MD13G1116600.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0010877
Physical Location & Seq
Forward (+)
8528811 .. 8533455
4645 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1116600.v1.1.491

Sequence Viewer

Length: 2148 bp
ATGGAACCTTCCAGTTTCTACGCCCTCCTCGTACTCCTCCTCCTCGTCGACACCACACTCTCACTCAACTCCGACGGCCTCTCCCTCCTCTCTCTAAAATCCGCCGTTGACCAGCCCGCCGACGGCTCCGCCTTCTCCGACTGGCTCGACACCGACCCCACCCCATGCCGCTGGACCGGCATTTCCTGCATGAACATCTCCGGCTTCCCTGACCCCCGCGTCGTCGGAATCGCCCTATCCGGGAAGAATCTCCGCGGCTACATTCCCTCCGAGCTCAGCAACCTAGTAAACCTCCGCCGCCTCAACCTCCACGACAACAACTTCCACGGCTCAATCCCTTCCCAACTCTTCAACGCCACCTCACTCCTCCACAGCATCTTCCTCTACGGCAATAACCTCTCCGGCCCGCTTCCACCGTCGATCTGCAACCCCCCGCGTCTCCAGAACCTCGACCTCTCCAACAACTCCCTCTCCGGTCCGCTCCAGAAAGACTACTTAGAGAACTGCAAGCAATTGCAGCGGCTGATCCTCGCGAGAAACAGATTCGACGGAGAAATTCCGGCGGGAATTTGGTCCGGCATGGACAATCTAAACCAATTGGATCTCTCCTCAAACGAGTTAACCGGATCGGTGCCGGATGATTTCCTGGAGTTGAAGTCTCTGTCCGGCGCGCTCAACCTATCGTACAATCACTTATCGGGAAAGATTCCGAAATCCCTCGGCGATTTACCCGTGACGGTGAGCTTGGATCTCCGAAACAATAATTTCAGCGGCGAGATTCCTCAAACGGGGTCGTTTTCGAACCAAGGCCCCACCGCGTTTCTGGACAACCCTCTGTTATGCGGGTTTCCGCTTCAGAAAACTTGTAAAACCCCGGAACAGAGCTCCGCGGATAGTCCGAATTCGGGATCAGGATCGGAAAATAGTCCGGTAAAGGGTCTGAGTCCGGGTCTGATTATCCTAATCTCGGTGGCGGACGCCGCCGTAGTGGCGTTTATCGGATTAGTCGTCGTTTACATTTACTGGAAAAAGAAAGGCGACGAGAACGGATGCAGTTGTACCGGAAAGAGCAAATTTGGCGGCAACGAGAAGCGCCACCTCTGTAATCTCTGTTCTTGCGCTTGCATCAATGGCGGGTTCGGGAACCAGGATTCGGATTCGGGCGACCCGGAAAAGGCAGAGAGGGGAAAAGGGGAGGGGGATCTCGTCGCGATCGACAAAGGGTTCACATTCGAGCTGGACGAGCTGTTGAGGGCGTCGGCGTACGTGCTGGGGAAGAGCGGACTGGGGATCGTGTACAAAGTGGTGCTCGGGAATGGAATCCCGGTGGCGGTGAGGAGGTTGGGGGAGGGCGGGGATCAGAGGTATAAGGAGTTCGCGGCGGAGATTCAGGCGATCGGGCGGGTTAAGCATCCGAATGTGGTGAAGCTGAGGGCGTACTATTGGGCGCCCGACGAGAAGCTTCTCATCAGCGACTTCATTTCTAATGGCAACTTGGCCACTGCACTTCGCGGGAGAATCGGTCAGTCATCATCAAGCCCCTCATGGACAACGAGGCTAAAGATCGCCAAGGGAACAGCCCGAGGTCTCGCCTACCTGCACGAATGCAGCCCCAGAAAGTTTGTCCACGGTGACATCAAACCCTCCAACATTCTCCTCGACAACGAATATCAAGCCTACATTTCCGATTTCGGACTCAACAGGCTGATCACAATCACAGGCAACAACCCCTCCTCCGGTGGCCTCATGGGCGGGGCTCTCCCTTACATGAAACCAGTCCAAACAGAGCGATCAAACAGCTACCGTGCCCCGGAGGCCCGTGTGACGGGCAGCAAACCCACCCAAAAATGGGACGTGTACTCGTTCGGTGTTGTGTTGCTTGAATTGCTCACAGGGAAGTCCCCGGAGCTCTCTCCGACCACGTCAACTTCGGCCGAAGTCTCAGACCTAGTGAGCTCTGTGAGGAAAGGGTTTGAGGATGAAAACCCTTTGTCGGATATGGTAGACCATGTGTTGATGCAAGAAGTGCATGCCAAAAAGGAGGTGCTTGCAGCTTTCCATGTAGCTCTTGCATGCACTGAGCTGGACCCTGAGGTTAGACCAAGGATGAAAACCGTCTCTGAAAATCTTGAAAGGGTTGGAACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

716

Amino Acids

77.18

Weight (kDa)

5.97

Isoelectric Point (pI)

33.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 22 - 63 3.3e-10 Leucine rich repeat N-terminal domain
LRR_8 PF13855 171 - 221 7e-07 Leucine rich repeat
Pkinase PF00069 423 - 659 4.7e-32 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 424 - 710 5.7e-30 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010877)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67510
fragaria_vesca FvH4_4g24010 FvH4_4g24010 FvH4_4g24010
malus_domestica MD13G1116600.v1.1 MD16G1117000.v1.1
prunus_persica Prupe.1G229200_v2.0.a1
pyrus_communis pycom13g10120 pycom16g10010
rosa_chinensis RchiOBHm_Chr4g0430671
rosa_laevigata RLG00000006957
rosa_multiflora Rmu_sc0000131.1_g000012 Rmu_sc0023719.1_g000001
rosa_roxburghii Rroxscaffold_5G00372410
rosa_rugosa Rorug04G0245100
rosa_samantha Rh4AG301200 Rh4BG308300 Rh4CG325500 Rh4DG305900
rosa_wichuraiana Rw4G026150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 218
Acc36I ACCTGC 1 cut(s) 1605
AccB1I GGYRCC 2 cut(s) 631, 1447
AccBSI CCGCTC 2 cut(s) 481, 1281
AccI GTMKAC 2 cut(s) 48, 2004
AclWI GGATC 9 cut(s) 520, 609, 634, 756, 916, 922, 1209, 1298, 1365
AcoI YGGCCR 2 cut(s) 1497, 1932
AcsI RAATTY 4 cut(s) 555, 567, 901, 1073
AcuI CTGAAG 1 cut(s) 839
AcyI GRCGYC 3 cut(s) 978, 1256, 1448
AfaI GTAC 7 cut(s) 33, 686, 1060, 1265, 1298, 1439, 1859
AflIII ACRYGT 1 cut(s) 1854
AgsI TTSAA 4 cut(s) 352, 655, 1883, 2132
AjiI CACGTC 2 cut(s) 1855, 1923
AjnI CCWGG 2 cut(s) 645, 1146
Alw21I GWGCWC 5 cut(s) 276, 887, 1311, 1911, 1958
Alw26I GTCTC 5 cut(s) 443, 663, 1592, 1945, 2122
AlwI GGATC 9 cut(s) 520, 609, 634, 756, 916, 922, 1209, 1298, 1365
