Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4A

Orthogroup ID

OG0006475

Physical Location & Seq

Genomic Coordinates Reverse (-)

61645422 .. 61652276

Gene Structure 6855 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4AG304400.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2433 bp

ATGGCGGCTGGAAGACATGGGGGTTATCGCGAAAACGAGTTCAGGGAGCGAGAGTCCAGTTTCGAATTGCCGCGGAGGGATTTAGCTTCTGCCAAGGAGGACTTGGAGCGGATCGGGAATGGGAATCGTGATGTTCACAAGGGCCGCGTTCGGGATTTGAGAGATAGAATCAGGGCTAGGTCCAAGGAGAGGGCAGTGGCTAATGGTGGATACCGATCCTCTTCGAGTAGGAGTGATTCCGGCAGCAGTGGCGGTGGTGGTGGGCTAGTAGTAGCCGGTCGCGGGCCTAAGCCGTGTGGGTTTTCTGCCAAGGCATTGGATAGGGAACCTGGTGAATTGTCAAGTGAGAGCGGGTCTGATGATGCTATTGAATCCGGGTCGCAGGGCAGAGATGTAGAGGTTATGAAGGTGGTGGAGAATGGAAGTCAGACTCCCAAGGAGAGGAAAAGGAAGTTTTCGCCTATTGTGTGGGATAGAGATGATAAAGAAGGGAGTAATTCGTCCAGGACTAGGATTACAACAGTGGCAACAGCTCTTCCTCCTCCACCCCCACTGGCTAGGGTGTCTCGTCAGTCTCCTAATGTTACTTCAGATAGCAGGGTACAAATATCTTCAGCTAAAATTGATGAAGCCGACAATTTACAACCTTCCTTTCCGGTTAAGTCGTCTCCTGTGGCACCTGAGACTGTTAGCAATGGTGTATATGAGTCTCCGGTAGAGTTGTCTTCACCAGATCAGCATTTAGGGGATGTTCGTGAAGCAGAGCAGCTAGAAGATGAAGATTATGTTCCAACCCGAAATATATCATCTTCAAGATGGGCAGCAGGAAATAATTCTCCTGGTGATGAAGGTGGAATTTCAGAAAACGAGGAAATTCCTAAAAGGAGGAGGAAAATGCCTCGTTTAGGATCAGTGGAAAACAGAGTACGCAACAGCTCATTGACTCCGGAGCTAGGGGAGCTTAAGAAAGATGGGTCTGAACGAGGTAGGGCGAGATCATCCGAGTCTGATGAACGAGATGCCCTTCGAAGGTCTAGTGGGGATGACTATCCTGATAATGACTCAGAGAGGGATGACTACATGGATGTTGACAAGGATAATGGTAATAATTATAGTGTTAGTCGTTATGATACTAGTTCAGAGGATGAGCATGATTCTCCTGAGACACCAGAGCCTGCAGCTCCTCCACAAAGAAGTGTAAACATGCTTCAGGGTTGTAGAAGTGTTGATGAGTTCGAGAGACTAAACAAGATAGATGAGGGCACTTATGGTGTTGTATATCGGGCCCAAGATAAGAAAACTGGTGAAATTGTGGCATTGAAGAAGGTAAAGATGGAGAAGGAAAGAGAAGGTTTTCCGTTAACTTCTCTGAGGGAAATAAACATTCTCCTTTCTTTTCATCATCCATCAATTGTCGATGTTAAGGAAGTAGTGGTGGGCAGTAACCTTGATAGCATTTTTATGGTGATGGAATACATGGAGCATGATCTTAAAGCACTCATGGAGACAATGAAGCAGCCATTTAGTCCGAGTGAAGTCAAATGCCTTATGCTCCAGCTATTGGAAGGTGTCAAATATCTTCATGATAATTGGGTGCTTCATCGGGATTTGAAGACATCTAACCTGCTTTTGAATAATCGGGGTGAGTTGAAGATCTGTGACTTTGGGTTGGCTCGTCAATATGGAAGTCCTTTGAAACCGTATACTCATTTGGTGGTTACTCTTTGGTACAGGGCGCCGGAACTTCTGTTGGGAGCAAGAGAATATTCAACGGCAATCGATATGTGGTCACTAGGTTGTATCATGGCTGAGCTGTTATCAAAGGAGCCGCTTTTTAATGGGAAAACTGAATTTGAACAACTTGACAAAATTTTTAAGATACTTGGCACACCAAATGAGACTATTTGGCCTGGGTTTTCCAAGCTTCCTGGAGTAAAGGTCAACTTTGTTAAGCATCAGTATAACCTTTTGCGCAAGAAGTTCCCAGCTACTTCATTTACTGGATCTCCAGTTCTTACTGAAGCTGGATTTGATTTGTTGAATAAGCTTCTAACTTATGACCCTGAGAAGAGAATTTCTGCTGATGCTGCTCTGAATCATGAGTGGTTTCGCGAAGTCCCTCTTCCCAAGACGAAAGATTTCATGCCCACGTTTCCTGCTCAACATGCTCAAGACAGGCGCTCACGGAGAATGTTGAAGAGTCCAGATCCCTTGGAAGAGCAACGGAGAAAGGAGTTGCAAGGGGGAACTTATAGAACTTCAATTAGTTGTTCCACAGGCTCACTAGAGAAGAATTTTAAGAAATACGCTAAAAGGCAAAAAGATTCCGGTCTCTGTTCCTGGACACTTCGCATTCTTAACATTGACAAAATGTCAGGTTACAAAATGTTCATCCAGACATTGGTGGAAAACGCTCAATTGTGCAAGTTCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0004693 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007346 GO:0008150 GO:0008152 GO:0009987 GO:0010468 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019222 GO:0019538 GO:0036211 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051726 GO:0060255 GO:0065007 GO:0071704 GO:0097472 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

