MD16G1030100.v1.1

endonuclease activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0016044
Physical Location & Seq
Reverse (-)
2158809 .. 2159408
600 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1030100.v1.1.491

Sequence Viewer

Length: 600 bp
ATGGATCTCTCTCGAATTTTCCCTCGTGTATCAAAACTATCCATAAACTTCACTCGCAATCCAACACTCCCTACTCAAAATCCATCTGGGTTCCTATGTGTTCGCGCTGGTAGTTTTCACAGAGCAATCCACGACGGTCTGGAAAAAACCCGAGTGCTGGATGACGCCGGTGACAAAACCCCACTTCTCAAAAACCCCAAAGTCTCAGAAGGCGCTGATAAAATCTGCAAATTTCTATCCACCAGCAGCTCTAGTTCTCCAATCGAGAGCTTACTCAACGGTGCTTCAGTGGACGTCTCGCCGACGCTTGTGGTGGCGGTCTTGAAGAAGTTCAGCAACGCCGGCGTCCTCGCGCTCTCGTTCTTCCGGTGGGTGGAGAAGCGGAAAGGGTTTAAGCACAACACGGAGAGCTACAATGCGTTGATTGAAGCTCTGGGTAAGATCAAACAGTTCAAGATGGTGTGGGAATTGGTGAACGACATGAAGATCAAAGGGCTGCTGAGTAAAGAGACTTTTGCGCTGATTCGCGGCGATATGCGAGGGCGAAGAAGGTTAAGGAAGCCATTGATGCGTTCGAGAAGATGGAGAAGTTTGGAATGA

Protein Analysis

200

Amino Acids

22.46

Weight (kDa)

10.53

Isoelectric Point (pI)

