Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0006475

Physical Location & Seq

Genomic Coordinates Forward (+)

8241155 .. 8247260

Gene Structure 6106 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1113400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2274 bp

ATGGCTGCTGGAAGACATGGGGACCATCGCGAAAATGAGATCAGGGGTCGAGAGTCCAGTTTTGATTTTCCAAGGAGGGATTTTGCTACGGCCAAAGAGGACAATGAAAGGATTAGGAACGGAAGTCCTGATGTTGAAATGGGTCAAGCTAGGGATTTGAGAGATAAAATTAGGGTTAGGCAGAAGGATATTAAGGAGGGGAATGCGGCTAATGGTGGGGATCGCTCCTCTTCGAGTAGGAATTATTCAAGCAATAGCAGTAGTCGGGTTGTGAGCCATGGGCCCAAGCCTTGTGGGTTTTCGGTTAGGGCATTGGATAGGGAACCTGGTGAATTGTCGAGTGAGAGTGGGTCTGACGATGCTATTGAGTCGGCAGCGCAGGTCAAAGAAAATACGGTTTCAAAAGTGGTGGAGAATGGAACTCAGTCTCCAAAAGAGAAGAAAAGGAAGTTTTCGCCAATTGTCTGGGATAGAGATGACAAAGGAGTGAGTAATCCATCAAAAAATAAGATCACCATGGCGGGTGCAGCAATTCCTCTACCTCCATCATTGTCTAGGGTACATCATCAGTCTCCTAATGTTATTTCAGATAGCAGTGTACAAGTATCTTCAGCTATAATTGATCAAGTCGAGAATTTACAATCTTCCTCACCAGTTAAGGCGCCAGTGGCGTCTGGGTATGTTAGTAATGGTGTCTCTGAGTCTCCACTAGGGATATCTTCACCAAATCAGCAATCGGGTAATGATCATGAAGCAGAGCAGCTAGAAGATGAAGAATATGTTCCCACCCGAAATATATCATCTTCAAGATGGGCAGCAGGAAATATATCACCTGATGAAGGTGAAAGTTTAGAGAACCAGGAAATGCCTAAAAGGAGGAAGAAAACTCCTCTGGTAGGGTCAGTGGAAGATAGAGTACAGAATAATTCATTAAACTCAGAGTCGGGGGAGCTTAAGAGAGACGAGTCCGAACTAGCAAGAGCGCAGTCATCTGAATCTGATGAAACAGATGCCCGTATAAGATCTTCCAGTGGGGATGATTACCAGGATAATGATTCAGAGAGGGATGACTATATGGATACTGACAAGGATCATGGTAATAATGGTTCTAGTGTTAGTCAGTCAGACACAGATTCAGATGATGATAATAACTCTCGCGAGACAGCAGAACCTGCAGCTCCTCCACAAAGAAGCGTGAACATGCTTCAGGGTTGTAGAAGTGTTGACGAGTTTGAGAGACTAAACAAGATCGATGAAGGCACTTATGGTGTTGTATATAGAGCTATGGATAAGAAAACTAAAGAAATTGTGGCCTTGAAGAAGGTAAAGATGGAGAAGGAAAGAGAAGGTTTCCCATTGACTTCTCTTAGGGAAATAAACATTCTTCTTTCGCTTCATCACCCATCAATTGTTGATGTGAAGGAAGTGGTGGTGGGGAGTAACCTTGATAGTATTTTTATGGTGATGGAGTACATGGAACATGATCTTAAAGCACTCATGGAGACAAAGAAGCAGCCATTCAGTCAGAGTGAAGTTAAATGCCTTATGCTTCAGTTATTGGCAGGTGTGAAATATCTTCATGATAATTGGGTGCTTCATCGAGATTTGAAGACATCTAACTTGCTTATGAATAACCGGGGTGAGTTGAAAATATGCGACTTTGGGATGGCTCGTCAGTATGGGAGTCCTTTAAAACCGTATACTCATTTGGTGGTTACACTTTGGTACAGGGCACCGGAACTTCTGTTGGGAGCGAAAGAATATTCAACATCTATCGATATGTGGTCACTTGGTTGTATCATGGCTGAACTATTATCAAAGGAGCCACTTTTCAATGGGAAAACTGAATTCGATCAACTTGACAAGATTTTTAGAATCCTTGGCACACCAAATGAGACAATCTGGCCCGGATATTCCAAGCTTCCTGGAGTGAAGGTCAACTTTGTTAAGCACCCGTATAATCTTTTGCGCAAGAAGTTCCCAGCTACGCCATTTACTGGATCTCCAGTACTTTCTGATGCTGGATTTAATTTGTTGAACAGGCTTCTAACTTATGATCCTGAAGAGAGAATTACAGCTGGAGATGCTCTGAATCATGAGTGGTTTCATGAAGTTCCTCTTCCTACAACGAAGGAGTTCATGCCTACCTTTCCTGCTCATCATGCTCAGGACAGGCGCACGCGGAGAGTTTTGAAAAGTCCAGATCCTTTGGAAGAGCAACGTAGAAAGGAGTTGCAGCAAGGGCAAATAGGAACTGGTGGAGTATTTGGCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007346 GO:0008150 GO:0008152 GO:0009987 GO:0010468 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051726 GO:0060255 GO:0065007 GO:0071704 GO:0097472 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

758

Amino Acids

84.53

Weight (kDa)

5.62

Isoelectric Point (pI)

53.08

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	411 - 702	2.7e-72	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	414 - 608	8.3e-35	Protein tyrosine and serine/threonine kinase
ABC1	PF03109	486 - 563	7.6e-06	ABC1 atypical kinase-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006475)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67580 AT1G67580
fragaria_vesca	FvH4_4g24200
malus_domestica	MD13G1113400.v1.1 MD16G1112700.v1.1
prunus_persica	Prupe.1G232700_v2.0.a1 Prupe.1G232700_v2.0.a1 Prupe.1G232700_v2.0.a1 Prupe.1G232700_v2.0.a1 Prupe.1G232700_v2.0.a1 Prupe.1G232700_v2.0.a1
pyrus_communis	pycom13g09880 pycom16g09530
rosa_chinensis	RchiOBHm_Chr4g0431041
rosa_laevigata	RLG00000006930
rosa_multiflora	Rmu_co8378327.1_g000001
rosa_roxburghii	Rroxscaffold_5G00372660
rosa_rugosa	Rorug04G0247500 Rorug04G0247600 Rorug04G0247700.1 Rorug04G0247800
rosa_samantha	Rh4AG304400 Rh4BG311600 Rh4CG327900 Rh4DG308100
rosa_wichuraiana	Rw4G026430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1553
Acc16I	TGCGCA	1 cut(s)	1970
Acc36I	ACCTGC	3 cut(s)	370, 1180, 1553
AccB1I	GGYRCC	2 cut(s)	661, 1732
AccB7I	CCANNNNNTGG	1 cut(s)	1997
AccI	GTMKAC	1 cut(s)	1700
AccII	CGCG	3 cut(s)	30, 1158, 2182
AciI	CCGC	3 cut(s)	206, 521, 2182
AclWI	GGATC	5 cut(s)	228, 1098, 2008, 2051, 2198
