Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4D

Orthogroup ID

OG0012427

Physical Location & Seq

Genomic Coordinates Reverse (-)

46634415 .. 46637465

Gene Structure 3051 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4DG251500.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1962 bp

ATGGCCTCAACGACCCGGCCCTTCGTCTTCGCCCTCGCATTCCTCACTATTTACACCGCCCTGCCACTCTCCCTCTCTCAGACCACCGAAGCCCCGGCGCCGGCCGCCGATTTGTGCAACGGCGTCTTCATATCCTACTCCTACACCGACGGCAAGCAGATCGCCCCCACCGCTCCGGCGCGGCAGCCCTACCGGTTCGAGTCCGTGCTGACTGTGCTCAACAACGGCGAGGAGGACCTCAAGTCGTGGAAGGTGTTCGTAGGGTTTCAGCACGGCGAGTACTTGGTCTCTGCTGCCAATGCTGTGCTCGCCGACGGGAGTGCGTATCCCGGAAGTGTGGAGAACGGCACCGTTTTCGCCGGCTTCCCGCAGACCGATCTGAAGACGGCGATCAAGACGGCGGGCGACTTGACGCAGATGCAGGTGCAGGTGAAGTTGATCGGCACTCAGTTCGGCGTGGCGCCGCCGAATGTTCCGATGCCGGCCAATATCTCTTTGGCTAATGATGGCTTTCGTTGCCCCAAGGCCTCATTGCAAGGGAAGAGTGAAATGCAAGTCTGCTGCATTGTAGACTCTACGGTGAAAACAAACATCACTGCAGATGAAGAGTTTCCCCCTCGCCAGAATGGAGATCTTTCAATCATGTATGATGTGACCCGGACGTATGGATCAAGTTACTTTGCACAAGTGACAATCTCCAACCATAACTCTCTAGGCCGTCTTGATAGTTGGAAGTTGAGCTGGGACTGGATGGCACAAGAGTTTATCAATACGATGAGAGGGGCTTATCCATCTGTTGTTGATTCTTCTGAATGCATATTTGGCGAACAAGGTCAATACTACGCGGATCTAGACTTTGCCAATGTCTTGAGTTGTGAAAAAAGGCCAACCATTATTGATCTGCCTCTGGAAAAGACTAATGACACTAAACTTGGTATGATCCCTTATTGTTGCCGAAATGGTACTATCTTGCCAACAACAATGGATGCAACCAAGTCAAAATCTTCATTCCAGATGGAAGTCTATAAAATGCCTCCAAATATCAACCGCTCTGTGATCATACCACCACAAAACTGGGCAATCAATGGCACTCTCAACCCTGATTACAAATGTGCTCCTCCTGTGCGGGTGAGTCCTAGTCAATTCCCTGATGCAAGTGGCTTACCGGTGAATTCAACTGCAGTAGCCAGCTGGCAGGTTGTGTGCAATATTACACAGGCCAAGGGAGCAAGCCCTAGATGCTGTGTTTCATTTTCTGCTTACTTCAACGAGTCTGTTGTACCATGCAAAACTTGTGCGTGTGGGTGCCCTAGAAATACAGCTCAAACTTGTAGCACAACTGCACCGGCTATGCTTCTTCCACCAGAGTCGCTTCTCGTTCCCTTTGAGAACCGAACTGTCAAGGCAAAAGCTTGGGCTGAGCTTAAACACCTAGCAGATCCGAACCCAATGCCCTGTGGTGATAACTGTGGGGTCAGCATTAACTGGCACGTACTCTCAGACTACAGTAAAGGATGGACTGCAAGGGTTACACTATTCAACTGGGGCGAAACTTCTTTTGCTGATTGGTTTACTGCAGTACAACTGGATAAAGCTGTTCCTGGTTTTGAAAAAATGTACTCTTTCAACGCTACTACTGTGGAAACGGATGGGGTCAACAATACGGTGTTTATGCAGGGTCTTGAAGGATTGAACTACCTTGTGGCAGAAGTAGATGGAGCCAACCCAAAGAAGGATCCTAGGGTGCCTGGGAAGCAGCAATCAGTGATCTCATTTACAAAGAAGCTTACTCCTGGAATTAATGTGGCTGGTAGAGATGGGTTTCCGACAAAAGTGTTCTTCAATGGGGAGGAGTGCTCACTTCCTGATATATACCCAAGCAGTGCTTACAGAACAAGCTCAGCCATGATGATGTTTCCTGTCCTCCTAATGGTTGCAGTGTTCATGTTGATGCAGCAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005886 GO:0005911 GO:0007275 GO:0008150 GO:0009506 GO:0009653 GO:0009826 GO:0009888 GO:0009932 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0021700 GO:0022622 GO:0030054 GO:0030154 GO:0031224 GO:0031225 GO:0031226 GO:0031410 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0032989 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046658 GO:0048364 GO:0048468 GO:0048469 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048856 GO:0048869 GO:0055044 GO:0060560 GO:0071695 GO:0071840 GO:0071944 GO:0090558 GO:0090627 GO:0097708 GO:0098791 GO:0099402 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

