Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr16

Orthogroup ID

OG0012427

Physical Location & Seq

Genomic Coordinates Forward (+)

9612924 .. 9615950

Gene Structure 3027 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom16g13430.3

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2244 bp

ATGGATTCAAAATCAAATAGGAAGAGAGGGGAAAGGTTCGTTGAGACAAAGACAAAAGGACCTTTTCACGCTCAAAACAAACACAAGCATTATCCAACGTTACTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTACGCGTTTGAAAAAAAGCGCACACAGAAACAGTCGCACATATATAAACCCTATCTGTTTCTCCAAATTCAAAAATCCAAACTTTGGAGAACCCCAAGAAAACCAAAAGCCATGGACTCGGGCTCCCGGAACTTGCCTCCGATCATGGCCCTCAATCTTCTCATTATCCTCGTTATCCTGCCCTTCTCACTCGCCCAGCCAACCGCCGCAGCTCCGGCGCCGGCCTCCGACTCCTGCAACGGCGTCTTCCTGTCCTACGCCTATACTACAGGAGCCCAGCTCCCGCCCGAGGTCAAAGACCCGAAACAGCAGCCTTACAAGTTTGAGTCGGTTCTCACGGTGCTCAACAACGGGTTCGACGATCTCAAGTCGTGGAGGGTGTTTGTGGGGTTTACCAACAACGAGTACTTGGTCTCTGCCTCCAATGCCGTTTTGGCCGACGGTACTAGTATCCCTGGGGGTGTCGGAAACGGCACCGTTTTTGCTGGGTATCCGATGACCGATCTGAAGACGGCGGTTAAGACCGCGGGGGACTTGGCCCAGATGCAGGTTCAGGTCAAATTGATCGGGACCCAGTTCGGTGTGGCCCCACCCAAAGTTCCCATGCCTTCCAATATTACTCTGGCCAATGATGGGTTCGTCTGCCCAGCGGCTGCAATGCAAGGGACCCATGAAATGCAAGTCTGTTGCACCGTAGACGCAAATTTTAAAACGAACATCACTGTGGATGAAGAGTTCCTCCCCCGTCAAAAGGGAGATCTTACAATTATGTATGATTTGATCAGTACTCGGGCAACAGATTACACGGCGCAAGTGACAATCGCCAACCATAACTCCCTTGGTCGTCTTGATAATTGGAAACTGAGCTGGGACTGGATGGAAGATGAGTTCATATTTTCAACGAAAGGGGCTTATCCATCTGTTGTTGATTCTTCTGATTGTATATTCGGTAAACAAGGTACATTCTACGAGAATCTAGACTTCTCAACTGTATTGAATTGTGAAAGACGGCCAACAATTATTGACCTTCCTCCAACAAAAGCCAATGAGACACTTCTTGGTGTGGTCTCTTATTGTTGCCGAAATGGTACGATCTTGCCACCAACAATGGATGCAAGCAAGTCAGTTTCATCATTCCAGATGCAAGTTTTTAAAATGCCTCCAAATCTCAACCGGTCCCAGCTCTCACCTCCACAAAACTGGGGAATCAAAGGCACGCTCAACCCTGATTATAAATGTGGCCCTCCTGTCCGGGTGAGTCCCAGTCAGTTCCCTGACCGATCTGGCTTGCCAGTGAATATATCTGCAGTAGCCAGCTGGCAGGTTGTGTGCAATATTACCCAACCCAAAGGAGCAATCCCTAGTTGCTGTGTTTCATTTTCTGCTTTCTACAATGAGTCTGTCGTCCCATGCAACACTTGCGCTTGTGGCTGCCCTAGTAATACAGCTCGAACTTGTAGCACAACTGCACCAGCTATGCTTCTCCCACCAAAGTCTCTTCTTGTTCCCTTTGACAACCGAACTGTCAAGGCAAAATCTTGGGCTGATATTAAACATCTACCATTCCCAAACCCGACACCTTGTGGTGATAACTGTGGGATCAGCATCAACTGGCATTTATATACAGACTACTCTCGTGGATGGAGTGCAAGAGTCACAGTCTTCAATTGGGATGAAACGGCCTTTGTTGATTGGTTCGCTGCAGTACAACTGGATAAAGCAGGCCCTGGTTTCGAAAAGGCGTACTCTTTTAACGCAAGTCACTTGGAAATTAATGGTGTCAATAATACCGTATTCATGCAAGGCCCCAAAGGATTGAACTATCTTGTGGCGGAAACAGATGGAGCCAACCCAAAGAAGGATCCTAGGGTGCCTGGGAAACAGCAGTCAGTGCTCTCGTTTACAAAGAAGATTACTCCCGGAATCAATTTGGTTGGTGGAGATGGGTTTCCGACGAAAGTATACTTTAACGGGGAGGAGTGCTCTCTTCCTACAGCATATCCTAGCAGTGGTTACAGAAAAATCACATCTCTAATTTTCTCGGTTCTCGTAATGGTTGTAGCGTTTATGGTGATGCAGCAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000902 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005794 GO:0005802 GO:0005886 GO:0005911 GO:0007275 GO:0008150 GO:0009506 GO:0009653 GO:0009826 GO:0009888 GO:0009932 GO:0009987 GO:0010015 GO:0010053 GO:0010054 GO:0012505 GO:0016020 GO:0016043 GO:0016049 GO:0021700 GO:0022622 GO:0030054 GO:0030154 GO:0031224 GO:0031225 GO:0031226 GO:0031410 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0032989 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044444 GO:0044446 GO:0044459 GO:0044464 GO:0046658 GO:0048364 GO:0048468 GO:0048469 GO:0048589 GO:0048731 GO:0048764 GO:0048765 GO:0048856 GO:0048869 GO:0055044 GO:0060560 GO:0071695 GO:0071840 GO:0071944 GO:0090558 GO:0090627 GO:0097708 GO:0098791 GO:0099402 GO:1905392

