RchiOBHm_Chr4g0440971

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0016044
Physical Location & Seq
Forward (+)
62871921 .. 62874087
2167 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40889

Sequence Viewer

Length: 1608 bp
ATGGGTCTCTCTAGATTTTTCAAGCTTTTACCAAAACCATCCAAAAATCCCTTCTTTCTTTTCCCAAATCCTCAAACTGGTTCTCCCAGATCAGTGAGATCACTCACAAACCTTACTAGTAATCCAAACCCCTATGTTGAACACCCATCTGGGCTCTTACGCAATCCCAATCCTGGTATTTACAGAGCAATCCATGGCACTCTATACAAAACCCAAGTGCTTGATGAGGCCAATACCCACGATATCACCCAAGAGGCTGAGAAAATCAGCAAAATTCTATCCACGGCCAACTCCAATCCTCGCATTGAGAGCTTGCTCGACGGCGCTTCAGTCAAGGTGTCACCAACGCTTGTCGTTGAGGTCTTGAAGAAGCTCAACAACGCCGGCGCTCTGGCGCTCTCGTTTTTCCGGTGGGCCGAAAAGCAAAAGGGGTTTAAGCATTGCACAGAGAGCTACAATGCTCTGATTGAAGCTCTGGGTAAGATCAAACAGTTCAAGATAATATGGGAATTGGTAAATGACATGAACAACAAAGGCCTGCTCAGCAAAGAGACTTTCGCTTTGATTTCGAGGCGGTATGCGAGGGCGAGGAAGGTTTCAGAGGGCATTAAAGCTTTTGAGAAGATGGAGAAGTTTGGGATCAAGTTAGAAGGGTCGGATTTCAATAGACTGATTGATACTCTGAGCAAGTCGAGGCAGGTTGAGAAAGCACAGGAGGTGTTCGATGAAATGAAGCACAGAAGGTTCAAACCTGATATTAAGTCGTACACTATTTTGCTGGAAGGATGGGGTCAGCAACAGAATTTTTTGAGGTTGGATGAGGTTTATCGAGAAATGAAGGCTGAAGGGTTCGATCCAGATGTTGTGACTTATGGTATTTTGATCAATGCACATTGTAAGGGCAAGAAGTATAACGAAGCGATTGGATTGTTTCGAGAGATGGAAGCGAAGAATGTGAGGCCTTCTCCTCATATCTTCTGTATGCTGATTAATGGATTAGGCTCAGAAAAGAGATTGGCTGAAGCTCTTGAGTTTTTTGAAGGGTCCAAAGCCAGTGGTTTAGAACCGGAGGCGCCTACTTATAATGCTGTTGTTGGAGCTTACTGCTGGTCAATGCGGATCCCTGACGCGTTCGGGGTGGTGGATGAGATGAGAAAATGTGGGATTGGTCCTAATTCTCGAACCTACGATATAATCTTGCATCACTTGGTTAAGCATCGAAGAACTGAACAAGCTTACTCGGTATTCCAGAAAATCAGTAGCGAACCAACAGTAACTGTAAGTACATACGAAATCCTAGTGAGGATGTTTTGCAATGAGAAGCGAGTGGATATGGCATTACGGGTTTGGGATGAGATGAAGACTAAAGGCGTCCTGCCAGGAATGCACATGTTCTCCACATTGATCAACAGCTTGTGCCATGAGAACAAGTTAGATGATGCATGCAGGTACTTTCAGGAGATGCTAGACATGGGTATTAGGCCTCCGACACAGTTGTTTAGTAACCTAAAGCAAGCGCTTCTTGATGATGGGAGAAAGGATGCTGTCGTAAGTTTCGGTTTGAAGATTGAAAAACTGAGAAAAACCCCATTAGTTGGGTTGCGCTAA

Protein Analysis

535

Amino Acids

60.89

Weight (kDa)

9.32

Isoelectric Point (pI)

