Rh4AG287000

Protein MEI2-like 4 isoform X1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0001846
Physical Location & Seq
Reverse (-)
60091934 .. 60099894
7961 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG287000.1

Sequence Viewer

Length: 2730 bp
ATGCCATCTGAAGTAATGGACATTAAGGGCCTGTCATCTTCTTCGTTCTTCTCCGAAGATTTCCCAAACGAGAGGCAGGTTGGGTTTTGGAAGTCAGATAACATGCCAGATAAACATGCCGGTGAGAAGTCACTTGCATCATCATCTCTTGAGAAGTGTCAGACGGTGAAATCTCTGGATCACCCTGATTTTTTCTTAATGCCAGAACAAAAGGTGAAACCCAGCTTCAATAGACAAGCAGTTGGGGCAGAGAGAGCCTTAAGTCATTCATTGAATTTATCGAGAGCCGTGAGCCTTGACTCGGGAGTAATATCTAATGTAAATGTTGAGACAGCATCTTACTATGCGGAAGGTGGCAAAGTCAATATGATGGGAACGCAGTATGAGAGTAGCCTTTTCTCGAGTTCATTATCTGAGTTGTTTAGTAGGAAGTTGAGACTATCGTCAAACAATACACAATATGGACATTCTGTTGATAGTGTTGCCTCAAACTATGAGGAAGAGGAAATTTTTGAGTCCCTTGAAGAAATGGAGGCCCAAACCATCGGAAACCTTCTCCCTGATGATGATGAGTTGCTTTCTGGAGTTACCGATGGGCTCGAGTATAATGTTCCACTCACTGCTGGGGATGATATTGAGGAGTTGGATTTTTTTAGCAGTAGTGGAGGGATGGATTTGGGAGACGATGGTTTGTCAGCTGGACTAAACAATTCTGATTGTCCTGGAGGAGTTTCGAATGGTCTGCAATGCAGTGGTTCAGTAGCTGGAGAACACCCTAATGGTGAACACCCTTCTAGGACATTGTTTGTGAGAAATATAAATAGTAACATTGAAGACTCTGAGTTGAGAACCCTGTTTGAGCAATACGGAGACATTCGGACTCTTTATACAGCCTGCAAACACCGTGGGTTTGTTATGATATCATATTATGATATTAGAGCAGCCCGGAATGCGATGAAAGCACTCCAGAACAAACCATTGAGGCGCCGGAAGCTTGACATTCATTACTCGATTCCAAAGGACAATCCTTCTGAGAAAGATGTTAATCAGGGCACTCTTGTGGTATTTAACCTTGATTCTTCTGTTTCTAATGATGAACTTCGTGAGCTTTTTGGTGTCTATGGGGAAATCAAAGAGATCCGTGAAACACCAAATAGAAGTCATCACAAGTTTATCGAATTTTATGATGTTAGAGCTGCAGAATCTGCTCTTAATGCCATGAACAGGAGTGATATAGCCGGGAAGAGGATTAAGCTTGAGCCAAGTCGTCCTGGAGGTGCAAAAAGGAGCTCGGGGCTACAGTTACCTCAAGAGTTAGAGCAGGATGATTGTGGCCTTTATTTTCAGCAGAGTAGTCCTCCTAGTAACTCTATTACTGGTTTCTCTGATAATGGAGCTATTATGACTGTACACTCTGCAGCTCCATCTTTTGAGAACATGTTCCATCATGGGATCTCATCTAGTGTACCCAACAGCCTATCTTCTATGATGAGAGTTGAATCAGCAGTCAGTCAGTCTGGCCATACTGAGTCCATTCATTCAGCATGCCCGCTGAAATTTGACATCCACGGCTCACCAGCTTTTCATCCTCATTCACTTCCAGAGTATCATAATGGTTTAACTAATGGCATCAACTGCAATTCAACAGGCAGTGTATCTGCAAGTATCAATGTCAGACCACCAGATAACAGGCACTTTACCGGAGTCTCAAGTGGGCACTCACGTGAACTTAATGATAGTGCTTTTGGATCTATGGGTAACGTGAACTGCCCTATTCCTGGACATCATTATGCATGGAACAATTCCTTTCAGTCTCAGGCTCCAGGAATGATGTGGCCAAACTCACCATCATTTGTTAATGGAATTTCGGCAGCCCATCCCCCACAGCGGATGCATGGACTTCCTAGAGCACCATCTCATATGTTAAACCCAGCAATGCCCATAAATAAACATCATGTGGGTTCAGCACCAGGGGCTAACCCAATGTGGGACCGGAGACAAGCATATGCAGGGGAATCCCCCGAGTCATCTGGATTTCATCCAGGCTCTCTTGGGAATATGAGAATGCCAAATAACTCACCTCATTCGTTGGAATTTGTTTCTCATAACATGTTTCCTCATGTTAATGGAAATGGCATGGAACTGCCAATTCCCCACAAAAATGCAGGACTCCAGGCCCATCATAAGAGGTGCATGATGTATCCTGGAAGAAGTCAGATGGTTCCTGTTATGAATTCATTTGATCAACCTACTGAACGTCCGAGAAGTCGTAGAAATGAAGGCAGCTCTAATCAGGTTGACAACAAGAAACAGTATGAACTTGATATTGACCGCATTATGCGAGGGGATGACAACAGAACAACACTTATGATAAAGAATATTCCGAACAAGTATACCTCAAAGATGCTTTTGGCTGCAATCGATGAGCGCCATCGTGGAACATATGATTTCATTTATCTGCCAATTGATTTTAAGAACAAATGCAATGTGGGGTATGCATTCATCAATATGACTGATCCGAGACAGATTGCTCCATTTTATCAGTCATTCAATGGGAAGAAATGGGAGAAGTTTAATAGTGAGAAGGTGGCATCAATTGCTTATGCTCGAATTCAAGGCAAAGCTGCTCTGATTGCACATTTCCAGAACTCAAGCCTGATGAATGAGGATAAGCGATGCCGTCCCATTCTCTTCAATACTGATGGCCCCAATGCAGGTGATCAGGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
RRM_1 RRM_2

Protein Analysis

909

Amino Acids

100.58

Weight (kDa)

6.08

Isoelectric Point (pI)

53.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_1 PF00076 268 - 332 1.6e-10 RNA recognition motif
RRM_5 PF13893 350 - 426 1.6e-06 RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
RRM_1 PF00076 353 - 418 1.2e-10 RNA recognition motif
RRM_2 PF04059 786 - 882 5.1e-53 RNA recognition motif 2
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2705
Acc36I ACCTGC 2 cut(s) 67, 2705
AccB1I GGYRCC 1 cut(s) 984
AccI GTMKAC 1 cut(s) 2393
AciI CCGC 4 cut(s) 347, 1550, 1888, 2332
AclWI GGATC 5 cut(s) 186, 1132, 1460, 1756, 2510
AcoI YGGCCR 2 cut(s) 1519, 1835
AcsI RAATTY 8 cut(s) 274, 507, 1178, 1556, 1863, 2093, 2233, 2610
AcuI CTGAAG 1 cut(s) 30
AcvI CACGTG 1 cut(s) 1724
AcyI GRCGYC 1 cut(s) 985
AfaI GTAC 2 cut(s) 1410, 1467
AfiI CCNNNNNNNGG 7 cut(s) 301, 1224, 1245, 1778, 1887, 1987, 2714
AflII CTTAAG 1 cut(s) 259
AflIII ACRYGT 2 cut(s) 1437, 2109
AgsI TTSAA 9 cut(s) 229, 274, 524, 833, 1499, 1644, 2551, 2615, 2695
AjnI CCWGG 8 cut(s) 721, 1270, 1777, 1822, 1969, 2041, 2172, 2203
AleI CACNNNNGTG 2 cut(s) 1058, 1722
Alw21I GWGCWC 2 cut(s) 1292, 1912
Alw26I GTCTC 8 cut(s) 323, 430, 675, 864, 1711, 1818, 1990, 2515
AlwI GGATC 5 cut(s) 186, 1132, 1460, 1756, 2510
AlwNI CAGNNNCTG 2 cut(s) 764, 1205
Ama87I CYCGRG 5 cut(s) 301, 400, 599, 1291, 2021
AoxI GGCC 7 cut(s) 28, 534, 1333, 1519, 1835, 2175, 2704
