Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0012427

Physical Location & Seq

Genomic Coordinates Forward (+)

12886314 .. 12888926

Gene Structure 2613 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1159300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1998 bp

ATGGACTCGGGCTCCAGGAACTGGCCTCCGATCATGGCCCTCAATCTTCTCATTATCCTCACGATCATGCCCTTCTCACTCGCCCAGCCAACCGCCGCAGCTCCGGCGCCGGCCTCCGACTCGTGCAACGGCGTCTTCCTGTCCTACGCCTATACTGCCGGACCCCAGCTCCCGCCCGAGGTCAAAGACCCGAAACAGCAGCCTTACAAGTTCGAGTCGGTTCTCACAGTGCTCAACAACGGGCTCGACGATCTCAAGTCGTGGAGGGTGTTTGTGGGGTTTACCAACAACGAGTACTTGGTCTCTGCCTCCAATGCTGTTTTGGCCGACGGTACTAGTATCCCTGGGGGTGTCGGAAACGGCACCGTTTTTGCTGGGTATCCGATGACCGATCTGAAGACGGCGGTTAAGACGGCGGGGGACTTGGCCCAGATGCAGGTTCAGGTCAAATTGATCGGGACCCAGTTCGGTGTGGCCCCACCCAAAGTTCCCATGCCTTCCAATATTACTCTGGCCAATGATGGGTTCGTCTGCCCAGCGGCTGCAATGCAAGGTGAGCCCATTAGATTTAGCTGTTGGACCCATGAAATGCAAGTCTGTTGCACCGTAGACGCAAATTTTAAAACGAACATCACTGTGGACGATGAGTTCCTCCCCCGTCAAAAGGGAGATCTTACAATGATGTATGATGTGATCAGTACTCGGGCAACAGATTACACGGCGCAAGTGACAATCGCCAACCATAACTCCCTTGGTCGTCTTGATAATTGGAAACTGAGCTGGGACTGGATGGAAGATGAGTTCATATTTTCAACGAAGGGGGCTTATCCATCTGTTGTTGATTCTTCTGATTGTATATTCGGTAAACAAGGTACATTCTACGAGAATCTAGACTTCTCAACTGTATTGAATTGTGAAAGACGGCCAACAATTATTGACCTTCCTCCAACAAAAGCCAATGACACACTTCTTGGTCTGGTCCCTTATTGTTGCCGAAATGGTACTATCTTGCCACCAACAATGGATGCAAGCAAGTCAGTTTCATCATTCCAGATGCAAGTTTTTAAAATGCCTCCAAATCTCAACCGGTCCCAGCTCTCACCGCCGCAAAACTGGGGAATCAAAGGCACGCTCAACCCTGATTATAAATGTGGCCCTCCTGTCCGGGTGAGTCCCAGTCAGTTCCCTGACCGATCTGGCTTGCCAGTGAATATATCTGCAGTAGCCAGCTGGCAGGTTGTGTGCAATATTACCCAACCCAGAGGAGCAATCCCTAGTTGCTGTGTTTCATTTTCTGCTTTCTACAATGAGTCTGTCGTCCCATGCAACACTTGCGCTTGTGGCTGCCCTAGTAATACAGTTCGAACTTGTAGCACAACTGCACCAGCTATGCTTCTCCCACCAGAGTCACTTCTTGTTCCCTTTGACAACCGAACTGTCAAGGCAAAATCTTGGGCTGATATTAAACACCTTCCAGTCCCAAACCCGACACCTTGTGGTGATAACTGTGGGATCAGCATCAACTGGCATTTATATACAGACTACTCTCGTGGATGGAGTGCAAGGGTCACAGTCTTCAATTGGGATGAAACGGCTTTTGTTGATTGGTTCGCTGCAGTACAACTGGATAAAGCAGGCCCTGGTTTCGAAAAGGCGTACTCTTTCAACGCAAGTCACTTGGAAATTAATGGTGTCAATAATACCGTATTCATGCAAGGCTTCAAAGGATTGAACTATCTTGTGGCGGAAACAGATGGAGCCAACCCAAAGAAGGATCCTAGGGTGCCTGGGAAACAGCAGTCAGTGCTCTCATTTACAAAGAAGATTACTCCCGGAATCAATTTGGTTGGTGGAGATGGGTTTCCGACGAAAGTATACTTTAACGGGGAGGAGTGCGCTCTTCCTACAGCATATCCTAGCAGTGGTTACAGAAAACGCACATCTCTAATTTTCTCAGTTCTCGTAATGGTTGTAGCGTTTATGGTGATGCAGCAGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005886 GO:0005911 GO:0007275 GO:0008150 GO:0009506 GO:0009653 GO:0009826 GO:0009888 GO:0009932 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0021700 GO:0022622 GO:0030054 GO:0030154 GO:0031224 GO:0031225 GO:0031226 GO:0031410 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0032989 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046658 GO:0048364 GO:0048468 GO:0048469 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048856 GO:0048869 GO:0055044 GO:0060560 GO:0071695 GO:0071840 GO:0071944 GO:0090558 GO:0090627 GO:0097708 GO:0098791 GO:0099402 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

