MD13G1027000.v1.1

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0016044
Physical Location & Seq
Reverse (-)
1958054 .. 1959625
1572 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1027000.v1.1.491

Sequence Viewer

Length: 1572 bp
ATGGGTCTCTCTCGAATTTTCCCTCTTGTGTCGAAACTATCCAAAAACTTCATTCGCAATCGAACACTCCCTACCGAAAATCCATCCGGGTTCCTCTGCAACCGCACTGCTAGTTTTCACAGAGCAATCCACGACGGTCTGGAAAAACCCCAAGTGCTCCATGACGCCGGTGACAAGACCCCACTTCTCAAAAACCCCAAAGTCTCAGAAGATGCTGAGAAAATCTGTAAAATTCTATCCACCAGCAGCTCTAATTCCCCAATCGAGAGCTTCCTCGACGGCGCTTCGGTCGAGGCGTCGCCGACGCTTGTGGTGGAGGTCTTGAAGAAGCTCAGCAACGCCGGCGTCCTCGCCCTCTCGTTTTTCCGGTGGGCGGAGAAGCAGAAAGGGTTTAAGCACACCACGGAGAGCTACAATGCGTTGATCGAAGCTCTGGGTAAGATCAAGCAGTTCAAGATGATGTGGGAATTGGTGAATGAAATGAAGATCAAAGGGATGCTGAGTAAAGAGACTTTCGCGCTGATTTCACGGCGATATGCGAGGGTGAAGAAGGTTAAGGAAGCCATTGATGCGTTCGAGAAGATGGCGAAGTTTGGAATGAAGGTGGAAGGGTCAGATTTCAATAGATTGATTGATACTCTGAGCAAGTCGAGGCAGGTTGAGAGAGCACAGGAGGTGTTTGATAAAATGAAGAAGAGAAGGTTTGAACCCGATATCAAGTCGTACACGATTTTATTGGAAGGATGGGGCCAAGAACAGAACTTTTTGAGGTTGAATGAGGTTTATCGAGAGATGAAGGACGAGGGTTTTGATGCGGATGTTGTGACATATGCTATTCTGATCAATGCACATTGTAAAGCGAAGAAGTACGATGAAGCGATTGACTTGTTTCGCGAGATGGAGGCAAAGAATATCAAGGCTACTCCTCATATATTCTGCATTTTGATTAATGGGTTGGGTTCTGAAAAGAGGCTGAGTGAAGCCCTTGAGTTTTTTGAACTGAACAAAGCCAGTGGTTTCAAACCGGAGGCACCCACGTACAATGCTCTTGTGGGGTCTTACTGCTGGTCGATGCGGATGCAGGATGCGTTTAGGGTGGTGGATGAGATGAGGAAATGTGGAATCGGTCCCAATGCCCGAACTTACGACATAATCTTGCATCACTTGGTAAAGGCTCGAAGAACCGAACAAGCTTACTCCATTTTCCAGCAAATGAGTAGGGATCCTGGCTGTGAGCCAACAGTAAGTACGTATGAAATCATGGTGAGGATGTTTTGTAACGAGGAGCGCATGGATATGGCATTGCAGGTTTGGGATCAGATGAAGACCAGAGGAGTCCTGCCGGGAATGCACATGTTCGCCACATTGATTAACAGCTTGTGTCATGGGAATAAGTTAGATGACGCTTGCAAGTACTTTCAAGAGATGCTGGATGCCGGTATTCGACCTCCAGCGCAGTTGTTTAGTAATTTGAAACAATCGCTTCTCGACGGGGGAAGAAAGGATGTTGTGATAAGTTTGGGTCTGAAGATTGACAGACTGAGGAAAACTCCGTTAGTTGGGTTGCGCTAA

Protein Analysis

524

Amino Acids

59.68

Weight (kDa)

9.18

Isoelectric Point (pI)