33.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_long PF17177 194 - 321 4e-08 Pentacotripeptide-repeat region of PRORP
TPR_24 PF23276 203 - 314 3.6e-06 Fungal tetratrico peptide repeats
PPR_3 PF13812 206 - 264 5.9e-08 Pentatricopeptide repeat domain
PPR_2 PF13041 221 - 262 2.5e-11 PPR repeat family
PPR_3 PF13812 249 - 298 1.4e-06 Pentatricopeptide repeat domain
PPR_2 PF13041 274 - 300 2.8e-07 PPR repeat family
PPR_3 PF13812 275 - 334 1.4e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 283 - 315 1.1e-10 PPR repeat
PPR_2 PF13041 286 - 333 2.8e-12 PPR repeat family
PPR PF01535 289 - 319 3.6e-07 PPR repeat
PPR_long PF17177 304 - 422 6.6e-11 Pentacotripeptide-repeat region of PRORP
PPR_2 PF13041 359 - 405 2.2e-10 PPR repeat family
PPR_2 PF13041 426 - 474 3.5e-11 PPR repeat family
PPR_1 PF12854 426 - 454 1e-05 PPR repeat
PPR_1 PF12854 456 - 488 6.1e-07 PPR repeat
PPR PF01535 464 - 493 8.3e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016044)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G71060
fragaria_vesca FvH4_4g32230
malus_domestica MD13G1027000.v1.1 MD16G1030100.v1.1
prunus_persica Prupe.1G322900_v2.0.a1
pyrus_communis pycom13g02570
rosa_chinensis RchiOBHm_Chr4g0440971
rosa_laevigata RLG00000006135
rosa_multiflora Rmu_sc0000424.1_g000028
rosa_roxburghii Rroxscaffold_5G00381830
rosa_rugosa Rorug04G0329800
rosa_samantha Rh4BG394900 Rh4CG409800 Rh4DG388100
rosa_wichuraiana Rw4G032870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1083
Acc36I ACCTGC 2 cut(s) 688, 1437
AccB1I GGYRCC 1 cut(s) 1072
AccB7I CCANNNNNTGG 1 cut(s) 1595
AccII CGCG 1 cut(s) 1130
AciI CCGC 2 cut(s) 574, 1117
AclWI GGATC 4 cut(s) 647, 848, 1114, 1127
AcoI YGGCCR 1 cut(s) 285
AcsI RAATTY 2 cut(s) 273, 802
AcuI CTGAAG 3 cut(s) 312, 864, 1041
AcyI GRCGYC 2 cut(s) 1073, 1371
AfaI GTAC 3 cut(s) 767, 1285, 1451
AfeI AGCGCT 1 cut(s) 1518
AfiI CCNNNNNNNGG 3 cut(s) 77, 173, 1595
AflIII ACRYGT 2 cut(s) 1128, 1389
AhlI ACTAGT 1 cut(s) 116
AjnI CCWGG 2 cut(s) 172, 1378
AjuI GAANNNNNNNTTGG 2 cut(s) 35, 67
Alw26I GTCTC 2 cut(s) 11, 545
AlwI GGATC 4 cut(s) 647, 848, 1114, 1127
AlwNI CAGNNNCTG 1 cut(s) 1277
Aor51HI AGCGCT 1 cut(s) 1518
AoxI GGCC 6 cut(s) 228, 285, 414, 535, 959, 1481
ApoI RAATTY 2 cut(s) 273, 802
ArsI GACNNNNNNTTYG 2 cut(s) 544, 576
AseI ATTAAT 1 cut(s) 990
AspLEI GCGC 6 cut(s) 326, 389, 397, 1075, 1519, 1605
