Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0011104

Physical Location & Seq

Genomic Coordinates Forward (+)

5944316 .. 5947861

Gene Structure 3546 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1084800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1203 bp

ATGGCTCTATCCCGCCTAGCCTCCTCCTCCTCTGGCTCCAATCTCCTGAGGCCCTTCGCCTCCGCCTTATCCCTCACCCCATCTCTCCGCCGTCCGATCTCATCCGCCGCCGATGACTCCACCACCCTCACGTTCGAGACCTCCCTCCCCTTCACCTCCCACAACTGTGAGCCGCCGTCGCGCACGCTCGAGACCACTCCCAAGGAGCTTATGACGTTCTTCCGCGACATGGCGTTGATGCGCCGGATGGAGATCGCAGCCGACTCGCTCTACAAGGCCAAGCTGATCCGAGGGTTTTGCCACCTATACGACGGCCAGGAGGCCGTCGCCATCGGCATGGAGGCCGCGATTACCAAAAAGGACTGCATCATCACTGCGTACCGTGACCACTGCACCTTCCTCGGCCGCGGTGGGACCCTCCTGCAGGTTTTCGCCGAGCTCATGGGGCGCCAGGCTGGCTGCTCCAAGGGGAAAGGTGGGTCCATGCATTTCTACAAGAAGGACGCTGGATTCTATGGTGGCCACGGTATAGTCGGCGCTCAGATTCCGCTGGGATGTGGATTGGCTTTTGGGCAAAAGTACTCCAAGGACGAAACGGTGACGTTTGCTCTCTACGGTGACGGTGCAGCCAATCAAGGGCAGTTGTTCGAGGCGCTTAACATTTCTGCGCTTTGGGATCTGCCTGCGATTTTGGTCTGTGAGAATAATCACTATGGAATGGGTACCGCCGAGTGGAGAGCAGCGAAGAGTCCCGCATATTACAAGCGTGGAGATTATGTTCCTGGCTTGAAGGTGGATGGTATGGATGTGTTTGCCGTAAAGCAGGCATGCAAATTTGCCAAGGAGCATGCTCTGAAGAACGGACCCATTATTCTTGAAATGGACACTTACAGGTACCATGGTCACTCTATGTCTGATCCTGGTAGCACCTACCGCACACGTGATGAGATTTCTGGCGTGAGACAGGAGCGTGATCCAATTGATAGAATAAGAAAGCTGATATTTGCTCATGATCTAGCTACTGAGAAGGATCTAAAGGATATCGAGAAGGAAGTAAGAAAAGAAGTTGATGAAGCCATTGCTCAAGCCAAAGAAAGCCCGATGCCAGAACCCTCCGAACTCTTCACAAATATCTATGTGAAAGGCTATGGAACTGAGTCTTTTGGACCAGATAGGAAAGAAATCAGAACTGTACTTCCCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005759 GO:0005829 GO:0006950 GO:0006970 GO:0008150 GO:0008270 GO:0009628 GO:0009651 GO:0010035 GO:0010038 GO:0031974 GO:0042221 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0046872 GO:0046914 GO:0050896 GO:0050897 GO:0070013

KEGG Pathways

Metabolic & Signaling

ko00010 ko00020 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko04066 ko04922 ko05230 map00010 map00020 map00620 map01100 map01110 map01120 map01130 map01200 map04066 map04922 map05230

Pfam Domains

Protein Families

E1_dh

Protein Analysis

401

Amino Acids

44.15

Weight (kDa)

7.13

Isoelectric Point (pI)

41.78

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
E1_dh	PF00676	75 - 371	1.2e-120	Dehydrogenase E1 component

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011104)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G24180 AT1G59900 AT1G59900
fragaria_vesca	FvH4_4g35280
malus_domestica	MD13G1084800.v1.1 MD16G1083900.v1.1
prunus_persica	Prupe.1G262100_v2.0.a1
pyrus_communis	pycom13g07330 pycom16g07290
rosa_chinensis	RchiOBHm_Chr4g0444881
rosa_laevigata	RLG00000005792
rosa_multiflora	Rmu_co8512837.1_g000001 Rmu_sc0006845.1_g000004
rosa_roxburghii	Rroxscaffold_5G00385370
rosa_rugosa	Rorug04G0355800
rosa_samantha	Rh4AG417500 Rh4BG429300 Rh4CG444000 Rh4DG425200
rosa_wichuraiana	Rw4G035780