AlwNI CAGNNNCTG 1 cut(s) 523
Ama87I CYCGRG 2 cut(s) 1310, 1581
AoxI GGCC 7 cut(s) 76, 403, 808, 1497, 1741, 1815, 1932
ApeKI GCWGC 4 cut(s) 517, 1608, 1830, 2051
ApoI RAATTY 4 cut(s) 555, 567, 901, 1073
AspLEI GCGC 5 cut(s) 671, 673, 1095, 1121, 1450
AspS9I GGNCC 7 cut(s) 174, 404, 476, 573, 809, 1816, 2086
AsuC2I CCSGG 7 cut(s) 241, 875, 948, 1169, 1325, 1811, 1904
AsuHPI GGTGA 4 cut(s) 751, 1345, 1435, 1643
AsuII TTCGAA 1 cut(s) 800
AvaI CYCGRG 2 cut(s) 1310, 1581
AvaII GGWCC 4 cut(s) 174, 476, 573, 2086
AxyI CCTNAGG 1 cut(s) 2091
BaeGI GKGCMC 1 cut(s) 1810
BalI TGGCCA 1 cut(s) 1499
BanI GGYRCC 2 cut(s) 631, 1447
BanII GRGCYC 5 cut(s) 276, 887, 1759, 1911, 1958
Bbv12I GWGCWC 5 cut(s) 276, 887, 1311, 1911, 1958
BbvCI CCTCAGC 1 cut(s) 1430
BbvI GCAGC 4 cut(s) 529, 1620, 1842, 2063
BceAI ACGGC 6 cut(s) 89, 91, 139, 343, 403, 968
BciT130I CCWGG 2 cut(s) 647, 1148
BclI TGATCA 1 cut(s) 1707
BcnI CCSGG 7 cut(s) 241, 875, 948, 1169, 1325, 1811, 1904
BcoDI GTCTC 5 cut(s) 443, 663, 1592, 1945, 2122
BfaI CTAG 2 cut(s) 284, 1949
BfoI RGCGCY 2 cut(s) 1096, 1451
BfuAI ACCTGC 1 cut(s) 1605
BglI GCCNNNNNGGC 3 cut(s) 989, 1749, 1814
BlpI GCTNAGC 1 cut(s) 275
Bme1390I CCNGG 9 cut(s) 241, 647, 875, 948, 1148, 1169, 1325, 1811, 1904
Bme18I GGWCC 4 cut(s) 174, 476, 573, 2086
BmeT110I CYCGRG 2 cut(s) 1310, 1581
BmgBI CACGTC 2 cut(s) 1855, 1923
BmgT120I GGNCC 7 cut(s) 174, 404, 476, 573, 809, 1816, 2086
BmiI GGNNCC 7 cut(s) 6, 127, 633, 811, 1145, 1449, 2088
BmrFI CCNGG 9 cut(s) 241, 647, 875, 948, 1148, 1169, 1325, 1811, 1904
BmrI ACTGGG 1 cut(s) 1295
BmsI GCATC 5 cut(s) 384, 1040, 1134, 1420, 2007
BmuI ACTGGG 1 cut(s) 1295
BpmI CTGGAG 3 cut(s) 425, 467, 668
Bpu10I CCTNAGC 1 cut(s) 1430
Bpu1102I GCTNAGC 1 cut(s) 275
Bpu14I TTCGAA 1 cut(s) 800
BpuMI CCSGG 7 cut(s) 241, 875, 948, 1169, 1325, 1811, 1904
BsaAI YACGTR 1 cut(s) 1267
BsaBI GATNNNNATC 2 cut(s) 913, 1712
BsaHI GRCGYC 3 cut(s) 978, 1256, 1448
BsaI GGTCTC 1 cut(s) 1592
BsaWI WCCGGW 5 cut(s) 473, 623, 928, 1061, 1736
Bse118I RCCGGY 1 cut(s) 176
Bse1I ACTGG 5 cut(s) 12, 146, 1028, 1290, 1775