39.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TPR_24 PF23276 106 - 229 4.2e-06 Fungal tetratrico peptide repeats
PPR_long PF17177 180 - 308 8.4e-09 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 181 - 300 2.8e-09 Fungal tetratrico peptide repeats
PPR_3 PF13812 192 - 250 1.5e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 207 - 248 6.1e-11 PPR repeat family
PPR_2 PF13041 259 - 286 2.3e-06 PPR repeat family
PPR_3 PF13812 261 - 320 2.7e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 269 - 301 3.8e-11 PPR repeat
PPR_2 PF13041 273 - 319 8.5e-13 PPR repeat family
PPR PF01535 275 - 305 4.2e-08 PPR repeat
PPR_long PF17177 292 - 406 1.6e-10 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 331 - 407 3.5e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 336 - 389 6e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 339 - 371 8e-07 PPR repeat
PPR_2 PF13041 345 - 391 6.7e-12 PPR repeat family
PPR PF01535 346 - 375 8.3e-06 PPR repeat
PPR_3 PF13812 365 - 427 7.2e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 381 - 427 5.4e-10 PPR repeat family
PPR_long PF17177 397 - 499 4.7e-06 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 410 - 442 7.1e-08 PPR repeat
PPR_2 PF13041 420 - 462 1.6e-07 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016044)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71060
fragaria_vesca FvH4_4g32230
malus_domestica MD13G1027000.v1.1 MD16G1030100.v1.1
prunus_persica Prupe.1G322900_v2.0.a1
pyrus_communis pycom13g02570
rosa_chinensis RchiOBHm_Chr4g0440971
rosa_laevigata RLG00000006135
rosa_multiflora Rmu_sc0000424.1_g000028
rosa_roxburghii Rroxscaffold_5G00381830
rosa_rugosa Rorug04G0329800
rosa_samantha Rh4BG394900 Rh4CG409800 Rh4DG388100
rosa_wichuraiana Rw4G032870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 646, 1297
AccB1I GGYRCC 1 cut(s) 1030
AccII CGCG 2 cut(s) 518, 894
AciI CCGC 4 cut(s) 103, 374, 815, 1075
AclWI GGATC 3 cut(s) 1217, 1230, 1323
AcsI RAATTY 2 cut(s) 15, 231
AcuI CTGAAG 1 cut(s) 1547
AcyI GRCGYC 3 cut(s) 165, 296, 345
AfaI GTAC 5 cut(s) 725, 869, 1040, 1249, 1415
AfiI CCNNNNNNNGG 2 cut(s) 373, 1559
AflIII ACRYGT 1 cut(s) 1353
AgsI TTSAA 9 cut(s) 325, 454, 622, 707, 775, 998, 1021, 1421, 1474
AjnI CCWGG 1 cut(s) 1225
AluBI AGCT 7 cut(s) 249, 270, 331, 411, 431, 1193, 1377
AluI AGCT 7 cut(s) 249, 270, 331, 411, 431, 1193, 1377