COBRA

Protein Analysis

653

Amino Acids

71.24

Weight (kDa)

5.41

Isoelectric Point (pI)

35.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
COBRA	PF04833	226 - 405	4.3e-64	COBRA-like protein
COB_C	PF25079	413 - 626	9e-101	COBRA family C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012427)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G16120
fragaria_vesca	FvH4_4g19240
malus_domestica	MD13G1159600.v1.1 MD16G1159300.v1.1
prunus_persica	Prupe.1G126300_v2.0.a1
pyrus_communis	pycom13g13600 pycom16g13430
rosa_chinensis	RchiOBHm_Chr4g0424221
rosa_laevigata	RLG00000007495
rosa_multiflora	Rmu_sc0000980.1_g000001 Rmu_sc0002260.1_g000034 Rmu_sc0019001.1_g000001
rosa_roxburghii	Rroxscaffold_5G00366170
rosa_rugosa	Rorug04G0194400
rosa_samantha	Rh4AG251800 Rh4BG257100 Rh4CG267700 Rh4DG251500
rosa_wichuraiana	Rw4G021710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	2 cut(s)	412, 418
Acc36I	ACCTGC	3 cut(s)	412, 418, 1186
AccB1I	GGYRCC	5 cut(s)	97, 347, 460, 1305, 1744
AccBSI	CCGCTC	2 cut(s)	173, 1050
AccI	GTMKAC	1 cut(s)	570
AccII	CGCG	2 cut(s)	181, 845
AclWI	GGATC	6 cut(s)	676, 855, 934, 1433, 1730, 1743
AcoI	YGGCCR	2 cut(s)	102, 483
AcsI	RAATTY	1 cut(s)	1171
AcuI	CTGAAG	1 cut(s)	401
AcyI	GRCGYC	3 cut(s)	98, 123, 461
AfaI	GTAC	6 cut(s)	281, 964, 1281, 1494, 1581, 1619
AfiI	CCNNNNNNNGG	3 cut(s)	100, 1732, 1930
AgeI	ACCGGT	2 cut(s)	192, 1165
AgsI	TTSAA	9 cut(s)	639, 1176, 1267, 1540, 1610, 1627, 1685, 1693, 1843
AhdI	GACNNNNNGTC	1 cut(s)	241
AjnI	CCWGG	3 cut(s)	1600, 1747, 1792
AjuI	GAANNNNNNNTTGG	2 cut(s)	804, 836
AluBI	AGCT	8 cut(s)	741, 1191, 1322, 1412, 1423, 1595, 1786, 1899
AluI	AGCT	8 cut(s)	741, 1191, 1322, 1412, 1423, 1595, 1786, 1899
Alw21I	GWGCWC	4 cut(s)	219, 309, 1117, 1859
Alw26I	GTCTC	1 cut(s)	292
AlwI	GGATC	6 cut(s)	676, 855, 934, 1433, 1730, 1743
AoxI	GGCC	8 cut(s)	3, 17, 102, 483, 525, 715, 884, 1218
ApeKI	GCWGC	5 cut(s)	184, 293, 561, 1756, 1954
ApoI	RAATTY	1 cut(s)	1171
AseI	ATTAAT	1 cut(s)	1800
AsiGI	ACCGGT	2 cut(s)	192, 1165
