MD13G1168000.v1.1

CLASP N terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0014024
Physical Location & Seq
Forward (+)
13606399 .. 13607965
1567 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1168000.v1.1.491

Sequence Viewer

Length: 933 bp
ATGGCAATGGCTCTCAGACCCATCGACAATGCTCTCCCAACCACACCGGAAAGACCCAAAAAGCAAACCAAAGTTGCTGTTCCTATGCAAAAGCAGCAATCCGGCCTCGTAAATGACGAAAATCAGGCTCCGCTTCCTGCAACCGCGGATTTCGCCATCGATTACATCTCCTCTGAGAATCTCAAACCCATCTCAGACCCGGATCTCAAGATCCAGAGCTTGAGTGAGGGATTCGAATCAAAGGATTGGGTAAAGGTGTGTGAGTCTCTGAACAATGTTAGGCGTTTTGCACTCCACCATTCTGCTCTTCTGGCTCCTACTCTAGAGAATGTTGTAGTGGTGCTGGTGAAGGCAATGAAGAATCCGAGAAGTGCCTTAATCAAGACCTCGATTATGGCTTCTTCCGACATCTTCACCGCCTTTGGTGATGGTTTACTTGACTCCGCAGCCTCTAATGCATTTGACCAATTGCTGCTGCAATTGCTGCTCAAGGCATCTCAAGACAAACGTTTTGTGTGTGAAGAAGCAGACAGGGCACTGAGCTCAATGGTGCAGTCCTTGACCCCTCTTCCTCTGCTTCAGAAGCTCCGAGCTTACGCTTCCCATGGCAACCCCAGAGTCAGAGCCAAAGCTGCCGTTTCCATCGCATCTTGTGTCGCCAAGATGGGCCTTGAAGGATTGAAGGATTATGGGCTGGTTTCTCTGGTACAAAAGGCTGCAGATTTGTTGAATGATAGGCTGCCCGAGGCGAGAGAAGCAGCGCGGGGAATTGTGATGTCGGTATACAATGCCTATACAGAGAATGAAGAGGAGAAGCTGCGGGAAAATGAAGAGGAGAAGCCGGAGAAGCAGGAGATTTGGTTAAGCTTTTGCCAGTCTAATTTGTCAACAATTCATGCTCAGTCCATGGTCAAAATTACTTCTTCTCAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLASP_N

Protein Analysis

311

Amino Acids

34.11

Weight (kDa)

6.16

Isoelectric Point (pI)

