Rh4AG291000

Possible membrane-associated motif in LPS-induced tumor necrosis factor alpha factor (LITAF), also known as PIG7, and other animal proteins.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0012424
Physical Location & Seq
Forward (+)
60457122 .. 60460492
3371 bp
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UTR
Exon/CDS
Intron
Rh4AG291000.1

Sequence Viewer

Length: 441 bp
ATGCAAGGATACCCTCCACCACCGCATCAGGCGGAGATGTCGAAGATGCCGCCGCAGCAGCAACCGCCGGTGATGGGAGAGCCGGTGATGGGAGTCCCCTACTACGTCCACGAGAATCCCTACCAGGCCGGCATGGTTCCACCCAACGCCATTTACGGCGACCCAAAAGGAGTTCCGCTTCAACAAACCATCTACAGAGACACACCCGCTCCTTTCAACTGCCTTTACTGCGGCGACACCGGACTCACCCAAGTCAGATCAAAGCCGAGTCTGGCATCTGTTGTGGGGTGTTTGATGTGTTGTGGTTGTGGGTTCTGCTTTCTTTTGCGTTCCATGGACTGCCTCTGGCATAAGTACCATTATTGCTCACACTGCCACCAAAAGGTAATGTGGCTTATGACTTCTCTCTCAAATTTCTATGATTTTTCGGGTAGAAATTGA

Protein Analysis

146

Amino Acids

16.43

Weight (kDa)

7.54

Isoelectric Point (pI)