ApeKI GCWGC 7 cut(s) 941, 1196, 1418, 1871, 2283, 2414, 2624
ApoI RAATTY 8 cut(s) 274, 507, 1178, 1556, 1863, 2093, 2233, 2610
Asp700I GAANNNNTTC 1 cut(s) 1439
AspLEI GCGC 2 cut(s) 987, 2430
AspS9I GGNCC 5 cut(s) 28, 535, 1990, 2176, 2705
AsuC2I CCSGG 2 cut(s) 946, 1240
AsuHPI GGTGA 9 cut(s) 134, 173, 178, 226, 794, 1566, 1836, 2070, 2729
AsuII TTCGAA 1 cut(s) 734
AvaI CYCGRG 5 cut(s) 301, 400, 599, 1291, 2021
AvaII GGWCC 1 cut(s) 1990
BaeGI GKGCMC 2 cut(s) 1055, 1719
BalI TGGCCA 2 cut(s) 1521, 1837
BanI GGYRCC 1 cut(s) 984
BanII GRGCYC 2 cut(s) 600, 1292
BbrPI CACGTG 1 cut(s) 1724
BbsI GAAGAC 1 cut(s) 840
Bbv12I GWGCWC 2 cut(s) 1292, 1912
BbvI GCAGC 7 cut(s) 953, 1183, 1430, 1883, 2295, 2401, 2611
BceAI ACGGC 3 cut(s) 272, 1585, 2664
BcgI CGANNNNNNTGC 2 cut(s) 724, 758
BciT130I CCWGG 8 cut(s) 723, 1272, 1779, 1824, 1971, 2043, 2174, 2205
BciVI GTATCC 1 cut(s) 2211
BclI TGATCA 2 cut(s) 2242, 2719
BcnI CCSGG 2 cut(s) 946, 1240
BcoDI GTCTC 8 cut(s) 323, 430, 675, 864, 1711, 1818, 1990, 2515
BfaI CTAG 4 cut(s) 795, 1362, 1461, 1905
BfmI CTRYAG 3 cut(s) 1197, 1298, 1416
BfoI RGCGCY 2 cut(s) 988, 2431
BfrI CTTAAG 1 cut(s) 259
BfuAI ACCTGC 2 cut(s) 67, 2705
BfuI GTATCC 1 cut(s) 2211
BisI GCNGC 7 cut(s) 942, 1197, 1419, 1872, 2284, 2415, 2625
BlsI GCNGC 7 cut(s) 943, 1198, 1420, 1873, 2285, 2416, 2626
Bme18I GGWCC 1 cut(s) 1990
BmeT110I CYCGRG 5 cut(s) 301, 400, 599, 1291, 2021
BmgT120I GGNCC 5 cut(s) 28, 535, 1990, 2176, 2705
BmiI GGNNCC 5 cut(s) 986, 1821, 1991, 2223, 2707
BmsI GCATC 7 cut(s) 146, 344, 1638, 1881, 2394, 2600, 2666
BoxI GACNNNNGTC 1 cut(s) 442
BpiI GAAGAC 1 cut(s) 840
BplI GAGNNNNNCTC 2 cut(s) 1342, 1374
BpmI CTGGAG 7 cut(s) 603, 744, 786, 950, 1293, 1806, 2156
Bpu14I TTCGAA 1 cut(s) 734
BpuEI CTTGAG 5 cut(s) 170, 1277, 1293, 1693, 2635
BpuMI CCSGG 2 cut(s) 946, 1240
Bsa29I ATCGAT 1 cut(s) 2421
BsaAI YACGTR 1 cut(s) 1724
BsaBI GATNNNNATC 1 cut(s) 1044
BsaHI GRCGYC 1 cut(s) 985
BsaJI CCNNGG 3 cut(s) 904, 1567, 1970
BsaWI WCCGGW 2 cut(s) 1700, 1992
Bsc4I CCNNNNNNNGG 7 cut(s) 301, 1224, 1245, 1778, 1887, 1987, 2714
Bse118I RCCGGY 1 cut(s) 119
Bse1I ACTGG 1 cut(s) 1381
Bse3DI GCAATG 3 cut(s) 752, 1941, 2491
Bse8I GATNNNNATC 1 cut(s) 1044
