s)	2059
BstC8I	GCNNGC	5 cut(s)	486, 1515, 1700, 2601, 2640
BstDSI	CCRYGG	1 cut(s)	1331
BstENI	CCTNNNNNAGG	1 cut(s)	1441
BstF5I	GGATG	7 cut(s)	960, 1433, 1631, 1864, 2325, 2554, 2751
BstFNI	CGCG	2 cut(s)	45, 1287
BstHHI	GCGC	1 cut(s)	2827
BstMAI	GTCTC	2 cut(s)	688, 1070
BstMWI	GCNNNNNNNGC	6 cut(s)	485, 490, 1514, 1523, 2522, 2639
BstNI	CCWGG	2 cut(s)	962, 2214
BstNSI	RCATGY	1 cut(s)	488
BstSCI	CCNGG	2 cut(s)	960, 2212
BstSFI	CTRYAG	3 cut(s)	2170, 2517, 2526
BstSLI	GKGCMC	1 cut(s)	1100
BstUI	CGCG	2 cut(s)	45, 1287
BstV1I	GCAGC	7 cut(s)	70, 743, 2184, 2512, 2534, 2629, 2697
BstV2I	GAAGAC	4 cut(s)	19, 1830, 2754, 2776
BstX2I	RGATCY	4 cut(s)	1318, 1340, 1429, 2155
BstYI	RGATCY	4 cut(s)	1318, 1340, 1429, 2155
BsuI	GTATCC	2 cut(s)	370, 1441
BsuRI	GGCC	3 cut(s)	42, 808, 1244
BtgI	CCRYGG	1 cut(s)	1331
BtsCI	GGATG	7 cut(s)	960, 1433, 1631, 1864, 2325, 2554, 2751
BtsI	GCAGTG	1 cut(s)	528
BtsIMutI	CAGTG	7 cut(s)	264, 528, 884, 1143, 1252, 2004, 2082
Cac8I	GCNNGC	5 cut(s)	486, 1515, 1700, 2601, 2640
CaiI	CAGNNNCTG	1 cut(s)	2699
CciI	TCATGA	1 cut(s)	1989
CfoI	GCGC	1 cut(s)	2827
Cfr13I	GGNCC	3 cut(s)	206, 892, 1571
CseI	GACGC	2 cut(s)	845, 2846
Csp6I	GTAC	5 cut(s)	17, 471, 1199, 1763, 2801
CspCI	CAANNNNNGTGG	2 cut(s)	1206, 1241
CviAII	CATG	9 cut(s)	113, 485, 653, 1084, 1332, 1380, 1453, 1990, 2042
CviQI	GTAC	5 cut(s)	17, 471, 1199, 1763, 2801
DraI	TTTAAA	1 cut(s)	2145
DrdI	GACNNNNNNGTC	1 cut(s)	11
DriI	GACNNNNNGTC	1 cut(s)	2755
DseDI	GACNNNNNNGTC	1 cut(s)	11
EaeI	YGGCCR	1 cut(s)	1242
Eam1104I	CTCTTC	5 cut(s)	82, 88, 2105, 2561, 2605
Eam1105I	GACNNNNNGTC	1 cut(s)	2755
EarI	CTCTTC	5 cut(s)	82, 88, 2105, 2561, 2605
Ecl136II	GAGCTC	1 cut(s)	2094
Eco130I	CCWWGG	5 cut(s)	760, 1069, 1151, 1331, 2383
Eco147I	AGGCCT	1 cut(s)	808
Eco24I	GRGCYC	1 cut(s)	2096
Eco31I	GGTCTC	1 cut(s)	1070
Eco32I	GATATC	1 cut(s)	571
Eco47I	GGWCC	3 cut(s)	206, 892, 1571
Eco53kI	GAGCTC	1 cut(s)	2094
Eco57I	CTGAAG	4 cut(s)	798, 1143, 1678, 1842
EcoICRI	GAGCTC	1 cut(s)	2094
EcoNI	CCTNNNNNAGG	1 cut(s)	1441
