Rh4DG290100

Protein MEI2-like 4 isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0001846
Physical Location & Seq
Reverse (-)
51105090 .. 51112948
7859 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG290100.1

Sequence Viewer

Length: 2754 bp
ATGCCATCTGAAGTAATGGACATTAAGGGCCTGTCGTCTTCTTCGTTCTTCTCCGAAGATTTCCCAAACGAGAGGCAGGTTGGGTTTTGGAAGTCAGATAACATGCCAGATAAACATGCCGGTGAGAAGTCACTTGCATCATCATCTCTTGAGAAGTGTCAGACGGTGAAATCTCTGGATCACCCTGATTTTTTCTTAATGCCAGAACAAAAGGTGAAACCCAGCTTCAATAGACAAGCAGTTGGGGCAGAGAGAGCCTTAAGTCATTCGTTGAATTTATCGAGAGCCGTGAGCCTTGACTCGGGAGTAATATCGAATGTGAATGTTGAGACAGCTTCTTACTATGCGGAAGGTGGCAAAGTCAATATGATGGGAACGCAGTATGAGAGTAGCCTTTTCTCGAGTTCATTATCTGAGTTGTTTAGTAGGAAGTTGAGACTATCGTCAAACAATACACAATATGGACATTCTGTTGATAGTGTTGCCTCAAACTATGAGGAAGAGGAAGTTTTTGAGTCCCTTGAAGAAATGGAGGCCCAAACCATCGGAAACCTTCTCCCTGATGATGATGAGTTGCTTTCTGGAGTTACCGATGGGCTCGAGTATAATGTTCCACTCACTGCTGGGGATGATATTGAGGAGTTGGATTTTTTTAGCAGTAGTGGAGGGATGGATTTGGGAGACGATGGTCTGTCAGCTGGACTAAACAATTCTGATTGTCCTGGAGGAGTTTCGAATGTTTTGCAATGCAGTGGTTCAGTAGCTGGAGAGCACCCTAATGGTGAACACCCTTCTAGGACATTGTTTGTGAGAAATATAAATAGCAACATTGAAGACTCTGAGTTGAGAACCCTGTTTGAGCAATACGGAGACATTCGGACTCTTTATACAGCCTGCAAACACCGTGGGTTTGTTATGATATCATATTATGATATTAGAGCAGCCTGGAATGCGATGAAAGCACTCCAGAACAAACCATTGAGGCGCCGGAAGCTTGACATTCATTACTCGATTCCAAAGGACAATCCTTCTGAGAAAGATGTTAATCAGGGCACTCTTGTGGTGTTTAACCTTGATTCTTCTGTTTCTAATCATGAACTTAGTGAGCTTTTTGGTGTCTATGGTGAAATCAAAGAGATCCGTGAAACACCAAATAGAAGTCATCACAAGTTTATTGAATTTTATGATGTTAGAGCTGCAGAATCTGCTCTTAATGCCATGAACAGGAGTGATATAGCCGGGAAGAGGATTAAGCTTGAGCCAAGTCGTCCTGGAGGTGCAAAAAGGAGCTCAGGGCTGCAGTTACCTCAAGAGTTAGAGCAGGATGATTGTGGCCTTTATTTTCAGCAGAGTAGTCCTCCTAGTAACTCTGTTACTGGTTTCTCTGGTGCAATTACATCCTCTTGCACAGATAATGGAGCTATTATGACTGTACACTCTGCAGCTCCATCTTTTGAGAACATGTTCCATCATGGGATCTCATCTAGTGTACCCAACAGCCTATCTTCTATGATCAGAGTTGAATCAGCAGTCAGTAAGTCTGGCCTTACTGAGTCCATTCATTCAGCAAGCCCGCTGAAATTTGACATCCACGGCTCACCAGCTTTTCATCCTCATTCACTTCCAGAGTATCATAATGGTTTAACTAATGGCATCAACTGCAATTCAACAGGCAGTGTATCTGCAAGTATCAATGTCAGACCACCAGATAACAGGCACTTTACCAGAGTCTCAAGTGGGCACGCACGTGAACTTAATGATAGTGCTTTTGGATCTATGGGTAACGTGAACTGCCCTATTCCTGGACATCATTATGCATGGAACAATTCCTTTCAGTCTCAGGCTCCAGGAATGATGTGGCCAAACTCACCATCATTTGTTAATGGAATTTCAGCAGCCCATCCCTCACAGCGGATGCATGGACTTCCTAGAGCACCATCTCATATGTTAAACGCAGCAATGCCCATAAATAAACATCATGTGGGTTCAGCACCAGGGGCTAACCCAATGTGGGACCGGAGACAAGCATATGCAGGGGAATCCCCCGAGTCATCTGGATTTCATCCAGGCTCTCTTGGGAATATGAGAATGCCAAATAACTCACCTCATTCGTTGGAATTTGTTTCTCATAACATGTTTCCTCATGTTAATGGAAATGGCATGGACCTGCCAATTCCCCACAAAAATGTAGGACTCCAGGCCCATCATCAGAGGTGCATGATGTATCCTGGAAGAAGTCAGATGGTTCCTGTTATGAATTCATTTGATCAACCTACTGAACGTCCAAGAAGTCGTAGAAATGAAGGCAGCTCTAATCAGGTTGACAACAAGAAACAGTATGAACTTGATATTGACCGCATTATGCGAGGGGATGACAACAGAACAACACTTATGATAAAGAATATTCCTAACAAGTATACCTCAAAGATGCTTTTGGCTGCGATCGATGAGCGCCATCGTGGAACATATGATTTCATTTACCTGCCAATTGATTTTAAGAACAAATGCAATGTGGGGTATGCATTCATCAATATGACTGATCCGAGACAGATTGTTCCATTTTATCAGTCATTCAATGGGAAGAAATGGGAGAAGTTTAATAGTGAGAAGGTAGCATCAATTGCTTATGCTCGAATTCAAGGCAAAGCTGCTCTGATTGCACATTTCCAGAACTCAAGCCTGATGAATGAGGATAAGCGATGCCGTCCCATTCTCTTCAATACTGATGGCCCCAATGCAGGTGATCAGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1 RRM_2

Protein Analysis

917

Amino Acids

101.33

Weight (kDa)

6.08

Isoelectric Point (pI)

52.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 268 - 332 1.6e-09 RNA recognition motif
RRM_5 PF13893 350 - 426 1.7e-06 RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
RRM_1 PF00076 353 - 418 7.4e-10 RNA recognition motif
RRM_2 PF04059 794 - 890 7.7e-54 RNA recognition motif 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2729
Acc36I ACCTGC 4 cut(s) 67, 2175, 2490, 2729
AccB1I GGYRCC 1 cut(s) 984
AccI GTMKAC 1 cut(s) 2417
AciI CCGC 4 cut(s) 347, 1574, 1912, 2356
AclWI GGATC 5 cut(s) 186, 1132, 1484, 1780, 2534
AcoI YGGCCR 1 cut(s) 1859
AcsI RAATTY 7 cut(s) 274, 1178, 1580, 1887, 2117, 2257, 2634
AcuI CTGAAG 1 cut(s) 30
AcvI CACGTG 1 cut(s) 1748
AcyI GRCGYC 1 cut(s) 985
AfaI GTAC 2 cut(s) 1434, 1491
AfiI CCNNNNNNNGG 7 cut(s) 301, 1224, 1245, 1802, 1911, 2011, 2738
AflII CTTAAG 1 cut(s) 259
AflIII ACRYGT 2 cut(s) 1461, 2133
AjnI CCWGG 9 cut(s) 721, 944, 1270, 1801, 1846, 1993, 2065, 2196, 2227
AleI CACNNNNGTG 2 cut(s) 1058, 1746
Alw21I GWGCWC 3 cut(s) 774, 1292, 1936
Alw26I GTCTC 8 cut(s) 323, 430, 675, 864, 1735, 1842, 2014, 2539
AlwI GGATC 5 cut(s) 186, 1132, 1484, 1780, 2534
AlwNI CAGNNNCTG 2 cut(s) 764, 1205
Ama87I CYCGRG 4 cut(s) 301, 400, 599, 2045
AoxI GGCC 7 cut(s) 28, 534, 1333, 1543, 1859, 2199, 2728
ApeKI GCWGC 9 cut(s) 941, 1196, 1297, 1442, 1895, 1955, 2307, 2438, 2648