COBRA

Protein Analysis

666

Amino Acids

72.49

Weight (kDa)

6.04

Isoelectric Point (pI)

36.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
COBRA	PF04833	239 - 418	3.1e-64	COBRA-like protein
COB_C	PF25079	426 - 639	2.9e-99	COBRA family C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012427)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G16120
fragaria_vesca	FvH4_4g19240
malus_domestica	MD13G1159600.v1.1 MD16G1159300.v1.1
prunus_persica	Prupe.1G126300_v2.0.a1
pyrus_communis	pycom13g13600 pycom16g13430
rosa_chinensis	RchiOBHm_Chr4g0424221
rosa_laevigata	RLG00000007495
rosa_multiflora	Rmu_sc0000980.1_g000001 Rmu_sc0002260.1_g000034 Rmu_sc0019001.1_g000001
rosa_roxburghii	Rroxscaffold_5G00366170
rosa_rugosa	Rorug04G0194400
rosa_samantha	Rh4AG251800 Rh4BG257100 Rh4CG267700 Rh4DG251500
rosa_wichuraiana	Rw4G021710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1146
Acc36I	ACCTGC	2 cut(s)	427, 1225
AccB1I	GGYRCC	3 cut(s)	106, 362, 1783
AccB7I	CCANNNNNTGG	1 cut(s)	21
AccI	GTMKAC	2 cut(s)	609, 1875
AciI	CCGC	9 cut(s)	93, 96, 173, 404, 416, 539, 1103, 1106, 1745
AclWI	GGATC	3 cut(s)	1520, 1769, 1782
AcoI	YGGCCR	3 cut(s)	324, 513, 923
AcsI	RAATTY	1 cut(s)	616
AcuI	CTGAAG	1 cut(s)	416
AcyI	GRCGYC	2 cut(s)	107, 132
AdeI	CACNNNGTG	1 cut(s)	1496
AfaI	GTAC	7 cut(s)	296, 334, 700, 874, 1003, 1620, 1658
AfiI	CCNNNNNNNGG	7 cut(s)	21, 178, 436, 522, 664, 1771, 1922
AgeI	ACCGGT	1 cut(s)	1086
AgsI	TTSAA	6 cut(s)	813, 910, 1579, 1666, 1723, 1732
AhlI	ACTAGT	1 cut(s)	335
AjnI	CCWGG	4 cut(s)	14, 343, 1639, 1786
AjuI	GAANNNNNNNTTGG	2 cut(s)	509, 541
AloI	GAACNNNNNNTCC	4 cut(s)	632, 664, 785, 817
AluBI	AGCT	7 cut(s)	101, 169, 573, 780, 1096, 1230, 1388
AluI	AGCT	7 cut(s)	101, 169, 573, 780, 1096, 1230, 1388
Alw21I	GWGCWC	2 cut(s)	234, 1809
Alw26I	GTCTC	1 cut(s)	307
AlwI	GGATC	3 cut(s)	1520, 1769, 1782
AlwNI	CAGNNNCTG	3 cut(s)	21, 542, 1640
Ama87I	CYCGRG	3 cut(s)	7, 176, 702
ApeKI	GCWGC	6 cut(s)	98, 199, 542, 1344, 1613, 1990
ApoI	RAATTY	1 cut(s)	616
AseI	ATTAAT	1 cut(s)	1686