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	1 cut(s)	415
Acc65I	GGTACC	2 cut(s)	722, 894
AccB1I	GGYRCC	3 cut(s)	447, 722, 894
AccII	CGCG	4 cut(s)	181, 225, 347, 408
AclWI	GGATC	5 cut(s)	280, 684, 911, 968, 1038
AcoI	YGGCCR	3 cut(s)	313, 403, 520
AcsI	RAATTY	1 cut(s)	833
AcuI	CTGAAG	1 cut(s)	875
AcvI	CACGTG	1 cut(s)	941
AcyI	GRCGYC	1 cut(s)	448
AfaI	GTAC	5 cut(s)	380, 581, 724, 896, 1194
AfiI	CCNNNNNNNGG	4 cut(s)	229, 424, 636, 732
AflIII	ACRYGT	1 cut(s)	938
AgsI	TTSAA	2 cut(s)	790, 878
AjnI	CCWGG	4 cut(s)	315, 450, 781, 919
AleI	CACNNNNGTG	1 cut(s)	165
AloI	GAACNNNNNNTCC	3 cut(s)	762, 794, 1180
AluBI	AGCT	5 cut(s)	208, 283, 439, 997, 1019
AluI	AGCT	5 cut(s)	208, 283, 439, 997, 1019
Alw21I	GWGCWC	1 cut(s)	441
Alw26I	GTCTC	3 cut(s)	131, 185, 955
AlwI	GGATC	5 cut(s)	280, 684, 911, 968, 1038
Ama87I	CYCGRG	1 cut(s)	188
AoxI	GGCC	7 cut(s)	50, 276, 313, 321, 342, 403, 520
ApeKI	GCWGC	4 cut(s)	257, 459, 626, 740
ApoI	RAATTY	1 cut(s)	833
Asp718I	GGTACC	2 cut(s)	722, 894
AspLEI	GCGC	6 cut(s)	183, 243, 450, 539, 655, 670
AspS9I	GGNCC	5 cut(s)	51, 414, 480, 863, 1166
AsuHPI	GGTGA	4 cut(s)	67, 145, 610, 629
AvaI	CYCGRG	1 cut(s)	188
AvaII	GGWCC	4 cut(s)	414, 480, 863, 1166
AxyI	CCTNAGG	1 cut(s)	47
BaeI	ACNNNNGTAYC	2 cut(s)	886, 919
BalI	TGGCCA	1 cut(s)	522
BanI	GGYRCC	3 cut(s)	447, 722, 894
BanII	GRGCYC	1 cut(s)	441
BbrPI	CACGTG	1 cut(s)	941
Bbv12I	GWGCWC	1 cut(s)	441
BbvI	GCAGC	4 cut(s)	269, 446, 638, 752
BccI	CCATC	4 cut(s)	88, 241, 338, 791
BceAI	ACGGC	5 cut(s)	75, 160, 308, 328, 800
BcgI	CGANNNNNNTGC	6 cut(s)	246, 279, 280, 313, 382, 416
BciT130I	CCWGG	4 cut(s)	317, 452, 783, 921
BcoDI	GTCTC	3 cut(s)	131, 185, 955
BfaI	CTAG	2 cut(s)	17, 1016
BfmI	CTRYAG	1 cut(s)	422
BfoI	RGCGCY	3 cut(s)	451, 540, 656
BfuAI	ACCTGC	1 cut(s)	415
BglI	GCCNNNNNGGC	1 cut(s)	456
BisI	GCNGC	8 cut(s)	108, 173, 258, 345, 406, 460, 627, 741
BlsI	GCNGC	8 cut(s)	109, 174, 259, 346, 407, 461, 628, 742
BmcAI	AGTACT	1 cut(s)	581
Bme1390I	CCNGG	4 cut(s)	317, 452, 783, 921
Bme18I	GGWCC	4 cut(s)	414, 480, 863, 1166
BmeT110I	CYCGRG	1 cut(s)	188
BmgT120I	GGNCC	5 cut(s)	51, 414, 480, 863, 1166
BmiI	GGNNCC	8 cut(s)	37, 415, 416, 449, 481, 724, 865, 896
BmrFI	CCNGG	4 cut(s)	317, 452, 783, 921