ApoI RAATTY 7 cut(s) 274, 1178, 1580, 1887, 2117, 2257, 2634
Asp700I GAANNNNTTC 1 cut(s) 1463
AspLEI GCGC 2 cut(s) 987, 2454
AspS9I GGNCC 6 cut(s) 28, 535, 2014, 2164, 2200, 2729
AsuC2I CCSGG 1 cut(s) 1240
AsuII TTCGAA 1 cut(s) 734
AvaI CYCGRG 4 cut(s) 301, 400, 599, 2045
AvaII GGWCC 2 cut(s) 2014, 2164
BaeGI GKGCMC 2 cut(s) 1055, 1743
BalI TGGCCA 1 cut(s) 1861
BanI GGYRCC 1 cut(s) 984
BanII GRGCYC 2 cut(s) 600, 1292
BbrPI CACGTG 1 cut(s) 1748
BbsI GAAGAC 2 cut(s) 30, 840
Bbv12I GWGCWC 3 cut(s) 774, 1292, 1936
BbvI GCAGC 9 cut(s) 953, 1183, 1284, 1454, 1907, 1967, 2319, 2425, 2635
BceAI ACGGC 3 cut(s) 272, 1609, 2688
BcgI CGANNNNNNTGC 2 cut(s) 724, 758
BciT130I CCWGG 9 cut(s) 723, 946, 1272, 1803, 1848, 1995, 2067, 2198, 2229
BciVI GTATCC 1 cut(s) 2235
BclI TGATCA 3 cut(s) 1512, 2266, 2743
BcnI CCSGG 1 cut(s) 1240
BcoDI GTCTC 8 cut(s) 323, 430, 675, 864, 1735, 1842, 2014, 2539
BfaI CTAG 4 cut(s) 795, 1362, 1485, 1929
BfmI CTRYAG 3 cut(s) 1197, 1298, 1440
BfoI RGCGCY 2 cut(s) 988, 2455
BfrI CTTAAG 1 cut(s) 259
BfuAI ACCTGC 4 cut(s) 67, 2175, 2490, 2729
BfuI GTATCC 1 cut(s) 2235
BisI GCNGC 9 cut(s) 942, 1197, 1298, 1443, 1896, 1956, 2308, 2439, 2649
BlsI GCNGC 9 cut(s) 943, 1198, 1299, 1444, 1897, 1957, 2309, 2440, 2650
Bme18I GGWCC 2 cut(s) 2014, 2164
BmeT110I CYCGRG 4 cut(s) 301, 400, 599, 2045
BmgT120I GGNCC 6 cut(s) 28, 535, 2014, 2164, 2200, 2729
BmiI GGNNCC 5 cut(s) 986, 1845, 2015, 2247, 2731
BmsI GCATC 6 cut(s) 146, 1662, 1905, 2418, 2624, 2690
BoxI GACNNNNGTC 2 cut(s) 442, 687
BpiI GAAGAC 2 cut(s) 30, 840
BplI GAGNNNNNCTC 2 cut(s) 1342, 1374
BpmI CTGGAG 7 cut(s) 603, 744, 786, 950, 1293, 1830, 2180
Bpu10I CCTNAGC 1 cut(s) 1291
Bpu14I TTCGAA 1 cut(s) 734
BpuEI CTTGAG 5 cut(s) 170, 1277, 1293, 1717, 2659
BpuMI CCSGG 1 cut(s) 1240
Bsa29I ATCGAT 1 cut(s) 2445
BsaAI YACGTR 1 cut(s) 1748
BsaBI GATNNNNATC 1 cut(s) 1044
BsaHI GRCGYC 1 cut(s) 985
BsaJI CCNNGG 3 cut(s) 904, 1591, 1994
BsaWI WCCGGW 1 cut(s) 2016
Bsc4I CCNNNNNNNGG 7 cut(s) 301, 1224, 1245, 1802, 1911, 2011, 2738
Bse118I RCCGGY 1 cut(s) 119
Bse1I ACTGG 1 cut(s) 1381
Bse3DI GCAATG 3 cut(s) 752, 1965, 2515
Bse8I GATNNNNATC 1 cut(s) 1044
BseBI CCWGG 9 cut(s) 723, 946, 1272, 1803, 1848, 1995, 2067, 2198, 2229
BseCI ATCGAT 1 cut(s) 2445
