MD13G1130300.v1.1

Protein MEI2-like 4 isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0001846
Physical Location & Seq
Forward (+)
9890491 .. 9897642
7152 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1130300.v1.1.491

Sequence Viewer

Length: 2937 bp
ATGGACCTCAAGGGTTTGTCCTCCTCGTCGTTCTTCTCCGATGAAAGGCAGGGTGGGTTTTGGAAGTCAGATCACTTGCCTGATAATCATGCCAGCAAGAAGTCACTTGCTTCATCATCTCTGGAGACGTGTCAGACAGTGAATTCTCTGGACCATCCCGAATTTTTCATAATGCAAGACCAGAAAGTGCATCCCGGCTTCAATAAACAGGCAGTGGGAGCAGAGAGAGCCTTAAGTCATTCCTTGAATTTGTCGAGAACCATGAACCATGATTCAGGAGCAAGATCTAATGTAAATGTTGAGACAGCATCTTATTTTGGAGAAGGTGGTAAAGTCAATATGATGGGAGCTCAGTATGAGAGTAGCCTTTTTTCAAGTTCATTGTCGGAGCTATTTAGTAGGAAGTTAAGATTATCGTCGAACAATACACTGTATGGCCATTCTGTTGATACTGTTGCCCCCCACTATGATGAAGAGGAAGCTTTTGAATCCCTTGAAGAAATTGAGGCCCAAACCATCGGAAATCTTCTCCCAAATGATGACGAGTTGCTTTCTGGAGTTACCGATGGGCTTGACTATTCAGTCCAACTGAGTGCTAGGGATAATATGGAGGAGTTGGACCTTTTTAGCAGTGTTGGAGGGATGGATTTGGGAGATGACGGTTTGGCAACTGGATTAAGGGACTCTGTGTATGCTGTAGAAGTTTCTAATGGTCTGTCGTGCAATGGTTCAGTAGTGGGAGAACACCCTTATGGTGAACACCCTTCCCGGACTTTGTTTGTGAGAAATATAAACAGTAACATTGAAGATTCTGAGTTGAGAACCCTGTTTGAGCAATATGGAGATATTCGCACTCTTTATACAGCCTGCAAGCATCGCGGTTTTGTTATGATATCCTATTATGACATTAGAGCAGCTCGTAATGCGATGAAATCACTGCAGAATAAACCATTGAGGCGCCGCAAGCTTGACATACATTACTCAATTCCAAAGGACAACCCTTCTGAGAAAGATGTTAATCAGGGAACTCTTGTTGTATTCAACCTGGATTCTTCTGTTTCAAATGATGAATTACGCCAGGTTTTTGGTGTCTATGGAGAAATCAAAGAGATCCGTGAAACCCCAAATAGAAGTCATAACAAATTTATTGAATTTTATGACATTAGAGCTGCAGACGCTGCTCTTAATGCCTTAAACAAGAGTGATATTGCCGGGAAGCAAATTAAGCTTGAGCCAAGCCGTCCTGGAGGTGCAAGACGGAGCTTGGAGCTACAGTTATCTTCAGATTTTTTAGAGCAAGATGAATGTGGCCTATATCTGCAGCAGACTAGCCCTTCTGACTCTATCACTGGATTTTCTGGACCAGTTCCACATGGATCAGTTACGTCCAGTTGCACAGATAATGGAACTATCATGGCTGTACACGCTGCAGCACAAGCTGCGTCCCTTGAAAATGCATTCCACTATGGGGTCTCTTCTAGTGTTCCTAACAGCTTACCTTCTGTTCTGAGAGCTGAATCTGTTGGGAATCAGTCTGGCTTTGTTGAGTCTGTCCATTCAGCAGGCTCGCTGAAATTTGACATCCATGGCTTGCCAGCTTCCCATCCTCATTCACTTCCAGAGTATCATGATGGTTTAACTAATTCAGTCAACTGCAGTTCACCGGGCAATGTATCTATCAATGCCAGACCAACAGAAAGAATTGATAACAGGCACTTCCCCAGAGTAAGCTCCAATGGGCACTCATATGAACTAAATGAAGGTGTTTTTGGCTCTGCCGGCAATGTTAACTGCCCTATTCCTGGACATCATTACGCATGGAACAACTCCTATCACCCTCAGCCTCCTGGAATGATATGGCCAAACTCACCATCATTTGTCAATGGACTTTCTTCAGCTCATCCCTTTTCTGCAGTCCATCCCCCAACGCGGGTTCATGGACTTCCTAGAGCACCATCTCATATGTTAAACCCAGGTTTGCCCATGCATAACCATCATGTGGGGTCAGCACCAGTGGTCAATCCTTCTCTGTGGGACAGACGACAATCATATGGAGGGGAATCCCCCGAGGCATCTGGGTTTCATCCAGGCTCTCTTGGAAATATGAGAATCTCAAATAACTCACCACATTCAATGGAATTTGTTTCTCATAACATGTTTCCTCAGGTTGGTGGAAACAGCATGGACCTGCCAATTGCTCACAAAAATGCAGGACTGCAGCAAGCCCATCATCAGAGGTGCATGATGTTTCCTGGAAGAGGCCAGATGATTCCCATCATGAGTGCATTTGATCTGCCTGCTGAGCGTACAAGAAGTCGTAGAAATGAAGGCATGGTTAATCAGGGAGACAATAAGAAACAGTATGAACTGGATGTTGACCGCATTATGCAAGGAGAAGACAACCGAACAACACTTATGATAAAGAACATTCCCAACAAGTATACTTCAAAGATGCTATTGGCTGCGATTGATGAACGCCATCGAGGAACTTACGATTTCATTTATCTGCCAATTGATTTTAAGAACAAATGCAATGTGGGCTATGCGTTCATCAATATGACTGATCCTCGAATGATCGTTCCATTTTATCAGTCATTCAACGGGAAGAAATGGGAGAAGTTCAACAGTGAGAAGGTGGCATCACTCGCATATGCTCGAATTCAAGGAAAAGCTGCTCTGATTGCACATTTCCAGAATTCAAGCTTGATGAATGAGGATAAGCGATGCCGTCCCATTCTCTTCAACACTGATGGCCCAAATGCCGGTGATCAGGTGCCCTTCCCAATGGGGGTTAATGTTCGCACAAGACCGGGAAAAATTCGAGCAGTCACCCATGAGGAGAGCTATGTAGGGAGTCCACCAAGTTTTGGAGACGAAGAGCATTCTTGCAATGGAGAGACATCTGCAGGTTCCCGTTCTGCTAAGGAGTCAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1 RRM_2

Protein Analysis

979

Amino Acids

107.69

Weight (kDa)

6.04

Isoelectric Point (pI)

51.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 259 - 323 2.6e-10 RNA recognition motif
RRM_5 PF13893 341 - 414 1e-05 RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
RRM_1 PF00076 344 - 409 1.1e-09 RNA recognition motif
RRM_2 PF04059 802 - 898 1e-53 RNA recognition motif 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 581
Acc36I ACCTGC 2 cut(s) 2196, 2897
AccB1I GGYRCC 2 cut(s) 957, 2773
AccB7I CCANNNNNTGG 2 cut(s) 1999, 2867
AccI GTMKAC 1 cut(s) 2441
AccII CGCG 2 cut(s) 879, 1930
AciI CCGC 4 cut(s) 879, 961, 1930, 2380
AclWI GGATC 3 cut(s) 1105, 1384, 2558
AcoI YGGCCR 2 cut(s) 436, 1859
AcuI CTGAAG 2 cut(s) 1266, 1878
AcyI GRCGYC 1 cut(s) 958
AfaI GTAC 2 cut(s) 1422, 2308
AflII CTTAAG 1 cut(s) 232
AflIII ACRYGT 2 cut(s) 128, 2154
AjiI CACGTC 1 cut(s) 129
AjnI CCWGG 8 cut(s) 1044, 1077, 1243, 1801, 1846, 1972, 2086, 2251