AcoI	YGGCCR	1 cut(s)	90
AcsI	RAATTY	2 cut(s)	634, 1847
AcuI	CTGAAG	4 cut(s)	594, 1190, 1535, 2082
AcyI	GRCGYC	2 cut(s)	662, 671
AfaI	GTAC	6 cut(s)	561, 600, 918, 1472, 1727, 2010
AfiI	CCNNNNNNNGG	3 cut(s)	839, 896, 1997
AflII	CTTAAG	1 cut(s)	953
AjnI	CCWGG	4 cut(s)	325, 858, 1044, 1924
AloI	GAACNNNNNNTCC	2 cut(s)	412, 444
AluBI	AGCT	9 cut(s)	149, 614, 763, 952, 1178, 1283, 1921, 1985, 2078
AluI	AGCT	9 cut(s)	149, 614, 763, 952, 1178, 1283, 1921, 1985, 2078
Alw26I	GTCTC	9 cut(s)	432, 576, 700, 708, 954, 1154, 1231, 1496, 1889
AlwI	GGATC	5 cut(s)	228, 1098, 2008, 2051, 2198
AlwNI	CAGNNNCTG	1 cut(s)	1172
AoxI	GGCC	4 cut(s)	90, 281, 1311, 1904
ApaI	GGGCCC	1 cut(s)	285
ApeKI	GCWGC	8 cut(s)	5, 374, 527, 760, 815, 1175, 1513, 2236
ApoI	RAATTY	2 cut(s)	634, 1847
Asp700I	GAANNNNTTC	2 cut(s)	780, 2135
AspLEI	GCGC	5 cut(s)	379, 664, 985, 1971, 2178
AspS9I	GGNCC	4 cut(s)	22, 281, 282, 1905
AsuC2I	CCSGG	2 cut(s)	1637, 1908
AsuHPI	GGTGA	9 cut(s)	341, 505, 642, 714, 822, 854, 1391, 1474, 1652
AvaII	GGWCC	1 cut(s)	22
BaeGI	GKGCMC	2 cut(s)	285, 1735
BanI	GGYRCC	2 cut(s)	661, 1732
BanII	GRGCYC	1 cut(s)	285
BarI	GAAGNNNNNNTAC	4 cut(s)	900, 932, 1009, 1041
BbsI	GAAGAC	2 cut(s)	19, 1616
BbvI	GCAGC	7 cut(s)	386, 539, 772, 827, 1187, 1525, 2248
BccI	CCATC	8 cut(s)	33, 505, 553, 804, 1324, 1411, 1459, 1660
BceAI	ACGGC	1 cut(s)	105
BciT130I	CCWGG	4 cut(s)	327, 860, 1046, 1926
BciVI	GTATCC	1 cut(s)	1072
BclI	TGATCA	2 cut(s)	622, 745
BcnI	CCSGG	2 cut(s)	1637, 1908
BcoDI	GTCTC	9 cut(s)	432, 576, 700, 708, 954, 1154, 1231, 1496, 1889
BfaI	CTAG	7 cut(s)	150, 555, 710, 764, 974, 1110, 2272
BfmI	CTRYAG	1 cut(s)	1173
BfoI	RGCGCY	1 cut(s)	665
BfrI	CTTAAG	1 cut(s)	953
BfuAI	ACCTGC	3 cut(s)	370, 1180, 1553
BfuI	GTATCC	1 cut(s)	1072
BglII	AGATCT	1 cut(s)	1022
BisI	GCNGC	9 cut(s)	6, 207, 375, 528, 761, 816, 1176, 1514, 2237
BlsI	GCNGC	9 cut(s)	7, 208, 376, 529, 762, 817, 1177, 1515, 2238
BmcAI	AGTACT	1 cut(s)	2010
Bme1390I	CCNGG	6 cut(s)	327, 860, 1046, 1637, 1908, 1926
Bme18I	GGWCC	1 cut(s)	22
BmgT120I	GGNCC	4 cut(s)	22, 281, 282, 1905
BmiI	GGNNCC	6 cut(s)	23, 283, 324, 663, 1734, 1824