Asp700I	GAANNNNTTC	2 cut(s)	254, 609
AspA2I	CCTAGG	1 cut(s)	1739
AspLEI	GCGC	3 cut(s)	100, 181, 463
AspS9I	GGNCC	2 cut(s)	18, 235
AsuC2I	CCSGG	4 cut(s)	16, 95, 330, 658
AsuHPI	GGTGA	5 cut(s)	442, 592, 1141, 1180, 1472
AvaII	GGWCC	1 cut(s)	235
AvrII	CCTAGG	1 cut(s)	1739
BaeGI	GKGCMC	1 cut(s)	1310
BamHI	GGATCC	1 cut(s)	1735
BanI	GGYRCC	5 cut(s)	97, 347, 460, 1305, 1744
BbsI	GAAGAC	3 cut(s)	19, 118, 389
Bbv12I	GWGCWC	4 cut(s)	219, 309, 1117, 1859
BbvI	GCAGC	4 cut(s)	196, 280, 548, 1768
BccI	CCATC	8 cut(s)	500, 745, 799, 1009, 1509, 1643, 1709, 1811
BceAI	ACGGC	8 cut(s)	136, 166, 241, 289, 361, 402, 414, 702
BciT130I	CCWGG	3 cut(s)	1602, 1749, 1794
BciVI	GTATCC	1 cut(s)	336
BclI	TGATCA	1 cut(s)	1056
BcnI	CCSGG	4 cut(s)	16, 95, 330, 658
BcoDI	GTCTC	1 cut(s)	292
BfaI	CTAG	7 cut(s)	713, 851, 1137, 1236, 1311, 1433, 1740
BfmI	CTRYAG	4 cut(s)	597, 1179, 1504, 1575
BfoI	RGCGCY	2 cut(s)	101, 464
BfuAI	ACCTGC	3 cut(s)	412, 418, 1186
BfuI	GTATCC	1 cut(s)	336
BglII	AGATCT	1 cut(s)	631
BisI	GCNGC	8 cut(s)	105, 182, 185, 294, 464, 562, 1757, 1955
BlnI	CCTAGG	1 cut(s)	1739
BlpI	GCTNAGC	2 cut(s)	1419, 1900
BlsI	GCNGC	8 cut(s)	106, 183, 186, 295, 465, 563, 1758, 1956
BmcAI	AGTACT	1 cut(s)	281
Bme1390I	CCNGG	7 cut(s)	16, 95, 330, 658, 1602, 1749, 1794
Bme18I	GGWCC	1 cut(s)	235
BmeRI	GACNNNNNGTC	1 cut(s)	241
BmgT120I	GGNCC	2 cut(s)	18, 235
BmiI	GGNNCC	7 cut(s)	99, 349, 462, 1307, 1720, 1737, 1746
BmrFI	CCNGG	7 cut(s)	16, 95, 330, 658, 1602, 1749, 1794
BmrI	ACTGGG	2 cut(s)	1084, 1552
BmsI	GCATC	6 cut(s)	408, 468, 976, 1141, 1229, 1941
BmuI	ACTGGG	2 cut(s)	1084, 1552
BpiI	GAAGAC	3 cut(s)	19, 118, 389
BplI	GAGNNNNNCTC	2 cut(s)	1841, 1873
Bpu1102I	GCTNAGC	2 cut(s)	1419, 1900
BpuEI	CTTGAG	2 cut(s)	224, 889
BpuMI	CCSGG	4 cut(s)	16, 95, 330, 658
BsaAI	YACGTR	1 cut(s)	1492
BsaHI	GRCGYC	3 cut(s)	98, 123, 461
BsaI	GGTCTC	1 cut(s)	292
BsaJI	CCNNGG	5 cut(s)	93, 522, 1221, 1739, 1748
BsaWI	WCCGGW	2 cut(s)	192, 1165
Bsc4I	CCNNNNNNNGG	3 cut(s)	100, 1732, 1930
Bse118I	RCCGGY	6 cut(s)	100, 192, 359, 481, 1165, 1345