Bse21I CCTNAGG 1 cut(s) 2091
Bse8I GATNNNNATC 2 cut(s) 913, 1712
BseBI CCWGG 2 cut(s) 647, 1148
BseGI GGATG 5 cut(s) 643, 1055, 1411, 1984, 2112
BseJI GATNNNNATC 2 cut(s) 913, 1712
BseMII CTCAG 6 cut(s) 289, 932, 1421, 1956, 2070, 2082
BseNI ACTGG 5 cut(s) 12, 146, 1028, 1290, 1775
BsePI GCGCGC 1 cut(s) 669
BseSI GKGCMC 1 cut(s) 1810
BseX3I CGGCCG 1 cut(s) 1932
BseXI GCAGC 4 cut(s) 529, 1620, 1842, 2063
BseYI CCCAGC 1 cut(s) 1270
BsgI GTGCAG 2 cut(s) 1488, 1583
Bsh1285I CGRYCG 3 cut(s) 1215, 1398, 1935
BshFI GGCC 7 cut(s) 78, 405, 810, 1499, 1743, 1817, 1934
BshNI GGYRCC 2 cut(s) 631, 1447
BsiEI CGRYCG 3 cut(s) 1215, 1398, 1935
BsiHKAI GWGCWC 5 cut(s) 276, 887, 1311, 1911, 1958
BsiHKCI CYCGRG 2 cut(s) 1310, 1581
BsiWI CGTACG 1 cut(s) 1263
BslFI GGGAC 2 cut(s) 1865, 1885
BsmAI GTCTC 5 cut(s) 443, 663, 1592, 1945, 2122
BsmBI CGTCTC 2 cut(s) 443, 2122
BsmFI GGGAC 2 cut(s) 1865, 1885
BsmI GAATGC 1 cut(s) 1610
BsnI GGCC 7 cut(s) 78, 405, 810, 1499, 1743, 1817, 1934
Bso31I GGTCTC 1 cut(s) 1592
BsoBI CYCGRG 2 cut(s) 1310, 1581
Bsp119I TTCGAA 1 cut(s) 800
Bsp1286I GDGCHC 7 cut(s) 276, 887, 1311, 1759, 1810, 1911, 1958
Bsp1407I TGTACA 1 cut(s) 1296
Bsp1720I GCTNAGC 1 cut(s) 275
Bsp68I TCGCGA 2 cut(s) 533, 1211
BspANI GGCC 7 cut(s) 78, 405, 810, 1499, 1743, 1817, 1934
BspCNI CTCAG 6 cut(s) 288, 933, 1422, 1955, 2071, 2083
BspLI GGNNCC 7 cut(s) 6, 127, 633, 811, 1145, 1449, 2088
BspMI ACCTGC 1 cut(s) 1605
BspPI GGATC 9 cut(s) 520, 609, 634, 756, 916, 922, 1209, 1298, 1365
BspQI GCTCTTC 1 cut(s) 1271
BspT104I TTCGAA 1 cut(s) 800
BspT107I GGYRCC 2 cut(s) 631, 1447
BspTNI GGTCTC 1 cut(s) 1592
BsrBI CCGCTC 2 cut(s) 481, 1281
BsrFI RCCGGY 1 cut(s) 176
BsrGI TGTACA 1 cut(s) 1296
BsrI ACTGG 5 cut(s) 12, 146, 1028, 1290, 1775
BssAI RCCGGY 1 cut(s) 176
BssHII GCGCGC 1 cut(s) 669
BssNI GRCGYC 3 cut(s) 978, 1256, 1448
BssT1I CCWWGG 3 cut(s) 805, 1569, 2102
Bst2UI CCWGG 2 cut(s) 647, 1148
Bst4CI ACNGT 5 cut(s) 417, 739, 1631, 1805, 2116
Bst6I CTCTTC 2 cut(s) 353, 1271
BstACI GRCGYC 3 cut(s) 978, 1256, 1448
BstAPI GCANNNNNTGC 2 cut(s) 186, 2026
BstAUI TGTACA 1 cut(s) 1296
BstBAI YACGTR 1 cut(s) 1267
BstBI TTCGAA 1 cut(s) 800
BstC8I GCNNGC 8 cut(s) 117, 407, 509, 671, 1123, 2031, 2049, 2074
BstDEI CTNAG 7 cut(s) 275, 496, 941, 1430, 1942, 2079, 2091
BstDSI CCRYGG 4 cut(s) 253, 325, 888, 1627
BstF5I GGATG 5 cut(s) 643, 1055, 1411, 1984, 2112
BstH2I RGCGCY 2 cut(s) 1096, 1451
BstHHI GCGC 5 cut(s) 671, 673, 1095, 1121, 1450
BstMAI GTCTC 5 cut(s) 443, 663, 1592, 1945, 2122
BstMCI CGRYCG 3 cut(s) 1215, 1398, 1935
BstNI CCWGG 2 cut(s) 647, 1148
BstNSI RCATGY 2 cut(s) 2033, 2076
BstSCI CCNGG 9 cut(s) 239, 645, 873, 946, 1146, 1167, 1323, 1809, 1902
BstSLI GKGCMC 1 cut(s) 1810
BstV1I GCAGC 4 cut(s) 529, 1620, 1842, 2063
BstX2I RGATCY 3 cut(s) 601, 748, 1201
BstXI CCANNNNNNTGG 1 cut(s) 171
BstYI RGATCY 3 cut(s) 601, 748, 1201
BstZI CGGCCG 1 cut(s) 1932
Bsu36I CCTNAGG 1 cut(s) 2091
BsuRI GGCC 7 cut(s) 78, 405, 810, 1499, 1743, 1817, 1934
BtgI CCRYGG 4 cut(s) 253, 325, 888, 1627
BtrI CACGTC 2 cut(s) 1855, 1923
BtsCI GGATG 5 cut(s) 643, 1055, 1411, 1984, 2112
BtsI GCAGTG 1 cut(s) 1500
BtsIMutI CAGTG 2 cut(s) 1500, 2076
BtuMI TCGCGA 2 cut(s) 533, 1211
BveI ACCTGC 1 cut(s) 1605
Cac8I GCNNGC 8 cut(s) 117, 407, 509, 671, 1123, 2031, 2049, 2074
CaiI CAGNNNCTG 1 cut(s) 523
CfoI GCGC 5 cut(s) 671, 673, 1095, 1121, 1450
Cfr10I RCCGGY 1 cut(s) 176
Cfr13I GGNCC 7 cut(s) 174, 404, 476, 573, 809, 1816, 2086
Cfr42I CCGCGG 2 cut(s) 256, 891
CpoI CGGWCCG 1 cut(s) 476
CseI GACGC 4 cut(s) 208, 425, 986, 1245
Csp6I GTAC 7 cut(s) 32, 685, 1059, 1264, 1297, 1438, 1858
CspI CGGWCCG 1 cut(s) 476
CviQI GTAC 7 cut(s) 32, 685, 1059, 1264, 1297, 1438, 1858
DdeI CTNAG 7 cut(s) 275, 496, 941, 1430, 1942, 2079, 2091
DinI GGCGCC 1 cut(s) 1449
DrdI GACNNNNNNGTC 1 cut(s) 218
DseDI GACNNNNNNGTC 1 cut(s) 218
EaeI YGGCCR 2 cut(s) 1497, 1932
EagI CGGCCG 1 cut(s) 1932
Eam1104I CTCTTC 2 cut(s) 353, 1271
EarI CTCTTC 2 cut(s) 353, 1271
EciI GGCGGA 5 cut(s) 91, 118, 284, 989, 1397
Ecl136II GAGCTC 4 cut(s) 274, 885, 1909, 1956
EclXI CGGCCG 1 cut(s) 1932
Eco130I CCWWGG 3 cut(s) 805, 1569, 2102
