Rh4DG305900
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0010877
Physical Location & Seq
Reverse (-)
52773455 .. 52778498
5044 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG305900.1

Sequence Viewer

Length: 2127 bp
ATGAAGGATTCCACTATTTACTCACTCTTCTTCTTCTTCTTCTGTTTTGGCGACTTCGCTGAGTCTTTGACTCCCGACGGACTCTCCCTCCTCTCCCTCAAATCCGCCGTCGACCAGTCCACCGCCGGCTCCGCCTTCTCCGACTGGGCCGACTCCGACGCCTCGCCCTGCCGATGGACCGGCATTTCCTGCATGAACATCACCGGATTCCCCGACCCCCGCGTCGTCGGGATCGCGCTCTCCGGCAAGAATCTCCGGGGCTACATCCCCTCCGAGCTCGGAAACTTAGTCTATCTGCGGCGGCTCAACCTCCACAGCAACAACTTCTACGGCGCCATACCGACTCAGCTCTTCAATGCCACGTCACTCCACAGCCTCTTCCTCTACGGTAACAACCTCTCCGGTCAGCTCCCGCCCTCGATCTGTAACCTCCCGCGCCTCCAGAACCTCGACCTCTCCAACAACTCCCTCTCCGGTTCGTTAGACAAGGGCCTCAACAACTGTAAGCAATTGCAGAGACTGATACTCGCCGGAAACCATTTCACTGGCGAAATCCCGGCCGGAATTTGGTCCGGTATGGAAAACCTCCTCCAATTGGACCTCTCCGACAACGGTTTCACCGGATCGGTGCCGGAAGATTTCGGCGACCTGAAGTCTCTCTCCGGCACGCTCAACCTCTCGTTCAATCACCTATCCGGCGGGATTCCGAAATCGCTGGGAGACTTACCGGTGACGGTGAGCTTCGATCTCCGCAACAATAATTTCAGCGGCGAAATTCCCCAAACGGGGTCGTTTTCGAACCAGGGCCCGACCGCGTTTCTCAACAACCCTCTCTTATGCGGGTTTCCGCTCCAGAAATCTTGCAAAGACCAGGCCCCGAGTTCCCCGGGTAACCCGAATTCCTCGCCCGGATCCGATAACAACCCGAAAAAGGGGTTGAGTCCTGGTCTGATCATCCTCATCTCCGTAGCCGACGCCGCCGCAGTCGCATTCATCGGTTTGGTCGTAGTTTACATTTACTGGAAAAAGAAGGACAATGCCAACGGCTGTTCGTGTACCGGAAAGACAAAATTCGGCGCCAACACCGAGAAAAGCCGACTCTGTTGTTCTTGCATCAACGGCGGGTTCGGGAACTCGGACTCCGAACTCGACCCGCCCGAGAGAGGAAGAGGCGAAGGAGAGCTTGTGGCAATCGATAAGGGGTTCACATTTGAACTCGACGAGCTGCTGAGAGCGTCGGCTTATGTTCTGGGGAAGAGTGGGTTGGGGATTGTGTACAAGGTGGTGCTGGGGAATGGGATTCCGGTGGCGGTGAGGAGGTTGGGGGAGGGCGGCGATCAGAGGTACAAGGAGTTCGCGGCCGAGGTTCAGGCGATCGGTAGAGTGAAGCATCCCAACGTGGTGAGGCTGAGGGCGTACTATTGGGCCCCCGACGAGAAGCTCTTGATCAGTGACTTTATTTCGCATGGGAACTTGGCCTCTGCGATTCGCGGGAGAAATGGTCAGCCTATAAGCCTCTCATGGTCAACGAGGCTGAAGATCTTAAAGGGAACAGCGCGAGGCTTGGCCTATCTCCACGAATGCAGCCCCCGAAAATTTGTCCATGGAGACATCAAACCTTCCAACATTCTACTCGACAACGAATCTCAACCCTACATTTCGGATTTCGGACTCAACAGACTGATCACCATAACTGGCAACAACCCCTCCTCGGGTGGCTTGATGGGTAGCGCTCTCCCTTACATGAAGTCTAGCCAAACGGAGCGAGCCAACAACTACAGAGCTCCGGAGGCTAGAGTCCCGGGTAATAAACCAACTCAAAAATGGGATGTTTATTCATTCGGAGTTGTCCTGCTGGAGTTGCTCACAGGAAAGTCTCCAGAGCTATCTCCAACTAGTACCTCAACTTCCCTAGAGATACCGGACTTAGTGAGGTGGGTGAGGAAAGGATTTGAGGATGAAAACCCTCTTTCGGATATGGTAGACCCTGTGTTGCTTCAAGAAGTTCATGCCAAAAAGGAAGTGCTAGCAGCTTTTCATGTAGCACTTGCTTGTACTGAGCCTGATCCTGAGGTTAGGCCGAGGATGAAAACTGTCTCCGAGAATCTCGAAAGGGTTGGATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

