Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0012427

Physical Location & Seq

Genomic Coordinates Forward (+)

12563071 .. 12566401

Gene Structure 3331 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1159600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1974 bp

ATGGACTCGAGCTCCAGGAACTGGCCTCCGATCTTGGCCCTCAATCTTCTGCTTATTTTCATGATCCTCCCCTTCTCACTCGCCCAATCCACCGCCCAAGCTCCGCCGCCGGCCTCCGACTCCTGCAACGGCGTCTTCCTGTCGTACGCCTACACCACCGGAGCCCAGCTCCCGCCCGAGGTCAAAGACCCGTCCCAGCAGCCCTACCGGTTCGAGTCGGTGCTCACGGTGCTCAACAACGGGCTCGACGATCTCAAGTCGTGGAGGGTGTTTGTGGGGTTTACTAACAATGAGTACTTGGTCTCTGCCTCCAATGCCGTTTTGGCCGACGGGAGTAGCATTCCTGGGAGTGTGGGAAACGGCACCGTTTTTGCTGGGTATCCGATGACCGATCTGAAAACGGCGGTTAAGACGGCGGGGGACTTGACCCAGATGCAGGTCCAGGTCAAATTGATCGGGACCCAGTTCGGTGTGGCCCCACCCAAAGTTCCCATGCCTTCCAATCTTACTTTGGCCAATGATGGATTCGTCTGCCCAGCGGCTGCAATGCAAGGGACCCATGAAATGCAAGTCTGTTGCACCGTAGACACAAAATTTAAAACAAACATCACTGTGGATGAAGAGTTCCTCCCCCGTCAAAAAGGAGATCTTACAATTATGTATGATGTGATCAGTACTCAGGAGTCAAAATACACGGCGCAAGTTACAATCGCCAACCATAACTCCCTTGGTCGTCTTGATAATTGGAAATTGAGCTGGGACTGGATGGAAGATGAGTTCATATTTTCAATGAAGGGGGCTTATCCATCTGTTGTTGATTCTTCTGACTGTATATTTGGTAAACAAGGTACATTCTATGAGGGTCTAGACTTCTCAACTGTGTTGAACTGTGAAAGACGGCCAACCATTATTGACCTGCCTCCAACAAAAGCCAATGACACACTTCTTGGTCTGGTCCCTTATTGTTGCCGAAATGGTACTATCTTGCCGCCAACAATGGATGCAAGCAAGTCAGTTTCATCATTCCAGATTCAAGTTTTTAAAATGCCTCCAAATCTCAACCGGTCCCAGCTCTCACCGCCACAAAACTGGGGAATCAACGGCACGCTCAACCCTGATTATAAATGTGGCCCTCCTGTCCAGGTGAGTCCTAGCCAGTTCCCTGACCGATCTGGCTTGCCAGTGAATTCAACTGCAGTAGCCAGCTGGCAGGTTGTGTGCAATATTACCCAACCCAAGGGAGCAAGCCCTAGATGCTGTGTTTCGTTTTCTGCTTTCTACAATGAGTCTGTCATCCCATGCAACACTTGCGCTTGTGGCTGCCCTAGTAATATAGCTCGAACTTGTAGCACAACCGCACCAGCTATGCTTCTCCCACCAGAATCACTTCTTGTTCCCTTTGACAACCGAACTGTCAAGGCAAAAGCTTGGGCTGAGCTTAAACATCTACCAGACCCAAACCCGACACCGTGTGGTGATAACTGTGGGGTCAGCATTAACTGGCATTTATATACAGACTACTCTCGTGGATGGAGTGCAAGGGTCACAGTCTTCAATTGGGATGAAACTGCTTTTGTTGATTGGTTTGCTGCAGTACAACTGGATAAAGCAGGCCCTGGTTTTGAAAAGGCGTACTCTTTCAACGCAAGTCACTTGGAATTGAATGGTGTCAATAATACCGTATTCATGCAAGGTCTCGAAGGATTGAACTATCTTGTGGCGGAAACAGATGGAGCCAACCCACAGAAGGATCCTAGGGTGCCCGGGAAACAGCAATCAGTGATCTCATTTACAAAGAAGACTACTCCTGGAATCAATTTGGTTGGTGGAGATGGGTTTCCGACGAAAGTATACTTTAACGGGGAGGAGTGCTCACTTCCTACAATATATCCTAGCAGTGCTTACAGAAAAAGCACATCTATAATTTTCTCACTTCTCGTAATGGTTGTAGCGTTCGTGGTGCTGCAGCAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005886 GO:0005911 GO:0007275 GO:0008150 GO:0009506 GO:0009653 GO:0009826 GO:0009888 GO:0009932 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0021700 GO:0022622 GO:0030054 GO:0030154 GO:0031224 GO:0031225 GO:0031226 GO:0031410 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0032989 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046658 GO:0048364 GO:0048468 GO:0048469 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048856 GO:0048869 GO:0055044 GO:0060560 GO:0071695 GO:0071840 GO:0071944 GO:0090558 GO:0090627 GO:0097708 GO:0098791 GO:0099402 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