BmrFI	CCNGG	6 cut(s)	327, 860, 1046, 1637, 1908, 1926
BmsI	GCATC	4 cut(s)	349, 1000, 2008, 2074
BpiI	GAAGAC	2 cut(s)	19, 1616
BpmI	CTGGAG	3 cut(s)	1947, 1989, 2100
Bpu10I	CCTNAGC	1 cut(s)	2166
BpuMI	CCSGG	2 cut(s)	1637, 1908
Bsa29I	ATCGAT	2 cut(s)	1251, 1776
BsaHI	GRCGYC	2 cut(s)	662, 671
BsaJI	CCNNGG	5 cut(s)	71, 277, 516, 1636, 1879
BsaWI	WCCGGW	1 cut(s)	1735
BsaXI	ACNNNNNCTCC	6 cut(s)	412, 442, 1920, 1950, 1987, 2017
Bsc4I	CCNNNNNNNGG	3 cut(s)	839, 896, 1997
Bse1I	ACTGG	7 cut(s)	57, 653, 665, 1029, 2002, 2006, 2260
BseBI	CCWGG	4 cut(s)	327, 860, 1046, 1926
BseCI	ATCGAT	2 cut(s)	1251, 1776
BseDI	CCNNGG	5 cut(s)	71, 277, 516, 1636, 1879
BseGI	GGATG	3 cut(s)	1042, 1072, 1671
BseLI	CCNNNNNNNGG	3 cut(s)	839, 896, 1997
BseMII	CTCAG	4 cut(s)	437, 690, 951, 2180
BseNI	ACTGG	7 cut(s)	57, 653, 665, 1029, 2002, 2006, 2260
BseRI	GAGGAG	3 cut(s)	217, 879, 1170
BseSI	GKGCMC	2 cut(s)	285, 1735
BseXI	GCAGC	7 cut(s)	386, 539, 772, 827, 1187, 1525, 2248
BseYI	CCCAGC	1 cut(s)	1981
BsgI	GTGCAG	1 cut(s)	546
Bsh1236I	CGCG	3 cut(s)	30, 1158, 2182
BshFI	GGCC	4 cut(s)	92, 283, 1313, 1906
BshNI	GGYRCC	2 cut(s)	661, 1732
BshVI	ATCGAT	2 cut(s)	1251, 1776
BsiSI	CCGG	3 cut(s)	1636, 1736, 1908
BslFI	GGGAC	1 cut(s)	35
BslI	CCNNNNNNNGG	3 cut(s)	839, 896, 1997
BsmAI	GTCTC	9 cut(s)	432, 576, 700, 708, 954, 1154, 1231, 1496, 1889
BsmBI	CGTCTC	1 cut(s)	954
BsmFI	GGGAC	1 cut(s)	35
BsmI	GAATGC	1 cut(s)	208
BsnI	GGCC	4 cut(s)	92, 283, 1313, 1906
Bsp120I	GGGCCC	1 cut(s)	281
Bsp1286I	GDGCHC	2 cut(s)	285, 1735
Bsp1407I	TGTACA	1 cut(s)	598
Bsp19I	CCATGG	2 cut(s)	277, 516
Bsp68I	TCGCGA	2 cut(s)	30, 1158
BspACI	CCGC	3 cut(s)	206, 521, 2182
BspANI	GGCC	4 cut(s)	92, 283, 1313, 1906
BspCNI	CTCAG	4 cut(s)	436, 691, 950, 2179
BspDI	ATCGAT	2 cut(s)	1251, 1776
BspFNI	CGCG	3 cut(s)	30, 1158, 2182
BspHI	TCATGA	4 cut(s)	748, 1579, 2095, 2107
BspLI	GGNNCC	6 cut(s)	23, 283, 324, 663, 1734, 1824
BspMAI	CTGCAG	1 cut(s)	1177
BspMI	ACCTGC	3 cut(s)	370, 1180, 1553
BspPI	GGATC	5 cut(s)	228, 1098, 2008, 2051, 2198
BspQI	GCTCTTC	1 cut(s)	2208
BspT107I	GGYRCC	2 cut(s)	661, 1732
BspTI	CTTAAG	1 cut(s)	953
BsrGI	TGTACA	1 cut(s)	598