708

Amino Acids

76.54

Weight (kDa)

6.8

Isoelectric Point (pI)

32.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 25 - 64 8.5e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 67 - 176 5.8e-07 Leucine-rich repeat region
LRR_8 PF13855 99 - 157 2.2e-06 Leucine rich repeat
LRR_8 PF13855 170 - 231 6.2e-08 Leucine rich repeat
Pkinase PF00069 416 - 646 1.7e-31 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 417 - 703 1.3e-28 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010877)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67510
fragaria_vesca FvH4_4g24010 FvH4_4g24010 FvH4_4g24010
malus_domestica MD13G1116600.v1.1 MD16G1117000.v1.1
prunus_persica Prupe.1G229200_v2.0.a1
pyrus_communis pycom13g10120 pycom16g10010
rosa_chinensis RchiOBHm_Chr4g0430671
rosa_laevigata RLG00000006957
rosa_multiflora Rmu_sc0000131.1_g000012 Rmu_sc0023719.1_g000001
rosa_roxburghii Rroxscaffold_5G00372410
rosa_rugosa Rorug04G0245100
rosa_samantha Rh4AG301200 Rh4BG308300 Rh4CG325500 Rh4DG305900
rosa_wichuraiana Rw4G026150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 221
AccB1I GGYRCC 3 cut(s) 332, 628, 1076
AccBSI CCGCTC 1 cut(s) 850
AccI GTMKAC 2 cut(s) 111, 1983
AccII CGCG 7 cut(s) 222, 236, 436, 815, 1358, 1491, 1558
AccIII TCCGGA 1 cut(s) 1786
AclWI GGATC 5 cut(s) 239, 631, 906, 919, 2060
AcoI YGGCCR 2 cut(s) 558, 1359
AcsI RAATTY 5 cut(s) 564, 774, 898, 1070, 1595
AcuI CTGAAG 2 cut(s) 671, 1556
AcyI GRCGYC 4 cut(s) 159, 333, 975, 1077
AfaI GTAC 6 cut(s) 1057, 1277, 1346, 1418, 1900, 2056
AfeI AGCGCT 1 cut(s) 1732
AgeI ACCGGT 1 cut(s) 727
AgsI TTSAA 4 cut(s) 355, 685, 1214, 2000
AhdI GACNNNNNGTC 1 cut(s) 652
AhlI ACTAGT 1 cut(s) 1895
AjiI CACGTC 1 cut(s) 363
AjnI CCWGG 3 cut(s) 801, 870, 943
AjuI GAANNNNNNNTTGG 2 cut(s) 1247, 1279
AloI GAACNNNNNNTCC 2 cut(s) 1124, 1156