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

COBRA

Protein Analysis

748

Amino Acids

81.9

Weight (kDa)

8.85

Isoelectric Point (pI)

36.96

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012427)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G16120
fragaria_vesca	FvH4_4g19240
malus_domestica	MD13G1159600.v1.1 MD16G1159300.v1.1
prunus_persica	Prupe.1G126300_v2.0.a1
pyrus_communis	pycom13g13600 pycom16g13430
rosa_chinensis	RchiOBHm_Chr4g0424221
rosa_laevigata	RLG00000007495
rosa_multiflora	Rmu_sc0000980.1_g000001 Rmu_sc0002260.1_g000034 Rmu_sc0019001.1_g000001
rosa_roxburghii	Rroxscaffold_5G00366170
rosa_rugosa	Rorug04G0194400
rosa_samantha	Rh4AG251800 Rh4BG257100 Rh4CG267700 Rh4DG251500
rosa_wichuraiana	Rw4G021710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1392
Acc36I	ACCTGC	2 cut(s)	697, 1471
AccB1I	GGYRCC	3 cut(s)	376, 632, 2029
AccB7I	CCANNNNNTGG	1 cut(s)	243
AccI	GTMKAC	2 cut(s)	855, 2121
AccII	CGCG	2 cut(s)	164, 686
AciI	CCGC	8 cut(s)	363, 366, 443, 674, 684, 686, 809, 1991
AclI	AACGTT	1 cut(s)	98
AclWI	GGATC	3 cut(s)	1766, 2015, 2028
AcoI	YGGCCR	3 cut(s)	594, 783, 1169
AcsI	RAATTY	2 cut(s)	225, 862
AcuI	CTGAAG	1 cut(s)	686
AcyI	GRCGYC	2 cut(s)	377, 402
AdeI	CACNNNGTG	1 cut(s)	1742
AfaI	GTAC	7 cut(s)	566, 604, 946, 1120, 1249, 1866, 1904
AfiI	CCNNNNNNNGG	7 cut(s)	243, 448, 706, 792, 910, 2017, 2168
AflIII	ACRYGT	1 cut(s)	162
AgeI	ACCGGT	1 cut(s)	1332
AgsI	TTSAA	7 cut(s)	9, 170, 230, 1059, 1156, 1825, 1978
AhlI	ACTAGT	1 cut(s)	605
AjnI	CCWGG	3 cut(s)	613, 1885, 2032
AjuI	GAANNNNNNNTTGG	2 cut(s)	779, 811
AloI	GAACNNNNNNTCC	4 cut(s)	878, 910, 1031, 1063
AluBI	AGCT	7 cut(s)	371, 439, 1026, 1342, 1476, 1607, 1634
AluI	AGCT	7 cut(s)	371, 439, 1026, 1342, 1476, 1607, 1634
Alw21I	GWGCWC	3 cut(s)	504, 2055, 2144
Alw26I	GTCTC	5 cut(s)	38, 577, 1202, 1231, 1659
AlwI	GGATC	3 cut(s)	1766, 2015, 2028
AlwNI	CAGNNNCTG	2 cut(s)	812, 1886
Ama87I	CYCGRG	3 cut(s)	277, 446, 948
ApeKI	GCWGC	6 cut(s)	368, 469, 812, 1590, 1859, 2236
ApoI	RAATTY	2 cut(s)	225, 862
AseI	ATTAAT	1 cut(s)	1932
AsiGI	ACCGGT	1 cut(s)	1332
