MD16G1117000.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0010877
Physical Location & Seq
Forward (+)
8293320 .. 8298152
4833 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1117000.v1.1.491

Sequence Viewer

Length: 2154 bp
ATGGAACCTTCCATGTTCTCCGCCCTCATCTTCCTCCTCTTCCTCGTCGACACCACACTCTCGCTCACCCCCGACGGCCTTTCCCTCCTCTCGCTCAAATCCGCCGTCGACCAGTCCTCCGACGGCTCCGCCTTCTCCGACTGGCTCGACACCGACCCCACCCCCTGCCGCTGGACCGGCATTTCCTGCATGAACATCACCGGCTTTTCCGACCCCCGCGTTGTTGGAATCACTCTCTCCGGCAAGAACCTCCGCGGATACATTCCCTCCGAGCTTGGAACATTAGTATACCTTCGCCGCCTCAACCTCCATAGCAACAACTTCCGTGGCTCAATCCCTTCCCAACTCTTCAACGCCACCTCACTCCACAGCCTCTTCCTCTACGGTAACAACCTCTCTGGTCCTCTCCCGCCGTCGATATGCAACCCCCCGCGCCTCCAGAACATCGACCTCTCCAACAACTCCCTCTCCGGTCCGCTCCAGAAGGACTACTTGAAGAACTGCCGCCAATTGCAGCGGCTGATCCTCGCGAGAAACCGATTCGAAGGCGAAATTCCGGCGGGAATTTGGTCAGACATGGAGAATCTAATCCAATTGGACCTCTCCTCCAACGAGTTAACCGGATCGGTGCCGGACGATTTCGGCAAGTTGAAGTCTCTCTCCGGCACGCTCAACTTATCGTTCAATCACTTATCGGGAAAGATTCCGAAATCCCTCGGCGAATTGCCTGTCACGGTGAGCTTTGATCTCCGAAACAATAATTTCAGCGGCGAGATTCCTCAAACGGGTTCGTTTTCGAACCAGGGTCCCACCGCGTTTCTGAACAATCCTCTGCTCTGCGGGTTTCCGCTTCAGAAAGCTTGTAAAAACCCGGGGCAGAGCTCCGCGGATAGTCCGAATTCGGGACCGGGTTCGGAAAAAAGTCCGGGAAAGGGATTGAGTCCGGGTCTGATTGTCCTAATCTCGGTGGCGGACGCCGCCGGAGTGGCGTTTATCGGATTAGTCGTCGTTTACATTTACTGGAAAAAGAAGGACGACGAGGATGGATGCAGTTGTACCGGAAAGAGCAATTTTGGCAGCAACGAGAAGCGCCACCTCTGTAATCTCTGCCAGCTCTGTTCTTGCGCTTGCATCAATGGCGGGTTCGGGAACGAGGATTCGGATTCCGGCGACCCGGAAAAGGGAGAGAGGGGGAAAGGGGAGGGGGATCTCGTTGCGATCGACAAGGGTTTCACGTTCGAGCTCGACGAGCTGTTGAGAGCATCGGCTTACGTGCTGGGGAAGAGCGGGTTGGGGATTGTGTACAAAGTGGTGCTCGGGAATGGAATCCCGGTGGCGGTGAGGAGGTTGGGGGAGGGCGGCGATCAGCGGTATAAGGAGTTCGCGGCGGAGATTCAGGCGATCGGGCGGGTGAAGCATCCGAATGTGGTGAAGCTGAGGGCGTACTATTGGGCGGCCGACGAGAAGCTTCTCATCAGTGACTTCATTTCTAACGGTAACTTGGCCTCTGCACTCCGCGGGAGAAACGGTCAGTCATCATCAAGCCTCTCGTGGACAACGAGGCTAAAGATCGCCAAGGGAACAGCCCGAGGCCTCGCCTACCTGCACGAATGCAGCCCCAGAAAGTTTGTCCACGGTGACATTAAACCCTCCAACATTCTTCTCGACAACGAATCTCAAGCCTACATTTCAGATTTCGGACTCAACAGGCTTATCGAAATCACAGGAAACAACCCCTCCTCGGGTGGCTTCATGGGCGGGGCTTTGCCTTACATGAAACCAGTACCAACAGAGCGATCAAACAATTACCGTGCCCCGGAGGCCCGTGTCACGGGAAGCAAACTCACCCAAAAATGGGACGTGTACTCATTCGGAGTTGTGTTGCTTGAATTGCTCACAGGGAAGTCCCCGGAGCTCTCTCCGACCACGTCAACTTCGGTCGAGGTCCCAGACCTAGTGAGGTGGGTGAGGAAAGGATTTGAGGATGAAAACCCTTTGTCGGATATGGTAGACCCTGTGTTGCTACAGGAAGTGCATGCCAAAAAGGAGGTACTTGCAGCTTTCCATGTAGCCCTTGCATGCACTGAGCCGGACCCTGAGGTTAGGCCAAGGATGAAATCCGTCTCTGAAAATCTTGAAAGGATTGGAACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

718

Amino Acids

77.62

Weight (kDa)

6.37

Isoelectric Point (pI)

34.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 24 - 63 4.1e-09 Leucine rich repeat N-terminal domain
LRR_8 PF13855 169 - 231 7.7e-09 Leucine rich repeat
Pkinase PF00069 425 - 648 2.8e-32 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 426 - 712 5.9e-31 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010877)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G67510
fragaria_vesca FvH4_4g24010 FvH4_4g24010 FvH4_4g24010
malus_domestica MD13G1116600.v1.1 MD16G1117000.v1.1
prunus_persica Prupe.1G229200_v2.0.a1
pyrus_communis pycom13g10120 pycom16g10010
rosa_chinensis RchiOBHm_Chr4g0430671
rosa_laevigata RLG00000006957
rosa_multiflora Rmu_sc0000131.1_g000012 Rmu_sc0023719.1_g000001
rosa_roxburghii Rroxscaffold_5G00372410
rosa_rugosa Rorug04G0245100
rosa_samantha Rh4AG301200 Rh4BG308300 Rh4CG325500 Rh4DG305900
rosa_wichuraiana Rw4G026150

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1611
AccB1I GGYRCC 1 cut(s) 628
AccBSI CCGCTC 2 cut(s) 478, 1287
AccI GTMKAC 4 cut(s) 48, 108, 288, 2010
AccII CGCG 8 cut(s) 219, 255, 433, 530, 815, 887, 1385, 1518
AclWI GGATC 3 cut(s) 517, 631, 1215
AcoI YGGCCR 1 cut(s) 1455
AcsI RAATTY 3 cut(s) 552, 564, 898
AcuI CTGAAG 1 cut(s) 836
AcyI GRCGYC 1 cut(s) 975
AfaI GTAC 6 cut(s) 1057, 1304, 1445, 1785, 1865, 2052
AflIII ACRYGT 1 cut(s) 1860
AgsI TTSAA 6 cut(s) 352, 496, 652, 685, 1889, 2138
AjiI CACGTC 2 cut(s) 1861, 1929
AjnI CCWGG 1 cut(s) 801
AjuI GAANNNNNNNTTGG 2 cut(s) 1274, 1306
Alw21I GWGCWC 4 cut(s) 884, 1245, 1317, 1917
Alw26I GTCTC 2 cut(s) 660, 2128
AlwI GGATC 3 cut(s) 517, 631, 1215
AlwNI CAGNNNCTG 1 cut(s) 520
Ama87I CYCGRG 4 cut(s) 871, 1316, 1587, 1741
AoxI GGCC 6 cut(s) 76, 1455, 1503, 1591, 1821, 2105
ApeKI GCWGC 4 cut(s) 514, 1077, 1614, 2057