AjuI GAANNNNNNNTTGG 2 cut(s) 1752, 1784
Alw21I GWGCWC 2 cut(s) 352, 1954
Alw26I GTCTC 6 cut(s) 119, 296, 1477, 2340, 2865, 2891
AlwI GGATC 3 cut(s) 1105, 1384, 2558
AlwNI CAGNNNCTG 1 cut(s) 1178
Ama87I CYCGRG 1 cut(s) 2066
AoxI GGCC 6 cut(s) 436, 507, 1309, 1859, 2260, 2752
AspLEI GCGC 1 cut(s) 960
AspS9I GGNCC 7 cut(s) 4, 151, 508, 619, 1361, 2185, 2753
AsuC2I CCSGG 5 cut(s) 195, 769, 1213, 1665, 2811
AsuHPI GGTGA 7 cut(s) 767, 1653, 1826, 1860, 2115, 2777, 2821
AvaI CYCGRG 1 cut(s) 2066
AvaII GGWCC 5 cut(s) 4, 151, 619, 1361, 2185
AxyI CCTNAGG 1 cut(s) 2163
BaeGI GKGCMC 2 cut(s) 1743, 2778
BalI TGGCCA 2 cut(s) 438, 1861
BanI GGYRCC 2 cut(s) 957, 2773
BanII GRGCYC 1 cut(s) 352
BbsI GAAGAC 1 cut(s) 2403
Bbv12I GWGCWC 2 cut(s) 352, 1954
BbvCI CCTCAGC 1 cut(s) 1839
BceAI ACGGC 2 cut(s) 1224, 2712
BciT130I CCWGG 8 cut(s) 1046, 1079, 1245, 1803, 1848, 1974, 2088, 2253
BclI TGATCA 1 cut(s) 2767
BcnI CCSGG 5 cut(s) 195, 769, 1213, 1665, 2811
BcoDI GTCTC 6 cut(s) 119, 296, 1477, 2340, 2865, 2891
BfaI CTAG 4 cut(s) 597, 1329, 1479, 1947
BfoI RGCGCY 1 cut(s) 961
BfrI CTTAAG 1 cut(s) 232
BfuAI ACCTGC 2 cut(s) 2196, 2897
BglII AGATCT 1 cut(s) 284
BlpI GCTNAGC 1 cut(s) 2301
Bme18I GGWCC 5 cut(s) 4, 151, 619, 1361, 2185
BmeT110I CYCGRG 1 cut(s) 2066
BmgBI CACGTC 1 cut(s) 129
BmgT120I GGNCC 7 cut(s) 4, 151, 508, 619, 1361, 2185, 2753
BmiI GGNNCC 3 cut(s) 959, 2775, 2911
BmsI GCATC 7 cut(s) 199, 317, 883, 2081, 2442, 2648, 2714
BpiI GAAGAC 1 cut(s) 2403
BpmI CTGGAG 3 cut(s) 143, 576, 1266
Bpu10I CCTNAGC 2 cut(s) 1839, 2922
Bpu1102I GCTNAGC 1 cut(s) 2301
BpuEI CTTGAG 1 cut(s) 1250
BpuMI CCSGG 5 cut(s) 195, 769, 1213, 1665, 2811
BsaBI GATNNNNATC 2 cut(s) 1017, 2273
BsaHI GRCGYC 1 cut(s) 958
BsaI GGTCTC 1 cut(s) 1477
BsaJI CCNNGG 3 cut(s) 1585, 1972, 2067
BsaXI ACNNNNNCTCC 2 cut(s) 1259, 1289
Bse118I RCCGGY 2 cut(s) 1778, 2762
Bse1I ACTGG 6 cut(s) 676, 1354, 1364, 1389, 2012, 2373
Bse21I CCTNAGG 1 cut(s) 2163
Bse3DI GCAATG 5 cut(s) 730, 1675, 1789, 2539, 2896
Bse8I GATNNNNATC 2 cut(s) 1017, 2273
BseBI CCWGG 8 cut(s) 1046, 1079, 1245, 1803, 1848, 1974, 2088, 2253