EcoRI	GAATTC	1 cut(s)	2625
EcoRII	CCWGG	2 cut(s)	960, 2212
EcoRV	GATATC	1 cut(s)	571
EcoT14I	CCWWGG	5 cut(s)	760, 1069, 1151, 1331, 2383
EcoT38I	GRGCYC	1 cut(s)	2096
ErhI	CCWWGG	5 cut(s)	760, 1069, 1151, 1331, 2383
FaeI	CATG	9 cut(s)	116, 488, 656, 1087, 1335, 1383, 1456, 1993, 2045
FaqI	GGGAC	2 cut(s)	905, 1960
FatI	CATG	9 cut(s)	112, 484, 652, 1083, 1331, 1379, 1452, 1989, 2041
FauI	CCCGC	1 cut(s)	2362
FbaI	TGATCA	1 cut(s)	1249
Fnu4HI	GCNGC	8 cut(s)	43, 59, 732, 2173, 2523, 2526, 2643, 2686
FokI	GGATG	7 cut(s)	967, 1420, 1638, 1851, 2312, 2561, 2738
FriOI	GRGCYC	1 cut(s)	2096
Fsp4HI	GCNGC	8 cut(s)	43, 59, 732, 2173, 2523, 2526, 2643, 2686
FspBI	CTAG	6 cut(s)	143, 506, 1172, 1338, 1593, 1935
GlaI	GCGC	1 cut(s)	2826
GluI	GCNGC	8 cut(s)	43, 59, 732, 2173, 2523, 2526, 2643, 2686
HaeIII	GGCC	3 cut(s)	42, 808, 1244
HapII	CCGG	1 cut(s)	2729
HgaI	GACGC	2 cut(s)	845, 2846
HhaI	GCGC	1 cut(s)	2827
Hin1I	GRCGYC	1 cut(s)	12
Hin1II	CATG	9 cut(s)	116, 488, 656, 1087, 1335, 1383, 1456, 1993, 2045
Hin6I	GCGC	1 cut(s)	2825
HinP1I	GCGC	1 cut(s)	2825
HincII	GTYRAC	3 cut(s)	907, 2238, 2535
HindII	GTYRAC	3 cut(s)	907, 2238, 2535
HindIII	AAGCTT	3 cut(s)	229, 491, 2207
HinfI	GANTC	8 cut(s)	24, 385, 578, 1064, 1180, 1475, 2552, 2702
HpaI	GTTAAC	1 cut(s)	2238
HpaII	CCGG	1 cut(s)	2729
HphI	GGTGA	5 cut(s)	383, 779, 1085, 2258, 2700
Hpy166II	GTNNAC	5 cut(s)	770, 907, 1199, 2238, 2535
Hpy8I	GTNNAC	5 cut(s)	770, 907, 1199, 2238, 2535
Hpy99I	CGWCG	1 cut(s)	17
HpyCH4III	ACNGT	7 cut(s)	193, 268, 1114, 1829, 1999, 2365, 2800
HpyCH4IV	ACGT	2 cut(s)	12, 415
HpyF10VI	GCNNNNNNNGC	6 cut(s)	485, 490, 1514, 1523, 2522, 2639
HpySE526I	ACGT	2 cut(s)	12, 415
Hsp92I	GRCGYC	1 cut(s)	12
Hsp92II	CATG	9 cut(s)	116, 488, 656, 1087, 1335, 1383, 1456, 1993, 2045
HspAI	GCGC	1 cut(s)	2825
KpnI	GGTACC	1 cut(s)	474
Ksp22I	TGATCA	1 cut(s)	1249
KspAI	GTTAAC	1 cut(s)	2238
LguI	GCTCTTC	1 cut(s)	2561
LmnI	GCTCC	3 cut(s)	1468, 2180, 2299
Lsp1109I	GCAGC	7 cut(s)	70, 743, 2184, 2512, 2534, 2629, 2697