BsrI	ACTGG	7 cut(s)	57, 653, 665, 1029, 2002, 2006, 2260
BssECI	CCNNGG	5 cut(s)	71, 277, 516, 1636, 1879
BssNAI	GTATAC	1 cut(s)	1701
BssNI	GRCGYC	2 cut(s)	662, 671
BssT1I	CCWWGG	4 cut(s)	71, 277, 516, 1879
Bst1107I	GTATAC	1 cut(s)	1701
Bst2UI	CCWGG	4 cut(s)	327, 860, 1046, 1926
Bst4CI	ACNGT	2 cut(s)	397, 1698
Bst6I	CTCTTC	4 cut(s)	235, 2058, 2124, 2208
BstACI	GRCGYC	2 cut(s)	662, 671
BstAFI	CTTAAG	1 cut(s)	953
BstAPI	GCANNNNNTGC	1 cut(s)	1172
BstAUI	TGTACA	1 cut(s)	598
BstC8I	GCNNGC	1 cut(s)	2180
BstDEI	CTNAG	5 cut(s)	423, 699, 937, 1367, 2166
BstDSI	CCRYGG	2 cut(s)	277, 516
BstENI	CCTNNNNNAGG	2 cut(s)	837, 894
BstF5I	GGATG	3 cut(s)	1042, 1072, 1671
BstFNI	CGCG	3 cut(s)	30, 1158, 2182
BstH2I	RGCGCY	1 cut(s)	665
BstHHI	GCGC	5 cut(s)	379, 664, 985, 1971, 2178
BstMAI	GTCTC	9 cut(s)	432, 576, 700, 708, 954, 1154, 1231, 1496, 1889
BstMWI	GCNNNNNNNGC	6 cut(s)	527, 668, 1172, 2084, 2162, 2242
BstNI	CCWGG	4 cut(s)	327, 860, 1046, 1926
BstNSI	RCATGY	1 cut(s)	1204
BstSCI	CCNGG	6 cut(s)	325, 858, 1044, 1635, 1906, 1924
BstSFI	CTRYAG	1 cut(s)	1173
BstSLI	GKGCMC	2 cut(s)	285, 1735
BstUI	CGCG	3 cut(s)	30, 1158, 2182
BstV1I	GCAGC	7 cut(s)	386, 539, 772, 827, 1187, 1525, 2248
BstV2I	GAAGAC	2 cut(s)	19, 1616
BstX2I	RGATCY	3 cut(s)	1022, 2000, 2203
BstXI	CCANNNNNNTGG	1 cut(s)	465
BstYI	RGATCY	3 cut(s)	1022, 2000, 2203
BstZ17I	GTATAC	1 cut(s)	1701
Bsu15I	ATCGAT	2 cut(s)	1251, 1776
BsuI	GTATCC	1 cut(s)	1072
BsuRI	GGCC	4 cut(s)	92, 283, 1313, 1906
BsuTUI	ATCGAT	2 cut(s)	1251, 1776
BtgI	CCRYGG	2 cut(s)	277, 516
BtgZI	GCGATG	1 cut(s)	11
BtsCI	GGATG	3 cut(s)	1042, 1072, 1671
BtsI	GCAGTG	1 cut(s)	601
BtsIMutI	CAGTG	4 cut(s)	601, 672, 909, 1036
BtuMI	TCGCGA	2 cut(s)	30, 1158
BveI	ACCTGC	3 cut(s)	370, 1180, 1553
Cac8I	GCNNGC	1 cut(s)	2180
CaiI	CAGNNNCTG	1 cut(s)	1172
CciI	TCATGA	4 cut(s)	748, 1579, 2095, 2107
CfoI	GCGC	5 cut(s)	379, 664, 985, 1971, 2178
Cfr13I	GGNCC	4 cut(s)	22, 281, 282, 1905
ClaI	ATCGAT	2 cut(s)	1251, 1776
CseI	GACGC	1 cut(s)	660
CsiI	ACCWGGT	1 cut(s)	325
Csp6I	GTAC	6 cut(s)	560, 599, 917, 1471, 1726, 2009
CspCI	CAANNNNNGTGG	4 cut(s)	274, 309, 390, 425
CviQI	GTAC	6 cut(s)	560, 599, 917, 1471, 1726, 2009
