MD13G1182500.v1.1

Serine incorporator

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0011082
Physical Location & Seq
Reverse (-)
15282450 .. 15285889
3440 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1182500.v1.1.491

Sequence Viewer

Length: 1236 bp
ATGTCGTGCTGCTTGTCTTGCTGCGCTGCTTCCACCTGCGGCCTGTGTGGCTCTGTGGCCTCTGGGATTTCCAGCAAATCGGCTAGGCTTGCTTACTGCGGTCTCTTCGGGCTTTCCCTGATCGTGTCTTGGATTCTCAGAGAAGTTGGTGCTCCTCTCTTGGAGAAGATCCCATGGATAAGCTCTTCTGAGACTCACACCAAGGAGTGGTATCAAACAGAAGCGGTTCTTCGTGTGAGCATGGGGAATTTCTTGTTTTTCGCAGCATTTGCGCTTATAATGATTGGAGTAAAGGACCAAAATGACAGACGAGATTCCTGGCATCATGGTGGATGGATTGCCAAGATGGTGGTATGGCTTTTGCTTGTCGTTCTCATGTTTTTCATGCCAGACATTGTCATTACAATCTATGGTGTCGTATCAAAATTTGGGGCAGGCTTATTTTTACTGGTTCAAGTCATTATATTGTTAGACTGTACTCACTCATGGAATGATGCATGGGTGGAAAAAGATGAGCAAAAATGGTATATTGCACTGCTTATCGTATCAATTGTATGCTACCTTGCAGCATTTGCGTTCTCAGGCGTTCTGTTCATTTGGTTCAACCCTTCTGGTGAAGATTGTGGCCTCAATATCTTCTTCATTGTCATGACAATGATTCTCGCATTTGGATTTGCAGTTATTGCGTTACACCCGAAGGTGAATGGCAGCCTCCTCCCTGCTTCGGTGATATCTGTTTATAGTGCTTATGTGTGCTACACGGCTCTTTCCAGTGAACCTCATGGCTATGCTTGCAATGGTCTTCACCATTCGAAAGCAGTCTCCCTGAGTACCCTTCTTCTTGGGATGGCAATGACTGTTCTTTCAGTTTTGTACTCTGCCCTTCGTGCTGGATCATCAACAAATTTTCTATCACCACCATCTTCACCCAGGGGAGGTGCAGCTGAAAAGACACCTCTTTTTGACGGCAAAGAGTTGGAGGAAGGGAAAGAGAAAAATGACAAAGAAGTGAAACCTGTTAGTTATTCCTATACGTTTTTCCACCTGATATTTGCCTTGGCTAGCATGTATTCAGCCATGCTGCTTTCGGGTTGGACCAGCTCATCGGAGAGCTCAGATCTCATTGACGTTGGGTGGACATCGGTTTGGGTCAGAATCTGCACAGAATGGGTTACTGCTGCACTATATGTTTGGTCCCTCATTGCTCCTATACTGATCCCTGATCGTGAGTTCTAG

Protein Analysis

412

Amino Acids

45.16

Weight (kDa)

5.85

Isoelectric Point (pI)