AspA2I	CCTAGG	1 cut(s)	2024
AspLEI	GCGC	4 cut(s)	180, 379, 970, 1583
AsuC2I	CCSGG	3 cut(s)	286, 1412, 2079
AsuHPI	GGTGA	4 cut(s)	1338, 1426, 1757, 2242
AsuII	TTCGAA	1 cut(s)	1893
AvaI	CYCGRG	3 cut(s)	277, 446, 948
AvaII	GGWCC	4 cut(s)	59, 729, 825, 1335
AvrII	CCTAGG	1 cut(s)	2024
BalI	TGGCCA	1 cut(s)	785
BamHI	GGATCC	1 cut(s)	2020
BanI	GGYRCC	3 cut(s)	376, 632, 2029
BanII	GRGCYC	2 cut(s)	284, 436
BauI	CACGAG	1 cut(s)	1794
BbsI	GAAGAC	3 cut(s)	397, 674, 1813
Bbv12I	GWGCWC	3 cut(s)	504, 2055, 2144
BbvI	GCAGC	5 cut(s)	380, 481, 799, 1577, 1846
BccI	CCATC	6 cut(s)	785, 1030, 1084, 1794, 1994, 2096
BceAI	ACGGC	7 cut(s)	415, 572, 646, 687, 981, 1184, 1854
BciT130I	CCWGG	3 cut(s)	615, 1887, 2034
BciVI	GTATCC	2 cut(s)	620, 660
BclI	TGATCA	1 cut(s)	939
BcnI	CCSGG	3 cut(s)	286, 1412, 2079
BcoDI	GTCTC	5 cut(s)	38, 577, 1202, 1231, 1659
BcuI	ACTAGT	1 cut(s)	605
BfaI	CTAG	6 cut(s)	606, 1136, 1521, 1596, 2025, 2163
BfmI	CTRYAG	4 cut(s)	426, 1464, 1860, 2151
BfoI	RGCGCY	1 cut(s)	380
BfuAI	ACCTGC	2 cut(s)	697, 1471
BfuI	GTATCC	2 cut(s)	620, 660
BglI	GCCNNNNNGGC	1 cut(s)	593
BglII	AGATCT	1 cut(s)	916
BisI	GCNGC	8 cut(s)	366, 369, 470, 810, 813, 1591, 1860, 2237
BlnI	CCTAGG	1 cut(s)	2024
BlsI	GCNGC	8 cut(s)	367, 370, 471, 811, 814, 1592, 1861, 2238
BmcAI	AGTACT	2 cut(s)	566, 946
Bme1390I	CCNGG	6 cut(s)	286, 615, 1412, 1887, 2034, 2079
Bme18I	GGWCC	4 cut(s)	59, 729, 825, 1335
BmeT110I	CYCGRG	3 cut(s)	277, 446, 948
BmrFI	CCNGG	6 cut(s)	286, 615, 1412, 1887, 2034, 2079
BmrI	ACTGGG	3 cut(s)	727, 1369, 1416
BmsI	GCATC	5 cut(s)	693, 1261, 1290, 1773, 2223
BmuI	ACTGGG	3 cut(s)	727, 1369, 1416
BpiI	GAAGAC	3 cut(s)	397, 674, 1813
BplI	GAGNNNNNCTC	4 cut(s)	423, 455, 2126, 2158
Bpu14I	TTCGAA	1 cut(s)	1893
BpuEI	CTTGAG	1 cut(s)	509
BpuMI	CCSGG	3 cut(s)	286, 1412, 2079
BsaBI	GATNNNNATC	1 cut(s)	1763
BsaHI	GRCGYC	2 cut(s)	377, 402
BsaI	GGTCTC	2 cut(s)	577, 1231
BsaJI	CCNNGG	9 cut(s)	270, 447, 613, 614, 684, 997, 1885, 2024, 2033
BsaWI	WCCGGW	1 cut(s)	1332
BsaXI	ACNNNNNCTCC	4 cut(s)	266, 296, 977, 1007
Bsc4I	CCNNNNNNNGG	7 cut(s)	243, 448, 706, 792, 910, 2017, 2168