DdeI	CTNAG	5 cut(s)	423, 699, 937, 1367, 2166
DinI	GGCGCC	1 cut(s)	663
DraI	TTTAAA	1 cut(s)	1692
EaeI	YGGCCR	1 cut(s)	90
Eam1104I	CTCTTC	4 cut(s)	235, 2058, 2124, 2208
EarI	CTCTTC	4 cut(s)	235, 2058, 2124, 2208
Eco130I	CCWWGG	4 cut(s)	71, 277, 516, 1879
Eco24I	GRGCYC	1 cut(s)	285
Eco32I	GATATC	1 cut(s)	717
Eco47I	GGWCC	1 cut(s)	22
Eco57I	CTGAAG	4 cut(s)	594, 1190, 1535, 2082
EcoNI	CCTNNNNNAGG	2 cut(s)	837, 894
EcoRI	GAATTC	1 cut(s)	1847
EcoRII	CCWGG	4 cut(s)	325, 858, 1044, 1924
EcoRV	GATATC	1 cut(s)	717
EcoT14I	CCWWGG	4 cut(s)	71, 277, 516, 1879
EcoT38I	GRGCYC	1 cut(s)	285
EgeI	GGCGCC	1 cut(s)	663
EheI	GGCGCC	1 cut(s)	663
ErhI	CCWWGG	4 cut(s)	71, 277, 516, 1879
Esp3I	CGTCTC	1 cut(s)	954
FalI	AAGNNNNNCTT	4 cut(s)	1925, 1957, 2103, 2135
FaqI	GGGAC	1 cut(s)	35
FauI	CCCGC	1 cut(s)	514
FbaI	TGATCA	2 cut(s)	622, 745
FblI	GTMKAC	1 cut(s)	1700
Fnu4HI	GCNGC	9 cut(s)	6, 207, 375, 528, 761, 816, 1176, 1514, 2237
FokI	GGATG	3 cut(s)	1049, 1079, 1678
FriOI	GRGCYC	1 cut(s)	285
Fsp4HI	GCNGC	9 cut(s)	6, 207, 375, 528, 761, 816, 1176, 1514, 2237
FspBI	CTAG	7 cut(s)	150, 555, 710, 764, 974, 1110, 2272
FspI	TGCGCA	1 cut(s)	1970
GlaI	GCGC	5 cut(s)	378, 663, 984, 1970, 2177
GluI	GCNGC	9 cut(s)	6, 207, 375, 528, 761, 816, 1176, 1514, 2237
GsaI	CCCAGC	1 cut(s)	1985
GsuI	CTGGAG	3 cut(s)	1947, 1989, 2100
HaeII	RGCGCY	1 cut(s)	665
HaeIII	GGCC	4 cut(s)	92, 283, 1313, 1906
HapII	CCGG	3 cut(s)	1636, 1736, 1908
HgaI	GACGC	1 cut(s)	660
HhaI	GCGC	5 cut(s)	379, 664, 985, 1971, 2178
Hin1I	GRCGYC	2 cut(s)	662, 671
Hin6I	GCGC	5 cut(s)	377, 662, 983, 1969, 2176
HinP1I	GCGC	5 cut(s)	377, 662, 983, 1969, 2176
HincII	GTYRAC	2 cut(s)	1225, 1939
HindII	GTYRAC	2 cut(s)	1225, 1939
HindIII	AAGCTT	1 cut(s)	1919
HpaII	CCGG	3 cut(s)	1636, 1736, 1908
HphI	GGTGA	9 cut(s)	341, 505, 642, 714, 822, 854, 1391, 1474, 1652
Hpy166II	GTNNAC	5 cut(s)	599, 1198, 1225, 1701, 1939
Hpy8I	GTNNAC	5 cut(s)	599, 1198, 1225, 1701, 1939
HpyAV	CCTTC	9 cut(s)	178, 833, 1250, 1315, 1330, 1340, 1414, 1927, 2125
HpyCH4III	ACNGT	2 cut(s)	397, 1698
HpyCH4IV	ACGT	1 cut(s)	2221
HpyCH4V	TGCA	3 cut(s)	527, 1175, 2236
