Basic Information

Type: gene

Biological Identity

Species

rosa_wichuraiana

Chromosome

Chr4

Orthogroup ID

OG0006340

Physical Location & Seq

Genomic Coordinates Forward (+)

62223006 .. 62224897

Gene Structure 1892 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rw4G035420.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1086 bp

ATGCCTTTTGGTTTAGTCTCAGCATGGAACAAGCGCAGAAGGTCCAAATCCTATGACCACACAGACCCTTGGACTTATAAGCCAGTGGAGTTCTGGCAACTAAATGATCAAACACCTCAACAAACGAAAAGGCACCATCATGGCTCTTCAGTGTTTACACTCAAAGAAATGGACGAGGCAACATCTTCATTCAGTGACAAGAACTTTCTTGGGAAAGGAGGATTTGGCCGCGTATATAGAGGAACTTTGCGCTCAGGAGAGGTTGTAGCAATTAAAAAGATGGAGCTGCCTCCCTTTAAAGCAGCTGACGGAGAACGAGAATTTCGAGTAGAAGTAGACATCTTGAGCAGACTTGATCACCCAAATTTGGTTTCTTTGATAGGCTATTGTGCTGATGGCAAGCACCGGTTTTTAGTATATGAATACCTGCAGAAAGGGAACCTGCAAGATCACTTAAATGGAATTAATGAGAAACACAAAATGGGTTGGCCTCAAAGGCTCAGAGTGGCACTTGGAGCAGCAAGGGGCTTAGCATATCTACATTCGAGTTCAACTCCTATTGTTCACAGAGATTTCAAATCCACCAACATTCTTTTAGATGCCAACTTGAAGCAAAAGGATATAGCTGAAATGTTGATAACTGTTCTGCAGATATCCGATTTTGGGCTTGCCAAGTTGATGCCAGAGGGAAAGGAAATGTATGTGACTGCTAGAGTACTTGGCACATTCGGCTATTTTGATCCGGAGTATACATCGACAGGAAAACTCACTTTACAGAGTGATGTTTATGCATTTGGAGTGGTTCTTCTTGAACTTCTGACTGGACGTCAAGCCCTGGACCTAAACCAAGGACCAAATGATCAAAATCTTGTACTACAGGTAAAGCACATATTAAATGATCGGAAGAAGCTGCGTAAGGTCATAGATCCTGAGCTGGGTCGAAGTTCATACACCATGGAATCTATAGTTATGTTTGCAAACCTGGCATCACGATGTGTGCGTCCTGACAGCAGTGAAAGACCCTCCATGGCTGAATGTGTAAAAGAACTGCAGTTGATTATCATTACAAATGCAAAAGGCTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002218 GO:0002221 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0002757 GO:0002758 GO:0002764 GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004675 GO:0004888 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007154 GO:0007165 GO:0007166 GO:0007167 GO:0007178 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009617 GO:0009814 GO:0009816 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019199 GO:0019538 GO:0023052 GO:0031347 GO:0031349 GO:0036211 GO:0038023 GO:0042742 GO:0043170 GO:0043207 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0046777 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0060089 GO:0065007 GO:0071704 GO:0071944 GO:0080134 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

Pkinase

Protein Analysis

361

Amino Acids

40.92

Weight (kDa)

9.19

Isoelectric Point (pI)

41.59

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	65 - 348	3.4e-46	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	68 - 350	1.2e-43	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006340)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G24030 AT1G24030 AT1G24030 AT1G24030 AT1G24030 AT1G24030
fragaria_vesca	FvH4_4g34900 FvH4_4g34900 FvH4_4g34900 FvH4_4g34900
malus_domestica	MD13G1088100.v1.1 MD16G1088500.v1.1
prunus_persica	Prupe.1G258200_v2.0.a1 Prupe.1G258200_v2.0.a1 Prupe.1G258200_v2.0.a1
pyrus_communis	pycom13g07590 pycom16g07570
rosa_chinensis	RchiOBHm_Chr4g0444341
rosa_laevigata	RLG00000005839
rosa_multiflora	Rmu_sc0002841.1_g000011
rosa_roxburghii	Rroxscaffold_5G00384880
rosa_rugosa	Rorug04G0351600 Rorug04G0351700
rosa_samantha	Rh4AG412400 Rh4BG423900 Rh4DG419100
rosa_wichuraiana	Rw4G035420

