Rh4CG325500
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4C
OG0010877
Physical Location & Seq
Reverse (-)
58669976 .. 58674230
4255 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4CG325500.1

Sequence Viewer

Length: 2139 bp
ATGAAGGATTCCACTATTTACTCACTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTGTTTTGGCGACTTCGCTGAGTCTTTGACTCCCGACGGACTCTCCCTCCTCTCCCTCAAATCCGCCGTCGACCAGTCCACCGCCGGCTCCGCCTTCTCCGACTGGGCCGACTCCGACGCCTCGCCCTGCCGATGGACCGGCATTTCCTGCATGAACATCACCGGATTCCCCGACCCCCGCGTCGTCGGGATCGCGCTCTCCGGCAAGAATCTCCGGGGCTACATCCCCTCCGAGCTCGGAAACTTAGTCTATCTGCGGCGGCTCAACCTCCACAGCAACAACTTCTACGGCGCCATACCGACTCAGCTCTTCAATGCCACGTCACTCCACAGCCTCTTCCTCTACGGTAACAACCTCTCCGGTCAGCTCCCGCCCTCGATCTGTAACCTCCCGCGCCTCCAGAACCTCGATCTCTCCAACAACTCCCTCTCCGGTTCGTTAGACAAGGGCCTCAACAACTGTAAGCAATTGCAGAGACTGATACTCGCCGGAAACCATTTCACCGGCGAAATCCCGGCCGGAATTTGGTCCGGTATGGAAAACCTCCTCCAATTGGACCTCTCCGACAACGGTTTCACCGGATCGGTGCCGGAAGATTTCGGCGACCTGAAGTCTCTCTCCGGCACGCTCAACCTCTCGTTCAATCACTTCTCCGGCCGGATTCCGAAATCGCTGGGAGACTTACCGGTGACGGTGAGCTTCGATCTTCGCAACAATAATTTCAGCGGCGAAATTCCCCAAACGGGGTCGTTTTCGAACCAGGGCCCGACCGCGTTTCTCAACAACCCTCTCTTATGCGGGTTTCCGCTCCAGAAATCTTGCAAAGACCAGGCCCCGAGTTCCCCGGGTAACCCGAATTCCTCGCCCGGATCCGACAACAACCCGAAAAAGGGGTTGAGTCCGGGTCTGATCATCCTCATCTCCGTAGCCGACGCCGCCGCAGTCGCATTCATCGGTTTGGTCGTAGTTTACGTCTACTGGAAAAAGAAGGACAATGCCAACGGCTGTTCGTGTACCGGAAAGACAAAATTCGGCGCCAACACCGAGAAAAGCCGACTCTGTTGTTCTTGCATCAACGGCGGGTTCGGGAACTCGGACTCCGAACTCGACCCGCCCGAGAGAGGAAGAGGCGAAGGAGAGCTTGTGGCAATCGATAAGGGGTTCACATTTGAACTCGACGAGCTGCTGAGAGCGTCGGCTTATGTTCTGGGGAAGAGTGGGTTGGGGATTGTGTACAAGGTGGTGCTGGGGAATGGGATTCCGGTGGCGGTGAGGAGGTTGGGGGAGGGCGGCGATCAGAGGTACAAGGAGTTCGCGGCCGAGGTTCAGGCGATCGGTAGAGTGAAGCATCCCAACGTGGTGAGGCTGAGGGCGTACTATTGGGCCCCCGACGAGAAGCTCTTGATCAGTGATTTTATTTCGCATGGGAACTTGGCCTCTGCGATTCGCGGGAGAAATGGTCAGCCTATAAGCCTCTCATGGTCAACGAGGCTGAAGATCTTAAAGGGAACAGCGCGAGGCTTGGCCTATCTCCACGAATGCAGCCCCCGAAAATTTGTCCATGGAGACATCAAACCTTCCAACATTCTACTCGACAACGAATCTCAACCCTACATTTCGGATTTCGGACTCAACAGACTGATCACCATAACTGGCAACAACCCCTCCTCGGGTGGCTTGATGGGTAGCGCTCTCCCTTACATGAAAACTAGCCAAACGGAGCGAGCCAACAACTACAGAGCTCCGGAGGCTAGAGTCCCGGGTAATAAACCAACTCAAAAATGGGATGTTTATTCATTCGGAGTTGTCCTGCTGGAGTTGCTCACAGGAAAGTCTCCAGAGCTATCTCCAACTAGTACCTCAACTTCCCTAGAGATACCGGACTTAGTGAGGTGGGTGAGGAAAGGATTTGAGGATGAAAACCCTCTTTCGGATATGGTAGACCCTGTGTTGCTTCAAGAAGTTCATGCCAAAAAGGAAGTGCTAGCAGCTTTTCATGTAGCACTTGCTTGTACTGAGCCTGATCCTGAGGTTAGGCCGAGGATGAAAACTGTCTCCGAGAATCTCGAAAGGGTTGGATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

712

Amino Acids

77.26

Weight (kDa)

7.1

Isoelectric Point (pI)

31.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 29 - 68 8.5e-10 Leucine rich repeat N-terminal domain
LRR_14 PF23598 71 - 179 6.2e-07 Leucine-rich repeat region
LRR_8 PF13855 103 - 161 2.3e-06 Leucine rich repeat
LRR_8 PF13855 174 - 235 1.1e-07 Leucine rich repeat
Pkinase PF00069 420 - 650 1.1e-31 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 421 - 707 1.2e-28 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010877)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67510
fragaria_vesca FvH4_4g24010 FvH4_4g24010 FvH4_4g24010
malus_domestica MD13G1116600.v1.1 MD16G1117000.v1.1
prunus_persica Prupe.1G229200_v2.0.a1
pyrus_communis pycom13g10120 pycom16g10010
rosa_chinensis RchiOBHm_Chr4g0430671
rosa_laevigata RLG00000006957
rosa_multiflora Rmu_sc0000131.1_g000012 Rmu_sc0023719.1_g000001
rosa_roxburghii Rroxscaffold_5G00372410
rosa_rugosa Rorug04G0245100
rosa_samantha Rh4AG301200 Rh4BG308300 Rh4CG325500 Rh4DG305900
rosa_wichuraiana Rw4G026150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 233
AccB1I GGYRCC 3 cut(s) 344, 640, 1088
AccBSI CCGCTC 1 cut(s) 862
AccI GTMKAC 3 cut(s) 123, 1029, 1995
AccII CGCG 7 cut(s) 234, 248, 448, 827, 1370, 1503, 1570
AccIII TCCGGA 1 cut(s) 1798
AclWI GGATC 5 cut(s) 251, 643, 918, 931, 2072
AcoI YGGCCR 3 cut(s) 570, 709, 1371
AcsI RAATTY 5 cut(s) 576, 786, 910, 1082, 1607
AcuI CTGAAG 2 cut(s) 683, 1568
AcyI GRCGYC 4 cut(s) 171, 345, 987, 1089
AfaI GTAC 6 cut(s) 1069, 1289, 1358, 1430, 1912, 2068
AfeI AGCGCT 1 cut(s) 1744
AgeI ACCGGT 1 cut(s) 739
AgsI TTSAA 4 cut(s) 367, 697, 1226, 2012
AhdI GACNNNNNGTC 1 cut(s) 664
AhlI ACTAGT 1 cut(s) 1907
AjiI CACGTC 1 cut(s) 375
AjnI CCWGG 2 cut(s) 813, 882
AjuI GAANNNNNNNTTGG 2 cut(s) 1259, 1291
