Rroxscaffold_5G00372410
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0010877
Physical Location & Seq
Reverse (-)
53843248 .. 53848337
5090 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00372410.1

Sequence Viewer

Length: 1935 bp
ATGAACATCACCGGATTCCCCGACCCCCGCGTCGTCGGGGTCGCCCTCTCCGGCAAGAATCTCCGGGGCTACATCCCCTCCGAGCTCGGAAACTTAGTCTATCTGCGGCGGCTCAACCTCCACAGCAACAACTTCTACGGCGCCATTCCGACTCAGCTCTTCAATGCCACGTCACTCCACAGCCTCTTCCTCTACGGTAACAACCTCTCCGGTCAGCTCCCGCCTTCGATCTGTAACCTCCCGCGCCTCCAGAACCTCGACCTCTCCAACAACTCCCTCTCCGGTTCGTTAGACAAGGGCCTCAACAACTGTAAGCAATTGCAGAGACTGATACTCGCCGGAAACCATTTCACCGGCGAAATCCCTGCCGGAATTTGGTCCGGTATGGAAAACCTCCTCCAATTGGACCTCTCCGACAACGGTTTCACCGGATCGGTGCCGGAAGATTTCGGCGACCTGAAGTCTCTCTCCGGCACGCTCAACCTCTCGTTCAATCACTTATCCGGCAAGATTCCGAAATCGCTGGGAGACTTACCGGTGACGGTGAGCTTCGATCTCCGCAACAATAATTTCAGCGGCGAAATTCCCCAAACGGGGTCGTTTTCGAACCAGGGCCCGACCGCGTTTCTCAACAACCCTCTCTTATGCGGGTTTCCGCTCCAGAAATCTTGCAAAGACCAGGCCCCGAGTTCCCCGGGTAACCCGAATTCCTCGCCCGGATCCGACAACAACCCGAAAAAGGGGTTGAGTCCGGGTCTGATCATCCTCATCTCCGTAGCCGACGCCGCCGCAGTCGCATTCATCGGTTTGGTCGTAGTTTACATTTACTGGAAAAAGAAGGACAATGCCAACGGCTGTTCATGTACCGGAAAGACAAAATTCGGCGCGAACACCGAGAAAAACCGACTCTGTTGTTCTTGCATCAACGGCGGGTTCGGGAACTCGGACTCCGAACTCGACCCGCCCGAGAGAGGAAGAGGCGAAGGAGAGCTTGTGGCAATCGATAAGGGGTTCACATTTGAACTCGACGAGCTGCTGAGAGCGTCGGCTTATGTTCTGGGGAAGAGTGGGTTGGGGATTGTGTACAAGGTGGTGCTGGGGAATGGGATTCCTGTGGCGGTGAGGAGGTTGGGGGAGGGCGGCGATCAGAGGTACAAGGAGTTCGCGACGGAGGTTCAGGCGATCGGTAGAGTGAAGCATCCCAACGTGGTGAGGCTGAGGGCGTACTATTGGGCCCCCGACGAGAAGCTCTTGATCAGTGACTTTATTTCGCATGGGAACTTGGCCTCTGCGATTCGCGGGAGGAATGGTCAGCCTATAAGCCTCTCATGGTCAACGAGGCTGAAGATCTTAAAGGGAGCAGCGCGAGGCTTGGCCTATCTCCACGAATGCAGCCCCCGAAAATTTGTCCATGGAGACATCAAACCTTCCAACATTCTACTCGACAACGAATCTCAACCCTACATTTCGGATTTCGGACTCAACAGACTGATCACCATCACTGGCAACAACCCCTCCTCGGGTGGCTTGATGGGTAGCGCTCTCCCTTACATGAAATCTAGTCAAACGGAGCGAGCCAACAACTACAGAGCTCCGGAGGCTAGAGTCCCTGGTAATAAACCAACTCAAAAATGGGATGTTTATTCATTCGGAGTTGTCCTGCTGGAGTTGCTCACAGGAAAGTCTCCGGAGCTATCTCCAACTAGTACCTCAACTTCCCTAGAGATACCGGACTTGGTGAGGTGGGTGAGGAAAGGATTTGAGGATGAAAACCCTCTTTCGGATATGGTAGACCCTGTGTTGCTTCAAGAAGTTCATGCCAAAAAGGAAGTGCTAGCAGCTTTTCATGTAGCACTTGCTTGTACTGAGCCTGATCCTGAGGTTAGGCCGAGGATGAAAACTGTCTCCGAGAATCTCGAAAGGGTTGGATCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