Alw21I GWGCWC 2 cut(s) 159, 670
Alw26I GTCTC 3 cut(s) 11, 208, 503
AlwI GGATC 3 cut(s) 1217, 1230, 1323
AoxI GGCC 1 cut(s) 748
ApeKI GCWGC 1 cut(s) 246
ApoI RAATTY 2 cut(s) 15, 231
ArsI GACNNNNNNTTYG 2 cut(s) 791, 823
AseI ATTAAT 1 cut(s) 948
AspLEI GCGC 5 cut(s) 284, 520, 1290, 1456, 1569
AspS9I GGNCC 2 cut(s) 748, 1127
AsuC2I CCSGG 2 cut(s) 88, 1344
AsuHPI GGTGA 4 cut(s) 182, 484, 556, 1276
AvaII GGWCC 1 cut(s) 1127
BamHI GGATCC 1 cut(s) 1222
BanI GGYRCC 1 cut(s) 1030
BbsI GAAGAC 1 cut(s) 1331
Bbv12I GWGCWC 2 cut(s) 159, 670
BbvI GCAGC 1 cut(s) 258
BccI CCATC 4 cut(s) 91, 577, 738, 892
BceAI ACGGC 2 cut(s) 295, 545
BcgI CGANNNNNNTGC 2 cut(s) 1060, 1094
BciT130I CCWGG 1 cut(s) 1227
BclI TGATCA 1 cut(s) 840
BcnI CCSGG 2 cut(s) 88, 1344
BcoDI GTCTC 3 cut(s) 11, 208, 503
BfaI CTAG 1 cut(s) 111
BfoI RGCGCY 1 cut(s) 285
BfuAI ACCTGC 2 cut(s) 646, 1297
BisI GCNGC 1 cut(s) 247
BlpI GCTNAGC 1 cut(s) 332
BlsI GCNGC 1 cut(s) 248
BmcAI AGTACT 1 cut(s) 1415
Bme1390I CCNGG 3 cut(s) 88, 1227, 1344
Bme18I GGWCC 1 cut(s) 1127
BmgT120I GGNCC 2 cut(s) 748, 1127
BmiI GGNNCC 5 cut(s) 92, 749, 1032, 1129, 1224
BmrFI CCNGG 3 cut(s) 88, 1227, 1344
BpiI GAAGAC 1 cut(s) 1331
BplI GAGNNNNNCTC 2 cut(s) 1534, 1566
BpmI CTGGAG 1 cut(s) 1434
Bpu1102I GCTNAGC 1 cut(s) 332
BpuEI CTTGAG 1 cut(s) 1007
BpuMI CCSGG 2 cut(s) 88, 1344
BsaAI YACGTR 2 cut(s) 1038, 1251
BsaHI GRCGYC 3 cut(s) 165, 296, 345
BsaI GGTCTC 1 cut(s) 11
BsaJI CCNNGG 1 cut(s) 402
BsaWI WCCGGW 2 cut(s) 366, 1024
Bsc4I CCNNNNNNNGG 2 cut(s) 373, 1559
Bse118I RCCGGY 3 cut(s) 167, 341, 1436
Bse1I ACTGG 1 cut(s) 1011
Bse3DI GCAATG 1 cut(s) 1301
BseBI CCWGG 1 cut(s) 1227
BseDI CCNNGG 1 cut(s) 402
BseLI CCNNNNNNNGG 2 cut(s) 373, 1559
BseMI GCAATG 1 cut(s) 1301
BseMII CTCAG 7 cut(s) 207, 219, 346, 491, 632, 965, 1532
BseNI ACTGG 1 cut(s) 1011
BseRI GAGGAG 3 cut(s) 915, 1298, 1347
BseXI GCAGC 1 cut(s) 258
Bsh1236I CGCG 2 cut(s) 518, 894
Bsh1285I CGRYCG 1 cut(s) 291
BshFI GGCC 1 cut(s) 750
BshNI GGYRCC 1 cut(s) 1030
BsiEI CGRYCG 1 cut(s) 291
BsiHKAI GWGCWC 2 cut(s) 159, 670
BsiSI CCGG 7 cut(s) 87, 168, 342, 367, 1025, 1343, 1437
BslFI GGGAC 1 cut(s) 1113
BslI CCNNNNNNNGG 2 cut(s) 373, 1559
BsmAI GTCTC 3 cut(s) 11, 208, 503
BsmFI GGGAC 1 cut(s) 1113
BsmI GAATGC 1 cut(s) 1353
BsnI GGCC 1 cut(s) 750
Bso31I GGTCTC 1 cut(s) 11
Bsp1286I GDGCHC 2 cut(s) 159, 670