COBRA

Protein Analysis

658

Amino Acids

71.55

Weight (kDa)

5.26

Isoelectric Point (pI)

36.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
COBRA	PF04833	231 - 410	8.6e-65	COBRA-like protein
COB_C	PF25079	418 - 631	3.8e-100	COBRA family C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012427)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G16120
fragaria_vesca	FvH4_4g19240
malus_domestica	MD13G1159600.v1.1 MD16G1159300.v1.1
prunus_persica	Prupe.1G126300_v2.0.a1
pyrus_communis	pycom13g13600 pycom16g13430
rosa_chinensis	RchiOBHm_Chr4g0424221
rosa_laevigata	RLG00000007495
rosa_multiflora	Rmu_sc0000980.1_g000001 Rmu_sc0002260.1_g000034 Rmu_sc0019001.1_g000001
rosa_roxburghii	Rroxscaffold_5G00366170
rosa_rugosa	Rorug04G0194400
rosa_samantha	Rh4AG251800 Rh4BG257100 Rh4CG267700 Rh4DG251500
rosa_wichuraiana	Rw4G021710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1122
Acc36I	ACCTGC	3 cut(s)	427, 924, 1201
AccB1I	GGYRCC	2 cut(s)	362, 1759
AccB7I	CCANNNNNTGG	1 cut(s)	21
AccI	GTMKAC	2 cut(s)	585, 1851
AclWI	GGATC	3 cut(s)	58, 1745, 1758
AcoI	YGGCCR	3 cut(s)	324, 513, 899
AcsI	RAATTY	2 cut(s)	593, 1186
AcyI	GRCGYC	1 cut(s)	132
AdeI	CACNNNGTG	1 cut(s)	1472
AfaI	GTAC	7 cut(s)	146, 296, 676, 850, 979, 1596, 1634
AfiI	CCNNNNNNNGG	5 cut(s)	21, 178, 436, 1237, 1747
AgeI	ACCGGT	2 cut(s)	207, 1062
AgsI	TTSAA	9 cut(s)	789, 886, 1034, 1191, 1555, 1625, 1642, 1663, 1708
AjnI	CCWGG	6 cut(s)	14, 343, 441, 1140, 1615, 1807
AjuI	GAANNNNNNNTTGG	2 cut(s)	509, 541
AloI	GAACNNNNNNTCC	4 cut(s)	608, 640, 761, 793
Alw21I	GWGCWC	4 cut(s)	14, 225, 234, 1874
Alw26I	GTCTC	2 cut(s)	307, 1700
AlwI	GGATC	3 cut(s)	58, 1745, 1758
AlwNI	CAGNNNCTG	3 cut(s)	21, 542, 1616
Ama87I	CYCGRG	3 cut(s)	7, 176, 1763
AoxI	GGCC	9 cut(s)	23, 36, 111, 324, 474, 513, 899, 1129, 1612
ApeKI	GCWGC	6 cut(s)	199, 542, 1320, 1589, 1963, 1966
ApoI	RAATTY	2 cut(s)	593, 1186
ArsI	GACNNNNNNTTYG	2 cut(s)	818, 850
AsiGI	ACCGGT	2 cut(s)	207, 1062
Asp700I	GAANNNNTTC	1 cut(s)	1386
AspA2I	CCTAGG	1 cut(s)	1754
AspLEI	GCGC	2 cut(s)	700, 1313
AspS9I	GGNCC	9 cut(s)	37, 439, 459, 475, 555, 955, 1065, 1130, 1613