644

Amino Acids

69.69

Weight (kDa)

8.49

Isoelectric Point (pI)

31.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 20 - 112 1.4e-06 Leucine-rich repeat region
LRR_8 PF13855 35 - 93 2e-06 Leucine rich repeat
LRR_8 PF13855 106 - 167 5.8e-08 Leucine rich repeat
Pkinase PF00069 352 - 582 3.3e-31 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 353 - 639 9.3e-29 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010877)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67510
fragaria_vesca FvH4_4g24010 FvH4_4g24010 FvH4_4g24010
malus_domestica MD13G1116600.v1.1 MD16G1117000.v1.1
prunus_persica Prupe.1G229200_v2.0.a1
pyrus_communis pycom13g10120 pycom16g10010
rosa_chinensis RchiOBHm_Chr4g0430671
rosa_laevigata RLG00000006957
rosa_multiflora Rmu_sc0000131.1_g000012 Rmu_sc0023719.1_g000001
rosa_roxburghii Rroxscaffold_5G00372410
rosa_rugosa Rorug04G0245100
rosa_samantha Rh4AG301200 Rh4BG308300 Rh4CG325500 Rh4DG305900
rosa_wichuraiana Rw4G026150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 29
AccB1I GGYRCC 2 cut(s) 140, 436
AccBSI CCGCTC 1 cut(s) 658
AccI GTMKAC 1 cut(s) 1791
AccII CGCG 7 cut(s) 30, 244, 623, 887, 1166, 1299, 1366
AccIII TCCGGA 2 cut(s) 1594, 1687
AclWI GGATC 4 cut(s) 439, 714, 727, 1868
AcsI RAATTY 5 cut(s) 372, 582, 706, 878, 1403
AcuI CTGAAG 2 cut(s) 479, 1364
AcyI GRCGYC 2 cut(s) 141, 783
AfaI GTAC 6 cut(s) 865, 1085, 1154, 1226, 1708, 1864
AfeI AGCGCT 1 cut(s) 1540
AgeI ACCGGT 1 cut(s) 535
AgsI TTSAA 4 cut(s) 163, 493, 1022, 1808
AhdI GACNNNNNGTC 1 cut(s) 460
AhlI ACTAGT 1 cut(s) 1703
AjiI CACGTC 1 cut(s) 171
AjnI CCWGG 3 cut(s) 609, 678, 1609
AjuI GAANNNNNNNTTGG 2 cut(s) 1055, 1087
AloI GAACNNNNNNTCC 2 cut(s) 932, 964
Alw21I GWGCWC 2 cut(s) 87, 1594
Alw26I GTCTC 6 cut(s) 319, 468, 522, 1410, 1689, 1909
AlwI GGATC 4 cut(s) 439, 714, 727, 1868
AlwNI CAGNNNCTG 1 cut(s) 328
Ama87I CYCGRG 4 cut(s) 685, 694, 965, 1519
Aor13HI TCCGGA 2 cut(s) 1594, 1687
Aor51HI AGCGCT 1 cut(s) 1540
AoxI GGCC 7 cut(s) 298, 613, 681, 1233, 1284, 1374, 1886