Eco24I GRGCYC 5 cut(s) 276, 887, 1759, 1911, 1958
Eco31I GGTCTC 1 cut(s) 1592
Eco47I GGWCC 4 cut(s) 174, 476, 573, 2086
Eco52I CGGCCG 1 cut(s) 1932
Eco53kI GAGCTC 4 cut(s) 274, 885, 1909, 1956
Eco57I CTGAAG 1 cut(s) 839
Eco81I CCTNAGG 1 cut(s) 2091
Eco88I CYCGRG 2 cut(s) 1310, 1581
EcoICRI GAGCTC 4 cut(s) 274, 885, 1909, 1956
EcoO109I RGGNCCY 1 cut(s) 809
EcoRI GAATTC 1 cut(s) 901
EcoRII CCWGG 2 cut(s) 645, 1146
EcoT14I CCWWGG 3 cut(s) 805, 1569, 2102
EcoT38I GRGCYC 5 cut(s) 276, 887, 1759, 1911, 1958
EgeI GGCGCC 1 cut(s) 1449
EheI GGCGCC 1 cut(s) 1449
ErhI CCWWGG 3 cut(s) 805, 1569, 2102
Esp3I CGTCTC 2 cut(s) 443, 2122
FaqI GGGAC 2 cut(s) 1865, 1885
FbaI TGATCA 1 cut(s) 1707
FblI GTMKAC 2 cut(s) 48, 2004
FokI GGATG 5 cut(s) 650, 1062, 1398, 1991, 2119
FriOI GRGCYC 5 cut(s) 276, 887, 1759, 1911, 1958
FspBI CTAG 2 cut(s) 284, 1949
GlaI GCGC 5 cut(s) 670, 672, 1094, 1120, 1449
GsaI CCCAGC 1 cut(s) 1274
GsuI CTGGAG 3 cut(s) 425, 467, 668
HaeII RGCGCY 2 cut(s) 1096, 1451
HaeIII GGCC 7 cut(s) 78, 405, 810, 1499, 1743, 1817, 1934
HgaI GACGC 4 cut(s) 208, 425, 986, 1245
HhaI GCGC 5 cut(s) 671, 673, 1095, 1121, 1450
Hin1I GRCGYC 3 cut(s) 978, 1256, 1448
Hin6I GCGC 5 cut(s) 669, 671, 1093, 1119, 1448
HinP1I GCGC 5 cut(s) 669, 671, 1093, 1119, 1448
HincII GTYRAC 4 cut(s) 49, 109, 621, 1926
HindII GTYRAC 4 cut(s) 49, 109, 621, 1926
HindIII AAGCTT 1 cut(s) 1460
HpaI GTTAAC 1 cut(s) 621
HphI GGTGA 4 cut(s) 751, 1345, 1435, 1643
HpyAV CCTTC 3 cut(s) 18, 142, 348
HpyCH4III ACNGT 5 cut(s) 417, 739, 1631, 1805, 2116
HpyCH4IV ACGT 3 cut(s) 1266, 1854, 1922
HpyF3I CTNAG 7 cut(s) 275, 496, 941, 1430, 1942, 2079, 2091
HpySE526I ACGT 3 cut(s) 1266, 1854, 1922
Hsp92I GRCGYC 3 cut(s) 978, 1256, 1448
HspAI GCGC 5 cut(s) 669, 671, 1093, 1119, 1448
KasI GGCGCC 1 cut(s) 1447
Ksp22I TGATCA 1 cut(s) 1707
KspAI GTTAAC 1 cut(s) 621
KspI CCGCGG 2 cut(s) 256, 891
LguI GCTCTTC 1 cut(s) 1271
LmnI GCTCC 4 cut(s) 131, 486, 890, 1906
Lsp1109I GCAGC 4 cut(s) 529, 1620, 1842, 2063
LweI GCATC 5 cut(s) 384, 1040, 1134, 1420, 2007
MaeI CTAG 2 cut(s) 284, 1949