ApoI RAATTY 3 cut(s) 552, 564, 898
ArsI GACNNNNNNTTYG 4 cut(s) 90, 122, 714, 746
AspLEI GCGC 3 cut(s) 435, 1092, 1127
AspS9I GGNCC 9 cut(s) 174, 401, 473, 598, 806, 905, 1822, 1945, 2092
AsuC2I CCSGG 9 cut(s) 872, 873, 909, 927, 945, 1175, 1331, 1817, 1910
AsuHPI GGTGA 9 cut(s) 58, 190, 748, 1351, 1423, 1441, 1649, 1837, 1978
AsuII TTCGAA 2 cut(s) 543, 797
AvaI CYCGRG 4 cut(s) 871, 1316, 1587, 1741
AvaII GGWCC 8 cut(s) 174, 401, 473, 598, 806, 905, 1945, 2092
AxyI CCTNAGG 1 cut(s) 2097
BaeGI GKGCMC 1 cut(s) 1816
BanI GGYRCC 1 cut(s) 628
BanII GRGCYC 3 cut(s) 884, 1245, 1917
BauI CACGAG 1 cut(s) 1549
Bbv12I GWGCWC 4 cut(s) 884, 1245, 1317, 1917
BbvCI CCTCAGC 1 cut(s) 1436
BbvI GCAGC 4 cut(s) 526, 1089, 1626, 2069
BccI CCATC 1 cut(s) 1037
BceAI ACGGC 4 cut(s) 89, 91, 139, 397
BciT130I CCWGG 1 cut(s) 803
BciVI GTATCC 1 cut(s) 251
BcnI CCSGG 9 cut(s) 872, 873, 909, 927, 945, 1175, 1331, 1817, 1910
BcoDI GTCTC 2 cut(s) 660, 2128
BfaI CTAG 1 cut(s) 1955
BfmI CTRYAG 1 cut(s) 2024
BfoI RGCGCY 1 cut(s) 1093
BfuAI ACCTGC 1 cut(s) 1611
BfuI GTATCC 1 cut(s) 251
BglI GCCNNNNNGGC 2 cut(s) 986, 1820
Bme18I GGWCC 8 cut(s) 174, 401, 473, 598, 806, 905, 1945, 2092
BmeT110I CYCGRG 4 cut(s) 871, 1316, 1587, 1741
BmgBI CACGTC 2 cut(s) 1861, 1929
BmgT120I GGNCC 9 cut(s) 174, 401, 473, 598, 806, 905, 1822, 1945, 2092
BmiI GGNNCC 8 cut(s) 6, 127, 630, 807, 808, 906, 1947, 2094
BmsI GCATC 4 cut(s) 1037, 1140, 1271, 1426
BpmI CTGGAG 2 cut(s) 422, 464
Bpu10I CCTNAGC 1 cut(s) 1436
Bpu14I TTCGAA 2 cut(s) 543, 797
BpuEI CTTGAG 1 cut(s) 1662
BpuMI CCSGG 9 cut(s) 872, 873, 909, 927, 945, 1175, 1331, 1817, 1910
BsaAI YACGTR 1 cut(s) 1273
BsaHI GRCGYC 1 cut(s) 975
BsaWI WCCGGW 3 cut(s) 470, 620, 1058
Bse118I RCCGGY 2 cut(s) 176, 200
Bse1I ACTGG 4 cut(s) 112, 146, 1025, 1781
Bse21I CCTNAGG 1 cut(s) 2097
BseBI CCWGG 1 cut(s) 803
BseGI GGATG 5 cut(s) 1048, 1052, 1417, 1990, 2118
BseMII CTCAG 3 cut(s) 1427, 2076, 2088
BseNI ACTGG 4 cut(s) 112, 146, 1025, 1781
BseRI GAGGAG 5 cut(s) 26, 77, 595, 1357, 1729
BseSI GKGCMC 1 cut(s) 1816
BseX3I CGGCCG 1 cut(s) 1455
BseXI GCAGC 4 cut(s) 526, 1089, 1626, 2069
