Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0006340

Physical Location & Seq

Genomic Coordinates Reverse (-)

6173559 .. 6176010

Gene Structure 2452 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1088100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1092 bp

ATGCCTTTCGGTTTAGTTTCGGCATGGAACAAGCGTCGGCGGTCGAAGTCCCACGACCTTACAGACCCTTGGATTTATAAACCGGTGGAGTACTGGCAGCTCGAGGATCAAACCCCCCAACCGACGAAAAGACACCATCATGGCTCGTCAATTTTCACACTCAAAGAAATGGAAGAGGCAACGTGTTCCTTCAGTGAAGAAAATCTGCTCGGAAAAGGAGGATTTGGGCGTGTATACCGGGGAACTTTGCGGTCAGGAGAGGTTGTAGCCATCAAGAAAATGGAGCTGCCTCCTTTCAAAGCAGCTGAGGGAGAACGAGAATTTCGGGTGGAAGTAGATATCTTGAGCAGACTTGAACACCCAAATCTGGTTTCTTTGATCGGCTATTGTGCTGACGGGAAGCAACGGTTTCTGGTGTATGAATATATGCAGAAAGGGAACCTGCAAGATCACTTAAATGGGATCGGGGAAGCGAAAATGGATTGGCCTCGACGGCTCAAGGTGGCACTTGGAGCAGCAAGGGGACTAGCGTATCTGCATTCTAGTTCTGCTGTCGGGATTCCTATTGTTCACAGAGATTTCAAATCCACCAATATTCTTTTAAGCGACGACTTTGAAGCAAAGATATCGGATTTTGGACTCGCCAAGATGATGCCGGAGGGCAAGGAAATATACGTGACTGCTAGAGTGCTCGGAACATTCGGCTATTTTGATCCCGAGTATACATCGACTGGAAAACTCACTTTACAAAGTGATGTTTATGCATTTGGAGTGGTTCTTCTTGAACTTCTGACTGGACGTCAAGCCGTTGACATTACCCTGGGACCAAACGATCAAAACCTAGTGCTACAGGTAAGACACATATTGAACGACAGGAAGAAACTGCGTAAGGTGATAGATTCTGAGCTGAGTCGAAGTTCATACACCATGGAATCTATAGCTATGTTTGCAAACCTGGCATCAAGATGTGTGCGTCCGGAGAGCAGTGAGAGACCCTCCATGGCAGAATGTGTGAAAGAGCTCCAGTTGAGTATCTATACAAATGCGAAAGGCTTGGGAGTGGCCGTGGCTCCTAACAAACACATGTTCTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002218 GO:0002221 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0002757 GO:0002758 GO:0002764 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009814 GO:0009816 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0031347 GO:0031349 GO:0036211 GO:0038023 GO:0042742 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0046777 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0080134 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase

Protein Analysis

364

Amino Acids

40.92

Weight (kDa)

8.69

Isoelectric Point (pI)

