Rorug04G0230500

Protein MEI2-like 4 isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0001846
Physical Location & Seq
Reverse (-)
39514736 .. 39521747
7012 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0230500.1

Sequence Viewer

Length: 4239 bp
ATGTTTGAGCTCTCTCTCCTTACCTCCGCTGCCGGAGGAGTAGTGAGTAAGGTGGCTTCACTTGCTACTGATCAATTGAGACTCGAAATCGTCTGCTGTTTCCGCAAAGAGCTAAATAAGCTCAAAGAATCCTCAGATCAGATTGGGGATATGATGCATGATGCTGCGCACGAACCAGAAGATCGGAAACTGCGGGCAAGGGCCAGGTGGATGCAGAGGCTTATAAGCGTAGCTCATGCTGCAGAAGATGTCCTGGATGAAGTTCAATATGAAGTAGATCGGATCGTTATAGAGGAGACAAGCCTGGATAAGAAGATACTCGGCTTCTTCTGCAACAATCCTGTTGTATTTCGTCTTCAGATGGCATGCAAGATTCACAACATCAACACATCCTTGGAGAATCTAAAAAAGGATGCACCCGCTGTGGGACTCGTCATAACAATGGGATCAAATGGAGGTGCAGCAGCAAGTCAACGTAGGCGGGACAGGGAAACAACGGCATTCCCAGAGATAATTGAATTCACCAGGAAAGATGCAAAAATTGTTGGAAGGGATCAGGCTGTGTTAGATATAATTGCAACCTTGACCACCTCCCACAATCAAGAAAATATTCCTTCGGTGATGGCTATCGTAGGATTGGGAGGCCTAGGTAAAACAACTTTGGCTCGCTCAGTTACCAACAAACTGAAAGAAGGTGAAATGAAAAAGTACTTTGATACAACGTTTTGGGTGTATGTATCTAATATTTTTGATGTCAACTCAATTTTACATCGAATGGTGCAATCATGTGGCGTGACGGGAATAGACTCTTCAAATCAGCAAGCATTGATGGAAAGCCTCCAGCAGAAGTTGAAAGACAAAAGGTATTTTCTTGTACTTGATGACGTTTGGAATGAGGAAGGTGATAATTGGAGTAAGTTGATGGGTTGTTTGTCACAAGTAAATTCTGCCAAAGGAAGCTCTGTGATTGTCACCACTCGTAGATCCAATGTTGCAACAATCATGGAAACACTTCCTAGGTGGAATTTAAAAACTTTATCGGATGATGATTGTTGGTCCATATTGGAGGATAGAGCATTTGTAGGTCCTAATGCTCCCATAGCTTCAGAATCACTAAAGGAAATCGGAAGGGACATTGCCAAAAAGTGTGCTGGTCTTCCATTGGTGGCAAAGGTTTTGGGAAGCTTGATGCGTTCTAAGAACAGTAGCGACGAATGGTTGTCAATTCTGGAAAGTAAAATATGGCAGGTATCATCAGGAAAAGACGATAGCAGGATCATGTCAGTTCTGAGGTTGAGTTTTGACAATTTGGAATCACCATTGAAACAATGTTTTGCATATTGCTCGATGCTCAAAAGAGGTTCCTCAATTGAAAGAGATAACCTGATCCAACTGTGGATGGCTCAAGGTTTTCTCCGTCCTTCTCATGATCTCCAAAGCCAGGGGATTGAAATGGAGGATATAGGCAGCAGGTATTTTGATACTCTACTGGAGAGCTCCTTGTTTCAAGATGCTAAAAAGGATGAGGATGGCGTTATCACCGAATCCAAGATGCACGATCTTGTGCATGATCTTGCAATAGAAGTATCCAAGTGTGAGAGCTTGACACCAGACCTCAATCATGAGATGGATGATCATGAGATTCGACATGTTGCAATGATTCTGACTTCAGAACTAAAAAGGATTCCAGAAACAAGTATTTCAAGACTACGTTCACTCTTTTCAAGTGTCAAGGTCCTTAATATCATTTTGCCAAGGTTGAGAGCTTTACGGGTCTTAAGTTTCTGTGAGGTAGAGAACCAGGAGTCGCCAAATTCACTTAGCAAGTTGAAGCACTTGAGGTATCTAGATCTTTCCCAAACAGAAATCCCAAAGTCTATTGGCAAGCTCTATAACCTCCAAACATTGAGATTACCACTGTATCTTGAAAAATGTCCAAAGGAGATTCAAAATTTGATTAACTTGAGGCATCTTTATCCACCAATGGAATTTGATGCTGGAATTGTGGGGCGATTAACTAAAATCCGAACATTACCCTGTTTTAATGTGGGTAAGCGGACCACGTTACCCTGTTTTAATGTGGGTAAGGGGACTGGTCCTGCAATCGAGGAGTTGGGTGGGTTGAAGCATTTGAGAGGCCACTTACATATTTGGAAGCTAGAACATGTGAGGGATGGAGAAGAAGCAAAGAATGCTTGCCTGGCGGAGAAGAGTCACTTGAGCGAGTTAACATGTGACTGGACAGAGGATGAGGTACCTAATGACAACGAGACGGATGTACTAGATGGCTTGAAACCTCATGGTAACTTGCAAAGCTTGAACATTTACGGTTTCAAGGGTGCTAGACTTCCGTCATGGATAACGGTTCTCCAAAATTTGAAGAAGATTCGATTATATGGCTGTAACAATTGTGAAGGAATCCCAACACTCGGCCATCTACCTAATTTAAGATCTCTTGATATTAAGATGCATAAGCTGATACGTGTGGGAGCTGAGTTTTATGGCACTGGTACAACTTCATTTCGAGCACTGAAAACATTATACATCAAGGAGTGCCAAGAGCTCATTGAATGGATGGAAGCACCGAGGATATCAGCTGAAGGAGAAGTTGTGGTATTTCCTTGCCTCGAAGAGTTGGATCTCAGGTGCTGTCGTAAATTGAGAAATGCTCCGAGTCGTTTTCCACGTCTCAAGATGTTGGTGATGGATAGAATGGGTAACAGCATGCCAATAGAAAATATAAGCACTCAACTTACCACTCTCACTTATCTCACAATAATTGGTGTAAAGGGACTCACTTGTCTACCAGAAGGGATGCTGAACAATAACAATAGTCTCACACATTTAGAGGTAAGTCGTTGTGATGAGTTGACGTGTATTGCTCTTGATGTATCTGACACGCTCCCTTTTCTCGAGGAGTTGACGATAAAGCGGTGTCCCAGCCTAGAGTCGATTCGGATCACACGGGGCATTGCATCTCTCCGTCAATTGATGATCTACAATTGTGAGGGATTATCAAGCCTAGAGGTTGGGCTTGATTACTGCACCTCTCTTCTCGAGGAGTTGAGGATATCTGAATGTGACAGCCTAGAGTCGATTCGGATCACACAGGGCATCGCATCTCTCCGTCAATTGAACATCAGGAGCTGTAAGGGATTATCAAGCCTAGAGGTTGGGCTCGATTACTGCACCTCTCTTCAGGAGTTGGATATAAGTTATTGCCCTAATCTGGTTTCAATTCCAACCGCACAGGGCATGCCATCTCTCCAGATATTGCGGATCTGGGCTTGTGAGGGATTATCAAGCATAGGAAGTGGGCTCAACTACTGCACCTCTCTTCAGGAATTGGAGATTGGGCACTGTTATAATCTGACATCAATTCCAATCACACAGGGCATCACATCTCTCCTGAGCTTGAGGATTCTTTATTGTCAGAGATTATTAAGCCTGCCGAGTGGGCTCCAATTCTGCACCTCTCTTCAGAAATTGGATATTGGTCCGTTGTGGGAGGAGCTGGACGCCTTCCCTGATTTTCAGCTTTCACCACCACATGATTCACAAAATTTGATCGGACTAGAAGCTCTTCCTGAGTGGTTGGGAAATCTTACATCTCTTGAGGCTCTGTGGATCTGGGAGTGTAAGAATCTCAAGTATCTACCTACACAAAATGTTATGACAAACCTCACCAAATTGAAGTCTCTCGTTGCTATAGGATGTCCCCTGCTCGAAGAAAGATGCACCAAGGAGACTGGCCCAGAATGGCCCAAGATCTCTCACATCCCAGATGTGTTAATCAGCATCAGCTATGAGAATGAGAAATTGAGAAGTATCCTCCTAAAGTGGAAGGTGGCTGCTGTTCGTTTTTCTCTCGCAAAGGCCGGTAGCTTTGCCCTATATTCAGAGCAAACTCCATCTGCATCTTGGATAAGCCATTTAGTCAAGCATGCATGGCTTCCAGGAAGCAAGCCTTCTATTATCTACGAGCCGTATGAAGAACCTCCTGTCAGATTTTGCATTTTTGTGCTCTCTTGGTGTTTCCATGAAAGCTTAACGAATGGAGCAACAAACAAGTCCTGGAGGACCTGGACAATTTGGTACGGTGGGCTTGTTTATGAGTTACTCATATATGGTTGTGGAGCTTATTGTGAGCTTCTCGGCACTTGTCATACTTTCAAGTATCAAGCCATATTTCTGAGATATGAGCAATTATCCAGGCAAAAATGGCATCAGTTGTGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1 RRM_2