66.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-LITAF-like PF10601 67 - 129 2.9e-11 LITAF-like zinc ribbon domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0012424)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13190 AT5G13190
fragaria_vesca FvH4_4g23080 FvH4_4g23080
malus_domestica MD13G1126300.v1.1 MD16G1126800.v1.1
prunus_persica Prupe.1G219100_v2.0.a1
pyrus_communis pycom13g10980 pycom16g10760
rosa_chinensis RchiOBHm_Chr4g0429351
rosa_multiflora Rmu_sc0003708.1_g000047 Rmu_sc0003904.1_g000002
rosa_roxburghii Rroxscaffold_5G00370960
rosa_rugosa Rorug04G0235200
rosa_samantha Rh4AG291000 Rh4BG296900 Rh4CG312400 Rh4DG293800
rosa_wichuraiana Rw4G025200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 209
AciI CCGC 8 cut(s) 23, 32, 50, 53, 65, 176, 207, 231
AcsI RAATTY 1 cut(s) 412
AfaI GTAC 1 cut(s) 356
AfiI CCNNNNNNNGG 3 cut(s) 74, 89, 382
AgsI TTSAA 2 cut(s) 182, 217
AjnI CCWGG 1 cut(s) 123
Alw26I GTCTC 1 cut(s) 192
AoxI GGCC 1 cut(s) 126
ApeKI GCWGC 2 cut(s) 55, 58
ApoI RAATTY 1 cut(s) 412
AsuHPI GGTGA 3 cut(s) 82, 97, 238
BauI CACGAG 1 cut(s) 110
BbvI GCAGC 2 cut(s) 67, 70
BccI CCATC 3 cut(s) 67, 82, 197
BceAI ACGGC 1 cut(s) 172
BciT130I CCWGG 1 cut(s) 125
BcoDI GTCTC 1 cut(s) 192
BfmI CTRYAG 1 cut(s) 193
BisI GCNGC 5 cut(s) 50, 53, 56, 59, 232
BlsI GCNGC 5 cut(s) 51, 54, 57, 60, 233
Bme1390I CCNGG 1 cut(s) 125
BmiI GGNNCC 1 cut(s) 138
BmrFI CCNGG 1 cut(s) 125
BmsI GCATC 3 cut(s) 34, 36, 284
BsaJI CCNNGG 1 cut(s) 333
BsaWI WCCGGW 1 cut(s) 239
BsaXI ACNNNNNCTCC 4 cut(s) 69, 99, 193, 223
Bsc4I CCNNNNNNNGG 3 cut(s) 74, 89, 382
Bse118I RCCGGY 3 cut(s) 67, 82, 128
BseBI CCWGG 1 cut(s) 125
BseDI CCNNGG 1 cut(s) 333
BseLI CCNNNNNNNGG 3 cut(s) 74, 89, 382
BseXI GCAGC 2 cut(s) 67, 70
BshFI GGCC 1 cut(s) 128
BsiSI CCGG 4 cut(s) 68, 83, 129, 240
BslFI GGGAC 1 cut(s) 80
BslI CCNNNNNNNGG 3 cut(s) 74, 89, 382
BsmAI GTCTC 1 cut(s) 192
BsmFI GGGAC 1 cut(s) 80
BsnI GGCC 1 cut(s) 128
Bsp143I GATC 1 cut(s) 257
Bsp19I CCATGG 1 cut(s) 333
BspACI CCGC 8 cut(s) 23, 32, 50, 53, 65, 176, 207, 231
BspANI GGCC 1 cut(s) 128
BspLI GGNNCC 1 cut(s) 138
BsrBI CCGCTC 1 cut(s) 209
BsrFI RCCGGY 3 cut(s) 67, 82, 128
BssAI RCCGGY 3 cut(s) 67, 82, 128
BssECI CCNNGG 1 cut(s) 333
BssMI GATC 1 cut(s) 257
BssSI CACGAG 1 cut(s) 110
BssT1I CCWWGG 1 cut(s) 333
Bst2BI CACGAG 1 cut(s) 110
Bst2UI CCWGG 1 cut(s) 125
BstC8I GCNNGC 1 cut(s) 130
BstDSI CCRYGG 1 cut(s) 333
BstKTI GATC 1 cut(s) 260
BstMAI GTCTC 1 cut(s) 192
BstMBI GATC 1 cut(s) 257
BstMWI GCNNNNNNNGC 5 cut(s) 55, 58, 64, 228, 372
BstNI CCWGG 1 cut(s) 125
BstSCI CCNGG 1 cut(s) 123
BstSFI CTRYAG 1 cut(s) 193
BstV1I GCAGC 2 cut(s) 67, 70
BsuRI GGCC 1 cut(s) 128
BtgI CCRYGG 1 cut(s) 333
BtsI GCAGTG 1 cut(s) 370
BtsIMutI CAGTG 1 cut(s) 370
Cac8I GCNNGC 1 cut(s) 130
Cfr10I RCCGGY 3 cut(s) 67, 82, 128