AspS9I GGNCC 3 cut(s) 414, 1044, 1169
AsuHPI GGTGA 2 cut(s) 238, 333
AvaII GGWCC 2 cut(s) 1044, 1169
BamHI GGATCC 1 cut(s) 1119
BanI GGYRCC 1 cut(s) 1072
BanII GRGCYC 1 cut(s) 156
BbsI GAAGAC 1 cut(s) 1367
BccI CCATC 6 cut(s) 46, 154, 619, 780, 934, 1523
BceAI ACGGC 2 cut(s) 300, 337
BciT130I CCWGG 2 cut(s) 174, 1380
BclI TGATCA 2 cut(s) 882, 1404
BcoDI GTCTC 2 cut(s) 11, 545
BcuI ACTAGT 1 cut(s) 116
BfaI CTAG 4 cut(s) 12, 117, 1298, 1466
BfoI RGCGCY 5 cut(s) 327, 390, 398, 1076, 1520
BfuAI ACCTGC 2 cut(s) 688, 1437
BlpI GCTNAGC 1 cut(s) 542
Bme1390I CCNGG 2 cut(s) 174, 1380
Bme18I GGWCC 2 cut(s) 1044, 1169
BmgT120I GGNCC 3 cut(s) 414, 1044, 1169
BmiI GGNNCC 3 cut(s) 1045, 1074, 1121
BmrFI CCNGG 2 cut(s) 174, 1380
BmsI GCATC 5 cut(s) 1210, 1225, 1429, 1452, 1531
BpiI GAAGAC 1 cut(s) 1367
BplI GAGNNNNNCTC 2 cut(s) 949, 981
Bpu1102I GCTNAGC 1 cut(s) 542
BpuEI CTTGAG 1 cut(s) 1049
BsaHI GRCGYC 2 cut(s) 1073, 1371
BsaI GGTCTC 1 cut(s) 11
BsaJI CCNNGG 2 cut(s) 193, 282
BsaWI WCCGGW 2 cut(s) 408, 1066
BsaXI ACNNNNNCTCC 4 cut(s) 67, 97, 1379, 1409
Bsc4I CCNNNNNNNGG 3 cut(s) 77, 173, 1595
Bse118I RCCGGY 1 cut(s) 383
Bse1I ACTGG 2 cut(s) 82, 1053
Bse3DI GCAATG 2 cut(s) 439, 1321
BseBI CCWGG 2 cut(s) 174, 1380
BseDI CCNNGG 2 cut(s) 193, 282
BseGI GGATG 7 cut(s) 38, 791, 823, 1150, 1311, 1357, 1546
BseLI CCNNNNNNNGG 3 cut(s) 77, 173, 1595
BseMI GCAATG 2 cut(s) 439, 1321
BseMII CTCAG 5 cut(s) 249, 556, 674, 1017, 1568
BseNI ACTGG 2 cut(s) 82, 1053
BseRI GAGGAG 1 cut(s) 957
Bsh1236I CGCG 1 cut(s) 1130
BshFI GGCC 6 cut(s) 230, 287, 416, 537, 961, 1483
BshNI GGYRCC 1 cut(s) 1072
BsiSI CCGG 3 cut(s) 384, 409, 1067
BslI CCNNNNNNNGG 3 cut(s) 77, 173, 1595
BsmAI GTCTC 2 cut(s) 11, 545
BsmI GAATGC 1 cut(s) 1389
BsnI GGCC 6 cut(s) 230, 287, 416, 537, 961, 1483
Bso31I GGTCTC 1 cut(s) 11
Bsp1286I GDGCHC 1 cut(s) 156
Bsp143I GATC 8 cut(s) 89, 98, 483, 639, 853, 882, 1119, 1404
Bsp1720I GCTNAGC 1 cut(s) 542
Bsp19I CCATGG 1 cut(s) 193
BspACI CCGC 2 cut(s) 574, 1117
BspANI GGCC 6 cut(s) 230, 287, 416, 537, 961, 1483
BspCNI CTCAG 5 cut(s) 250, 555, 675, 1016, 1569
BspFNI CGCG 1 cut(s) 1130
BspLI GGNNCC 3 cut(s) 1045, 1074, 1121
BspMI ACCTGC 2 cut(s) 688, 1437
BspPI GGATC 4 cut(s) 647, 848, 1114, 1127
BspT107I GGYRCC 1 cut(s) 1072
BspTNI GGTCTC 1 cut(s) 11
BsrDI GCAATG 2 cut(s) 439, 1321
BsrFI RCCGGY 1 cut(s) 383