ApaI GGGCCC 2 cut(s) 617, 1237
ApeKI GCWGC 4 cut(s) 1033, 1361, 1392, 1838
ApoI RAATTY 5 cut(s) 372, 582, 706, 878, 1403
AsiGI ACCGGT 1 cut(s) 535
AspLEI GCGC 5 cut(s) 143, 246, 887, 1366, 1541
AspS9I GGNCC 8 cut(s) 298, 378, 406, 613, 614, 682, 1233, 1234
AsuC2I CCSGG 5 cut(s) 65, 695, 696, 717, 753
AsuHPI GGTGA 9 cut(s) 343, 418, 550, 556, 1132, 1222, 1486, 1750, 1759
AsuII TTCGAA 1 cut(s) 605
AsuNHI GCTAGC 1 cut(s) 1834
AvaI CYCGRG 4 cut(s) 685, 694, 965, 1519
AvaII GGWCC 2 cut(s) 378, 406
AxyI CCTNAGG 1 cut(s) 1878
BaeGI GKGCMC 2 cut(s) 617, 1237
BamHI GGATCC 1 cut(s) 719
BanI GGYRCC 2 cut(s) 140, 436
BanII GRGCYC 4 cut(s) 87, 617, 1237, 1594
BarI GAAGNNNNNNTAC 2 cut(s) 1699, 1731
Bbv12I GWGCWC 2 cut(s) 87, 1594
BbvCI CCTCAGC 1 cut(s) 1217
BbvI GCAGC 4 cut(s) 1020, 1373, 1404, 1850
BccI CCATC 2 cut(s) 1505, 1525
BceAI ACGGC 3 cut(s) 154, 868, 943
BcgI CGANNNNNNTGC 2 cut(s) 347, 381
BciT130I CCWGG 3 cut(s) 611, 680, 1611
BclI TGATCA 3 cut(s) 759, 1254, 1491
BcnI CCSGG 5 cut(s) 65, 695, 696, 717, 753
BcoDI GTCTC 6 cut(s) 319, 468, 522, 1410, 1689, 1909
BcuI ACTAGT 1 cut(s) 1703
BfaI CTAG 5 cut(s) 1560, 1602, 1704, 1721, 1835
BfmI CTRYAG 1 cut(s) 1585
BfoI RGCGCY 2 cut(s) 144, 1542
BglII AGATCT 1 cut(s) 1347
Bme1390I CCNGG 8 cut(s) 65, 611, 680, 695, 696, 717, 753, 1611
Bme18I GGWCC 2 cut(s) 378, 406
BmeRI GACNNNNNGTC 1 cut(s) 460
BmeT110I CYCGRG 4 cut(s) 685, 694, 965, 1519
BmgBI CACGTC 1 cut(s) 171
BmgT120I GGNCC 8 cut(s) 298, 378, 406, 613, 614, 682, 1233, 1234
BmiI GGNNCC 7 cut(s) 142, 438, 615, 684, 721, 1235, 1236
BmrFI CCNGG 8 cut(s) 65, 611, 680, 695, 696, 717, 753, 1611
BmsI GCATC 2 cut(s) 930, 1207
BmtI GCTAGC 1 cut(s) 1838
BpmI CTGGAG 3 cut(s) 233, 644, 1685
Bpu10I CCTNAGC 1 cut(s) 1217
Bpu14I TTCGAA 1 cut(s) 605
BpuMI CCSGG 5 cut(s) 65, 695, 696, 717, 753
Bsa29I ATCGAT 1 cut(s) 1002
BsaBI GATNNNNATC 1 cut(s) 1496
BsaHI GRCGYC 2 cut(s) 141, 783
BsaJI CCNNGG 8 cut(s) 64, 610, 693, 694, 1411, 1518, 1609, 1889
Bse118I RCCGGY 2 cut(s) 353, 535
Bse1I ACTGG 2 cut(s) 833, 1507
Bse21I CCTNAGG 1 cut(s) 1878
Bse8I GATNNNNATC 1 cut(s) 1496
BseAI TCCGGA 2 cut(s) 1594, 1687