32.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Serinc PF03348 6 - 409 1.6e-120 Serine incorporator (Serinc)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 278
AarI CACCTGC 1 cut(s) 44
Acc36I ACCTGC 1 cut(s) 44
AccB7I CCANNNNNTGG 1 cut(s) 207
AciI CCGC 3 cut(s) 39, 99, 224
AclWI GGATC 3 cut(s) 163, 901, 1210
AcsI RAATTY 3 cut(s) 247, 425, 904
AfaI GTAC 3 cut(s) 478, 832, 875
AfiI CCNNNNNNNGG 3 cut(s) 207, 724, 935
AgsI TTSAA 2 cut(s) 455, 604
AjnI CCWGG 2 cut(s) 317, 929
AluBI AGCT 4 cut(s) 183, 944, 1101, 1113
AluI AGCT 4 cut(s) 183, 944, 1101, 1113
Alw21I GWGCWC 2 cut(s) 154, 1115
Alw26I GTCTC 3 cut(s) 107, 185, 826
AlwI GGATC 3 cut(s) 163, 901, 1210
AlwNI CAGNNNCTG 1 cut(s) 1158
AoxI GGCC 3 cut(s) 40, 57, 625
ApeKI GCWGC 9 cut(s) 9, 21, 26, 263, 566, 708, 941, 1081, 1178
ApoI RAATTY 3 cut(s) 247, 425, 904
Asp700I GAANNNNTTC 1 cut(s) 225
AspLEI GCGC 2 cut(s) 26, 274
AspS9I GGNCC 3 cut(s) 295, 1095, 1194
AsuHPI GGTGA 6 cut(s) 626, 712, 739, 797, 906, 918
AsuII TTCGAA 1 cut(s) 812
AsuNHI GCTAGC 1 cut(s) 1061
AvaII GGWCC 3 cut(s) 295, 1095, 1194
BanII GRGCYC 1 cut(s) 1115
BbsI GAAGAC 1 cut(s) 794
Bbv12I GWGCWC 2 cut(s) 154, 1115
BbvI GCAGC 8 cut(s) 8, 13, 275, 578, 720, 953, 1068, 1165
BccI CCATC 4 cut(s) 327, 340, 841, 928
BceAI ACGGC 2 cut(s) 777, 982
BciT130I CCWGG 2 cut(s) 319, 931
BcoDI GTCTC 3 cut(s) 107, 185, 826
BfaI CTAG 3 cut(s) 84, 1062, 1234
BfuAI ACCTGC 1 cut(s) 44
BglI GCCNNNNNGGC 1 cut(s) 48
BglII AGATCT 1 cut(s) 1117
Bme1390I CCNGG 2 cut(s) 319, 931
Bme18I GGWCC 3 cut(s) 295, 1095, 1194
BmgT120I GGNCC 3 cut(s) 295, 1095, 1194
BmiI GGNNCC 1 cut(s) 1196
BmrFI CCNGG 2 cut(s) 319, 931
BmsI GCATC 2 cut(s) 331, 484
BmtI GCTAGC 1 cut(s) 1065
BpiI GAAGAC 1 cut(s) 794
Bpu14I TTCGAA 1 cut(s) 812
BsaI GGTCTC 1 cut(s) 107
BsaJI CCNNGG 5 cut(s) 173, 201, 929, 930, 1056
Bsc4I CCNNNNNNNGG 3 cut(s) 207, 724, 935
Bse1I ACTGG 2 cut(s) 453, 771
Bse3DI GCAATG 3 cut(s) 802, 858, 1200
BseBI CCWGG 2 cut(s) 319, 931
BseDI CCNNGG 5 cut(s) 173, 201, 929, 930, 1056
BseGI GGATG 2 cut(s) 338, 852
BseLI CCNNNNNNNGG 3 cut(s) 207, 724, 935
BseMI GCAATG 3 cut(s) 802, 858, 1200
BseMII CTCAG 5 cut(s) 151, 180, 594, 818, 1128
BseNI ACTGG 2 cut(s) 453, 771
BseRI GAGGAG 2 cut(s) 144, 704
BseXI GCAGC 8 cut(s) 8, 13, 275, 578, 720, 953, 1068, 1165
