Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0004469

Physical Location & Seq

Genomic Coordinates Reverse (-)

1914117 .. 1922992

Gene Structure 8876 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1027200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3120 bp

ATGGACGACGACGTCGTCAAGCGAGTCTTCCAAGAAGGAGGCCGCGACTATTTCCAGCAGCAACCCTCCACTTCCTCCTCCTCAATTCTCCAATCGCTTCCTCTCCATGTGTCTTTCGATCACGGATATTATTTGCTAGTAAAGTCTATCCAAGAACTTAGAGAGAAAAAAGAGGGTCTTGTAACAGTTGGCATTGGTGGTCCAAGTGGTTCTGGTAAAACAAGCTTAGCAGAAAAGGTGGCATCTGTTATTGGTTGCACTGTTGTATCAATGGAGAACTATCGTGATGGATTTGATGAAGGGAATGATCTGGGTTCAATAGATTTTGATATGCTTGTCAGAAATCTCGAGGATTTGACAAAGGGCGAGGATACATTGATTCCAGTATTTGACTATCAACAAAAGAAACGTGTTGGTTCAAAAACAATAAAGAGTGCTTCATCTGGGGTGGTAATAGTTGATGGTACCTATGCTTTGCATGCAAAGCTTCGCTCTTTGCTAGATATTCGAGTTGCAGTGGTTGGTGGTGTTCATTTTAGCCTTCTGTCCAAAGTTCGATATGATATCGGAGATTCTTGTTCACTGGATTACCTTATTGACAGCATTTTCCCATTGTTCAGGAAGCACATTGAACCAGACCTTCATCATGCACAGATTAGGATTAACAACAGCTTTGTCTCATCATTTAGGGAGGCAATCTACAAGCTGAAATGCCGGAGTGAGGCAGCAGAGGGGCATCTTGCTTATGCTTTTCAAGGAAGTGAAGCTCAAATAGATAATTTTATTGAGATGTATCTTAGGCCTCCTTCGGCAAGTGAAGAAGCGCGGATAAATGATTGGATCAAGGTGCGTCAATCGGGTATAAGATATTATCTGTCACTGGGGGACCAGAGGATTGTTGACAAAAATTTCATTATCAGGCCAAAAGCTGAGTTTGAGGTAAGAAGTATTATCATAAGTTTCAATTATAGTATTGACACTAGAGAATACACAAAGCCACTGATCATCGGGATATACTTTAGGTTGGTTCCTCATCTATCTACATTCTGTTTTAGGGTTTATTGTTCTTATGGAAGCCTTCTGGTCAGTGACGAGCAAATAATGGGGTTATTCTTTGGGGATTTTAGATGTATATTTATGTGTGAAATTTTTGTGAAGGCCTGCTCCTTGTGTAATAGTACTGTAGAGTTTCTAGATGTTGGAAGGATGACTCTGGGCGGGTTACTGGCTTTGGGATATGCTGTGGTTGTTAGTTACAAACGAGCATCAAAATCAGTCGATAATGGCAATGTTTCCTTGTCACTTGAAACCATTGATACCCTTGGTGAGACATTCATGGTGCTGAGGGGCACAGATAGAAAAACAGTTGGAACTGAAGCATTGAAGATGGGTATCACTGAGCCTTGGATCACTAAATCATATCTTGAAATGATTCTTGAGAGCAAAGAAGGTGTACCTCGCCTTAATACACCACCCCTTTTGTCAAATACATCTATGACCAGTAATCAGGACAAAACGATTGTTGCACCAAGACCAATTCGTGTTCCTCCAAACCTTGTTACTCGGCTTGAGGATCTATCTCAGCCATGGACTAGATCTCCAACAAAATCTAAGATGGAGCCTATATTAGCGACATGGCATTTCATCTCATCTGATCCTTCCCAAGCTGAAAGCTCAACCATAGATCCTTCCTCTTTCAGGGATACCATGCAACTTGCTCCAATGCCTGATTCGTATGACTTGGATCGAGGATTGCTTCTTGCTGTGCAAGCAATACAGGCATTGTTGGAGAATAAAGGGTTTCCAGTTATAGTTGGAATTGGAGGTCCAAGTGGTTCTGGGAAAACTAGTTTGGCTCATAAAATGGCAAACATAGTAGGATGTGAAGTTGTTTCGCTTGAAAGCTATTATAAATCTGAACAAGTTAAGGATTTCAAGTATGATGACTTCAGCTCGCTCGATCTGTCTTTGCTTTCAAAAAACATTGATGACATAAGGAATGGTCAAAGGACAAAAGTACCCGTATTTGATTTGGAAACTGGTGCTCGGAGTTGCTTTAAGGAACTTGAAGTTTCTGAAGACTGTGGAGTGATTATTTTTGAAGGAGTTTATGCTTTGCATCCAAATATCCGAAAATCACTTGACTTGTGGATTGCTGTTGTTGGAGGTGTTCATTCACACCTCATTTCTAGAGTTCAAAGGGATAAAAGTAGAGTGGGGTGTTTTATGTCCCAGAATGAGATCATGATGACGGTGTTTCCAATGTTCCAGCAGTTCATTGAACCCCATCTTGTTCATGCACACCTCAAAATTCGAAATGACTTTGATCCAGTGCTTTCCCCTGAGAGCTCATCATTTGTATTGAAGAGTAACAAGCAAGTGGCATATCAAGATATTTTAAAAATACTGGATCCTGCGAAGTTCTGCAGCTCTGTGCAGAATTTCATTGATATATACTTGAAGCTTCCTGGACTTCCTACTAATGGTCAGTTAACAGAGGGTGACTGCATTAGGGTCAGAATATGTGAGGGCAGATTTGCATTGCTGATACGGGAGCCTATCAGAGAAGGAAACTTCATCATCCAACCGAAAGTGGATTTCGATATTAGCATTAGTACAGTTGCTGGGCTTCTTAACCTTGGGTATCAAGCTGTAGCTTATATTGAAGCATCTGCGTTCATCTACCAAGACACAAAGATTTTAGTTGAGGTCGATCATCTACAAGATTCCCCAAACCCTTACGTGCAAATCAAGGGGGTTGATAAAGATGCTGTAGCAGATTCAGGATCAATGCTTAAACTGGATGGTTCATATACTACAAAGAGCTATCTTCAAATAGTTCTGGAAAGATTGCCAGCGTTGGGAAGAGGCTCGGGTGGAATTCACGCTCAGCAAGCCGCAAGGTTGCAGGAACTTGTTGAATTTATTCAATCTCAGGGTAGCAGTTCAGCTTCTGAATCGTCGCCGATCAGGGAAGTTTCTCCGGTTGAAGGAGTTATCGAAGACATGCAGTCAAGGATTAGAAGACTTGAGCGGTGGCATACAATCAATACGGTACTCTGGACATTTTTAATGTCTGCGCTCGTTGGTTATTCGCTCTACCAAAGGAAGCGTCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004849 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006139 GO:0006206 GO:0006213 GO:0006220 GO:0006221 GO:0006222 GO:0006725 GO:0006753 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0008655 GO:0009058 GO:0009112 GO:0009116 GO:0009117 GO:0009119 GO:0009123 GO:0009124 GO:0009129 GO:0009130 GO:0009156 GO:0009161 GO:0009163 GO:0009165 GO:0009173 GO:0009174 GO:0009218 GO:0009220 GO:0009259 GO:0009260 GO:0009987 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0018130 GO:0019205 GO:0019206 GO:0019438 GO:0019637 GO:0019693 GO:0034404 GO:0034641 GO:0034654 GO:0042455 GO:0043094 GO:0043097 GO:0043174 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046049 GO:0046131 GO:0046132 GO:0046134 GO:0046390 GO:0046483 GO:0055086 GO:0071704 GO:0072527 GO:0072528 GO:0090407 GO:1901135 GO:1901137 GO:1901293 GO:1901360 GO:1901362 GO:1901564 GO:1901566 GO:1901576 GO:1901657 GO:1901659