45.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	65 - 339	7.6e-47	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	67 - 341	9.3e-47	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006340)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G24030 AT1G24030 AT1G24030 AT1G24030 AT1G24030 AT1G24030
fragaria_vesca	FvH4_4g34900 FvH4_4g34900 FvH4_4g34900 FvH4_4g34900
malus_domestica	MD13G1088100.v1.1 MD16G1088500.v1.1
prunus_persica	Prupe.1G258200_v2.0.a1 Prupe.1G258200_v2.0.a1 Prupe.1G258200_v2.0.a1
pyrus_communis	pycom13g07590 pycom16g07570
rosa_chinensis	RchiOBHm_Chr4g0444341
rosa_laevigata	RLG00000005839
rosa_multiflora	Rmu_sc0002841.1_g000011
rosa_roxburghii	Rroxscaffold_5G00384880
rosa_rugosa	Rorug04G0351600 Rorug04G0351700
rosa_samantha	Rh4AG412400 Rh4BG423900 Rh4DG419100
rosa_wichuraiana	Rw4G035420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	78
AatII	GACGTC	1 cut(s)	802
Acc36I	ACCTGC	1 cut(s)	450
AccI	GTMKAC	2 cut(s)	234, 722
AccIII	TCCGGA	1 cut(s)	976
AciI	CCGC	2 cut(s)	40, 250
AclWI	GGATC	3 cut(s)	114, 470, 707
AcoI	YGGCCR	1 cut(s)	1062
AcsI	RAATTY	1 cut(s)	320
AcuI	CTGAAG	1 cut(s)	175
AcyI	GRCGYC	1 cut(s)	799
AfaI	GTAC	1 cut(s)	92
AfiI	CCNNNNNNNGG	1 cut(s)	367
AflIII	ACRYGT	2 cut(s)	182, 1083
AgeI	ACCGGT	1 cut(s)	82
AgsI	TTSAA	6 cut(s)	298, 356, 583, 617, 785, 868
AhdI	GACNNNNNGTC	1 cut(s)	798
AjnI	CCWGG	2 cut(s)	819, 954
AjuI	GAANNNNNNNTTGG	2 cut(s)	355, 387
AluBI	AGCT	6 cut(s)	100, 286, 305, 907, 941, 1021
AluI	AGCT	6 cut(s)	100, 286, 305, 907, 941, 1021
Alw21I	GWGCWC	2 cut(s)	693, 1023
Alw26I	GTCTC	1 cut(s)	985
AlwI	GGATC	3 cut(s)	114, 470, 707
Ama87I	CYCGRG	2 cut(s)	101, 716
Aor13HI	TCCGGA	1 cut(s)	976
AoxI	GGCC	2 cut(s)	485, 1062
ApeKI	GCWGC	4 cut(s)	97, 286, 302, 515
ApoI	RAATTY	1 cut(s)	320
AsiGI	ACCGGT	1 cut(s)	82
AspS9I	GGNCC	1 cut(s)	824
AsuC2I	CCSGG	1 cut(s)	239
AsuHPI	GGTGA	1 cut(s)	904
AvaI	CYCGRG	2 cut(s)	101, 716
AvaII	GGWCC	1 cut(s)	824
BaeI	ACNNNNGTAYC	2 cut(s)	515, 548
BanII	GRGCYC	1 cut(s)	1023
Bbv12I	GWGCWC	2 cut(s)	693, 1023
BbvCI	CCTCAGC	1 cut(s)	306
BbvI	GCAGC	4 cut(s)	109, 273, 314, 527
BccI	CCATC	2 cut(s)	144, 278
BceAI	ACGGC	3 cut(s)	509, 791, 1049
BcgI	CGANNNNNNTGC	2 cut(s)	609, 643