Bsp143I GATC 6 cut(s) 423, 441, 486, 840, 1222, 1315
Bsp1720I GCTNAGC 1 cut(s) 332
Bsp68I TCGCGA 1 cut(s) 894
BspACI CCGC 4 cut(s) 103, 374, 815, 1075
BspANI GGCC 1 cut(s) 750
BspCNI CTCAG 7 cut(s) 208, 218, 345, 492, 633, 966, 1533
BspFNI CGCG 2 cut(s) 518, 894
BspLI GGNNCC 5 cut(s) 92, 749, 1032, 1129, 1224
BspMI ACCTGC 2 cut(s) 646, 1297
BspPI GGATC 3 cut(s) 1217, 1230, 1323
BspT107I GGYRCC 1 cut(s) 1030
BspTNI GGTCTC 1 cut(s) 11
BsrDI GCAATG 1 cut(s) 1301
BsrFI RCCGGY 3 cut(s) 167, 341, 1436
BsrI ACTGG 1 cut(s) 1011
BssAI RCCGGY 3 cut(s) 167, 341, 1436
BssECI CCNNGG 1 cut(s) 402
BssMI GATC 6 cut(s) 423, 441, 486, 840, 1222, 1315
BssNI GRCGYC 3 cut(s) 165, 296, 345
Bst2UI CCWGG 1 cut(s) 1227
Bst4CI ACNGT 2 cut(s) 137, 1243
Bst6I CTCTTC 1 cut(s) 689
BstACI GRCGYC 3 cut(s) 165, 296, 345
BstBAI YACGTR 2 cut(s) 1038, 1251
BstC8I GCNNGC 2 cut(s) 343, 1408
BstDEI CTNAG 7 cut(s) 205, 216, 332, 500, 641, 974, 1541
BstDSI CCRYGG 1 cut(s) 402
BstFNI CGCG 2 cut(s) 518, 894
BstH2I RGCGCY 1 cut(s) 285
BstHHI GCGC 5 cut(s) 284, 520, 1290, 1456, 1569
BstKTI GATC 6 cut(s) 426, 444, 489, 843, 1225, 1318
BstMAI GTCTC 3 cut(s) 11, 208, 503
BstMBI GATC 6 cut(s) 423, 441, 486, 840, 1222, 1315
BstMCI CGRYCG 1 cut(s) 291
BstMWI GCNNNNNNNGC 3 cut(s) 342, 569, 1348
BstNI CCWGG 1 cut(s) 1227
BstNSI RCATGY 1 cut(s) 1357
BstSCI CCNGG 3 cut(s) 86, 1225, 1342
BstSNI TACGTA 1 cut(s) 1251
BstUI CGCG 2 cut(s) 518, 894
BstV1I GCAGC 1 cut(s) 258
BstV2I GAAGAC 1 cut(s) 1331
BstX2I RGATCY 1 cut(s) 1222
BstYI RGATCY 1 cut(s) 1222
BsuRI GGCC 1 cut(s) 750
BtgI CCRYGG 1 cut(s) 402
BtsI GCAGTG 1 cut(s) 105
BtsIMutI CAGTG 2 cut(s) 105, 1018
BtuMI TCGCGA 1 cut(s) 894
BveI ACCTGC 2 cut(s) 646, 1297
Cac8I GCNNGC 2 cut(s) 343, 1408
CfoI GCGC 5 cut(s) 284, 520, 1290, 1456, 1569
Cfr10I RCCGGY 3 cut(s) 167, 341, 1436
Cfr13I GGNCC 2 cut(s) 748, 1127
CseI GACGC 5 cut(s) 173, 285, 313, 334, 1412
Csp6I GTAC 5 cut(s) 724, 868, 1039, 1248, 1414
CspCI CAANNNNNGTGG 2 cut(s) 994, 1029
CviAII CATG 5 cut(s) 161, 1261, 1291, 1354, 1385
CviQI GTAC 5 cut(s) 724, 868, 1039, 1248, 1414
DdeI CTNAG 7 cut(s) 205, 216, 332, 500, 641, 974, 1541
DpnI GATC 6 cut(s) 425, 443, 488, 842, 1224, 1317
DpnII GATC 6 cut(s) 423, 441, 486, 840, 1222, 1315
Eam1104I CTCTTC 1 cut(s) 689
EarI CTCTTC 1 cut(s) 689
EciI GGCGGA 1 cut(s) 389
Eco105I TACGTA 1 cut(s) 1251
Eco31I GGTCTC 1 cut(s) 11
Eco32I GATATC 1 cut(s) 715