BseYI CCCAGC 1 cut(s) 1276
BsgI GTGCAG 2 cut(s) 1494, 1589
Bsh1236I CGCG 8 cut(s) 219, 255, 433, 530, 815, 887, 1385, 1518
Bsh1285I CGRYCG 4 cut(s) 1221, 1404, 1458, 1941
BshFI GGCC 6 cut(s) 78, 1457, 1505, 1593, 1823, 2107
BshNI GGYRCC 1 cut(s) 628
BsiEI CGRYCG 4 cut(s) 1221, 1404, 1458, 1941
BsiHKAI GWGCWC 4 cut(s) 884, 1245, 1317, 1917
BsiHKCI CYCGRG 4 cut(s) 871, 1316, 1587, 1741
BslFI GGGAC 5 cut(s) 792, 918, 1871, 1891, 1931
BsmAI GTCTC 2 cut(s) 660, 2128
BsmBI CGTCTC 1 cut(s) 2128
BsmFI GGGAC 5 cut(s) 792, 918, 1871, 1891, 1931
BsmI GAATGC 1 cut(s) 1616
BsnI GGCC 6 cut(s) 78, 1457, 1505, 1593, 1823, 2107
BsoBI CYCGRG 4 cut(s) 871, 1316, 1587, 1741
Bsp119I TTCGAA 2 cut(s) 543, 797
Bsp1286I GDGCHC 5 cut(s) 884, 1245, 1317, 1816, 1917
Bsp1407I TGTACA 1 cut(s) 1302
Bsp143I GATC 9 cut(s) 522, 623, 745, 1207, 1218, 1363, 1401, 1569, 1796
Bsp68I TCGCGA 1 cut(s) 530
BspANI GGCC 6 cut(s) 78, 1457, 1505, 1593, 1823, 2107
BspCNI CTCAG 3 cut(s) 1428, 2077, 2089
BspFNI CGCG 8 cut(s) 219, 255, 433, 530, 815, 887, 1385, 1518
BspLI GGNNCC 8 cut(s) 6, 127, 630, 807, 808, 906, 1947, 2094
BspMI ACCTGC 1 cut(s) 1611
BspPI GGATC 3 cut(s) 517, 631, 1215
BspQI GCTCTTC 1 cut(s) 1277
BspT104I TTCGAA 2 cut(s) 543, 797
BspT107I GGYRCC 1 cut(s) 628
BsrBI CCGCTC 2 cut(s) 478, 1287
BsrFI RCCGGY 2 cut(s) 176, 200
BsrGI TGTACA 1 cut(s) 1302
BsrI ACTGG 4 cut(s) 112, 146, 1025, 1781
BssAI RCCGGY 2 cut(s) 176, 200
BssMI GATC 9 cut(s) 522, 623, 745, 1207, 1218, 1363, 1401, 1569, 1796
BssNAI GTATAC 1 cut(s) 289
BssNI GRCGYC 1 cut(s) 975
BssSI CACGAG 1 cut(s) 1549
BssT1I CCWWGG 2 cut(s) 1575, 2108
Bst1107I GTATAC 1 cut(s) 289
Bst2BI CACGAG 1 cut(s) 1549
Bst2UI CCWGG 1 cut(s) 803
Bst4CI ACNGT 6 cut(s) 386, 736, 1496, 1529, 1637, 1811
Bst6I CTCTTC 4 cut(s) 44, 353, 380, 1277
BstACI GRCGYC 1 cut(s) 975
BstAPI GCANNNNNTGC 1 cut(s) 186
BstAUI TGTACA 1 cut(s) 1302
BstBAI YACGTR 1 cut(s) 1273
BstBI TTCGAA 2 cut(s) 543, 797
BstC8I GCNNGC 5 cut(s) 668, 1112, 1129, 2037, 2080
BstDEI CTNAG 3 cut(s) 1436, 2085, 2097
BstDSI CCRYGG 5 cut(s) 253, 325, 885, 1516, 1633
BstF5I GGATG 5 cut(s) 1048, 1052, 1417, 1990, 2118