AsiGI	ACCGGT	1 cut(s)	1086
AspA2I	CCTAGG	1 cut(s)	1778
AspLEI	GCGC	4 cut(s)	109, 724, 1337, 1898
AsuC2I	CCSGG	2 cut(s)	1166, 1833
AsuHPI	GGTGA	5 cut(s)	566, 1092, 1180, 1511, 1996
AsuII	TTCGAA	2 cut(s)	1363, 1647
AvaI	CYCGRG	3 cut(s)	7, 176, 702
AvaII	GGWCC	5 cut(s)	161, 459, 579, 979, 1089
AvrII	CCTAGG	1 cut(s)	1778
BalI	TGGCCA	1 cut(s)	515
BamHI	GGATCC	1 cut(s)	1774
BanI	GGYRCC	3 cut(s)	106, 362, 1783
BanII	GRGCYC	3 cut(s)	14, 246, 561
BauI	CACGAG	2 cut(s)	121, 1548
BbsI	GAAGAC	3 cut(s)	127, 404, 1567
Bbv12I	GWGCWC	2 cut(s)	234, 1809
BbvI	GCAGC	5 cut(s)	110, 211, 529, 1331, 1600
BccI	CCATC	6 cut(s)	515, 784, 838, 1548, 1748, 1850
BceAI	ACGGC	7 cut(s)	145, 376, 417, 429, 735, 938, 1608
BciT130I	CCWGG	4 cut(s)	16, 345, 1641, 1788
BciVI	GTATCC	2 cut(s)	350, 390
BclI	TGATCA	1 cut(s)	693
BcnI	CCSGG	2 cut(s)	1166, 1833
BcoDI	GTCTC	1 cut(s)	307
BcuI	ACTAGT	1 cut(s)	335
BfaI	CTAG	6 cut(s)	336, 890, 1275, 1350, 1779, 1917
BfmI	CTRYAG	3 cut(s)	1218, 1614, 1905
BfoI	RGCGCY	1 cut(s)	110
BfuAI	ACCTGC	2 cut(s)	427, 1225
BfuI	GTATCC	2 cut(s)	350, 390
BglII	AGATCT	1 cut(s)	670
BisI	GCNGC	9 cut(s)	96, 99, 200, 540, 543, 1106, 1345, 1614, 1991
BlnI	CCTAGG	1 cut(s)	1778
BlsI	GCNGC	9 cut(s)	97, 100, 201, 541, 544, 1107, 1346, 1615, 1992
BmcAI	AGTACT	2 cut(s)	296, 700
Bme1390I	CCNGG	6 cut(s)	16, 345, 1166, 1641, 1788, 1833
Bme18I	GGWCC	5 cut(s)	161, 459, 579, 979, 1089
BmeT110I	CYCGRG	3 cut(s)	7, 176, 702
BmrFI	CCNGG	6 cut(s)	16, 345, 1166, 1641, 1788, 1833
BmrI	ACTGGG	3 cut(s)	457, 1123, 1170
BmsI	GCATC	5 cut(s)	423, 1015, 1044, 1527, 1977
BmuI	ACTGGG	3 cut(s)	457, 1123, 1170
BpiI	GAAGAC	3 cut(s)	127, 404, 1567
Bpu14I	TTCGAA	2 cut(s)	1363, 1647
BpuEI	CTTGAG	1 cut(s)	239
BpuMI	CCSGG	2 cut(s)	1166, 1833
BsaBI	GATNNNNATC	1 cut(s)	1517
BsaHI	GRCGYC	2 cut(s)	107, 132
BsaI	GGTCTC	1 cut(s)	307
BsaJI	CCNNGG	7 cut(s)	177, 343, 344, 751, 1639, 1778, 1787
BsaWI	WCCGGW	1 cut(s)	1086
BsaXI	ACNNNNNCTCC	5 cut(s)	26, 153, 183, 731, 761
Bsc4I	CCNNNNNNNGG	7 cut(s)	21, 178, 436, 522, 664, 1771, 1922