KEGG Pathways

Metabolic & Signaling

ko00240 ko00983 ko01100 map00240 map00983 map01100

Pfam Domains

Protein Families

Cytidylate_kin PRK

Protein Analysis

1040

Amino Acids

116.1

Weight (kDa)

6.0

Isoelectric Point (pI)

42.35

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PRK	PF00485	106 - 221	1.7e-06	Phosphoribulokinase / Uridine kinase family
PRK	PF00485	604 - 771	1.2e-17	Phosphoribulokinase / Uridine kinase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Publication-ready

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Orthologous Genes (Group: OG0004469)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G01460 AT2G01460 AT2G01460
fragaria_vesca	FvH4_4g32570 FvH4_4g32570 FvH4_4g32570 FvH4_4g32570 FvH4_4g32570 FvH4_4g32570 FvH4_4g32570 FvH4_4g32570 FvH4_4g32570 FvH4_4g32570
malus_domestica	MD13G1024500.v1.1 MD16G1027200.v1.1
prunus_persica	Prupe.1G326000_v2.0.a1 Prupe.1G326000_v2.0.a1 Prupe.1G326000_v2.0.a1 Prupe.1G326000_v2.0.a1 Prupe.1G326000_v2.0.a1 Prupe.1G326000_v2.0.a1
pyrus_communis	pycom16g02300
rosa_chinensis	RchiOBHm_Chr4g0441441
rosa_laevigata	RLG00000006092
rosa_multiflora	Rmu_sc0003360.1_g000006
rosa_roxburghii	Rroxscaffold_5G00382310
rosa_rugosa	Rorug04G0333700
rosa_samantha	Rh4AG385200 Rh4BG399400 Rh4CG414400 Rh4DG392000
rosa_wichuraiana	Rw4G033270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1911
AasI	GACNNNNNNGTC	2 cut(s)	11, 14
AatII	GACGTC	1 cut(s)	15
Acc65I	GGTACC	1 cut(s)	464
AccB1I	GGYRCC	1 cut(s)	464
AccBSI	CCGCTC	1 cut(s)	3034
AccII	CGCG	2 cut(s)	45, 826
AciI	CCGC	5 cut(s)	43, 826, 1218, 2898, 3034
AcsI	RAATTY	6 cut(s)	907, 1146, 2310, 2440, 2880, 2921
AcuI	CTGAAG	3 cut(s)	1395, 1933, 2097
AcyI	GRCGYC	1 cut(s)	12
AfaI	GTAC	6 cut(s)	466, 1180, 1455, 2019, 2617, 3057
AfiI	CCNNNNNNNGG	5 cut(s)	721, 1698, 2483, 2861, 2990
AflIII	ACRYGT	1 cut(s)	409
AhdI	GACNNNNNGTC	1 cut(s)	3010
AhlI	ACTAGT	1 cut(s)	1847
AjnI	CCWGG	1 cut(s)	2467
AjuI	GAANNNNNNNTTGG	4 cut(s)	1714, 1746, 1835, 1867
Alw21I	GWGCWC	2 cut(s)	2047, 2351
Alw26I	GTCTC	2 cut(s)	682, 1322
AlwNI	CAGNNNCTG	2 cut(s)	2624, 2954
Ama87I	CYCGRG	2 cut(s)	347, 2872
AoxI	GGCC	4 cut(s)	40, 800, 920, 1158
ApeKI	GCWGC	3 cut(s)	58, 725, 2427
ApoI	RAATTY	6 cut(s)	907, 1146, 2310, 2440, 2880, 2921