Alw21I GWGCWC 2 cut(s) 279, 1786
Alw26I GTCTC 6 cut(s) 511, 660, 714, 1602, 1881, 2101
AlwI GGATC 5 cut(s) 239, 631, 906, 919, 2060
AlwNI CAGNNNCTG 1 cut(s) 520
Ama87I CYCGRG 5 cut(s) 877, 886, 1157, 1711, 1801
Aor13HI TCCGGA 1 cut(s) 1786
Aor51HI AGCGCT 1 cut(s) 1732
ApaI GGGCCC 2 cut(s) 809, 1429
ApeKI GCWGC 3 cut(s) 1225, 1584, 2030
ApoI RAATTY 5 cut(s) 564, 774, 898, 1070, 1595
ArsI GACNNNNNNTTYG 2 cut(s) 93, 125
AsiGI ACCGGT 1 cut(s) 727
AspLEI GCGC 6 cut(s) 238, 335, 438, 1079, 1558, 1733
AsuC2I CCSGG 7 cut(s) 257, 557, 887, 888, 909, 1802, 1803
AsuHPI GGTGA 9 cut(s) 193, 610, 680, 742, 748, 1324, 1414, 1678, 1951
AsuII TTCGAA 1 cut(s) 797
AsuNHI GCTAGC 1 cut(s) 2026
AvaI CYCGRG 5 cut(s) 877, 886, 1157, 1711, 1801
AvaII GGWCC 3 cut(s) 177, 570, 598
AxyI CCTNAGG 1 cut(s) 2070
BaeGI GKGCMC 2 cut(s) 809, 1429
BamHI GGATCC 1 cut(s) 911
BanI GGYRCC 3 cut(s) 332, 628, 1076
BanII GRGCYC 4 cut(s) 279, 809, 1429, 1786
BarI GAAGNNNNNNTAC 2 cut(s) 1891, 1923
Bbv12I GWGCWC 2 cut(s) 279, 1786
BbvCI CCTCAGC 1 cut(s) 1409
BbvI GCAGC 3 cut(s) 1212, 1596, 2042
BccI CCATC 2 cut(s) 168, 1717
BceAI ACGGC 4 cut(s) 92, 346, 1060, 1135
BciT130I CCWGG 3 cut(s) 803, 872, 945
BclI TGATCA 3 cut(s) 951, 1446, 1683
BcnI CCSGG 7 cut(s) 257, 557, 887, 888, 909, 1802, 1803
BcoDI GTCTC 6 cut(s) 511, 660, 714, 1602, 1881, 2101
BcuI ACTAGT 1 cut(s) 1895
BfaI CTAG 5 cut(s) 1752, 1794, 1896, 1913, 2027
BfmI CTRYAG 1 cut(s) 1777
BfoI RGCGCY 3 cut(s) 336, 1080, 1734
BglII AGATCT 1 cut(s) 1539
Bme18I GGWCC 3 cut(s) 177, 570, 598
BmeRI GACNNNNNGTC 1 cut(s) 652
BmeT110I CYCGRG 5 cut(s) 877, 886, 1157, 1711, 1801
BmgBI CACGTC 1 cut(s) 363
BmiI GGNNCC 9 cut(s) 130, 334, 630, 807, 876, 913, 1078, 1427, 1428
BmrI ACTGGG 1 cut(s) 154
BmsI GCATC 2 cut(s) 1122, 1399
BmtI GCTAGC 1 cut(s) 2030
BmuI ACTGGG 1 cut(s) 154
BpmI CTGGAG 4 cut(s) 425, 836, 1863, 1877
Bpu10I CCTNAGC 1 cut(s) 1409
Bpu14I TTCGAA 1 cut(s) 797
BpuMI CCSGG 7 cut(s) 257, 557, 887, 888, 909, 1802, 1803
Bsa29I ATCGAT 1 cut(s) 1194
BsaHI GRCGYC 4 cut(s) 159, 333, 975, 1077
BsaJI CCNNGG 9 cut(s) 256, 802, 885, 886, 1362, 1603, 1710, 1801, 2081