Protein Analysis

1412

Amino Acids

159.21

Weight (kDa)

6.19

Isoelectric Point (pI)

49.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rx_N PF18052 13 - 94 3.1e-08 Rx N-terminal domain
NB-ARC PF00931 188 - 360 2.6e-33 NB-ARC domain
AAA_22 PF13401 206 - 307 2.4e-07 AAA domain
NACHT PF05729 207 - 348 1.3e-07 NACHT domain
WHD_DRP PF23559 453 - 526 1.2e-20 Disease resistance protein Winged helix domain
LRR_14 PF23598 570 - 852 1.9e-29 Leucine-rich repeat region
LRR_R13L1-DRL21 PF25019 702 - 822 1.3e-36 R13L1/DRL21 LRRs
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 224, 3366
AasI GACNNNNNNGTC 1 cut(s) 2799
Acc16I TGCGCA 1 cut(s) 168
Acc36I ACCTGC 2 cut(s) 1237, 1461
Acc65I GGTACC 1 cut(s) 2254
AccB1I GGYRCC 1 cut(s) 2254
AccI GTMKAC 1 cut(s) 2803
AclI AACGTT 1 cut(s) 722
AcoI YGGCCR 1 cut(s) 2431
AcsI RAATTY 8 cut(s) 518, 943, 1024, 1813, 1951, 1988, 2374, 3562
AcuI CTGAAG 7 cut(s) 341, 1089, 1653, 2619, 3182, 3323, 3464
AcyI GRCGYC 1 cut(s) 3519
AfaI GTAC 6 cut(s) 710, 876, 2256, 2280, 2512, 4097
AfiI CCNNNNNNNGG 4 cut(s) 425, 1441, 2429, 3229
AflII CTTAAG 1 cut(s) 1777
AflIII ACRYGT 5 cut(s) 1648, 2164, 2231, 2482, 2873
AjiI CACGTC 2 cut(s) 2687, 2874
AjuI GAANNNNNNNTTGG 2 cut(s) 3336, 3368
Alw21I GWGCWC 5 cut(s) 12, 1499, 2530, 2565, 4026
Alw26I GTCTC 7 cut(s) 73, 290, 2264, 2693, 2840, 3702, 3740
AlwNI CAGNNNCTG 2 cut(s) 2649, 3147
Ama87I CYCGRG 2 cut(s) 2912, 3056
AoxI GGCC 7 cut(s) 201, 643, 2137, 2431, 3751, 3761, 3876
ApeKI GCWGC 7 cut(s) 29, 164, 239, 461, 464, 1467, 3851
ApoI RAATTY 8 cut(s) 518, 943, 1024, 1813, 1951, 1988, 2374, 3562
Asp700I GAANNNNTTC 3 cut(s) 609, 1011, 3582
Asp718I GGTACC 1 cut(s) 2254
AspA2I CCTAGG 2 cut(s) 646, 1016
AspLEI GCGC 1 cut(s) 169
AvaI CYCGRG 2 cut(s) 2912, 3056
AvaII GGWCC 7 cut(s) 1056, 1085, 1735, 2058, 2096, 3497, 4080
AvrII CCTAGG 2 cut(s) 646, 1016
BaeGI GKGCMC 1 cut(s) 3360
BaeI ACNNNNGTAYC 2 cut(s) 1904, 1937
BanI GGYRCC 1 cut(s) 2254
BanII GRGCYC 6 cut(s) 12, 1499, 2565, 3180, 3321, 3462
BarI GAAGNNNNNNTAC 4 cut(s) 40, 72, 339, 371