BseDI CCNNGG 3 cut(s) 1585, 1972, 2067
BseGI GGATG 9 cut(s) 154, 190, 648, 1581, 1603, 1900, 1918, 2083, 2377
BseJI GATNNNNATC 2 cut(s) 1017, 2273
BseMI GCAATG 5 cut(s) 730, 1675, 1789, 2539, 2896
BseMII CTCAG 8 cut(s) 365, 581, 804, 996, 1499, 1853, 2177, 2292
BseNI ACTGG 6 cut(s) 676, 1354, 1364, 1389, 2012, 2373
BseRI GAGGAG 3 cut(s) 13, 626, 2852
BseSI GKGCMC 2 cut(s) 1743, 2778
Bsh1236I CGCG 2 cut(s) 879, 1930
BshFI GGCC 6 cut(s) 438, 509, 1311, 1861, 2262, 2754
BshNI GGYRCC 2 cut(s) 957, 2773
BsiHKAI GWGCWC 2 cut(s) 352, 1954
BsiHKCI CYCGRG 1 cut(s) 2066
BsiSI CCGG 7 cut(s) 195, 769, 1212, 1664, 1779, 2763, 2810
BslFI GGGAC 4 cut(s) 695, 1429, 2048, 2715
BsmAI GTCTC 6 cut(s) 119, 296, 1477, 2340, 2865, 2891
BsmBI CGTCTC 2 cut(s) 119, 2865
BsmFI GGGAC 4 cut(s) 695, 1429, 2048, 2715
BsmI GAATGC 2 cut(s) 1457, 2881
BsnI GGCC 6 cut(s) 438, 509, 1311, 1861, 2262, 2754
Bso31I GGTCTC 1 cut(s) 1477
BsoBI CYCGRG 1 cut(s) 2066
Bsp1286I GDGCHC 4 cut(s) 352, 1743, 1954, 2778
Bsp1407I TGTACA 1 cut(s) 1420
Bsp143I GATC 8 cut(s) 70, 284, 1110, 1376, 2290, 2563, 2574, 2767
Bsp1720I GCTNAGC 1 cut(s) 2301
Bsp19I CCATGG 1 cut(s) 1585
BspACI CCGC 4 cut(s) 879, 961, 1930, 2380
BspANI GGCC 6 cut(s) 438, 509, 1311, 1861, 2262, 2754
BspCNI CTCAG 8 cut(s) 364, 582, 805, 997, 1500, 1852, 2176, 2293
BspFNI CGCG 2 cut(s) 879, 1930
BspHI TCATGA 2 cut(s) 1627, 2277
BspLI GGNNCC 3 cut(s) 959, 2775, 2911
BspMAI CTGCAG 8 cut(s) 942, 1174, 1323, 1432, 1658, 1915, 2220, 2908
BspMI ACCTGC 2 cut(s) 2196, 2897
BspPI GGATC 3 cut(s) 1105, 1384, 2558
BspQI GCTCTTC 1 cut(s) 2871
BspT107I GGYRCC 2 cut(s) 957, 2773
BspTI CTTAAG 1 cut(s) 232
BspTNI GGTCTC 1 cut(s) 1477
BsrDI GCAATG 5 cut(s) 730, 1675, 1789, 2539, 2896
BsrFI RCCGGY 2 cut(s) 1778, 2762
BsrGI TGTACA 1 cut(s) 1420
BsrI ACTGG 6 cut(s) 676, 1354, 1364, 1389, 2012, 2373
BssAI RCCGGY 2 cut(s) 1778, 2762
BssECI CCNNGG 3 cut(s) 1585, 1972, 2067
BssMI GATC 8 cut(s) 70, 284, 1110, 1376, 2290, 2563, 2574, 2767
BssNAI GTATAC 1 cut(s) 2442
BssNI GRCGYC 1 cut(s) 958
BssT1I CCWWGG 1 cut(s) 1585
Bst1107I GTATAC 1 cut(s) 2442