HpyF10VI	GCNNNNNNNGC	6 cut(s)	527, 668, 1172, 2084, 2162, 2242
HpyF3I	CTNAG	5 cut(s)	423, 699, 937, 1367, 2166
HpySE526I	ACGT	1 cut(s)	2221
Hsp92I	GRCGYC	2 cut(s)	662, 671
HspAI	GCGC	5 cut(s)	377, 662, 983, 1969, 2176
KasI	GGCGCC	1 cut(s)	661
Ksp22I	TGATCA	2 cut(s)	622, 745
LguI	GCTCTTC	1 cut(s)	2208
LmnI	GCTCC	5 cut(s)	230, 949, 1183, 1751, 1822
Lsp1109I	GCAGC	7 cut(s)	386, 539, 772, 827, 1187, 1525, 2248
LweI	GCATC	4 cut(s)	349, 1000, 2008, 2074
MabI	ACCWGGT	1 cut(s)	325
MaeI	CTAG	7 cut(s)	150, 555, 710, 764, 974, 1110, 2272
MaeII	ACGT	1 cut(s)	2221
MaeIII	GTNAC	3 cut(s)	1439, 1714, 1785
MfeI	CAATTG	2 cut(s)	459, 1407
MflI	RGATCY	3 cut(s)	1022, 2000, 2203
MhlI	GDGCHC	2 cut(s)	285, 1735
Mly113I	GGCGCC	1 cut(s)	662
MlyI	GAGTC	6 cut(s)	62, 377, 710, 950, 974, 1693
MroXI	GAANNNNTTC	2 cut(s)	780, 2135
MseI	TTAA	9 cut(s)	192, 657, 932, 954, 1488, 1536, 1691, 1947, 2028
MspA1I	CMGCKG	1 cut(s)	2078
MspCI	CTTAAG	1 cut(s)	953
MspI	CCGG	3 cut(s)	1636, 1736, 1908
MspR9I	CCNGG	6 cut(s)	327, 860, 1046, 1637, 1908, 1926
MunI	CAATTG	2 cut(s)	459, 1407
Mva1269I	GAATGC	1 cut(s)	208
MvaI	CCWGG	4 cut(s)	327, 860, 1046, 1926
MvnI	CGCG	3 cut(s)	30, 1158, 2182
MwoI	GCNNNNNNNGC	6 cut(s)	527, 668, 1172, 2084, 2162, 2242
NarI	GGCGCC	1 cut(s)	662
NciI	CCSGG	2 cut(s)	1637, 1908
NcoI	CCATGG	2 cut(s)	277, 516
NlaIV	GGNNCC	6 cut(s)	23, 283, 324, 663, 1734, 1824
NmuCI	GTSAC	1 cut(s)	1785
NruI	TCGCGA	2 cut(s)	30, 1158
NsbI	TGCGCA	1 cut(s)	1970
NspI	RCATGY	1 cut(s)	1204
PagI	TCATGA	4 cut(s)	748, 1579, 2095, 2107
PaqCI	CACCTGC	1 cut(s)	1553
PciSI	GCTCTTC	1 cut(s)	2208
PctI	GAATGC	1 cut(s)	208
PdmI	GAANNNNTTC	2 cut(s)	780, 2135
PfeI	GAWTC	5 cut(s)	995, 1055, 1133, 1875, 2092
PflMI	CCANNNNNTGG	1 cut(s)	1997
PfoI	TCCNGGA	1 cut(s)	1924
PkrI	GCNGC	9 cut(s)	7, 208, 376, 529, 762, 817, 1177, 1515, 2238
PleI	GAGTC	6 cut(s)	61, 376, 709, 949, 973, 1692
PluTI	GGCGCC	1 cut(s)	665
PpsI	GAGTC	6 cut(s)	61, 376, 709, 949, 973, 1692
Psp6I	CCWGG	4 cut(s)	325, 858, 1044, 1924
PspFI	CCCAGC	1 cut(s)	1981
PspGI	CCWGG	4 cut(s)	325, 858, 1044, 1924
PspN4I	GGNNCC	6 cut(s)	23, 283, 324, 663, 1734, 1824
PspOMI	GGGCCC	1 cut(s)	281
