MD13G1126300.v1.1

Possible membrane-associated motif in LPS-induced tumor necrosis factor alpha factor (LITAF), also known as PIG7, and other animal proteins.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0012424
Physical Location & Seq
Reverse (-)
9401018 .. 9402296
1279 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1126300.v1.1.491

Sequence Viewer

Length: 660 bp
ATGTCACGTAGAGGCAAAGGCAATCAATGGAAAAAAACTTGGAAATTTCATGGTTGGTCTCTACCTTCTAGAAGATATAAAAGTCAAGAAATCCCTTTTGATATAAGAGCCCAATGGACATCCTGGTCTCGACACAGCTTTCCCTTGATTCGCTTCTCTTATAAGATCAGCCAGTTCCTATTCCAATCACCAGACCAGACCACTCACAAGATCGCGGGATTTCATCGCACTCAAAACTCACCTGCAGAAGCAGAGATGTCGAAGGTCGAAGAACCGGCGATGGGAGTCCCAGTATACACGGTACAGAATCCGTACCAAGCCGGAATGATCCCGCCAAACGCTGTCTACGGCGATCCCAAGGGCGTCCCTATCCAACAGACCATTTACAGAGACACTCCCGCTCCCTTCAATTGCGCTTACTGTGGCGATACCGGACTAACCAAAGTCAAATCAAAGCCGAGTGCGGCAGCTGTTGTGGCTTGTATGATGCCTATGATGCTTGGATGTTGTTTTCTTCTGCCTTCCTGTGACTGCCTCTGGCATAAGTATCATTACTGCCCAAACTGCCAAGAGAAGGTTGCTGATTTTGAGAAGTCGGACCCTTGTCTTGTTGCGGATCCTTCAAGCTGGACAGAGCGTAGCTATGCACTGCCTGCATGA

Protein Analysis

220

Amino Acids

24.87

Weight (kDa)

8.97

Isoelectric Point (pI)