Bse118I	RCCGGY	2 cut(s)	379, 1332
Bse1I	ACTGG	7 cut(s)	733, 1037, 1364, 1422, 1451, 1775, 1875
Bse3DI	GCAATG	1 cut(s)	822
Bse8I	GATNNNNATC	1 cut(s)	1763
BseBI	CCWGG	3 cut(s)	615, 1887, 2034
BseDI	CCNNGG	9 cut(s)	270, 447, 613, 614, 684, 997, 1885, 2024, 2033
BseGI	GGATG	5 cut(s)	892, 1041, 1276, 1805, 1837
BseJI	GATNNNNATC	1 cut(s)	1763
BseLI	CCNNNNNNNGG	7 cut(s)	243, 448, 706, 792, 910, 2017, 2168
BseMI	GCAATG	1 cut(s)	822
BseMII	CTCAG	1 cut(s)	1013
BseNI	ACTGG	7 cut(s)	733, 1037, 1364, 1422, 1451, 1775, 1875
BseRI	GAGGAG	1 cut(s)	2150
BseXI	GCAGC	5 cut(s)	380, 481, 799, 1577, 1846
BseYI	CCCAGC	6 cut(s)	354, 435, 644, 805, 1026, 1338
BsgI	GTGCAG	1 cut(s)	1611
Bsh1236I	CGCG	2 cut(s)	164, 686
BshNI	GGYRCC	3 cut(s)	376, 632, 2029
BshTI	ACCGGT	1 cut(s)	1332
BsiHKAI	GWGCWC	3 cut(s)	504, 2055, 2144
BsiHKCI	CYCGRG	3 cut(s)	277, 446, 948
BsiSI	CCGG	6 cut(s)	286, 374, 380, 1333, 1411, 2079
BslFI	GGGAC	7 cut(s)	704, 742, 838, 1043, 1321, 1404, 1550
BslI	CCNNNNNNNGG	7 cut(s)	243, 448, 706, 792, 910, 2017, 2168
BsmAI	GTCTC	5 cut(s)	38, 577, 1202, 1231, 1659
BsmFI	GGGAC	7 cut(s)	704, 742, 838, 1043, 1321, 1404, 1550
Bso31I	GGTCTC	2 cut(s)	577, 1231
BsoBI	CYCGRG	3 cut(s)	277, 446, 948
Bsp119I	TTCGAA	1 cut(s)	1893
Bsp1286I	GDGCHC	5 cut(s)	284, 436, 504, 2055, 2144
Bsp19I	CCATGG	1 cut(s)	270
BspACI	CCGC	8 cut(s)	363, 366, 443, 674, 684, 686, 809, 1991
BspCNI	CTCAG	1 cut(s)	1014
BspFNI	CGCG	2 cut(s)	164, 686
BspMAI	CTGCAG	2 cut(s)	1468, 1864
BspMI	ACCTGC	2 cut(s)	697, 1471
BspPI	GGATC	3 cut(s)	1766, 2015, 2028
BspT104I	TTCGAA	1 cut(s)	1893
BspT107I	GGYRCC	3 cut(s)	376, 632, 2029
BspTNI	GGTCTC	2 cut(s)	577, 1231
BsrDI	GCAATG	1 cut(s)	822
BsrFI	RCCGGY	2 cut(s)	379, 1332
BsrI	ACTGG	7 cut(s)	733, 1037, 1364, 1422, 1451, 1775, 1875
BssAI	RCCGGY	2 cut(s)	379, 1332
BssECI	CCNNGG	9 cut(s)	270, 447, 613, 614, 684, 997, 1885, 2024, 2033
BssNAI	GTATAC	1 cut(s)	2122
BssNI	GRCGYC	2 cut(s)	377, 402
BssSI	CACGAG	1 cut(s)	1794
BssT1I	CCWWGG	3 cut(s)	270, 997, 2024
Bst1107I	GTATAC	1 cut(s)	2122
Bst2BI	CACGAG	1 cut(s)	1794
Bst2UI	CCWGG	3 cut(s)	615, 1887, 2034