Bse118I	RCCGGY	2 cut(s)	109, 1086
Bse1I	ACTGG	9 cut(s)	26, 463, 791, 1118, 1176, 1205, 1475, 1529, 1629
Bse3DI	GCAATG	1 cut(s)	552
Bse8I	GATNNNNATC	1 cut(s)	1517
BseBI	CCWGG	4 cut(s)	16, 345, 1641, 1788
BseDI	CCNNGG	7 cut(s)	177, 343, 344, 751, 1639, 1778, 1787
BseGI	GGATG	4 cut(s)	795, 1030, 1559, 1591
BseJI	GATNNNNATC	1 cut(s)	1517
BseLI	CCNNNNNNNGG	7 cut(s)	21, 178, 436, 522, 664, 1771, 1922
BseMI	GCAATG	1 cut(s)	552
BseMII	CTCAG	2 cut(s)	767, 1968
BseNI	ACTGG	9 cut(s)	26, 463, 791, 1118, 1176, 1205, 1475, 1529, 1629
BseRI	GAGGAG	2 cut(s)	1278, 1904
BseXI	GCAGC	5 cut(s)	110, 211, 529, 1331, 1600
BseYI	CCCAGC	6 cut(s)	84, 165, 374, 535, 780, 1092
BsgI	GTGCAG	1 cut(s)	1365
BshNI	GGYRCC	3 cut(s)	106, 362, 1783
BshTI	ACCGGT	1 cut(s)	1086
BsiHKAI	GWGCWC	2 cut(s)	234, 1809
BsiHKCI	CYCGRG	3 cut(s)	7, 176, 702
BsiSI	CCGG	6 cut(s)	104, 110, 159, 1087, 1165, 1833
BslFI	GGGAC	8 cut(s)	434, 472, 797, 965, 1075, 1158, 1304, 1463
BslI	CCNNNNNNNGG	7 cut(s)	21, 178, 436, 522, 664, 1771, 1922
BsmAI	GTCTC	1 cut(s)	307
BsmFI	GGGAC	8 cut(s)	434, 472, 797, 965, 1075, 1158, 1304, 1463
Bso31I	GGTCTC	1 cut(s)	307
BsoBI	CYCGRG	3 cut(s)	7, 176, 702
Bsp119I	TTCGAA	2 cut(s)	1363, 1647
Bsp1286I	GDGCHC	5 cut(s)	14, 234, 246, 561, 1809
BspACI	CCGC	9 cut(s)	93, 96, 173, 404, 416, 539, 1103, 1106, 1745
BspCNI	CTCAG	2 cut(s)	768, 1967
BspMAI	CTGCAG	2 cut(s)	1222, 1618
BspMI	ACCTGC	2 cut(s)	427, 1225
BspPI	GGATC	3 cut(s)	1520, 1769, 1782
BspQI	GCTCTTC	1 cut(s)	1905
BspT104I	TTCGAA	2 cut(s)	1363, 1647
BspT107I	GGYRCC	3 cut(s)	106, 362, 1783
BspTNI	GGTCTC	1 cut(s)	307
BsrDI	GCAATG	1 cut(s)	552
BsrFI	RCCGGY	2 cut(s)	109, 1086
BsrI	ACTGG	9 cut(s)	26, 463, 791, 1118, 1176, 1205, 1475, 1529, 1629
BssAI	RCCGGY	2 cut(s)	109, 1086
BssECI	CCNNGG	7 cut(s)	177, 343, 344, 751, 1639, 1778, 1787
BssNAI	GTATAC	1 cut(s)	1876
BssNI	GRCGYC	2 cut(s)	107, 132
BssSI	CACGAG	2 cut(s)	121, 1548
BssT1I	CCWWGG	2 cut(s)	751, 1778
Bst1107I	GTATAC	1 cut(s)	1876
Bst2BI	CACGAG	2 cut(s)	121, 1548
Bst2UI	CCWGG	4 cut(s)	16, 345, 1641, 1788
Bst6I	CTCTTC	1 cut(s)	1905