BseBI CCWGG 8 cut(s) 723, 1272, 1779, 1824, 1971, 2043, 2174, 2205
BseCI ATCGAT 1 cut(s) 2421
BseDI CCNNGG 3 cut(s) 904, 1567, 1970
BseGI GGATG 9 cut(s) 634, 675, 1330, 1563, 1585, 1876, 1896, 2038, 2353
BseJI GATNNNNATC 1 cut(s) 1044
BseLI CCNNNNNNNGG 7 cut(s) 301, 1224, 1245, 1778, 1887, 1987, 2714
BseMI GCAATG 3 cut(s) 752, 1941, 2491
BseMII CTCAG 5 cut(s) 405, 831, 1023, 1518, 1829
BseNI ACTGG 1 cut(s) 1381
BseRI GAGGAG 2 cut(s) 653, 741
BseSI GKGCMC 2 cut(s) 1055, 1719
BseXI GCAGC 7 cut(s) 953, 1183, 1430, 1883, 2295, 2401, 2611
BseYI CCCAGC 3 cut(s) 221, 623, 1930
BshFI GGCC 7 cut(s) 30, 536, 1335, 1521, 1837, 2177, 2706
BshNI GGYRCC 1 cut(s) 984
BshVI ATCGAT 1 cut(s) 2421
BsiHKAI GWGCWC 2 cut(s) 1292, 1912
BsiHKCI CYCGRG 5 cut(s) 301, 400, 599, 1291, 2021
BsiSI CCGG 6 cut(s) 120, 946, 988, 1239, 1701, 1993
BslFI GGGAC 3 cut(s) 502, 2003, 2667
BslI CCNNNNNNNGG 7 cut(s) 301, 1224, 1245, 1778, 1887, 1987, 2714
BsmAI GTCTC 8 cut(s) 323, 430, 675, 864, 1711, 1818, 1990, 2515
BsmBI CGTCTC 1 cut(s) 675
BsmFI GGGAC 3 cut(s) 502, 2003, 2667
BsmI GAATGC 3 cut(s) 955, 2070, 2498
BsnI GGCC 7 cut(s) 30, 536, 1335, 1521, 1837, 2177, 2706
BsoBI CYCGRG 5 cut(s) 301, 400, 599, 1291, 2021
Bsp119I TTCGAA 1 cut(s) 734
Bsp1286I GDGCHC 5 cut(s) 600, 1055, 1292, 1719, 1912
Bsp1407I TGTACA 1 cut(s) 1408
Bsp143I GATC 7 cut(s) 178, 1137, 1452, 1748, 2242, 2515, 2719
BspACI CCGC 4 cut(s) 347, 1550, 1888, 2332
BspANI GGCC 7 cut(s) 30, 536, 1335, 1521, 1837, 2177, 2706
BspCNI CTCAG 5 cut(s) 406, 832, 1024, 1519, 1828
BspDI ATCGAT 1 cut(s) 2421
BspLI GGNNCC 5 cut(s) 986, 1821, 1991, 2223, 2707
BspMAI CTGCAG 2 cut(s) 1201, 1420
BspMI ACCTGC 2 cut(s) 67, 2705
BspPI GGATC 5 cut(s) 186, 1132, 1460, 1756, 2510
BspT104I TTCGAA 1 cut(s) 734
BspT107I GGYRCC 1 cut(s) 984
BspTI CTTAAG 1 cut(s) 259
BsrDI GCAATG 3 cut(s) 752, 1941, 2491
BsrFI RCCGGY 1 cut(s) 119
BsrGI TGTACA 1 cut(s) 1408
BsrI ACTGG 1 cut(s) 1381
BssAI RCCGGY 1 cut(s) 119
BssECI CCNNGG 3 cut(s) 904, 1567, 1970
BssMI GATC 7 cut(s) 178, 1137, 1452, 1748, 2242, 2515, 2719
BssNAI GTATAC 1 cut(s) 2394
BssNI GRCGYC 1 cut(s) 985
Bst1107I GTATAC 1 cut(s) 2394
Bst2UI CCWGG 8 cut(s) 723, 1272, 1779, 1824, 1971, 2043, 2174, 2205
Bst4CI ACNGT 5 cut(s) 166, 905, 1302, 1408, 2313