810

Amino Acids

91.03

Weight (kDa)

6.73

Isoelectric Point (pI)

55.67

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	412 - 703	2.5e-72	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	415 - 610	1.3e-34	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006475)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G67580 AT1G67580
fragaria_vesca	FvH4_4g24200
malus_domestica	MD13G1113400.v1.1 MD16G1112700.v1.1
prunus_persica	Prupe.1G232700_v2.0.a1 Prupe.1G232700_v2.0.a1 Prupe.1G232700_v2.0.a1 Prupe.1G232700_v2.0.a1 Prupe.1G232700_v2.0.a1 Prupe.1G232700_v2.0.a1
pyrus_communis	pycom13g09880 pycom16g09530
rosa_chinensis	RchiOBHm_Chr4g0431041
rosa_laevigata	RLG00000006930
rosa_multiflora	Rmu_co8378327.1_g000001
rosa_roxburghii	Rroxscaffold_5G00372660
rosa_rugosa	Rorug04G0247500 Rorug04G0247600 Rorug04G0247700.1 Rorug04G0247800
rosa_samantha	Rh4AG304400 Rh4BG311600 Rh4CG327900 Rh4DG308100
rosa_wichuraiana	Rw4G026430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	1973
Acc36I	ACCTGC	1 cut(s)	1632
AccB1I	GGYRCC	2 cut(s)	676, 1735
AccB7I	CCANNNNNTGG	2 cut(s)	1561, 2401
AccBSI	CCGCTC	2 cut(s)	109, 351
AccI	GTMKAC	1 cut(s)	1703
AccII	CGCG	5 cut(s)	30, 73, 147, 282, 2112
AccIII	TCCGGA	1 cut(s)	946
AciI	CCGC	9 cut(s)	5, 71, 73, 109, 145, 252, 282, 351, 1829
AclWI	GGATC	5 cut(s)	119, 210, 916, 2011, 2201
AcsI	RAATTY	6 cut(s)	855, 873, 1850, 1869, 2073, 2293
AcuI	CTGAAG	4 cut(s)	573, 597, 1193, 2040
AcyI	GRCGYC	1 cut(s)	1736
AfaI	GTAC	3 cut(s)	603, 927, 1730
AflII	CTTAAG	1 cut(s)	962
AhdI	GACNNNNNGTC	1 cut(s)	2371
AhlI	ACTAGT	1 cut(s)	1133
AjnI	CCWGG	6 cut(s)	328, 503, 838, 1909, 1927, 2339
AloI	GAACNNNNNNTCC	2 cut(s)	1995, 2027
AlwI	GGATC	5 cut(s)	119, 210, 916, 2011, 2201
AlwNI	CAGNNNCTG	1 cut(s)	1175
Aor13HI	TCCGGA	1 cut(s)	946
AoxI	GGCC	4 cut(s)	142, 284, 1284, 1907
ApaI	GGGCCC	1 cut(s)	1288
ApeKI	GCWGC	6 cut(s)	243, 766, 821, 1178, 1516, 2087
ApoI	RAATTY	6 cut(s)	855, 873, 1850, 1869, 2073, 2293
Asp700I	GAANNNNTTC	3 cut(s)	832, 1353, 2138
AspLEI	GCGC	3 cut(s)	1738, 1974, 2181
AspS9I	GGNCC	5 cut(s)	142, 180, 284, 1284, 1285