BstACI	GRCGYC	2 cut(s)	107, 132
BstAPI	GCANNNNNTGC	2 cut(s)	1332, 1804
BstBI	TTCGAA	2 cut(s)	1363, 1647
BstC8I	GCNNGC	7 cut(s)	111, 1030, 1130, 1202, 1228, 1232, 1636
BstDEI	CTNAG	2 cut(s)	776, 1954
BstF5I	GGATG	4 cut(s)	795, 1030, 1559, 1591
BstH2I	RGCGCY	1 cut(s)	110
BstHHI	GCGC	4 cut(s)	109, 724, 1337, 1898
BstMAI	GTCTC	1 cut(s)	307
BstMWI	GCNNNNNNNGC	9 cut(s)	104, 155, 314, 323, 556, 1102, 1332, 1341, 1804
BstNI	CCWGG	4 cut(s)	16, 345, 1641, 1788
BstSCI	CCNGG	6 cut(s)	14, 343, 1164, 1639, 1786, 1831
BstSFI	CTRYAG	3 cut(s)	1218, 1614, 1905
BstV1I	GCAGC	5 cut(s)	110, 211, 529, 1331, 1600
BstV2I	GAAGAC	3 cut(s)	127, 404, 1567
BstX2I	RGATCY	2 cut(s)	670, 1774
BstYI	RGATCY	2 cut(s)	670, 1774
BstZ17I	GTATAC	1 cut(s)	1876
BsuI	GTATCC	2 cut(s)	350, 390
BtsCI	GGATG	4 cut(s)	795, 1030, 1559, 1591
BtsI	GCAGTG	1 cut(s)	1927
BtsIMutI	CAGTG	5 cut(s)	234, 633, 1212, 1809, 1927
BveI	ACCTGC	2 cut(s)	427, 1225
Cac8I	GCNNGC	7 cut(s)	111, 1030, 1130, 1202, 1228, 1232, 1636
CaiI	CAGNNNCTG	3 cut(s)	21, 542, 1640
CfoI	GCGC	4 cut(s)	109, 724, 1337, 1898
Cfr10I	RCCGGY	2 cut(s)	109, 1086
CseI	GACGC	2 cut(s)	121, 620
Csp6I	GTAC	7 cut(s)	295, 333, 699, 873, 1002, 1619, 1657
CspAI	ACCGGT	1 cut(s)	1086
CviAII	CATG	6 cut(s)	34, 67, 493, 584, 1323, 1711
CviQI	GTAC	7 cut(s)	295, 333, 699, 873, 1002, 1619, 1657
DdeI	CTNAG	2 cut(s)	776, 1954
DinI	GGCGCC	1 cut(s)	108
DraI	TTTAAA	2 cut(s)	622, 1066
DraIII	CACNNNGTG	1 cut(s)	1496
EaeI	YGGCCR	3 cut(s)	324, 513, 923
Eam1104I	CTCTTC	1 cut(s)	1905
EarI	CTCTTC	1 cut(s)	1905
EciI	GGCGGA	1 cut(s)	1760
Eco130I	CCWWGG	2 cut(s)	751, 1778
Eco24I	GRGCYC	3 cut(s)	14, 246, 561
Eco31I	GGTCTC	1 cut(s)	307
Eco47I	GGWCC	5 cut(s)	161, 459, 579, 979, 1089
Eco57I	CTGAAG	1 cut(s)	416
Eco88I	CYCGRG	3 cut(s)	7, 176, 702
EcoO109I	RGGNCCY	2 cut(s)	459, 1637
EcoRII	CCWGG	4 cut(s)	14, 343, 1639, 1786
EcoT14I	CCWWGG	2 cut(s)	751, 1778
EcoT38I	GRGCYC	3 cut(s)	14, 246, 561
EgeI	GGCGCC	1 cut(s)	108
EheI	GGCGCC	1 cut(s)	108
ErhI	CCWWGG	2 cut(s)	751, 1778
FaeI	CATG	6 cut(s)	37, 70, 496, 587, 1326, 1714
