Rorug04G0179400

CLASP N terminal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0014024
Physical Location & Seq
Forward (+)
31477209 .. 31478234
1026 bp
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UTR
Exon/CDS
Intron
Rorug04G0179400.1

Sequence Viewer

Length: 474 bp
ATGGGTGTCTTCAAATACGAGTCCGAATTCACCTCTGTTATTCCCCCAGCTAGGTTGTTCAAGGCCTTCGTTCTCGATGCTGACAACCTCATCCCCAAGATTGCACCACAGGCAGTTCAGTCCGCTGAGATCCTTGAAGGAGATGGTGGTGCTGGAACCATCAAGAAGATCACCTTCGGCGAAGGTAGCACTTACAGCTATGTGAAGCACAGAATTGATGGCATTGACAAGGAGAACTTTGTCTACAGCTACAGTGTGATTGAGGGAGCTCCTGACTCAATCGAGAAGATCTGCTACGAGACTAAGTTGGTGGCATCCGGCAGTGGTACCGTAATCAAGAGCACCAGTGAATACCATGTCAAGGGTGATGTTGAGATCAAGGAAGAGCACGTCAAGGCCGGGAAAGAGAAGGCTTCTCACCTCTTCAAGGTCATTGAGAACTACCTCTTGGAGCACCATGATGCCTACAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CLASP_N

Protein Analysis

157

Amino Acids

17.33

Weight (kDa)

5.51

Isoelectric Point (pI)