Bse1I	ACTGG	5 cut(s)	752, 1079, 1490, 1547, 1590
Bse3DI	GCAATG	1 cut(s)	530
BseBI	CCWGG	3 cut(s)	1602, 1749, 1794
BseDI	CCNNGG	5 cut(s)	93, 522, 1221, 1739, 1748
BseGI	GGATG	4 cut(s)	756, 991, 1520, 1654
BseLI	CCNNNNNNNGG	3 cut(s)	100, 1732, 1930
BseMI	GCAATG	1 cut(s)	530
BseMII	CTCAG	5 cut(s)	92, 461, 1410, 1512, 1914
BseNI	ACTGG	5 cut(s)	752, 1079, 1490, 1547, 1590
BseRI	GAGGAG	3 cut(s)	245, 1107, 1865
BseSI	GKGCMC	1 cut(s)	1310
BseX3I	CGGCCG	1 cut(s)	102
BseXI	GCAGC	4 cut(s)	196, 280, 548, 1768
BseYI	CCCAGC	1 cut(s)	741
BsgI	GTGCAG	2 cut(s)	446, 1326
Bsh1236I	CGCG	2 cut(s)	181, 845
Bsh1285I	CGRYCG	1 cut(s)	105
BshFI	GGCC	8 cut(s)	5, 19, 104, 485, 527, 717, 886, 1220
BshNI	GGYRCC	5 cut(s)	97, 347, 460, 1305, 1744
BshTI	ACCGGT	2 cut(s)	192, 1165
BsiEI	CGRYCG	1 cut(s)	105
BsiHKAI	GWGCWC	4 cut(s)	219, 309, 1117, 1859
BslFI	GGGAC	1 cut(s)	758
BslI	CCNNNNNNNGG	3 cut(s)	100, 1732, 1930
BsmAI	GTCTC	1 cut(s)	292
BsmFI	GGGAC	1 cut(s)	758
BsmI	GAATGC	2 cut(s)	38, 818
BsnI	GGCC	8 cut(s)	5, 19, 104, 485, 527, 717, 886, 1220
Bso31I	GGTCTC	1 cut(s)	292
Bsp1286I	GDGCHC	5 cut(s)	219, 309, 1117, 1310, 1859
Bsp1720I	GCTNAGC	2 cut(s)	1419, 1900
BspANI	GGCC	8 cut(s)	5, 19, 104, 485, 527, 717, 886, 1220
BspCNI	CTCAG	5 cut(s)	91, 460, 1411, 1511, 1913
BspFNI	CGCG	2 cut(s)	181, 845
BspLI	GGNNCC	7 cut(s)	99, 349, 462, 1307, 1720, 1737, 1746
BspMAI	CTGCAG	3 cut(s)	601, 1183, 1579
BspMI	ACCTGC	3 cut(s)	412, 418, 1186
BspPI	GGATC	6 cut(s)	676, 855, 934, 1433, 1730, 1743
BspT107I	GGYRCC	5 cut(s)	97, 347, 460, 1305, 1744
BspTNI	GGTCTC	1 cut(s)	292
BsrBI	CCGCTC	2 cut(s)	173, 1050
BsrDI	GCAATG	1 cut(s)	530
BsrFI	RCCGGY	6 cut(s)	100, 192, 359, 481, 1165, 1345
BsrI	ACTGG	5 cut(s)	752, 1079, 1490, 1547, 1590
BssAI	RCCGGY	6 cut(s)	100, 192, 359, 481, 1165, 1345
BssECI	CCNNGG	5 cut(s)	93, 522, 1221, 1739, 1748
BssNI	GRCGYC	3 cut(s)	98, 123, 461
BssT1I	CCWWGG	3 cut(s)	522, 1221, 1739
Bst2UI	CCWGG	3 cut(s)	1602, 1749, 1794
Bst4CI	ACNGT	8 cut(s)	214, 352, 580, 1399, 1469, 1508, 1639, 1666
Bst6I	CTCTTC	2 cut(s)	536, 600