ArsI	GACNNNNNNTTYG	1 cut(s)	3097
Asp700I	GAANNNNTTC	2 cut(s)	1431, 2925
Asp718I	GGTACC	1 cut(s)	464
AspLEI	GCGC	2 cut(s)	826, 3082
AspS9I	GGNCC	3 cut(s)	200, 886, 1826
AsuHPI	GGTGA	2 cut(s)	1337, 2513
AsuII	TTCGAA	1 cut(s)	2314
AvaI	CYCGRG	2 cut(s)	347, 2872
AvaII	GGWCC	3 cut(s)	200, 886, 1826
BaeGI	GKGCMC	1 cut(s)	1352
BaeI	ACNNNNGTAYC	4 cut(s)	249, 282, 2001, 2034
BamHI	GGATCC	1 cut(s)	2410
BanI	GGYRCC	1 cut(s)	464
BanII	GRGCYC	1 cut(s)	2351
BarI	GAAGNNNNNNTAC	2 cut(s)	1931, 1963
BbsI	GAAGAC	4 cut(s)	19, 2085, 3009, 3031
Bbv12I	GWGCWC	2 cut(s)	2047, 2351
BbvCI	CCTCAGC	1 cut(s)	1343
BbvI	GCAGC	3 cut(s)	70, 737, 2439
BccI	CCATC	6 cut(s)	281, 455, 1381, 1609, 2295, 2798
BciT130I	CCWGG	1 cut(s)	2469
BciVI	GTATCC	2 cut(s)	364, 1696
BclI	TGATCA	1 cut(s)	1002
BcoDI	GTCTC	2 cut(s)	682, 1322
BcuI	ACTAGT	1 cut(s)	1847
BfaI	CTAG	7 cut(s)	137, 500, 981, 1193, 1593, 1848, 2190
BfmI	CTRYAG	4 cut(s)	1182, 2425, 2652, 2772
BfuI	GTATCC	2 cut(s)	364, 1696
BglII	AGATCT	1 cut(s)	1595
BisI	GCNGC	5 cut(s)	43, 59, 726, 2428, 2898
BlpI	GCTNAGC	2 cut(s)	226, 2889
BlsI	GCNGC	5 cut(s)	44, 60, 727, 2429, 2899
BmcAI	AGTACT	1 cut(s)	1180
Bme1390I	CCNGG	1 cut(s)	2469
Bme18I	GGWCC	3 cut(s)	200, 886, 1826
BmeRI	GACNNNNNGTC	1 cut(s)	3010
BmeT110I	CYCGRG	2 cut(s)	347, 2872
BmgT120I	GGNCC	3 cut(s)	200, 886, 1826
BmiI	GGNNCC	6 cut(s)	466, 887, 1029, 1620, 2412, 2556
BmrFI	CCNGG	1 cut(s)	2469
BmrI	ACTGGG	1 cut(s)	890
BmsI	GCATC	6 cut(s)	251, 745, 1274, 2128, 2678, 2758
BmuI	ACTGGG	1 cut(s)	890
BpiI	GAAGAC	4 cut(s)	19, 2085, 3009, 3031
Bpu10I	CCTNAGC	1 cut(s)	1343
Bpu1102I	GCTNAGC	2 cut(s)	226, 2889
Bpu14I	TTCGAA	1 cut(s)	2314
BpuEI	CTTGAG	3 cut(s)	1457, 1589, 3050
BsaAI	YACGTR	1 cut(s)	2743
BsaBI	GATNNNNATC	1 cut(s)	1391
BsaHI	GRCGYC	1 cut(s)	12
BsaJI	CCNNGG	4 cut(s)	1321, 1403, 1586, 2638
BsaWI	WCCGGW	1 cut(s)	2983
BsaXI	ACNNNNNCTCC	2 cut(s)	1582, 1612
Bsc4I	CCNNNNNNNGG	5 cut(s)	721, 1698, 2483, 2861, 2990
Bse3DI	GCAATG	2 cut(s)	1294, 2540
Bse8I	GATNNNNATC	1 cut(s)	1391
BseBI	CCWGG	1 cut(s)	2469
BseDI	CCNNGG	4 cut(s)	1321, 1403, 1586, 2638
BseGI	GGATG	5 cut(s)	1212, 1886, 2119, 2580, 2809
BseJI	GATNNNNATC	1 cut(s)	1391
BseLI	CCNNNNNNNGG	5 cut(s)	721, 1698, 2483, 2861, 2990
BseMI	GCAATG	2 cut(s)	1294, 2540