BseDI CCNNGG 3 cut(s) 904, 1591, 1994
BseJI GATNNNNATC 1 cut(s) 1044
BseLI CCNNNNNNNGG 7 cut(s) 301, 1224, 1245, 1802, 1911, 2011, 2738
BseMI GCAATG 3 cut(s) 752, 1965, 2515
BseMII CTCAG 6 cut(s) 405, 831, 1023, 1305, 1542, 1853
BseNI ACTGG 1 cut(s) 1381
BseRI GAGGAG 2 cut(s) 653, 741
BseSI GKGCMC 2 cut(s) 1055, 1743
BseXI GCAGC 9 cut(s) 953, 1183, 1284, 1454, 1907, 1967, 2319, 2425, 2635
BseYI CCCAGC 2 cut(s) 221, 623
Bsh1285I CGRYCG 1 cut(s) 2445
BshFI GGCC 7 cut(s) 30, 536, 1335, 1545, 1861, 2201, 2730
BshNI GGYRCC 1 cut(s) 984
BshVI ATCGAT 1 cut(s) 2445
BsiEI CGRYCG 1 cut(s) 2445
BsiHKAI GWGCWC 3 cut(s) 774, 1292, 1936
BsiHKCI CYCGRG 4 cut(s) 301, 400, 599, 2045
BsiSI CCGG 4 cut(s) 120, 988, 1239, 2017
BslFI GGGAC 3 cut(s) 502, 2027, 2691
BslI CCNNNNNNNGG 7 cut(s) 301, 1224, 1245, 1802, 1911, 2011, 2738
BsmAI GTCTC 8 cut(s) 323, 430, 675, 864, 1735, 1842, 2014, 2539
BsmBI CGTCTC 1 cut(s) 675
BsmFI GGGAC 3 cut(s) 502, 2027, 2691
BsmI GAATGC 3 cut(s) 955, 2094, 2522
BsnI GGCC 7 cut(s) 30, 536, 1335, 1545, 1861, 2201, 2730
BsoBI CYCGRG 4 cut(s) 301, 400, 599, 2045
Bsp119I TTCGAA 1 cut(s) 734
Bsp1286I GDGCHC 6 cut(s) 600, 774, 1055, 1292, 1743, 1936
Bsp1407I TGTACA 1 cut(s) 1432
Bsp143I GATC 9 cut(s) 178, 1137, 1476, 1512, 1772, 2266, 2442, 2539, 2743
BspACI CCGC 4 cut(s) 347, 1574, 1912, 2356
BspANI GGCC 7 cut(s) 30, 536, 1335, 1545, 1861, 2201, 2730
BspCNI CTCAG 6 cut(s) 406, 832, 1024, 1304, 1543, 1852
BspDI ATCGAT 1 cut(s) 2445
BspHI TCATGA 1 cut(s) 1093
BspLI GGNNCC 5 cut(s) 986, 1845, 2015, 2247, 2731
BspMAI CTGCAG 3 cut(s) 1201, 1302, 1444
BspMI ACCTGC 4 cut(s) 67, 2175, 2490, 2729
BspPI GGATC 5 cut(s) 186, 1132, 1484, 1780, 2534
BspT104I TTCGAA 1 cut(s) 734
BspT107I GGYRCC 1 cut(s) 984
BspTI CTTAAG 1 cut(s) 259
BsrDI GCAATG 3 cut(s) 752, 1965, 2515
BsrFI RCCGGY 1 cut(s) 119
BsrGI TGTACA 1 cut(s) 1432
BsrI ACTGG 1 cut(s) 1381
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 3 cut(s) 904, 1591, 1994
BssMI GATC 9 cut(s) 178, 1137, 1476, 1512, 1772, 2266, 2442, 2539, 2743
BssNAI GTATAC 1 cut(s) 2418
BssNI GRCGYC 1 cut(s) 985
Bst1107I GTATAC 1 cut(s) 2418
Bst2UI CCWGG 9 cut(s) 723, 946, 1272, 1803, 1848, 1995, 2067, 2198, 2229
Bst4CI ACNGT 4 cut(s) 166, 905, 1432, 2337
Bst6I CTCTTC 3 cut(s) 495, 1238, 2720