BsrI ACTGG 2 cut(s) 82, 1053
BssAI RCCGGY 1 cut(s) 383
BssECI CCNNGG 2 cut(s) 193, 282
BssMI GATC 8 cut(s) 89, 98, 483, 639, 853, 882, 1119, 1404
BssNI GRCGYC 2 cut(s) 1073, 1371
BssT1I CCWWGG 1 cut(s) 193
Bst2UI CCWGG 2 cut(s) 174, 1380
Bst4CI ACNGT 4 cut(s) 492, 1273, 1279, 1494
BstACI GRCGYC 2 cut(s) 1073, 1371
BstC8I GCNNGC 5 cut(s) 314, 385, 539, 1444, 1515
BstDEI CTNAG 5 cut(s) 258, 542, 683, 1003, 1577
BstDSI CCRYGG 2 cut(s) 193, 282
BstF5I GGATG 7 cut(s) 38, 791, 823, 1150, 1311, 1357, 1546
BstFNI CGCG 1 cut(s) 1130
BstH2I RGCGCY 5 cut(s) 327, 390, 398, 1076, 1520
BstHHI GCGC 6 cut(s) 326, 389, 397, 1075, 1519, 1605
BstKTI GATC 8 cut(s) 92, 101, 486, 642, 856, 885, 1122, 1407
BstMAI GTCTC 2 cut(s) 11, 545
BstMBI GATC 8 cut(s) 89, 98, 483, 639, 853, 882, 1119, 1404
BstMWI GCNNNNNNNGC 4 cut(s) 309, 450, 543, 1384
BstNI CCWGG 2 cut(s) 174, 1380
BstNSI RCATGY 2 cut(s) 1393, 1446
BstSCI CCNGG 2 cut(s) 172, 1378
BstUI CGCG 1 cut(s) 1130
BstV2I GAAGAC 1 cut(s) 1367
BstX2I RGATCY 1 cut(s) 1119
BstYI RGATCY 1 cut(s) 1119
BsuRI GGCC 6 cut(s) 230, 287, 416, 537, 961, 1483
BtgI CCRYGG 2 cut(s) 193, 282
BtsCI GGATG 7 cut(s) 38, 791, 823, 1150, 1311, 1357, 1546
BtsIMutI CAGTG 2 cut(s) 99, 1060
BveI ACCTGC 2 cut(s) 688, 1437
Cac8I GCNNGC 5 cut(s) 314, 385, 539, 1444, 1515
CaiI CAGNNNCTG 1 cut(s) 1277
CfoI GCGC 6 cut(s) 326, 389, 397, 1075, 1519, 1605
Cfr10I RCCGGY 1 cut(s) 383
Cfr13I GGNCC 3 cut(s) 414, 1044, 1169
CseI GACGC 2 cut(s) 1136, 1360
Csp6I GTAC 3 cut(s) 766, 1284, 1450
CspCI CAANNNNNGTGG 2 cut(s) 1036, 1071
CviAII CATG 6 cut(s) 194, 523, 1390, 1421, 1443, 1471
CviQI GTAC 3 cut(s) 766, 1284, 1450
DdeI CTNAG 5 cut(s) 258, 542, 683, 1003, 1577
DinI GGCGCC 1 cut(s) 1074
DpnI GATC 8 cut(s) 91, 100, 485, 641, 855, 884, 1121, 1406
DpnII GATC 8 cut(s) 89, 98, 483, 639, 853, 882, 1119, 1404
EaeI YGGCCR 1 cut(s) 285
Eco130I CCWWGG 1 cut(s) 193
Eco147I AGGCCT 3 cut(s) 537, 961, 1483
Eco24I GRGCYC 1 cut(s) 156
Eco31I GGTCTC 1 cut(s) 11
Eco32I GATATC 1 cut(s) 244
Eco47I GGWCC 2 cut(s) 1044, 1169
Eco47III AGCGCT 1 cut(s) 1518
Eco57I CTGAAG 3 cut(s) 312, 864, 1041
EcoRII CCWGG 2 cut(s) 172, 1378
EcoRV GATATC 1 cut(s) 244
EcoT14I CCWWGG 1 cut(s) 193
EcoT22I ATGCAT 1 cut(s) 1444
EcoT38I GRGCYC 1 cut(s) 156
EgeI GGCGCC 1 cut(s) 1074
EheI GGCGCC 1 cut(s) 1074
ErhI CCWWGG 1 cut(s) 193
FaeI CATG 6 cut(s) 197, 526, 1393, 1424, 1446, 1474