BstFNI CGCG 8 cut(s) 219, 255, 433, 530, 815, 887, 1385, 1518
BstH2I RGCGCY 1 cut(s) 1093
BstHHI GCGC 3 cut(s) 435, 1092, 1127
BstKTI GATC 9 cut(s) 525, 626, 748, 1210, 1221, 1366, 1404, 1572, 1799
BstMAI GTCTC 2 cut(s) 660, 2128
BstMBI GATC 9 cut(s) 522, 623, 745, 1207, 1218, 1363, 1401, 1569, 1796
BstMCI CGRYCG 4 cut(s) 1221, 1404, 1458, 1941
BstNI CCWGG 1 cut(s) 803
BstNSI RCATGY 2 cut(s) 2039, 2082
BstSFI CTRYAG 1 cut(s) 2024
BstSLI GKGCMC 1 cut(s) 1816
BstUI CGCG 8 cut(s) 219, 255, 433, 530, 815, 887, 1385, 1518
BstV1I GCAGC 4 cut(s) 526, 1089, 1626, 2069
BstX2I RGATCY 1 cut(s) 1207
BstYI RGATCY 1 cut(s) 1207
BstZ17I GTATAC 1 cut(s) 289
BstZI CGGCCG 1 cut(s) 1455
Bsu36I CCTNAGG 1 cut(s) 2097
BsuI GTATCC 1 cut(s) 251
BsuRI GGCC 6 cut(s) 78, 1457, 1505, 1593, 1823, 2107
BtgI CCRYGG 5 cut(s) 253, 325, 885, 1516, 1633
BtrI CACGTC 2 cut(s) 1861, 1929
BtsCI GGATG 5 cut(s) 1048, 1052, 1417, 1990, 2118
BtsIMutI CAGTG 2 cut(s) 1483, 2082
BtuMI TCGCGA 1 cut(s) 530
BveI ACCTGC 1 cut(s) 1611
Cac8I GCNNGC 5 cut(s) 668, 1112, 1129, 2037, 2080
CaiI CAGNNNCTG 1 cut(s) 520
CfoI GCGC 3 cut(s) 435, 1092, 1127
Cfr10I RCCGGY 2 cut(s) 176, 200
Cfr13I GGNCC 9 cut(s) 174, 401, 473, 598, 806, 905, 1822, 1945, 2092
Cfr42I CCGCGG 3 cut(s) 256, 888, 1519
Cfr9I CCCGGG 1 cut(s) 871
CpoI CGGWCCG 1 cut(s) 473
CseI GACGC 1 cut(s) 983
Csp6I GTAC 6 cut(s) 1056, 1303, 1444, 1784, 1864, 2051
CspCI CAANNNNNGTGG 2 cut(s) 307, 342
CspI CGGWCCG 1 cut(s) 473
CviAII CATG 8 cut(s) 13, 190, 577, 1753, 1774, 2036, 2066, 2079
CviQI GTAC 6 cut(s) 1056, 1303, 1444, 1784, 1864, 2051
DdeI CTNAG 3 cut(s) 1436, 2085, 2097
DpnI GATC 9 cut(s) 524, 625, 747, 1209, 1220, 1365, 1403, 1571, 1798
DpnII GATC 9 cut(s) 522, 623, 745, 1207, 1218, 1363, 1401, 1569, 1796
EaeI YGGCCR 1 cut(s) 1455
EagI CGGCCG 1 cut(s) 1455
Eam1104I CTCTTC 4 cut(s) 44, 353, 380, 1277
EarI CTCTTC 4 cut(s) 44, 353, 380, 1277
EciI GGCGGA 5 cut(s) 10, 91, 118, 986, 1403
Ecl136II GAGCTC 3 cut(s) 882, 1243, 1915
EclXI CGGCCG 1 cut(s) 1455
Eco130I CCWWGG 2 cut(s) 1575, 2108
Eco147I AGGCCT 1 cut(s) 1593
Eco24I GRGCYC 3 cut(s) 884, 1245, 1917
Eco47I GGWCC 8 cut(s) 174, 401, 473, 598, 806, 905, 1945, 2092