Bst2UI CCWGG 8 cut(s) 1046, 1079, 1245, 1803, 1848, 1974, 2088, 2253
Bst4CI ACNGT 8 cut(s) 139, 432, 454, 662, 797, 1275, 2361, 2627
Bst6I CTCTTC 5 cut(s) 468, 1480, 2251, 2744, 2871
BstACI GRCGYC 1 cut(s) 958
BstAFI CTTAAG 1 cut(s) 232
BstAPI GCANNNNNTGC 2 cut(s) 1178, 1439
BstAUI TGTACA 1 cut(s) 1420
BstDEI CTNAG 9 cut(s) 351, 590, 813, 1005, 1508, 1839, 2163, 2301, 2922
BstDSI CCRYGG 1 cut(s) 1585
BstENI CCTNNNNNAGG 1 cut(s) 2256
BstF5I GGATG 9 cut(s) 154, 190, 648, 1581, 1603, 1900, 1918, 2083, 2377
BstFNI CGCG 2 cut(s) 879, 1930
BstH2I RGCGCY 1 cut(s) 961
BstHHI GCGC 1 cut(s) 960
BstKTI GATC 8 cut(s) 73, 287, 1113, 1379, 2293, 2566, 2577, 2770
BstMAI GTCTC 6 cut(s) 119, 296, 1477, 2340, 2865, 2891
BstMBI GATC 8 cut(s) 70, 284, 1110, 1376, 2290, 2563, 2574, 2767
BstNI CCWGG 8 cut(s) 1046, 1079, 1245, 1803, 1848, 1974, 2088, 2253
BstNSI RCATGY 1 cut(s) 2158
BstSLI GKGCMC 2 cut(s) 1743, 2778
BstUI CGCG 2 cut(s) 879, 1930
BstV2I GAAGAC 1 cut(s) 2403
BstX2I RGATCY 2 cut(s) 284, 1110
BstXI CCANNNNNNTGG 1 cut(s) 1085
BstYI RGATCY 2 cut(s) 284, 1110
BstZ17I GTATAC 1 cut(s) 2442
Bsu36I CCTNAGG 1 cut(s) 2163
BsuRI GGCC 6 cut(s) 438, 509, 1311, 1861, 2262, 2754
BtgI CCRYGG 1 cut(s) 1585
BtgZI GCGATG 3 cut(s) 860, 941, 2737
BtrI CACGTC 1 cut(s) 129
BtsCI GGATG 9 cut(s) 154, 190, 648, 1581, 1603, 1900, 1918, 2083, 2377
BtsI GCAGTG 3 cut(s) 219, 637, 935
BtsIMutI CAGTG 9 cut(s) 144, 219, 428, 637, 935, 1347, 2019, 2632, 2745
BveI ACCTGC 2 cut(s) 2196, 2897
CaiI CAGNNNCTG 1 cut(s) 1178
CciI TCATGA 2 cut(s) 1627, 2277
CfoI GCGC 1 cut(s) 960
Cfr10I RCCGGY 2 cut(s) 1778, 2762
Cfr13I GGNCC 7 cut(s) 4, 151, 508, 619, 1361, 2185, 2753
CseI GACGC 2 cut(s) 1184, 1431
Csp6I GTAC 2 cut(s) 1421, 2307
CviQI GTAC 2 cut(s) 1421, 2307
DdeI CTNAG 9 cut(s) 351, 590, 813, 1005, 1508, 1839, 2163, 2301, 2922
DinI GGCGCC 1 cut(s) 959
DpnI GATC 8 cut(s) 72, 286, 1112, 1378, 2292, 2565, 2576, 2769
DpnII GATC 8 cut(s) 70, 284, 1110, 1376, 2290, 2563, 2574, 2767
DrdI GACNNNNNNGTC 1 cut(s) 581
DseDI GACNNNNNNGTC 1 cut(s) 581
EaeI YGGCCR 2 cut(s) 436, 1859