42.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 134 - 166 3.5e-06 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016044)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71060
fragaria_vesca FvH4_4g32230
malus_domestica MD13G1027000.v1.1 MD16G1030100.v1.1
prunus_persica Prupe.1G322900_v2.0.a1
pyrus_communis pycom13g02570
rosa_chinensis RchiOBHm_Chr4g0440971
rosa_laevigata RLG00000006135
rosa_multiflora Rmu_sc0000424.1_g000028
rosa_roxburghii Rroxscaffold_5G00381830
rosa_rugosa Rorug04G0329800
rosa_samantha Rh4BG394900 Rh4CG409800 Rh4DG388100
rosa_wichuraiana Rw4G032870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 297
AccII CGCG 3 cut(s) 105, 353, 528
AciI CCGC 3 cut(s) 317, 382, 528
AclWI GGATC 1 cut(s) 12
AcsI RAATTY 2 cut(s) 15, 230
AcuI CTGAAG 1 cut(s) 270
AcyI GRCGYC 3 cut(s) 165, 294, 345
AfiI CCNNNNNNNGG 2 cut(s) 157, 373
AgsI TTSAA 3 cut(s) 325, 428, 454
AluBI AGCT 4 cut(s) 249, 270, 411, 431
AluI AGCT 4 cut(s) 249, 270, 411, 431
Alw26I GTCTC 3 cut(s) 208, 301, 503
AlwI GGATC 1 cut(s) 12
Ama87I CYCGRG 1 cut(s) 150
ApeKI GCWGC 2 cut(s) 246, 496
ApoI RAATTY 2 cut(s) 15, 230
Asp700I GAANNNNTTC 1 cut(s) 329
AspLEI GCGC 4 cut(s) 107, 215, 355, 520
AsuHPI GGTGA 2 cut(s) 182, 484
AvaI CYCGRG 1 cut(s) 150
BauI CACGAG 1 cut(s) 24
BbvI GCAGC 2 cut(s) 258, 483
BccI CCATC 3 cut(s) 91, 451, 576
BcoDI GTCTC 3 cut(s) 208, 301, 503
BfaI CTAG 1 cut(s) 252
BfoI RGCGCY 1 cut(s) 216
BisI GCNGC 3 cut(s) 247, 497, 529
BlsI GCNGC 3 cut(s) 248, 498, 530
BmeT110I CYCGRG 1 cut(s) 150
BmiI GGNNCC 1 cut(s) 92
BmsI GCATC 1 cut(s) 558
BsaHI GRCGYC 3 cut(s) 165, 294, 345
BsaWI WCCGGW 1 cut(s) 366
Bsc4I CCNNNNNNNGG 2 cut(s) 157, 373
Bse118I RCCGGY 2 cut(s) 167, 341
BseGI GGATG 1 cut(s) 166
BseLI CCNNNNNNNGG 2 cut(s) 157, 373
BseMII CTCAG 2 cut(s) 219, 491
BseXI GCAGC 2 cut(s) 258, 483
Bsh1236I CGCG 3 cut(s) 105, 353, 528
BsiHKCI CYCGRG 1 cut(s) 150
BsiSI CCGG 3 cut(s) 168, 342, 367
BslI CCNNNNNNNGG 2 cut(s) 157, 373
BsmAI GTCTC 3 cut(s) 208, 301, 503
BsmBI CGTCTC 1 cut(s) 301
BsoBI CYCGRG 1 cut(s) 150
Bsp143I GATC 3 cut(s) 4, 441, 486
BspACI CCGC 3 cut(s) 317, 382, 528
BspCNI CTCAG 2 cut(s) 218, 492
BspFNI CGCG 3 cut(s) 105, 353, 528
BspLI GGNNCC 1 cut(s) 92
BspPI GGATC 1 cut(s) 12
BsrFI RCCGGY 2 cut(s) 167, 341
BssAI RCCGGY 2 cut(s) 167, 341
BssMI GATC 3 cut(s) 4, 441, 486
BssNI GRCGYC 3 cut(s) 165, 294, 345
BssSI CACGAG 1 cut(s) 24
Bst2BI CACGAG 1 cut(s) 24
Bst4CI ACNGT 3 cut(s) 137, 281, 450
BstACI GRCGYC 3 cut(s) 165, 294, 345
BstC8I GCNNGC 1 cut(s) 343
BstDEI CTNAG 2 cut(s) 205, 500
BstF5I GGATG 1 cut(s) 166
BstFNI CGCG 3 cut(s) 105, 353, 528
BstH2I RGCGCY 1 cut(s) 216
BstHHI GCGC 4 cut(s) 107, 215, 355, 520
BstKTI GATC 3 cut(s) 7, 444, 489
BstMAI GTCTC 3 cut(s) 208, 301, 503
BstMBI GATC 3 cut(s) 4, 441, 486
BstMWI GCNNNNNNNGC 2 cut(s) 342, 568
BstUI CGCG 3 cut(s) 105, 353, 528
BstV1I GCAGC 2 cut(s) 258, 483
BstX2I RGATCY 1 cut(s) 4
BstYI RGATCY 1 cut(s) 4
BtsCI GGATG 1 cut(s) 166
BtsIMutI CAGTG 1 cut(s) 294
Cac8I GCNNGC 1 cut(s) 343
CfoI GCGC 4 cut(s) 107, 215, 355, 520
Cfr10I RCCGGY 2 cut(s) 167, 341
CseI GACGC 3 cut(s) 173, 313, 334
CviAII CATG 1 cut(s) 481
CviJI RGCY 6 cut(s) 249, 270, 411, 431, 496, 562
CviKI_1 RGCY 6 cut(s) 249, 270, 411, 431, 496, 562