FalI AAGNNNNNCTT 2 cut(s) 1506, 1538
FatI CATG 6 cut(s) 193, 522, 1389, 1420, 1442, 1470
FbaI TGATCA 2 cut(s) 882, 1404
FokI GGATG 7 cut(s) 25, 798, 830, 1157, 1318, 1364, 1553
FriOI GRGCYC 1 cut(s) 156
FspBI CTAG 4 cut(s) 12, 117, 1298, 1466
GlaI GCGC 6 cut(s) 325, 388, 396, 1074, 1518, 1604
HaeII RGCGCY 5 cut(s) 327, 390, 398, 1076, 1520
HaeIII GGCC 6 cut(s) 230, 287, 416, 537, 961, 1483
HapII CCGG 3 cut(s) 384, 409, 1067
HgaI GACGC 2 cut(s) 1136, 1360
HhaI GCGC 6 cut(s) 326, 389, 397, 1075, 1519, 1605
Hin1I GRCGYC 2 cut(s) 1073, 1371
Hin1II CATG 6 cut(s) 197, 526, 1393, 1424, 1446, 1474
Hin6I GCGC 6 cut(s) 324, 387, 395, 1073, 1517, 1603
HinP1I GCGC 6 cut(s) 324, 387, 395, 1073, 1517, 1603
HindIII AAGCTT 3 cut(s) 23, 612, 1233
HpaII CCGG 3 cut(s) 384, 409, 1067
HphI GGTGA 2 cut(s) 238, 333
Hpy166II GTNNAC 1 cut(s) 768
Hpy188I TCNGA 6 cut(s) 465, 601, 658, 684, 1006, 1488
Hpy8I GTNNAC 1 cut(s) 768
Hpy99I CGWCG 1 cut(s) 323
HpyAV CCTTC 9 cut(s) 61, 586, 644, 735, 776, 832, 839, 972, 1034
HpyCH4III ACNGT 4 cut(s) 492, 1273, 1279, 1494
HpyCH4V TGCA 7 cut(s) 444, 890, 1201, 1314, 1387, 1442, 1446
HpyF10VI GCNNNNNNNGC 4 cut(s) 309, 450, 543, 1384
HpyF3I CTNAG 5 cut(s) 258, 542, 683, 1003, 1577
Hsp92I GRCGYC 2 cut(s) 1073, 1371
Hsp92II CATG 6 cut(s) 197, 526, 1393, 1424, 1446, 1474
HspAI GCGC 6 cut(s) 324, 387, 395, 1073, 1517, 1603
KasI GGCGCC 1 cut(s) 1072
KroI GCCGGC 1 cut(s) 383
KroNI GCCGGC 1 cut(s) 385
Ksp22I TGATCA 2 cut(s) 882, 1404
Kzo9I GATC 8 cut(s) 89, 98, 483, 639, 853, 882, 1119, 1404
LmnI GCTCC 1 cut(s) 1097
LweI GCATC 5 cut(s) 1210, 1225, 1429, 1452, 1531
MaeI CTAG 4 cut(s) 12, 117, 1298, 1466
MaeIII GTNAC 4 cut(s) 339, 865, 1273, 1502
MalI GATC 8 cut(s) 91, 100, 485, 641, 855, 884, 1121, 1406
MboI GATC 8 cut(s) 89, 98, 483, 639, 853, 882, 1119, 1404
MboII GAAGA 7 cut(s) 379, 634, 961, 967, 1233, 1372, 1576
MflI RGATCY 1 cut(s) 1119
MhlI GDGCHC 1 cut(s) 156
MluCI AATT 4 cut(s) 273, 509, 802, 1174
MluI ACGCGT 1 cut(s) 1128
Mly113I GGCGCC 1 cut(s) 1073
MmeI TCCRAC 4 cut(s) 636, 795, 1075, 1511
Mph1103I ATGCAT 1 cut(s) 1444
MreI CGCCGGCG 1 cut(s) 383
MroNI GCCGGC 1 cut(s) 383
MseI TTAA 5 cut(s) 435, 609, 759, 990, 1212
MspI CCGG 3 cut(s) 384, 409, 1067
MspR9I CCNGG 2 cut(s) 174, 1380
Mva1269I GAATGC 1 cut(s) 1389
MvaI CCWGG 2 cut(s) 174, 1380
MvnI CGCG 1 cut(s) 1130