LweI	GCATC	6 cut(s)	257, 1022, 1873, 2423, 2461, 2503
MaeI	CTAG	6 cut(s)	143, 506, 1172, 1338, 1593, 1935
MaeII	ACGT	2 cut(s)	12, 415
MaeIII	GTNAC	9 cut(s)	187, 969, 1001, 1047, 1303, 1796, 2114, 2246, 2834
MbiI	CCGCTC	1 cut(s)	2779
MfeI	CAATTG	1 cut(s)	1263
MflI	RGATCY	4 cut(s)	1318, 1340, 1429, 2155
MhlI	GDGCHC	3 cut(s)	1100, 1792, 2096
MlsI	TGGCCA	1 cut(s)	1244
MluNI	TGGCCA	1 cut(s)	1244
MlyI	GAGTC	2 cut(s)	33, 1469
MmeI	TCCRAC	7 cut(s)	928, 1096, 1370, 1512, 1540, 1888, 2353
Mox20I	TGGCCA	1 cut(s)	1244
MroXI	GAANNNNTTC	2 cut(s)	1179, 2670
MscI	TGGCCA	1 cut(s)	1244
MseI	TTAA	9 cut(s)	669, 1209, 1671, 1803, 2144, 2237, 2379, 2541, 2816
MslI	CAYNNNNRTG	1 cut(s)	269
Msp20I	TGGCCA	1 cut(s)	1244
MspA1I	CMGCKG	1 cut(s)	2525
MspI	CCGG	1 cut(s)	2729
MspR9I	CCNGG	2 cut(s)	962, 2214
MunI	CAATTG	1 cut(s)	1263
MvaI	CCWGG	2 cut(s)	962, 2214
MvnI	CGCG	2 cut(s)	45, 1287
MwoI	GCNNNNNNNGC	6 cut(s)	485, 490, 1514, 1523, 2522, 2639
NcoI	CCATGG	1 cut(s)	1331
NlaIII	CATG	9 cut(s)	116, 488, 656, 1087, 1335, 1383, 1456, 1993, 2045
NlaIV	GGNNCC	5 cut(s)	472, 893, 1365, 2157, 2301
NmeAIII	GCCGAG	1 cut(s)	1288
NmuCI	GTSAC	3 cut(s)	1047, 1796, 2246
NspI	RCATGY	1 cut(s)	488
NspV	TTCGAA	1 cut(s)	2059
OliI	CACNNNNGTG	1 cut(s)	269
PaeI	GCATGC	1 cut(s)	488
PagI	TCATGA	1 cut(s)	1989
PasI	CCCWGGG	1 cut(s)	961
PceI	AGGCCT	1 cut(s)	808
PciSI	GCTCTTC	1 cut(s)	2561
PcsI	WCGNNNNNNNCGW	1 cut(s)	12
PdmI	GAANNNNTTC	2 cut(s)	1179, 2670
PfeI	GAWTC	6 cut(s)	385, 578, 1064, 1180, 2552, 2702
PfoI	TCCNGGA	1 cut(s)	2212
PkrI	GCNGC	8 cut(s)	44, 60, 733, 2174, 2524, 2527, 2644, 2687
PleI	GAGTC	2 cut(s)	32, 1469
PpsI	GAGTC	2 cut(s)	32, 1469
PsiI	TTATAA	1 cut(s)	1656
Psp124BI	GAGCTC	1 cut(s)	2096
Psp6I	CCWGG	2 cut(s)	960, 2212
PspGI	CCWGG	2 cut(s)	960, 2212
PspN4I	GGNNCC	5 cut(s)	472, 893, 1365, 2157, 2301
PspPI	GGNCC	3 cut(s)	206, 892, 1571
PstI	CTGCAG	2 cut(s)	2174, 2530
PstNI	CAGNNNCTG	1 cut(s)	2699
PsuI	RGATCY	4 cut(s)	1318, 1340, 1429, 2155
PvuII	CAGCTG	1 cut(s)	2525
RsaI	GTAC	5 cut(s)	18, 472, 1200, 1764, 2802