AsuC2I	CCSGG	1 cut(s)	376
AsuHPI	GGTGA	6 cut(s)	344, 720, 854, 1316, 1477, 1655
AsuII	TTCGAA	2 cut(s)	63, 1027
AvaII	GGWCC	1 cut(s)	180
BaeGI	GKGCMC	2 cut(s)	1265, 1288
BanI	GGYRCC	2 cut(s)	676, 1735
BanII	GRGCYC	1 cut(s)	1288
BarI	GAAGNNNNNNTAC	2 cut(s)	1191, 1223
BbsI	GAAGAC	3 cut(s)	19, 717, 1619
BbvI	GCAGC	6 cut(s)	255, 778, 833, 1190, 1528, 2074
BccI	CCATC	5 cut(s)	810, 965, 1327, 1414, 1462
BceAI	ACGGC	2 cut(s)	277, 1788
BciT130I	CCWGG	6 cut(s)	330, 505, 840, 1911, 1929, 2341
BciVI	GTATCC	1 cut(s)	203
BcnI	CCSGG	1 cut(s)	376
BcuI	ACTAGT	1 cut(s)	1133
BfmI	CTRYAG	1 cut(s)	1176
BfoI	RGCGCY	2 cut(s)	1739, 2182
BfrI	CTTAAG	1 cut(s)	962
BfuAI	ACCTGC	1 cut(s)	1632
BfuI	GTATCC	1 cut(s)	203
BglII	AGATCT	1 cut(s)	1653
BlpI	GCTNAGC	1 cut(s)	1809
Bme1390I	CCNGG	7 cut(s)	330, 376, 505, 840, 1911, 1929, 2341
Bme18I	GGWCC	1 cut(s)	180
BmeRI	GACNNNNNGTC	1 cut(s)	2371
BmgT120I	GGNCC	5 cut(s)	142, 180, 284, 1284, 1285
BmiI	GGNNCC	5 cut(s)	327, 678, 1286, 1737, 1827
BmrFI	CCNGG	7 cut(s)	330, 376, 505, 840, 1911, 1929, 2341
BmsI	GCATC	4 cut(s)	352, 1009, 1963, 2074
BpiI	GAAGAC	3 cut(s)	19, 717, 1619
BpmI	CTGGAG	3 cut(s)	1538, 1950, 1992
Bpu10I	CCTNAGC	1 cut(s)	288
Bpu1102I	GCTNAGC	1 cut(s)	1809
Bpu14I	TTCGAA	2 cut(s)	63, 1027
BpuEI	CTTGAG	1 cut(s)	2154
BpuMI	CCSGG	1 cut(s)	376
Bsa29I	ATCGAT	1 cut(s)	1779
BsaBI	GATNNNNATC	2 cut(s)	214, 1047
BsaHI	GRCGYC	1 cut(s)	1736
BsaI	GGTCTC	1 cut(s)	2336
BsaJI	CCNNGG	7 cut(s)	71, 93, 183, 309, 435, 1910, 2211
BsaWI	WCCGGW	4 cut(s)	655, 712, 946, 2327
BsaXI	ACNNNNNCTCC	6 cut(s)	38, 68, 1923, 1953, 1990, 2020
Bse118I	RCCGGY	1 cut(s)	275
Bse1I	ACTGG	5 cut(s)	57, 558, 1306, 2005, 2009
Bse3DI	GCAATG	1 cut(s)	700
Bse8I	GATNNNNATC	2 cut(s)	214, 1047
BseAI	TCCGGA	1 cut(s)	946
BseBI	CCWGG	6 cut(s)	330, 505, 840, 1911, 1929, 2341
BseCI	ATCGAT	1 cut(s)	1779
BseDI	CCNNGG	7 cut(s)	71, 93, 183, 309, 435, 1910, 2211
BseGI	GGATG	8 cut(s)	754, 998, 1048, 1078, 1090, 1150, 1402, 2391
BseJI	GATNNNNATC	2 cut(s)	214, 1047
BseMI	GCAATG	1 cut(s)	700
BseMII	CTCAG	6 cut(s)	672, 1077, 1152, 1361, 1800, 2055