MwoI GCNNNNNNNGC 4 cut(s) 309, 450, 543, 1384
NaeI GCCGGC 1 cut(s) 385
NarI GGCGCC 1 cut(s) 1073
NcoI CCATGG 1 cut(s) 193
NdeII GATC 8 cut(s) 89, 98, 483, 639, 853, 882, 1119, 1404
NgoMIV GCCGGC 1 cut(s) 383
NlaIII CATG 6 cut(s) 197, 526, 1393, 1424, 1446, 1474
NlaIV GGNNCC 3 cut(s) 1045, 1074, 1121
NmuCI GTSAC 2 cut(s) 339, 865
NsiI ATGCAT 1 cut(s) 1444
NspI RCATGY 2 cut(s) 1393, 1446
PaeI GCATGC 1 cut(s) 1446
PceI AGGCCT 3 cut(s) 537, 961, 1483
PciI ACATGT 1 cut(s) 1389
PctI GAATGC 1 cut(s) 1389
PdiI GCCGGC 1 cut(s) 385
PflMI CCANNNNNTGG 1 cut(s) 1595
PluTI GGCGCC 1 cut(s) 1076
PscI ACATGT 1 cut(s) 1389
PshBI ATTAAT 1 cut(s) 990
PsiI TTATAA 1 cut(s) 1083
Psp6I CCWGG 2 cut(s) 172, 1378
PspGI CCWGG 2 cut(s) 172, 1378
PspN4I GGNNCC 3 cut(s) 1045, 1074, 1121
PspPI GGNCC 3 cut(s) 414, 1044, 1169
PstNI CAGNNNCTG 1 cut(s) 1277
PsuI RGATCY 1 cut(s) 1119
RsaI GTAC 3 cut(s) 767, 1285, 1451
RsaNI GTAC 3 cut(s) 766, 1284, 1450
SaqAI TTAA 5 cut(s) 435, 609, 759, 990, 1212
Sau3AI GATC 8 cut(s) 89, 98, 483, 639, 853, 882, 1119, 1404
Sau96I GGNCC 3 cut(s) 414, 1044, 1169
ScrFI CCNGG 2 cut(s) 174, 1380
SduI GDGCHC 1 cut(s) 156
SfaNI GCATC 5 cut(s) 1210, 1225, 1429, 1452, 1531
SfoI GGCGCC 1 cut(s) 1074
SgrAI CRCCGGYG 1 cut(s) 383
SinI GGWCC 2 cut(s) 1044, 1169
SmlI CTYRAG 1 cut(s) 1028
SmoI CTYRAG 1 cut(s) 1028
SpeI ACTAGT 1 cut(s) 116
SphI GCATGC 1 cut(s) 1446
Sse9I AATT 4 cut(s) 273, 509, 802, 1174
SseBI AGGCCT 3 cut(s) 537, 961, 1483
SsiI CCGC 2 cut(s) 574, 1117
SspDI GGCGCC 1 cut(s) 1072
SspMI CTAG 4 cut(s) 12, 117, 1298, 1466
StuI AGGCCT 3 cut(s) 537, 961, 1483
StyD4I CCNGG 2 cut(s) 172, 1378
StyI CCWWGG 1 cut(s) 193
TaaI ACNGT 4 cut(s) 492, 1273, 1279, 1494
TaqI TCGA 9 cut(s) 318, 569, 692, 723, 829, 852, 934, 1180, 1219
TasI AATT 4 cut(s) 273, 509, 802, 1174
TatI WGTACW 1 cut(s) 1283
Tru1I TTAA 5 cut(s) 435, 609, 759, 990, 1212
Tru9I TTAA 5 cut(s) 435, 609, 759, 990, 1212
TscAI CASTG 2 cut(s) 99, 1060
TseFI GTSAC 2 cut(s) 339, 865
Tsp45I GTSAC 2 cut(s) 339, 865
TspDTI ATGAA 5 cut(s) 539, 741, 746, 851, 1373
TspRI CASTG 2 cut(s) 99, 1060
Van91I CCANNNNNTGG 1 cut(s) 1595
VpaK11BI GGWCC 2 cut(s) 1044, 1169
VspI ATTAAT 1 cut(s) 990
XapI RAATTY 2 cut(s) 273, 802
XbaI TCTAGA 1 cut(s) 11
XceI RCATGY 2 cut(s) 1393, 1446
XspI CTAG 4 cut(s) 12, 117, 1298, 1466
Zsp2I ATGCAT 1 cut(s) 1444
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.