22.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Bet_v_1 PF00407 1 - 152 2.9e-22 Pathogenesis-related protein Bet v 1 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014024)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G15830
fragaria_vesca FvH4_4g18210
malus_domestica MD13G1168000.v1.1 MD16G1168400.v1.1
prunus_persica Prupe.1G135600_v2.0.a1
pyrus_communis pycom13g14380 pycom16g14180
rosa_chinensis RchiOBHm_Chr4g0422361
rosa_laevigata RLG00000007616
rosa_multiflora Rmu_co8367675.1_g000001 Rmu_sc0013245.1_g000010
rosa_roxburghii Rroxscaffold_5G00364480
rosa_rugosa Rorug04G0179400
rosa_samantha Rh4AG238800 Rh4BG242400 Rh4CG253900
rosa_wichuraiana Rw4G020650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 326
AccB1I GGYRCC 1 cut(s) 326
AccI GTMKAC 1 cut(s) 243
AciI CCGC 1 cut(s) 123
AclWI GGATC 1 cut(s) 124
AcsI RAATTY 1 cut(s) 26
AfaI GTAC 1 cut(s) 328
AfiI CCNNNNNNNGG 3 cut(s) 51, 361, 427
AgsI TTSAA 4 cut(s) 13, 61, 137, 427
AjiI CACGTC 1 cut(s) 391
AjuI GAANNNNNNNTTGG 2 cut(s) 431, 463
AluBI AGCT 4 cut(s) 50, 198, 249, 269
AluI AGCT 4 cut(s) 50, 198, 249, 269
Alw21I GWGCWC 4 cut(s) 271, 344, 390, 456
Alw26I GTCTC 1 cut(s) 293
AlwI GGATC 1 cut(s) 124
AoxI GGCC 2 cut(s) 63, 396
ApoI RAATTY 1 cut(s) 26
Asp718I GGTACC 1 cut(s) 326
AsuC2I CCSGG 1 cut(s) 400
AsuHPI GGTGA 4 cut(s) 22, 163, 377, 410
BanI GGYRCC 1 cut(s) 326
BanII GRGCYC 1 cut(s) 271
BarI GAAGNNNNNNTAC 2 cut(s) 278, 310
Bbv12I GWGCWC 4 cut(s) 271, 344, 390, 456
BccI CCATC 3 cut(s) 137, 167, 212
BcnI CCSGG 1 cut(s) 400
BcoDI GTCTC 1 cut(s) 293
BfaI CTAG 1 cut(s) 51
BfmI CTRYAG 2 cut(s) 244, 250
BglII AGATCT 1 cut(s) 288
Bme1390I CCNGG 1 cut(s) 400
BmgBI CACGTC 1 cut(s) 391
BmiI GGNNCC 2 cut(s) 157, 328
BmrFI CCNGG 1 cut(s) 400
BmsI GCATC 3 cut(s) 67, 323, 451
BpuMI CCSGG 1 cut(s) 400
Bsc4I CCNNNNNNNGG 3 cut(s) 51, 361, 427
Bse1I ACTGG 1 cut(s) 345
BseGI GGATG 2 cut(s) 90, 314
BseLI CCNNNNNNNGG 3 cut(s) 51, 361, 427
BseMII CTCAG 1 cut(s) 117
BseNI ACTGG 1 cut(s) 345
BseYI CCCAGC 1 cut(s) 46
BshFI GGCC 2 cut(s) 65, 398
BshNI GGYRCC 1 cut(s) 326
BsiHKAI GWGCWC 4 cut(s) 271, 344, 390, 456
BsiSI CCGG 2 cut(s) 318, 399
BslI CCNNNNNNNGG 3 cut(s) 51, 361, 427
BsmAI GTCTC 1 cut(s) 293
BsnI GGCC 2 cut(s) 65, 398
Bsp1286I GDGCHC 4 cut(s) 271, 344, 390, 456
Bsp143I GATC 4 cut(s) 129, 168, 288, 375
BspACI CCGC 1 cut(s) 123
BspANI GGCC 2 cut(s) 65, 398
BspCNI CTCAG 1 cut(s) 118
BspLI GGNNCC 2 cut(s) 157, 328
BspPI GGATC 1 cut(s) 124
BspQI GCTCTTC 1 cut(s) 378
BspT107I GGYRCC 1 cut(s) 326
BsrI ACTGG 1 cut(s) 345
BssMI GATC 4 cut(s) 129, 168, 288, 375
Bst4CI ACNGT 2 cut(s) 254, 331
Bst6I CTCTTC 2 cut(s) 378, 428
BstDEI CTNAG 2 cut(s) 126, 303
BstENI CCTNNNNNAGG 1 cut(s) 425
BstF5I GGATG 2 cut(s) 90, 314
BstKTI GATC 4 cut(s) 132, 171, 291, 378
BstMAI GTCTC 1 cut(s) 293
BstMBI GATC 4 cut(s) 129, 168, 288, 375
BstMWI GCNNNNNNNGC 3 cut(s) 110, 186, 195
BstSCI CCNGG 1 cut(s) 398
BstSFI CTRYAG 2 cut(s) 244, 250
BstX2I RGATCY 2 cut(s) 129, 288
BstYI RGATCY 2 cut(s) 129, 288
BsuRI GGCC 2 cut(s) 65, 398
BtrI CACGTC 1 cut(s) 391
BtsCI GGATG 2 cut(s) 90, 314
BtsI GCAGTG 1 cut(s) 328
BtsIMutI CAGTG 3 cut(s) 259, 328, 352
Csp6I GTAC 1 cut(s) 327
CviAII CATG 2 cut(s) 356, 458
CviJI RGCY 7 cut(s) 50, 65, 198, 249, 269, 398, 413