BseNI	ACTGG	5 cut(s)	57, 558, 1306, 2005, 2009
BseRI	GAGGAG	3 cut(s)	531, 901, 1173
BseSI	GKGCMC	2 cut(s)	1265, 1288
BseXI	GCAGC	6 cut(s)	255, 778, 833, 1190, 1528, 2074
BseYI	CCCAGC	1 cut(s)	1984
Bsh1236I	CGCG	5 cut(s)	30, 73, 147, 282, 2112
Bsh1285I	CGRYCG	1 cut(s)	280
BshFI	GGCC	4 cut(s)	144, 286, 1286, 1909
BshNI	GGYRCC	2 cut(s)	676, 1735
BshVI	ATCGAT	1 cut(s)	1779
BsiEI	CGRYCG	1 cut(s)	280
BsiSI	CCGG	8 cut(s)	240, 276, 375, 656, 713, 947, 1739, 2328
BslFI	GGGAC	1 cut(s)	2102
BsmBI	CGTCTC	1 cut(s)	672
BsmFI	GGGAC	1 cut(s)	2102
BsmI	GAATGC	1 cut(s)	2352
BsnI	GGCC	4 cut(s)	144, 286, 1286, 1909
Bso31I	GGTCTC	1 cut(s)	2336
Bsp119I	TTCGAA	2 cut(s)	63, 1027
Bsp120I	GGGCCC	1 cut(s)	1284
Bsp1286I	GDGCHC	2 cut(s)	1265, 1288
Bsp13I	TCCGGA	1 cut(s)	946
Bsp143I	GATC	9 cut(s)	111, 215, 733, 908, 995, 1486, 1653, 2003, 2206
Bsp1720I	GCTNAGC	1 cut(s)	1809
Bsp68I	TCGCGA	2 cut(s)	30, 2112
BspACI	CCGC	9 cut(s)	5, 71, 73, 109, 145, 252, 282, 351, 1829
BspANI	GGCC	4 cut(s)	144, 286, 1286, 1909
BspCNI	CTCAG	6 cut(s)	673, 1076, 1153, 1362, 1801, 2056
BspDI	ATCGAT	1 cut(s)	1779
BspEI	TCCGGA	1 cut(s)	946
BspFNI	CGCG	5 cut(s)	30, 73, 147, 282, 2112
BspHI	TCATGA	2 cut(s)	1582, 2098
BspLI	GGNNCC	5 cut(s)	327, 678, 1286, 1737, 1827
BspMAI	CTGCAG	1 cut(s)	1180
BspMI	ACCTGC	1 cut(s)	1632
BspPI	GGATC	5 cut(s)	119, 210, 916, 2011, 2201
BspQI	GCTCTTC	2 cut(s)	540, 2211
BspT104I	TTCGAA	2 cut(s)	63, 1027
BspT107I	GGYRCC	2 cut(s)	676, 1735
BspTI	CTTAAG	1 cut(s)	962
BspTNI	GGTCTC	1 cut(s)	2336
BsrBI	CCGCTC	2 cut(s)	109, 351
BsrDI	GCAATG	1 cut(s)	700
BsrFI	RCCGGY	1 cut(s)	275
BsrI	ACTGG	5 cut(s)	57, 558, 1306, 2005, 2009
BssAI	RCCGGY	1 cut(s)	275
BssECI	CCNNGG	7 cut(s)	71, 93, 183, 309, 435, 1910, 2211
BssMI	GATC	9 cut(s)	111, 215, 733, 908, 995, 1486, 1653, 2003, 2206
BssNAI	GTATAC	1 cut(s)	1704
BssNI	GRCGYC	1 cut(s)	1736
BssT1I	CCWWGG	5 cut(s)	93, 183, 309, 435, 2211
Bst1107I	GTATAC	1 cut(s)	1704
Bst2UI	CCWGG	6 cut(s)	330, 505, 840, 1911, 1929, 2341
Bst4CI	ACNGT	3 cut(s)	523, 688, 1701
Bst6I	CTCTTC	6 cut(s)	226, 540, 2063, 2127, 2192, 2211