BseMII	CTCAG	7 cut(s)	921, 1334, 1389, 1595, 2334, 2903, 2948
BseRI	GAGGAG	2 cut(s)	67, 70
BseSI	GKGCMC	1 cut(s)	1352
BseXI	GCAGC	3 cut(s)	70, 737, 2439
BseYI	CCCAGC	1 cut(s)	2624
BsgI	GTGCAG	1 cut(s)	2456
Bsh1236I	CGCG	2 cut(s)	45, 826
BshFI	GGCC	4 cut(s)	42, 802, 922, 1160
BshNI	GGYRCC	1 cut(s)	464
BsiHKAI	GWGCWC	2 cut(s)	2047, 2351
BsiHKCI	CYCGRG	2 cut(s)	347, 2872
BsiSI	CCGG	2 cut(s)	715, 2984
BslFI	GGGAC	2 cut(s)	899, 2215
BslI	CCNNNNNNNGG	5 cut(s)	721, 1698, 2483, 2861, 2990
BsmAI	GTCTC	2 cut(s)	682, 1322
BsmFI	GGGAC	2 cut(s)	899, 2215
BsnI	GGCC	4 cut(s)	42, 802, 922, 1160
BsoBI	CYCGRG	2 cut(s)	347, 2872
Bsp119I	TTCGAA	1 cut(s)	2314
Bsp1286I	GDGCHC	3 cut(s)	1352, 2047, 2351
Bsp1720I	GCTNAGC	2 cut(s)	226, 2889
Bsp19I	CCATGG	1 cut(s)	1586
BspACI	CCGC	5 cut(s)	43, 826, 1218, 2898, 3034
BspANI	GGCC	4 cut(s)	42, 802, 922, 1160
BspCNI	CTCAG	7 cut(s)	922, 1335, 1390, 1594, 2335, 2902, 2947
BspFNI	CGCG	2 cut(s)	45, 826
BspHI	TCATGA	1 cut(s)	2244
BspLI	GGNNCC	6 cut(s)	466, 887, 1029, 1620, 2412, 2556
BspMAI	CTGCAG	1 cut(s)	2429
BspT104I	TTCGAA	1 cut(s)	2314
BspT107I	GGYRCC	1 cut(s)	464
BsrBI	CCGCTC	1 cut(s)	3034
BsrDI	GCAATG	2 cut(s)	1294, 2540
BssECI	CCNNGG	4 cut(s)	1321, 1403, 1586, 2638
BssNI	GRCGYC	1 cut(s)	12
BssT1I	CCWWGG	4 cut(s)	1321, 1403, 1586, 2638
Bst2UI	CCWGG	1 cut(s)	2469
Bst4CI	ACNGT	8 cut(s)	187, 262, 1183, 1366, 2084, 2254, 2620, 3055
Bst6I	CTCTTC	2 cut(s)	2360, 2860
BstACI	GRCGYC	1 cut(s)	12
BstBAI	YACGTR	1 cut(s)	2743
BstBI	TTCGAA	1 cut(s)	2314
BstC8I	GCNNGC	6 cut(s)	480, 1162, 1770, 1955, 2856, 2895
BstDSI	CCRYGG	1 cut(s)	1586
BstENI	CCTNNNNNAGG	1 cut(s)	1696
BstF5I	GGATG	5 cut(s)	1212, 1886, 2119, 2580, 2809
BstFNI	CGCG	2 cut(s)	45, 826
BstHHI	GCGC	2 cut(s)	826, 3082
BstMAI	GTCTC	2 cut(s)	682, 1322
BstMWI	GCNNNNNNNGC	5 cut(s)	479, 484, 1769, 1778, 2894
BstNI	CCWGG	1 cut(s)	2469
BstNSI	RCATGY	2 cut(s)	482, 3010
BstSCI	CCNGG	1 cut(s)	2467
BstSFI	CTRYAG	4 cut(s)	1182, 2425, 2652, 2772
BstSLI	GKGCMC	1 cut(s)	1352
BstUI	CGCG	2 cut(s)	45, 826
BstV1I	GCAGC	3 cut(s)	70, 737, 2439
BstV2I	GAAGAC	4 cut(s)	19, 2085, 3009, 3031
BstX2I	RGATCY	4 cut(s)	1573, 1595, 1684, 2410
BstYI	RGATCY	4 cut(s)	1573, 1595, 1684, 2410
BsuI	GTATCC	2 cut(s)	364, 1696
BsuRI	GGCC	4 cut(s)	42, 802, 922, 1160