47.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CLASP_N PF12348 109 - 273 4.1e-06 CLASP N terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014024)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15830
fragaria_vesca FvH4_4g18210
malus_domestica MD13G1168000.v1.1 MD16G1168400.v1.1
prunus_persica Prupe.1G135600_v2.0.a1
pyrus_communis pycom13g14380 pycom16g14180
rosa_chinensis RchiOBHm_Chr4g0422361
rosa_laevigata RLG00000007616
rosa_multiflora Rmu_co8367675.1_g000001 Rmu_sc0013245.1_g000010
rosa_roxburghii Rroxscaffold_5G00364480
rosa_rugosa Rorug04G0179400
rosa_samantha Rh4AG238800 Rh4BG242400 Rh4CG253900
rosa_wichuraiana Rw4G020650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 783
AccII CGCG 2 cut(s) 146, 763
AciI CCGC 7 cut(s) 131, 144, 146, 417, 444, 763, 820
AclI AACGTT 1 cut(s) 508
AclWI GGATC 2 cut(s) 205, 210
AcuI CTGAAG 1 cut(s) 563
AfaI GTAC 1 cut(s) 708
AgsI TTSAA 3 cut(s) 674, 682, 730
AluBI AGCT 7 cut(s) 219, 543, 586, 593, 632, 817, 867
AluI AGCT 7 cut(s) 219, 543, 586, 593, 632, 817, 867
Alw21I GWGCWC 1 cut(s) 545
Alw26I GTCTC 1 cut(s) 270
AlwI GGATC 2 cut(s) 205, 210
Ama87I CYCGRG 1 cut(s) 743
AoxI GGCC 2 cut(s) 103, 667
ArsI GACNNNNNNTTYG 2 cut(s) 494, 526
AspLEI GCGC 1 cut(s) 763
AspS9I GGNCC 1 cut(s) 667
AsuC2I CCSGG 1 cut(s) 200
AsuHPI GGTGA 3 cut(s) 358, 406, 437
AsuII TTCGAA 1 cut(s) 234
AvaI CYCGRG 1 cut(s) 743
BaeGI GKGCMC 1 cut(s) 538
BanII GRGCYC 1 cut(s) 545
Bbv12I GWGCWC 1 cut(s) 545
BccI CCATC 6 cut(s) 29, 164, 197, 422, 650, 658
BceAI ACGGC 1 cut(s) 620
BcnI CCSGG 1 cut(s) 200
BcoDI GTCTC 1 cut(s) 270
BfaI CTAG 1 cut(s) 323
BfmI CTRYAG 1 cut(s) 717
Bme1390I CCNGG 1 cut(s) 200
BmeT110I CYCGRG 1 cut(s) 743
BmgT120I GGNCC 1 cut(s) 667
BmiI GGNNCC 2 cut(s) 129, 315
BmrFI CCNGG 1 cut(s) 200
BmsI GCATC 2 cut(s) 503, 656
Bpu14I TTCGAA 1 cut(s) 234
BpuEI CTTGAG 4 cut(s) 191, 241, 473, 483
BpuMI CCSGG 1 cut(s) 200
Bsa29I ATCGAT 1 cut(s) 159
BsaBI GATNNNNATC 1 cut(s) 235
BsaJI CCNNGG 4 cut(s) 144, 604, 744, 906
BsaWI WCCGGW 1 cut(s) 46
BsaXI ACNNNNNCTCC 2 cut(s) 845, 875
Bse1I ACTGG 1 cut(s) 874
Bse3DI GCAATG 2 cut(s) 12, 360
Bse8I GATNNNNATC 1 cut(s) 235
BseCI ATCGAT 1 cut(s) 159
BseDI CCNNGG 4 cut(s) 144, 604, 744, 906
BseJI GATNNNNATC 1 cut(s) 235
BseMI GCAATG 2 cut(s) 12, 360
BseMII CTCAG 5 cut(s) 28, 165, 207, 530, 914
BseNI ACTGG 1 cut(s) 874
BseRI GAGGAG 3 cut(s) 160, 824, 848
BseSI GKGCMC 1 cut(s) 538
BsgI GTGCAG 1 cut(s) 572
Bsh1236I CGCG 2 cut(s) 146, 763
BshFI GGCC 2 cut(s) 105, 669
BshVI ATCGAT 1 cut(s) 159
BsiHKAI GWGCWC 1 cut(s) 545
BsiHKCI CYCGRG 1 cut(s) 743
BsiSI CCGG 4 cut(s) 47, 102, 200, 842
BsmAI GTCTC 1 cut(s) 270
BsnI GGCC 2 cut(s) 105, 669
BsoBI CYCGRG 1 cut(s) 743
Bsp119I TTCGAA 1 cut(s) 234
Bsp1286I GDGCHC 2 cut(s) 538, 545