BseBI CCWGG 3 cut(s) 611, 680, 1611
BseCI ATCGAT 1 cut(s) 1002
BseDI CCNNGG 8 cut(s) 64, 610, 693, 694, 1411, 1518, 1609, 1889
BseGI GGATG 6 cut(s) 72, 762, 1198, 1642, 1771, 1899
BseJI GATNNNNATC 1 cut(s) 1496
BseMII CTCAG 5 cut(s) 167, 1028, 1208, 1857, 1869
BseNI ACTGG 2 cut(s) 833, 1507
BseRI GAGGAG 3 cut(s) 386, 1138, 1507
BseSI GKGCMC 2 cut(s) 617, 1237
BseXI GCAGC 4 cut(s) 1020, 1373, 1404, 1850
BseYI CCCAGC 2 cut(s) 523, 1096
Bsh1236I CGCG 7 cut(s) 30, 244, 623, 887, 1166, 1299, 1366
Bsh1285I CGRYCG 2 cut(s) 621, 1185
BshFI GGCC 7 cut(s) 300, 615, 683, 1235, 1286, 1376, 1888
BshNI GGYRCC 2 cut(s) 140, 436
BshTI ACCGGT 1 cut(s) 535
BshVI ATCGAT 1 cut(s) 1002
BsiEI CGRYCG 2 cut(s) 621, 1185
BsiHKAI GWGCWC 2 cut(s) 87, 1594
BsiHKCI CYCGRG 4 cut(s) 685, 694, 965, 1519
BslFI GGGAC 1 cut(s) 1592
BsmAI GTCTC 6 cut(s) 319, 468, 522, 1410, 1689, 1909
BsmFI GGGAC 1 cut(s) 1592
BsmI GAATGC 2 cut(s) 797, 1394
BsnI GGCC 7 cut(s) 300, 615, 683, 1235, 1286, 1376, 1888
BsoBI CYCGRG 4 cut(s) 685, 694, 965, 1519
Bsp119I TTCGAA 1 cut(s) 605
Bsp120I GGGCCC 2 cut(s) 613, 1233
Bsp1286I GDGCHC 4 cut(s) 87, 617, 1237, 1594
Bsp13I TCCGGA 2 cut(s) 1594, 1687
Bsp1407I TGTACA 1 cut(s) 1083
Bsp19I CCATGG 1 cut(s) 1411
Bsp68I TCGCGA 1 cut(s) 1166
BspANI GGCC 7 cut(s) 300, 615, 683, 1235, 1286, 1376, 1888
BspCNI CTCAG 5 cut(s) 166, 1029, 1209, 1858, 1870
BspDI ATCGAT 1 cut(s) 1002
BspEI TCCGGA 2 cut(s) 1594, 1687
BspFNI CGCG 7 cut(s) 30, 244, 623, 887, 1166, 1299, 1366
BspHI TCATGA 1 cut(s) 1931
BspLI GGNNCC 7 cut(s) 142, 438, 615, 684, 721, 1235, 1236
BspOI GCTAGC 1 cut(s) 1838
BspPI GGATC 4 cut(s) 439, 714, 727, 1868
BspQI GCTCTTC 1 cut(s) 164
BspT104I TTCGAA 1 cut(s) 605
BspT107I GGYRCC 2 cut(s) 140, 436
BsrBI CCGCTC 1 cut(s) 658
BsrFI RCCGGY 2 cut(s) 353, 535
BsrGI TGTACA 1 cut(s) 1083
BsrI ACTGG 2 cut(s) 833, 1507
BssAI RCCGGY 2 cut(s) 353, 535
BssECI CCNNGG 8 cut(s) 64, 610, 693, 694, 1411, 1518, 1609, 1889
BssNI GRCGYC 2 cut(s) 141, 783
BssT1I CCWWGG 1 cut(s) 1411
Bst2UI CCWGG 3 cut(s) 611, 680, 1611
Bst4CI ACNGT 5 cut(s) 197, 311, 422, 544, 1903
Bst6I CTCTTC 4 cut(s) 164, 191, 970, 1058
BstACI GRCGYC 2 cut(s) 141, 783