BciT130I	CCWGG	2 cut(s)	821, 956
BcnI	CCSGG	1 cut(s)	239
BcoDI	GTCTC	1 cut(s)	985
BfaI	CTAG	4 cut(s)	527, 543, 684, 842
BfmI	CTRYAG	2 cut(s)	848, 936
BfuAI	ACCTGC	1 cut(s)	450
BglI	GCCNNNNNGGC	1 cut(s)	493
BisI	GCNGC	4 cut(s)	98, 287, 303, 516
BlsI	GCNGC	4 cut(s)	99, 288, 304, 517
BmcAI	AGTACT	1 cut(s)	92
Bme1390I	CCNGG	3 cut(s)	239, 821, 956
Bme18I	GGWCC	1 cut(s)	824
BmeRI	GACNNNNNGTC	1 cut(s)	798
BmeT110I	CYCGRG	2 cut(s)	101, 716
BmgT120I	GGNCC	1 cut(s)	824
BmiI	GGNNCC	3 cut(s)	440, 825, 1071
BmrFI	CCNGG	3 cut(s)	239, 821, 956
BmsI	GCATC	2 cut(s)	642, 968
BplI	GAGNNNNNCTC	2 cut(s)	980, 1012
BpmI	CTGGAG	1 cut(s)	1007
Bpu10I	CCTNAGC	1 cut(s)	306
BpuEI	CTTGAG	2 cut(s)	364, 482
BpuMI	CCSGG	1 cut(s)	239
BsaAI	YACGTR	1 cut(s)	676
BsaHI	GRCGYC	1 cut(s)	799
BsaI	GGTCTC	1 cut(s)	985
BsaJI	CCNNGG	7 cut(s)	68, 238, 819, 820, 927, 999, 1065
BsaWI	WCCGGW	2 cut(s)	82, 976
BsaXI	ACNNNNNCTCC	4 cut(s)	249, 279, 1050, 1080
Bsc4I	CCNNNNNNNGG	1 cut(s)	367
Bse118I	RCCGGY	1 cut(s)	82
Bse1I	ACTGG	4 cut(s)	98, 736, 799, 1024
BseAI	TCCGGA	1 cut(s)	976
BseBI	CCWGG	2 cut(s)	821, 956
BseDI	CCNNGG	7 cut(s)	68, 238, 819, 820, 927, 999, 1065
BseLI	CCNNNNNNNGG	1 cut(s)	367
BseMII	CTCAG	3 cut(s)	297, 894, 899
BseNI	ACTGG	4 cut(s)	98, 736, 799, 1024
BseXI	GCAGC	4 cut(s)	109, 273, 314, 527
Bsh1285I	CGRYCG	1 cut(s)	44
BshFI	GGCC	2 cut(s)	487, 1064
BshTI	ACCGGT	1 cut(s)	82
BsiEI	CGRYCG	1 cut(s)	44
BsiHKAI	GWGCWC	2 cut(s)	693, 1023
BsiHKCI	CYCGRG	2 cut(s)	101, 716
BsiSI	CCGG	4 cut(s)	83, 238, 656, 977
BslFI	GGGAC	3 cut(s)	34, 537, 837
BslI	CCNNNNNNNGG	1 cut(s)	367
BsmAI	GTCTC	1 cut(s)	985
BsmFI	GGGAC	3 cut(s)	34, 537, 837
BsmI	GAATGC	1 cut(s)	538
BsnI	GGCC	2 cut(s)	487, 1064
Bso31I	GGTCTC	1 cut(s)	985
BsoBI	CYCGRG	2 cut(s)	101, 716
Bsp1286I	GDGCHC	2 cut(s)	693, 1023
Bsp13I	TCCGGA	1 cut(s)	976
Bsp143I	GATC	6 cut(s)	106, 378, 448, 462, 712, 832
Bsp19I	CCATGG	2 cut(s)	927, 999
BspACI	CCGC	2 cut(s)	40, 250
BspANI	GGCC	2 cut(s)	487, 1064
BspCNI	CTCAG	3 cut(s)	298, 895, 900
BspEI	TCCGGA	1 cut(s)	976
BspLI	GGNNCC	3 cut(s)	440, 825, 1071
BspMI	ACCTGC	1 cut(s)	450
BspPI	GGATC	3 cut(s)	114, 470, 707
BspTNI	GGTCTC	1 cut(s)	985
BsrFI	RCCGGY	1 cut(s)	82
BsrI	ACTGG	4 cut(s)	98, 736, 799, 1024