Bsp143I GATC 2 cut(s) 202, 210
Bsp19I CCATGG 2 cut(s) 604, 906
BspACI CCGC 7 cut(s) 131, 144, 146, 417, 444, 763, 820
BspANI GGCC 2 cut(s) 105, 669
BspCNI CTCAG 5 cut(s) 27, 166, 206, 531, 913
BspDI ATCGAT 1 cut(s) 159
BspFNI CGCG 2 cut(s) 146, 763
BspLI GGNNCC 2 cut(s) 129, 315
BspMAI CTGCAG 1 cut(s) 721
BspPI GGATC 2 cut(s) 205, 210
BspQI GCTCTTC 1 cut(s) 312
BspT104I TTCGAA 1 cut(s) 234
BsrDI GCAATG 2 cut(s) 12, 360
BsrI ACTGG 1 cut(s) 874
BssECI CCNNGG 4 cut(s) 144, 604, 744, 906
BssMI GATC 2 cut(s) 202, 210
BssNAI GTATAC 1 cut(s) 784
BssT1I CCWWGG 2 cut(s) 604, 906
Bst1107I GTATAC 1 cut(s) 784
Bst6I CTCTTC 4 cut(s) 312, 573, 801, 825
BstAPI GCANNNNNTGC 1 cut(s) 484
BstBI TTCGAA 1 cut(s) 234
BstDEI CTNAG 6 cut(s) 14, 174, 193, 539, 900, 927
BstDSI CCRYGG 3 cut(s) 144, 604, 906
BstFNI CGCG 2 cut(s) 146, 763
BstHHI GCGC 1 cut(s) 763
BstKTI GATC 2 cut(s) 205, 213
BstMAI GTCTC 1 cut(s) 270
BstMBI GATC 2 cut(s) 202, 210
BstSCI CCNGG 1 cut(s) 198
BstSFI CTRYAG 1 cut(s) 717
BstSLI GKGCMC 1 cut(s) 538
BstUI CGCG 2 cut(s) 146, 763
BstX2I RGATCY 2 cut(s) 202, 210
BstYI RGATCY 2 cut(s) 202, 210
BstZ17I GTATAC 1 cut(s) 784
Bsu15I ATCGAT 1 cut(s) 159
BsuRI GGCC 2 cut(s) 105, 669
BsuTUI ATCGAT 1 cut(s) 159
BtgI CCRYGG 3 cut(s) 144, 604, 906
BtgZI GCGATG 1 cut(s) 628
BtsIMutI CAGTG 1 cut(s) 536
CfoI GCGC 1 cut(s) 763
Cfr13I GGNCC 1 cut(s) 667
Cfr42I CCGCGG 1 cut(s) 147
ClaI ATCGAT 1 cut(s) 159
Csp6I GTAC 1 cut(s) 707
CviAII CATG 3 cut(s) 605, 896, 907
CviQI GTAC 1 cut(s) 707
DdeI CTNAG 6 cut(s) 14, 174, 193, 539, 900, 927
DpnI GATC 2 cut(s) 204, 212
DpnII GATC 2 cut(s) 202, 210
Eam1104I CTCTTC 4 cut(s) 312, 573, 801, 825
EarI CTCTTC 4 cut(s) 312, 573, 801, 825
Ecl136II GAGCTC 1 cut(s) 543
Eco130I CCWWGG 2 cut(s) 604, 906
Eco24I GRGCYC 1 cut(s) 545
Eco53kI GAGCTC 1 cut(s) 543
Eco57I CTGAAG 1 cut(s) 563
Eco88I CYCGRG 1 cut(s) 743
EcoICRI GAGCTC 1 cut(s) 543
EcoT14I CCWWGG 2 cut(s) 604, 906
EcoT22I ATGCAT 1 cut(s) 460
EcoT38I GRGCYC 1 cut(s) 545
ErhI CCWWGG 2 cut(s) 604, 906
FaeI CATG 3 cut(s) 608, 899, 910
FaiI YATR 8 cut(s) 86, 395, 606, 690, 784, 795, 897, 908
FatI CATG 3 cut(s) 604, 895, 906
FauI CCCGC 2 cut(s) 756, 813
FblI GTMKAC 1 cut(s) 783
FriOI GRGCYC 1 cut(s) 545
FspBI CTAG 1 cut(s) 323
GlaI GCGC 1 cut(s) 762
HaeIII GGCC 2 cut(s) 105, 669
HapII CCGG 4 cut(s) 47, 102, 200, 842
HhaI GCGC 1 cut(s) 763
Hin1II CATG 3 cut(s) 608, 899, 910
Hin6I GCGC 1 cut(s) 761
HinP1I GCGC 1 cut(s) 761
HincII GTYRAC 1 cut(s) 888
HindII GTYRAC 1 cut(s) 888
HindIII AAGCTT 1 cut(s) 865
HinfI GANTC 7 cut(s) 178, 231, 236, 263, 361, 440, 618
HpaII CCGG 4 cut(s) 47, 102, 200, 842
HphI GGTGA 3 cut(s) 358, 406, 437
Hpy166II GTNNAC 3 cut(s) 434, 784, 888
Hpy188I TCNGA 9 cut(s) 17, 175, 196, 270, 366, 406, 582, 590, 623