BstACI	GRCGYC	3 cut(s)	98, 123, 461
BstBAI	YACGTR	1 cut(s)	1492
BstC8I	GCNNGC	9 cut(s)	102, 155, 309, 361, 403, 483, 1189, 1193, 1231
BstDEI	CTNAG	5 cut(s)	78, 447, 1419, 1498, 1900
BstF5I	GGATG	4 cut(s)	756, 991, 1520, 1654
BstFNI	CGCG	2 cut(s)	181, 845
BstH2I	RGCGCY	2 cut(s)	101, 464
BstHHI	GCGC	3 cut(s)	100, 181, 463
BstMAI	GTCTC	1 cut(s)	292
BstMCI	CGRYCG	1 cut(s)	105
BstNI	CCWGG	3 cut(s)	1602, 1749, 1794
BstSCI	CCNGG	7 cut(s)	14, 93, 328, 656, 1600, 1747, 1792
BstSFI	CTRYAG	4 cut(s)	597, 1179, 1504, 1575
BstSLI	GKGCMC	1 cut(s)	1310
BstUI	CGCG	2 cut(s)	181, 845
BstV1I	GCAGC	4 cut(s)	196, 280, 548, 1768
BstV2I	GAAGAC	3 cut(s)	19, 118, 389
BstX2I	RGATCY	4 cut(s)	631, 847, 1438, 1735
BstXI	CCANNNNNNTGG	1 cut(s)	1074
BstYI	RGATCY	4 cut(s)	631, 847, 1438, 1735
BstZI	CGGCCG	1 cut(s)	102
BsuI	GTATCC	1 cut(s)	336
BsuRI	GGCC	8 cut(s)	5, 19, 104, 485, 527, 717, 886, 1220
BtsCI	GGATG	4 cut(s)	756, 991, 1520, 1654
BtsI	GCAGTG	3 cut(s)	594, 1888, 1944
BtsIMutI	CAGTG	4 cut(s)	594, 1770, 1888, 1944
BveI	ACCTGC	3 cut(s)	412, 418, 1186
Cac8I	GCNNGC	9 cut(s)	102, 155, 309, 361, 403, 483, 1189, 1193, 1231
CfoI	GCGC	3 cut(s)	100, 181, 463
Cfr10I	RCCGGY	6 cut(s)	100, 192, 359, 481, 1165, 1345
Cfr13I	GGNCC	2 cut(s)	18, 235
CseI	GACGC	2 cut(s)	112, 421
Csp6I	GTAC	6 cut(s)	280, 963, 1280, 1493, 1580, 1618
CspAI	ACCGGT	2 cut(s)	192, 1165
CviAII	CATG	4 cut(s)	643, 1284, 1906, 1945
CviQI	GTAC	6 cut(s)	280, 963, 1280, 1493, 1580, 1618
DdeI	CTNAG	5 cut(s)	78, 447, 1419, 1498, 1900
DinI	GGCGCC	2 cut(s)	99, 462
DriI	GACNNNNNGTC	1 cut(s)	241
EaeI	YGGCCR	2 cut(s)	102, 483
EagI	CGGCCG	1 cut(s)	102
Eam1104I	CTCTTC	2 cut(s)	536, 600
Eam1105I	GACNNNNNGTC	1 cut(s)	241
EarI	CTCTTC	2 cut(s)	536, 600
EclXI	CGGCCG	1 cut(s)	102
Eco130I	CCWWGG	3 cut(s)	522, 1221, 1739
Eco147I	AGGCCT	1 cut(s)	527
Eco31I	GGTCTC	1 cut(s)	292
Eco47I	GGWCC	1 cut(s)	235
Eco52I	CGGCCG	1 cut(s)	102
Eco57I	CTGAAG	1 cut(s)	401
EcoO109I	RGGNCCY	1 cut(s)	235
EcoRI	GAATTC	1 cut(s)	1171
EcoRII	CCWGG	3 cut(s)	1600, 1747, 1792
EcoT14I	CCWWGG	3 cut(s)	522, 1221, 1739