BsgI GTGCAG 3 cut(s) 960, 1144, 1164
BshFI GGCC 3 cut(s) 42, 59, 627
BsiHKAI GWGCWC 2 cut(s) 154, 1115
BslFI GGGAC 1 cut(s) 1180
BslI CCNNNNNNNGG 3 cut(s) 207, 724, 935
BsmAI GTCTC 3 cut(s) 107, 185, 826
BsmFI GGGAC 1 cut(s) 1180
BsnI GGCC 3 cut(s) 42, 59, 627
Bso31I GGTCTC 1 cut(s) 107
Bsp119I TTCGAA 1 cut(s) 812
Bsp1286I GDGCHC 2 cut(s) 154, 1115
Bsp143I GATC 6 cut(s) 120, 168, 893, 1117, 1215, 1222
Bsp19I CCATGG 1 cut(s) 173
BspACI CCGC 3 cut(s) 39, 99, 224
BspANI GGCC 3 cut(s) 42, 59, 627
BspCNI CTCAG 5 cut(s) 150, 181, 593, 819, 1127
BspHI TCATGA 1 cut(s) 648
BspLI GGNNCC 1 cut(s) 1196
BspMI ACCTGC 1 cut(s) 44
BspOI GCTAGC 1 cut(s) 1065
BspPI GGATC 3 cut(s) 163, 901, 1210
BspQI GCTCTTC 1 cut(s) 190
BspT104I TTCGAA 1 cut(s) 812
BspTNI GGTCTC 1 cut(s) 107
BsrDI GCAATG 3 cut(s) 802, 858, 1200
BsrI ACTGG 2 cut(s) 453, 771
BssECI CCNNGG 5 cut(s) 173, 201, 929, 930, 1056
BssMI GATC 6 cut(s) 120, 168, 893, 1117, 1215, 1222
BssT1I CCWWGG 3 cut(s) 173, 201, 1056
Bst2UI CCWGG 2 cut(s) 319, 931
Bst4CI ACNGT 2 cut(s) 476, 859
Bst6I CTCTTC 2 cut(s) 110, 190
BstAPI GCANNNNNTGC 3 cut(s) 269, 572, 683
BstBI TTCGAA 1 cut(s) 812
BstC8I GCNNGC 4 cut(s) 90, 436, 793, 1063
BstDEI CTNAG 5 cut(s) 137, 189, 580, 827, 1114
BstDSI CCRYGG 1 cut(s) 173
BstF5I GGATG 2 cut(s) 338, 852
BstHHI GCGC 2 cut(s) 26, 274
BstKTI GATC 6 cut(s) 123, 171, 896, 1120, 1218, 1225
BstMAI GTCTC 3 cut(s) 107, 185, 826
BstMBI GATC 6 cut(s) 120, 168, 893, 1117, 1215, 1222
BstMWI GCNNNNNNNGC 8 cut(s) 18, 48, 89, 269, 572, 683, 792, 887
BstNI CCWGG 2 cut(s) 319, 931
BstNSI RCATGY 1 cut(s) 1069
BstSCI CCNGG 2 cut(s) 317, 929
BstV1I GCAGC 8 cut(s) 8, 13, 275, 578, 720, 953, 1068, 1165
BstV2I GAAGAC 1 cut(s) 794
BstX2I RGATCY 2 cut(s) 168, 1117
BstXI CCANNNNNNTGG 1 cut(s) 349
BstYI RGATCY 2 cut(s) 168, 1117
BsuRI GGCC 3 cut(s) 42, 59, 627
BtgI CCRYGG 1 cut(s) 173
BtsCI GGATG 2 cut(s) 338, 852
BtsI GCAGTG 1 cut(s) 533
BtsIMutI CAGTG 2 cut(s) 533, 778
BveI ACCTGC 1 cut(s) 44
Cac8I GCNNGC 4 cut(s) 90, 436, 793, 1063
CaiI CAGNNNCTG 1 cut(s) 1158
CciI TCATGA 1 cut(s) 648
CfoI GCGC 2 cut(s) 26, 274
Cfr13I GGNCC 3 cut(s) 295, 1095, 1194
Csp6I GTAC 3 cut(s) 477, 831, 874
CviQI GTAC 3 cut(s) 477, 831, 874
DdeI CTNAG 5 cut(s) 137, 189, 580, 827, 1114