BtgI	CCRYGG	1 cut(s)	1586
BtsCI	GGATG	5 cut(s)	1212, 1886, 2119, 2580, 2809
BtsI	GCAGTG	1 cut(s)	522
BtsIMutI	CAGTG	8 cut(s)	258, 522, 581, 878, 998, 1093, 1395, 2337
Cac8I	GCNNGC	6 cut(s)	480, 1162, 1770, 1955, 2856, 2895
CaiI	CAGNNNCTG	2 cut(s)	2624, 2954
CciI	TCATGA	1 cut(s)	2244
CfoI	GCGC	2 cut(s)	826, 3082
Cfr13I	GGNCC	3 cut(s)	200, 886, 1826
CseI	GACGC	2 cut(s)	839, 3101
Csp6I	GTAC	6 cut(s)	465, 1179, 1454, 2018, 2616, 3056
CspCI	CAANNNNNGTGG	2 cut(s)	1461, 1496
CviQI	GTAC	6 cut(s)	465, 1179, 1454, 2018, 2616, 3056
DraI	TTTAAA	1 cut(s)	2400
DrdI	GACNNNNNNGTC	2 cut(s)	11, 14
DriI	GACNNNNNGTC	1 cut(s)	3010
DseDI	GACNNNNNNGTC	2 cut(s)	11, 14
Eam1104I	CTCTTC	2 cut(s)	2360, 2860
Eam1105I	GACNNNNNGTC	1 cut(s)	3010
EarI	CTCTTC	2 cut(s)	2360, 2860
Ecl136II	GAGCTC	1 cut(s)	2349
Eco130I	CCWWGG	4 cut(s)	1321, 1403, 1586, 2638
Eco147I	AGGCCT	2 cut(s)	802, 1160
Eco24I	GRGCYC	1 cut(s)	2351
Eco32I	GATATC	1 cut(s)	565
Eco47I	GGWCC	3 cut(s)	200, 886, 1826
Eco53kI	GAGCTC	1 cut(s)	2349
Eco57I	CTGAAG	3 cut(s)	1395, 1933, 2097
Eco88I	CYCGRG	2 cut(s)	347, 2872
EcoICRI	GAGCTC	1 cut(s)	2349
EcoNI	CCTNNNNNAGG	1 cut(s)	1696
EcoRI	GAATTC	1 cut(s)	2880
EcoRII	CCWGG	1 cut(s)	2467
EcoRV	GATATC	1 cut(s)	565
EcoT14I	CCWWGG	4 cut(s)	1321, 1403, 1586, 2638
EcoT38I	GRGCYC	1 cut(s)	2351
ErhI	CCWWGG	4 cut(s)	1321, 1403, 1586, 2638
FalI	AAGNNNNNCTT	4 cut(s)	11, 43, 162, 194
FaqI	GGGAC	2 cut(s)	899, 2215
FauI	CCCGC	1 cut(s)	1211
FbaI	TGATCA	1 cut(s)	1002
Fnu4HI	GCNGC	5 cut(s)	43, 59, 726, 2428, 2898
FokI	GGATG	5 cut(s)	1219, 1893, 2106, 2567, 2816
FriOI	GRGCYC	1 cut(s)	2351
Fsp4HI	GCNGC	5 cut(s)	43, 59, 726, 2428, 2898
FspBI	CTAG	7 cut(s)	137, 500, 981, 1193, 1593, 1848, 2190
GlaI	GCGC	2 cut(s)	825, 3081
GluI	GCNGC	5 cut(s)	43, 59, 726, 2428, 2898
GsaI	CCCAGC	1 cut(s)	2628
HaeIII	GGCC	4 cut(s)	42, 802, 922, 1160
HapII	CCGG	2 cut(s)	715, 2984
HgaI	GACGC	2 cut(s)	839, 3101
HhaI	GCGC	2 cut(s)	826, 3082
Hin1I	GRCGYC	1 cut(s)	12
Hin6I	GCGC	2 cut(s)	824, 3080
HinP1I	GCGC	2 cut(s)	824, 3080
HincII	GTYRAC	2 cut(s)	901, 2493
HindII	GTYRAC	2 cut(s)	901, 2493
HindIII	AAGCTT	3 cut(s)	223, 485, 2462
HinfI	GANTC	9 cut(s)	24, 379, 572, 1210, 1432, 1730, 2726, 2780, 2957
HpaI	GTTAAC	1 cut(s)	2493
HpaII	CCGG	2 cut(s)	715, 2984