AsuC2I	CCSGG	2 cut(s)	1764, 1765
AsuHPI	GGTGA	3 cut(s)	1068, 1156, 1487
AvaI	CYCGRG	3 cut(s)	7, 176, 1763
AvaII	GGWCC	5 cut(s)	439, 459, 555, 955, 1065
AvrII	CCTAGG	1 cut(s)	1754
BaeGI	GKGCMC	1 cut(s)	1764
BalI	TGGCCA	1 cut(s)	515
BamHI	GGATCC	1 cut(s)	1750
BanI	GGYRCC	2 cut(s)	362, 1759
BanII	GRGCYC	3 cut(s)	14, 166, 246
BauI	CACGAG	1 cut(s)	1524
BbsI	GAAGAC	3 cut(s)	127, 1543, 1805
Bbv12I	GWGCWC	4 cut(s)	14, 225, 234, 1874
BbvI	GCAGC	5 cut(s)	211, 529, 1307, 1576, 1950
BccI	CCATC	6 cut(s)	515, 760, 814, 1524, 1724, 1826
BceAI	ACGGC	8 cut(s)	145, 302, 376, 417, 429, 711, 914, 1117
BciT130I	CCWGG	6 cut(s)	16, 345, 443, 1142, 1617, 1809
BciVI	GTATCC	1 cut(s)	390
BclI	TGATCA	1 cut(s)	669
BcnI	CCSGG	2 cut(s)	1764, 1765
BcoDI	GTCTC	2 cut(s)	307, 1700
BfaI	CTAG	6 cut(s)	866, 1152, 1251, 1326, 1755, 1893
BfmI	CTRYAG	3 cut(s)	1194, 1590, 1964
BfuAI	ACCTGC	3 cut(s)	427, 924, 1201
BfuI	GTATCC	1 cut(s)	390
BglI	GCCNNNNNGGC	1 cut(s)	323
BglII	AGATCT	1 cut(s)	646
BisI	GCNGC	9 cut(s)	107, 200, 540, 543, 989, 1321, 1590, 1964, 1967
BlnI	CCTAGG	1 cut(s)	1754
BlpI	GCTNAGC	1 cut(s)	1434
BlsI	GCNGC	9 cut(s)	108, 201, 541, 544, 990, 1322, 1591, 1965, 1968
BmcAI	AGTACT	2 cut(s)	296, 676
Bme1390I	CCNGG	8 cut(s)	16, 345, 443, 1142, 1617, 1764, 1765, 1809
Bme18I	GGWCC	5 cut(s)	439, 459, 555, 955, 1065
BmeT110I	CYCGRG	3 cut(s)	7, 176, 1763
BmgT120I	GGNCC	9 cut(s)	37, 439, 459, 475, 555, 955, 1065, 1130, 1613
BmrFI	CCNGG	8 cut(s)	16, 345, 443, 1142, 1617, 1764, 1765, 1809
BmrI	ACTGGG	2 cut(s)	457, 1099
BmsI	GCATC	3 cut(s)	423, 991, 1244
BmuI	ACTGGG	2 cut(s)	457, 1099
BpiI	GAAGAC	3 cut(s)	127, 1543, 1805
BplI	GAGNNNNNCTC	4 cut(s)	153, 185, 1856, 1888
Bpu1102I	GCTNAGC	1 cut(s)	1434
BpuEI	CTTGAG	1 cut(s)	239
BpuMI	CCSGG	2 cut(s)	1764, 1765
BsaHI	GRCGYC	1 cut(s)	132
BsaI	GGTCTC	2 cut(s)	307, 1700
BsaJI	CCNNGG	7 cut(s)	177, 344, 727, 1236, 1615, 1754, 1763
BsaWI	WCCGGW	3 cut(s)	158, 207, 1062
BsaXI	ACNNNNNCTCC	3 cut(s)	26, 707, 737
Bsc4I	CCNNNNNNNGG	5 cut(s)	21, 178, 436, 1237, 1747
Bse118I	RCCGGY	3 cut(s)	109, 207, 1062