PspPI	GGNCC	4 cut(s)	22, 281, 282, 1905
PsrI	GAACNNNNNNTAC	2 cut(s)	1090, 1122
PstI	CTGCAG	1 cut(s)	1177
PstNI	CAGNNNCTG	1 cut(s)	1172
PsuI	RGATCY	3 cut(s)	1022, 2000, 2203
PvuII	CAGCTG	1 cut(s)	2078
RruI	TCGCGA	2 cut(s)	30, 1158
RsaI	GTAC	6 cut(s)	561, 600, 918, 1472, 1727, 2010
RsaNI	GTAC	6 cut(s)	560, 599, 917, 1471, 1726, 2009
SapI	GCTCTTC	1 cut(s)	2208
SaqAI	TTAA	9 cut(s)	192, 657, 932, 954, 1488, 1536, 1691, 1947, 2028
SatI	GCNGC	9 cut(s)	6, 207, 375, 528, 761, 816, 1176, 1514, 2237
Sau96I	GGNCC	4 cut(s)	22, 281, 282, 1905
ScaI	AGTACT	1 cut(s)	2010
SchI	GAGTC	6 cut(s)	62, 377, 710, 950, 974, 1693
ScrFI	CCNGG	6 cut(s)	327, 860, 1046, 1637, 1908, 1926
SduI	GDGCHC	2 cut(s)	285, 1735
SexAI	ACCWGGT	1 cut(s)	325
SfaNI	GCATC	4 cut(s)	349, 1000, 2008, 2074
SfcI	CTRYAG	1 cut(s)	1173
SfoI	GGCGCC	1 cut(s)	663
SinI	GGWCC	1 cut(s)	22
SmlI	CTYRAG	1 cut(s)	953
SmoI	CTYRAG	1 cut(s)	953
SsiI	CCGC	3 cut(s)	206, 521, 2182
SspDI	GGCGCC	1 cut(s)	661
SspI	AATATT	1 cut(s)	1763
SspMI	CTAG	7 cut(s)	150, 555, 710, 764, 974, 1110, 2272
StyD4I	CCNGG	6 cut(s)	325, 858, 1044, 1635, 1906, 1924
StyI	CCWWGG	4 cut(s)	71, 277, 516, 1879
TaaI	ACNGT	2 cut(s)	397, 1698
TaiI	ACGT	1 cut(s)	2224
TaqI	TCGA	8 cut(s)	49, 233, 338, 630, 1251, 1600, 1776, 1851
TatI	WGTACW	4 cut(s)	598, 916, 1470, 2008
TauI	GCSGC	1 cut(s)	209
TfiI	GAWTC	5 cut(s)	995, 1055, 1133, 1875, 2092
Tru1I	TTAA	9 cut(s)	192, 657, 932, 954, 1488, 1536, 1691, 1947, 2028
Tru9I	TTAA	9 cut(s)	192, 657, 932, 954, 1488, 1536, 1691, 1947, 2028
TscAI	CASTG	4 cut(s)	601, 672, 909, 1036
TseFI	GTSAC	1 cut(s)	1785
TseI	GCWGC	8 cut(s)	5, 374, 527, 760, 815, 1175, 1513, 2236
Tsp45I	GTSAC	1 cut(s)	1785
TspGWI	ACGGA	1 cut(s)	135
TspRI	CASTG	4 cut(s)	601, 672, 909, 1036
Van91I	CCANNNNNTGG	1 cut(s)	1997
Vha464I	CTTAAG	1 cut(s)	953
VpaK11BI	GGWCC	1 cut(s)	22
XagI	CCTNNNNNAGG	2 cut(s)	837, 894
XapI	RAATTY	2 cut(s)	634, 1847
XceI	RCATGY	1 cut(s)	1204
XmiI	GTMKAC	1 cut(s)	1700
XmnI	GAANNNNTTC	2 cut(s)	780, 2135
XspI	CTAG	7 cut(s)	150, 555, 710, 764, 974, 1110, 2272
ZrmI	AGTACT	1 cut(s)	2010

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.