BmsI	GCATC	3 cut(s)	228, 375, 1092
BpuEI	CTTGAG	1 cut(s)	1068
BsaAI	YACGTR	1 cut(s)	941
BsaBI	GATNNNNATC	1 cut(s)	251
BsaHI	GRCGYC	1 cut(s)	448
BsaI	GGTCTC	2 cut(s)	131, 185
BsaJI	CCNNGG	9 cut(s)	201, 289, 400, 406, 465, 523, 585, 840, 898
BsaXI	ACNNNNNCTCC	2 cut(s)	762, 792
Bsc4I	CCNNNNNNNGG	4 cut(s)	229, 424, 636, 732
Bse21I	CCTNAGG	1 cut(s)	47
Bse3DI	GCAATG	1 cut(s)	1077
Bse8I	GATNNNNATC	1 cut(s)	251
BseBI	CCWGG	4 cut(s)	317, 452, 783, 921
BseDI	CCNNGG	9 cut(s)	201, 289, 400, 406, 465, 523, 585, 840, 898
BseGI	GGATG	5 cut(s)	101, 252, 560, 802, 811
BseJI	GATNNNNATC	1 cut(s)	251
BseLI	CCNNNNNNNGG	4 cut(s)	229, 424, 636, 732
BseMI	GCAATG	1 cut(s)	1077
BseMII	CTCAG	4 cut(s)	38, 554, 1014, 1146
BseRI	GAGGAG	3 cut(s)	13, 16, 19
BseX3I	CGGCCG	1 cut(s)	403
BseXI	GCAGC	4 cut(s)	269, 446, 638, 752
BseYI	CCCAGC	1 cut(s)	550
BsgI	GTGCAG	2 cut(s)	376, 645
Bsh1236I	CGCG	4 cut(s)	181, 225, 347, 408
Bsh1285I	CGRYCG	1 cut(s)	406
BshFI	GGCC	7 cut(s)	52, 278, 315, 323, 344, 405, 522
BshNI	GGYRCC	3 cut(s)	447, 722, 894
BsiEI	CGRYCG	1 cut(s)	406
BsiHKAI	GWGCWC	1 cut(s)	441
BsiHKCI	CYCGRG	1 cut(s)	188
BsiSI	CCGG	1 cut(s)	244
BslFI	GGGAC	2 cut(s)	427, 735
BslI	CCNNNNNNNGG	4 cut(s)	229, 424, 636, 732
BsmAI	GTCTC	3 cut(s)	131, 185, 955
BsmFI	GGGAC	2 cut(s)	427, 735
BsnI	GGCC	7 cut(s)	52, 278, 315, 323, 344, 405, 522
Bso31I	GGTCTC	2 cut(s)	131, 185
BsoBI	CYCGRG	1 cut(s)	188
Bsp1286I	GDGCHC	1 cut(s)	441
Bsp143I	GATC	8 cut(s)	96, 252, 285, 676, 916, 973, 1012, 1030
Bsp19I	CCATGG	1 cut(s)	898
BspANI	GGCC	7 cut(s)	52, 278, 315, 323, 344, 405, 522
BspCNI	CTCAG	4 cut(s)	39, 553, 1015, 1147
BspFNI	CGCG	4 cut(s)	181, 225, 347, 408
BspHI	TCATGA	1 cut(s)	1009
BspLI	GGNNCC	8 cut(s)	37, 415, 416, 449, 481, 724, 865, 896
BspMAI	CTGCAG	1 cut(s)	426
BspMI	ACCTGC	1 cut(s)	415
BspPI	GGATC	5 cut(s)	280, 684, 911, 968, 1038
BspT107I	GGYRCC	3 cut(s)	447, 722, 894
BspTNI	GGTCTC	2 cut(s)	131, 185
BsrDI	GCAATG	1 cut(s)	1077
BssECI	CCNNGG	9 cut(s)	201, 289, 400, 406, 465, 523, 585, 840, 898
BssMI	GATC	8 cut(s)	96, 252, 285, 676, 916, 973, 1012, 1030
BssNI	GRCGYC	1 cut(s)	448
BssT1I	CCWWGG	5 cut(s)	201, 465, 585, 840, 898
Bst2UI	CCWGG	4 cut(s)	317, 452, 783, 921