BbsI GAAGAC 2 cut(s) 347, 1148
Bbv12I GWGCWC 5 cut(s) 12, 1499, 2530, 2565, 4026
BbvI GCAGC 7 cut(s) 16, 151, 226, 473, 476, 1479, 3838
BceAI ACGGC 2 cut(s) 513, 3970
BcgI CGANNNNNNTGC 2 cut(s) 1326, 1360
BciVI GTATCC 2 cut(s) 1597, 3839
BclI TGATCA 2 cut(s) 70, 1633
BcoDI GTCTC 7 cut(s) 73, 290, 2264, 2693, 2840, 3702, 3740
BfmI CTRYAG 2 cut(s) 240, 3708
BfrI CTTAAG 1 cut(s) 1777
BfuAI ACCTGC 2 cut(s) 1237, 1461
BfuI GTATCC 2 cut(s) 1597, 3839
BglI GCCNNNNNGGC 1 cut(s) 3457
BglII AGATCT 3 cut(s) 1849, 2450, 3768
BisI GCNGC 7 cut(s) 30, 165, 240, 462, 465, 1468, 3852
BlnI CCTAGG 2 cut(s) 646, 1016
BlsI GCNGC 7 cut(s) 31, 166, 241, 463, 466, 1469, 3853
BmcAI AGTACT 1 cut(s) 710
Bme18I GGWCC 7 cut(s) 1056, 1085, 1735, 2058, 2096, 3497, 4080
BmeT110I CYCGRG 2 cut(s) 2912, 3056
BmgBI CACGTC 2 cut(s) 2687, 2874
BmiI GGNNCC 3 cut(s) 1363, 2256, 3461
BoxI GACNNNNGTC 1 cut(s) 2350
BpiI GAAGAC 2 cut(s) 347, 1148
BplI GAGNNNNNCTC 2 cut(s) 2653, 2685
BpmI CTGGAG 4 cut(s) 824, 1511, 3251, 4096
Bpu10I CCTNAGC 1 cut(s) 3410
BpuEI CTTGAG 8 cut(s) 1389, 1858, 1984, 2239, 2675, 3436, 3632, 3635
BsaAI YACGTR 1 cut(s) 2483
BsaBI GATNNNNATC 3 cut(s) 626, 2957, 3101
BsaHI GRCGYC 1 cut(s) 3519
BsaJI CCNNGG 7 cut(s) 393, 646, 1016, 1441, 1754, 2585, 3741
BsaXI ACNNNNNCTCC 4 cut(s) 27, 57, 2595, 2625
Bsc4I CCNNNNNNNGG 4 cut(s) 425, 1441, 2429, 3229
Bse118I RCCGGY 1 cut(s) 3878
Bse1I ACTGG 5 cut(s) 1494, 2098, 2243, 2512, 3754
Bse3DI GCAATG 3 cut(s) 1134, 1662, 2970
Bse8I GATNNNNATC 3 cut(s) 626, 2957, 3101
BseDI CCNNGG 7 cut(s) 393, 646, 1016, 1441, 1754, 2585, 3741
BseJI GATNNNNATC 3 cut(s) 626, 2957, 3101
BseLI CCNNNNNNNGG 4 cut(s) 425, 1441, 2429, 3229
BseMI GCAATG 3 cut(s) 1134, 1662, 2970
BseMII CTCAG 8 cut(s) 147, 684, 1280, 2484, 2656, 3401, 3579, 4184
BseNI ACTGG 5 cut(s) 1494, 2098, 2243, 2512, 3754
BseRI GAGGAG 6 cut(s) 51, 308, 2123, 2930, 3074, 3524
BseSI GKGCMC 1 cut(s) 3360
BseXI GCAGC 7 cut(s) 16, 151, 226, 473, 476, 1479, 3838
BseYI CCCAGC 1 cut(s) 2939
BsgI GTGCAG 5 cut(s) 480, 3028, 3172, 3313, 3454
BshFI GGCC 7 cut(s) 203, 645, 2139, 2433, 3753, 3763, 3878
BshNI GGYRCC 1 cut(s) 2254
BsiHKAI GWGCWC 5 cut(s) 12, 1499, 2530, 2565, 4026
BsiHKCI CYCGRG 2 cut(s) 2912, 3056