60.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-LITAF-like PF10601 131 - 195 9.2e-14 LITAF-like zinc ribbon domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012424)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13190 AT5G13190
fragaria_vesca FvH4_4g23080 FvH4_4g23080
malus_domestica MD13G1126300.v1.1 MD16G1126800.v1.1
prunus_persica Prupe.1G219100_v2.0.a1
pyrus_communis pycom13g10980 pycom16g10760
rosa_chinensis RchiOBHm_Chr4g0429351
rosa_multiflora Rmu_sc0003708.1_g000047 Rmu_sc0003904.1_g000002
rosa_roxburghii Rroxscaffold_5G00370960
rosa_rugosa Rorug04G0235200
rosa_samantha Rh4AG291000 Rh4BG296900 Rh4CG312400 Rh4DG293800
rosa_wichuraiana Rw4G025200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 162
AarI CACCTGC 1 cut(s) 250
AasI GACNNNNNNGTC 1 cut(s) 124
Acc36I ACCTGC 1 cut(s) 250
AccBSI CCGCTC 1 cut(s) 401
AccI GTMKAC 2 cut(s) 294, 345
AccII CGCG 1 cut(s) 215
AciI CCGC 5 cut(s) 215, 332, 399, 464, 614
AclWI GGATC 4 cut(s) 322, 347, 611, 624
AcsI RAATTY 1 cut(s) 44
AcyI GRCGYC 1 cut(s) 363
AfaI GTAC 2 cut(s) 303, 314
AfiI CCNNNNNNNGG 2 cut(s) 281, 574
AgsI TTSAA 2 cut(s) 409, 624
AjnI CCWGG 1 cut(s) 122
AluBI AGCT 4 cut(s) 138, 470, 627, 642
AluI AGCT 4 cut(s) 138, 470, 627, 642
Alw26I GTCTC 3 cut(s) 63, 132, 384
AlwI GGATC 4 cut(s) 322, 347, 611, 624
ApeKI GCWGC 1 cut(s) 467
ApoI RAATTY 1 cut(s) 44
AspLEI GCGC 1 cut(s) 416
AspS9I GGNCC 1 cut(s) 598
AsuHPI GGTGA 2 cut(s) 180, 231
AvaII GGWCC 1 cut(s) 598
BamHI GGATCC 1 cut(s) 616
BanII GRGCYC 1 cut(s) 112
BbvI GCAGC 1 cut(s) 479
BccI CCATC 1 cut(s) 274
BceAI ACGGC 1 cut(s) 364
BcgI CGANNNNNNTGC 2 cut(s) 240, 274
BciT130I CCWGG 1 cut(s) 124
BcoDI GTCTC 3 cut(s) 63, 132, 384
BfaI CTAG 1 cut(s) 69
BfmI CTRYAG 1 cut(s) 243
BfuAI ACCTGC 1 cut(s) 250
BisI GCNGC 2 cut(s) 465, 468
BlsI GCNGC 2 cut(s) 466, 469
Bme1390I CCNGG 1 cut(s) 124
Bme18I GGWCC 1 cut(s) 598
BmgT120I GGNCC 1 cut(s) 598
BmiI GGNNCC 2 cut(s) 600, 618
BmrFI CCNGG 1 cut(s) 124
BmrI ACTGGG 1 cut(s) 284
BmsI GCATC 2 cut(s) 477, 486
BmuI ACTGGG 1 cut(s) 284
BoxI GACNNNNGTC 1 cut(s) 603
BsaAI YACGTR 1 cut(s) 8
BsaHI GRCGYC 1 cut(s) 363
BsaI GGTCTC 2 cut(s) 63, 132
BsaJI CCNNGG 1 cut(s) 357
BsaWI WCCGGW 1 cut(s) 431
BsaXI ACNNNNNCTCC 4 cut(s) 276, 306, 385, 415
Bsc4I CCNNNNNNNGG 2 cut(s) 281, 574
Bse118I RCCGGY 1 cut(s) 274
Bse1I ACTGG 2 cut(s) 172, 290
BseBI CCWGG 1 cut(s) 124
BseDI CCNNGG 1 cut(s) 357
BseGI GGATG 2 cut(s) 119, 509
BseLI CCNNNNNNNGG 2 cut(s) 281, 574
BseNI ACTGG 2 cut(s) 172, 290
BseXI GCAGC 1 cut(s) 479
Bsh1236I CGCG 1 cut(s) 215
BsiSI CCGG 3 cut(s) 275, 321, 432
BslFI GGGAC 2 cut(s) 272, 350
BslI CCNNNNNNNGG 2 cut(s) 281, 574
BsmAI GTCTC 3 cut(s) 63, 132, 384
BsmFI GGGAC 2 cut(s) 272, 350
Bso31I GGTCTC 2 cut(s) 63, 132