Bse118I RCCGGY 3 cut(s) 125, 179, 727
Bse1I ACTGG 5 cut(s) 115, 149, 550, 1025, 1699
Bse21I CCTNAGG 1 cut(s) 2070
BseAI TCCGGA 1 cut(s) 1786
BseBI CCWGG 3 cut(s) 803, 872, 945
BseCI ATCGAT 1 cut(s) 1194
BseDI CCNNGG 9 cut(s) 256, 802, 885, 886, 1362, 1603, 1710, 1801, 2081
BseGI GGATG 6 cut(s) 264, 954, 1390, 1834, 1963, 2091
BseMII CTCAG 6 cut(s) 51, 359, 1220, 1400, 2049, 2061
BseNI ACTGG 5 cut(s) 115, 149, 550, 1025, 1699
BseRI GAGGAG 4 cut(s) 80, 578, 1330, 1699
BseSI GKGCMC 2 cut(s) 809, 1429
BseX3I CGGCCG 2 cut(s) 558, 1359
BseXI GCAGC 3 cut(s) 1212, 1596, 2042
BseYI CCCAGC 2 cut(s) 715, 1288
Bsh1236I CGCG 7 cut(s) 222, 236, 436, 815, 1358, 1491, 1558
Bsh1285I CGRYCG 4 cut(s) 561, 813, 1362, 1377
BshNI GGYRCC 3 cut(s) 332, 628, 1076
BshTI ACCGGT 1 cut(s) 727
BshVI ATCGAT 1 cut(s) 1194
BsiEI CGRYCG 4 cut(s) 561, 813, 1362, 1377
BsiHKAI GWGCWC 2 cut(s) 279, 1786
BsiHKCI CYCGRG 5 cut(s) 877, 886, 1157, 1711, 1801
BslFI GGGAC 1 cut(s) 1784
BsmAI GTCTC 6 cut(s) 511, 660, 714, 1602, 1881, 2101
BsmFI GGGAC 1 cut(s) 1784
BsmI GAATGC 2 cut(s) 989, 1586
BsoBI CYCGRG 5 cut(s) 877, 886, 1157, 1711, 1801
Bsp119I TTCGAA 1 cut(s) 797
Bsp120I GGGCCC 2 cut(s) 805, 1425
Bsp1286I GDGCHC 4 cut(s) 279, 809, 1429, 1786
Bsp13I TCCGGA 1 cut(s) 1786
Bsp1407I TGTACA 1 cut(s) 1275
Bsp19I CCATGG 1 cut(s) 1603
BspCNI CTCAG 6 cut(s) 52, 358, 1221, 1401, 2050, 2062
BspDI ATCGAT 1 cut(s) 1194
BspEI TCCGGA 1 cut(s) 1786
BspFNI CGCG 7 cut(s) 222, 236, 436, 815, 1358, 1491, 1558
BspHI TCATGA 1 cut(s) 2123
BspLI GGNNCC 9 cut(s) 130, 334, 630, 807, 876, 913, 1078, 1427, 1428
BspOI GCTAGC 1 cut(s) 2030
BspPI GGATC 5 cut(s) 239, 631, 906, 919, 2060
BspQI GCTCTTC 1 cut(s) 356
BspT104I TTCGAA 1 cut(s) 797
BspT107I GGYRCC 3 cut(s) 332, 628, 1076
BsrBI CCGCTC 1 cut(s) 850
BsrFI RCCGGY 3 cut(s) 125, 179, 727
BsrGI TGTACA 1 cut(s) 1275
BsrI ACTGG 5 cut(s) 115, 149, 550, 1025, 1699
BssAI RCCGGY 3 cut(s) 125, 179, 727
BssECI CCNNGG 9 cut(s) 256, 802, 885, 886, 1362, 1603, 1710, 1801, 2081
BssNI GRCGYC 4 cut(s) 159, 333, 975, 1077
BssT1I CCWWGG 1 cut(s) 1603
Bst2UI CCWGG 3 cut(s) 803, 872, 945
Bst4CI ACNGT 5 cut(s) 389, 503, 614, 736, 2095