CviKI_1 RGCY 7 cut(s) 50, 65, 198, 249, 269, 398, 413
CviQI GTAC 1 cut(s) 327
DdeI CTNAG 2 cut(s) 126, 303
DpnI GATC 4 cut(s) 131, 170, 290, 377
DpnII GATC 4 cut(s) 129, 168, 288, 375
Eam1104I CTCTTC 2 cut(s) 378, 428
EarI CTCTTC 2 cut(s) 378, 428
Ecl136II GAGCTC 1 cut(s) 269
Eco147I AGGCCT 1 cut(s) 65
Eco24I GRGCYC 1 cut(s) 271
Eco53kI GAGCTC 1 cut(s) 269
EcoICRI GAGCTC 1 cut(s) 269
EcoNI CCTNNNNNAGG 1 cut(s) 425
EcoRI GAATTC 1 cut(s) 26
EcoT38I GRGCYC 1 cut(s) 271
FaeI CATG 2 cut(s) 359, 461
FaiI YATR 3 cut(s) 201, 357, 459
FalI AAGNNNNNCTT 4 cut(s) 158, 190, 221, 253
FatI CATG 2 cut(s) 355, 457
FblI GTMKAC 1 cut(s) 243
FokI GGATG 2 cut(s) 77, 301
FriOI GRGCYC 1 cut(s) 271
FspBI CTAG 1 cut(s) 51
GsaI CCCAGC 1 cut(s) 50
HaeIII GGCC 2 cut(s) 65, 398
HapII CCGG 2 cut(s) 318, 399
Hin1II CATG 2 cut(s) 359, 461
HinfI GANTC 2 cut(s) 20, 275
HpaII CCGG 2 cut(s) 318, 399
HphI GGTGA 4 cut(s) 22, 163, 377, 410
Hpy166II GTNNAC 1 cut(s) 244
Hpy188I TCNGA 1 cut(s) 25
Hpy188III TCNNGA 5 cut(s) 74, 163, 272, 283, 337
Hpy8I GTNNAC 1 cut(s) 244
HpyAV CCTTC 5 cut(s) 76, 131, 176, 184, 403
HpyCH4III ACNGT 2 cut(s) 254, 331
HpyCH4IV ACGT 1 cut(s) 390
HpyCH4V TGCA 1 cut(s) 104
HpyF10VI GCNNNNNNNGC 3 cut(s) 110, 186, 195
HpyF3I CTNAG 2 cut(s) 126, 303
HpySE526I ACGT 1 cut(s) 390
Hsp92II CATG 2 cut(s) 359, 461
KpnI GGTACC 1 cut(s) 330
Kzo9I GATC 4 cut(s) 129, 168, 288, 375
LguI GCTCTTC 1 cut(s) 378
LmnI GCTCC 3 cut(s) 266, 274, 451
LpnPI CCDG 7 cut(s) 60, 95, 138, 285, 331, 358, 412
LweI GCATC 3 cut(s) 67, 323, 451
MaeI CTAG 1 cut(s) 51
MaeII ACGT 1 cut(s) 390
MalI GATC 4 cut(s) 131, 170, 290, 377
MboI GATC 4 cut(s) 129, 168, 288, 375
MboII GAAGA 4 cut(s) 178, 298, 395, 415
MflI RGATCY 2 cut(s) 129, 288
MhlI GDGCHC 4 cut(s) 271, 344, 390, 456
MluCI AATT 2 cut(s) 26, 213
MlyI GAGTC 2 cut(s) 29, 269
MnlI CCTC 5 cut(s) 43, 98, 256, 431, 455
MslI CAYNNNNRTG 1 cut(s) 459
MspA1I CMGCKG 1 cut(s) 125
MspI CCGG 2 cut(s) 318, 399
MspR9I CCNGG 1 cut(s) 400
MwoI GCNNNNNNNGC 3 cut(s) 110, 186, 195
NciI CCSGG 1 cut(s) 400
NdeII GATC 4 cut(s) 129, 168, 288, 375
NlaIII CATG 2 cut(s) 359, 461
NlaIV GGNNCC 2 cut(s) 157, 328
PceI AGGCCT 1 cut(s) 65
PciSI GCTCTTC 1 cut(s) 378
PleI GAGTC 2 cut(s) 28, 269
PpsI GAGTC 2 cut(s) 28, 269
Psp124BI GAGCTC 1 cut(s) 271
PspFI CCCAGC 1 cut(s) 46
PspN4I GGNNCC 2 cut(s) 157, 328
PsrI GAACNNNNNNTAC 2 cut(s) 227, 259
PsuI RGATCY 2 cut(s) 129, 288
RsaI GTAC 1 cut(s) 328
RsaNI GTAC 1 cut(s) 327
RseI CAYNNNNRTG 1 cut(s) 459
SacI GAGCTC 1 cut(s) 271
SapI GCTCTTC 1 cut(s) 378
Sau3AI GATC 4 cut(s) 129, 168, 288, 375
SchI GAGTC 2 cut(s) 29, 269
ScrFI CCNGG 1 cut(s) 400
SduI GDGCHC 4 cut(s) 271, 344, 390, 456
SfaNI GCATC 3 cut(s) 67, 323, 451
SfcI CTRYAG 2 cut(s) 244, 250
SmiMI CAYNNNNRTG 1 cut(s) 459
Sse9I AATT 2 cut(s) 26, 213
SseBI AGGCCT 1 cut(s) 65
SsiI CCGC 1 cut(s) 123
SspMI CTAG 1 cut(s) 51
SstI GAGCTC 1 cut(s) 271
StuI AGGCCT 1 cut(s) 65
StyD4I CCNGG 1 cut(s) 398
TaaI ACNGT 2 cut(s) 254, 331
TaiI ACGT 1 cut(s) 393
TaqI TCGA 2 cut(s) 75, 282
TasI AATT 2 cut(s) 26, 213
TscAI CASTG 3 cut(s) 259, 328, 352
TspRI CASTG 3 cut(s) 259, 328, 352
XagI CCTNNNNNAGG 1 cut(s) 425
XapI RAATTY 1 cut(s) 26
XmiI GTMKAC 1 cut(s) 243
XspI CTAG 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.