Bse1I	ACTGG	8 cut(s)	26, 463, 767, 1094, 1156, 1181, 1505, 1605
Bse3DI	GCAATG	1 cut(s)	552
BseBI	CCWGG	6 cut(s)	16, 345, 443, 1142, 1617, 1809
BseDI	CCNNGG	7 cut(s)	177, 344, 727, 1236, 1615, 1754, 1763
BseGI	GGATG	6 cut(s)	622, 771, 1006, 1293, 1535, 1567
BseLI	CCNNNNNNNGG	5 cut(s)	21, 178, 436, 1237, 1747
BseMI	GCAATG	1 cut(s)	552
BseMII	CTCAG	2 cut(s)	692, 1425
BseNI	ACTGG	8 cut(s)	26, 463, 767, 1094, 1156, 1181, 1505, 1605
BseRI	GAGGAG	1 cut(s)	1880
BseSI	GKGCMC	1 cut(s)	1764
BseXI	GCAGC	5 cut(s)	211, 529, 1307, 1576, 1950
BseYI	CCCAGC	6 cut(s)	165, 195, 374, 535, 756, 1068
BshFI	GGCC	9 cut(s)	25, 38, 113, 326, 476, 515, 901, 1131, 1614
BshNI	GGYRCC	2 cut(s)	362, 1759
BshTI	ACCGGT	2 cut(s)	207, 1062
BsiHKAI	GWGCWC	4 cut(s)	14, 225, 234, 1874
BsiHKCI	CYCGRG	3 cut(s)	7, 176, 1763
BsiSI	CCGG	5 cut(s)	110, 159, 208, 1063, 1764
BsiWI	CGTACG	1 cut(s)	144
BslFI	GGGAC	7 cut(s)	178, 434, 472, 568, 773, 941, 1051
BslI	CCNNNNNNNGG	5 cut(s)	21, 178, 436, 1237, 1747
BsmAI	GTCTC	2 cut(s)	307, 1700
BsmFI	GGGAC	7 cut(s)	178, 434, 472, 568, 773, 941, 1051
BsmI	GAATGC	1 cut(s)	339
BsnI	GGCC	9 cut(s)	25, 38, 113, 326, 476, 515, 901, 1131, 1614
Bso31I	GGTCTC	2 cut(s)	307, 1700
BsoBI	CYCGRG	3 cut(s)	7, 176, 1763
Bsp1286I	GDGCHC	7 cut(s)	14, 166, 225, 234, 246, 1764, 1874
Bsp1720I	GCTNAGC	1 cut(s)	1434
BspANI	GGCC	9 cut(s)	25, 38, 113, 326, 476, 515, 901, 1131, 1614
BspCNI	CTCAG	2 cut(s)	691, 1426
BspHI	TCATGA	1 cut(s)	60
BspMAI	CTGCAG	3 cut(s)	1198, 1594, 1968
BspMI	ACCTGC	3 cut(s)	427, 924, 1201
BspPI	GGATC	3 cut(s)	58, 1745, 1758
BspT107I	GGYRCC	2 cut(s)	362, 1759
BspTNI	GGTCTC	2 cut(s)	307, 1700
BsrDI	GCAATG	1 cut(s)	552
BsrFI	RCCGGY	3 cut(s)	109, 207, 1062
BsrI	ACTGG	8 cut(s)	26, 463, 767, 1094, 1156, 1181, 1505, 1605
BssAI	RCCGGY	3 cut(s)	109, 207, 1062
BssECI	CCNNGG	7 cut(s)	177, 344, 727, 1236, 1615, 1754, 1763
BssNAI	GTATAC	1 cut(s)	1852
BssNI	GRCGYC	1 cut(s)	132
BssSI	CACGAG	1 cut(s)	1524
BssT1I	CCWWGG	3 cut(s)	727, 1236, 1754
Bst1107I	GTATAC	1 cut(s)	1852
Bst2BI	CACGAG	1 cut(s)	1524
Bst2UI	CCWGG	6 cut(s)	16, 345, 443, 1142, 1617, 1809
Bst6I	CTCTTC	1 cut(s)	615