Bst6I	CTCTTC	4 cut(s)	17, 885, 1662, 2151
BstACI	GRCGYC	2 cut(s)	377, 402
BstAPI	GCANNNNNTGC	2 cut(s)	1578, 2050
BstBI	TTCGAA	1 cut(s)	1893
BstC8I	GCNNGC	7 cut(s)	381, 1276, 1376, 1448, 1474, 1478, 1882
BstDEI	CTNAG	1 cut(s)	1022
BstDSI	CCRYGG	2 cut(s)	270, 684
BstF5I	GGATG	5 cut(s)	892, 1041, 1276, 1805, 1837
BstFNI	CGCG	2 cut(s)	164, 686
BstH2I	RGCGCY	1 cut(s)	380
BstHHI	GCGC	4 cut(s)	180, 379, 970, 1583
BstMAI	GTCTC	5 cut(s)	38, 577, 1202, 1231, 1659
BstMWI	GCNNNNNNNGC	6 cut(s)	374, 584, 593, 1578, 1587, 2050
BstNI	CCWGG	3 cut(s)	615, 1887, 2034
BstSCI	CCNGG	6 cut(s)	284, 613, 1410, 1885, 2032, 2077
BstSFI	CTRYAG	4 cut(s)	426, 1464, 1860, 2151
BstUI	CGCG	2 cut(s)	164, 686
BstV1I	GCAGC	5 cut(s)	380, 481, 799, 1577, 1846
BstV2I	GAAGAC	3 cut(s)	397, 674, 1813
BstX2I	RGATCY	2 cut(s)	916, 2020
BstXI	CCANNNNNNTGG	1 cut(s)	1359
BstYI	RGATCY	2 cut(s)	916, 2020
BstZ17I	GTATAC	1 cut(s)	2122
BsuI	GTATCC	2 cut(s)	620, 660
BtgI	CCRYGG	2 cut(s)	270, 684
BtsCI	GGATG	5 cut(s)	892, 1041, 1276, 1805, 1837
BtsI	GCAGTG	1 cut(s)	2173
BtsIMutI	CAGTG	4 cut(s)	879, 1458, 2055, 2173
BveI	ACCTGC	2 cut(s)	697, 1471
Cac8I	GCNNGC	7 cut(s)	381, 1276, 1376, 1448, 1474, 1478, 1882
CaiI	CAGNNNCTG	2 cut(s)	812, 1886
CfoI	GCGC	4 cut(s)	180, 379, 970, 1583
Cfr10I	RCCGGY	2 cut(s)	379, 1332
Cfr42I	CCGCGG	1 cut(s)	687
CseI	GACGC	2 cut(s)	391, 866
Csp6I	GTAC	7 cut(s)	565, 603, 945, 1119, 1248, 1865, 1903
CspAI	ACCGGT	1 cut(s)	1332
CviAII	CATG	6 cut(s)	271, 304, 763, 830, 1569, 1957
CviQI	GTAC	7 cut(s)	565, 603, 945, 1119, 1248, 1865, 1903
DdeI	CTNAG	1 cut(s)	1022
DinI	GGCGCC	1 cut(s)	378
DraI	TTTAAA	2 cut(s)	868, 1312
DraIII	CACNNNGTG	1 cut(s)	1742
EaeI	YGGCCR	3 cut(s)	594, 783, 1169
Eam1104I	CTCTTC	4 cut(s)	17, 885, 1662, 2151
EarI	CTCTTC	4 cut(s)	17, 885, 1662, 2151
EciI	GGCGGA	1 cut(s)	2006
Eco130I	CCWWGG	3 cut(s)	270, 997, 2024
Eco24I	GRGCYC	2 cut(s)	284, 436
Eco31I	GGTCTC	2 cut(s)	577, 1231
Eco47I	GGWCC	4 cut(s)	59, 729, 825, 1335
Eco57I	CTGAAG	1 cut(s)	686
Eco88I	CYCGRG	3 cut(s)	277, 446, 948
EcoO109I	RGGNCCY	5 cut(s)	59, 729, 825, 1883, 1964