BstAUI TGTACA 1 cut(s) 1083
BstBI TTCGAA 1 cut(s) 605
BstC8I GCNNGC 3 cut(s) 476, 1575, 1836
BstDEI CTNAG 6 cut(s) 94, 153, 1037, 1217, 1866, 1878
BstDSI CCRYGG 1 cut(s) 1411
BstEII GGTNACC 1 cut(s) 698
BstF5I GGATG 6 cut(s) 72, 762, 1198, 1642, 1771, 1899
BstFNI CGCG 7 cut(s) 30, 244, 623, 887, 1166, 1299, 1366
BstH2I RGCGCY 2 cut(s) 144, 1542
BstHHI GCGC 5 cut(s) 143, 246, 887, 1366, 1541
BstMAI GTCTC 6 cut(s) 319, 468, 522, 1410, 1689, 1909
BstMCI CGRYCG 2 cut(s) 621, 1185
BstMWI GCNNNNNNNGC 5 cut(s) 785, 794, 927, 1598, 1669
BstNI CCWGG 3 cut(s) 611, 680, 1611
BstPI GGTNACC 1 cut(s) 698
BstSCI CCNGG 8 cut(s) 63, 609, 678, 693, 694, 715, 751, 1609
BstSFI CTRYAG 1 cut(s) 1585
BstSLI GKGCMC 2 cut(s) 617, 1237
BstUI CGCG 7 cut(s) 30, 244, 623, 887, 1166, 1299, 1366
BstV1I GCAGC 4 cut(s) 1020, 1373, 1404, 1850
BstX2I RGATCY 2 cut(s) 719, 1347
BstYI RGATCY 2 cut(s) 719, 1347
Bsu15I ATCGAT 1 cut(s) 1002
Bsu36I CCTNAGG 1 cut(s) 1878
BsuRI GGCC 7 cut(s) 300, 615, 683, 1235, 1286, 1376, 1888
BsuTUI ATCGAT 1 cut(s) 1002
BtgI CCRYGG 1 cut(s) 1411
BtrI CACGTC 1 cut(s) 171
BtsCI GGATG 6 cut(s) 72, 762, 1198, 1642, 1771, 1899
BtsIMutI CAGTG 2 cut(s) 1264, 1500
BtuMI TCGCGA 1 cut(s) 1166
Cac8I GCNNGC 3 cut(s) 476, 1575, 1836
CaiI CAGNNNCTG 1 cut(s) 328
CciI TCATGA 1 cut(s) 1931
CfoI GCGC 5 cut(s) 143, 246, 887, 1366, 1541
Cfr10I RCCGGY 2 cut(s) 353, 535
Cfr13I GGNCC 8 cut(s) 298, 378, 406, 613, 614, 682, 1233, 1234
Cfr9I CCCGGG 1 cut(s) 694
ClaI ATCGAT 1 cut(s) 1002
CseI GACGC 3 cut(s) 19, 791, 1032
Csp6I GTAC 6 cut(s) 864, 1084, 1153, 1225, 1707, 1863
CspAI ACCGGT 1 cut(s) 535
CviAII CATG 8 cut(s) 861, 1274, 1329, 1412, 1552, 1817, 1847, 1932
CviQI GTAC 6 cut(s) 864, 1084, 1153, 1225, 1707, 1863
DdeI CTNAG 6 cut(s) 94, 153, 1037, 1217, 1866, 1878
DinI GGCGCC 1 cut(s) 142
DrdI GACNNNNNNGTC 1 cut(s) 29
DriI GACNNNNNGTC 1 cut(s) 460
DseDI GACNNNNNNGTC 1 cut(s) 29
Eam1104I CTCTTC 4 cut(s) 164, 191, 970, 1058
Eam1105I GACNNNNNGTC 1 cut(s) 460
EarI CTCTTC 4 cut(s) 164, 191, 970, 1058
Ecl136II GAGCTC 2 cut(s) 85, 1592
Eco130I CCWWGG 1 cut(s) 1411
Eco24I GRGCYC 4 cut(s) 87, 617, 1237, 1594