MaeII ACGT 3 cut(s) 1266, 1854, 1922
MaeIII GTNAC 3 cut(s) 733, 1631, 1822
MbiI CCGCTC 2 cut(s) 481, 1281
MboII GAAGA 4 cut(s) 256, 340, 370, 1288
MfeI CAATTG 2 cut(s) 512, 596
MflI RGATCY 3 cut(s) 601, 748, 1201
MhlI GDGCHC 7 cut(s) 276, 887, 1311, 1759, 1810, 1911, 1958
MlsI TGGCCA 1 cut(s) 1499
MluCI AATT 8 cut(s) 512, 555, 567, 596, 763, 901, 1073, 1883
MluNI TGGCCA 1 cut(s) 1499
Mly113I GGCGCC 1 cut(s) 1448
MlyI GAGTC 2 cut(s) 952, 1689
MmeI TCCRAC 8 cut(s) 96, 162, 205, 483, 1671, 1940, 1974, 2119
Mox20I TGGCCA 1 cut(s) 1499
MscI TGGCCA 1 cut(s) 1499
MseI TTAA 2 cut(s) 620, 1407
MslI CAYNNNNRTG 1 cut(s) 1416
Msp20I TGGCCA 1 cut(s) 1499
MspA1I CMGCKG 5 cut(s) 171, 255, 520, 771, 890
MspR9I CCNGG 9 cut(s) 241, 647, 875, 948, 1148, 1169, 1325, 1811, 1904
MunI CAATTG 2 cut(s) 512, 596
Mva1269I GAATGC 1 cut(s) 1610
MvaI CCWGG 2 cut(s) 647, 1148
NarI GGCGCC 1 cut(s) 1448
NciI CCSGG 7 cut(s) 241, 875, 948, 1169, 1325, 1811, 1904
NlaIV GGNNCC 7 cut(s) 6, 127, 633, 811, 1145, 1449, 2088
NmeAIII GCCGAG 1 cut(s) 699
NmuCI GTSAC 3 cut(s) 733, 1631, 1822
NruI TCGCGA 2 cut(s) 533, 1211
NspI RCATGY 2 cut(s) 2033, 2076
NspV TTCGAA 1 cut(s) 800
PaeI GCATGC 2 cut(s) 2033, 2076
PauI GCGCGC 1 cut(s) 669
PciSI GCTCTTC 1 cut(s) 1271
PcsI WCGNNNNNNNCGW 4 cut(s) 27, 1005, 1212, 1239
PctI GAATGC 1 cut(s) 1610
Pfl23II CGTACG 1 cut(s) 1263
PflFI GACNNNGTC 1 cut(s) 1921
PfoI TCCNGGA 2 cut(s) 239, 645
Ple19I CGATCG 2 cut(s) 1215, 1398
PleI GAGTC 2 cut(s) 951, 1689
PluTI GGCGCC 1 cut(s) 1451
PpsI GAGTC 2 cut(s) 951, 1689
Ppu21I YACGTR 1 cut(s) 1267
Psp124BI GAGCTC 4 cut(s) 276, 887, 1911, 1958
Psp6I CCWGG 2 cut(s) 645, 1146
PspFI CCCAGC 1 cut(s) 1270
PspGI CCWGG 2 cut(s) 645, 1146
PspLI CGTACG 1 cut(s) 1263
PspN4I GGNNCC 7 cut(s) 6, 127, 633, 811, 1145, 1449, 2088
PspPI GGNCC 7 cut(s) 174, 404, 476, 573, 809, 1816, 2086
PsrI GAACNNNNNNTAC 4 cut(s) 1096, 1128, 1358, 1390
PstNI CAGNNNCTG 1 cut(s) 523
PsuI RGATCY 3 cut(s) 601, 748, 1201
PsyI GACNNNGTC 1 cut(s) 1921
PteI GCGCGC 1 cut(s) 669
PvuI CGATCG 2 cut(s) 1215, 1398
RruI TCGCGA 2 cut(s) 533, 1211