Bst6I CTCTTC 3 cut(s) 495, 1238, 2696
BstACI GRCGYC 1 cut(s) 985
BstAFI CTTAAG 1 cut(s) 259
BstAPI GCANNNNNTGC 3 cut(s) 1205, 1635, 2597
BstAUI TGTACA 1 cut(s) 1408
BstBAI YACGTR 1 cut(s) 1724
BstBI TTCGAA 1 cut(s) 734
BstC8I GCNNGC 3 cut(s) 895, 1546, 1550
BstDEI CTNAG 5 cut(s) 414, 840, 1032, 1527, 1815
BstDSI CCRYGG 2 cut(s) 904, 1567
BstF5I GGATG 9 cut(s) 634, 675, 1330, 1563, 1585, 1876, 1896, 2038, 2353
BstH2I RGCGCY 2 cut(s) 988, 2431
BstHHI GCGC 2 cut(s) 987, 2430
BstKTI GATC 7 cut(s) 181, 1140, 1455, 1751, 2245, 2518, 2722
BstMAI GTCTC 8 cut(s) 323, 430, 675, 864, 1711, 1818, 1990, 2515
BstMBI GATC 7 cut(s) 178, 1137, 1452, 1748, 2242, 2515, 2719
BstNI CCWGG 8 cut(s) 723, 1272, 1779, 1824, 1971, 2043, 2174, 2205
BstNSI RCATGY 5 cut(s) 106, 119, 1441, 1548, 2113
BstPAI GACNNNNGTC 1 cut(s) 442
BstSFI CTRYAG 3 cut(s) 1197, 1298, 1416
BstSLI GKGCMC 2 cut(s) 1055, 1719
BstV1I GCAGC 7 cut(s) 953, 1183, 1430, 1883, 2295, 2401, 2611
BstV2I GAAGAC 1 cut(s) 840
BstX2I RGATCY 3 cut(s) 1137, 1452, 1748
BstYI RGATCY 3 cut(s) 1137, 1452, 1748
BstZ17I GTATAC 1 cut(s) 2394
Bsu15I ATCGAT 1 cut(s) 2421
BsuI GTATCC 1 cut(s) 2211
BsuRI GGCC 7 cut(s) 30, 536, 1335, 1521, 1837, 2177, 2706
BsuTUI ATCGAT 1 cut(s) 2421
BtgI CCRYGG 2 cut(s) 904, 1567
BtgZI GCGATG 2 cut(s) 968, 2689
BtsCI GGATG 9 cut(s) 634, 675, 1330, 1563, 1585, 1876, 1896, 2038, 2353
BtsI GCAGTG 3 cut(s) 618, 757, 1657
BtsIMutI CAGTG 3 cut(s) 618, 757, 1657
BveI ACCTGC 2 cut(s) 67, 2705
Cac8I GCNNGC 3 cut(s) 895, 1546, 1550
CaiI CAGNNNCTG 2 cut(s) 764, 1205
CfoI GCGC 2 cut(s) 987, 2430
Cfr10I RCCGGY 1 cut(s) 119
Cfr13I GGNCC 5 cut(s) 28, 535, 1990, 2176, 2705
ClaI ATCGAT 1 cut(s) 2421
Csp6I GTAC 2 cut(s) 1409, 1466
CspCI CAANNNNNGTGG 2 cut(s) 886, 921
CviQI GTAC 2 cut(s) 1409, 1466
DdeI CTNAG 5 cut(s) 414, 840, 1032, 1527, 1815
DinI GGCGCC 1 cut(s) 986
DpnI GATC 7 cut(s) 180, 1139, 1454, 1750, 2244, 2517, 2721
DpnII GATC 7 cut(s) 178, 1137, 1452, 1748, 2242, 2515, 2719
EaeI YGGCCR 2 cut(s) 1519, 1835
Eam1104I CTCTTC 3 cut(s) 495, 1238, 2696
EarI CTCTTC 3 cut(s) 495, 1238, 2696
Ecl136II GAGCTC 1 cut(s) 1290
Eco24I GRGCYC 2 cut(s) 600, 1292
Eco32I GATATC 1 cut(s) 921
Eco47I GGWCC 1 cut(s) 1990
Eco53kI GAGCTC 1 cut(s) 1290
Eco57I CTGAAG 1 cut(s) 30