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	78
AatII	GACGTC	1 cut(s)	829
Acc36I	ACCTGC	2 cut(s)	435, 450
AccB1I	GGYRCC	1 cut(s)	132
AccI	GTMKAC	2 cut(s)	336, 749
AccII	CGCG	1 cut(s)	231
AccIII	TCCGGA	1 cut(s)	742
AciI	CCGC	1 cut(s)	229
AclWI	GGATC	2 cut(s)	734, 920
AcoI	YGGCCR	1 cut(s)	226
AcsI	RAATTY	2 cut(s)	320, 364
AcuI	CTGAAG	1 cut(s)	132
AcyI	GRCGYC	1 cut(s)	826
AdeI	CACNNNGTG	1 cut(s)	995
AfaI	GTAC	2 cut(s)	717, 873
AfiI	CCNNNNNNNGG	3 cut(s)	367, 663, 935
AgeI	ACCGGT	1 cut(s)	405
AgsI	TTSAA	4 cut(s)	552, 577, 610, 812
AhdI	GACNNNNNGTC	1 cut(s)	825
AjnI	CCWGG	2 cut(s)	834, 981
AjuI	GAANNNNNNNTTGG	2 cut(s)	355, 387
AluBI	AGCT	5 cut(s)	286, 305, 626, 910, 934
AluI	AGCT	5 cut(s)	286, 305, 626, 910, 934
Alw26I	GTCTC	1 cut(s)	22
AlwI	GGATC	2 cut(s)	734, 920
Aor13HI	TCCGGA	1 cut(s)	742
AoxI	GGCC	2 cut(s)	226, 488
ApeKI	GCWGC	4 cut(s)	286, 302, 518, 910
ApoI	RAATTY	2 cut(s)	320, 364
AseI	ATTAAT	1 cut(s)	465
AsiGI	ACCGGT	1 cut(s)	405
AspLEI	GCGC	2 cut(s)	36, 252
AspS9I	GGNCC	3 cut(s)	42, 838, 851
AsuHPI	GGTGA	1 cut(s)	350
AvaII	GGWCC	3 cut(s)	42, 838, 851
BanI	GGYRCC	1 cut(s)	132
BbvI	GCAGC	4 cut(s)	273, 314, 530, 897
BccI	CCATC	3 cut(s)	144, 274, 389
BciT130I	CCWGG	2 cut(s)	836, 983
BclI	TGATCA	3 cut(s)	106, 355, 859
BcoDI	GTCTC	1 cut(s)	22
BfaI	CTAG	1 cut(s)	711
BfmI	CTRYAG	5 cut(s)	428, 647, 875, 963, 1049
BfuAI	ACCTGC	2 cut(s)	435, 450
BglI	GCCNNNNNGGC	1 cut(s)	496
BisI	GCNGC	5 cut(s)	229, 287, 303, 519, 911
BlpI	GCTNAGC	1 cut(s)	529
BlsI	GCNGC	5 cut(s)	230, 288, 304, 520, 912
BmcAI	AGTACT	1 cut(s)	717
Bme1390I	CCNGG	2 cut(s)	836, 983
Bme18I	GGWCC	3 cut(s)	42, 838, 851
BmeRI	GACNNNNNGTC	1 cut(s)	825
BmgT120I	GGNCC	3 cut(s)	42, 838, 851
BmiI	GGNNCC	2 cut(s)	134, 440
BmrFI	CCNGG	2 cut(s)	836, 983
BmsI	GCATC	3 cut(s)	589, 669, 995
BplI	GAGNNNNNCTC	2 cut(s)	538, 570
Bpu10I	CCTNAGC	2 cut(s)	253, 930
Bpu1102I	GCTNAGC	1 cut(s)	529
BpuEI	CTTGAG	1 cut(s)	364
BsaBI	GATNNNNATC	1 cut(s)	864