Csp6I GTAC 1 cut(s) 355
CviAII CATG 2 cut(s) 133, 334
CviJI RGCY 4 cut(s) 82, 128, 265, 394
CviKI_1 RGCY 4 cut(s) 82, 128, 265, 394
CviQI GTAC 1 cut(s) 355
DpnI GATC 1 cut(s) 259
DpnII GATC 1 cut(s) 257
EciI GGCGGA 1 cut(s) 47
Eco130I CCWWGG 1 cut(s) 333
EcoRII CCWGG 1 cut(s) 123
EcoT14I CCWWGG 1 cut(s) 333
ErhI CCWWGG 1 cut(s) 333
FaeI CATG 2 cut(s) 136, 337
FaiI YATR 5 cut(s) 134, 335, 351, 398, 420
FaqI GGGAC 1 cut(s) 80
FatI CATG 2 cut(s) 132, 333
FauI CCCGC 1 cut(s) 214
Fnu4HI GCNGC 5 cut(s) 50, 53, 56, 59, 232
Fsp4HI GCNGC 5 cut(s) 50, 53, 56, 59, 232
GluI GCNGC 5 cut(s) 50, 53, 56, 59, 232
HaeIII GGCC 1 cut(s) 128
HapII CCGG 4 cut(s) 68, 83, 129, 240
Hin1II CATG 2 cut(s) 136, 337
HinfI GANTC 4 cut(s) 93, 115, 243, 268
HpaII CCGG 4 cut(s) 68, 83, 129, 240
HphI GGTGA 3 cut(s) 82, 97, 238
Hpy166II GTNNAC 1 cut(s) 109
Hpy188I TCNGA 1 cut(s) 257
Hpy8I GTNNAC 1 cut(s) 109
HpyCH4IV ACGT 1 cut(s) 105
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 5 cut(s) 55, 58, 64, 228, 372
HpySE526I ACGT 1 cut(s) 105
Hsp92II CATG 2 cut(s) 136, 337
KroI GCCGGC 1 cut(s) 128
KroNI GCCGGC 1 cut(s) 130
Kzo9I GATC 1 cut(s) 257
LmnI GCTCC 1 cut(s) 214
LpnPI CCDG 9 cut(s) 14, 81, 96, 110, 137, 142, 253, 257, 331
Lsp1109I GCAGC 2 cut(s) 67, 70
LweI GCATC 3 cut(s) 34, 36, 284
MaeII ACGT 1 cut(s) 105
MalI GATC 1 cut(s) 259
MbiI CCGCTC 1 cut(s) 209
MboI GATC 1 cut(s) 257
MboII GAAGA 1 cut(s) 55
MluCI AATT 2 cut(s) 412, 436
MlyI GAGTC 3 cut(s) 102, 237, 277
MnlI CCTC 2 cut(s) 24, 353
MroNI GCCGGC 1 cut(s) 128
MspI CCGG 4 cut(s) 68, 83, 129, 240
MspR9I CCNGG 1 cut(s) 125
MvaI CCWGG 1 cut(s) 125
MwoI GCNNNNNNNGC 5 cut(s) 55, 58, 64, 228, 372
NaeI GCCGGC 1 cut(s) 130
NcoI CCATGG 1 cut(s) 333
NdeII GATC 1 cut(s) 257
NgoMIV GCCGGC 1 cut(s) 128
NlaIII CATG 2 cut(s) 136, 337
NlaIV GGNNCC 1 cut(s) 138
NmeAIII GCCGAG 1 cut(s) 291
PdiI GCCGGC 1 cut(s) 130
PfeI GAWTC 1 cut(s) 115
PkrI GCNGC 5 cut(s) 51, 54, 57, 60, 233
PleI GAGTC 3 cut(s) 101, 237, 276
PpsI GAGTC 3 cut(s) 101, 237, 276
Psp6I CCWGG 1 cut(s) 123
PspGI CCWGG 1 cut(s) 123
PspN4I GGNNCC 1 cut(s) 138
RsaI GTAC 1 cut(s) 356
RsaNI GTAC 1 cut(s) 355
SatI GCNGC 5 cut(s) 50, 53, 56, 59, 232
Sau3AI GATC 1 cut(s) 257
SchI GAGTC 3 cut(s) 102, 237, 277
ScrFI CCNGG 1 cut(s) 125
SetI ASST 2 cut(s) 108, 387
SfaNI GCATC 3 cut(s) 34, 36, 284
SfcI CTRYAG 1 cut(s) 193
SgrAI CRCCGGYG 1 cut(s) 67
Sse9I AATT 2 cut(s) 412, 436
SsiI CCGC 8 cut(s) 23, 32, 50, 53, 65, 176, 207, 231
StyD4I CCNGG 1 cut(s) 123
StyI CCWWGG 1 cut(s) 333
TaiI ACGT 1 cut(s) 108
TaqI TCGA 1 cut(s) 41
TasI AATT 2 cut(s) 412, 436
TauI GCSGC 3 cut(s) 52, 55, 234
TfiI GAWTC 1 cut(s) 115
TscAI CASTG 1 cut(s) 377
TseI GCWGC 2 cut(s) 55, 58
TspRI CASTG 1 cut(s) 377
XapI RAATTY 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.