FaqI	GGGAC	8 cut(s)	434, 472, 797, 965, 1075, 1158, 1304, 1463
FatI	CATG	6 cut(s)	33, 66, 492, 583, 1322, 1710
FauI	CCCGC	2 cut(s)	180, 409
FbaI	TGATCA	1 cut(s)	693
FblI	GTMKAC	2 cut(s)	609, 1875
Fnu4HI	GCNGC	9 cut(s)	96, 99, 200, 540, 543, 1106, 1345, 1614, 1991
FokI	GGATG	4 cut(s)	802, 1037, 1566, 1598
FriOI	GRGCYC	3 cut(s)	14, 246, 561
Fsp4HI	GCNGC	9 cut(s)	96, 99, 200, 540, 543, 1106, 1345, 1614, 1991
FspBI	CTAG	6 cut(s)	336, 890, 1275, 1350, 1779, 1917
GlaI	GCGC	4 cut(s)	108, 723, 1336, 1897
GluI	GCNGC	9 cut(s)	96, 99, 200, 540, 543, 1106, 1345, 1614, 1991
GsaI	CCCAGC	6 cut(s)	88, 169, 378, 539, 784, 1096
HaeII	RGCGCY	1 cut(s)	110
HapII	CCGG	6 cut(s)	104, 110, 159, 1087, 1165, 1833
HgaI	GACGC	2 cut(s)	121, 620
HhaI	GCGC	4 cut(s)	109, 724, 1337, 1898
Hin1I	GRCGYC	2 cut(s)	107, 132
Hin1II	CATG	6 cut(s)	37, 70, 496, 587, 1326, 1714
Hin6I	GCGC	4 cut(s)	107, 722, 1335, 1896
HinP1I	GCGC	4 cut(s)	107, 722, 1335, 1896
HpaII	CCGG	6 cut(s)	104, 110, 159, 1087, 1165, 1833
HphI	GGTGA	5 cut(s)	566, 1092, 1180, 1511, 1996
Hpy166II	GTNNAC	5 cut(s)	282, 610, 640, 866, 1876
Hpy188I	TCNGA	7 cut(s)	30, 118, 356, 384, 396, 850, 1866
Hpy188III	TCNNGA	5 cut(s)	61, 457, 761, 890, 1051
Hpy8I	GTNNAC	5 cut(s)	282, 610, 640, 866, 1876
Hpy99I	CGWCG	3 cut(s)	251, 332, 1870
HpyAV	CCTTC	6 cut(s)	82, 507, 811, 950, 1481, 1765
HpyF10VI	GCNNNNNNNGC	9 cut(s)	104, 155, 314, 323, 556, 1102, 1332, 1341, 1804
HpyF3I	CTNAG	2 cut(s)	776, 1954
Hsp92I	GRCGYC	2 cut(s)	107, 132
Hsp92II	CATG	6 cut(s)	37, 70, 496, 587, 1326, 1714
HspAI	GCGC	4 cut(s)	107, 722, 1335, 1896
KasI	GGCGCC	1 cut(s)	106
KflI	GGGWCCC	1 cut(s)	459
KroI	GCCGGC	1 cut(s)	109
KroNI	GCCGGC	1 cut(s)	111
Ksp22I	TGATCA	1 cut(s)	693
LguI	GCTCTTC	1 cut(s)	1905
LmnI	GCTCC	5 cut(s)	17, 106, 174, 1265, 1757
Lsp1109I	GCAGC	5 cut(s)	110, 211, 529, 1331, 1600
LweI	GCATC	5 cut(s)	423, 1015, 1044, 1527, 1977
MaeI	CTAG	6 cut(s)	336, 890, 1275, 1350, 1779, 1917
MaeIII	GTNAC	5 cut(s)	727, 1407, 1567, 1673, 1925
MboII	GAAGA	8 cut(s)	38, 127, 409, 806, 837, 1567, 1834, 1892