HphI	GGTGA	2 cut(s)	1337, 2513
Hpy166II	GTNNAC	4 cut(s)	581, 901, 1454, 2493
Hpy8I	GTNNAC	4 cut(s)	581, 901, 1454, 2493
Hpy99I	CGWCG	4 cut(s)	11, 14, 17, 2965
HpyCH4III	ACNGT	8 cut(s)	187, 262, 1183, 1366, 2084, 2254, 2620, 3055
HpyCH4IV	ACGT	3 cut(s)	12, 409, 2742
HpyF10VI	GCNNNNNNNGC	5 cut(s)	479, 484, 1769, 1778, 2894
HpySE526I	ACGT	3 cut(s)	12, 409, 2742
Hsp92I	GRCGYC	1 cut(s)	12
HspAI	GCGC	2 cut(s)	824, 3080
KpnI	GGTACC	1 cut(s)	468
Ksp22I	TGATCA	1 cut(s)	1002
KspAI	GTTAAC	1 cut(s)	2493
LmnI	GCTCC	4 cut(s)	1169, 1618, 1723, 2554
Lsp1109I	GCAGC	3 cut(s)	70, 737, 2439
LweI	GCATC	6 cut(s)	251, 745, 1274, 2128, 2678, 2758
MaeI	CTAG	7 cut(s)	137, 500, 981, 1193, 1593, 1848, 2190
MaeII	ACGT	3 cut(s)	12, 409, 2742
MaeIII	GTNAC	9 cut(s)	181, 876, 1088, 1221, 1253, 1299, 1558, 2369, 2501
MbiI	CCGCTC	1 cut(s)	3034
MboII	GAAGA	9 cut(s)	19, 830, 1396, 2090, 2377, 2822, 2877, 3014, 3036
MflI	RGATCY	4 cut(s)	1573, 1595, 1684, 2410
MhlI	GDGCHC	3 cut(s)	1352, 2047, 2351
MlyI	GAGTC	2 cut(s)	33, 1204
MmeI	TCCRAC	7 cut(s)	1180, 1348, 1625, 1767, 1795, 2143, 2608
MroXI	GAANNNNTTC	2 cut(s)	1431, 2925
MseI	TTAA	9 cut(s)	663, 1464, 1926, 2058, 2399, 2492, 2634, 2796, 3071
MspI	CCGG	2 cut(s)	715, 2984
MspR9I	CCNGG	1 cut(s)	2469
MvaI	CCWGG	1 cut(s)	2469
MvnI	CGCG	2 cut(s)	45, 826
MwoI	GCNNNNNNNGC	5 cut(s)	479, 484, 1769, 1778, 2894
NcoI	CCATGG	1 cut(s)	1586
NlaIV	GGNNCC	6 cut(s)	466, 887, 1029, 1620, 2412, 2556
NmeAIII	GCCGAG	1 cut(s)	1543
NmuCI	GTSAC	4 cut(s)	876, 1088, 1299, 2501
NspI	RCATGY	2 cut(s)	482, 3010
NspV	TTCGAA	1 cut(s)	2314
PaeI	GCATGC	1 cut(s)	482
PaeR7I	CTCGAG	1 cut(s)	347
PagI	TCATGA	1 cut(s)	2244
PceI	AGGCCT	2 cut(s)	802, 1160
PcsI	WCGNNNNNNNCGW	1 cut(s)	12
PdmI	GAANNNNTTC	2 cut(s)	1431, 2925
PfeI	GAWTC	7 cut(s)	379, 572, 1432, 1730, 2726, 2780, 2957
PflFI	GACNNNGTC	2 cut(s)	11, 14
PfoI	TCCNGGA	1 cut(s)	2467
PkrI	GCNGC	5 cut(s)	44, 60, 727, 2429, 2899
PleI	GAGTC	2 cut(s)	32, 1204
PpsI	GAGTC	2 cut(s)	32, 1204
Ppu21I	YACGTR	1 cut(s)	2743
PsiI	TTATAA	1 cut(s)	1911
Psp124BI	GAGCTC	1 cut(s)	2351
Psp6I	CCWGG	1 cut(s)	2467
PspFI	CCCAGC	1 cut(s)	2624
PspGI	CCWGG	1 cut(s)	2467
PspN4I	GGNNCC	6 cut(s)	466, 887, 1029, 1620, 2412, 2556
PspPI	GGNCC	3 cut(s)	200, 886, 1826
PstI	CTGCAG	1 cut(s)	2429