Eco47I GGWCC 2 cut(s) 378, 406
Eco47III AGCGCT 1 cut(s) 1540
Eco53kI GAGCTC 2 cut(s) 85, 1592
Eco57I CTGAAG 2 cut(s) 479, 1364
Eco81I CCTNAGG 1 cut(s) 1878
Eco88I CYCGRG 4 cut(s) 685, 694, 965, 1519
Eco91I GGTNACC 1 cut(s) 698
EcoICRI GAGCTC 2 cut(s) 85, 1592
EcoO109I RGGNCCY 4 cut(s) 298, 613, 682, 1234
EcoO65I GGTNACC 1 cut(s) 698
EcoRI GAATTC 1 cut(s) 706
EcoRII CCWGG 3 cut(s) 609, 678, 1609
EcoT14I CCWWGG 1 cut(s) 1411
EcoT38I GRGCYC 4 cut(s) 87, 617, 1237, 1594
EgeI GGCGCC 1 cut(s) 142
EheI GGCGCC 1 cut(s) 142
ErhI CCWWGG 1 cut(s) 1411
FaeI CATG 8 cut(s) 864, 1277, 1332, 1415, 1555, 1820, 1850, 1935
FalI AAGNNNNNCTT 2 cut(s) 975, 1007
FaqI GGGAC 1 cut(s) 1592
FatI CATG 8 cut(s) 860, 1273, 1328, 1411, 1551, 1816, 1846, 1931
FauI CCCGC 7 cut(s) 35, 228, 249, 641, 923, 969, 1292
FbaI TGATCA 3 cut(s) 759, 1254, 1491
FblI GTMKAC 1 cut(s) 1791
FokI GGATG 6 cut(s) 59, 749, 1185, 1649, 1778, 1906
FriOI GRGCYC 4 cut(s) 87, 617, 1237, 1594
FspBI CTAG 5 cut(s) 1560, 1602, 1704, 1721, 1835
GlaI GCGC 5 cut(s) 142, 245, 886, 1365, 1540
GsaI CCCAGC 2 cut(s) 527, 1100
GsuI CTGGAG 3 cut(s) 233, 644, 1685
HaeII RGCGCY 2 cut(s) 144, 1542
HaeIII GGCC 7 cut(s) 300, 615, 683, 1235, 1286, 1376, 1888
HgaI GACGC 3 cut(s) 19, 791, 1032
HhaI GCGC 5 cut(s) 143, 246, 887, 1366, 1541
Hin1I GRCGYC 2 cut(s) 141, 783
Hin1II CATG 8 cut(s) 864, 1277, 1332, 1415, 1555, 1820, 1850, 1935
Hin6I GCGC 5 cut(s) 141, 244, 885, 1364, 1539
HinP1I GCGC 5 cut(s) 141, 244, 885, 1364, 1539
HincII GTYRAC 1 cut(s) 1335
HindII GTYRAC 1 cut(s) 1335
HphI GGTGA 9 cut(s) 343, 418, 550, 556, 1132, 1222, 1486, 1750, 1759
Hpy166II GTNNAC 5 cut(s) 820, 1014, 1084, 1335, 1792
Hpy8I GTNNAC 5 cut(s) 820, 1014, 1084, 1335, 1792
Hpy99I CGWCG 7 cut(s) 35, 38, 785, 1031, 1048, 1171, 1244
HpyAV CCTTC 4 cut(s) 234, 832, 977, 1437
HpyCH4III ACNGT 5 cut(s) 197, 311, 422, 544, 1903
HpyCH4IV ACGT 2 cut(s) 170, 1206
HpyCH4V TGCA 4 cut(s) 322, 672, 921, 1392
HpyF10VI GCNNNNNNNGC 5 cut(s) 785, 794, 927, 1598, 1669
HpyF3I CTNAG 6 cut(s) 94, 153, 1037, 1217, 1866, 1878
HpySE526I ACGT 2 cut(s) 170, 1206
Hsp92I GRCGYC 2 cut(s) 141, 783