BssAI	RCCGGY	1 cut(s)	82
BssECI	CCNNGG	7 cut(s)	68, 238, 819, 820, 927, 999, 1065
BssMI	GATC	6 cut(s)	106, 378, 448, 462, 712, 832
BssNAI	GTATAC	2 cut(s)	235, 723
BssNI	GRCGYC	1 cut(s)	799
BssT1I	CCWWGG	3 cut(s)	68, 927, 999
Bst1107I	GTATAC	2 cut(s)	235, 723
Bst2UI	CCWGG	2 cut(s)	821, 956
Bst4CI	ACNGT	1 cut(s)	408
Bst6I	CTCTTC	1 cut(s)	168
BstACI	GRCGYC	1 cut(s)	799
BstBAI	YACGTR	1 cut(s)	676
BstDEI	CTNAG	3 cut(s)	306, 903, 908
BstDSI	CCRYGG	3 cut(s)	927, 999, 1065
BstKTI	GATC	6 cut(s)	109, 381, 451, 465, 715, 835
BstMAI	GTCTC	1 cut(s)	985
BstMBI	GATC	6 cut(s)	106, 378, 448, 462, 712, 832
BstMCI	CGRYCG	1 cut(s)	44
BstMWI	GCNNNNNNNGC	4 cut(s)	493, 512, 947, 956
BstNI	CCWGG	2 cut(s)	821, 956
BstNSI	RCATGY	1 cut(s)	1087
BstSCI	CCNGG	3 cut(s)	237, 819, 954
BstSFI	CTRYAG	2 cut(s)	848, 936
BstV1I	GCAGC	4 cut(s)	109, 273, 314, 527
BstZ17I	GTATAC	2 cut(s)	235, 723
BsuRI	GGCC	2 cut(s)	487, 1064
BtgI	CCRYGG	3 cut(s)	927, 999, 1065
BtsI	GCAGTG	1 cut(s)	991
BtsIMutI	CAGTG	2 cut(s)	199, 991
BveI	ACCTGC	1 cut(s)	450
Cfr10I	RCCGGY	1 cut(s)	82
Cfr13I	GGNCC	1 cut(s)	824
CseI	GACGC	2 cut(s)	23, 962
Csp6I	GTAC	1 cut(s)	91
CspAI	ACCGGT	1 cut(s)	82
CviAII	CATG	5 cut(s)	24, 140, 928, 1000, 1084
CviQI	GTAC	1 cut(s)	91
DdeI	CTNAG	3 cut(s)	306, 903, 908
DpnI	GATC	6 cut(s)	108, 380, 450, 464, 714, 834
DpnII	GATC	6 cut(s)	106, 378, 448, 462, 712, 832
DriI	GACNNNNNGTC	1 cut(s)	798
EaeI	YGGCCR	1 cut(s)	1062
Eam1104I	CTCTTC	1 cut(s)	168
Eam1105I	GACNNNNNGTC	1 cut(s)	798
EarI	CTCTTC	1 cut(s)	168
Ecl136II	GAGCTC	1 cut(s)	1021
Eco130I	CCWWGG	3 cut(s)	68, 927, 999
Eco24I	GRGCYC	1 cut(s)	1023
Eco31I	GGTCTC	1 cut(s)	985
Eco32I	GATATC	2 cut(s)	340, 627
Eco47I	GGWCC	1 cut(s)	824
Eco53kI	GAGCTC	1 cut(s)	1021
Eco57I	CTGAAG	1 cut(s)	175
Eco88I	CYCGRG	2 cut(s)	101, 716
EcoICRI	GAGCTC	1 cut(s)	1021
EcoRII	CCWGG	2 cut(s)	819, 954
EcoRV	GATATC	2 cut(s)	340, 627
EcoT14I	CCWWGG	3 cut(s)	68, 927, 999
EcoT22I	ATGCAT	1 cut(s)	766
EcoT38I	GRGCYC	1 cut(s)	1023
ErhI	CCWWGG	3 cut(s)	68, 927, 999
FaeI	CATG	5 cut(s)	27, 143, 931, 1003, 1087
FaqI	GGGAC	3 cut(s)	34, 537, 837
FatI	CATG	5 cut(s)	23, 139, 927, 999, 1083
FblI	GTMKAC	2 cut(s)	234, 722
Fnu4HI	GCNGC	4 cut(s)	98, 287, 303, 516