Eco72I CACGTG 1 cut(s) 1724
Eco88I CYCGRG 5 cut(s) 301, 400, 599, 1291, 2021
EcoICRI GAGCTC 1 cut(s) 1290
EcoO109I RGGNCCY 1 cut(s) 28
EcoRI GAATTC 2 cut(s) 2233, 2610
EcoRII CCWGG 8 cut(s) 721, 1270, 1777, 1822, 1969, 2041, 2172, 2203
EcoRV GATATC 1 cut(s) 921
EcoT22I ATGCAT 3 cut(s) 1795, 1896, 2500
EcoT38I GRGCYC 2 cut(s) 600, 1292
EgeI GGCGCC 1 cut(s) 986
EheI GGCGCC 1 cut(s) 986
Esp3I CGTCTC 1 cut(s) 675
FaqI GGGAC 3 cut(s) 502, 2003, 2667
FauI CCCGC 1 cut(s) 1557
FauNDI CATATG 3 cut(s) 1920, 2005, 2443
FbaI TGATCA 2 cut(s) 2242, 2719
FblI GTMKAC 1 cut(s) 2393
Fnu4HI GCNGC 7 cut(s) 942, 1197, 1419, 1872, 2284, 2415, 2625
FokI GGATG 9 cut(s) 641, 682, 1337, 1550, 1572, 1863, 1903, 2025, 2360
FriOI GRGCYC 2 cut(s) 600, 1292
Fsp4HI GCNGC 7 cut(s) 942, 1197, 1419, 1872, 2284, 2415, 2625
FspBI CTAG 4 cut(s) 795, 1362, 1461, 1905
GlaI GCGC 2 cut(s) 986, 2429
GluI GCNGC 7 cut(s) 942, 1197, 1419, 1872, 2284, 2415, 2625
GsaI CCCAGC 3 cut(s) 225, 627, 1934
GsuI CTGGAG 7 cut(s) 603, 744, 786, 950, 1293, 1806, 2156
HaeII RGCGCY 2 cut(s) 988, 2431
HaeIII GGCC 7 cut(s) 30, 536, 1335, 1521, 1837, 2177, 2706
HapII CCGG 6 cut(s) 120, 946, 988, 1239, 1701, 1993
HhaI GCGC 2 cut(s) 987, 2430
Hin1I GRCGYC 1 cut(s) 985
Hin6I GCGC 2 cut(s) 985, 2428
HinP1I GCGC 2 cut(s) 985, 2428
HincII GTYRAC 1 cut(s) 2299
HindII GTYRAC 1 cut(s) 2299
HindIII AAGCTT 2 cut(s) 992, 1253
HpaII CCGG 6 cut(s) 120, 946, 988, 1239, 1701, 1993
HphI GGTGA 9 cut(s) 134, 173, 178, 226, 794, 1566, 1836, 2070, 2729
Hpy166II GTNNAC 7 cut(s) 785, 1411, 1466, 1727, 1765, 2299, 2394
Hpy8I GTNNAC 7 cut(s) 785, 1411, 1466, 1727, 1765, 2299, 2394
HpyAV CCTTC 6 cut(s) 344, 563, 801, 1038, 2273, 2578
HpyCH4III ACNGT 5 cut(s) 166, 905, 1302, 1408, 2313
HpyCH4IV ACGT 3 cut(s) 1723, 1761, 2257
HpyF3I CTNAG 5 cut(s) 414, 840, 1032, 1527, 1815
HpySE526I ACGT 3 cut(s) 1723, 1761, 2257
Hsp92I GRCGYC 1 cut(s) 985
HspAI GCGC 2 cut(s) 985, 2428
KasI GGCGCC 1 cut(s) 984
Ksp22I TGATCA 2 cut(s) 2242, 2719
Kzo9I GATC 7 cut(s) 178, 1137, 1452, 1748, 2242, 2515, 2719
LmnI GCTCC 5 cut(s) 1287, 1394, 1426, 1825, 2536
Lsp1109I GCAGC 7 cut(s) 953, 1183, 1430, 1883, 2295, 2401, 2611
LweI GCATC 7 cut(s) 146, 344, 1638, 1881, 2394, 2600, 2666
MaeI CTAG 4 cut(s) 795, 1362, 1461, 1905