BstACI	GRCGYC	1 cut(s)	132
BstAPI	GCANNNNNTGC	1 cut(s)	1308
BstC8I	GCNNGC	8 cut(s)	111, 1006, 1106, 1178, 1204, 1208, 1246, 1612
BstDEI	CTNAG	2 cut(s)	678, 1434
BstF5I	GGATG	6 cut(s)	622, 771, 1006, 1293, 1535, 1567
BstHHI	GCGC	2 cut(s)	700, 1313
BstMAI	GTCTC	2 cut(s)	307, 1700
BstMWI	GCNNNNNNNGC	7 cut(s)	229, 314, 323, 1078, 1254, 1308, 1317
BstNI	CCWGG	6 cut(s)	16, 345, 443, 1142, 1617, 1809
BstSCI	CCNGG	8 cut(s)	14, 343, 441, 1140, 1615, 1762, 1763, 1807
BstSFI	CTRYAG	3 cut(s)	1194, 1590, 1964
BstSLI	GKGCMC	1 cut(s)	1764
BstV1I	GCAGC	5 cut(s)	211, 529, 1307, 1576, 1950
BstV2I	GAAGAC	3 cut(s)	127, 1543, 1805
BstX2I	RGATCY	2 cut(s)	646, 1750
BstXI	CCANNNNNNTGG	1 cut(s)	1089
BstYI	RGATCY	2 cut(s)	646, 1750
BstZ17I	GTATAC	1 cut(s)	1852
BsuI	GTATCC	1 cut(s)	390
BsuRI	GGCC	9 cut(s)	25, 38, 113, 326, 476, 515, 901, 1131, 1614
BtsCI	GGATG	6 cut(s)	622, 771, 1006, 1293, 1535, 1567
BtsI	GCAGTG	1 cut(s)	1903
BtsIMutI	CAGTG	4 cut(s)	609, 1188, 1785, 1903
BveI	ACCTGC	3 cut(s)	427, 924, 1201
Cac8I	GCNNGC	8 cut(s)	111, 1006, 1106, 1178, 1204, 1208, 1246, 1612
CaiI	CAGNNNCTG	3 cut(s)	21, 542, 1616
CciI	TCATGA	1 cut(s)	60
CfoI	GCGC	2 cut(s)	700, 1313
Cfr10I	RCCGGY	3 cut(s)	109, 207, 1062
Cfr13I	GGNCC	9 cut(s)	37, 439, 459, 475, 555, 955, 1065, 1130, 1613
Cfr9I	CCCGGG	1 cut(s)	1763
CseI	GACGC	1 cut(s)	121
Csp6I	GTAC	7 cut(s)	145, 295, 675, 849, 978, 1595, 1633
CspAI	ACCGGT	2 cut(s)	207, 1062
CviAII	CATG	5 cut(s)	61, 493, 560, 1299, 1687
CviQI	GTAC	7 cut(s)	145, 295, 675, 849, 978, 1595, 1633
DdeI	CTNAG	2 cut(s)	678, 1434
DraI	TTTAAA	2 cut(s)	598, 1042
DraIII	CACNNNGTG	1 cut(s)	1472
EaeI	YGGCCR	3 cut(s)	324, 513, 899
Eam1104I	CTCTTC	1 cut(s)	615
EarI	CTCTTC	1 cut(s)	615
EciI	GGCGGA	2 cut(s)	93, 1736
Ecl136II	GAGCTC	1 cut(s)	12
Eco130I	CCWWGG	3 cut(s)	727, 1236, 1754
Eco24I	GRGCYC	3 cut(s)	14, 166, 246
Eco31I	GGTCTC	2 cut(s)	307, 1700
Eco47I	GGWCC	5 cut(s)	439, 459, 555, 955, 1065
Eco53kI	GAGCTC	1 cut(s)	12
Eco88I	CYCGRG	3 cut(s)	7, 176, 1763
EcoICRI	GAGCTC	1 cut(s)	12
EcoO109I	RGGNCCY	3 cut(s)	459, 555, 1613
EcoRI	GAATTC	1 cut(s)	1186