BstACI	GRCGYC	1 cut(s)	1736
BstAFI	CTTAAG	1 cut(s)	962
BstBI	TTCGAA	2 cut(s)	63, 1027
BstC8I	GCNNGC	2 cut(s)	284, 1176
BstDEI	CTNAG	7 cut(s)	288, 681, 1063, 1161, 1370, 1809, 2064
BstDSI	CCRYGG	1 cut(s)	71
BstENI	CCTNNNNNAGG	1 cut(s)	903
BstF5I	GGATG	8 cut(s)	754, 998, 1048, 1078, 1090, 1150, 1402, 2391
BstFNI	CGCG	5 cut(s)	30, 73, 147, 282, 2112
BstH2I	RGCGCY	2 cut(s)	1739, 2182
BstHHI	GCGC	3 cut(s)	1738, 1974, 2181
BstKTI	GATC	9 cut(s)	114, 218, 736, 911, 998, 1489, 1656, 2006, 2209
BstMBI	GATC	9 cut(s)	111, 215, 733, 908, 995, 1486, 1653, 2003, 2206
BstMCI	CGRYCG	1 cut(s)	280
BstMWI	GCNNNNNNNGC	4 cut(s)	249, 958, 2087, 2165
BstNI	CCWGG	6 cut(s)	330, 505, 840, 1911, 1929, 2341
BstNSI	RCATGY	2 cut(s)	1207, 2168
BstSCI	CCNGG	7 cut(s)	328, 374, 503, 838, 1909, 1927, 2339
BstSFI	CTRYAG	1 cut(s)	1176
BstSLI	GKGCMC	2 cut(s)	1265, 1288
BstUI	CGCG	5 cut(s)	30, 73, 147, 282, 2112
BstV1I	GCAGC	6 cut(s)	255, 778, 833, 1190, 1528, 2074
BstV2I	GAAGAC	3 cut(s)	19, 717, 1619
BstX2I	RGATCY	3 cut(s)	1653, 2003, 2206
BstXI	CCANNNNNNTGG	1 cut(s)	316
BstYI	RGATCY	3 cut(s)	1653, 2003, 2206
BstZ17I	GTATAC	1 cut(s)	1704
Bsu15I	ATCGAT	1 cut(s)	1779
BsuI	GTATCC	1 cut(s)	203
BsuRI	GGCC	4 cut(s)	144, 286, 1286, 1909
BsuTUI	ATCGAT	1 cut(s)	1779
BtgI	CCRYGG	1 cut(s)	71
BtsCI	GGATG	8 cut(s)	754, 998, 1048, 1078, 1090, 1150, 1402, 2391
BtsI	GCAGTG	2 cut(s)	201, 253
BtsIMutI	CAGTG	5 cut(s)	201, 253, 528, 551, 918
BtuMI	TCGCGA	2 cut(s)	30, 2112
BveI	ACCTGC	1 cut(s)	1632
Cac8I	GCNNGC	2 cut(s)	284, 1176
CaiI	CAGNNNCTG	1 cut(s)	1175
CciI	TCATGA	2 cut(s)	1582, 2098
CfoI	GCGC	3 cut(s)	1738, 1974, 2181
Cfr10I	RCCGGY	1 cut(s)	275
Cfr13I	GGNCC	5 cut(s)	142, 180, 284, 1284, 1285
Cfr42I	CCGCGG	1 cut(s)	74
ClaI	ATCGAT	1 cut(s)	1779
CsiI	ACCWGGT	1 cut(s)	328
Csp6I	GTAC	3 cut(s)	602, 926, 1729
CviQI	GTAC	3 cut(s)	602, 926, 1729
DdeI	CTNAG	7 cut(s)	288, 681, 1063, 1161, 1370, 1809, 2064
DinI	GGCGCC	1 cut(s)	1737
DpnI	GATC	9 cut(s)	113, 217, 735, 910, 997, 1488, 1655, 2005, 2208
DpnII	GATC	9 cut(s)	111, 215, 733, 908, 995, 1486, 1653, 2003, 2206
DriI	GACNNNNNGTC	1 cut(s)	2371