Eam1104I CTCTTC 5 cut(s) 468, 1480, 2251, 2744, 2871
EarI CTCTTC 5 cut(s) 468, 1480, 2251, 2744, 2871
Ecl136II GAGCTC 1 cut(s) 350
Eco130I CCWWGG 1 cut(s) 1585
Eco24I GRGCYC 1 cut(s) 352
Eco31I GGTCTC 1 cut(s) 1477
Eco32I GATATC 1 cut(s) 894
Eco47I GGWCC 5 cut(s) 4, 151, 619, 1361, 2185
Eco53kI GAGCTC 1 cut(s) 350
Eco57I CTGAAG 2 cut(s) 1266, 1878
Eco81I CCTNAGG 1 cut(s) 2163
Eco88I CYCGRG 1 cut(s) 2066
EcoICRI GAGCTC 1 cut(s) 350
EcoNI CCTNNNNNAGG 1 cut(s) 2256
EcoRI GAATTC 3 cut(s) 142, 2658, 2695
EcoRII CCWGG 8 cut(s) 1044, 1077, 1243, 1801, 1846, 1972, 2086, 2251
EcoRV GATATC 1 cut(s) 894
EcoT14I CCWWGG 1 cut(s) 1585
EcoT22I ATGCAT 2 cut(s) 1459, 1989
EcoT38I GRGCYC 1 cut(s) 352
EgeI GGCGCC 1 cut(s) 959
EheI GGCGCC 1 cut(s) 959
ErhI CCWWGG 1 cut(s) 1585
Esp3I CGTCTC 2 cut(s) 119, 2865
FaqI GGGAC 4 cut(s) 695, 1429, 2048, 2715
FauI CCCGC 1 cut(s) 1923
FauNDI CATATG 4 cut(s) 1747, 1962, 2050, 2650
FbaI TGATCA 1 cut(s) 2767
FblI GTMKAC 1 cut(s) 2441
FokI GGATG 9 cut(s) 141, 177, 655, 1568, 1590, 1887, 1905, 2070, 2384
FriOI GRGCYC 1 cut(s) 352
FspBI CTAG 4 cut(s) 597, 1329, 1479, 1947
GlaI GCGC 1 cut(s) 959
GsuI CTGGAG 3 cut(s) 143, 576, 1266
HaeII RGCGCY 1 cut(s) 961
HaeIII GGCC 6 cut(s) 438, 509, 1311, 1861, 2262, 2754
HapII CCGG 7 cut(s) 195, 769, 1212, 1664, 1779, 2763, 2810
HgaI GACGC 2 cut(s) 1184, 1431
HhaI GCGC 1 cut(s) 960
Hin1I GRCGYC 1 cut(s) 958
Hin6I GCGC 1 cut(s) 958
HinP1I GCGC 1 cut(s) 958
HincII GTYRAC 3 cut(s) 1651, 1789, 2377
HindII GTYRAC 3 cut(s) 1651, 1789, 2377
HindIII AAGCTT 4 cut(s) 480, 965, 1226, 2701
HpaI GTTAAC 1 cut(s) 1789
HpaII CCGG 7 cut(s) 195, 769, 1212, 1664, 1779, 2763, 2810
HphI GGTGA 7 cut(s) 767, 1653, 1826, 1860, 2115, 2777, 2821
Hpy166II GTNNAC 8 cut(s) 758, 1423, 1651, 1661, 1789, 2377, 2442, 2858
Hpy8I GTNNAC 8 cut(s) 758, 1423, 1651, 1661, 1789, 2377, 2442, 2858
Hpy99I CGWCG 2 cut(s) 31, 421
HpyCH4III ACNGT 8 cut(s) 139, 432, 454, 662, 797, 1275, 2361, 2627
HpyCH4IV ACGT 2 cut(s) 128, 1385
HpyF3I CTNAG 9 cut(s) 351, 590, 813, 1005, 1508, 1839, 2163, 2301, 2922
HpySE526I ACGT 2 cut(s) 128, 1385
Hsp92I GRCGYC 1 cut(s) 958