BsiSI CCGG 2 cut(s) 33, 3879
BslFI GGGAC 7 cut(s) 441, 497, 1145, 2104, 2805, 2922, 3702
BslI CCNNNNNNNGG 4 cut(s) 425, 1441, 2429, 3229
BsmAI GTCTC 7 cut(s) 73, 290, 2264, 2693, 2840, 3702, 3740
BsmBI CGTCTC 2 cut(s) 2264, 2693
BsmFI GGGAC 7 cut(s) 441, 497, 1145, 2104, 2805, 2922, 3702
BsmI GAATGC 2 cut(s) 500, 2197
BsnI GGCC 7 cut(s) 203, 645, 2139, 2433, 3753, 3763, 3878
BsoBI CYCGRG 2 cut(s) 2912, 3056
Bsp1286I GDGCHC 9 cut(s) 12, 1499, 2530, 2565, 3180, 3321, 3360, 3462, 4026
BspANI GGCC 7 cut(s) 203, 645, 2139, 2433, 3753, 3763, 3878
BspCNI CTCAG 8 cut(s) 146, 683, 1281, 2485, 2655, 3402, 3580, 4185
BspHI TCATGA 3 cut(s) 1426, 1621, 1636
BspLI GGNNCC 3 cut(s) 1363, 2256, 3461
BspMAI CTGCAG 1 cut(s) 244
BspMI ACCTGC 2 cut(s) 1237, 1461
BspQI GCTCTTC 1 cut(s) 3588
BspT107I GGYRCC 1 cut(s) 2254
BspTI CTTAAG 1 cut(s) 1777
BsrDI GCAATG 3 cut(s) 1134, 1662, 2970
BsrFI RCCGGY 1 cut(s) 3878
BsrI ACTGG 5 cut(s) 1494, 2098, 2243, 2512, 3754
BssAI RCCGGY 1 cut(s) 3878
BssECI CCNNGG 7 cut(s) 393, 646, 1016, 1441, 1754, 2585, 3741
BssNI GRCGYC 1 cut(s) 3519
BssT1I CCWWGG 5 cut(s) 393, 646, 1016, 1754, 3741
Bst4CI ACNGT 7 cut(s) 1205, 1395, 1920, 2330, 2365, 3362, 4100
Bst6I CTCTTC 8 cut(s) 814, 2204, 2625, 3057, 3201, 3342, 3483, 3588
BstACI GRCGYC 1 cut(s) 3519
BstAFI CTTAAG 1 cut(s) 1777
BstAPI GCANNNNNTGC 1 cut(s) 2192
BstBAI YACGTR 1 cut(s) 2483
BstHHI GCGC 1 cut(s) 169
BstMAI GTCTC 7 cut(s) 73, 290, 2264, 2693, 2840, 3702, 3740
BstNSI RCATGY 7 cut(s) 369, 1652, 2168, 2235, 2728, 3259, 3947
BstPAI GACNNNNGTC 1 cut(s) 2350
BstSFI CTRYAG 2 cut(s) 240, 3708
BstSLI GKGCMC 1 cut(s) 3360
BstV1I GCAGC 7 cut(s) 16, 151, 226, 473, 476, 1479, 3838
BstV2I GAAGAC 2 cut(s) 347, 1148
BstX2I RGATCY 7 cut(s) 983, 1849, 2450, 2638, 3279, 3627, 3768
BstYI RGATCY 7 cut(s) 983, 1849, 2450, 2638, 3279, 3627, 3768
BsuI GTATCC 2 cut(s) 1597, 3839
BsuRI GGCC 7 cut(s) 203, 645, 2139, 2433, 3753, 3763, 3878
BtgZI GCGATG 1 cut(s) 3100
BtrI CACGTC 2 cut(s) 2687, 2874
BtsIMutI CAGTG 4 cut(s) 1916, 2505, 2528, 3358
BveI ACCTGC 2 cut(s) 1237, 1461
CaiI CAGNNNCTG 2 cut(s) 2649, 3147
CciI TCATGA 3 cut(s) 1426, 1621, 1636
CfoI GCGC 1 cut(s) 169
Cfr10I RCCGGY 1 cut(s) 3878