Bsp1286I GDGCHC 1 cut(s) 112
Bsp143I GATC 5 cut(s) 165, 210, 327, 352, 616
BspACI CCGC 5 cut(s) 215, 332, 399, 464, 614
BspFNI CGCG 1 cut(s) 215
BspLI GGNNCC 2 cut(s) 600, 618
BspMAI CTGCAG 1 cut(s) 247
BspMI ACCTGC 1 cut(s) 250
BspPI GGATC 4 cut(s) 322, 347, 611, 624
BspTNI GGTCTC 2 cut(s) 63, 132
BsrBI CCGCTC 1 cut(s) 401
BsrFI RCCGGY 1 cut(s) 274
BsrI ACTGG 2 cut(s) 172, 290
BssAI RCCGGY 1 cut(s) 274
BssECI CCNNGG 1 cut(s) 357
BssMI GATC 5 cut(s) 165, 210, 327, 352, 616
BssNAI GTATAC 1 cut(s) 295
BssNI GRCGYC 1 cut(s) 363
BssT1I CCWWGG 1 cut(s) 357
Bst1107I GTATAC 1 cut(s) 295
Bst2UI CCWGG 1 cut(s) 124
Bst4CI ACNGT 2 cut(s) 301, 422
BstACI GRCGYC 1 cut(s) 363
BstAPI GCANNNNNTGC 1 cut(s) 653
BstBAI YACGTR 1 cut(s) 8
BstC8I GCNNGC 1 cut(s) 654
BstF5I GGATG 2 cut(s) 119, 509
BstFNI CGCG 1 cut(s) 215
BstHHI GCGC 1 cut(s) 416
BstKTI GATC 5 cut(s) 168, 213, 330, 355, 619
BstMAI GTCTC 3 cut(s) 63, 132, 384
BstMBI GATC 5 cut(s) 165, 210, 327, 352, 616
BstMWI GCNNNNNNNGC 4 cut(s) 476, 496, 564, 653
BstNI CCWGG 1 cut(s) 124
BstPAI GACNNNNGTC 1 cut(s) 603
BstSCI CCNGG 1 cut(s) 122
BstSFI CTRYAG 1 cut(s) 243
BstUI CGCG 1 cut(s) 215
BstV1I GCAGC 1 cut(s) 479
BstX2I RGATCY 1 cut(s) 616
BstYI RGATCY 1 cut(s) 616
BstZ17I GTATAC 1 cut(s) 295
BtgZI GCGATG 2 cut(s) 209, 293
BtsCI GGATG 2 cut(s) 119, 509
BtsI GCAGTG 1 cut(s) 647
BtsIMutI CAGTG 1 cut(s) 647
BveI ACCTGC 1 cut(s) 250
Cac8I GCNNGC 1 cut(s) 654
CfoI GCGC 1 cut(s) 416
Cfr10I RCCGGY 1 cut(s) 274
Cfr13I GGNCC 1 cut(s) 598
CseI GACGC 1 cut(s) 352
Csp6I GTAC 2 cut(s) 302, 313
CviAII CATG 2 cut(s) 50, 657
CviJI RGCY 9 cut(s) 110, 138, 171, 320, 457, 470, 479, 627, 642
CviKI_1 RGCY 9 cut(s) 110, 138, 171, 320, 457, 470, 479, 627, 642
CviQI GTAC 2 cut(s) 302, 313
DpnI GATC 5 cut(s) 167, 212, 329, 354, 618
DpnII GATC 5 cut(s) 165, 210, 327, 352, 616
DrdI GACNNNNNNGTC 1 cut(s) 124
DseDI GACNNNNNNGTC 1 cut(s) 124
Eco130I CCWWGG 1 cut(s) 357
Eco24I GRGCYC 1 cut(s) 112
Eco31I GGTCTC 2 cut(s) 63, 132
Eco47I GGWCC 1 cut(s) 598
EcoRII CCWGG 1 cut(s) 122
EcoT14I CCWWGG 1 cut(s) 357
EcoT38I GRGCYC 1 cut(s) 112
ErhI CCWWGG 1 cut(s) 357
FaeI CATG 2 cut(s) 53, 660
FaqI GGGAC 2 cut(s) 272, 350
FatI CATG 2 cut(s) 49, 656
FauI CCCGC 3 cut(s) 208, 339, 406
FblI GTMKAC 2 cut(s) 294, 345
Fnu4HI GCNGC 2 cut(s) 465, 468
FokI GGATG 2 cut(s) 106, 516
FriOI GRGCYC 1 cut(s) 112
Fsp4HI GCNGC 2 cut(s) 465, 468
FspBI CTAG 1 cut(s) 69
GlaI GCGC 1 cut(s) 415
GluI GCNGC 2 cut(s) 465, 468
HapII CCGG 3 cut(s) 275, 321, 432
HgaI GACGC 1 cut(s) 352
HhaI GCGC 1 cut(s) 416
Hin1I GRCGYC 1 cut(s) 363
Hin1II CATG 2 cut(s) 53, 660
Hin6I GCGC 1 cut(s) 414
HinP1I GCGC 1 cut(s) 414
HinfI GANTC 3 cut(s) 148, 285, 307
HpaII CCGG 3 cut(s) 275, 321, 432
HphI GGTGA 2 cut(s) 180, 231
Hpy166II GTNNAC 2 cut(s) 295, 346