PstNI	CAGNNNCTG	2 cut(s)	2624, 2954
PsuI	RGATCY	4 cut(s)	1573, 1595, 1684, 2410
PsyI	GACNNNGTC	2 cut(s)	11, 14
RsaI	GTAC	6 cut(s)	466, 1180, 1455, 2019, 2617, 3057
RsaNI	GTAC	6 cut(s)	465, 1179, 1454, 2018, 2616, 3056
SacI	GAGCTC	1 cut(s)	2351
SaqAI	TTAA	9 cut(s)	663, 1464, 1926, 2058, 2399, 2492, 2634, 2796, 3071
SatI	GCNGC	5 cut(s)	43, 59, 726, 2428, 2898
Sau96I	GGNCC	3 cut(s)	200, 886, 1826
ScaI	AGTACT	1 cut(s)	1180
SchI	GAGTC	2 cut(s)	33, 1204
ScrFI	CCNGG	1 cut(s)	2469
SduI	GDGCHC	3 cut(s)	1352, 2047, 2351
SfaNI	GCATC	6 cut(s)	251, 745, 1274, 2128, 2678, 2758
SfcI	CTRYAG	4 cut(s)	1182, 2425, 2652, 2772
Sfr274I	CTCGAG	1 cut(s)	347
SfuI	TTCGAA	1 cut(s)	2314
SinI	GGWCC	3 cut(s)	200, 886, 1826
SlaI	CTCGAG	1 cut(s)	347
SmlI	CTYRAG	4 cut(s)	347, 1436, 1568, 3029
SmoI	CTYRAG	4 cut(s)	347, 1436, 1568, 3029
SpeI	ACTAGT	1 cut(s)	1847
SphI	GCATGC	1 cut(s)	482
SseBI	AGGCCT	2 cut(s)	802, 1160
SsiI	CCGC	5 cut(s)	43, 826, 1218, 2898, 3034
SspMI	CTAG	7 cut(s)	137, 500, 981, 1193, 1593, 1848, 2190
SstI	GAGCTC	1 cut(s)	2351
StuI	AGGCCT	2 cut(s)	802, 1160
StyD4I	CCNGG	1 cut(s)	2467
StyI	CCWWGG	4 cut(s)	1321, 1403, 1586, 2638
TaaI	ACNGT	8 cut(s)	187, 262, 1183, 1366, 2084, 2254, 2620, 3055
TaiI	ACGT	3 cut(s)	15, 412, 2745
TatI	WGTACW	2 cut(s)	1178, 2615
TauI	GCSGC	2 cut(s)	45, 2900
TfiI	GAWTC	7 cut(s)	379, 572, 1432, 1730, 2726, 2780, 2957
Tru1I	TTAA	9 cut(s)	663, 1464, 1926, 2058, 2399, 2492, 2634, 2796, 3071
Tru9I	TTAA	9 cut(s)	663, 1464, 1926, 2058, 2399, 2492, 2634, 2796, 3071
TscAI	CASTG	8 cut(s)	265, 522, 588, 885, 1005, 1093, 1402, 2337
TseFI	GTSAC	4 cut(s)	876, 1088, 1299, 2501
TseI	GCWGC	3 cut(s)	58, 725, 2427
Tsp45I	GTSAC	4 cut(s)	876, 1088, 1299, 2501
TspGWI	ACGGA	1 cut(s)	138
TspRI	CASTG	8 cut(s)	265, 522, 588, 885, 1005, 1093, 1402, 2337
Tth111I	GACNNNGTC	2 cut(s)	11, 14
VpaK11BI	GGWCC	3 cut(s)	200, 886, 1826
XagI	CCTNNNNNAGG	1 cut(s)	1696
XapI	RAATTY	6 cut(s)	907, 1146, 2310, 2440, 2880, 2921
XbaI	TCTAGA	2 cut(s)	1192, 2189
XceI	RCATGY	2 cut(s)	482, 3010
XhoI	CTCGAG	1 cut(s)	347
XmnI	GAANNNNTTC	2 cut(s)	1431, 2925
XspI	CTAG	7 cut(s)	137, 500, 981, 1193, 1593, 1848, 2190
ZraI	GACGTC	1 cut(s)	13
ZrmI	AGTACT	1 cut(s)	1180

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.