Eco52I CGGCCG 1 cut(s) 1455
Eco53kI GAGCTC 3 cut(s) 882, 1243, 1915
Eco57I CTGAAG 1 cut(s) 836
Eco81I CCTNAGG 1 cut(s) 2097
Eco88I CYCGRG 4 cut(s) 871, 1316, 1587, 1741
EcoICRI GAGCTC 3 cut(s) 882, 1243, 1915
EcoO109I RGGNCCY 2 cut(s) 806, 1945
EcoRI GAATTC 1 cut(s) 898
EcoRII CCWGG 1 cut(s) 801
EcoT14I CCWWGG 2 cut(s) 1575, 2108
EcoT38I GRGCYC 3 cut(s) 884, 1245, 1917
ErhI CCWWGG 2 cut(s) 1575, 2108
Esp3I CGTCTC 1 cut(s) 2128
FaeI CATG 8 cut(s) 16, 193, 580, 1756, 1777, 2039, 2069, 2082
FalI AAGNNNNNCTT 2 cut(s) 476, 508
FaqI GGGAC 5 cut(s) 792, 918, 1871, 1891, 1931
FatI CATG 8 cut(s) 12, 189, 576, 1752, 1773, 2035, 2065, 2078
FblI GTMKAC 4 cut(s) 48, 108, 288, 2010
FokI GGATG 5 cut(s) 1055, 1059, 1404, 1997, 2125
FriOI GRGCYC 3 cut(s) 884, 1245, 1917
FspBI CTAG 1 cut(s) 1955
GlaI GCGC 3 cut(s) 434, 1091, 1126
GsaI CCCAGC 1 cut(s) 1280
GsuI CTGGAG 2 cut(s) 422, 464
HaeII RGCGCY 1 cut(s) 1093
HaeIII GGCC 6 cut(s) 78, 1457, 1505, 1593, 1823, 2107
HgaI GACGC 1 cut(s) 983
HhaI GCGC 3 cut(s) 435, 1092, 1127
Hin1I GRCGYC 1 cut(s) 975
Hin1II CATG 8 cut(s) 16, 193, 580, 1756, 1777, 2039, 2069, 2082
Hin6I GCGC 3 cut(s) 433, 1090, 1125
HinP1I GCGC 3 cut(s) 433, 1090, 1125
HincII GTYRAC 4 cut(s) 49, 109, 618, 1932
HindII GTYRAC 4 cut(s) 49, 109, 618, 1932
HindIII AAGCTT 2 cut(s) 858, 1466
HpaI GTTAAC 1 cut(s) 618
HphI GGTGA 9 cut(s) 58, 190, 748, 1351, 1423, 1441, 1649, 1837, 1978
Hpy188III TCNNGA 9 cut(s) 439, 481, 529, 696, 903, 1147, 1318, 1664, 2135
Hpy99I CGWCG 9 cut(s) 50, 77, 110, 125, 418, 1010, 1040, 1250, 1463
HpyAV CCTTC 7 cut(s) 18, 142, 302, 348, 478, 539, 1024
HpyCH4III ACNGT 6 cut(s) 386, 736, 1496, 1529, 1637, 1811
HpyCH4IV ACGT 5 cut(s) 1235, 1272, 1860, 1928, 2150
HpyF3I CTNAG 3 cut(s) 1436, 2085, 2097
HpySE526I ACGT 5 cut(s) 1235, 1272, 1860, 1928, 2150
Hsp92I GRCGYC 1 cut(s) 975
Hsp92II CATG 8 cut(s) 16, 193, 580, 1756, 1777, 2039, 2069, 2082
HspAI GCGC 3 cut(s) 433, 1090, 1125
KflI GGGWCCC 1 cut(s) 806
KspAI GTTAAC 1 cut(s) 618
KspI CCGCGG 3 cut(s) 256, 888, 1519
Kzo9I GATC 9 cut(s) 522, 623, 745, 1207, 1218, 1363, 1401, 1569, 1796
LguI GCTCTTC 1 cut(s) 1277