Eam1104I	CTCTTC	6 cut(s)	226, 540, 2063, 2127, 2192, 2211
Eam1105I	GACNNNNNGTC	1 cut(s)	2371
EarI	CTCTTC	6 cut(s)	226, 540, 2063, 2127, 2192, 2211
Eco130I	CCWWGG	5 cut(s)	93, 183, 309, 435, 2211
Eco24I	GRGCYC	1 cut(s)	1288
Eco31I	GGTCTC	1 cut(s)	2336
Eco47I	GGWCC	1 cut(s)	180
Eco57I	CTGAAG	4 cut(s)	573, 597, 1193, 2040
EcoNI	CCTNNNNNAGG	1 cut(s)	903
EcoRII	CCWGG	6 cut(s)	328, 503, 838, 1909, 1927, 2339
EcoT14I	CCWWGG	5 cut(s)	93, 183, 309, 435, 2211
EcoT38I	GRGCYC	1 cut(s)	1288
EgeI	GGCGCC	1 cut(s)	1737
EheI	GGCGCC	1 cut(s)	1737
ErhI	CCWWGG	5 cut(s)	93, 183, 309, 435, 2211
Esp3I	CGTCTC	1 cut(s)	672
FalI	AAGNNNNNCTT	6 cut(s)	86, 118, 1928, 1960, 2106, 2138
FaqI	GGGAC	1 cut(s)	2102
FauI	CCCGC	2 cut(s)	275, 344
FblI	GTMKAC	1 cut(s)	1703
FokI	GGATG	8 cut(s)	761, 985, 1055, 1085, 1097, 1157, 1389, 2378
FriOI	GRGCYC	1 cut(s)	1288
FspI	TGCGCA	1 cut(s)	1973
GlaI	GCGC	3 cut(s)	1737, 1973, 2180
GsaI	CCCAGC	1 cut(s)	1988
GsuI	CTGGAG	3 cut(s)	1538, 1950, 1992
HaeII	RGCGCY	2 cut(s)	1739, 2182
HaeIII	GGCC	4 cut(s)	144, 286, 1286, 1909
HapII	CCGG	8 cut(s)	240, 276, 375, 656, 713, 947, 1739, 2328
HhaI	GCGC	3 cut(s)	1738, 1974, 2181
Hin1I	GRCGYC	1 cut(s)	1736
Hin6I	GCGC	3 cut(s)	1736, 1972, 2179
HinP1I	GCGC	3 cut(s)	1736, 1972, 2179
HincII	GTYRAC	3 cut(s)	1090, 1362, 1942
HindII	GTYRAC	3 cut(s)	1090, 1362, 1942
HindIII	AAGCTT	2 cut(s)	1922, 2045
HpaI	GTTAAC	1 cut(s)	1362
HpaII	CCGG	8 cut(s)	240, 276, 375, 656, 713, 947, 1739, 2328
HphI	GGTGA	6 cut(s)	344, 720, 854, 1316, 1477, 1655
Hpy166II	GTNNAC	6 cut(s)	136, 1090, 1201, 1362, 1704, 1942
Hpy8I	GTNNAC	6 cut(s)	136, 1090, 1201, 1362, 1704, 1942
HpyCH4III	ACNGT	3 cut(s)	523, 688, 1701
HpyCH4IV	ACGT	1 cut(s)	2150
HpyCH4V	TGCA	3 cut(s)	1178, 2239, 2424
HpyF10VI	GCNNNNNNNGC	4 cut(s)	249, 958, 2087, 2165
HpyF3I	CTNAG	7 cut(s)	288, 681, 1063, 1161, 1370, 1809, 2064
HpySE526I	ACGT	1 cut(s)	2150
Hsp92I	GRCGYC	1 cut(s)	1736
HspAI	GCGC	3 cut(s)	1736, 1972, 2179
KasI	GGCGCC	1 cut(s)	1735
Kpn2I	TCCGGA	1 cut(s)	946
KspAI	GTTAAC	1 cut(s)	1362
KspI	CCGCGG	1 cut(s)	74