Eco47I GGWCC 1 cut(s) 1127
Eco57I CTGAAG 1 cut(s) 1547
EcoRII CCWGG 1 cut(s) 1225
EcoRV GATATC 1 cut(s) 715
FaeI CATG 5 cut(s) 164, 1264, 1294, 1357, 1388
FaqI GGGAC 1 cut(s) 1113
FatI CATG 5 cut(s) 160, 1260, 1290, 1353, 1384
FauNDI CATATG 1 cut(s) 829
FbaI TGATCA 1 cut(s) 840
Fnu4HI GCNGC 1 cut(s) 247
Fsp4HI GCNGC 1 cut(s) 247
FspBI CTAG 1 cut(s) 111
GlaI GCGC 5 cut(s) 283, 519, 1289, 1455, 1568
GluI GCNGC 1 cut(s) 247
GsuI CTGGAG 1 cut(s) 1434
HaeII RGCGCY 1 cut(s) 285
HaeIII GGCC 1 cut(s) 750
HapII CCGG 7 cut(s) 87, 168, 342, 367, 1025, 1343, 1437
HgaI GACGC 5 cut(s) 173, 285, 313, 334, 1412
HhaI GCGC 5 cut(s) 284, 520, 1290, 1456, 1569
Hin1I GRCGYC 3 cut(s) 165, 296, 345
Hin1II CATG 5 cut(s) 164, 1264, 1294, 1357, 1388
Hin6I GCGC 5 cut(s) 282, 518, 1288, 1454, 1567
HinP1I GCGC 5 cut(s) 282, 518, 1288, 1454, 1567
HindIII AAGCTT 1 cut(s) 1191
HinfI GANTC 2 cut(s) 1122, 1335
HpaII CCGG 7 cut(s) 87, 168, 342, 367, 1025, 1343, 1437
HphI GGTGA 4 cut(s) 182, 484, 556, 1276
Hpy166II GTNNAC 1 cut(s) 726
Hpy188I TCNGA 7 cut(s) 208, 616, 642, 840, 964, 1320, 1527
Hpy8I GTNNAC 1 cut(s) 726
Hpy99I CGWCG 5 cut(s) 137, 281, 301, 307, 1493
HpyAV CCTTC 6 cut(s) 544, 595, 602, 693, 734, 790
HpyCH4III ACNGT 2 cut(s) 137, 1243
HpyCH4IV ACGT 2 cut(s) 1037, 1250
HpyCH4V TGCA 8 cut(s) 99, 848, 939, 1081, 1159, 1306, 1351, 1410
HpyF10VI GCNNNNNNNGC 3 cut(s) 342, 569, 1348
HpyF3I CTNAG 7 cut(s) 205, 216, 332, 500, 641, 974, 1541
HpySE526I ACGT 2 cut(s) 1037, 1250
Hsp92I GRCGYC 3 cut(s) 165, 296, 345
Hsp92II CATG 5 cut(s) 164, 1264, 1294, 1357, 1388
HspAI GCGC 5 cut(s) 282, 518, 1288, 1454, 1567
KroI GCCGGC 1 cut(s) 341
KroNI GCCGGC 1 cut(s) 343
Ksp22I TGATCA 1 cut(s) 840
Kzo9I GATC 6 cut(s) 423, 441, 486, 840, 1222, 1315
LmnI GCTCC 2 cut(s) 162, 1285
Lsp1109I GCAGC 1 cut(s) 258
MaeI CTAG 1 cut(s) 111
MaeII ACGT 2 cut(s) 1037, 1250
MaeIII GTNAC 3 cut(s) 170, 823, 1277
MalI GATC 6 cut(s) 425, 443, 488, 842, 1224, 1317
MboI GATC 6 cut(s) 423, 441, 486, 840, 1222, 1315
MflI RGATCY 1 cut(s) 1222
MhlI GDGCHC 2 cut(s) 159, 670
MluCI AATT 5 cut(s) 15, 231, 253, 467, 1468
MlyI GAGTC 1 cut(s) 1344
MreI CGCCGGCG 1 cut(s) 341
MroNI GCCGGC 1 cut(s) 341
MseI TTAA 4 cut(s) 393, 555, 948, 1371
MslI CAYNNNNRTG 1 cut(s) 1295
MspI CCGG 7 cut(s) 87, 168, 342, 367, 1025, 1343, 1437
MspR9I CCNGG 3 cut(s) 88, 1227, 1344
Mva1269I GAATGC 1 cut(s) 1353