EcoRII	CCWGG	6 cut(s)	14, 343, 441, 1140, 1615, 1807
EcoT14I	CCWWGG	3 cut(s)	727, 1236, 1754
EcoT38I	GRGCYC	3 cut(s)	14, 166, 246
ErhI	CCWWGG	3 cut(s)	727, 1236, 1754
FaeI	CATG	5 cut(s)	64, 496, 563, 1302, 1690
FalI	AAGNNNNNCTT	2 cut(s)	633, 665
FaqI	GGGAC	7 cut(s)	178, 434, 472, 568, 773, 941, 1051
FatI	CATG	5 cut(s)	60, 492, 559, 1298, 1686
FauI	CCCGC	2 cut(s)	180, 409
FbaI	TGATCA	1 cut(s)	669
FblI	GTMKAC	2 cut(s)	585, 1851
Fnu4HI	GCNGC	9 cut(s)	107, 200, 540, 543, 989, 1321, 1590, 1964, 1967
FokI	GGATG	6 cut(s)	629, 778, 1013, 1280, 1542, 1574
FriOI	GRGCYC	3 cut(s)	14, 166, 246
Fsp4HI	GCNGC	9 cut(s)	107, 200, 540, 543, 989, 1321, 1590, 1964, 1967
FspBI	CTAG	6 cut(s)	866, 1152, 1251, 1326, 1755, 1893
GlaI	GCGC	2 cut(s)	699, 1312
GluI	GCNGC	9 cut(s)	107, 200, 540, 543, 989, 1321, 1590, 1964, 1967
GsaI	CCCAGC	6 cut(s)	169, 199, 378, 539, 760, 1072
HaeIII	GGCC	9 cut(s)	25, 38, 113, 326, 476, 515, 901, 1131, 1614
HapII	CCGG	5 cut(s)	110, 159, 208, 1063, 1764
HgaI	GACGC	1 cut(s)	121
HhaI	GCGC	2 cut(s)	700, 1313
Hin1I	GRCGYC	1 cut(s)	132
Hin1II	CATG	5 cut(s)	64, 496, 563, 1302, 1690
Hin6I	GCGC	2 cut(s)	698, 1311
HinP1I	GCGC	2 cut(s)	698, 1311
HindIII	AAGCTT	1 cut(s)	1425
HpaII	CCGG	5 cut(s)	110, 159, 208, 1063, 1764
HphI	GGTGA	3 cut(s)	1068, 1156, 1487
Hpy166II	GTNNAC	4 cut(s)	282, 586, 842, 1852
Hpy188I	TCNGA	6 cut(s)	30, 118, 384, 396, 826, 1842
Hpy188III	TCNNGA	7 cut(s)	61, 457, 680, 737, 866, 1027, 1697
Hpy8I	GTNNAC	4 cut(s)	282, 586, 842, 1852
Hpy99I	CGWCG	3 cut(s)	251, 332, 1846
HpyAV	CCTTC	5 cut(s)	82, 507, 787, 1694, 1741
HpyF10VI	GCNNNNNNNGC	7 cut(s)	229, 314, 323, 1078, 1254, 1308, 1317
HpyF3I	CTNAG	2 cut(s)	678, 1434
Hsp92I	GRCGYC	1 cut(s)	132
Hsp92II	CATG	5 cut(s)	64, 496, 563, 1302, 1690
HspAI	GCGC	2 cut(s)	698, 1311
KflI	GGGWCCC	2 cut(s)	459, 555
KroI	GCCGGC	1 cut(s)	109
KroNI	GCCGGC	1 cut(s)	111
Ksp22I	TGATCA	1 cut(s)	669
LmnI	GCTCC	6 cut(s)	17, 106, 161, 174, 1241, 1733
Lsp1109I	GCAGC	5 cut(s)	211, 529, 1307, 1576, 1950
LweI	GCATC	3 cut(s)	423, 991, 1244
MaeI	CTAG	6 cut(s)	866, 1152, 1251, 1326, 1755, 1893
MaeIII	GTNAC	3 cut(s)	703, 1543, 1649