AloI GAACNNNNNNTCC 2 cut(s) 1136, 1168
Alw21I GWGCWC 2 cut(s) 291, 1798
Alw26I GTCTC 6 cut(s) 523, 672, 726, 1614, 1893, 2113
AlwI GGATC 5 cut(s) 251, 643, 918, 931, 2072
AlwNI CAGNNNCTG 1 cut(s) 532
Ama87I CYCGRG 5 cut(s) 889, 898, 1169, 1723, 1813
Aor13HI TCCGGA 1 cut(s) 1798
Aor51HI AGCGCT 1 cut(s) 1744
ApaI GGGCCC 2 cut(s) 821, 1441
ApeKI GCWGC 3 cut(s) 1237, 1596, 2042
ApoI RAATTY 5 cut(s) 576, 786, 910, 1082, 1607
ArsI GACNNNNNNTTYG 2 cut(s) 105, 137
AsiGI ACCGGT 1 cut(s) 739
AspLEI GCGC 6 cut(s) 250, 347, 450, 1091, 1570, 1745
AsuC2I CCSGG 8 cut(s) 269, 569, 899, 900, 921, 957, 1814, 1815
AsuHPI GGTGA 9 cut(s) 205, 547, 622, 754, 760, 1336, 1426, 1690, 1963
AsuII TTCGAA 1 cut(s) 809
AsuNHI GCTAGC 1 cut(s) 2038
AvaI CYCGRG 5 cut(s) 889, 898, 1169, 1723, 1813
AvaII GGWCC 3 cut(s) 189, 582, 610
AxyI CCTNAGG 1 cut(s) 2082
BaeGI GKGCMC 2 cut(s) 821, 1441
BamHI GGATCC 1 cut(s) 923
BanI GGYRCC 3 cut(s) 344, 640, 1088
BanII GRGCYC 4 cut(s) 291, 821, 1441, 1798
BarI GAAGNNNNNNTAC 2 cut(s) 1903, 1935
Bbv12I GWGCWC 2 cut(s) 291, 1798
BbvCI CCTCAGC 1 cut(s) 1421
BbvI GCAGC 3 cut(s) 1224, 1608, 2054
BccI CCATC 2 cut(s) 180, 1729
BceAI ACGGC 4 cut(s) 104, 358, 1072, 1147
BciT130I CCWGG 2 cut(s) 815, 884
BclI TGATCA 3 cut(s) 963, 1458, 1695
BcnI CCSGG 8 cut(s) 269, 569, 899, 900, 921, 957, 1814, 1815
BcoDI GTCTC 6 cut(s) 523, 672, 726, 1614, 1893, 2113
BcuI ACTAGT 1 cut(s) 1907
BfaI CTAG 5 cut(s) 1764, 1806, 1908, 1925, 2039
BfmI CTRYAG 1 cut(s) 1789
BfoI RGCGCY 3 cut(s) 348, 1092, 1746
BglII AGATCT 1 cut(s) 1551
Bme18I GGWCC 3 cut(s) 189, 582, 610
BmeRI GACNNNNNGTC 1 cut(s) 664
BmeT110I CYCGRG 5 cut(s) 889, 898, 1169, 1723, 1813
BmgBI CACGTC 1 cut(s) 375
BmiI GGNNCC 9 cut(s) 142, 346, 642, 819, 888, 925, 1090, 1439, 1440
BmrI ACTGGG 1 cut(s) 166
BmsI GCATC 2 cut(s) 1134, 1411
BmtI GCTAGC 1 cut(s) 2042
BmuI ACTGGG 1 cut(s) 166
BpmI CTGGAG 4 cut(s) 437, 848, 1875, 1889
Bpu10I CCTNAGC 1 cut(s) 1421
Bpu14I TTCGAA 1 cut(s) 809
BpuMI CCSGG 8 cut(s) 269, 569, 899, 900, 921, 957, 1814, 1815
Bsa29I ATCGAT 1 cut(s) 1206
BsaHI GRCGYC 4 cut(s) 171, 345, 987, 1089
BsaJI CCNNGG 9 cut(s) 268, 814, 897, 898, 1374, 1615, 1722, 1813, 2093