HspAI GCGC 1 cut(s) 958
KasI GGCGCC 1 cut(s) 957
KroI GCCGGC 1 cut(s) 1778
KroNI GCCGGC 1 cut(s) 1780
Ksp22I TGATCA 1 cut(s) 2767
KspAI GTTAAC 1 cut(s) 1789
Kzo9I GATC 8 cut(s) 70, 284, 1110, 1376, 2290, 2563, 2574, 2767
LguI GCTCTTC 1 cut(s) 2871
LmnI GCTCC 7 cut(s) 218, 278, 347, 388, 1260, 1267, 1736
LweI GCATC 7 cut(s) 199, 317, 883, 2081, 2442, 2648, 2714
MaeI CTAG 4 cut(s) 597, 1329, 1479, 1947
MaeII ACGT 2 cut(s) 128, 1385
MaeIII GTNAC 5 cut(s) 102, 559, 797, 1381, 2827
MalI GATC 8 cut(s) 72, 286, 1112, 1378, 2292, 2565, 2576, 2769
MboI GATC 8 cut(s) 70, 284, 1110, 1376, 2290, 2563, 2574, 2767
MfeI CAATTG 2 cut(s) 2193, 2511
MflI RGATCY 2 cut(s) 284, 1110
MhlI GDGCHC 4 cut(s) 352, 1743, 1954, 2778
MlsI TGGCCA 2 cut(s) 438, 1861
MluNI TGGCCA 2 cut(s) 438, 1861
Mly113I GGCGCC 1 cut(s) 958
MlyI GAGTC 5 cut(s) 677, 1334, 1556, 2863, 2936
MmeI TCCRAC 4 cut(s) 366, 597, 610, 616
Mox20I TGGCCA 2 cut(s) 438, 1861
Mph1103I ATGCAT 2 cut(s) 1459, 1989
MroNI GCCGGC 1 cut(s) 1778
MscI TGGCCA 2 cut(s) 438, 1861
MslI CAYNNNNRTG 5 cut(s) 468, 750, 1746, 2205, 2555
Msp20I TGGCCA 2 cut(s) 438, 1861
MspCI CTTAAG 1 cut(s) 232
MspI CCGG 7 cut(s) 195, 769, 1212, 1664, 1779, 2763, 2810
MunI CAATTG 2 cut(s) 2193, 2511
Mva1269I GAATGC 2 cut(s) 1457, 2881
MvaI CCWGG 8 cut(s) 1046, 1079, 1245, 1803, 1848, 1974, 2088, 2253
MvnI CGCG 2 cut(s) 879, 1930
NaeI GCCGGC 1 cut(s) 1780
NarI GGCGCC 1 cut(s) 958
NciI CCSGG 5 cut(s) 195, 769, 1213, 1665, 2811
NcoI CCATGG 1 cut(s) 1585
NdeI CATATG 4 cut(s) 1747, 1962, 2050, 2650
NdeII GATC 8 cut(s) 70, 284, 1110, 1376, 2290, 2563, 2574, 2767
NgoMIV GCCGGC 1 cut(s) 1778
NlaIV GGNNCC 3 cut(s) 959, 2775, 2911
NmuCI GTSAC 2 cut(s) 102, 2827
NsiI ATGCAT 2 cut(s) 1459, 1989
NspI RCATGY 1 cut(s) 2158
PagI TCATGA 2 cut(s) 1627, 2277
PciI ACATGT 1 cut(s) 2154
PciSI GCTCTTC 1 cut(s) 2871
PctI GAATGC 2 cut(s) 1457, 2881
PdiI GCCGGC 1 cut(s) 1780
PfeI GAWTC 9 cut(s) 272, 488, 809, 1049, 1517, 1528, 2060, 2109, 2269
PflMI CCANNNNNTGG 2 cut(s) 1999, 2867
PfoI TCCNGGA 5 cut(s) 767, 1243, 1801, 1846, 2251
PleI GAGTC 5 cut(s) 677, 1334, 1555, 2862, 2935