LmnI GCTCC 4 cut(s) 131, 483, 887, 1912
Lsp1109I GCAGC 4 cut(s) 526, 1089, 1626, 2069
LweI GCATC 4 cut(s) 1037, 1140, 1271, 1426
MaeI CTAG 1 cut(s) 1955
MaeII ACGT 5 cut(s) 1235, 1272, 1860, 1928, 2150
MaeIII GTNAC 6 cut(s) 386, 730, 1478, 1496, 1637, 1828
MalI GATC 9 cut(s) 524, 625, 747, 1209, 1220, 1365, 1403, 1571, 1798
MbiI CCGCTC 2 cut(s) 478, 1287
MboI GATC 9 cut(s) 522, 623, 745, 1207, 1218, 1363, 1401, 1569, 1796
MboII GAAGA 7 cut(s) 22, 31, 340, 367, 508, 1294, 1652
MfeI CAATTG 2 cut(s) 509, 593
MflI RGATCY 1 cut(s) 1207
MhlI GDGCHC 5 cut(s) 884, 1245, 1317, 1816, 1917
MlyI GAGTC 2 cut(s) 949, 1695
MmeI TCCRAC 9 cut(s) 144, 162, 205, 234, 480, 633, 1677, 1946, 1980
MseI TTAA 2 cut(s) 617, 1644
MslI CAYNNNNRTG 1 cut(s) 1422
MspA1I CMGCKG 7 cut(s) 171, 255, 517, 768, 887, 1369, 1518
MunI CAATTG 2 cut(s) 509, 593
Mva1269I GAATGC 1 cut(s) 1616
MvaI CCWGG 1 cut(s) 803
MvnI CGCG 8 cut(s) 219, 255, 433, 530, 815, 887, 1385, 1518
NciI CCSGG 9 cut(s) 872, 873, 909, 927, 945, 1175, 1331, 1817, 1910
NdeII GATC 9 cut(s) 522, 623, 745, 1207, 1218, 1363, 1401, 1569, 1796
NlaIII CATG 8 cut(s) 16, 193, 580, 1756, 1777, 2039, 2069, 2082
NlaIV GGNNCC 8 cut(s) 6, 127, 630, 807, 808, 906, 1947, 2094
NmeAIII GCCGAG 1 cut(s) 696
NmuCI GTSAC 4 cut(s) 730, 1478, 1637, 1828
NruI TCGCGA 1 cut(s) 530
NspI RCATGY 2 cut(s) 2039, 2082
NspV TTCGAA 2 cut(s) 543, 797
PaeI GCATGC 2 cut(s) 2039, 2082
PceI AGGCCT 1 cut(s) 1593
PciSI GCTCTTC 1 cut(s) 1277
PcsI WCGNNNNNNNCGW 4 cut(s) 1002, 1218, 1245, 1556
PctI GAATGC 1 cut(s) 1616
PflFI GACNNNGTC 1 cut(s) 1927
PfoI TCCNGGA 1 cut(s) 925
Ple19I CGATCG 2 cut(s) 1221, 1404
PleI GAGTC 2 cut(s) 948, 1695
PpsI GAGTC 2 cut(s) 948, 1695
Ppu21I YACGTR 1 cut(s) 1273
PpuMI RGGWCCY 2 cut(s) 806, 1945
Psp124BI GAGCTC 3 cut(s) 884, 1245, 1917
Psp5II RGGWCCY 2 cut(s) 806, 1945
Psp6I CCWGG 1 cut(s) 801
PspFI CCCAGC 1 cut(s) 1276
PspGI CCWGG 1 cut(s) 801
PspN4I GGNNCC 8 cut(s) 6, 127, 630, 807, 808, 906, 1947, 2094
PspPI GGNCC 9 cut(s) 174, 401, 473, 598, 806, 905, 1822, 1945, 2092
PspPPI RGGWCCY 2 cut(s) 806, 1945
PsrI GAACNNNNNNTAC 2 cut(s) 1364, 1396
PstNI CAGNNNCTG 1 cut(s) 520