Bse118I RCCGGY 4 cut(s) 137, 191, 557, 739
Bse1I ACTGG 4 cut(s) 127, 161, 1037, 1711
Bse21I CCTNAGG 1 cut(s) 2082
BseAI TCCGGA 1 cut(s) 1798
BseBI CCWGG 2 cut(s) 815, 884
BseCI ATCGAT 1 cut(s) 1206
BseDI CCNNGG 9 cut(s) 268, 814, 897, 898, 1374, 1615, 1722, 1813, 2093
BseGI GGATG 6 cut(s) 276, 966, 1402, 1846, 1975, 2103
BseMII CTCAG 6 cut(s) 63, 371, 1232, 1412, 2061, 2073
BseNI ACTGG 4 cut(s) 127, 161, 1037, 1711
BseRI GAGGAG 4 cut(s) 92, 590, 1342, 1711
BseSI GKGCMC 2 cut(s) 821, 1441
BseX3I CGGCCG 3 cut(s) 570, 709, 1371
BseXI GCAGC 3 cut(s) 1224, 1608, 2054
BseYI CCCAGC 2 cut(s) 727, 1300
Bsh1236I CGCG 7 cut(s) 234, 248, 448, 827, 1370, 1503, 1570
Bsh1285I CGRYCG 5 cut(s) 573, 712, 825, 1374, 1389
BshNI GGYRCC 3 cut(s) 344, 640, 1088
BshTI ACCGGT 1 cut(s) 739
BshVI ATCGAT 1 cut(s) 1206
BsiEI CGRYCG 5 cut(s) 573, 712, 825, 1374, 1389
BsiHKAI GWGCWC 2 cut(s) 291, 1798
BsiHKCI CYCGRG 5 cut(s) 889, 898, 1169, 1723, 1813
BslFI GGGAC 1 cut(s) 1796
BsmAI GTCTC 6 cut(s) 523, 672, 726, 1614, 1893, 2113
BsmFI GGGAC 1 cut(s) 1796
BsmI GAATGC 2 cut(s) 1001, 1598
BsoBI CYCGRG 5 cut(s) 889, 898, 1169, 1723, 1813
Bsp119I TTCGAA 1 cut(s) 809
Bsp120I GGGCCC 2 cut(s) 817, 1437
Bsp1286I GDGCHC 4 cut(s) 291, 821, 1441, 1798
Bsp13I TCCGGA 1 cut(s) 1798
Bsp1407I TGTACA 1 cut(s) 1287
Bsp19I CCATGG 1 cut(s) 1615
BspCNI CTCAG 6 cut(s) 64, 370, 1233, 1413, 2062, 2074
BspDI ATCGAT 1 cut(s) 1206
BspEI TCCGGA 1 cut(s) 1798
BspFNI CGCG 7 cut(s) 234, 248, 448, 827, 1370, 1503, 1570
BspHI TCATGA 1 cut(s) 2135
BspLI GGNNCC 9 cut(s) 142, 346, 642, 819, 888, 925, 1090, 1439, 1440
BspOI GCTAGC 1 cut(s) 2042
BspPI GGATC 5 cut(s) 251, 643, 918, 931, 2072
BspQI GCTCTTC 1 cut(s) 368
BspT104I TTCGAA 1 cut(s) 809
BspT107I GGYRCC 3 cut(s) 344, 640, 1088
BsrBI CCGCTC 1 cut(s) 862
BsrFI RCCGGY 4 cut(s) 137, 191, 557, 739
BsrGI TGTACA 1 cut(s) 1287
BsrI ACTGG 4 cut(s) 127, 161, 1037, 1711
BssAI RCCGGY 4 cut(s) 137, 191, 557, 739
BssECI CCNNGG 9 cut(s) 268, 814, 897, 898, 1374, 1615, 1722, 1813, 2093
BssNI GRCGYC 4 cut(s) 171, 345, 987, 1089
BssT1I CCWWGG 1 cut(s) 1615
Bst2UI CCWGG 2 cut(s) 815, 884