Kzo9I	GATC	9 cut(s)	111, 215, 733, 908, 995, 1486, 1653, 2003, 2206
LguI	GCTCTTC	2 cut(s)	540, 2211
LmnI	GCTCC	9 cut(s)	46, 106, 949, 958, 1186, 1480, 1557, 1754, 1825
Lsp1109I	GCAGC	6 cut(s)	255, 778, 833, 1190, 1528, 2074
LweI	GCATC	4 cut(s)	352, 1009, 1963, 2074
MabI	ACCWGGT	1 cut(s)	328
MaeII	ACGT	1 cut(s)	2150
MaeIII	GTNAC	6 cut(s)	583, 1442, 1658, 1717, 1788, 2378
MalI	GATC	9 cut(s)	113, 217, 735, 910, 997, 1488, 1655, 2005, 2208
MbiI	CCGCTC	2 cut(s)	109, 351
MboI	GATC	9 cut(s)	111, 215, 733, 908, 995, 1486, 1653, 2003, 2206
MfeI	CAATTG	2 cut(s)	1410, 2417
MflI	RGATCY	3 cut(s)	1653, 2003, 2206
MhlI	GDGCHC	2 cut(s)	1265, 1288
Mly113I	GGCGCC	1 cut(s)	1736
MlyI	GAGTC	7 cut(s)	62, 424, 716, 937, 1013, 1055, 2209
MmeI	TCCRAC	1 cut(s)	815
MroI	TCCGGA	1 cut(s)	946
MroXI	GAANNNNTTC	3 cut(s)	832, 1353, 2138
MslI	CAYNNNNRTG	1 cut(s)	1460
MspA1I	CMGCKG	1 cut(s)	73
MspCI	CTTAAG	1 cut(s)	962
MspI	CCGG	8 cut(s)	240, 276, 375, 656, 713, 947, 1739, 2328
MspR9I	CCNGG	7 cut(s)	330, 376, 505, 840, 1911, 1929, 2341
MunI	CAATTG	2 cut(s)	1410, 2417
Mva1269I	GAATGC	1 cut(s)	2352
MvaI	CCWGG	6 cut(s)	330, 505, 840, 1911, 1929, 2341
MvnI	CGCG	5 cut(s)	30, 73, 147, 282, 2112
MwoI	GCNNNNNNNGC	4 cut(s)	249, 958, 2087, 2165
NarI	GGCGCC	1 cut(s)	1736
NciI	CCSGG	1 cut(s)	376
NdeII	GATC	9 cut(s)	111, 215, 733, 908, 995, 1486, 1653, 2003, 2206
NlaIV	GGNNCC	5 cut(s)	327, 678, 1286, 1737, 1827
NmuCI	GTSAC	2 cut(s)	1658, 1788
NruI	TCGCGA	2 cut(s)	30, 2112
NsbI	TGCGCA	1 cut(s)	1973
NspI	RCATGY	2 cut(s)	1207, 2168
NspV	TTCGAA	2 cut(s)	63, 1027
PagI	TCATGA	2 cut(s)	1582, 2098
PciSI	GCTCTTC	2 cut(s)	540, 2211
PctI	GAATGC	1 cut(s)	2352
PdmI	GAANNNNTTC	3 cut(s)	832, 1353, 2138
PfeI	GAWTC	7 cut(s)	124, 168, 236, 371, 1154, 2095, 2324
PflMI	CCANNNNNTGG	2 cut(s)	1561, 2401
PfoI	TCCNGGA	3 cut(s)	503, 1927, 2339
PleI	GAGTC	7 cut(s)	61, 424, 715, 937, 1012, 1055, 2208
PluTI	GGCGCC	1 cut(s)	1739
PpsI	GAGTC	7 cut(s)	61, 424, 715, 937, 1012, 1055, 2208
Psp6I	CCWGG	6 cut(s)	328, 503, 838, 1909, 1927, 2339
PspFI	CCCAGC	1 cut(s)	1984
PspGI	CCWGG	6 cut(s)	328, 503, 838, 1909, 1927, 2339