PsuI RGATCY 1 cut(s) 1207
PsyI GACNNNGTC 1 cut(s) 1927
PvuI CGATCG 2 cut(s) 1221, 1404
RruI TCGCGA 1 cut(s) 530
RsaI GTAC 6 cut(s) 1057, 1304, 1445, 1785, 1865, 2052
RsaNI GTAC 6 cut(s) 1056, 1303, 1444, 1784, 1864, 2051
RseI CAYNNNNRTG 1 cut(s) 1422
Rsr2I CGGWCCG 1 cut(s) 473
RsrII CGGWCCG 1 cut(s) 473
SacI GAGCTC 3 cut(s) 884, 1245, 1917
SacII CCGCGG 3 cut(s) 256, 888, 1519
SalI GTCGAC 2 cut(s) 47, 107
SapI GCTCTTC 1 cut(s) 1277
SaqAI TTAA 2 cut(s) 617, 1644
Sau3AI GATC 9 cut(s) 522, 623, 745, 1207, 1218, 1363, 1401, 1569, 1796
Sau96I GGNCC 9 cut(s) 174, 401, 473, 598, 806, 905, 1822, 1945, 2092
SchI GAGTC 2 cut(s) 949, 1695
SduI GDGCHC 5 cut(s) 884, 1245, 1317, 1816, 1917
SfaNI GCATC 4 cut(s) 1037, 1140, 1271, 1426
SfcI CTRYAG 1 cut(s) 2024
Sfr303I CCGCGG 3 cut(s) 256, 888, 1519
SfuI TTCGAA 2 cut(s) 543, 797
SgrBI CCGCGG 3 cut(s) 256, 888, 1519
SinI GGWCC 8 cut(s) 174, 401, 473, 598, 806, 905, 1945, 2092
SmaI CCCGGG 1 cut(s) 873
SmiMI CAYNNNNRTG 1 cut(s) 1422
SmlI CTYRAG 1 cut(s) 1677
SmoI CTYRAG 1 cut(s) 1677
SphI GCATGC 2 cut(s) 2039, 2082
SseBI AGGCCT 1 cut(s) 1593
SspMI CTAG 1 cut(s) 1955
SstI GAGCTC 3 cut(s) 884, 1245, 1917
StuI AGGCCT 1 cut(s) 1593
StyI CCWWGG 2 cut(s) 1575, 2108
TaaI ACNGT 6 cut(s) 386, 736, 1496, 1529, 1637, 1811
TaiI ACGT 5 cut(s) 1238, 1275, 1863, 1931, 2153
TaqII GACCGA 1 cut(s) 1927
TatI WGTACW 2 cut(s) 1302, 1863
TauI GCSGC 9 cut(s) 171, 300, 507, 520, 771, 980, 1362, 1388, 1457
Tru1I TTAA 2 cut(s) 617, 1644
Tru9I TTAA 2 cut(s) 617, 1644
TscAI CASTG 2 cut(s) 1483, 2089
TseFI GTSAC 4 cut(s) 730, 1478, 1637, 1828
TseI GCWGC 4 cut(s) 514, 1077, 1614, 2057
Tsp45I GTSAC 4 cut(s) 730, 1478, 1637, 1828
TspDTI ATGAA 6 cut(s) 206, 1474, 1741, 1790, 2001, 2129
TspGWI ACGGA 2 cut(s) 314, 2110
TspMI CCCGGG 1 cut(s) 871
TspRI CASTG 2 cut(s) 1483, 2089
Tth111I GACNNNGTC 1 cut(s) 1927
VpaK11BI GGWCC 8 cut(s) 174, 401, 473, 598, 806, 905, 1945, 2092
XapI RAATTY 3 cut(s) 552, 564, 898
XceI RCATGY 2 cut(s) 2039, 2082
XmaI CCCGGG 1 cut(s) 871
XmiI GTMKAC 4 cut(s) 48, 108, 288, 2010
XspI CTAG 1 cut(s) 1955
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.