BsaHI	GRCGYC	1 cut(s)	826
BsaJI	CCNNGG	5 cut(s)	68, 834, 847, 954, 1026
BsaWI	WCCGGW	2 cut(s)	405, 742
BsaXI	ACNNNNNCTCC	2 cut(s)	249, 279
Bsc4I	CCNNNNNNNGG	3 cut(s)	367, 663, 935
Bse118I	RCCGGY	1 cut(s)	405
Bse1I	ACTGG	2 cut(s)	83, 826
Bse8I	GATNNNNATC	1 cut(s)	864
BseAI	TCCGGA	1 cut(s)	742
BseBI	CCWGG	2 cut(s)	836, 983
BseDI	CCNNGG	5 cut(s)	68, 834, 847, 954, 1026
BseJI	GATNNNNATC	1 cut(s)	864
BseLI	CCNNNNNNNGG	3 cut(s)	367, 663, 935
BseMII	CTCAG	4 cut(s)	33, 267, 514, 921
BseNI	ACTGG	2 cut(s)	83, 826
BseXI	GCAGC	4 cut(s)	273, 314, 530, 897
BseYI	CCCAGC	1 cut(s)	934
Bsh1236I	CGCG	1 cut(s)	231
BshFI	GGCC	2 cut(s)	228, 490
BshNI	GGYRCC	1 cut(s)	132
BshTI	ACCGGT	1 cut(s)	405
BsiSI	CCGG	2 cut(s)	406, 743
BslI	CCNNNNNNNGG	3 cut(s)	367, 663, 935
BsmAI	GTCTC	1 cut(s)	22
BsnI	GGCC	2 cut(s)	228, 490
Bsp13I	TCCGGA	1 cut(s)	742
Bsp143I	GATC	7 cut(s)	106, 355, 448, 739, 859, 898, 925
Bsp1720I	GCTNAGC	1 cut(s)	529
Bsp19I	CCATGG	2 cut(s)	954, 1026
BspACI	CCGC	1 cut(s)	229
BspANI	GGCC	2 cut(s)	228, 490
BspCNI	CTCAG	4 cut(s)	32, 266, 513, 922
BspEI	TCCGGA	1 cut(s)	742
BspFNI	CGCG	1 cut(s)	231
BspLI	GGNNCC	2 cut(s)	134, 440
BspMAI	CTGCAG	3 cut(s)	432, 651, 1053
BspMI	ACCTGC	2 cut(s)	435, 450
BspPI	GGATC	2 cut(s)	734, 920
BspQI	GCTCTTC	1 cut(s)	151
BspT107I	GGYRCC	1 cut(s)	132
BsrFI	RCCGGY	1 cut(s)	405
BsrI	ACTGG	2 cut(s)	83, 826
BssAI	RCCGGY	1 cut(s)	405
BssECI	CCNNGG	5 cut(s)	68, 834, 847, 954, 1026
BssMI	GATC	7 cut(s)	106, 355, 448, 739, 859, 898, 925
BssNAI	GTATAC	1 cut(s)	750
BssNI	GRCGYC	1 cut(s)	826
BssT1I	CCWWGG	4 cut(s)	68, 847, 954, 1026
Bst1107I	GTATAC	1 cut(s)	750
Bst2UI	CCWGG	2 cut(s)	836, 983
Bst4CI	ACNGT	1 cut(s)	643
Bst6I	CTCTTC	1 cut(s)	151
BstACI	GRCGYC	1 cut(s)	826
BstC8I	GCNNGC	2 cut(s)	401, 669
BstDEI	CTNAG	5 cut(s)	19, 253, 500, 529, 930
BstDSI	CCRYGG	2 cut(s)	954, 1026
BstFNI	CGCG	1 cut(s)	231
BstHHI	GCGC	2 cut(s)	36, 252
BstKTI	GATC	7 cut(s)	109, 358, 451, 742, 862, 901, 928
BstMAI	GTCTC	1 cut(s)	22