RsaI GTAC 7 cut(s) 33, 686, 1060, 1265, 1298, 1439, 1859
RsaNI GTAC 7 cut(s) 32, 685, 1059, 1264, 1297, 1438, 1858
RseI CAYNNNNRTG 1 cut(s) 1416
Rsr2I CGGWCCG 1 cut(s) 476
RsrII CGGWCCG 1 cut(s) 476
SacI GAGCTC 4 cut(s) 276, 887, 1911, 1958
SacII CCGCGG 2 cut(s) 256, 891
SalI GTCGAC 1 cut(s) 47
SapI GCTCTTC 1 cut(s) 1271
SaqAI TTAA 2 cut(s) 620, 1407
Sau96I GGNCC 7 cut(s) 174, 404, 476, 573, 809, 1816, 2086
SchI GAGTC 2 cut(s) 952, 1689
ScrFI CCNGG 9 cut(s) 241, 647, 875, 948, 1148, 1169, 1325, 1811, 1904
SduI GDGCHC 7 cut(s) 276, 887, 1311, 1759, 1810, 1911, 1958
SfaNI GCATC 5 cut(s) 384, 1040, 1134, 1420, 2007
SfoI GGCGCC 1 cut(s) 1449
Sfr303I CCGCGG 2 cut(s) 256, 891
SfuI TTCGAA 1 cut(s) 800
SgrBI CCGCGG 2 cut(s) 256, 891
SinI GGWCC 4 cut(s) 174, 476, 573, 2086
SmiMI CAYNNNNRTG 1 cut(s) 1416
SphI GCATGC 2 cut(s) 2033, 2076
Sse9I AATT 8 cut(s) 512, 555, 567, 596, 763, 901, 1073, 1883
SspDI GGCGCC 1 cut(s) 1447
SspMI CTAG 2 cut(s) 284, 1949
SstI GAGCTC 4 cut(s) 276, 887, 1911, 1958
StyD4I CCNGG 9 cut(s) 239, 645, 873, 946, 1146, 1167, 1323, 1809, 1902
StyI CCWWGG 3 cut(s) 805, 1569, 2102
TaaI ACNGT 5 cut(s) 417, 739, 1631, 1805, 2116
TaiI ACGT 3 cut(s) 1269, 1857, 1925
TaqI TCGA 9 cut(s) 48, 147, 419, 450, 546, 800, 1215, 1233, 1659
TaqII GACCGA 1 cut(s) 1511
TasI AATT 8 cut(s) 512, 555, 567, 596, 763, 901, 1073, 1883
TatI WGTACW 2 cut(s) 1296, 1857
TauI GCSGC 8 cut(s) 171, 258, 300, 523, 774, 983, 1083, 1382
Tru1I TTAA 2 cut(s) 620, 1407
Tru9I TTAA 2 cut(s) 620, 1407
TscAI CASTG 2 cut(s) 1507, 2083
TseFI GTSAC 3 cut(s) 733, 1631, 1822
TseI GCWGC 4 cut(s) 517, 1608, 1830, 2051
Tsp45I GTSAC 3 cut(s) 733, 1631, 1822
TspDTI ATGAA 5 cut(s) 206, 1468, 1784, 1995, 2123
TspGWI ACGGA 2 cut(s) 564, 1062
TspRI CASTG 2 cut(s) 1507, 2083
Tth111I GACNNNGTC 1 cut(s) 1921
VpaK11BI GGWCC 4 cut(s) 174, 476, 573, 2086
XapI RAATTY 4 cut(s) 555, 567, 901, 1073
XceI RCATGY 2 cut(s) 2033, 2076
XcmI CCANNNNNNNNNTGG 1 cut(s) 820
XmiI GTMKAC 2 cut(s) 48, 2004
XspI CTAG 2 cut(s) 284, 1949
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.