FriOI	GRGCYC	1 cut(s)	1023
Fsp4HI	GCNGC	4 cut(s)	98, 287, 303, 516
FspBI	CTAG	4 cut(s)	527, 543, 684, 842
GluI	GCNGC	4 cut(s)	98, 287, 303, 516
GsuI	CTGGAG	1 cut(s)	1007
HaeIII	GGCC	2 cut(s)	487, 1064
HapII	CCGG	4 cut(s)	83, 238, 656, 977
HgaI	GACGC	2 cut(s)	23, 962
Hin1I	GRCGYC	1 cut(s)	799
Hin1II	CATG	5 cut(s)	27, 143, 931, 1003, 1087
HincII	GTYRAC	1 cut(s)	811
HindII	GTYRAC	1 cut(s)	811
HinfI	GANTC	5 cut(s)	559, 639, 899, 910, 932
HpaII	CCGG	4 cut(s)	83, 238, 656, 977
HphI	GGTGA	1 cut(s)	904
Hpy166II	GTNNAC	4 cut(s)	235, 571, 723, 811
Hpy188I	TCNGA	5 cut(s)	212, 631, 695, 792, 904
Hpy188III	TCNNGA	8 cut(s)	255, 274, 343, 556, 716, 782, 963, 977
Hpy8I	GTNNAC	4 cut(s)	235, 571, 723, 811
Hpy99I	CGWCG	4 cut(s)	39, 127, 495, 611
HpyAV	CCTTC	1 cut(s)	199
HpyCH4III	ACNGT	1 cut(s)	408
HpyCH4IV	ACGT	3 cut(s)	182, 675, 799
HpyCH4V	TGCA	5 cut(s)	430, 445, 538, 764, 950
HpyF10VI	GCNNNNNNNGC	4 cut(s)	493, 512, 947, 956
HpyF3I	CTNAG	3 cut(s)	306, 903, 908
HpySE526I	ACGT	3 cut(s)	182, 675, 799
Hsp92I	GRCGYC	1 cut(s)	799
Hsp92II	CATG	5 cut(s)	27, 143, 931, 1003, 1087
Kpn2I	TCCGGA	1 cut(s)	976
Kzo9I	GATC	6 cut(s)	106, 378, 448, 462, 712, 832
LmnI	GCTCC	4 cut(s)	283, 512, 1026, 1075
Lsp1109I	GCAGC	4 cut(s)	109, 273, 314, 527
LweI	GCATC	2 cut(s)	642, 968
MaeI	CTAG	4 cut(s)	527, 543, 684, 842
MaeII	ACGT	3 cut(s)	182, 675, 799
MaeIII	GTNAC	1 cut(s)	676
MalI	GATC	6 cut(s)	108, 380, 450, 464, 714, 834
MboI	GATC	6 cut(s)	106, 378, 448, 462, 712, 832
MboII	GAAGA	4 cut(s)	185, 209, 770, 889
MhlI	GDGCHC	2 cut(s)	693, 1023
MluCI	AATT	2 cut(s)	150, 320
MlyI	GAGTC	2 cut(s)	633, 919
MnlI	CCTC	9 cut(s)	97, 169, 212, 253, 300, 301, 498, 652, 1006
Mph1103I	ATGCAT	1 cut(s)	766
MroI	TCCGGA	1 cut(s)	976
MseI	TTAA	2 cut(s)	455, 602
MslI	CAYNNNNRTG	3 cut(s)	138, 456, 964
MspA1I	CMGCKG	1 cut(s)	305
MspI	CCGG	4 cut(s)	83, 238, 656, 977
MspR9I	CCNGG	3 cut(s)	239, 821, 956
Mva1269I	GAATGC	1 cut(s)	538
MvaI	CCWGG	2 cut(s)	821, 956
MwoI	GCNNNNNNNGC	4 cut(s)	493, 512, 947, 956
NciI	CCSGG	1 cut(s)	239
NcoI	CCATGG	2 cut(s)	927, 999
NdeII	GATC	6 cut(s)	106, 378, 448, 462, 712, 832
NlaIII	CATG	5 cut(s)	27, 143, 931, 1003, 1087
NlaIV	GGNNCC	3 cut(s)	440, 825, 1071
NmuCI	GTSAC	1 cut(s)	676