BstACI GRCGYC 1 cut(s) 985
BstAFI CTTAAG 1 cut(s) 259
BstAPI GCANNNNNTGC 3 cut(s) 1205, 1659, 2621
BstAUI TGTACA 1 cut(s) 1432
BstBAI YACGTR 1 cut(s) 1748
BstBI TTCGAA 1 cut(s) 734
BstC8I GCNNGC 4 cut(s) 895, 1570, 1574, 1743
BstDEI CTNAG 7 cut(s) 414, 840, 1032, 1100, 1291, 1551, 1839
BstDSI CCRYGG 2 cut(s) 904, 1591
BstH2I RGCGCY 2 cut(s) 988, 2455
BstHHI GCGC 2 cut(s) 987, 2454
BstKTI GATC 9 cut(s) 181, 1140, 1479, 1515, 1775, 2269, 2445, 2542, 2746
BstMAI GTCTC 8 cut(s) 323, 430, 675, 864, 1735, 1842, 2014, 2539
BstMBI GATC 9 cut(s) 178, 1137, 1476, 1512, 1772, 2266, 2442, 2539, 2743
BstMCI CGRYCG 1 cut(s) 2445
BstNI CCWGG 9 cut(s) 723, 946, 1272, 1803, 1848, 1995, 2067, 2198, 2229
BstNSI RCATGY 4 cut(s) 106, 119, 1465, 2137
BstPAI GACNNNNGTC 2 cut(s) 442, 687
BstSFI CTRYAG 3 cut(s) 1197, 1298, 1440
BstSLI GKGCMC 2 cut(s) 1055, 1743
BstV1I GCAGC 9 cut(s) 953, 1183, 1284, 1454, 1907, 1967, 2319, 2425, 2635
BstV2I GAAGAC 2 cut(s) 30, 840
BstX2I RGATCY 3 cut(s) 1137, 1476, 1772
BstYI RGATCY 3 cut(s) 1137, 1476, 1772
BstZ17I GTATAC 1 cut(s) 2418
Bsu15I ATCGAT 1 cut(s) 2445
BsuI GTATCC 1 cut(s) 2235
BsuRI GGCC 7 cut(s) 30, 536, 1335, 1545, 1861, 2201, 2730
BsuTUI ATCGAT 1 cut(s) 2445
BtgI CCRYGG 2 cut(s) 904, 1591
BtgZI GCGATG 2 cut(s) 968, 2713
BtsI GCAGTG 3 cut(s) 618, 757, 1681
BtsIMutI CAGTG 3 cut(s) 618, 757, 1681
BveI ACCTGC 4 cut(s) 67, 2175, 2490, 2729
Cac8I GCNNGC 4 cut(s) 895, 1570, 1574, 1743
CaiI CAGNNNCTG 2 cut(s) 764, 1205
CciI TCATGA 1 cut(s) 1093
CfoI GCGC 2 cut(s) 987, 2454
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 6 cut(s) 28, 535, 2014, 2164, 2200, 2729
ClaI ATCGAT 1 cut(s) 2445
Csp6I GTAC 2 cut(s) 1433, 1490
CspCI CAANNNNNGTGG 2 cut(s) 886, 921
CviQI GTAC 2 cut(s) 1433, 1490
DdeI CTNAG 7 cut(s) 414, 840, 1032, 1100, 1291, 1551, 1839
DinI GGCGCC 1 cut(s) 986
DpnI GATC 9 cut(s) 180, 1139, 1478, 1514, 1774, 2268, 2444, 2541, 2745
DpnII GATC 9 cut(s) 178, 1137, 1476, 1512, 1772, 2266, 2442, 2539, 2743
EaeI YGGCCR 1 cut(s) 1859
Eam1104I CTCTTC 3 cut(s) 495, 1238, 2720
EarI CTCTTC 3 cut(s) 495, 1238, 2720
Ecl136II GAGCTC 1 cut(s) 1290
Eco24I GRGCYC 2 cut(s) 600, 1292
Eco32I GATATC 1 cut(s) 921
Eco47I GGWCC 2 cut(s) 2014, 2164
Eco53kI GAGCTC 1 cut(s) 1290