Bst6I CTCTTC 5 cut(s) 32, 356, 383, 1162, 1250
BstACI GRCGYC 4 cut(s) 159, 333, 975, 1077
BstAPI GCANNNNNTGC 1 cut(s) 189
BstAUI TGTACA 1 cut(s) 1275
BstBI TTCGAA 1 cut(s) 797
BstC8I GCNNGC 4 cut(s) 127, 668, 1767, 2028
BstDEI CTNAG 8 cut(s) 60, 286, 345, 1229, 1409, 1927, 2058, 2070
BstDSI CCRYGG 1 cut(s) 1603
BstEII GGTNACC 1 cut(s) 890
BstF5I GGATG 6 cut(s) 264, 954, 1390, 1834, 1963, 2091
BstFNI CGCG 7 cut(s) 222, 236, 436, 815, 1358, 1491, 1558
BstH2I RGCGCY 3 cut(s) 336, 1080, 1734
BstHHI GCGC 6 cut(s) 238, 335, 438, 1079, 1558, 1733
BstMAI GTCTC 6 cut(s) 511, 660, 714, 1602, 1881, 2101
BstMCI CGRYCG 4 cut(s) 561, 813, 1362, 1377
BstMWI GCNNNNNNNGC 7 cut(s) 131, 189, 977, 986, 1119, 1790, 1861
BstNI CCWGG 3 cut(s) 803, 872, 945
BstPI GGTNACC 1 cut(s) 890
BstSFI CTRYAG 1 cut(s) 1777
BstSLI GKGCMC 2 cut(s) 809, 1429
BstUI CGCG 7 cut(s) 222, 236, 436, 815, 1358, 1491, 1558
BstV1I GCAGC 3 cut(s) 1212, 1596, 2042
BstX2I RGATCY 2 cut(s) 911, 1539
BstXI CCANNNNNNTGG 1 cut(s) 545
BstYI RGATCY 2 cut(s) 911, 1539
BstZI CGGCCG 2 cut(s) 558, 1359
Bsu15I ATCGAT 1 cut(s) 1194
Bsu36I CCTNAGG 1 cut(s) 2070
BsuTUI ATCGAT 1 cut(s) 1194
BtgI CCRYGG 1 cut(s) 1603
BtrI CACGTC 1 cut(s) 363
BtsCI GGATG 6 cut(s) 264, 954, 1390, 1834, 1963, 2091
BtsIMutI CAGTG 2 cut(s) 543, 1456
Cac8I GCNNGC 4 cut(s) 127, 668, 1767, 2028
CaiI CAGNNNCTG 1 cut(s) 520
CciI TCATGA 1 cut(s) 2123
CfoI GCGC 6 cut(s) 238, 335, 438, 1079, 1558, 1733
Cfr10I RCCGGY 3 cut(s) 125, 179, 727
Cfr9I CCCGGG 2 cut(s) 886, 1801
ClaI ATCGAT 1 cut(s) 1194
CseI GACGC 4 cut(s) 167, 211, 983, 1224
Csp6I GTAC 6 cut(s) 1056, 1276, 1345, 1417, 1899, 2055
CspAI ACCGGT 1 cut(s) 727
CviAII CATG 8 cut(s) 193, 1466, 1521, 1604, 1744, 2009, 2039, 2124
CviQI GTAC 6 cut(s) 1056, 1276, 1345, 1417, 1899, 2055
DdeI CTNAG 8 cut(s) 60, 286, 345, 1229, 1409, 1927, 2058, 2070
DinI GGCGCC 2 cut(s) 334, 1078
DrdI GACNNNNNNGTC 1 cut(s) 221
DriI GACNNNNNGTC 1 cut(s) 652
DseDI GACNNNNNNGTC 1 cut(s) 221
EaeI YGGCCR 2 cut(s) 558, 1359
EagI CGGCCG 2 cut(s) 558, 1359
Eam1104I CTCTTC 5 cut(s) 32, 356, 383, 1162, 1250
Eam1105I GACNNNNNGTC 1 cut(s) 652