Bst4CI	ACNGT	7 cut(s)	167, 383, 527, 598, 617, 623, 1192
Bst6I	CTCTTC	2 cut(s)	740, 1127
BstACI	GRCGYC	1 cut(s)	448
BstBAI	YACGTR	1 cut(s)	941
BstC8I	GCNNGC	6 cut(s)	185, 457, 684, 825, 829, 849
BstDEI	CTNAG	4 cut(s)	47, 540, 1023, 1155
BstDSI	CCRYGG	3 cut(s)	406, 523, 898
BstENI	CCTNNNNNAGG	1 cut(s)	422
BstF5I	GGATG	5 cut(s)	101, 252, 560, 802, 811
BstFNI	CGCG	4 cut(s)	181, 225, 347, 408
BstH2I	RGCGCY	3 cut(s)	451, 540, 656
BstHHI	GCGC	6 cut(s)	183, 243, 450, 539, 655, 670
BstKTI	GATC	8 cut(s)	99, 255, 288, 679, 919, 976, 1015, 1033
BstMAI	GTCTC	3 cut(s)	131, 185, 955
BstMBI	GATC	8 cut(s)	96, 252, 285, 676, 916, 973, 1012, 1030
BstMCI	CGRYCG	1 cut(s)	406
BstMWI	GCNNNNNNNGC	4 cut(s)	178, 445, 456, 933
BstNI	CCWGG	4 cut(s)	317, 452, 783, 921
BstNSI	RCATGY	2 cut(s)	831, 851
BstSCI	CCNGG	4 cut(s)	315, 450, 781, 919
BstSFI	CTRYAG	1 cut(s)	422
BstUI	CGCG	4 cut(s)	181, 225, 347, 408
BstV1I	GCAGC	4 cut(s)	269, 446, 638, 752
BstX2I	RGATCY	2 cut(s)	676, 1030
BstXI	CCANNNNNNTGG	1 cut(s)	337
BstYI	RGATCY	2 cut(s)	676, 1030
BstZI	CGGCCG	1 cut(s)	403
Bsu36I	CCTNAGG	1 cut(s)	47
BsuRI	GGCC	7 cut(s)	52, 278, 315, 323, 344, 405, 522
BtgI	CCRYGG	3 cut(s)	406, 523, 898
BtsCI	GGATG	5 cut(s)	101, 252, 560, 802, 811
BtsI	GCAGTG	2 cut(s)	372, 388
BtsIMutI	CAGTG	2 cut(s)	372, 388
BveI	ACCTGC	1 cut(s)	415
Cac8I	GCNNGC	6 cut(s)	185, 457, 684, 825, 829, 849
CciI	TCATGA	1 cut(s)	1009
CfoI	GCGC	6 cut(s)	183, 243, 450, 539, 655, 670
Cfr13I	GGNCC	5 cut(s)	51, 414, 480, 863, 1166
Cfr42I	CCGCGG	1 cut(s)	409
CseI	GACGC	1 cut(s)	512
Csp6I	GTAC	5 cut(s)	379, 580, 723, 895, 1193
CviAII	CATG	8 cut(s)	229, 337, 442, 484, 828, 848, 899, 1010
CviQI	GTAC	5 cut(s)	379, 580, 723, 895, 1193
DdeI	CTNAG	4 cut(s)	47, 540, 1023, 1155
DinI	GGCGCC	1 cut(s)	449
DpnI	GATC	8 cut(s)	98, 254, 287, 678, 918, 975, 1014, 1032
DpnII	GATC	8 cut(s)	96, 252, 285, 676, 916, 973, 1012, 1030
EaeI	YGGCCR	3 cut(s)	313, 403, 520
EagI	CGGCCG	1 cut(s)	403
Eam1104I	CTCTTC	2 cut(s)	740, 1127
EarI	CTCTTC	2 cut(s)	740, 1127
EciI	GGCGGA	3 cut(s)	52, 77, 94
Ecl136II	GAGCTC	1 cut(s)	439
EclXI	CGGCCG	1 cut(s)	403
Eco130I	CCWWGG	5 cut(s)	201, 465, 585, 840, 898
Eco24I	GRGCYC	1 cut(s)	441
Eco31I	GGTCTC	2 cut(s)	131, 185