BstMBI	GATC	7 cut(s)	106, 355, 448, 739, 859, 898, 925
BstMWI	GCNNNNNNNGC	4 cut(s)	496, 515, 729, 983
BstNI	CCWGG	2 cut(s)	836, 983
BstSCI	CCNGG	2 cut(s)	834, 981
BstSFI	CTRYAG	5 cut(s)	428, 647, 875, 963, 1049
BstUI	CGCG	1 cut(s)	231
BstV1I	GCAGC	4 cut(s)	273, 314, 530, 897
BstX2I	RGATCY	1 cut(s)	925
BstYI	RGATCY	1 cut(s)	925
BstZ17I	GTATAC	1 cut(s)	750
BsuRI	GGCC	2 cut(s)	228, 490
BtgI	CCRYGG	2 cut(s)	954, 1026
BtsI	GCAGTG	1 cut(s)	1018
BtsIMutI	CAGTG	4 cut(s)	90, 156, 199, 1018
BveI	ACCTGC	2 cut(s)	435, 450
Cac8I	GCNNGC	2 cut(s)	401, 669
CfoI	GCGC	2 cut(s)	36, 252
Cfr10I	RCCGGY	1 cut(s)	405
Cfr13I	GGNCC	3 cut(s)	42, 838, 851
CseI	GACGC	1 cut(s)	989
Csp6I	GTAC	2 cut(s)	716, 872
CspAI	ACCGGT	1 cut(s)	405
CviAII	CATG	4 cut(s)	24, 140, 955, 1027
CviQI	GTAC	2 cut(s)	716, 872
DdeI	CTNAG	5 cut(s)	19, 253, 500, 529, 930
DpnI	GATC	7 cut(s)	108, 357, 450, 741, 861, 900, 927
DpnII	GATC	7 cut(s)	106, 355, 448, 739, 859, 898, 925
DraI	TTTAAA	1 cut(s)	298
DraIII	CACNNNGTG	1 cut(s)	995
DriI	GACNNNNNGTC	1 cut(s)	825
EaeI	YGGCCR	1 cut(s)	226
Eam1104I	CTCTTC	1 cut(s)	151
Eam1105I	GACNNNNNGTC	1 cut(s)	825
EarI	CTCTTC	1 cut(s)	151
Eco130I	CCWWGG	4 cut(s)	68, 847, 954, 1026
Eco32I	GATATC	1 cut(s)	654
Eco47I	GGWCC	3 cut(s)	42, 838, 851
Eco57I	CTGAAG	1 cut(s)	132
EcoRII	CCWGG	2 cut(s)	834, 981
EcoRV	GATATC	1 cut(s)	654
EcoT14I	CCWWGG	4 cut(s)	68, 847, 954, 1026
EcoT22I	ATGCAT	1 cut(s)	793
ErhI	CCWWGG	4 cut(s)	68, 847, 954, 1026
FaeI	CATG	4 cut(s)	27, 143, 958, 1030
FatI	CATG	4 cut(s)	23, 139, 954, 1026
FbaI	TGATCA	3 cut(s)	106, 355, 859
FblI	GTMKAC	2 cut(s)	336, 749
Fnu4HI	GCNGC	5 cut(s)	229, 287, 303, 519, 911
Fsp4HI	GCNGC	5 cut(s)	229, 287, 303, 519, 911
FspBI	CTAG	1 cut(s)	711
GlaI	GCGC	2 cut(s)	35, 251
GluI	GCNGC	5 cut(s)	229, 287, 303, 519, 911
GsaI	CCCAGC	1 cut(s)	938
HaeIII	GGCC	2 cut(s)	228, 490
HapII	CCGG	2 cut(s)	406, 743
HgaI	GACGC	1 cut(s)	989
HhaI	GCGC	2 cut(s)	36, 252
Hin1I	GRCGYC	1 cut(s)	826