Hsp92II CATG 8 cut(s) 864, 1277, 1332, 1415, 1555, 1820, 1850, 1935
HspAI GCGC 5 cut(s) 141, 244, 885, 1364, 1539
KasI GGCGCC 1 cut(s) 140
Kpn2I TCCGGA 2 cut(s) 1594, 1687
Ksp22I TGATCA 3 cut(s) 759, 1254, 1491
LguI GCTCTTC 1 cut(s) 164
LmnI GCTCC 6 cut(s) 222, 663, 1358, 1570, 1597, 1690
Lsp1109I GCAGC 4 cut(s) 1020, 1373, 1404, 1850
LweI GCATC 2 cut(s) 930, 1207
MaeI CTAG 5 cut(s) 1560, 1602, 1704, 1721, 1835
MaeII ACGT 2 cut(s) 170, 1206
MaeIII GTNAC 6 cut(s) 171, 197, 233, 538, 698, 1259
MbiI CCGCTC 1 cut(s) 658
MboII GAAGA 6 cut(s) 151, 178, 455, 987, 1075, 1357
MfeI CAATTG 2 cut(s) 317, 401
MflI RGATCY 2 cut(s) 719, 1347
MhlI GDGCHC 4 cut(s) 87, 617, 1237, 1594
MluCI AATT 8 cut(s) 317, 372, 401, 568, 582, 706, 878, 1403
Mly113I GGCGCC 1 cut(s) 141
MlyI GAGTC 6 cut(s) 145, 757, 900, 941, 1473, 1614
MmeI TCCRAC 7 cut(s) 173, 291, 438, 747, 1455, 1724, 1906
MroI TCCGGA 2 cut(s) 1594, 1687
MseI TTAA 1 cut(s) 1352
MspA1I CMGCKG 1 cut(s) 576
MspR9I CCNGG 8 cut(s) 65, 611, 680, 695, 696, 717, 753, 1611
MunI CAATTG 2 cut(s) 317, 401
Mva1269I GAATGC 2 cut(s) 797, 1394
MvaI CCWGG 3 cut(s) 611, 680, 1611
MvnI CGCG 7 cut(s) 30, 244, 623, 887, 1166, 1299, 1366
MwoI GCNNNNNNNGC 5 cut(s) 785, 794, 927, 1598, 1669
NarI GGCGCC 1 cut(s) 141
NciI CCSGG 5 cut(s) 65, 695, 696, 717, 753
NcoI CCATGG 1 cut(s) 1411
NheI GCTAGC 1 cut(s) 1834
NlaIII CATG 8 cut(s) 864, 1277, 1332, 1415, 1555, 1820, 1850, 1935
NlaIV GGNNCC 7 cut(s) 142, 438, 615, 684, 721, 1235, 1236
NmeAIII GCCGAG 1 cut(s) 1914
NmuCI GTSAC 3 cut(s) 171, 538, 1259
NruI TCGCGA 1 cut(s) 1166
NspV TTCGAA 1 cut(s) 605
PagI TCATGA 1 cut(s) 1931
PciSI GCTCTTC 1 cut(s) 164
PcsI WCGNNNNNNNCGW 2 cut(s) 810, 963
PctI GAATGC 2 cut(s) 797, 1394
PfeI GAWTC 7 cut(s) 15, 58, 511, 1108, 1294, 1451, 1912
PinAI ACCGGT 1 cut(s) 535
Ple19I CGATCG 1 cut(s) 1185
PleI GAGTC 6 cut(s) 145, 756, 900, 941, 1473, 1613
PluTI GGCGCC 1 cut(s) 144
PpsI GAGTC 6 cut(s) 145, 756, 900, 941, 1473, 1613
Psp124BI GAGCTC 2 cut(s) 87, 1594
Psp6I CCWGG 3 cut(s) 609, 678, 1609
PspEI GGTNACC 1 cut(s) 698
PspFI CCCAGC 2 cut(s) 523, 1096
PspGI CCWGG 3 cut(s) 609, 678, 1609