Bst4CI ACNGT 5 cut(s) 401, 515, 626, 748, 2107
Bst6I CTCTTC 5 cut(s) 32, 368, 395, 1174, 1262
BstACI GRCGYC 4 cut(s) 171, 345, 987, 1089
BstAPI GCANNNNNTGC 1 cut(s) 201
BstAUI TGTACA 1 cut(s) 1287
BstBI TTCGAA 1 cut(s) 809
BstC8I GCNNGC 4 cut(s) 139, 680, 1779, 2040
BstDEI CTNAG 8 cut(s) 72, 298, 357, 1241, 1421, 1939, 2070, 2082
BstDSI CCRYGG 1 cut(s) 1615
BstEII GGTNACC 1 cut(s) 902
BstF5I GGATG 6 cut(s) 276, 966, 1402, 1846, 1975, 2103
BstFNI CGCG 7 cut(s) 234, 248, 448, 827, 1370, 1503, 1570
BstH2I RGCGCY 3 cut(s) 348, 1092, 1746
BstHHI GCGC 6 cut(s) 250, 347, 450, 1091, 1570, 1745
BstMAI GTCTC 6 cut(s) 523, 672, 726, 1614, 1893, 2113
BstMCI CGRYCG 5 cut(s) 573, 712, 825, 1374, 1389
BstMWI GCNNNNNNNGC 7 cut(s) 143, 201, 989, 998, 1131, 1802, 1873
BstNI CCWGG 2 cut(s) 815, 884
BstPI GGTNACC 1 cut(s) 902
BstSFI CTRYAG 1 cut(s) 1789
BstSLI GKGCMC 2 cut(s) 821, 1441
BstUI CGCG 7 cut(s) 234, 248, 448, 827, 1370, 1503, 1570
BstV1I GCAGC 3 cut(s) 1224, 1608, 2054
BstX2I RGATCY 2 cut(s) 923, 1551
BstYI RGATCY 2 cut(s) 923, 1551
BstZI CGGCCG 3 cut(s) 570, 709, 1371
Bsu15I ATCGAT 1 cut(s) 1206
Bsu36I CCTNAGG 1 cut(s) 2082
BsuTUI ATCGAT 1 cut(s) 1206
BtgI CCRYGG 1 cut(s) 1615
BtrI CACGTC 1 cut(s) 375
BtsCI GGATG 6 cut(s) 276, 966, 1402, 1846, 1975, 2103
BtsIMutI CAGTG 1 cut(s) 1468
Cac8I GCNNGC 4 cut(s) 139, 680, 1779, 2040
CaiI CAGNNNCTG 1 cut(s) 532
CciI TCATGA 1 cut(s) 2135
CfoI GCGC 6 cut(s) 250, 347, 450, 1091, 1570, 1745
Cfr10I RCCGGY 4 cut(s) 137, 191, 557, 739
Cfr9I CCCGGG 2 cut(s) 898, 1813
ClaI ATCGAT 1 cut(s) 1206
CseI GACGC 4 cut(s) 179, 223, 995, 1236
Csp6I GTAC 6 cut(s) 1068, 1288, 1357, 1429, 1911, 2067
CspAI ACCGGT 1 cut(s) 739
CviAII CATG 8 cut(s) 205, 1478, 1533, 1616, 1756, 2021, 2051, 2136
CviQI GTAC 6 cut(s) 1068, 1288, 1357, 1429, 1911, 2067
DdeI CTNAG 8 cut(s) 72, 298, 357, 1241, 1421, 1939, 2070, 2082
DinI GGCGCC 2 cut(s) 346, 1090
DrdI GACNNNNNNGTC 1 cut(s) 233
DriI GACNNNNNGTC 1 cut(s) 664
DseDI GACNNNNNNGTC 1 cut(s) 233
EaeI YGGCCR 3 cut(s) 570, 709, 1371
EagI CGGCCG 3 cut(s) 570, 709, 1371
Eam1104I CTCTTC 5 cut(s) 32, 368, 395, 1174, 1262
Eam1105I GACNNNNNGTC 1 cut(s) 664