Hpy188I TCNGA 1 cut(s) 598
Hpy188III TCNNGA 3 cut(s) 69, 86, 129
Hpy8I GTNNAC 2 cut(s) 295, 346
HpyAV CCTTC 6 cut(s) 75, 256, 415, 531, 568, 630
HpyCH4III ACNGT 2 cut(s) 301, 422
HpyCH4IV ACGT 1 cut(s) 7
HpyCH4V TGCA 3 cut(s) 245, 647, 656
HpyF10VI GCNNNNNNNGC 4 cut(s) 476, 496, 564, 653
HpySE526I ACGT 1 cut(s) 7
Hsp92I GRCGYC 1 cut(s) 363
Hsp92II CATG 2 cut(s) 53, 660
HspAI GCGC 1 cut(s) 414
Kzo9I GATC 5 cut(s) 165, 210, 327, 352, 616
LmnI GCTCC 1 cut(s) 406
Lsp1109I GCAGC 1 cut(s) 479
LweI GCATC 2 cut(s) 477, 486
MaeI CTAG 1 cut(s) 69
MaeII ACGT 1 cut(s) 7
MaeIII GTNAC 2 cut(s) 3, 527
MalI GATC 5 cut(s) 167, 212, 329, 354, 618
MbiI CCGCTC 1 cut(s) 401
MboI GATC 5 cut(s) 165, 210, 327, 352, 616
MboII GAAGA 3 cut(s) 84, 281, 506
MfeI CAATTG 1 cut(s) 409
MflI RGATCY 1 cut(s) 616
MhlI GDGCHC 1 cut(s) 112
MluCI AATT 2 cut(s) 44, 409
MlyI GAGTC 1 cut(s) 294
MmeI TCCRAC 2 cut(s) 397, 576
MnlI CCTC 2 cut(s) 5, 545
MspA1I CMGCKG 1 cut(s) 470
MspI CCGG 3 cut(s) 275, 321, 432
MspR9I CCNGG 1 cut(s) 124
MunI CAATTG 1 cut(s) 409
MvaI CCWGG 1 cut(s) 124
MvnI CGCG 1 cut(s) 215
MwoI GCNNNNNNNGC 4 cut(s) 476, 496, 564, 653
NdeII GATC 5 cut(s) 165, 210, 327, 352, 616
NlaIII CATG 2 cut(s) 53, 660
NlaIV GGNNCC 2 cut(s) 600, 618
NmeAIII GCCGAG 1 cut(s) 483
NmuCI GTSAC 2 cut(s) 3, 527
PaqCI CACCTGC 1 cut(s) 250
PfeI GAWTC 2 cut(s) 148, 307
PkrI GCNGC 2 cut(s) 466, 469
PleI GAGTC 1 cut(s) 293
PpsI GAGTC 1 cut(s) 293
Ppu21I YACGTR 1 cut(s) 8
PshAI GACNNNNGTC 1 cut(s) 603
PsiI TTATAA 1 cut(s) 162
Psp6I CCWGG 1 cut(s) 122
PspGI CCWGG 1 cut(s) 122
PspN4I GGNNCC 2 cut(s) 600, 618
PspPI GGNCC 1 cut(s) 598
PstI CTGCAG 1 cut(s) 247
PsuI RGATCY 1 cut(s) 616
PvuII CAGCTG 1 cut(s) 470
RsaI GTAC 2 cut(s) 303, 314
RsaNI GTAC 2 cut(s) 302, 313
SatI GCNGC 2 cut(s) 465, 468
Sau3AI GATC 5 cut(s) 165, 210, 327, 352, 616
Sau96I GGNCC 1 cut(s) 598
SchI GAGTC 1 cut(s) 294
ScrFI CCNGG 1 cut(s) 124
SduI GDGCHC 1 cut(s) 112
SetI ASST 9 cut(s) 10, 67, 140, 244, 267, 472, 579, 629, 644
SfaNI GCATC 2 cut(s) 477, 486
SfcI CTRYAG 1 cut(s) 243
SinI GGWCC 1 cut(s) 598
Sse9I AATT 2 cut(s) 44, 409
SsiI CCGC 5 cut(s) 215, 332, 399, 464, 614
SspMI CTAG 1 cut(s) 69
StyD4I CCNGG 1 cut(s) 122
StyI CCWWGG 1 cut(s) 357
TaaI ACNGT 2 cut(s) 301, 422
TaiI ACGT 1 cut(s) 10
TaqI TCGA 3 cut(s) 130, 260, 267
TasI AATT 2 cut(s) 44, 409
TauI GCSGC 1 cut(s) 467
TfiI GAWTC 2 cut(s) 148, 307
TscAI CASTG 1 cut(s) 654
TseFI GTSAC 2 cut(s) 3, 527
TseI GCWGC 1 cut(s) 467
Tsp45I GTSAC 2 cut(s) 3, 527
TspDTI ATGAA 2 cut(s) 38, 212
TspGWI ACGGA 1 cut(s) 300
TspRI CASTG 1 cut(s) 654
VpaK11BI GGWCC 1 cut(s) 598
XapI RAATTY 1 cut(s) 44
XbaI TCTAGA 1 cut(s) 68
XmiI GTMKAC 2 cut(s) 294, 345
XspI CTAG 1 cut(s) 69
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.