PspN4I GGNNCC 7 cut(s) 142, 438, 615, 684, 721, 1235, 1236
PspOMI GGGCCC 2 cut(s) 613, 1233
PspPI GGNCC 8 cut(s) 298, 378, 406, 613, 614, 682, 1233, 1234
PsrI GAACNNNNNNTAC 2 cut(s) 1145, 1177
PstNI CAGNNNCTG 1 cut(s) 328
PsuI RGATCY 2 cut(s) 719, 1347
PvuI CGATCG 1 cut(s) 1185
RruI TCGCGA 1 cut(s) 1166
RsaI GTAC 6 cut(s) 865, 1085, 1154, 1226, 1708, 1864
RsaNI GTAC 6 cut(s) 864, 1084, 1153, 1225, 1707, 1863
SacI GAGCTC 2 cut(s) 87, 1594
SapI GCTCTTC 1 cut(s) 164
SaqAI TTAA 1 cut(s) 1352
Sau96I GGNCC 8 cut(s) 298, 378, 406, 613, 614, 682, 1233, 1234
SchI GAGTC 6 cut(s) 145, 757, 900, 941, 1473, 1614
ScrFI CCNGG 8 cut(s) 65, 611, 680, 695, 696, 717, 753, 1611
SduI GDGCHC 4 cut(s) 87, 617, 1237, 1594
SfaNI GCATC 2 cut(s) 930, 1207
SfcI CTRYAG 1 cut(s) 1585
SfoI GGCGCC 1 cut(s) 142
SfuI TTCGAA 1 cut(s) 605
SgrAI CRCCGGYG 1 cut(s) 353
SinI GGWCC 2 cut(s) 378, 406
SmaI CCCGGG 1 cut(s) 696
SpeI ACTAGT 1 cut(s) 1703
Sse9I AATT 8 cut(s) 317, 372, 401, 568, 582, 706, 878, 1403
SspDI GGCGCC 1 cut(s) 140
SspMI CTAG 5 cut(s) 1560, 1602, 1704, 1721, 1835
SstI GAGCTC 2 cut(s) 87, 1594
StyD4I CCNGG 8 cut(s) 63, 609, 678, 693, 694, 715, 751, 1609
StyI CCWWGG 1 cut(s) 1411
TaaI ACNGT 5 cut(s) 197, 311, 422, 544, 1903
TaiI ACGT 2 cut(s) 173, 1209
TaqI TCGA 9 cut(s) 227, 258, 552, 605, 957, 1002, 1026, 1443, 1917
TasI AATT 8 cut(s) 317, 372, 401, 568, 582, 706, 878, 1403
TatI WGTACW 2 cut(s) 1083, 1862
TauI GCSGC 6 cut(s) 109, 112, 579, 788, 791, 1143
TfiI GAWTC 7 cut(s) 15, 58, 511, 1108, 1294, 1451, 1912
Tru1I TTAA 1 cut(s) 1352
Tru9I TTAA 1 cut(s) 1352
TscAI CASTG 2 cut(s) 1264, 1507
TseFI GTSAC 3 cut(s) 171, 538, 1259
TseI GCWGC 4 cut(s) 1033, 1361, 1392, 1838
Tsp45I GTSAC 3 cut(s) 171, 538, 1259
TspDTI ATGAA 9 cut(s) 17, 790, 849, 1568, 1635, 1782, 1805, 1835, 1910
TspGWI ACGGA 3 cut(s) 763, 1184, 1583
TspMI CCCGGG 1 cut(s) 694
TspRI CASTG 2 cut(s) 1264, 1507
VpaK11BI GGWCC 2 cut(s) 378, 406
XapI RAATTY 5 cut(s) 372, 582, 706, 878, 1403
XcmI CCANNNNNNNNNTGG 1 cut(s) 1629
XmaI CCCGGG 1 cut(s) 694
XmiI GTMKAC 1 cut(s) 1791
XspI CTAG 5 cut(s) 1560, 1602, 1704, 1721, 1835
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.