MvaI CCWGG 1 cut(s) 1227
MvnI CGCG 2 cut(s) 518, 894
MwoI GCNNNNNNNGC 3 cut(s) 342, 569, 1348
NaeI GCCGGC 1 cut(s) 343
NciI CCSGG 2 cut(s) 88, 1344
NdeI CATATG 1 cut(s) 829
NdeII GATC 6 cut(s) 423, 441, 486, 840, 1222, 1315
NgoMIV GCCGGC 1 cut(s) 341
NlaIII CATG 5 cut(s) 164, 1264, 1294, 1357, 1388
NlaIV GGNNCC 5 cut(s) 92, 749, 1032, 1129, 1224
NmuCI GTSAC 2 cut(s) 170, 823
NruI TCGCGA 1 cut(s) 894
NspI RCATGY 1 cut(s) 1357
PciI ACATGT 1 cut(s) 1353
PcsI WCGNNNNNNNCGW 1 cut(s) 293
PctI GAATGC 1 cut(s) 1353
PdiI GCCGGC 1 cut(s) 343
PfeI GAWTC 1 cut(s) 1122
PkrI GCNGC 1 cut(s) 248
PleI GAGTC 1 cut(s) 1343
PpsI GAGTC 1 cut(s) 1343
Ppu21I YACGTR 2 cut(s) 1038, 1251
PscI ACATGT 1 cut(s) 1353
PshBI ATTAAT 1 cut(s) 948
Psp6I CCWGG 1 cut(s) 1225
PspGI CCWGG 1 cut(s) 1225
PspN4I GGNNCC 5 cut(s) 92, 749, 1032, 1129, 1224
PspPI GGNCC 2 cut(s) 748, 1127
PsrI GAACNNNNNNTAC 2 cut(s) 55, 87
PsuI RGATCY 1 cut(s) 1222
RruI TCGCGA 1 cut(s) 894
RsaI GTAC 5 cut(s) 725, 869, 1040, 1249, 1415
RsaNI GTAC 5 cut(s) 724, 868, 1039, 1248, 1414
RseI CAYNNNNRTG 1 cut(s) 1295
SaqAI TTAA 4 cut(s) 393, 555, 948, 1371
SatI GCNGC 1 cut(s) 247
Sau3AI GATC 6 cut(s) 423, 441, 486, 840, 1222, 1315
Sau96I GGNCC 2 cut(s) 748, 1127
ScaI AGTACT 1 cut(s) 1415
SchI GAGTC 1 cut(s) 1344
ScrFI CCNGG 3 cut(s) 88, 1227, 1344
SduI GDGCHC 2 cut(s) 159, 670
SgrAI CRCCGGYG 2 cut(s) 167, 341
SinI GGWCC 1 cut(s) 1127
SmiMI CAYNNNNRTG 1 cut(s) 1295
SmlI CTYRAG 1 cut(s) 986
SmoI CTYRAG 1 cut(s) 986
SnaBI TACGTA 1 cut(s) 1251
Sse9I AATT 5 cut(s) 15, 231, 253, 467, 1468
SsiI CCGC 4 cut(s) 103, 374, 815, 1075
SspMI CTAG 1 cut(s) 111
StyD4I CCNGG 3 cut(s) 86, 1225, 1342
TaaI ACNGT 2 cut(s) 137, 1243
TaiI ACGT 2 cut(s) 1040, 1253
TaqII GACCGA 2 cut(s) 277, 1115
TasI AATT 5 cut(s) 15, 231, 253, 467, 1468
TatI WGTACW 1 cut(s) 1413
TfiI GAWTC 1 cut(s) 1122
Tru1I TTAA 4 cut(s) 393, 555, 948, 1371
Tru9I TTAA 4 cut(s) 393, 555, 948, 1371
TscAI CASTG 2 cut(s) 112, 1018
TseFI GTSAC 2 cut(s) 170, 823
TseI GCWGC 1 cut(s) 246
Tsp45I GTSAC 2 cut(s) 170, 823
TspDTI ATGAA 9 cut(s) 40, 492, 497, 614, 704, 809, 888, 1269, 1337
TspGWI ACGGA 2 cut(s) 419, 1542
TspRI CASTG 2 cut(s) 112, 1018
VpaK11BI GGWCC 1 cut(s) 1127
VspI ATTAAT 1 cut(s) 948
XapI RAATTY 2 cut(s) 15, 231
XceI RCATGY 1 cut(s) 1357
XspI CTAG 1 cut(s) 111
ZrmI AGTACT 1 cut(s) 1415
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.