MD16G1126800.v1.1

Possible membrane-associated motif in LPS-induced tumor necrosis factor alpha factor (LITAF), also known as PIG7, and other animal proteins.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0012424
Physical Location & Seq
Reverse (-)
9227058 .. 9228642
1585 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1126800.v1.1.491

Sequence Viewer

Length: 405 bp
ATGTCGAAGGTGGACGAACCGGTGATGGGAGTTCCAGTCTACACGGTGCAGAATCCGTACCAGGCCGGAATGATCCCGCCAAACGCCGTCTACGGCGACCCCAAGGGCGTCCCTCTCCACCAGACCTTTTACAGAGACACTCCCGCTCCCTTCAACTGCGCTTACTGTGGCGATACCGGACTCACCCAAATCAGATCAAAGCCGAGTATGGCATCTGTGGTGGCTTGTATGATGCCTATGATGCTTGGATGTTGCTTTCTTATGCCTTCCTGTGACTGCCTCTGGCATAAGTATCATTACTGTCCAAGTTGCCATGAGAAGGTTGCTGATTTTGAGAAGTCGGATATTTGCATTGTTGCGGATCCTCCACACTGGACAGAGCACAGCTTTGCACTGCCTGCATGA

Protein Analysis

135

Amino Acids

14.87

Weight (kDa)

5.77

Isoelectric Point (pI)

60.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-LITAF-like PF10601 46 - 110 3.7e-13 LITAF-like zinc ribbon domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012424)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G13190 AT5G13190
fragaria_vesca FvH4_4g23080 FvH4_4g23080
malus_domestica MD13G1126300.v1.1 MD16G1126800.v1.1
prunus_persica Prupe.1G219100_v2.0.a1
pyrus_communis pycom13g10980 pycom16g10760
rosa_chinensis RchiOBHm_Chr4g0429351
rosa_multiflora Rmu_sc0003708.1_g000047 Rmu_sc0003904.1_g000002
rosa_roxburghii Rroxscaffold_5G00370960
rosa_rugosa Rorug04G0235200
rosa_samantha Rh4AG291000 Rh4BG296900 Rh4CG312400 Rh4DG293800
rosa_wichuraiana Rw4G025200