Eco32I	GATATC	1 cut(s)	1042
Eco47I	GGWCC	4 cut(s)	414, 480, 863, 1166
Eco52I	CGGCCG	1 cut(s)	403
Eco53kI	GAGCTC	1 cut(s)	439
Eco57I	CTGAAG	1 cut(s)	875
Eco72I	CACGTG	1 cut(s)	941
Eco81I	CCTNAGG	1 cut(s)	47
Eco88I	CYCGRG	1 cut(s)	188
EcoICRI	GAGCTC	1 cut(s)	439
EcoNI	CCTNNNNNAGG	1 cut(s)	422
EcoO109I	RGGNCCY	2 cut(s)	51, 414
EcoRII	CCWGG	4 cut(s)	315, 450, 781, 919
EcoRV	GATATC	1 cut(s)	1042
EcoT14I	CCWWGG	5 cut(s)	201, 465, 585, 840, 898
EcoT22I	ATGCAT	1 cut(s)	489
EcoT38I	GRGCYC	1 cut(s)	441
EgeI	GGCGCC	1 cut(s)	449
EheI	GGCGCC	1 cut(s)	449
ErhI	CCWWGG	5 cut(s)	201, 465, 585, 840, 898
FaeI	CATG	8 cut(s)	232, 340, 445, 487, 831, 851, 902, 1013
FaqI	GGGAC	2 cut(s)	427, 735
FatI	CATG	8 cut(s)	228, 336, 441, 483, 827, 847, 898, 1009
FauI	CCCGC	2 cut(s)	20, 760
Fnu4HI	GCNGC	8 cut(s)	108, 173, 258, 345, 406, 460, 627, 741
FokI	GGATG	5 cut(s)	88, 259, 567, 809, 818
FriOI	GRGCYC	1 cut(s)	441
Fsp4HI	GCNGC	8 cut(s)	108, 173, 258, 345, 406, 460, 627, 741
FspBI	CTAG	2 cut(s)	17, 1016
GlaI	GCGC	6 cut(s)	182, 242, 449, 538, 654, 669
GluI	GCNGC	8 cut(s)	108, 173, 258, 345, 406, 460, 627, 741
GsaI	CCCAGC	1 cut(s)	554
HaeII	RGCGCY	3 cut(s)	451, 540, 656
HaeIII	GGCC	7 cut(s)	52, 278, 315, 323, 344, 405, 522
HapII	CCGG	1 cut(s)	244
HgaI	GACGC	1 cut(s)	512
HhaI	GCGC	6 cut(s)	183, 243, 450, 539, 655, 670
Hin1I	GRCGYC	1 cut(s)	448
Hin1II	CATG	8 cut(s)	232, 340, 445, 487, 831, 851, 902, 1013
Hin6I	GCGC	6 cut(s)	181, 241, 448, 537, 653, 668
HinP1I	GCGC	6 cut(s)	181, 241, 448, 537, 653, 668
HinfI	GANTC	6 cut(s)	116, 263, 510, 544, 748, 1157
HpaII	CCGG	1 cut(s)	244
HphI	GGTGA	4 cut(s)	67, 145, 610, 629
Hpy188I	TCNGA	7 cut(s)	96, 290, 543, 855, 916, 1117, 1187
Hpy188III	TCNNGA	6 cut(s)	46, 136, 190, 875, 1010, 1045
Hpy99I	CGWCG	3 cut(s)	181, 314, 329
HpyAV	CCTTC	7 cut(s)	64, 160, 406, 493, 784, 1021, 1042
HpyCH4III	ACNGT	7 cut(s)	167, 383, 527, 598, 617, 623, 1192
HpyCH4IV	ACGT	4 cut(s)	131, 215, 602, 940
HpyCH4V	TGCA	6 cut(s)	366, 393, 424, 487, 626, 831
HpyF10VI	GCNNNNNNNGC	4 cut(s)	178, 445, 456, 933
HpyF3I	CTNAG	4 cut(s)	47, 540, 1023, 1155
HpySE526I	ACGT	4 cut(s)	131, 215, 602, 940
Hsp92I	GRCGYC	1 cut(s)	448
Hsp92II	CATG	8 cut(s)	232, 340, 445, 487, 831, 851, 902, 1013