EcoRII	CCWGG	3 cut(s)	613, 1885, 2032
EcoT14I	CCWWGG	3 cut(s)	270, 997, 2024
EcoT38I	GRGCYC	2 cut(s)	284, 436
EgeI	GGCGCC	1 cut(s)	378
EheI	GGCGCC	1 cut(s)	378
ErhI	CCWWGG	3 cut(s)	270, 997, 2024
FaeI	CATG	6 cut(s)	274, 307, 766, 833, 1572, 1960
FaqI	GGGAC	7 cut(s)	704, 742, 838, 1043, 1321, 1404, 1550
FatI	CATG	6 cut(s)	270, 303, 762, 829, 1568, 1956
FauI	CCCGC	2 cut(s)	450, 679
FbaI	TGATCA	1 cut(s)	939
FblI	GTMKAC	2 cut(s)	855, 2121
Fnu4HI	GCNGC	8 cut(s)	366, 369, 470, 810, 813, 1591, 1860, 2237
FokI	GGATG	5 cut(s)	899, 1048, 1283, 1812, 1844
FriOI	GRGCYC	2 cut(s)	284, 436
Fsp4HI	GCNGC	8 cut(s)	366, 369, 470, 810, 813, 1591, 1860, 2237
FspBI	CTAG	6 cut(s)	606, 1136, 1521, 1596, 2025, 2163
GlaI	GCGC	4 cut(s)	179, 378, 969, 1582
GluI	GCNGC	8 cut(s)	366, 369, 470, 810, 813, 1591, 1860, 2237
GsaI	CCCAGC	6 cut(s)	358, 439, 648, 809, 1030, 1342
HaeII	RGCGCY	1 cut(s)	380
HapII	CCGG	6 cut(s)	286, 374, 380, 1333, 1411, 2079
HgaI	GACGC	2 cut(s)	391, 866
HhaI	GCGC	4 cut(s)	180, 379, 970, 1583
Hin1I	GRCGYC	2 cut(s)	377, 402
Hin1II	CATG	6 cut(s)	274, 307, 766, 833, 1572, 1960
Hin6I	GCGC	4 cut(s)	178, 377, 968, 1581
HinP1I	GCGC	4 cut(s)	178, 377, 968, 1581
HpaII	CCGG	6 cut(s)	286, 374, 380, 1333, 1411, 2079
HphI	GGTGA	4 cut(s)	1338, 1426, 1757, 2242
Hpy166II	GTNNAC	5 cut(s)	552, 856, 1112, 2061, 2122
Hpy188I	TCNGA	7 cut(s)	300, 388, 626, 654, 666, 1096, 2112
Hpy188III	TCNNGA	4 cut(s)	727, 1007, 1136, 1297
Hpy8I	GTNNAC	5 cut(s)	552, 856, 1112, 2061, 2122
Hpy99I	CGWCG	3 cut(s)	521, 602, 2116
HpyAV	CCTTC	4 cut(s)	352, 777, 1196, 2011
HpyCH4IV	ACGT	1 cut(s)	98
HpyF10VI	GCNNNNNNNGC	6 cut(s)	374, 584, 593, 1578, 1587, 2050
HpyF3I	CTNAG	1 cut(s)	1022
HpySE526I	ACGT	1 cut(s)	98
Hsp92I	GRCGYC	2 cut(s)	377, 402
Hsp92II	CATG	6 cut(s)	274, 307, 766, 833, 1572, 1960
HspAI	GCGC	4 cut(s)	178, 377, 968, 1581
KasI	GGCGCC	1 cut(s)	376
KflI	GGGWCCC	2 cut(s)	729, 825
KroI	GCCGGC	1 cut(s)	379
KroNI	GCCGGC	1 cut(s)	381
Ksp22I	TGATCA	1 cut(s)	939
KspI	CCGCGG	1 cut(s)	687
LmnI	GCTCC	6 cut(s)	287, 376, 431, 444, 1511, 2003
Lsp1109I	GCAGC	5 cut(s)	380, 481, 799, 1577, 1846