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 146
AccI GTMKAC 2 cut(s) 39, 90
AciI CCGC 3 cut(s) 77, 144, 359
AclWI GGATC 3 cut(s) 67, 356, 369
AcyI GRCGYC 1 cut(s) 108
AfaI GTAC 1 cut(s) 59
AfiI CCNNNNNNNGG 2 cut(s) 26, 319
AgeI ACCGGT 1 cut(s) 19
AgsI TTSAA 1 cut(s) 154
AjnI CCWGG 1 cut(s) 60
AluBI AGCT 1 cut(s) 387
AluI AGCT 1 cut(s) 387
Alw21I GWGCWC 1 cut(s) 384
Alw26I GTCTC 1 cut(s) 129
AlwI GGATC 3 cut(s) 67, 356, 369
AoxI GGCC 1 cut(s) 63
AsiGI ACCGGT 1 cut(s) 19
AspLEI GCGC 1 cut(s) 161
AsuHPI GGTGA 2 cut(s) 34, 175
BamHI GGATCC 1 cut(s) 361
Bbv12I GWGCWC 1 cut(s) 384
BccI CCATC 1 cut(s) 19
BceAI ACGGC 2 cut(s) 71, 109
BciT130I CCWGG 1 cut(s) 62
BcoDI GTCTC 1 cut(s) 129
Bme1390I CCNGG 1 cut(s) 62
BmiI GGNNCC 1 cut(s) 363
BmrFI CCNGG 1 cut(s) 62
BmsI GCATC 3 cut(s) 221, 222, 231
BsaHI GRCGYC 1 cut(s) 108
BsaJI CCNNGG 1 cut(s) 102
BsaWI WCCGGW 2 cut(s) 19, 176
BsaXI ACNNNNNCTCC 4 cut(s) 21, 51, 130, 160
Bsc4I CCNNNNNNNGG 2 cut(s) 26, 319
Bse118I RCCGGY 1 cut(s) 19
Bse1I ACTGG 2 cut(s) 35, 377
BseBI CCWGG 1 cut(s) 62
BseDI CCNNGG 1 cut(s) 102
BseGI GGATG 1 cut(s) 254
BseLI CCNNNNNNNGG 2 cut(s) 26, 319
BseNI ACTGG 2 cut(s) 35, 377
BsgI GTGCAG 1 cut(s) 68
BshFI GGCC 1 cut(s) 65
BshTI ACCGGT 1 cut(s) 19
BsiHKAI GWGCWC 1 cut(s) 384
BsiSI CCGG 3 cut(s) 20, 66, 177
BslFI GGGAC 1 cut(s) 95
BslI CCNNNNNNNGG 2 cut(s) 26, 319
BsmAI GTCTC 1 cut(s) 129
BsmFI GGGAC 1 cut(s) 95
BsnI GGCC 1 cut(s) 65
Bsp1286I GDGCHC 1 cut(s) 384
Bsp143I GATC 3 cut(s) 72, 194, 361
BspACI CCGC 3 cut(s) 77, 144, 359
BspANI GGCC 1 cut(s) 65
BspLI GGNNCC 1 cut(s) 363
BspPI GGATC 3 cut(s) 67, 356, 369
BsrBI CCGCTC 1 cut(s) 146
BsrFI RCCGGY 1 cut(s) 19
BsrI ACTGG 2 cut(s) 35, 377
BssAI RCCGGY 1 cut(s) 19
BssECI CCNNGG 1 cut(s) 102
BssMI GATC 3 cut(s) 72, 194, 361
BssNI GRCGYC 1 cut(s) 108
BssT1I CCWWGG 1 cut(s) 102
Bst2UI CCWGG 1 cut(s) 62
Bst4CI ACNGT 3 cut(s) 46, 167, 302
BstACI GRCGYC 1 cut(s) 108
BstAPI GCANNNNNTGC 1 cut(s) 398
BstC8I GCNNGC 1 cut(s) 399
BstF5I GGATG 1 cut(s) 254
BstHHI GCGC 1 cut(s) 161
BstKTI GATC 3 cut(s) 75, 197, 364
BstMAI GTCTC 1 cut(s) 129
BstMBI GATC 3 cut(s) 72, 194, 361
BstMWI GCNNNNNNNGC 2 cut(s) 241, 398
BstNI CCWGG 1 cut(s) 62
BstSCI CCNGG 1 cut(s) 60
BstX2I RGATCY 1 cut(s) 361
BstYI RGATCY 1 cut(s) 361
BsuRI GGCC 1 cut(s) 65
BtsCI GGATG 1 cut(s) 254
BtsI GCAGTG 1 cut(s) 392
BtsIMutI CAGTG 2 cut(s) 370, 392
Cac8I GCNNGC 1 cut(s) 399
CfoI GCGC 1 cut(s) 161
Cfr10I RCCGGY 1 cut(s) 19
CseI GACGC 1 cut(s) 97
Csp6I GTAC 1 cut(s) 58
CspAI ACCGGT 1 cut(s) 19
CviAII CATG 2 cut(s) 314, 402