EarI CTCTTC 5 cut(s) 32, 368, 395, 1174, 1262
EciI GGCGGA 2 cut(s) 106, 133
Ecl136II GAGCTC 2 cut(s) 289, 1796
EclXI CGGCCG 3 cut(s) 570, 709, 1371
Eco130I CCWWGG 1 cut(s) 1615
Eco24I GRGCYC 4 cut(s) 291, 821, 1441, 1798
Eco47I GGWCC 3 cut(s) 189, 582, 610
Eco47III AGCGCT 1 cut(s) 1744
Eco52I CGGCCG 3 cut(s) 570, 709, 1371
Eco53kI GAGCTC 2 cut(s) 289, 1796
Eco57I CTGAAG 2 cut(s) 683, 1568
Eco81I CCTNAGG 1 cut(s) 2082
Eco88I CYCGRG 5 cut(s) 889, 898, 1169, 1723, 1813
Eco91I GGTNACC 1 cut(s) 902
EcoICRI GAGCTC 2 cut(s) 289, 1796
EcoO109I RGGNCCY 4 cut(s) 502, 817, 886, 1438
EcoO65I GGTNACC 1 cut(s) 902
EcoRI GAATTC 1 cut(s) 910
EcoRII CCWGG 2 cut(s) 813, 882
EcoT14I CCWWGG 1 cut(s) 1615
EcoT38I GRGCYC 4 cut(s) 291, 821, 1441, 1798
EgeI GGCGCC 2 cut(s) 346, 1090
EheI GGCGCC 2 cut(s) 346, 1090
ErhI CCWWGG 1 cut(s) 1615
FaeI CATG 8 cut(s) 208, 1481, 1536, 1619, 1759, 2024, 2054, 2139
FalI AAGNNNNNCTT 2 cut(s) 1179, 1211
FaqI GGGAC 1 cut(s) 1796
FatI CATG 8 cut(s) 204, 1477, 1532, 1615, 1755, 2020, 2050, 2135
FauI CCCGC 7 cut(s) 239, 432, 453, 845, 1127, 1173, 1496
FbaI TGATCA 3 cut(s) 963, 1458, 1695
FblI GTMKAC 3 cut(s) 123, 1029, 1995
FokI GGATG 6 cut(s) 263, 953, 1389, 1853, 1982, 2110
FriOI GRGCYC 4 cut(s) 291, 821, 1441, 1798
FspBI CTAG 5 cut(s) 1764, 1806, 1908, 1925, 2039
GlaI GCGC 6 cut(s) 249, 346, 449, 1090, 1569, 1744
GsaI CCCAGC 2 cut(s) 731, 1304
GsuI CTGGAG 4 cut(s) 437, 848, 1875, 1889
HaeII RGCGCY 3 cut(s) 348, 1092, 1746
HgaI GACGC 4 cut(s) 179, 223, 995, 1236
HhaI GCGC 6 cut(s) 250, 347, 450, 1091, 1570, 1745
Hin1I GRCGYC 4 cut(s) 171, 345, 987, 1089
Hin1II CATG 8 cut(s) 208, 1481, 1536, 1619, 1759, 2024, 2054, 2139
Hin6I GCGC 6 cut(s) 248, 345, 448, 1089, 1568, 1743
HinP1I GCGC 6 cut(s) 248, 345, 448, 1089, 1568, 1743
HincII GTYRAC 2 cut(s) 124, 1539
HindII GTYRAC 2 cut(s) 124, 1539
HphI GGTGA 9 cut(s) 205, 547, 622, 754, 760, 1336, 1426, 1690, 1963
Hpy166II GTNNAC 9 cut(s) 124, 132, 1024, 1030, 1068, 1218, 1288, 1539, 1996
Hpy8I GTNNAC 9 cut(s) 124, 132, 1024, 1030, 1068, 1218, 1288, 1539, 1996
Hpy99I CGWCG 9 cut(s) 92, 125, 173, 239, 242, 989, 1235, 1252, 1448