LweI	GCATC	5 cut(s)	693, 1261, 1290, 1773, 2223
MaeI	CTAG	6 cut(s)	606, 1136, 1521, 1596, 2025, 2163
MaeII	ACGT	1 cut(s)	98
MaeIII	GTNAC	5 cut(s)	99, 973, 1813, 1919, 2171
MfeI	CAATTG	1 cut(s)	1825
MflI	RGATCY	2 cut(s)	916, 2020
MhlI	GDGCHC	5 cut(s)	284, 436, 504, 2055, 2144
MlsI	TGGCCA	1 cut(s)	785
MluI	ACGCGT	1 cut(s)	162
MluNI	TGGCCA	1 cut(s)	785
Mly113I	GGCGCC	1 cut(s)	377
MlyI	GAGTC	6 cut(s)	269, 383, 494, 1426, 1565, 1821
MmeI	TCCRAC	5 cut(s)	119, 411, 604, 1217, 2135
Mox20I	TGGCCA	1 cut(s)	785
MroNI	GCCGGC	1 cut(s)	379
MscI	TGGCCA	1 cut(s)	785
MseI	TTAA	7 cut(s)	678, 867, 1311, 1710, 1911, 1932, 2127
MslI	CAYNNNNRTG	1 cut(s)	881
Msp20I	TGGCCA	1 cut(s)	785
MspA1I	CMGCKG	3 cut(s)	686, 809, 1476
MspI	CCGG	6 cut(s)	286, 374, 380, 1333, 1411, 2079
MspR9I	CCNGG	6 cut(s)	286, 615, 1412, 1887, 2034, 2079
MunI	CAATTG	1 cut(s)	1825
MvaI	CCWGG	3 cut(s)	615, 1887, 2034
MvnI	CGCG	2 cut(s)	164, 686
MwoI	GCNNNNNNNGC	6 cut(s)	374, 584, 593, 1578, 1587, 2050
NaeI	GCCGGC	1 cut(s)	381
NarI	GGCGCC	1 cut(s)	377
NciI	CCSGG	3 cut(s)	286, 1412, 2079
NcoI	CCATGG	1 cut(s)	270
NgoMIV	GCCGGC	1 cut(s)	379
NlaIII	CATG	6 cut(s)	274, 307, 766, 833, 1572, 1960
NmuCI	GTSAC	3 cut(s)	973, 1813, 1919
NspV	TTCGAA	1 cut(s)	1893
PasI	CCCWGGG	1 cut(s)	614
PcsI	WCGNNNNNNNCGW	1 cut(s)	516
PdiI	GCCGGC	1 cut(s)	381
PfeI	GAWTC	5 cut(s)	5, 1088, 1132, 1365, 2082
PflMI	CCANNNNNTGG	1 cut(s)	243
PfoI	TCCNGGA	2 cut(s)	284, 2077
PinAI	ACCGGT	1 cut(s)	1332
PkrI	GCNGC	8 cut(s)	367, 370, 471, 811, 814, 1592, 1861, 2238
PleI	GAGTC	6 cut(s)	269, 383, 493, 1425, 1564, 1820
PluTI	GGCGCC	1 cut(s)	380
PpsI	GAGTC	6 cut(s)	269, 383, 493, 1425, 1564, 1820
PpuMI	RGGWCCY	3 cut(s)	59, 729, 825
PshBI	ATTAAT	1 cut(s)	1932
PsiI	TTATAA	1 cut(s)	1392
Psp1406I	AACGTT	1 cut(s)	98
Psp5II	RGGWCCY	3 cut(s)	59, 729, 825
Psp6I	CCWGG	3 cut(s)	613, 1885, 2032
PspFI	CCCAGC	6 cut(s)	354, 435, 644, 805, 1026, 1338
PspGI	CCWGG	3 cut(s)	613, 1885, 2032
PspPPI	RGGWCCY	3 cut(s)	59, 729, 825
PstI	CTGCAG	2 cut(s)	1468, 1864
PstNI	CAGNNNCTG	2 cut(s)	812, 1886
PsuI	RGATCY	2 cut(s)	916, 2020
PvuII	CAGCTG	1 cut(s)	1476