NsiI	ATGCAT	1 cut(s)	766
NspI	RCATGY	1 cut(s)	1087
PaeR7I	CTCGAG	1 cut(s)	101
PasI	CCCWGGG	1 cut(s)	820
PciI	ACATGT	1 cut(s)	1083
PctI	GAATGC	1 cut(s)	538
PfeI	GAWTC	3 cut(s)	559, 899, 932
PinAI	ACCGGT	1 cut(s)	82
PkrI	GCNGC	4 cut(s)	99, 288, 304, 517
PleI	GAGTC	2 cut(s)	633, 918
PpsI	GAGTC	2 cut(s)	633, 918
Ppu21I	YACGTR	1 cut(s)	676
PscI	ACATGT	1 cut(s)	1083
PsiI	TTATAA	1 cut(s)	78
Psp124BI	GAGCTC	1 cut(s)	1023
Psp6I	CCWGG	2 cut(s)	819, 954
PspGI	CCWGG	2 cut(s)	819, 954
PspN4I	GGNNCC	3 cut(s)	440, 825, 1071
PspPI	GGNCC	1 cut(s)	824
PspXI	VCTCGAGB	1 cut(s)	101
PvuII	CAGCTG	1 cut(s)	305
RsaI	GTAC	1 cut(s)	92
RsaNI	GTAC	1 cut(s)	91
RseI	CAYNNNNRTG	3 cut(s)	138, 456, 964
SacI	GAGCTC	1 cut(s)	1023
SaqAI	TTAA	2 cut(s)	455, 602
SatI	GCNGC	4 cut(s)	98, 287, 303, 516
Sau3AI	GATC	6 cut(s)	106, 378, 448, 462, 712, 832
Sau96I	GGNCC	1 cut(s)	824
ScaI	AGTACT	1 cut(s)	92
SchI	GAGTC	2 cut(s)	633, 919
ScrFI	CCNGG	3 cut(s)	239, 821, 956
SduI	GDGCHC	2 cut(s)	693, 1023
SfaNI	GCATC	2 cut(s)	642, 968
SfcI	CTRYAG	2 cut(s)	848, 936
Sfr274I	CTCGAG	1 cut(s)	101
SinI	GGWCC	1 cut(s)	824
SlaI	CTCGAG	1 cut(s)	101
SmiMI	CAYNNNNRTG	3 cut(s)	138, 456, 964
SmlI	CTYRAG	3 cut(s)	101, 343, 497
SmoI	CTYRAG	3 cut(s)	101, 343, 497
Sse9I	AATT	2 cut(s)	150, 320
SsiI	CCGC	2 cut(s)	40, 250
SspI	AATATT	1 cut(s)	595
SspMI	CTAG	4 cut(s)	527, 543, 684, 842
SstI	GAGCTC	1 cut(s)	1023
StyD4I	CCNGG	3 cut(s)	237, 819, 954
StyI	CCWWGG	3 cut(s)	68, 927, 999
TaaI	ACNGT	1 cut(s)	408
TaiI	ACGT	3 cut(s)	185, 678, 802
TaqI	TCGA	5 cut(s)	44, 102, 490, 728, 913
TasI	AATT	2 cut(s)	150, 320
TatI	WGTACW	1 cut(s)	90
TfiI	GAWTC	3 cut(s)	559, 899, 932
Tru1I	TTAA	2 cut(s)	455, 602
Tru9I	TTAA	2 cut(s)	455, 602
TscAI	CASTG	2 cut(s)	199, 991
TseFI	GTSAC	1 cut(s)	676
TseI	GCWGC	4 cut(s)	97, 286, 302, 515
Tsp45I	GTSAC	1 cut(s)	676
TspDTI	ATGAA	2 cut(s)	435, 909
TspRI	CASTG	2 cut(s)	199, 991
VpaK11BI	GGWCC	1 cut(s)	824
XapI	RAATTY	1 cut(s)	320
XceI	RCATGY	1 cut(s)	1087
XcmI	CCANNNNNNNNNTGG	1 cut(s)	277
XhoI	CTCGAG	1 cut(s)	101
XmiI	GTMKAC	2 cut(s)	234, 722
XspI	CTAG	4 cut(s)	527, 543, 684, 842
ZraI	GACGTC	1 cut(s)	800
ZrmI	AGTACT	1 cut(s)	92
Zsp2I	ATGCAT	1 cut(s)	766

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.