PspN4I	GGNNCC	5 cut(s)	327, 678, 1286, 1737, 1827
PspOMI	GGGCCC	1 cut(s)	1284
PspPI	GGNCC	5 cut(s)	142, 180, 284, 1284, 1285
PstI	CTGCAG	1 cut(s)	1180
PstNI	CAGNNNCTG	1 cut(s)	1175
PsuI	RGATCY	3 cut(s)	1653, 2003, 2206
RruI	TCGCGA	2 cut(s)	30, 2112
RsaI	GTAC	3 cut(s)	603, 927, 1730
RsaNI	GTAC	3 cut(s)	602, 926, 1729
RseI	CAYNNNNRTG	1 cut(s)	1460
SacII	CCGCGG	1 cut(s)	74
SapI	GCTCTTC	2 cut(s)	540, 2211
Sau3AI	GATC	9 cut(s)	111, 215, 733, 908, 995, 1486, 1653, 2003, 2206
Sau96I	GGNCC	5 cut(s)	142, 180, 284, 1284, 1285
SchI	GAGTC	7 cut(s)	62, 424, 716, 937, 1013, 1055, 2209
ScrFI	CCNGG	7 cut(s)	330, 376, 505, 840, 1911, 1929, 2341
SduI	GDGCHC	2 cut(s)	1265, 1288
SexAI	ACCWGGT	1 cut(s)	328
SfaNI	GCATC	4 cut(s)	352, 1009, 1963, 2074
SfcI	CTRYAG	1 cut(s)	1176
SfoI	GGCGCC	1 cut(s)	1737
Sfr303I	CCGCGG	1 cut(s)	74
SfuI	TTCGAA	2 cut(s)	63, 1027
SgrBI	CCGCGG	1 cut(s)	74
SinI	GGWCC	1 cut(s)	180
SmiMI	CAYNNNNRTG	1 cut(s)	1460
SmlI	CTYRAG	2 cut(s)	962, 2169
SmoI	CTYRAG	2 cut(s)	962, 2169
SpeI	ACTAGT	1 cut(s)	1133
SsiI	CCGC	9 cut(s)	5, 71, 73, 109, 145, 252, 282, 351, 1829
SspDI	GGCGCC	1 cut(s)	1735
SspI	AATATT	1 cut(s)	1766
StyD4I	CCNGG	7 cut(s)	328, 374, 503, 838, 1909, 1927, 2339
StyI	CCWWGG	5 cut(s)	93, 183, 309, 435, 2211
TaaI	ACNGT	3 cut(s)	523, 688, 1701
TaiI	ACGT	1 cut(s)	2153
TaqI	TCGA	6 cut(s)	63, 224, 1027, 1236, 1416, 1779
TauI	GCSGC	4 cut(s)	8, 73, 147, 1831
TfiI	GAWTC	7 cut(s)	124, 168, 236, 371, 1154, 2095, 2324
TscAI	CASTG	5 cut(s)	201, 253, 528, 558, 918
TseFI	GTSAC	2 cut(s)	1658, 1788
TseI	GCWGC	6 cut(s)	243, 766, 821, 1178, 1516, 2087
Tsp45I	GTSAC	2 cut(s)	1658, 1788
TspGWI	ACGGA	3 cut(s)	1347, 2200, 2239
TspRI	CASTG	5 cut(s)	201, 253, 528, 558, 918
Van91I	CCANNNNNTGG	2 cut(s)	1561, 2401
Vha464I	CTTAAG	1 cut(s)	962
VpaK11BI	GGWCC	1 cut(s)	180
XagI	CCTNNNNNAGG	1 cut(s)	903
XapI	RAATTY	6 cut(s)	855, 873, 1850, 1869, 2073, 2293
XceI	RCATGY	2 cut(s)	1207, 2168
XcmI	CCANNNNNNNNNTGG	1 cut(s)	100
XmiI	GTMKAC	1 cut(s)	1703
XmnI	GAANNNNTTC	3 cut(s)	832, 1353, 2138

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.