EcoT22I	ATGCAT	1 cut(s)	818
EgeI	GGCGCC	2 cut(s)	99, 462
EheI	GGCGCC	2 cut(s)	99, 462
ErhI	CCWWGG	3 cut(s)	522, 1221, 1739
FaeI	CATG	4 cut(s)	646, 1287, 1909, 1948
FalI	AAGNNNNNCTT	2 cut(s)	1870, 1902
FaqI	GGGAC	1 cut(s)	758
FatI	CATG	4 cut(s)	642, 1283, 1905, 1944
FauI	CCCGC	3 cut(s)	375, 394, 1119
FbaI	TGATCA	1 cut(s)	1056
FblI	GTMKAC	1 cut(s)	570
Fnu4HI	GCNGC	8 cut(s)	105, 182, 185, 294, 464, 562, 1757, 1955
FokI	GGATG	4 cut(s)	763, 998, 1527, 1661
Fsp4HI	GCNGC	8 cut(s)	105, 182, 185, 294, 464, 562, 1757, 1955
FspBI	CTAG	7 cut(s)	713, 851, 1137, 1236, 1311, 1433, 1740
GlaI	GCGC	3 cut(s)	99, 180, 462
GluI	GCNGC	8 cut(s)	105, 182, 185, 294, 464, 562, 1757, 1955
GsaI	CCCAGC	1 cut(s)	745
HaeII	RGCGCY	2 cut(s)	101, 464
HaeIII	GGCC	8 cut(s)	5, 19, 104, 485, 527, 717, 886, 1220
HgaI	GACGC	2 cut(s)	112, 421
HhaI	GCGC	3 cut(s)	100, 181, 463
Hin1I	GRCGYC	3 cut(s)	98, 123, 461
Hin1II	CATG	4 cut(s)	646, 1287, 1909, 1948
Hin6I	GCGC	3 cut(s)	98, 179, 461
HinP1I	GCGC	3 cut(s)	98, 179, 461
HincII	GTYRAC	1 cut(s)	1657
HindII	GTYRAC	1 cut(s)	1657
HindIII	AAGCTT	2 cut(s)	1410, 1784
HinfI	GANTC	6 cut(s)	200, 572, 803, 1132, 1271, 1367
HphI	GGTGA	5 cut(s)	442, 592, 1141, 1180, 1472
Hpy166II	GTNNAC	3 cut(s)	571, 1572, 1657
Hpy188I	TCNGA	7 cut(s)	81, 381, 477, 811, 1443, 1501, 1827
Hpy188III	TCNNGA	8 cut(s)	394, 722, 851, 868, 908, 1012, 1682, 1865
Hpy8I	GTNNAC	3 cut(s)	571, 1572, 1657
Hpy99I	CGWCG	2 cut(s)	152, 317
HpyAV	CCTTC	4 cut(s)	31, 244, 1679, 1726
HpyCH4III	ACNGT	8 cut(s)	214, 352, 580, 1399, 1469, 1508, 1639, 1666
HpyCH4IV	ACGT	2 cut(s)	662, 1491
HpyF3I	CTNAG	5 cut(s)	78, 447, 1419, 1498, 1900
HpySE526I	ACGT	2 cut(s)	662, 1491
Hsp92I	GRCGYC	3 cut(s)	98, 123, 461
Hsp92II	CATG	4 cut(s)	646, 1287, 1909, 1948
HspAI	GCGC	3 cut(s)	98, 179, 461
KasI	GGCGCC	2 cut(s)	97, 460
KroI	GCCGGC	3 cut(s)	100, 359, 481
KroNI	GCCGGC	3 cut(s)	102, 361, 483
Ksp22I	TGATCA	1 cut(s)	1056
LmnI	GCTCC	4 cut(s)	178, 1120, 1226, 1718
Lsp1109I	GCAGC	4 cut(s)	196, 280, 548, 1768