MaeII ACGT 3 cut(s) 1723, 1761, 2257
MaeIII GTNAC 6 cut(s) 129, 586, 824, 1302, 1364, 1757
MalI GATC 7 cut(s) 180, 1139, 1454, 1750, 2244, 2517, 2721
MboI GATC 7 cut(s) 178, 1137, 1452, 1748, 2242, 2515, 2719
MfeI CAATTG 2 cut(s) 2463, 2595
MflI RGATCY 3 cut(s) 1137, 1452, 1748
MhlI GDGCHC 5 cut(s) 600, 1055, 1292, 1719, 1912
MlsI TGGCCA 2 cut(s) 1521, 1837
MluNI TGGCCA 2 cut(s) 1521, 1837
Mly113I GGCGCC 1 cut(s) 985
MlyI GAGTC 8 cut(s) 293, 524, 830, 874, 1538, 1713, 2033, 2163
MmeI TCCRAC 2 cut(s) 624, 2070
Mox20I TGGCCA 2 cut(s) 1521, 1837
Mph1103I ATGCAT 3 cut(s) 1795, 1896, 2500
MroXI GAANNNNTTC 1 cut(s) 1439
MscI TGGCCA 2 cut(s) 1521, 1837
MslI CAYNNNNRTG 8 cut(s) 120, 777, 1058, 1722, 1788, 2124, 2160, 2507
Msp20I TGGCCA 2 cut(s) 1521, 1837
MspA1I CMGCKG 3 cut(s) 698, 1552, 1888
MspCI CTTAAG 1 cut(s) 259
MspI CCGG 6 cut(s) 120, 946, 988, 1239, 1701, 1993
MunI CAATTG 2 cut(s) 2463, 2595
Mva1269I GAATGC 3 cut(s) 955, 2070, 2498
MvaI CCWGG 8 cut(s) 723, 1272, 1779, 1824, 1971, 2043, 2174, 2205
NarI GGCGCC 1 cut(s) 985
NciI CCSGG 2 cut(s) 946, 1240
NdeI CATATG 3 cut(s) 1920, 2005, 2443
NdeII GATC 7 cut(s) 178, 1137, 1452, 1748, 2242, 2515, 2719
NlaIV GGNNCC 5 cut(s) 986, 1821, 1991, 2223, 2707
NmuCI GTSAC 1 cut(s) 129
NsiI ATGCAT 3 cut(s) 1795, 1896, 2500
NspI RCATGY 5 cut(s) 106, 119, 1441, 1548, 2113
NspV TTCGAA 1 cut(s) 734
OliI CACNNNNGTG 2 cut(s) 1058, 1722
PaeI GCATGC 1 cut(s) 1548
PaeR7I CTCGAG 2 cut(s) 400, 599
PaqCI CACCTGC 1 cut(s) 2705
PciI ACATGT 2 cut(s) 1437, 2109
PctI GAATGC 3 cut(s) 955, 2070, 2498
PdmI GAANNNNTTC 1 cut(s) 1439
PfeI GAWTC 5 cut(s) 1012, 1076, 1202, 1499, 2015
PfoI TCCNGGA 5 cut(s) 721, 1270, 1777, 1822, 2203
PkrI GCNGC 7 cut(s) 943, 1198, 1420, 1873, 2285, 2416, 2626
PleI GAGTC 8 cut(s) 293, 523, 830, 874, 1537, 1712, 2032, 2163
PluTI GGCGCC 1 cut(s) 988
PmaCI CACGTG 1 cut(s) 1724
PmlI CACGTG 1 cut(s) 1724
PpsI GAGTC 8 cut(s) 293, 523, 830, 874, 1537, 1712, 2032, 2163
Ppu21I YACGTR 1 cut(s) 1724
PscI ACATGT 2 cut(s) 1437, 2109
PshAI GACNNNNGTC 1 cut(s) 442
Psp124BI GAGCTC 1 cut(s) 1292
Psp6I CCWGG 8 cut(s) 721, 1270, 1777, 1822, 1969, 2041, 2172, 2203
PspCI CACGTG 1 cut(s) 1724
PspFI CCCAGC 3 cut(s) 221, 623, 1930