DpnI GATC 6 cut(s) 122, 170, 895, 1119, 1217, 1224
DpnII GATC 6 cut(s) 120, 168, 893, 1117, 1215, 1222
Eam1104I CTCTTC 2 cut(s) 110, 190
EarI CTCTTC 2 cut(s) 110, 190
Ecl136II GAGCTC 1 cut(s) 1113
Eco130I CCWWGG 3 cut(s) 173, 201, 1056
Eco24I GRGCYC 1 cut(s) 1115
Eco31I GGTCTC 1 cut(s) 107
Eco32I GATATC 1 cut(s) 732
Eco47I GGWCC 3 cut(s) 295, 1095, 1194
Eco53kI GAGCTC 1 cut(s) 1113
EcoICRI GAGCTC 1 cut(s) 1113
EcoRII CCWGG 2 cut(s) 317, 929
EcoRV GATATC 1 cut(s) 732
EcoT14I CCWWGG 3 cut(s) 173, 201, 1056
EcoT22I ATGCAT 1 cut(s) 499
EcoT38I GRGCYC 1 cut(s) 1115
ErhI CCWWGG 3 cut(s) 173, 201, 1056
FalI AAGNNNNNCTT 2 cut(s) 213, 245
FaqI GGGAC 1 cut(s) 1180
FokI GGATG 2 cut(s) 345, 859
FriOI GRGCYC 1 cut(s) 1115
FspBI CTAG 3 cut(s) 84, 1062, 1234
GlaI GCGC 2 cut(s) 25, 273
HaeIII GGCC 3 cut(s) 42, 59, 627
HhaI GCGC 2 cut(s) 26, 274
Hin6I GCGC 2 cut(s) 24, 272
HinP1I GCGC 2 cut(s) 24, 272
HinfI GANTC 5 cut(s) 133, 193, 314, 658, 1155
HphI GGTGA 6 cut(s) 626, 712, 739, 797, 906, 918
Hpy166II GTNNAC 2 cut(s) 776, 1137
Hpy188I TCNGA 5 cut(s) 140, 190, 1108, 1117, 1154
Hpy188III TCNNGA 2 cut(s) 649, 1226
Hpy8I GTNNAC 2 cut(s) 776, 1137
HpyAV CCTTC 5 cut(s) 618, 691, 845, 893, 977
HpyCH4III ACNGT 2 cut(s) 476, 859
HpyCH4IV ACGT 2 cut(s) 1034, 1128
HpyCH4V TGCA 8 cut(s) 497, 533, 566, 677, 795, 941, 1161, 1181
HpyF10VI GCNNNNNNNGC 8 cut(s) 18, 48, 89, 269, 572, 683, 792, 887
HpyF3I CTNAG 5 cut(s) 137, 189, 580, 827, 1114
HpySE526I ACGT 2 cut(s) 1034, 1128
HspAI GCGC 2 cut(s) 24, 272
Kzo9I GATC 6 cut(s) 120, 168, 893, 1117, 1215, 1222
LguI GCTCTTC 1 cut(s) 190
LmnI GCTCC 2 cut(s) 157, 1210
Lsp1109I GCAGC 8 cut(s) 8, 13, 275, 578, 720, 953, 1068, 1165
LweI GCATC 2 cut(s) 331, 484
MaeI CTAG 3 cut(s) 84, 1062, 1234
MaeII ACGT 2 cut(s) 1034, 1128
MaeIII GTNAC 2 cut(s) 687, 1171
MalI GATC 6 cut(s) 122, 170, 895, 1119, 1217, 1224
MboI GATC 6 cut(s) 120, 168, 893, 1117, 1215, 1222
MfeI CAATTG 1 cut(s) 549
MflI RGATCY 2 cut(s) 168, 1117
MhlI GDGCHC 2 cut(s) 154, 1115
MluCI AATT 4 cut(s) 247, 425, 549, 904
MlyI GAGTC 1 cut(s) 187
MmeI TCCRAC 2 cut(s) 957, 1073
Mph1103I ATGCAT 1 cut(s) 499
MroXI GAANNNNTTC 1 cut(s) 225
MslI CAYNNNNRTG 4 cut(s) 327, 647, 653, 786
MspA1I CMGCKG 1 cut(s) 944
MspR9I CCNGG 2 cut(s) 319, 931