LweI	GCATC	6 cut(s)	408, 468, 976, 1141, 1229, 1941
MaeI	CTAG	7 cut(s)	713, 851, 1137, 1236, 1311, 1433, 1740
MaeII	ACGT	2 cut(s)	662, 1491
MaeIII	GTNAC	4 cut(s)	652, 674, 688, 1528
MbiI	CCGCTC	2 cut(s)	173, 1050
MboII	GAAGA	9 cut(s)	19, 118, 394, 553, 617, 798, 996, 1349, 1831
MflI	RGATCY	4 cut(s)	631, 847, 1438, 1735
MhlI	GDGCHC	5 cut(s)	219, 309, 1117, 1310, 1859
MluCI	AATT	3 cut(s)	1142, 1171, 1797
Mly113I	GGCGCC	2 cut(s)	98, 461
MlyI	GAGTC	5 cut(s)	209, 566, 1141, 1280, 1376
MmeI	TCCRAC	3 cut(s)	710, 723, 1850
Mph1103I	ATGCAT	1 cut(s)	818
MroNI	GCCGGC	3 cut(s)	100, 359, 481
MroXI	GAANNNNTTC	2 cut(s)	254, 609
MseI	TTAA	3 cut(s)	1425, 1482, 1800
MslI	CAYNNNNRTG	2 cut(s)	1910, 1949
MspA1I	CMGCKG	1 cut(s)	1191
MspR9I	CCNGG	7 cut(s)	16, 95, 330, 658, 1602, 1749, 1794
Mva1269I	GAATGC	2 cut(s)	38, 818
MvaI	CCWGG	3 cut(s)	1602, 1749, 1794
MvnI	CGCG	2 cut(s)	181, 845
NaeI	GCCGGC	3 cut(s)	102, 361, 483
NarI	GGCGCC	2 cut(s)	98, 461
NciI	CCSGG	4 cut(s)	16, 95, 330, 658
NgoMIV	GCCGGC	3 cut(s)	100, 359, 481
NlaIII	CATG	4 cut(s)	646, 1287, 1909, 1948
NlaIV	GGNNCC	7 cut(s)	99, 349, 462, 1307, 1720, 1737, 1746
NmuCI	GTSAC	2 cut(s)	652, 688
NsiI	ATGCAT	1 cut(s)	818
PaqCI	CACCTGC	2 cut(s)	412, 418
PceI	AGGCCT	1 cut(s)	527
PctI	GAATGC	2 cut(s)	38, 818
PdiI	GCCGGC	3 cut(s)	102, 361, 483
PdmI	GAANNNNTTC	2 cut(s)	254, 609
PfeI	GAWTC	1 cut(s)	803
PfoI	TCCNGGA	2 cut(s)	328, 1792
PinAI	ACCGGT	2 cut(s)	192, 1165
PkrI	GCNGC	8 cut(s)	106, 183, 186, 295, 465, 563, 1758, 1956
PleI	GAGTC	5 cut(s)	208, 566, 1140, 1279, 1375
PluTI	GGCGCC	2 cut(s)	101, 464
PpsI	GAGTC	5 cut(s)	208, 566, 1140, 1279, 1375
Ppu21I	YACGTR	1 cut(s)	1492
PpuMI	RGGWCCY	1 cut(s)	235
PshBI	ATTAAT	1 cut(s)	1800
Psp5II	RGGWCCY	1 cut(s)	235
Psp6I	CCWGG	3 cut(s)	1600, 1747, 1792
PspFI	CCCAGC	1 cut(s)	741
PspGI	CCWGG	3 cut(s)	1600, 1747, 1792
PspN4I	GGNNCC	7 cut(s)	99, 349, 462, 1307, 1720, 1737, 1746
PspPI	GGNCC	2 cut(s)	18, 235
PspPPI	RGGWCCY	1 cut(s)	235
PstI	CTGCAG	3 cut(s)	601, 1183, 1579
PsuI	RGATCY	4 cut(s)	631, 847, 1438, 1735
PvuII	CAGCTG	1 cut(s)	1191