CviJI RGCY 4 cut(s) 65, 202, 224, 387
CviKI_1 RGCY 4 cut(s) 65, 202, 224, 387
CviQI GTAC 1 cut(s) 58
DpnI GATC 3 cut(s) 74, 196, 363
DpnII GATC 3 cut(s) 72, 194, 361
Eco130I CCWWGG 1 cut(s) 102
EcoRII CCWGG 1 cut(s) 60
EcoT14I CCWWGG 1 cut(s) 102
ErhI CCWWGG 1 cut(s) 102
FaeI CATG 2 cut(s) 317, 405
FaiI YATR 7 cut(s) 209, 230, 239, 263, 288, 315, 403
FaqI GGGAC 1 cut(s) 95
FatI CATG 2 cut(s) 313, 401
FauI CCCGC 2 cut(s) 84, 151
FblI GTMKAC 2 cut(s) 39, 90
FokI GGATG 1 cut(s) 261
GlaI GCGC 1 cut(s) 160
HaeIII GGCC 1 cut(s) 65
HapII CCGG 3 cut(s) 20, 66, 177
HgaI GACGC 1 cut(s) 97
HhaI GCGC 1 cut(s) 161
Hin1I GRCGYC 1 cut(s) 108
Hin1II CATG 2 cut(s) 317, 405
Hin6I GCGC 1 cut(s) 159
HinP1I GCGC 1 cut(s) 159
HinfI GANTC 2 cut(s) 52, 180
HpaII CCGG 3 cut(s) 20, 66, 177
HphI GGTGA 2 cut(s) 34, 175
Hpy166II GTNNAC 3 cut(s) 13, 40, 91
Hpy188I TCNGA 2 cut(s) 194, 343
Hpy8I GTNNAC 3 cut(s) 13, 40, 91
HpyAV CCTTC 3 cut(s) 160, 276, 313
HpyCH4III ACNGT 3 cut(s) 46, 167, 302
HpyCH4V TGCA 4 cut(s) 49, 351, 392, 401
HpyF10VI GCNNNNNNNGC 2 cut(s) 241, 398
Hsp92I GRCGYC 1 cut(s) 108
Hsp92II CATG 2 cut(s) 317, 405
HspAI GCGC 1 cut(s) 159
Kzo9I GATC 3 cut(s) 72, 194, 361
LmnI GCTCC 1 cut(s) 151
LweI GCATC 3 cut(s) 221, 222, 231
MaeIII GTNAC 1 cut(s) 272
MalI GATC 3 cut(s) 74, 196, 363
MbiI CCGCTC 1 cut(s) 146
MboI GATC 3 cut(s) 72, 194, 361
MflI RGATCY 1 cut(s) 361
MhlI GDGCHC 1 cut(s) 384
MlyI GAGTC 1 cut(s) 174
MmeI TCCRAC 1 cut(s) 321
MnlI CCTC 3 cut(s) 123, 290, 375
MspI CCGG 3 cut(s) 20, 66, 177
MspR9I CCNGG 1 cut(s) 62
MvaI CCWGG 1 cut(s) 62
MwoI GCNNNNNNNGC 2 cut(s) 241, 398
NdeII GATC 3 cut(s) 72, 194, 361
NlaIII CATG 2 cut(s) 317, 405
NlaIV GGNNCC 1 cut(s) 363
NmeAIII GCCGAG 1 cut(s) 228
NmuCI GTSAC 1 cut(s) 272
PfeI GAWTC 1 cut(s) 52
PinAI ACCGGT 1 cut(s) 19
PleI GAGTC 1 cut(s) 174
PpsI GAGTC 1 cut(s) 174
Psp6I CCWGG 1 cut(s) 60
PspGI CCWGG 1 cut(s) 60
PspN4I GGNNCC 1 cut(s) 363
PsuI RGATCY 1 cut(s) 361
RsaI GTAC 1 cut(s) 59
RsaNI GTAC 1 cut(s) 58
Sau3AI GATC 3 cut(s) 72, 194, 361
SchI GAGTC 1 cut(s) 174
ScrFI CCNGG 1 cut(s) 62
SduI GDGCHC 1 cut(s) 384
SetI ASST 4 cut(s) 12, 128, 324, 389
SfaNI GCATC 3 cut(s) 221, 222, 231
SsiI CCGC 3 cut(s) 77, 144, 359
StyD4I CCNGG 1 cut(s) 60
StyI CCWWGG 1 cut(s) 102
TaaI ACNGT 3 cut(s) 46, 167, 302
TaqI TCGA 1 cut(s) 5
TfiI GAWTC 1 cut(s) 52
TscAI CASTG 2 cut(s) 377, 399
TseFI GTSAC 1 cut(s) 272
Tsp45I GTSAC 1 cut(s) 272
TspGWI ACGGA 1 cut(s) 45
TspRI CASTG 2 cut(s) 377, 399
XmiI GTMKAC 2 cut(s) 39, 90
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.