EarI CTCTTC 5 cut(s) 32, 356, 383, 1162, 1250
EciI GGCGGA 2 cut(s) 94, 121
Ecl136II GAGCTC 2 cut(s) 277, 1784
EclXI CGGCCG 2 cut(s) 558, 1359
Eco130I CCWWGG 1 cut(s) 1603
Eco24I GRGCYC 4 cut(s) 279, 809, 1429, 1786
Eco47I GGWCC 3 cut(s) 177, 570, 598
Eco47III AGCGCT 1 cut(s) 1732
Eco52I CGGCCG 2 cut(s) 558, 1359
Eco53kI GAGCTC 2 cut(s) 277, 1784
Eco57I CTGAAG 2 cut(s) 671, 1556
Eco81I CCTNAGG 1 cut(s) 2070
Eco88I CYCGRG 5 cut(s) 877, 886, 1157, 1711, 1801
Eco91I GGTNACC 1 cut(s) 890
EcoICRI GAGCTC 2 cut(s) 277, 1784
EcoO109I RGGNCCY 4 cut(s) 490, 805, 874, 1426
EcoO65I GGTNACC 1 cut(s) 890
EcoRI GAATTC 1 cut(s) 898
EcoRII CCWGG 3 cut(s) 801, 870, 943
EcoT14I CCWWGG 1 cut(s) 1603
EcoT38I GRGCYC 4 cut(s) 279, 809, 1429, 1786
EgeI GGCGCC 2 cut(s) 334, 1078
EheI GGCGCC 2 cut(s) 334, 1078
ErhI CCWWGG 1 cut(s) 1603
FaeI CATG 8 cut(s) 196, 1469, 1524, 1607, 1747, 2012, 2042, 2127
FalI AAGNNNNNCTT 2 cut(s) 1167, 1199
FaqI GGGAC 1 cut(s) 1784
FatI CATG 8 cut(s) 192, 1465, 1520, 1603, 1743, 2008, 2038, 2123
FauI CCCGC 8 cut(s) 227, 420, 441, 692, 833, 1115, 1161, 1484
FbaI TGATCA 3 cut(s) 951, 1446, 1683
FblI GTMKAC 2 cut(s) 111, 1983
FokI GGATG 6 cut(s) 251, 941, 1377, 1841, 1970, 2098
FriOI GRGCYC 4 cut(s) 279, 809, 1429, 1786
FspBI CTAG 5 cut(s) 1752, 1794, 1896, 1913, 2027
GlaI GCGC 6 cut(s) 237, 334, 437, 1078, 1557, 1732
GsaI CCCAGC 2 cut(s) 719, 1292
GsuI CTGGAG 4 cut(s) 425, 836, 1863, 1877
HaeII RGCGCY 3 cut(s) 336, 1080, 1734
HgaI GACGC 4 cut(s) 167, 211, 983, 1224
HhaI GCGC 6 cut(s) 238, 335, 438, 1079, 1558, 1733
Hin1I GRCGYC 4 cut(s) 159, 333, 975, 1077
Hin1II CATG 8 cut(s) 196, 1469, 1524, 1607, 1747, 2012, 2042, 2127
Hin6I GCGC 6 cut(s) 236, 333, 436, 1077, 1556, 1731
HinP1I GCGC 6 cut(s) 236, 333, 436, 1077, 1556, 1731
HincII GTYRAC 2 cut(s) 112, 1527
HindII GTYRAC 2 cut(s) 112, 1527
HphI GGTGA 9 cut(s) 193, 610, 680, 742, 748, 1324, 1414, 1678, 1951
Hpy166II GTNNAC 8 cut(s) 112, 120, 1012, 1056, 1206, 1276, 1527, 1984
Hpy8I GTNNAC 8 cut(s) 112, 120, 1012, 1056, 1206, 1276, 1527, 1984
Hpy99I CGWCG 9 cut(s) 80, 113, 161, 227, 230, 977, 1223, 1240, 1436