RsaI	GTAC	7 cut(s)	566, 604, 946, 1120, 1249, 1866, 1904
RsaNI	GTAC	7 cut(s)	565, 603, 945, 1119, 1248, 1865, 1903
RseI	CAYNNNNRTG	1 cut(s)	881
SacII	CCGCGG	1 cut(s)	687
SaqAI	TTAA	7 cut(s)	678, 867, 1311, 1710, 1911, 1932, 2127
SatI	GCNGC	8 cut(s)	366, 369, 470, 810, 813, 1591, 1860, 2237
ScaI	AGTACT	2 cut(s)	566, 946
SchI	GAGTC	6 cut(s)	269, 383, 494, 1426, 1565, 1821
ScrFI	CCNGG	6 cut(s)	286, 615, 1412, 1887, 2034, 2079
SduI	GDGCHC	5 cut(s)	284, 436, 504, 2055, 2144
SfaNI	GCATC	5 cut(s)	693, 1261, 1290, 1773, 2223
SfcI	CTRYAG	4 cut(s)	426, 1464, 1860, 2151
SfoI	GGCGCC	1 cut(s)	378
Sfr303I	CCGCGG	1 cut(s)	687
SfuI	TTCGAA	1 cut(s)	1893
SgrBI	CCGCGG	1 cut(s)	687
SinI	GGWCC	4 cut(s)	59, 729, 825, 1335
SmiMI	CAYNNNNRTG	1 cut(s)	881
SmlI	CTYRAG	1 cut(s)	524
SmoI	CTYRAG	1 cut(s)	524
SpeI	ACTAGT	1 cut(s)	605
SsiI	CCGC	8 cut(s)	363, 366, 443, 674, 684, 686, 809, 1991
SspDI	GGCGCC	1 cut(s)	376
SspI	AATATT	2 cut(s)	775, 1495
SspMI	CTAG	6 cut(s)	606, 1136, 1521, 1596, 2025, 2163
StyD4I	CCNGG	6 cut(s)	284, 613, 1410, 1885, 2032, 2077
StyI	CCWWGG	3 cut(s)	270, 997, 2024
TaiI	ACGT	1 cut(s)	101
TaqI	TCGA	3 cut(s)	516, 1609, 1893
TaqII	GACCGA	2 cut(s)	674, 1452
TatI	WGTACW	3 cut(s)	564, 944, 1864
TauI	GCSGC	2 cut(s)	368, 812
TfiI	GAWTC	5 cut(s)	5, 1088, 1132, 1365, 2082
Tru1I	TTAA	7 cut(s)	678, 867, 1311, 1710, 1911, 1932, 2127
Tru9I	TTAA	7 cut(s)	678, 867, 1311, 1710, 1911, 1932, 2127
TscAI	CASTG	4 cut(s)	886, 1458, 2055, 2173
TseFI	GTSAC	3 cut(s)	973, 1813, 1919
TseI	GCWGC	6 cut(s)	368, 469, 812, 1590, 1859, 2236
Tsp45I	GTSAC	3 cut(s)	973, 1813, 1919
TspDTI	ATGAA	7 cut(s)	846, 903, 1039, 1278, 1524, 1848, 1945
TspRI	CASTG	4 cut(s)	886, 1458, 2055, 2173
Van91I	CCANNNNNTGG	1 cut(s)	243
VpaK11BI	GGWCC	4 cut(s)	59, 729, 825, 1335
VspI	ATTAAT	1 cut(s)	1932
XapI	RAATTY	2 cut(s)	225, 862
XbaI	TCTAGA	1 cut(s)	1135
XcmI	CCANNNNNNNNNTGG	3 cut(s)	589, 778, 995
XmaJI	CCTAGG	1 cut(s)	2024
XmiI	GTMKAC	2 cut(s)	855, 2121
XspI	CTAG	6 cut(s)	606, 1136, 1521, 1596, 2025, 2163
ZrmI	AGTACT	2 cut(s)	566, 946

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.