RsaNI	GTAC	5 cut(s)	17, 471, 1199, 1763, 2801
RseI	CAYNNNNRTG	1 cut(s)	269
SacI	GAGCTC	1 cut(s)	2096
SapI	GCTCTTC	1 cut(s)	2561
SaqAI	TTAA	9 cut(s)	669, 1209, 1671, 1803, 2144, 2237, 2379, 2541, 2816
SatI	GCNGC	8 cut(s)	43, 59, 732, 2173, 2523, 2526, 2643, 2686
Sau96I	GGNCC	3 cut(s)	206, 892, 1571
SchI	GAGTC	2 cut(s)	33, 1469
ScrFI	CCNGG	2 cut(s)	962, 2214
SduI	GDGCHC	3 cut(s)	1100, 1792, 2096
SfaNI	GCATC	6 cut(s)	257, 1022, 1873, 2423, 2461, 2503
SfcI	CTRYAG	3 cut(s)	2170, 2517, 2526
SfuI	TTCGAA	1 cut(s)	2059
SinI	GGWCC	3 cut(s)	206, 892, 1571
SmiMI	CAYNNNNRTG	1 cut(s)	269
SmlI	CTYRAG	4 cut(s)	353, 1184, 1313, 2774
SmoI	CTYRAG	4 cut(s)	353, 1184, 1313, 2774
SpeI	ACTAGT	1 cut(s)	1592
SphI	GCATGC	1 cut(s)	488
SseBI	AGGCCT	1 cut(s)	808
SsiI	CCGC	3 cut(s)	43, 2369, 2779
SspMI	CTAG	6 cut(s)	143, 506, 1172, 1338, 1593, 1935
SstI	GAGCTC	1 cut(s)	2096
StuI	AGGCCT	1 cut(s)	808
StyD4I	CCNGG	2 cut(s)	960, 2212
StyI	CCWWGG	5 cut(s)	760, 1069, 1151, 1331, 2383
TaaI	ACNGT	7 cut(s)	193, 268, 1114, 1829, 1999, 2365, 2800
TaiI	ACGT	2 cut(s)	15, 418
TaqI	TCGA	7 cut(s)	123, 514, 562, 1492, 2059, 2457, 2745
TauI	GCSGC	1 cut(s)	45
TfiI	GAWTC	6 cut(s)	385, 578, 1064, 1180, 2552, 2702
Tru1I	TTAA	9 cut(s)	669, 1209, 1671, 1803, 2144, 2237, 2379, 2541, 2816
Tru9I	TTAA	9 cut(s)	669, 1209, 1671, 1803, 2144, 2237, 2379, 2541, 2816
TscAI	CASTG	7 cut(s)	271, 528, 891, 1150, 1252, 2004, 2082
TseFI	GTSAC	3 cut(s)	1047, 1796, 2246
TseI	GCWGC	7 cut(s)	58, 731, 2172, 2522, 2525, 2642, 2685
Tsp45I	GTSAC	3 cut(s)	1047, 1796, 2246
TspGWI	ACGGA	3 cut(s)	144, 847, 2167
TspRI	CASTG	7 cut(s)	271, 528, 891, 1150, 1252, 2004, 2082
VpaK11BI	GGWCC	3 cut(s)	206, 892, 1571
XagI	CCTNNNNNAGG	1 cut(s)	1441
XapI	RAATTY	5 cut(s)	913, 2055, 2185, 2625, 2666
XbaI	TCTAGA	2 cut(s)	1171, 1934
XceI	RCATGY	1 cut(s)	488
XmnI	GAANNNNTTC	2 cut(s)	1179, 2670
XspI	CTAG	6 cut(s)	143, 506, 1172, 1338, 1593, 1935
ZraI	GACGTC	1 cut(s)	13

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.