CseI GACGC 1 cut(s) 3527
Csp6I GTAC 6 cut(s) 709, 875, 2255, 2279, 2511, 4096
CviQI GTAC 6 cut(s) 709, 875, 2255, 2279, 2511, 4096
DraI TTTAAA 1 cut(s) 1029
DrdI GACNNNNNNGTC 1 cut(s) 2799
DseDI GACNNNNNNGTC 1 cut(s) 2799
EaeI YGGCCR 1 cut(s) 2431
Eam1104I CTCTTC 8 cut(s) 814, 2204, 2625, 3057, 3201, 3342, 3483, 3588
EarI CTCTTC 8 cut(s) 814, 2204, 2625, 3057, 3201, 3342, 3483, 3588
EciI GGCGGA 1 cut(s) 2219
Ecl136II GAGCTC 3 cut(s) 10, 1497, 2563
Eco130I CCWWGG 5 cut(s) 393, 646, 1016, 1754, 3741
Eco147I AGGCCT 1 cut(s) 645
Eco24I GRGCYC 6 cut(s) 12, 1499, 2565, 3180, 3321, 3462
Eco32I GATATC 2 cut(s) 2592, 3072
Eco47I GGWCC 7 cut(s) 1056, 1085, 1735, 2058, 2096, 3497, 4080
Eco53kI GAGCTC 3 cut(s) 10, 1497, 2563
Eco57I CTGAAG 7 cut(s) 341, 1089, 1653, 2619, 3182, 3323, 3464
Eco88I CYCGRG 2 cut(s) 2912, 3056
EcoICRI GAGCTC 3 cut(s) 10, 1497, 2563
EcoO109I RGGNCCY 3 cut(s) 1085, 1735, 4080
EcoRI GAATTC 1 cut(s) 518
EcoRV GATATC 2 cut(s) 2592, 3072
EcoT14I CCWWGG 5 cut(s) 393, 646, 1016, 1754, 3741
EcoT22I ATGCAT 3 cut(s) 159, 2472, 3949
EcoT38I GRGCYC 6 cut(s) 12, 1499, 2565, 3180, 3321, 3462
ErhI CCWWGG 5 cut(s) 393, 646, 1016, 1754, 3741
Esp3I CGTCTC 2 cut(s) 2264, 2693
FalI AAGNNNNNCTT 4 cut(s) 2201, 2233, 3952, 3984
FaqI GGGAC 7 cut(s) 441, 497, 1145, 2104, 2805, 2922, 3702
FauI CCCGC 3 cut(s) 186, 427, 474
FbaI TGATCA 2 cut(s) 70, 1633
FblI GTMKAC 1 cut(s) 2803
Fnu4HI GCNGC 7 cut(s) 30, 165, 240, 462, 465, 1468, 3852
FriOI GRGCYC 6 cut(s) 12, 1499, 2565, 3180, 3321, 3462
Fsp4HI GCNGC 7 cut(s) 30, 165, 240, 462, 465, 1468, 3852
FspI TGCGCA 1 cut(s) 168
GlaI GCGC 1 cut(s) 168
GluI GCNGC 7 cut(s) 30, 165, 240, 462, 465, 1468, 3852
GsaI CCCAGC 1 cut(s) 2943
GsuI CTGGAG 4 cut(s) 824, 1511, 3251, 4096
HaeIII GGCC 7 cut(s) 203, 645, 2139, 2433, 3753, 3763, 3878
HapII CCGG 2 cut(s) 33, 3879
HgaI GACGC 1 cut(s) 3527
HhaI GCGC 1 cut(s) 169
Hin1I GRCGYC 1 cut(s) 3519
Hin6I GCGC 1 cut(s) 167
HinP1I GCGC 1 cut(s) 167
HincII GTYRAC 5 cut(s) 473, 757, 2229, 2871, 2922
HindII GTYRAC 5 cut(s) 473, 757, 2229, 2871, 2922
HindIII AAGCTT 3 cut(s) 1183, 2314, 4045
HpaI GTTAAC 1 cut(s) 2229
HpaII CCGG 2 cut(s) 33, 3879
Hpy166II GTNNAC 7 cut(s) 473, 757, 1715, 2229, 2804, 2871, 2922