PspGI CCWGG 8 cut(s) 721, 1270, 1777, 1822, 1969, 2041, 2172, 2203
PspN4I GGNNCC 5 cut(s) 986, 1821, 1991, 2223, 2707
PspPI GGNCC 5 cut(s) 28, 535, 1990, 2176, 2705
PspXI VCTCGAGB 1 cut(s) 599
PstI CTGCAG 2 cut(s) 1201, 1420
PstNI CAGNNNCTG 2 cut(s) 764, 1205
PsuI RGATCY 3 cut(s) 1137, 1452, 1748
PvuII CAGCTG 1 cut(s) 698
RsaI GTAC 2 cut(s) 1410, 1467
RsaNI GTAC 2 cut(s) 1409, 1466
RseI CAYNNNNRTG 8 cut(s) 120, 777, 1058, 1722, 1788, 2124, 2160, 2507
SacI GAGCTC 1 cut(s) 1292
SatI GCNGC 7 cut(s) 942, 1197, 1419, 1872, 2284, 2415, 2625
Sau3AI GATC 7 cut(s) 178, 1137, 1452, 1748, 2242, 2515, 2719
Sau96I GGNCC 5 cut(s) 28, 535, 1990, 2176, 2705
SchI GAGTC 8 cut(s) 293, 524, 830, 874, 1538, 1713, 2033, 2163
SduI GDGCHC 5 cut(s) 600, 1055, 1292, 1719, 1912
SfaNI GCATC 7 cut(s) 146, 344, 1638, 1881, 2394, 2600, 2666
SfcI CTRYAG 3 cut(s) 1197, 1298, 1416
SfoI GGCGCC 1 cut(s) 986
Sfr274I CTCGAG 2 cut(s) 400, 599
SfuI TTCGAA 1 cut(s) 734
SinI GGWCC 1 cut(s) 1990
SlaI CTCGAG 2 cut(s) 400, 599
SmiMI CAYNNNNRTG 8 cut(s) 120, 777, 1058, 1722, 1788, 2124, 2160, 2507
SmlI CTYRAG 8 cut(s) 149, 259, 400, 599, 1256, 1308, 1708, 2650
SmoI CTYRAG 8 cut(s) 149, 259, 400, 599, 1256, 1308, 1708, 2650
SphI GCATGC 1 cut(s) 1548
SsiI CCGC 4 cut(s) 347, 1550, 1888, 2332
SspDI GGCGCC 1 cut(s) 984
SspI AATATT 1 cut(s) 2380
SspMI CTAG 4 cut(s) 795, 1362, 1461, 1905
SstI GAGCTC 1 cut(s) 1292
TaaI ACNGT 5 cut(s) 166, 905, 1302, 1408, 2313
TaiI ACGT 3 cut(s) 1726, 1764, 2260
TaqI TCGA 8 cut(s) 281, 401, 600, 734, 1010, 1176, 2421, 2608
TatI WGTACW 1 cut(s) 1408
TfiI GAWTC 5 cut(s) 1012, 1076, 1202, 1499, 2015
TscAI CASTG 3 cut(s) 625, 757, 1657
TseFI GTSAC 1 cut(s) 129
TseI GCWGC 7 cut(s) 941, 1196, 1418, 1871, 2283, 2414, 2624
Tsp45I GTSAC 1 cut(s) 129
TspGWI ACGGA 2 cut(s) 882, 1130
TspRI CASTG 3 cut(s) 625, 757, 1657
Vha464I CTTAAG 1 cut(s) 259
VpaK11BI GGWCC 1 cut(s) 1990
XapI RAATTY 8 cut(s) 274, 507, 1178, 1556, 1863, 2093, 2233, 2610
XceI RCATGY 5 cut(s) 106, 119, 1441, 1548, 2113
XcmI CCANNNNNNNNNTGG 1 cut(s) 1830
XhoI CTCGAG 2 cut(s) 400, 599
XmiI GTMKAC 1 cut(s) 2393
XmnI GAANNNNTTC 1 cut(s) 1439
XspI CTAG 4 cut(s) 795, 1362, 1461, 1905
Zsp2I ATGCAT 3 cut(s) 1795, 1896, 2500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.