Eco57I CTGAAG 1 cut(s) 30
Eco72I CACGTG 1 cut(s) 1748
Eco88I CYCGRG 4 cut(s) 301, 400, 599, 2045
EcoICRI GAGCTC 1 cut(s) 1290
EcoO109I RGGNCCY 1 cut(s) 28
EcoRI GAATTC 2 cut(s) 2257, 2634
EcoRII CCWGG 9 cut(s) 721, 944, 1270, 1801, 1846, 1993, 2065, 2196, 2227
EcoRV GATATC 1 cut(s) 921
EcoT22I ATGCAT 3 cut(s) 1819, 1920, 2524
EcoT38I GRGCYC 2 cut(s) 600, 1292
EgeI GGCGCC 1 cut(s) 986
EheI GGCGCC 1 cut(s) 986
Esp3I CGTCTC 1 cut(s) 675
FaqI GGGAC 3 cut(s) 502, 2027, 2691
FauI CCCGC 1 cut(s) 1581
FauNDI CATATG 3 cut(s) 1944, 2029, 2467
FbaI TGATCA 3 cut(s) 1512, 2266, 2743
FblI GTMKAC 1 cut(s) 2417
Fnu4HI GCNGC 9 cut(s) 942, 1197, 1298, 1443, 1896, 1956, 2308, 2439, 2649
FriOI GRGCYC 2 cut(s) 600, 1292
Fsp4HI GCNGC 9 cut(s) 942, 1197, 1298, 1443, 1896, 1956, 2308, 2439, 2649
FspBI CTAG 4 cut(s) 795, 1362, 1485, 1929
GlaI GCGC 2 cut(s) 986, 2453
GluI GCNGC 9 cut(s) 942, 1197, 1298, 1443, 1896, 1956, 2308, 2439, 2649
GsaI CCCAGC 2 cut(s) 225, 627
GsuI CTGGAG 7 cut(s) 603, 744, 786, 950, 1293, 1830, 2180
HaeII RGCGCY 2 cut(s) 988, 2455
HaeIII GGCC 7 cut(s) 30, 536, 1335, 1545, 1861, 2201, 2730
HapII CCGG 4 cut(s) 120, 988, 1239, 2017
HhaI GCGC 2 cut(s) 987, 2454
Hin1I GRCGYC 1 cut(s) 985
Hin6I GCGC 2 cut(s) 985, 2452
HinP1I GCGC 2 cut(s) 985, 2452
HincII GTYRAC 1 cut(s) 2323
HindII GTYRAC 1 cut(s) 2323
HindIII AAGCTT 2 cut(s) 992, 1253
HpaII CCGG 4 cut(s) 120, 988, 1239, 2017
Hpy166II GTNNAC 7 cut(s) 785, 1435, 1490, 1751, 1789, 2323, 2418
Hpy8I GTNNAC 7 cut(s) 785, 1435, 1490, 1751, 1789, 2323, 2418
HpyAV CCTTC 6 cut(s) 344, 563, 801, 1038, 2297, 2602
HpyCH4III ACNGT 4 cut(s) 166, 905, 1432, 2337
HpyCH4IV ACGT 3 cut(s) 1747, 1785, 2281
HpyF3I CTNAG 7 cut(s) 414, 840, 1032, 1100, 1291, 1551, 1839
HpySE526I ACGT 3 cut(s) 1747, 1785, 2281
Hsp92I GRCGYC 1 cut(s) 985
HspAI GCGC 2 cut(s) 985, 2452
KasI GGCGCC 1 cut(s) 984
Ksp22I TGATCA 3 cut(s) 1512, 2266, 2743
Kzo9I GATC 9 cut(s) 178, 1137, 1476, 1512, 1772, 2266, 2442, 2539, 2743
LmnI GCTCC 4 cut(s) 1287, 1418, 1450, 1849
Lsp1109I GCAGC 9 cut(s) 953, 1183, 1284, 1454, 1907, 1967, 2319, 2425, 2635
LweI GCATC 6 cut(s) 146, 1662, 1905, 2418, 2624, 2690
MaeI CTAG 4 cut(s) 795, 1362, 1485, 1929
MaeII ACGT 3 cut(s) 1747, 1785, 2281
MaeIII GTNAC 6 cut(s) 129, 586, 1302, 1364, 1372, 1781