HspAI	GCGC	6 cut(s)	181, 241, 448, 537, 653, 668
KasI	GGCGCC	1 cut(s)	447
KflI	GGGWCCC	1 cut(s)	414
KpnI	GGTACC	2 cut(s)	726, 898
KspI	CCGCGG	1 cut(s)	409
Kzo9I	GATC	8 cut(s)	96, 252, 285, 676, 916, 973, 1012, 1030
LmnI	GCTCC	5 cut(s)	41, 205, 467, 844, 967
Lsp1109I	GCAGC	4 cut(s)	269, 446, 638, 752
LweI	GCATC	3 cut(s)	228, 375, 1092
MaeI	CTAG	2 cut(s)	17, 1016
MaeII	ACGT	4 cut(s)	131, 215, 602, 940
MaeIII	GTNAC	4 cut(s)	383, 598, 617, 902
MalI	GATC	8 cut(s)	98, 254, 287, 678, 918, 975, 1014, 1032
MboI	GATC	8 cut(s)	96, 252, 285, 676, 916, 973, 1012, 1030
MboII	GAAGA	4 cut(s)	211, 757, 868, 1114
MfeI	CAATTG	1 cut(s)	978
MflI	RGATCY	2 cut(s)	676, 1030
MhlI	GDGCHC	1 cut(s)	441
MlsI	TGGCCA	1 cut(s)	522
MluCI	AATT	2 cut(s)	833, 978
MluNI	TGGCCA	1 cut(s)	522
Mly113I	GGCGCC	1 cut(s)	448
MlyI	GAGTC	4 cut(s)	110, 257, 757, 1166
Mox20I	TGGCCA	1 cut(s)	522
Mph1103I	ATGCAT	1 cut(s)	489
MscI	TGGCCA	1 cut(s)	522
MseI	TTAA	1 cut(s)	657
MslI	CAYNNNNRTG	2 cut(s)	165, 335
Msp20I	TGGCCA	1 cut(s)	522
MspA1I	CMGCKG	2 cut(s)	408, 550
MspI	CCGG	1 cut(s)	244
MspR9I	CCNGG	4 cut(s)	317, 452, 783, 921
MunI	CAATTG	1 cut(s)	978
MvaI	CCWGG	4 cut(s)	317, 452, 783, 921
MvnI	CGCG	4 cut(s)	181, 225, 347, 408
MwoI	GCNNNNNNNGC	4 cut(s)	178, 445, 456, 933
NarI	GGCGCC	1 cut(s)	448
NcoI	CCATGG	1 cut(s)	898
NdeII	GATC	8 cut(s)	96, 252, 285, 676, 916, 973, 1012, 1030
NlaIII	CATG	8 cut(s)	232, 340, 445, 487, 831, 851, 902, 1013
NlaIV	GGNNCC	8 cut(s)	37, 415, 416, 449, 481, 724, 865, 896
NmeAIII	GCCGAG	3 cut(s)	381, 460, 754
NmuCI	GTSAC	4 cut(s)	383, 598, 617, 902
NsiI	ATGCAT	1 cut(s)	489
NspI	RCATGY	2 cut(s)	831, 851
OliI	CACNNNNGTG	1 cut(s)	165
PaeI	GCATGC	2 cut(s)	831, 851
PaeR7I	CTCGAG	1 cut(s)	188
PagI	TCATGA	1 cut(s)	1009
PfeI	GAWTC	2 cut(s)	510, 544
PkrI	GCNGC	8 cut(s)	109, 174, 259, 346, 407, 461, 628, 742
PleI	GAGTC	4 cut(s)	110, 257, 756, 1165
PluTI	GGCGCC	1 cut(s)	451
PmaCI	CACGTG	1 cut(s)	941
PmlI	CACGTG	1 cut(s)	941
PpsI	GAGTC	4 cut(s)	110, 257, 756, 1165
Ppu21I	YACGTR	1 cut(s)	941
PpuMI	RGGWCCY	1 cut(s)	414
Psp124BI	GAGCTC	1 cut(s)	441
Psp5II	RGGWCCY	1 cut(s)	414
Psp6I	CCWGG	4 cut(s)	315, 450, 781, 919
PspCI	CACGTG	1 cut(s)	941
PspFI	CCCAGC	1 cut(s)	550