HpyAV CCTTC 4 cut(s) 145, 1024, 1169, 1629
HpyCH4III ACNGT 5 cut(s) 389, 503, 614, 736, 2095
HpyCH4IV ACGT 2 cut(s) 362, 1398
HpyCH4V TGCA 5 cut(s) 192, 514, 864, 1113, 1584
HpyF10VI GCNNNNNNNGC 7 cut(s) 131, 189, 977, 986, 1119, 1790, 1861
HpyF3I CTNAG 8 cut(s) 60, 286, 345, 1229, 1409, 1927, 2058, 2070
HpySE526I ACGT 2 cut(s) 362, 1398
Hsp92I GRCGYC 4 cut(s) 159, 333, 975, 1077
Hsp92II CATG 8 cut(s) 196, 1469, 1524, 1607, 1747, 2012, 2042, 2127
HspAI GCGC 6 cut(s) 236, 333, 436, 1077, 1556, 1731
KasI GGCGCC 2 cut(s) 332, 1076
Kpn2I TCCGGA 1 cut(s) 1786
KroI GCCGGC 1 cut(s) 125
KroNI GCCGGC 1 cut(s) 127
Ksp22I TGATCA 3 cut(s) 951, 1446, 1683
LguI GCTCTTC 1 cut(s) 356
LmnI GCTCC 5 cut(s) 134, 414, 855, 1762, 1789
Lsp1109I GCAGC 3 cut(s) 1212, 1596, 2042
LweI GCATC 2 cut(s) 1122, 1399
MaeI CTAG 5 cut(s) 1752, 1794, 1896, 1913, 2027
MaeII ACGT 2 cut(s) 362, 1398
MaeIII GTNAC 6 cut(s) 363, 389, 425, 730, 890, 1451
MbiI CCGCTC 1 cut(s) 850
MfeI CAATTG 2 cut(s) 509, 593
MflI RGATCY 2 cut(s) 911, 1539
MhlI GDGCHC 4 cut(s) 279, 809, 1429, 1786
MluCI AATT 8 cut(s) 509, 564, 593, 760, 774, 898, 1070, 1595
Mly113I GGCGCC 2 cut(s) 333, 1077
MmeI TCCRAC 7 cut(s) 165, 180, 483, 630, 1647, 1916, 2098
MroI TCCGGA 1 cut(s) 1786
MroNI GCCGGC 1 cut(s) 125
MseI TTAA 1 cut(s) 1544
MspA1I CMGCKG 1 cut(s) 768
MunI CAATTG 2 cut(s) 509, 593
Mva1269I GAATGC 2 cut(s) 989, 1586
MvaI CCWGG 3 cut(s) 803, 872, 945
MvnI CGCG 7 cut(s) 222, 236, 436, 815, 1358, 1491, 1558
MwoI GCNNNNNNNGC 7 cut(s) 131, 189, 977, 986, 1119, 1790, 1861
NaeI GCCGGC 1 cut(s) 127
NarI GGCGCC 2 cut(s) 333, 1077
NciI CCSGG 7 cut(s) 257, 557, 887, 888, 909, 1802, 1803
NcoI CCATGG 1 cut(s) 1603
NgoMIV GCCGGC 1 cut(s) 125
NheI GCTAGC 1 cut(s) 2026
NlaIII CATG 8 cut(s) 196, 1469, 1524, 1607, 1747, 2012, 2042, 2127
NlaIV GGNNCC 9 cut(s) 130, 334, 630, 807, 876, 913, 1078, 1427, 1428
NmeAIII GCCGAG 2 cut(s) 1387, 2106
NmuCI GTSAC 3 cut(s) 363, 730, 1451
NspV TTCGAA 1 cut(s) 797
PagI TCATGA 1 cut(s) 2123
PciSI GCTCTTC 1 cut(s) 356
PcsI WCGNNNNNNNCGW 2 cut(s) 1002, 1155
PctI GAATGC 2 cut(s) 989, 1586
PdiI GCCGGC 1 cut(s) 127