MalI GATC 9 cut(s) 180, 1139, 1478, 1514, 1774, 2268, 2444, 2541, 2745
MboI GATC 9 cut(s) 178, 1137, 1476, 1512, 1772, 2266, 2442, 2539, 2743
MfeI CAATTG 2 cut(s) 2487, 2619
MflI RGATCY 3 cut(s) 1137, 1476, 1772
MhlI GDGCHC 6 cut(s) 600, 774, 1055, 1292, 1743, 1936
MlsI TGGCCA 1 cut(s) 1861
MluNI TGGCCA 1 cut(s) 1861
Mly113I GGCGCC 1 cut(s) 985
MlyI GAGTC 8 cut(s) 293, 524, 830, 874, 1562, 1737, 2057, 2187
MmeI TCCRAC 2 cut(s) 624, 2094
Mox20I TGGCCA 1 cut(s) 1861
Mph1103I ATGCAT 3 cut(s) 1819, 1920, 2524
MroXI GAANNNNTTC 1 cut(s) 1463
MscI TGGCCA 1 cut(s) 1861
MslI CAYNNNNRTG 8 cut(s) 120, 777, 1058, 1746, 1812, 2148, 2184, 2531
Msp20I TGGCCA 1 cut(s) 1861
MspA1I CMGCKG 3 cut(s) 698, 1576, 1912
MspCI CTTAAG 1 cut(s) 259
MspI CCGG 4 cut(s) 120, 988, 1239, 2017
MunI CAATTG 2 cut(s) 2487, 2619
Mva1269I GAATGC 3 cut(s) 955, 2094, 2522
MvaI CCWGG 9 cut(s) 723, 946, 1272, 1803, 1848, 1995, 2067, 2198, 2229
NarI GGCGCC 1 cut(s) 985
NciI CCSGG 1 cut(s) 1240
NdeI CATATG 3 cut(s) 1944, 2029, 2467
NdeII GATC 9 cut(s) 178, 1137, 1476, 1512, 1772, 2266, 2442, 2539, 2743
NlaIV GGNNCC 5 cut(s) 986, 1845, 2015, 2247, 2731
NmuCI GTSAC 1 cut(s) 129
NsiI ATGCAT 3 cut(s) 1819, 1920, 2524
NspI RCATGY 4 cut(s) 106, 119, 1465, 2137
NspV TTCGAA 1 cut(s) 734
OliI CACNNNNGTG 2 cut(s) 1058, 1746
PaeR7I CTCGAG 2 cut(s) 400, 599
PagI TCATGA 1 cut(s) 1093
PaqCI CACCTGC 1 cut(s) 2729
PciI ACATGT 2 cut(s) 1461, 2133
PcsI WCGNNNNNNNCGW 1 cut(s) 41
PctI GAATGC 3 cut(s) 955, 2094, 2522
PdmI GAANNNNTTC 1 cut(s) 1463
PfeI GAWTC 5 cut(s) 1012, 1076, 1202, 1523, 2039
PfoI TCCNGGA 5 cut(s) 721, 1270, 1801, 1846, 2227
PkrI GCNGC 9 cut(s) 943, 1198, 1299, 1444, 1897, 1957, 2309, 2440, 2650
Ple19I CGATCG 1 cut(s) 2445
PleI GAGTC 8 cut(s) 293, 523, 830, 874, 1561, 1736, 2056, 2187
PluTI GGCGCC 1 cut(s) 988
PmaCI CACGTG 1 cut(s) 1748
PmlI CACGTG 1 cut(s) 1748
PpsI GAGTC 8 cut(s) 293, 523, 830, 874, 1561, 1736, 2056, 2187
Ppu21I YACGTR 1 cut(s) 1748
PscI ACATGT 2 cut(s) 1461, 2133
PshAI GACNNNNGTC 2 cut(s) 442, 687
Psp124BI GAGCTC 1 cut(s) 1292
Psp6I CCWGG 9 cut(s) 721, 944, 1270, 1801, 1846, 1993, 2065, 2196, 2227
PspCI CACGTG 1 cut(s) 1748
PspFI CCCAGC 2 cut(s) 221, 623
PspGI CCWGG 9 cut(s) 721, 944, 1270, 1801, 1846, 1993, 2065, 2196, 2227