RsaI	GTAC	6 cut(s)	281, 964, 1281, 1494, 1581, 1619
RsaNI	GTAC	6 cut(s)	280, 963, 1280, 1493, 1580, 1618
RseI	CAYNNNNRTG	2 cut(s)	1910, 1949
SaqAI	TTAA	3 cut(s)	1425, 1482, 1800
SatI	GCNGC	8 cut(s)	105, 182, 185, 294, 464, 562, 1757, 1955
Sau96I	GGNCC	2 cut(s)	18, 235
ScaI	AGTACT	1 cut(s)	281
SchI	GAGTC	5 cut(s)	209, 566, 1141, 1280, 1376
ScrFI	CCNGG	7 cut(s)	16, 95, 330, 658, 1602, 1749, 1794
SduI	GDGCHC	5 cut(s)	219, 309, 1117, 1310, 1859
SfaNI	GCATC	6 cut(s)	408, 468, 976, 1141, 1229, 1941
SfcI	CTRYAG	4 cut(s)	597, 1179, 1504, 1575
SfoI	GGCGCC	2 cut(s)	99, 462
SinI	GGWCC	1 cut(s)	235
SmiMI	CAYNNNNRTG	2 cut(s)	1910, 1949
SmlI	CTYRAG	2 cut(s)	239, 868
SmoI	CTYRAG	2 cut(s)	239, 868
Sse9I	AATT	3 cut(s)	1142, 1171, 1797
SseBI	AGGCCT	1 cut(s)	527
SspDI	GGCGCC	2 cut(s)	97, 460
SspI	AATATT	1 cut(s)	1210
SspMI	CTAG	7 cut(s)	713, 851, 1137, 1236, 1311, 1433, 1740
StuI	AGGCCT	1 cut(s)	527
StyD4I	CCNGG	7 cut(s)	14, 93, 328, 656, 1600, 1747, 1792
StyI	CCWWGG	3 cut(s)	522, 1221, 1739
TaaI	ACNGT	8 cut(s)	214, 352, 580, 1399, 1469, 1508, 1639, 1666
TaiI	ACGT	2 cut(s)	665, 1494
TaqI	TCGA	1 cut(s)	198
TaqII	GACCGA	1 cut(s)	389
TasI	AATT	3 cut(s)	1142, 1171, 1797
TatI	WGTACW	3 cut(s)	279, 1579, 1617
TauI	GCSGC	3 cut(s)	107, 184, 466
TfiI	GAWTC	1 cut(s)	803
Tru1I	TTAA	3 cut(s)	1425, 1482, 1800
Tru9I	TTAA	3 cut(s)	1425, 1482, 1800
TscAI	CASTG	4 cut(s)	601, 1770, 1888, 1944
TseFI	GTSAC	2 cut(s)	652, 688
TseI	GCWGC	5 cut(s)	184, 293, 561, 1756, 1954
Tsp45I	GTSAC	2 cut(s)	652, 688
TspDTI	ATGAA	5 cut(s)	118, 618, 996, 1239, 1933
TspGWI	ACGGA	2 cut(s)	193, 1661
TspRI	CASTG	4 cut(s)	601, 1770, 1888, 1944
VpaK11BI	GGWCC	1 cut(s)	235
VspI	ATTAAT	1 cut(s)	1800
XapI	RAATTY	1 cut(s)	1171
XbaI	TCTAGA	1 cut(s)	850
XcmI	CCANNNNNNNNNTGG	2 cut(s)	493, 1071
XmaJI	CCTAGG	1 cut(s)	1739
XmiI	GTMKAC	1 cut(s)	570
XmnI	GAANNNNTTC	2 cut(s)	254, 609
XspI	CTAG	7 cut(s)	713, 851, 1137, 1236, 1311, 1433, 1740
ZrmI	AGTACT	1 cut(s)	281
Zsp2I	ATGCAT	1 cut(s)	818

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.