MfeI	CAATTG	1 cut(s)	1579
MflI	RGATCY	2 cut(s)	670, 1774
MhlI	GDGCHC	5 cut(s)	14, 234, 246, 561, 1809
MlsI	TGGCCA	1 cut(s)	515
MluCI	AATT	9 cut(s)	449, 616, 766, 910, 930, 1579, 1683, 1840, 1947
MluNI	TGGCCA	1 cut(s)	515
Mly113I	GGCGCC	1 cut(s)	107
MlyI	GAGTC	5 cut(s)	113, 224, 1180, 1319, 1415
MmeI	TCCRAC	5 cut(s)	141, 334, 557, 971, 1889
Mox20I	TGGCCA	1 cut(s)	515
MroNI	GCCGGC	1 cut(s)	109
MscI	TGGCCA	1 cut(s)	515
MseI	TTAA	6 cut(s)	408, 621, 1065, 1464, 1686, 1881
MslI	CAYNNNNRTG	2 cut(s)	65, 635
Msp20I	TGGCCA	1 cut(s)	515
MspA1I	CMGCKG	2 cut(s)	539, 1230
MspI	CCGG	6 cut(s)	104, 110, 159, 1087, 1165, 1833
MspR9I	CCNGG	6 cut(s)	16, 345, 1166, 1641, 1788, 1833
MunI	CAATTG	1 cut(s)	1579
MvaI	CCWGG	4 cut(s)	16, 345, 1641, 1788
MwoI	GCNNNNNNNGC	9 cut(s)	104, 155, 314, 323, 556, 1102, 1332, 1341, 1804
NaeI	GCCGGC	1 cut(s)	111
NarI	GGCGCC	1 cut(s)	107
NciI	CCSGG	2 cut(s)	1166, 1833
NgoMIV	GCCGGC	1 cut(s)	109
NlaIII	CATG	6 cut(s)	37, 70, 496, 587, 1326, 1714
NmuCI	GTSAC	4 cut(s)	727, 1407, 1567, 1673
NspV	TTCGAA	2 cut(s)	1363, 1647
PasI	CCCWGGG	1 cut(s)	344
PciSI	GCTCTTC	1 cut(s)	1905
PcsI	WCGNNNNNNNCGW	1 cut(s)	246
PdiI	GCCGGC	1 cut(s)	111
PfeI	GAWTC	4 cut(s)	842, 886, 1119, 1836
PflMI	CCANNNNNTGG	1 cut(s)	21
PfoI	TCCNGGA	2 cut(s)	14, 1831
PinAI	ACCGGT	1 cut(s)	1086
PkrI	GCNGC	9 cut(s)	97, 100, 201, 541, 544, 1107, 1346, 1615, 1992
PleI	GAGTC	5 cut(s)	113, 223, 1179, 1318, 1414
PluTI	GGCGCC	1 cut(s)	110
PpsI	GAGTC	5 cut(s)	113, 223, 1179, 1318, 1414
PpuMI	RGGWCCY	1 cut(s)	459
PshBI	ATTAAT	1 cut(s)	1686
PsiI	TTATAA	1 cut(s)	1146
Psp5II	RGGWCCY	1 cut(s)	459
Psp6I	CCWGG	4 cut(s)	14, 343, 1639, 1786
PspFI	CCCAGC	6 cut(s)	84, 165, 374, 535, 780, 1092
PspGI	CCWGG	4 cut(s)	14, 343, 1639, 1786
PspPPI	RGGWCCY	1 cut(s)	459
PstI	CTGCAG	2 cut(s)	1222, 1618
PstNI	CAGNNNCTG	3 cut(s)	21, 542, 1640
PsuI	RGATCY	2 cut(s)	670, 1774
PvuII	CAGCTG	1 cut(s)	1230
RsaI	GTAC	7 cut(s)	296, 334, 700, 874, 1003, 1620, 1658
RsaNI	GTAC	7 cut(s)	295, 333, 699, 873, 1002, 1619, 1657