PspN4I GGNNCC 5 cut(s) 986, 1845, 2015, 2247, 2731
PspPI GGNCC 6 cut(s) 28, 535, 2014, 2164, 2200, 2729
PspXI VCTCGAGB 1 cut(s) 599
PstI CTGCAG 3 cut(s) 1201, 1302, 1444
PstNI CAGNNNCTG 2 cut(s) 764, 1205
PsuI RGATCY 3 cut(s) 1137, 1476, 1772
PvuI CGATCG 1 cut(s) 2445
PvuII CAGCTG 1 cut(s) 698
RsaI GTAC 2 cut(s) 1434, 1491
RsaNI GTAC 2 cut(s) 1433, 1490
RseI CAYNNNNRTG 8 cut(s) 120, 777, 1058, 1746, 1812, 2148, 2184, 2531
SacI GAGCTC 1 cut(s) 1292
SatI GCNGC 9 cut(s) 942, 1197, 1298, 1443, 1896, 1956, 2308, 2439, 2649
Sau3AI GATC 9 cut(s) 178, 1137, 1476, 1512, 1772, 2266, 2442, 2539, 2743
Sau96I GGNCC 6 cut(s) 28, 535, 2014, 2164, 2200, 2729
SchI GAGTC 8 cut(s) 293, 524, 830, 874, 1562, 1737, 2057, 2187
SduI GDGCHC 6 cut(s) 600, 774, 1055, 1292, 1743, 1936
SfaNI GCATC 6 cut(s) 146, 1662, 1905, 2418, 2624, 2690
SfcI CTRYAG 3 cut(s) 1197, 1298, 1440
SfoI GGCGCC 1 cut(s) 986
Sfr274I CTCGAG 2 cut(s) 400, 599
SfuI TTCGAA 1 cut(s) 734
SinI GGWCC 2 cut(s) 2014, 2164
SlaI CTCGAG 2 cut(s) 400, 599
SmiMI CAYNNNNRTG 8 cut(s) 120, 777, 1058, 1746, 1812, 2148, 2184, 2531
SmlI CTYRAG 8 cut(s) 149, 259, 400, 599, 1256, 1308, 1732, 2674
SmoI CTYRAG 8 cut(s) 149, 259, 400, 599, 1256, 1308, 1732, 2674
SsiI CCGC 4 cut(s) 347, 1574, 1912, 2356
SspDI GGCGCC 1 cut(s) 984
SspI AATATT 1 cut(s) 2404
SspMI CTAG 4 cut(s) 795, 1362, 1485, 1929
SstI GAGCTC 1 cut(s) 1292
TaaI ACNGT 4 cut(s) 166, 905, 1432, 2337
TaiI ACGT 3 cut(s) 1750, 1788, 2284
TaqI TCGA 8 cut(s) 281, 314, 401, 600, 734, 1010, 2445, 2632
TatI WGTACW 1 cut(s) 1432
TfiI GAWTC 5 cut(s) 1012, 1076, 1202, 1523, 2039
TscAI CASTG 3 cut(s) 625, 757, 1681
TseFI GTSAC 1 cut(s) 129
TseI GCWGC 9 cut(s) 941, 1196, 1297, 1442, 1895, 1955, 2307, 2438, 2648
Tsp45I GTSAC 1 cut(s) 129
TspGWI ACGGA 2 cut(s) 882, 1130
TspRI CASTG 3 cut(s) 625, 757, 1681
Vha464I CTTAAG 1 cut(s) 259
VpaK11BI GGWCC 2 cut(s) 2014, 2164
XapI RAATTY 7 cut(s) 274, 1178, 1580, 1887, 2117, 2257, 2634
XceI RCATGY 4 cut(s) 106, 119, 1465, 2137
XcmI CCANNNNNNNNNTGG 1 cut(s) 1854
XhoI CTCGAG 2 cut(s) 400, 599
XmiI GTMKAC 1 cut(s) 2417
XmnI GAANNNNTTC 1 cut(s) 1463
XspI CTAG 4 cut(s) 795, 1362, 1485, 1929
Zsp2I ATGCAT 3 cut(s) 1819, 1920, 2524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.