Hpy8I GTNNAC 7 cut(s) 473, 757, 1715, 2229, 2804, 2871, 2922
Hpy99I CGWCG 1 cut(s) 1214
HpyCH4III ACNGT 7 cut(s) 1205, 1395, 1920, 2330, 2365, 3362, 4100
HpyCH4IV ACGT 8 cut(s) 475, 722, 885, 1711, 2063, 2482, 2686, 2873
HpySE526I ACGT 8 cut(s) 475, 722, 885, 1711, 2063, 2482, 2686, 2873
Hsp92I GRCGYC 1 cut(s) 3519
HspAI GCGC 1 cut(s) 167
KpnI GGTACC 1 cut(s) 2258
Ksp22I TGATCA 2 cut(s) 70, 1633
KspAI GTTAAC 1 cut(s) 2229
LguI GCTCTTC 1 cut(s) 3588
Lsp1109I GCAGC 7 cut(s) 16, 151, 226, 473, 476, 1479, 3838
MaeII ACGT 8 cut(s) 475, 722, 885, 1711, 2063, 2482, 2686, 2873
MfeI CAATTG 6 cut(s) 74, 1368, 2407, 2987, 3001, 3131
MflI RGATCY 7 cut(s) 983, 1849, 2450, 2638, 3279, 3627, 3768
MhlI GDGCHC 9 cut(s) 12, 1499, 2530, 2565, 3180, 3321, 3360, 3462, 4026
MlyI GAGTC 9 cut(s) 75, 423, 800, 1814, 2221, 2683, 2787, 2957, 3101
MmeI TCCRAC 5 cut(s) 526, 1414, 2616, 3186, 3266
Mph1103I ATGCAT 3 cut(s) 159, 2472, 3949
MroXI GAANNNNTTC 3 cut(s) 609, 1011, 3582
MslI CAYNNNNRTG 2 cut(s) 440, 4019
MspA1I CMGCKG 3 cut(s) 29, 422, 2597
MspCI CTTAAG 1 cut(s) 1777
MspI CCGG 2 cut(s) 33, 3879
MunI CAATTG 6 cut(s) 74, 1368, 2407, 2987, 3001, 3131
Mva1269I GAATGC 2 cut(s) 500, 2197
NlaIV GGNNCC 3 cut(s) 1363, 2256, 3461
NmeAIII GCCGAG 4 cut(s) 300, 2409, 3477, 4134
NmuCI GTSAC 6 cut(s) 793, 933, 970, 2213, 2234, 3080
NsbI TGCGCA 1 cut(s) 168
NsiI ATGCAT 3 cut(s) 159, 2472, 3949
NspI RCATGY 7 cut(s) 369, 1652, 2168, 2235, 2728, 3259, 3947
PaeI GCATGC 4 cut(s) 369, 2728, 3259, 3947
PaeR7I CTCGAG 2 cut(s) 2912, 3056
PagI TCATGA 3 cut(s) 1426, 1621, 1636
PceI AGGCCT 1 cut(s) 645
PciI ACATGT 3 cut(s) 1648, 2164, 2231
PciSI GCTCTTC 1 cut(s) 3588
PcsI WCGNNNNNNNCGW 1 cut(s) 2683
PctI GAATGC 2 cut(s) 500, 2197
PdmI GAANNNNTTC 3 cut(s) 609, 1011, 3582
PfoI TCCNGGA 3 cut(s) 252, 3955, 4073
PkrI GCNGC 7 cut(s) 31, 166, 241, 463, 466, 1469, 3853
PleI GAGTC 9 cut(s) 75, 423, 800, 1813, 2220, 2682, 2787, 2956, 3100
PpsI GAGTC 9 cut(s) 75, 423, 800, 1813, 2220, 2682, 2787, 2956, 3100
Ppu21I YACGTR 1 cut(s) 2483
PpuMI RGGWCCY 3 cut(s) 1085, 1735, 4080
PscI ACATGT 3 cut(s) 1648, 2164, 2231
PshAI GACNNNNGTC 1 cut(s) 2350
PsiI TTATAA 2 cut(s) 224, 3366
Psp124BI GAGCTC 3 cut(s) 12, 1499, 2565