PluTI GGCGCC 1 cut(s) 961
PpsI GAGTC 5 cut(s) 677, 1334, 1555, 2862, 2935
PscI ACATGT 1 cut(s) 2154
Psp124BI GAGCTC 1 cut(s) 352
Psp6I CCWGG 8 cut(s) 1044, 1077, 1243, 1801, 1846, 1972, 2086, 2251
PspGI CCWGG 8 cut(s) 1044, 1077, 1243, 1801, 1846, 1972, 2086, 2251
PspN4I GGNNCC 3 cut(s) 959, 2775, 2911
PspPI GGNCC 7 cut(s) 4, 151, 508, 619, 1361, 2185, 2753
PstI CTGCAG 8 cut(s) 942, 1174, 1323, 1432, 1658, 1915, 2220, 2908
PstNI CAGNNNCTG 1 cut(s) 1178
PsuI RGATCY 2 cut(s) 284, 1110
RsaI GTAC 2 cut(s) 1422, 2308
RsaNI GTAC 2 cut(s) 1421, 2307
RseI CAYNNNNRTG 5 cut(s) 468, 750, 1746, 2205, 2555
SacI GAGCTC 1 cut(s) 352
SapI GCTCTTC 1 cut(s) 2871
Sau3AI GATC 8 cut(s) 70, 284, 1110, 1376, 2290, 2563, 2574, 2767
Sau96I GGNCC 7 cut(s) 4, 151, 508, 619, 1361, 2185, 2753
SchI GAGTC 5 cut(s) 677, 1334, 1556, 2863, 2936
SduI GDGCHC 4 cut(s) 352, 1743, 1954, 2778
SfaNI GCATC 7 cut(s) 199, 317, 883, 2081, 2442, 2648, 2714
SfoI GGCGCC 1 cut(s) 959
SinI GGWCC 5 cut(s) 4, 151, 619, 1361, 2185
SmiMI CAYNNNNRTG 5 cut(s) 468, 750, 1746, 2205, 2555
SmlI CTYRAG 3 cut(s) 8, 232, 1229
SmoI CTYRAG 3 cut(s) 8, 232, 1229
SsiI CCGC 4 cut(s) 879, 961, 1930, 2380
SspDI GGCGCC 1 cut(s) 957
SspMI CTAG 4 cut(s) 597, 1329, 1479, 1947
SstI GAGCTC 1 cut(s) 352
StyI CCWWGG 1 cut(s) 1585
TaaI ACNGT 8 cut(s) 139, 432, 454, 662, 797, 1275, 2361, 2627
TaiI ACGT 2 cut(s) 131, 1388
TaqI TCGA 6 cut(s) 254, 419, 2482, 2569, 2656, 2821
TatI WGTACW 1 cut(s) 1420
TauI GCSGC 1 cut(s) 963
TfiI GAWTC 9 cut(s) 272, 488, 809, 1049, 1517, 1528, 2060, 2109, 2269
TscAI CASTG 9 cut(s) 144, 219, 435, 637, 942, 1354, 2019, 2632, 2752
TseFI GTSAC 2 cut(s) 102, 2827
Tsp45I GTSAC 2 cut(s) 102, 2827
TspGWI ACGGA 2 cut(s) 1103, 1273
TspRI CASTG 9 cut(s) 144, 219, 435, 637, 942, 1354, 2019, 2632, 2752
Van91I CCANNNNNTGG 2 cut(s) 1999, 2867
Vha464I CTTAAG 1 cut(s) 232
VpaK11BI GGWCC 5 cut(s) 4, 151, 619, 1361, 2185
XagI CCTNNNNNAGG 1 cut(s) 2256
XceI RCATGY 1 cut(s) 2158
XmiI GTMKAC 1 cut(s) 2441
XspI CTAG 4 cut(s) 597, 1329, 1479, 1947
Zsp2I ATGCAT 2 cut(s) 1459, 1989
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.