HpyAV CCTTC 4 cut(s) 157, 1036, 1181, 1641
HpyCH4III ACNGT 5 cut(s) 401, 515, 626, 748, 2107
HpyCH4IV ACGT 3 cut(s) 374, 1026, 1410
HpyCH4V TGCA 5 cut(s) 204, 526, 876, 1125, 1596
HpyF10VI GCNNNNNNNGC 7 cut(s) 143, 201, 989, 998, 1131, 1802, 1873
HpyF3I CTNAG 8 cut(s) 72, 298, 357, 1241, 1421, 1939, 2070, 2082
HpySE526I ACGT 3 cut(s) 374, 1026, 1410
Hsp92I GRCGYC 4 cut(s) 171, 345, 987, 1089
Hsp92II CATG 8 cut(s) 208, 1481, 1536, 1619, 1759, 2024, 2054, 2139
HspAI GCGC 6 cut(s) 248, 345, 448, 1089, 1568, 1743
KasI GGCGCC 2 cut(s) 344, 1088
Kpn2I TCCGGA 1 cut(s) 1798
KroI GCCGGC 1 cut(s) 137
KroNI GCCGGC 1 cut(s) 139
Ksp22I TGATCA 3 cut(s) 963, 1458, 1695
LguI GCTCTTC 1 cut(s) 368
LmnI GCTCC 5 cut(s) 146, 426, 867, 1774, 1801
Lsp1109I GCAGC 3 cut(s) 1224, 1608, 2054
LweI GCATC 2 cut(s) 1134, 1411
MaeI CTAG 5 cut(s) 1764, 1806, 1908, 1925, 2039
MaeII ACGT 3 cut(s) 374, 1026, 1410
MaeIII GTNAC 5 cut(s) 375, 401, 437, 742, 902
MbiI CCGCTC 1 cut(s) 862
MfeI CAATTG 2 cut(s) 521, 605
MflI RGATCY 2 cut(s) 923, 1551
MhlI GDGCHC 4 cut(s) 291, 821, 1441, 1798
MluCI AATT 8 cut(s) 521, 576, 605, 772, 786, 910, 1082, 1607
Mly113I GGCGCC 2 cut(s) 345, 1089
MmeI TCCRAC 8 cut(s) 177, 192, 495, 642, 951, 1659, 1928, 2110
MroI TCCGGA 1 cut(s) 1798
MroNI GCCGGC 1 cut(s) 137
MseI TTAA 1 cut(s) 1556
MspA1I CMGCKG 1 cut(s) 780
MunI CAATTG 2 cut(s) 521, 605
Mva1269I GAATGC 2 cut(s) 1001, 1598
MvaI CCWGG 2 cut(s) 815, 884
MvnI CGCG 7 cut(s) 234, 248, 448, 827, 1370, 1503, 1570
MwoI GCNNNNNNNGC 7 cut(s) 143, 201, 989, 998, 1131, 1802, 1873
NaeI GCCGGC 1 cut(s) 139
NarI GGCGCC 2 cut(s) 345, 1089
NciI CCSGG 8 cut(s) 269, 569, 899, 900, 921, 957, 1814, 1815
NcoI CCATGG 1 cut(s) 1615
NgoMIV GCCGGC 1 cut(s) 137
NheI GCTAGC 1 cut(s) 2038
NlaIII CATG 8 cut(s) 208, 1481, 1536, 1619, 1759, 2024, 2054, 2139
NlaIV GGNNCC 9 cut(s) 142, 346, 642, 819, 888, 925, 1090, 1439, 1440
NmeAIII GCCGAG 2 cut(s) 1399, 2118
NmuCI GTSAC 2 cut(s) 375, 742
NspV TTCGAA 1 cut(s) 809
PagI TCATGA 1 cut(s) 2135
PciSI GCTCTTC 1 cut(s) 368
PcsI WCGNNNNNNNCGW 3 cut(s) 1014, 1023, 1167
PctI GAATGC 2 cut(s) 1001, 1598
PdiI GCCGGC 1 cut(s) 139