PfeI GAWTC 8 cut(s) 8, 207, 250, 703, 1300, 1486, 1643, 2104
PinAI ACCGGT 1 cut(s) 727
Ple19I CGATCG 1 cut(s) 1377
PluTI GGCGCC 2 cut(s) 336, 1080
Psp124BI GAGCTC 2 cut(s) 279, 1786
Psp6I CCWGG 3 cut(s) 801, 870, 943
PspEI GGTNACC 1 cut(s) 890
PspFI CCCAGC 2 cut(s) 715, 1288
PspGI CCWGG 3 cut(s) 801, 870, 943
PspN4I GGNNCC 9 cut(s) 130, 334, 630, 807, 876, 913, 1078, 1427, 1428
PspOMI GGGCCC 2 cut(s) 805, 1425
PsrI GAACNNNNNNTAC 2 cut(s) 1337, 1369
PstNI CAGNNNCTG 1 cut(s) 520
PsuI RGATCY 2 cut(s) 911, 1539
PvuI CGATCG 1 cut(s) 1377
RsaI GTAC 6 cut(s) 1057, 1277, 1346, 1418, 1900, 2056
RsaNI GTAC 6 cut(s) 1056, 1276, 1345, 1417, 1899, 2055
SacI GAGCTC 2 cut(s) 279, 1786
SalI GTCGAC 1 cut(s) 110
SapI GCTCTTC 1 cut(s) 356
SaqAI TTAA 1 cut(s) 1544
SduI GDGCHC 4 cut(s) 279, 809, 1429, 1786
SfaNI GCATC 2 cut(s) 1122, 1399
SfcI CTRYAG 1 cut(s) 1777
SfoI GGCGCC 2 cut(s) 334, 1078
SfuI TTCGAA 1 cut(s) 797
SinI GGWCC 3 cut(s) 177, 570, 598
SmaI CCCGGG 2 cut(s) 888, 1803
SpeI ACTAGT 1 cut(s) 1895
Sse9I AATT 8 cut(s) 509, 564, 593, 760, 774, 898, 1070, 1595
SspDI GGCGCC 2 cut(s) 332, 1076
SspMI CTAG 5 cut(s) 1752, 1794, 1896, 1913, 2027
SstI GAGCTC 2 cut(s) 279, 1786
StyI CCWWGG 1 cut(s) 1603
TaaI ACNGT 5 cut(s) 389, 503, 614, 736, 2095
TaiI ACGT 2 cut(s) 365, 1401
TasI AATT 8 cut(s) 509, 564, 593, 760, 774, 898, 1070, 1595
TatI WGTACW 2 cut(s) 1275, 2054
TauI GCSGC 7 cut(s) 301, 304, 771, 980, 983, 1335, 1361
TfiI GAWTC 8 cut(s) 8, 207, 250, 703, 1300, 1486, 1643, 2104
Tru1I TTAA 1 cut(s) 1544
Tru9I TTAA 1 cut(s) 1544
TscAI CASTG 2 cut(s) 550, 1456
TseFI GTSAC 3 cut(s) 363, 730, 1451
TseI GCWGC 3 cut(s) 1225, 1584, 2030
Tsp45I GTSAC 3 cut(s) 363, 730, 1451
TspDTI ATGAA 9 cut(s) 17, 209, 982, 1760, 1827, 1974, 1997, 2027, 2102
TspGWI ACGGA 3 cut(s) 93, 955, 1775
TspMI CCCGGG 2 cut(s) 886, 1801
TspRI CASTG 2 cut(s) 550, 1456
VpaK11BI GGWCC 3 cut(s) 177, 570, 598
XapI RAATTY 5 cut(s) 564, 774, 898, 1070, 1595
XcmI CCANNNNNNNNNTGG 1 cut(s) 1821
XmaI CCCGGG 2 cut(s) 886, 1801
XmiI GTMKAC 2 cut(s) 111, 1983
XspI CTAG 5 cut(s) 1752, 1794, 1896, 1913, 2027
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.