MboII	GAAGA	7 cut(s)	38, 127, 632, 782, 813, 1543, 1810
MfeI	CAATTG	1 cut(s)	1555
MflI	RGATCY	2 cut(s)	646, 1750
MhlI	GDGCHC	7 cut(s)	14, 166, 225, 234, 246, 1764, 1874
MlsI	TGGCCA	1 cut(s)	515
MluNI	TGGCCA	1 cut(s)	515
MlyI	GAGTC	5 cut(s)	113, 224, 692, 1156, 1295
MmeI	TCCRAC	3 cut(s)	141, 947, 1865
Mox20I	TGGCCA	1 cut(s)	515
MroNI	GCCGGC	1 cut(s)	109
MroXI	GAANNNNTTC	1 cut(s)	1386
MscI	TGGCCA	1 cut(s)	515
MseI	TTAA	6 cut(s)	408, 597, 1041, 1440, 1497, 1857
MslI	CAYNNNNRTG	1 cut(s)	611
Msp20I	TGGCCA	1 cut(s)	515
MspA1I	CMGCKG	2 cut(s)	539, 1206
MspI	CCGG	5 cut(s)	110, 159, 208, 1063, 1764
MspR9I	CCNGG	8 cut(s)	16, 345, 443, 1142, 1617, 1764, 1765, 1809
MunI	CAATTG	1 cut(s)	1555
Mva1269I	GAATGC	1 cut(s)	339
MvaI	CCWGG	6 cut(s)	16, 345, 443, 1142, 1617, 1809
MwoI	GCNNNNNNNGC	7 cut(s)	229, 314, 323, 1078, 1254, 1308, 1317
NaeI	GCCGGC	1 cut(s)	111
NciI	CCSGG	2 cut(s)	1764, 1765
NgoMIV	GCCGGC	1 cut(s)	109
NlaIII	CATG	5 cut(s)	64, 496, 563, 1302, 1690
NmuCI	GTSAC	2 cut(s)	1543, 1649
PaeR7I	CTCGAG	1 cut(s)	7
PagI	TCATGA	1 cut(s)	60
PcsI	WCGNNNNNNNCGW	1 cut(s)	246
PctI	GAATGC	1 cut(s)	339
PdiI	GCCGGC	1 cut(s)	111
PdmI	GAANNNNTTC	1 cut(s)	1386
PfeI	GAWTC	6 cut(s)	525, 818, 1030, 1095, 1382, 1812
Pfl23II	CGTACG	1 cut(s)	144
PflMI	CCANNNNNTGG	1 cut(s)	21
PfoI	TCCNGGA	2 cut(s)	14, 1807
PinAI	ACCGGT	2 cut(s)	207, 1062
PkrI	GCNGC	9 cut(s)	108, 201, 541, 544, 990, 1322, 1591, 1965, 1968
PleI	GAGTC	5 cut(s)	113, 223, 691, 1155, 1294
PpsI	GAGTC	5 cut(s)	113, 223, 691, 1155, 1294
PpuMI	RGGWCCY	2 cut(s)	459, 555
PsiI	TTATAA	1 cut(s)	1122
Psp124BI	GAGCTC	1 cut(s)	14
Psp5II	RGGWCCY	2 cut(s)	459, 555
Psp6I	CCWGG	6 cut(s)	14, 343, 441, 1140, 1615, 1807
PspFI	CCCAGC	6 cut(s)	165, 195, 374, 535, 756, 1068
PspGI	CCWGG	6 cut(s)	14, 343, 441, 1140, 1615, 1807
PspLI	CGTACG	1 cut(s)	144
PspPI	GGNCC	9 cut(s)	37, 439, 459, 475, 555, 955, 1065, 1130, 1613
PspPPI	RGGWCCY	2 cut(s)	459, 555
PspXI	VCTCGAGB	1 cut(s)	7
PstI	CTGCAG	3 cut(s)	1198, 1594, 1968
PstNI	CAGNNNCTG	3 cut(s)	21, 542, 1616
PsuI	RGATCY	2 cut(s)	646, 1750
PvuII	CAGCTG	1 cut(s)	1206