Psp1406I AACGTT 1 cut(s) 722
Psp5II RGGWCCY 3 cut(s) 1085, 1735, 4080
PspFI CCCAGC 1 cut(s) 2939
PspN4I GGNNCC 3 cut(s) 1363, 2256, 3461
PspPPI RGGWCCY 3 cut(s) 1085, 1735, 4080
PstI CTGCAG 1 cut(s) 244
PstNI CAGNNNCTG 2 cut(s) 2649, 3147
PsuI RGATCY 7 cut(s) 983, 1849, 2450, 2638, 3279, 3627, 3768
PvuII CAGCTG 1 cut(s) 2597
RsaI GTAC 6 cut(s) 710, 876, 2256, 2280, 2512, 4097
RsaNI GTAC 6 cut(s) 709, 875, 2255, 2279, 2511, 4096
RseI CAYNNNNRTG 2 cut(s) 440, 4019
SacI GAGCTC 3 cut(s) 12, 1499, 2565
SapI GCTCTTC 1 cut(s) 3588
SatI GCNGC 7 cut(s) 30, 165, 240, 462, 465, 1468, 3852
ScaI AGTACT 1 cut(s) 710
SchI GAGTC 9 cut(s) 75, 423, 800, 1814, 2221, 2683, 2787, 2957, 3101
SduI GDGCHC 9 cut(s) 12, 1499, 2530, 2565, 3180, 3321, 3360, 3462, 4026
SfcI CTRYAG 2 cut(s) 240, 3708
Sfr274I CTCGAG 2 cut(s) 2912, 3056
SinI GGWCC 7 cut(s) 1056, 1085, 1735, 2058, 2096, 3497, 4080
SlaI CTCGAG 2 cut(s) 2912, 3056
SmiMI CAYNNNNRTG 2 cut(s) 440, 4019
SphI GCATGC 4 cut(s) 369, 2728, 3259, 3947
SseBI AGGCCT 1 cut(s) 645
SspI AATATT 2 cut(s) 610, 745
SstI GAGCTC 3 cut(s) 12, 1499, 2565
StuI AGGCCT 1 cut(s) 645
StyI CCWWGG 5 cut(s) 393, 646, 1016, 1754, 3741
TaaI ACNGT 7 cut(s) 1205, 1395, 1920, 2330, 2365, 3362, 4100
TaiI ACGT 8 cut(s) 478, 725, 888, 1714, 2066, 2485, 2689, 2876
TatI WGTACW 3 cut(s) 708, 874, 2278
TscAI CASTG 4 cut(s) 1923, 2512, 2535, 3365
TseFI GTSAC 6 cut(s) 793, 933, 970, 2213, 2234, 3080
TseI GCWGC 7 cut(s) 29, 164, 239, 461, 464, 1467, 3851
Tsp45I GTSAC 6 cut(s) 793, 933, 970, 2213, 2234, 3080
TspDTI ATGAA 6 cut(s) 273, 285, 716, 2508, 4005, 4056
TspGWI ACGGA 6 cut(s) 1406, 2288, 2340, 2972, 3116, 3489
TspRI CASTG 4 cut(s) 1923, 2512, 2535, 3365
Vha464I CTTAAG 1 cut(s) 1777
VpaK11BI GGWCC 7 cut(s) 1056, 1085, 1735, 2058, 2096, 3497, 4080
XapI RAATTY 8 cut(s) 518, 943, 1024, 1813, 1951, 1988, 2374, 3562
XbaI TCTAGA 1 cut(s) 1846
XceI RCATGY 7 cut(s) 369, 1652, 2168, 2235, 2728, 3259, 3947
XcmI CCANNNNNNNNNTGG 1 cut(s) 3918
XhoI CTCGAG 2 cut(s) 2912, 3056
XmaJI CCTAGG 2 cut(s) 646, 1016
XmiI GTMKAC 1 cut(s) 2803
XmnI GAANNNNTTC 3 cut(s) 609, 1011, 3582
ZrmI AGTACT 1 cut(s) 710
Zsp2I ATGCAT 3 cut(s) 159, 2472, 3949
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.