Hin1II	CATG	4 cut(s)	27, 143, 958, 1030
Hin6I	GCGC	2 cut(s)	34, 250
HinP1I	GCGC	2 cut(s)	34, 250
HinfI	GANTC	1 cut(s)	959
HpaII	CCGG	2 cut(s)	406, 743
HphI	GGTGA	1 cut(s)	350
Hpy166II	GTNNAC	4 cut(s)	156, 337, 565, 750
Hpy188I	TCNGA	4 cut(s)	503, 658, 819, 903
Hpy188III	TCNNGA	7 cut(s)	255, 343, 743, 809, 929, 990, 1004
Hpy8I	GTNNAC	4 cut(s)	156, 337, 565, 750
HpyAV	CCTTC	1 cut(s)	33
HpyCH4III	ACNGT	1 cut(s)	643
HpyCH4IV	ACGT	1 cut(s)	826
HpyCH4V	TGCA	7 cut(s)	430, 445, 649, 791, 977, 1051, 1073
HpyF10VI	GCNNNNNNNGC	4 cut(s)	496, 515, 729, 983
HpyF3I	CTNAG	5 cut(s)	19, 253, 500, 529, 930
HpySE526I	ACGT	1 cut(s)	826
Hsp92I	GRCGYC	1 cut(s)	826
Hsp92II	CATG	4 cut(s)	27, 143, 958, 1030
HspAI	GCGC	2 cut(s)	34, 250
Kpn2I	TCCGGA	1 cut(s)	742
Ksp22I	TGATCA	3 cut(s)	106, 355, 859
Kzo9I	GATC	7 cut(s)	106, 355, 448, 739, 859, 898, 925
LguI	GCTCTTC	1 cut(s)	151
LmnI	GCTCC	2 cut(s)	283, 515
Lsp1109I	GCAGC	4 cut(s)	273, 314, 530, 897
LweI	GCATC	3 cut(s)	589, 669, 995
MaeI	CTAG	1 cut(s)	711
MaeII	ACGT	1 cut(s)	826
MaeIII	GTNAC	2 cut(s)	194, 703
MalI	GATC	7 cut(s)	108, 357, 450, 741, 861, 900, 927
MboI	GATC	7 cut(s)	106, 355, 448, 739, 859, 898, 925
MboII	GAAGA	4 cut(s)	138, 177, 797, 916
MflI	RGATCY	1 cut(s)	925
MluCI	AATT	4 cut(s)	270, 320, 364, 462
MnlI	CCTC	9 cut(s)	126, 169, 212, 233, 253, 300, 501, 679, 1033
Mph1103I	ATGCAT	1 cut(s)	793
MroI	TCCGGA	1 cut(s)	742
MseI	TTAA	5 cut(s)	273, 297, 455, 465, 893
MslI	CAYNNNNRTG	3 cut(s)	138, 456, 991
MspA1I	CMGCKG	1 cut(s)	305
MspI	CCGG	2 cut(s)	406, 743
MspR9I	CCNGG	2 cut(s)	836, 983
MvaI	CCWGG	2 cut(s)	836, 983
MvnI	CGCG	1 cut(s)	231
MwoI	GCNNNNNNNGC	4 cut(s)	496, 515, 729, 983
NcoI	CCATGG	2 cut(s)	954, 1026
NdeII	GATC	7 cut(s)	106, 355, 448, 739, 859, 898, 925
NlaIII	CATG	4 cut(s)	27, 143, 958, 1030
NlaIV	GGNNCC	2 cut(s)	134, 440
NmuCI	GTSAC	2 cut(s)	194, 703
NsiI	ATGCAT	1 cut(s)	793
PciSI	GCTCTTC	1 cut(s)	151
PcsI	WCGNNNNNNNCGW	2 cut(s)	322, 997