RsaI	GTAC	7 cut(s)	146, 296, 676, 850, 979, 1596, 1634
RsaNI	GTAC	7 cut(s)	145, 295, 675, 849, 978, 1595, 1633
RseI	CAYNNNNRTG	1 cut(s)	611
SacI	GAGCTC	1 cut(s)	14
SaqAI	TTAA	6 cut(s)	408, 597, 1041, 1440, 1497, 1857
SatI	GCNGC	9 cut(s)	107, 200, 540, 543, 989, 1321, 1590, 1964, 1967
Sau96I	GGNCC	9 cut(s)	37, 439, 459, 475, 555, 955, 1065, 1130, 1613
ScaI	AGTACT	2 cut(s)	296, 676
SchI	GAGTC	5 cut(s)	113, 224, 692, 1156, 1295
ScrFI	CCNGG	8 cut(s)	16, 345, 443, 1142, 1617, 1764, 1765, 1809
SduI	GDGCHC	7 cut(s)	14, 166, 225, 234, 246, 1764, 1874
SfaNI	GCATC	3 cut(s)	423, 991, 1244
SfcI	CTRYAG	3 cut(s)	1194, 1590, 1964
Sfr274I	CTCGAG	1 cut(s)	7
SinI	GGWCC	5 cut(s)	439, 459, 555, 955, 1065
SlaI	CTCGAG	1 cut(s)	7
SmaI	CCCGGG	1 cut(s)	1765
SmiMI	CAYNNNNRTG	1 cut(s)	611
SmlI	CTYRAG	2 cut(s)	7, 254
SmoI	CTYRAG	2 cut(s)	7, 254
SspI	AATATT	1 cut(s)	1225
SspMI	CTAG	6 cut(s)	866, 1152, 1251, 1326, 1755, 1893
SstI	GAGCTC	1 cut(s)	14
StyD4I	CCNGG	8 cut(s)	14, 343, 441, 1140, 1615, 1762, 1763, 1807
StyI	CCWWGG	3 cut(s)	727, 1236, 1754
TaqI	TCGA	5 cut(s)	8, 213, 246, 1339, 1698
TaqII	GACCGA	2 cut(s)	404, 1182
TatI	WGTACW	3 cut(s)	294, 674, 1594
TauI	GCSGC	3 cut(s)	109, 542, 991
TfiI	GAWTC	6 cut(s)	525, 818, 1030, 1095, 1382, 1812
Tru1I	TTAA	6 cut(s)	408, 597, 1041, 1440, 1497, 1857
Tru9I	TTAA	6 cut(s)	408, 597, 1041, 1440, 1497, 1857
TscAI	CASTG	4 cut(s)	616, 1188, 1785, 1903
TseFI	GTSAC	2 cut(s)	1543, 1649
TseI	GCWGC	6 cut(s)	199, 542, 1320, 1589, 1963, 1966
Tsp45I	GTSAC	2 cut(s)	1543, 1649
TspDTI	ATGAA	8 cut(s)	49, 576, 633, 769, 806, 1008, 1578, 1675
TspMI	CCCGGG	1 cut(s)	1763
TspRI	CASTG	4 cut(s)	616, 1188, 1785, 1903
Van91I	CCANNNNNTGG	1 cut(s)	21
VpaK11BI	GGWCC	5 cut(s)	439, 459, 555, 955, 1065
XapI	RAATTY	2 cut(s)	593, 1186
XbaI	TCTAGA	1 cut(s)	865
XcmI	CCANNNNNNNNNTGG	3 cut(s)	319, 508, 725
XhoI	CTCGAG	1 cut(s)	7
XmaI	CCCGGG	1 cut(s)	1763
XmaJI	CCTAGG	1 cut(s)	1754
XmiI	GTMKAC	2 cut(s)	585, 1851
XmnI	GAANNNNTTC	1 cut(s)	1386
XspI	CTAG	6 cut(s)	866, 1152, 1251, 1326, 1755, 1893
ZrmI	AGTACT	2 cut(s)	296, 676

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.