PfeI GAWTC 8 cut(s) 8, 219, 262, 715, 1312, 1498, 1655, 2116
PinAI ACCGGT 1 cut(s) 739
Ple19I CGATCG 1 cut(s) 1389
PluTI GGCGCC 2 cut(s) 348, 1092
Psp124BI GAGCTC 2 cut(s) 291, 1798
Psp6I CCWGG 2 cut(s) 813, 882
PspEI GGTNACC 1 cut(s) 902
PspFI CCCAGC 2 cut(s) 727, 1300
PspGI CCWGG 2 cut(s) 813, 882
PspN4I GGNNCC 9 cut(s) 142, 346, 642, 819, 888, 925, 1090, 1439, 1440
PspOMI GGGCCC 2 cut(s) 817, 1437
PsrI GAACNNNNNNTAC 2 cut(s) 1349, 1381
PstNI CAGNNNCTG 1 cut(s) 532
PsuI RGATCY 2 cut(s) 923, 1551
PvuI CGATCG 1 cut(s) 1389
RsaI GTAC 6 cut(s) 1069, 1289, 1358, 1430, 1912, 2068
RsaNI GTAC 6 cut(s) 1068, 1288, 1357, 1429, 1911, 2067
SacI GAGCTC 2 cut(s) 291, 1798
SalI GTCGAC 1 cut(s) 122
SapI GCTCTTC 1 cut(s) 368
SaqAI TTAA 1 cut(s) 1556
SduI GDGCHC 4 cut(s) 291, 821, 1441, 1798
SfaNI GCATC 2 cut(s) 1134, 1411
SfcI CTRYAG 1 cut(s) 1789
SfoI GGCGCC 2 cut(s) 346, 1090
SfuI TTCGAA 1 cut(s) 809
SgrAI CRCCGGYG 1 cut(s) 557
SinI GGWCC 3 cut(s) 189, 582, 610
SmaI CCCGGG 2 cut(s) 900, 1815
SpeI ACTAGT 1 cut(s) 1907
Sse9I AATT 8 cut(s) 521, 576, 605, 772, 786, 910, 1082, 1607
SspDI GGCGCC 2 cut(s) 344, 1088
SspMI CTAG 5 cut(s) 1764, 1806, 1908, 1925, 2039
SstI GAGCTC 2 cut(s) 291, 1798
StyI CCWWGG 1 cut(s) 1615
TaaI ACNGT 5 cut(s) 401, 515, 626, 748, 2107
TaiI ACGT 3 cut(s) 377, 1029, 1413
TasI AATT 8 cut(s) 521, 576, 605, 772, 786, 910, 1082, 1607
TatI WGTACW 2 cut(s) 1287, 2066
TauI GCSGC 7 cut(s) 313, 316, 783, 992, 995, 1347, 1373
TfiI GAWTC 8 cut(s) 8, 219, 262, 715, 1312, 1498, 1655, 2116
Tru1I TTAA 1 cut(s) 1556
Tru9I TTAA 1 cut(s) 1556
TscAI CASTG 1 cut(s) 1468
TseFI GTSAC 2 cut(s) 375, 742
TseI GCWGC 3 cut(s) 1237, 1596, 2042
Tsp45I GTSAC 2 cut(s) 375, 742
TspDTI ATGAA 9 cut(s) 17, 221, 994, 1772, 1839, 1986, 2009, 2039, 2114
TspGWI ACGGA 3 cut(s) 105, 967, 1787
TspMI CCCGGG 2 cut(s) 898, 1813
TspRI CASTG 1 cut(s) 1468
VpaK11BI GGWCC 3 cut(s) 189, 582, 610
XapI RAATTY 5 cut(s) 576, 786, 910, 1082, 1607
XcmI CCANNNNNNNNNTGG 1 cut(s) 1833
XmaI CCCGGG 2 cut(s) 898, 1813
XmiI GTMKAC 3 cut(s) 123, 1029, 1995
XspI CTAG 5 cut(s) 1764, 1806, 1908, 1925, 2039
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.