PfeI	GAWTC	1 cut(s)	959
PinAI	ACCGGT	1 cut(s)	405
PkrI	GCNGC	5 cut(s)	230, 288, 304, 520, 912
PshBI	ATTAAT	1 cut(s)	465
PsiI	TTATAA	1 cut(s)	78
Psp6I	CCWGG	2 cut(s)	834, 981
PspFI	CCCAGC	1 cut(s)	934
PspGI	CCWGG	2 cut(s)	834, 981
PspN4I	GGNNCC	2 cut(s)	134, 440
PspPI	GGNCC	3 cut(s)	42, 838, 851
PstI	CTGCAG	3 cut(s)	432, 651, 1053
PsuI	RGATCY	1 cut(s)	925
PvuII	CAGCTG	1 cut(s)	305
RsaI	GTAC	2 cut(s)	717, 873
RsaNI	GTAC	2 cut(s)	716, 872
RseI	CAYNNNNRTG	3 cut(s)	138, 456, 991
SapI	GCTCTTC	1 cut(s)	151
SaqAI	TTAA	5 cut(s)	273, 297, 455, 465, 893
SatI	GCNGC	5 cut(s)	229, 287, 303, 519, 911
Sau3AI	GATC	7 cut(s)	106, 355, 448, 739, 859, 898, 925
Sau96I	GGNCC	3 cut(s)	42, 838, 851
ScaI	AGTACT	1 cut(s)	717
ScrFI	CCNGG	2 cut(s)	836, 983
SfaNI	GCATC	3 cut(s)	589, 669, 995
SfcI	CTRYAG	5 cut(s)	428, 647, 875, 963, 1049
SinI	GGWCC	3 cut(s)	42, 838, 851
SmiMI	CAYNNNNRTG	3 cut(s)	138, 456, 991
SmlI	CTYRAG	1 cut(s)	343
SmoI	CTYRAG	1 cut(s)	343
Sse9I	AATT	4 cut(s)	270, 320, 364, 462
SsiI	CCGC	1 cut(s)	229
SspMI	CTAG	1 cut(s)	711
StyD4I	CCNGG	2 cut(s)	834, 981
StyI	CCWWGG	4 cut(s)	68, 847, 954, 1026
TaaI	ACNGT	1 cut(s)	643
TaiI	ACGT	1 cut(s)	829
TaqI	TCGA	4 cut(s)	325, 545, 755, 940
TasI	AATT	4 cut(s)	270, 320, 364, 462
TatI	WGTACW	2 cut(s)	715, 871
TauI	GCSGC	1 cut(s)	231
TfiI	GAWTC	1 cut(s)	959
Tru1I	TTAA	5 cut(s)	273, 297, 455, 465, 893
Tru9I	TTAA	5 cut(s)	273, 297, 455, 465, 893
TscAI	CASTG	4 cut(s)	90, 156, 199, 1018
TseFI	GTSAC	2 cut(s)	194, 703
TseI	GCWGC	4 cut(s)	286, 302, 518, 910
Tsp45I	GTSAC	2 cut(s)	194, 703
TspDTI	ATGAA	3 cut(s)	177, 435, 936
TspGWI	ACGGA	1 cut(s)	324
TspRI	CASTG	4 cut(s)	90, 156, 199, 1018
VpaK11BI	GGWCC	3 cut(s)	42, 838, 851
VspI	ATTAAT	1 cut(s)	465
XapI	RAATTY	2 cut(s)	320, 364
XcmI	CCANNNNNNNNNTGG	1 cut(s)	90
XmiI	GTMKAC	2 cut(s)	336, 749
XspI	CTAG	1 cut(s)	711
ZraI	GACGTC	1 cut(s)	827
ZrmI	AGTACT	1 cut(s)	717
Zsp2I	ATGCAT	1 cut(s)	793

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.