MunI CAATTG 1 cut(s) 549
MvaI CCWGG 2 cut(s) 319, 931
MwoI GCNNNNNNNGC 8 cut(s) 18, 48, 89, 269, 572, 683, 792, 887
NcoI CCATGG 1 cut(s) 173
NdeII GATC 6 cut(s) 120, 168, 893, 1117, 1215, 1222
NheI GCTAGC 1 cut(s) 1061
NlaIV GGNNCC 1 cut(s) 1196
NsiI ATGCAT 1 cut(s) 499
NspI RCATGY 1 cut(s) 1069
NspV TTCGAA 1 cut(s) 812
PagI TCATGA 1 cut(s) 648
PaqCI CACCTGC 1 cut(s) 44
PasI CCCWGGG 1 cut(s) 930
PciSI GCTCTTC 1 cut(s) 190
PdmI GAANNNNTTC 1 cut(s) 225
PfeI GAWTC 4 cut(s) 133, 314, 658, 1155
PflFI GACNNNGTC 1 cut(s) 395
PflMI CCANNNNNTGG 1 cut(s) 207
PleI GAGTC 1 cut(s) 187
PpsI GAGTC 1 cut(s) 187
PsiI TTATAA 1 cut(s) 278
Psp124BI GAGCTC 1 cut(s) 1115
Psp6I CCWGG 2 cut(s) 317, 929
PspGI CCWGG 2 cut(s) 317, 929
PspN4I GGNNCC 1 cut(s) 1196
PspPI GGNCC 3 cut(s) 295, 1095, 1194
PstNI CAGNNNCTG 1 cut(s) 1158
PsuI RGATCY 2 cut(s) 168, 1117
PsyI GACNNNGTC 1 cut(s) 395
PvuII CAGCTG 1 cut(s) 944
RsaI GTAC 3 cut(s) 478, 832, 875
RsaNI GTAC 3 cut(s) 477, 831, 874
RseI CAYNNNNRTG 4 cut(s) 327, 647, 653, 786
SacI GAGCTC 1 cut(s) 1115
SapI GCTCTTC 1 cut(s) 190
Sau3AI GATC 6 cut(s) 120, 168, 893, 1117, 1215, 1222
Sau96I GGNCC 3 cut(s) 295, 1095, 1194
SchI GAGTC 1 cut(s) 187
ScrFI CCNGG 2 cut(s) 319, 931
SduI GDGCHC 2 cut(s) 154, 1115
SfaNI GCATC 2 cut(s) 331, 484
SfuI TTCGAA 1 cut(s) 812
SinI GGWCC 3 cut(s) 295, 1095, 1194
SmiMI CAYNNNNRTG 4 cut(s) 327, 647, 653, 786
Sse9I AATT 4 cut(s) 247, 425, 549, 904
SsiI CCGC 3 cut(s) 39, 99, 224
SspMI CTAG 3 cut(s) 84, 1062, 1234
SstI GAGCTC 1 cut(s) 1115
StyD4I CCNGG 2 cut(s) 317, 929
StyI CCWWGG 3 cut(s) 173, 201, 1056
TaaI ACNGT 2 cut(s) 476, 859
TaiI ACGT 2 cut(s) 1037, 1131
TaqI TCGA 1 cut(s) 812
TasI AATT 4 cut(s) 247, 425, 549, 904
TatI WGTACW 2 cut(s) 476, 873
TauI GCSGC 1 cut(s) 42
TfiI GAWTC 4 cut(s) 133, 314, 658, 1155
TscAI CASTG 2 cut(s) 540, 778
TseI GCWGC 9 cut(s) 9, 21, 26, 263, 566, 708, 941, 1081, 1178
TspDTI ATGAA 3 cut(s) 373, 583, 631
TspRI CASTG 2 cut(s) 540, 778
Tth111I GACNNNGTC 1 cut(s) 395
Van91I CCANNNNNTGG 1 cut(s) 207
VpaK11BI GGWCC 3 cut(s) 295, 1095, 1194
XapI RAATTY 3 cut(s) 247, 425, 904
XceI RCATGY 1 cut(s) 1069
XmnI GAANNNNTTC 1 cut(s) 225
XspI CTAG 3 cut(s) 84, 1062, 1234
Zsp2I ATGCAT 1 cut(s) 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.