Hpy188III TCNNGA 5 cut(s) 208, 214, 323, 382, 500
Hpy8I GTNNAC 3 cut(s) 434, 784, 888
HpyAV CCTTC 3 cut(s) 343, 668, 676
HpyCH4IV ACGT 1 cut(s) 508
HpyCH4V TGCA 7 cut(s) 88, 140, 290, 458, 478, 553, 719
HpyF3I CTNAG 6 cut(s) 14, 174, 193, 539, 900, 927
HpySE526I ACGT 1 cut(s) 508
Hsp92II CATG 3 cut(s) 608, 899, 910
HspAI GCGC 1 cut(s) 761
KspI CCGCGG 1 cut(s) 147
Kzo9I GATC 2 cut(s) 202, 210
LguI GCTCTTC 1 cut(s) 312
LmnI GCTCC 3 cut(s) 133, 319, 591
LweI GCATC 2 cut(s) 503, 656
MaeI CTAG 1 cut(s) 323
MaeII ACGT 1 cut(s) 508
MalI GATC 2 cut(s) 204, 212
MboI GATC 2 cut(s) 202, 210
MboII GAAGA 9 cut(s) 299, 370, 393, 403, 533, 560, 818, 842, 915
MfeI CAATTG 2 cut(s) 467, 479
MflI RGATCY 2 cut(s) 202, 210
MhlI GDGCHC 2 cut(s) 538, 545
MluCI AATT 6 cut(s) 467, 479, 768, 880, 891, 915
MlyI GAGTC 3 cut(s) 272, 434, 627
MmeI TCCRAC 1 cut(s) 429
Mph1103I ATGCAT 1 cut(s) 460
MseI TTAA 2 cut(s) 377, 863
MspA1I CMGCKG 1 cut(s) 146
MspI CCGG 4 cut(s) 47, 102, 200, 842
MspR9I CCNGG 1 cut(s) 200
MunI CAATTG 2 cut(s) 467, 479
MvnI CGCG 2 cut(s) 146, 763
NciI CCSGG 1 cut(s) 200
NcoI CCATGG 2 cut(s) 604, 906
NdeII GATC 2 cut(s) 202, 210
NlaIII CATG 3 cut(s) 608, 899, 910
NlaIV GGNNCC 2 cut(s) 129, 315
NsiI ATGCAT 1 cut(s) 460
NspV TTCGAA 1 cut(s) 234
PciSI GCTCTTC 1 cut(s) 312
PcsI WCGNNNNNNNCGW 1 cut(s) 114
PfeI GAWTC 4 cut(s) 178, 231, 236, 361
PleI GAGTC 3 cut(s) 271, 434, 626
PpsI GAGTC 3 cut(s) 271, 434, 626
Psp124BI GAGCTC 1 cut(s) 545
Psp1406I AACGTT 1 cut(s) 508
PspN4I GGNNCC 2 cut(s) 129, 315
PspPI GGNCC 1 cut(s) 667
PstI CTGCAG 1 cut(s) 721
PsuI RGATCY 2 cut(s) 202, 210
RsaI GTAC 1 cut(s) 708
RsaNI GTAC 1 cut(s) 707
SacI GAGCTC 1 cut(s) 545
SacII CCGCGG 1 cut(s) 147
SapI GCTCTTC 1 cut(s) 312
SaqAI TTAA 2 cut(s) 377, 863
Sau3AI GATC 2 cut(s) 202, 210
Sau96I GGNCC 1 cut(s) 667
SchI GAGTC 3 cut(s) 272, 434, 627
ScrFI CCNGG 1 cut(s) 200
SduI GDGCHC 2 cut(s) 538, 545
SfaNI GCATC 2 cut(s) 503, 656
SfcI CTRYAG 1 cut(s) 717
Sfr303I CCGCGG 1 cut(s) 147
SfuI TTCGAA 1 cut(s) 234
SgrBI CCGCGG 1 cut(s) 147
SmlI CTYRAG 4 cut(s) 206, 220, 488, 498
SmoI CTYRAG 4 cut(s) 206, 220, 488, 498
Sse9I AATT 6 cut(s) 467, 479, 768, 880, 891, 915
SsiI CCGC 7 cut(s) 131, 144, 146, 417, 444, 763, 820
SspMI CTAG 1 cut(s) 323
SstI GAGCTC 1 cut(s) 545
StyD4I CCNGG 1 cut(s) 198
StyI CCWWGG 2 cut(s) 604, 906
TaiI ACGT 1 cut(s) 511
TaqI TCGA 4 cut(s) 24, 159, 234, 389
TasI AATT 6 cut(s) 467, 479, 768, 880, 891, 915
TfiI GAWTC 4 cut(s) 178, 231, 236, 361
Tru1I TTAA 2 cut(s) 377, 863
Tru9I TTAA 2 cut(s) 377, 863
TscAI CASTG 1 cut(s) 543
TspDTI ATGAA 4 cut(s) 371, 819, 843, 884
TspRI CASTG 1 cut(s) 543
XbaI TCTAGA 1 cut(s) 322
XmiI GTMKAC 1 cut(s) 783
XspI CTAG 1 cut(s) 323
Zsp2I ATGCAT 1 cut(s) 460
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.