PspGI	CCWGG	4 cut(s)	315, 450, 781, 919
PspN4I	GGNNCC	8 cut(s)	37, 415, 416, 449, 481, 724, 865, 896
PspPI	GGNCC	5 cut(s)	51, 414, 480, 863, 1166
PspPPI	RGGWCCY	1 cut(s)	414
PstI	CTGCAG	1 cut(s)	426
PsuI	RGATCY	2 cut(s)	676, 1030
RsaI	GTAC	5 cut(s)	380, 581, 724, 896, 1194
RsaNI	GTAC	5 cut(s)	379, 580, 723, 895, 1193
RseI	CAYNNNNRTG	2 cut(s)	165, 335
SacI	GAGCTC	1 cut(s)	441
SacII	CCGCGG	1 cut(s)	409
SaqAI	TTAA	1 cut(s)	657
SatI	GCNGC	8 cut(s)	108, 173, 258, 345, 406, 460, 627, 741
Sau3AI	GATC	8 cut(s)	96, 252, 285, 676, 916, 973, 1012, 1030
Sau96I	GGNCC	5 cut(s)	51, 414, 480, 863, 1166
SbfI	CCTGCAGG	1 cut(s)	426
ScaI	AGTACT	1 cut(s)	581
SchI	GAGTC	4 cut(s)	110, 257, 757, 1166
ScrFI	CCNGG	4 cut(s)	317, 452, 783, 921
SdaI	CCTGCAGG	1 cut(s)	426
SduI	GDGCHC	1 cut(s)	441
SfaNI	GCATC	3 cut(s)	228, 375, 1092
SfcI	CTRYAG	1 cut(s)	422
SfoI	GGCGCC	1 cut(s)	449
Sfr274I	CTCGAG	1 cut(s)	188
Sfr303I	CCGCGG	1 cut(s)	409
SgrBI	CCGCGG	1 cut(s)	409
SinI	GGWCC	4 cut(s)	414, 480, 863, 1166
SlaI	CTCGAG	1 cut(s)	188
SmiMI	CAYNNNNRTG	2 cut(s)	165, 335
SmlI	CTYRAG	2 cut(s)	188, 1083
SmoI	CTYRAG	2 cut(s)	188, 1083
SphI	GCATGC	2 cut(s)	831, 851
Sse8387I	CCTGCAGG	1 cut(s)	426
Sse9I	AATT	2 cut(s)	833, 978
SspDI	GGCGCC	1 cut(s)	447
SspMI	CTAG	2 cut(s)	17, 1016
SstI	GAGCTC	1 cut(s)	441
StyD4I	CCNGG	4 cut(s)	315, 450, 781, 919
StyI	CCWWGG	5 cut(s)	201, 465, 585, 840, 898
TaaI	ACNGT	7 cut(s)	167, 383, 527, 598, 617, 623, 1192
TaiI	ACGT	4 cut(s)	134, 218, 605, 943
TaqI	TCGA	4 cut(s)	135, 189, 648, 1044
TasI	AATT	2 cut(s)	833, 978
TatI	WGTACW	2 cut(s)	579, 1192
TauI	GCSGC	4 cut(s)	110, 175, 347, 408
TfiI	GAWTC	2 cut(s)	510, 544
Tru1I	TTAA	1 cut(s)	657
Tru9I	TTAA	1 cut(s)	657
TscAI	CASTG	2 cut(s)	379, 395
TseFI	GTSAC	4 cut(s)	383, 598, 617, 902
TseI	GCWGC	4 cut(s)	257, 459, 626, 740
Tsp45I	GTSAC	4 cut(s)	383, 598, 617, 902
TspDTI	ATGAA	1 cut(s)	1086
TspGWI	ACGGA	1 cut(s)	876
TspRI	CASTG	2 cut(s)	379, 395
VpaK11BI	GGWCC	4 cut(s)	414, 480, 863, 1166
XagI	CCTNNNNNAGG	1 cut(s)	422
XapI	RAATTY	1 cut(s)	833
XceI	RCATGY	2 cut(s)	831, 851
XhoI	CTCGAG	1 cut(s)	188
XspI	CTAG	2 cut(s)	17, 1016
ZrmI	AGTACT	1 cut(s)	581
Zsp2I	ATGCAT	1 cut(s)	489

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.