RseI	CAYNNNNRTG	2 cut(s)	65, 635
SapI	GCTCTTC	1 cut(s)	1905
SaqAI	TTAA	6 cut(s)	408, 621, 1065, 1464, 1686, 1881
SatI	GCNGC	9 cut(s)	96, 99, 200, 540, 543, 1106, 1345, 1614, 1991
ScaI	AGTACT	2 cut(s)	296, 700
SchI	GAGTC	5 cut(s)	113, 224, 1180, 1319, 1415
ScrFI	CCNGG	6 cut(s)	16, 345, 1166, 1641, 1788, 1833
SduI	GDGCHC	5 cut(s)	14, 234, 246, 561, 1809
SfaNI	GCATC	5 cut(s)	423, 1015, 1044, 1527, 1977
SfcI	CTRYAG	3 cut(s)	1218, 1614, 1905
SfoI	GGCGCC	1 cut(s)	108
SfuI	TTCGAA	2 cut(s)	1363, 1647
SinI	GGWCC	5 cut(s)	161, 459, 579, 979, 1089
SmiMI	CAYNNNNRTG	2 cut(s)	65, 635
SmlI	CTYRAG	1 cut(s)	254
SmoI	CTYRAG	1 cut(s)	254
SpeI	ACTAGT	1 cut(s)	335
Sse9I	AATT	9 cut(s)	449, 616, 766, 910, 930, 1579, 1683, 1840, 1947
SsiI	CCGC	9 cut(s)	93, 96, 173, 404, 416, 539, 1103, 1106, 1745
SspDI	GGCGCC	1 cut(s)	106
SspI	AATATT	2 cut(s)	505, 1249
SspMI	CTAG	6 cut(s)	336, 890, 1275, 1350, 1779, 1917
StyD4I	CCNGG	6 cut(s)	14, 343, 1164, 1639, 1786, 1831
StyI	CCWWGG	2 cut(s)	751, 1778
TaqI	TCGA	4 cut(s)	213, 246, 1363, 1647
TaqII	GACCGA	2 cut(s)	404, 1206
TasI	AATT	9 cut(s)	449, 616, 766, 910, 930, 1579, 1683, 1840, 1947
TatI	WGTACW	3 cut(s)	294, 698, 1618
TauI	GCSGC	3 cut(s)	98, 542, 1108
TfiI	GAWTC	4 cut(s)	842, 886, 1119, 1836
Tru1I	TTAA	6 cut(s)	408, 621, 1065, 1464, 1686, 1881
Tru9I	TTAA	6 cut(s)	408, 621, 1065, 1464, 1686, 1881
TscAI	CASTG	5 cut(s)	234, 640, 1212, 1809, 1927
TseFI	GTSAC	4 cut(s)	727, 1407, 1567, 1673
TseI	GCWGC	6 cut(s)	98, 199, 542, 1344, 1613, 1990
Tsp45I	GTSAC	4 cut(s)	727, 1407, 1567, 1673
TspDTI	ATGAA	6 cut(s)	600, 793, 1032, 1278, 1602, 1699
TspRI	CASTG	5 cut(s)	234, 640, 1212, 1809, 1927
Van91I	CCANNNNNTGG	1 cut(s)	21
VpaK11BI	GGWCC	5 cut(s)	161, 459, 579, 979, 1089
VspI	ATTAAT	1 cut(s)	1686
XapI	RAATTY	1 cut(s)	616
XbaI	TCTAGA	1 cut(s)	889
XcmI	CCANNNNNNNNNTGG	3 cut(s)	319, 508, 749
XmaJI	CCTAGG	1 cut(s)	1778
XmiI	GTMKAC	2 cut(s)	609, 1875
XspI	CTAG	6 cut(s)	336, 890, 1275, 1350, 1779, 1917
ZrmI	AGTACT	2 cut(s)	296, 700

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.