DdeI CTNAG 2 cut(s) 205, 500
DpnI GATC 3 cut(s) 6, 443, 488
DpnII GATC 3 cut(s) 4, 441, 486
Eco57I CTGAAG 1 cut(s) 270
Eco88I CYCGRG 1 cut(s) 150
Esp3I CGTCTC 1 cut(s) 301
FaeI CATG 1 cut(s) 484
FaiI YATR 4 cut(s) 44, 97, 482, 536
FatI CATG 1 cut(s) 480
Fnu4HI GCNGC 3 cut(s) 247, 497, 529
FokI GGATG 1 cut(s) 173
Fsp4HI GCNGC 3 cut(s) 247, 497, 529
FspBI CTAG 1 cut(s) 252
GlaI GCGC 4 cut(s) 106, 214, 354, 519
GluI GCNGC 3 cut(s) 247, 497, 529
HaeII RGCGCY 1 cut(s) 216
HapII CCGG 3 cut(s) 168, 342, 367
HgaI GACGC 3 cut(s) 173, 313, 334
HhaI GCGC 4 cut(s) 107, 215, 355, 520
Hin1I GRCGYC 3 cut(s) 165, 294, 345
Hin1II CATG 1 cut(s) 484
Hin6I GCGC 4 cut(s) 105, 213, 353, 518
HinP1I GCGC 4 cut(s) 105, 213, 353, 518
HinfI GANTC 1 cut(s) 523
HpaII CCGG 3 cut(s) 168, 342, 367
HphI GGTGA 2 cut(s) 182, 484
Hpy166II GTNNAC 2 cut(s) 292, 475
Hpy188I TCNGA 1 cut(s) 208
Hpy188III TCNNGA 6 cut(s) 12, 140, 265, 322, 454, 576
Hpy8I GTNNAC 2 cut(s) 292, 475
Hpy99I CGWCG 2 cut(s) 137, 307
HpyAV CCTTC 2 cut(s) 203, 543
HpyCH4III ACNGT 3 cut(s) 137, 281, 450
HpyCH4IV ACGT 1 cut(s) 294
HpyCH4V TGCA 1 cut(s) 228
HpyF10VI GCNNNNNNNGC 2 cut(s) 342, 568
HpyF3I CTNAG 2 cut(s) 205, 500
HpySE526I ACGT 1 cut(s) 294
Hsp92I GRCGYC 3 cut(s) 165, 294, 345
Hsp92II CATG 1 cut(s) 484
HspAI GCGC 4 cut(s) 105, 213, 353, 518
KroI GCCGGC 1 cut(s) 341
KroNI GCCGGC 1 cut(s) 343
Kzo9I GATC 3 cut(s) 4, 441, 486
LpnPI CCDG 9 cut(s) 72, 93, 125, 143, 181, 256, 355, 380, 419
Lsp1109I GCAGC 2 cut(s) 258, 483
LweI GCATC 1 cut(s) 558
MaeI CTAG 1 cut(s) 252
MaeII ACGT 1 cut(s) 294
MaeIII GTNAC 1 cut(s) 170
MalI GATC 3 cut(s) 6, 443, 488
MboI GATC 3 cut(s) 4, 441, 486
MboII GAAGA 5 cut(s) 337, 355, 496, 558, 591
MflI RGATCY 1 cut(s) 4
MluCI AATT 3 cut(s) 15, 230, 467
MmeI TCCRAC 1 cut(s) 86
MnlI CCTC 3 cut(s) 33, 359, 533
MreI CGCCGGCG 1 cut(s) 341
MroNI GCCGGC 1 cut(s) 341
MroXI GAANNNNTTC 1 cut(s) 329
MseI TTAA 2 cut(s) 393, 554
MspI CCGG 3 cut(s) 168, 342, 367
MvnI CGCG 3 cut(s) 105, 353, 528
MwoI GCNNNNNNNGC 2 cut(s) 342, 568
NaeI GCCGGC 1 cut(s) 343
NdeII GATC 3 cut(s) 4, 441, 486
NgoMIV GCCGGC 1 cut(s) 341
NlaIII CATG 1 cut(s) 484
NlaIV GGNNCC 1 cut(s) 92
NmuCI GTSAC 1 cut(s) 170
PdiI GCCGGC 1 cut(s) 343
PdmI GAANNNNTTC 1 cut(s) 329
PfeI GAWTC 1 cut(s) 523
PkrI GCNGC 3 cut(s) 248, 498, 530
PspN4I GGNNCC 1 cut(s) 92
PsuI RGATCY 1 cut(s) 4
SaqAI TTAA 2 cut(s) 393, 554
SatI GCNGC 3 cut(s) 247, 497, 529
Sau3AI GATC 3 cut(s) 4, 441, 486
SetI ASST 6 cut(s) 251, 272, 297, 413, 433, 554
SfaNI GCATC 1 cut(s) 558
SgrAI CRCCGGYG 2 cut(s) 167, 341
Sse9I AATT 3 cut(s) 15, 230, 467
SsiI CCGC 3 cut(s) 317, 382, 528
SspMI CTAG 1 cut(s) 252
TaaI ACNGT 3 cut(s) 137, 281, 450
TaiI ACGT 1 cut(s) 297
TaqI TCGA 3 cut(s) 13, 264, 575
TasI AATT 3 cut(s) 15, 230, 467
TauI GCSGC 1 cut(s) 531
TfiI GAWTC 1 cut(s) 523
Tru1I TTAA 2 cut(s) 393, 554
Tru9I TTAA 2 cut(s) 393, 554
TscAI CASTG 1 cut(s) 294
TseFI GTSAC 1 cut(s) 170
TseI GCWGC 2 cut(s) 246, 496
Tsp45I GTSAC 1 cut(s) 170
TspDTI ATGAA 1 cut(s) 497
TspGWI ACGGA 1 cut(s) 419
TspRI CASTG 1 cut(s) 294
XapI RAATTY 2 cut(s) 15, 230
XmnI GAANNNNTTC 1 cut(s) 329
XspI CTAG 1 cut(s) 252
ZraI GACGTC 1 cut(s) 295
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.