MD13G1183100.v1.1

TVP38 TMEM64 family membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0010842
Physical Location & Seq
Forward (+)
15416587 .. 15421545
4959 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1183100.v1.1.491

Sequence Viewer

Length: 825 bp
ATGGCGTTCACTTGGGGTTCCGCGCTCAGGATCGCTCTCCTCCTCCTCCTCCTGGCCGCCGTTGCCACTGCTTGTTTCACTCTTCCCGTGGAAAAGATTTTGAAGGACTTCTTATTATGGGTTGAGCAGGATCTTGGTCCCTGGGGTCCCCTTGTGCTGGCTGTTGCATACATTCCACTAACAGTCTTGGCAGTACCAGCTTCAGTACTTACTCTCGGTGGTGGTTATCTTTTTGGGCTACCTGTTGGCTTTGTTGCTGATTCAATTGGAGCCACTTTAGGTGCAGGGGCTGCTTTTCTTCTAGGAAGAACTATTGGGAGATCATTTGTTGTTTCAAGGTTGAAGGATTATCCTCAGTTCCGGGCAGTTGCAATTGCAATTCGGAGATCCGGATTTAAGATTGTTTTGTTGCTTCGCCTTGTTCCCTTGCTCCCATTCAACATGCTAAATTACCTCCTTTCTGTGACTCCTGTTCCAATAGGACAATACATGCTGGCTTCCTGGATAGGGATGATGCCAATAACATTTGCGCTAGTATATGTTGGAACAACTCTCAAGGATCTTTCTGATGTGACCCATGGATGGGGTGAATTTTCAAAGACCCGTTGGGCTTTTATCATATTGGGCCTTGTAGTCTCTGTGATTTTAATGATTTGTGTTACAAGAGTTGCCAAGGCCGCTTTGGAAAAAGCTTTGGCCGAAAATGAGGATATTGATAACATTGATATTATACCAGAGTTACCTGTTGTCTCCGAAACACCAGTGGATCTCGACCAGCCTCTTTTAATCAAGATAGACGCGTCTGACGACCATCATGAGAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

275

Amino Acids

29.71

Weight (kDa)

5.62

Isoelectric Point (pI)

34.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 65 - 183 1.9e-24 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010842)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19070 AT5G19070
fragaria_vesca FvH4_4g14490 FvH4_4g14490 FvH4_4g14490 FvH4_4g14490
malus_domestica MD13G1183100.v1.1 MD16G1184000.v1.1
prunus_persica Prupe.1G151400_v2.0.a1
pyrus_communis pycom13g15830 pycom16g15450
rosa_chinensis RchiOBHm_Chr4g0418341
rosa_laevigata RLG00000007873 RLG00000007874
rosa_multiflora Rmu_sc0009909.1_g000002
rosa_roxburghii Rroxscaffold_5G00361530
rosa_rugosa Rorug04G0152300
rosa_samantha Rh4AG213900 Rh4BG216500
rosa_wichuraiana Rw4G018170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 23, 800
AccIII TCCGGA 1 cut(s) 389
AciI CCGC 3 cut(s) 21, 57, 678
AclWI GGATC 5 cut(s) 38, 138, 381, 567, 774
AcoI YGGCCR 2 cut(s) 54, 696
AcsI RAATTY 1 cut(s) 590
AcuI CTGAAG 1 cut(s) 186
AfaI GTAC 2 cut(s) 195, 207
AfiI CCNNNNNNNGG 6 cut(s) 27, 52, 157, 507, 582, 583
AflIII ACRYGT 1 cut(s) 798
AgsI TTSAA 6 cut(s) 103, 264, 336, 343, 439, 597
AjnI CCWGG 3 cut(s) 51, 140, 500
AluBI AGCT 2 cut(s) 200, 692
AluI AGCT 2 cut(s) 200, 692
Alw26I GTCTC 2 cut(s) 640, 754
AlwI GGATC 5 cut(s) 38, 138, 381, 567, 774
AlwNI CAGNNNCTG 1 cut(s) 290
Aor13HI TCCGGA 1 cut(s) 389
AoxI GGCC 4 cut(s) 54, 625, 675, 696
ApeKI GCWGC 1 cut(s) 290
ApoI RAATTY 1 cut(s) 590
Asp700I GAANNNNTTC 1 cut(s) 107
AspLEI GCGC 2 cut(s) 25, 532
AspS9I GGNCC 3 cut(s) 137, 146, 625
AsuC2I CCSGG 1 cut(s) 362
AsuHPI GGTGA 1 cut(s) 599
AvaII GGWCC 2 cut(s) 137, 146
BbvI GCAGC 1 cut(s) 277
BccI CCATC 2 cut(s) 576, 819
BceAI ACGGC 1 cut(s) 44
BciT130I CCWGG 3 cut(s) 53, 142, 502
BcnI CCSGG 1 cut(s) 362
BcoDI GTCTC 2 cut(s) 640, 754
BfaI CTAG 2 cut(s) 302, 533
BisI GCNGC 3 cut(s) 57, 291, 678
BlsI GCNGC 3 cut(s) 58, 292, 679
BmcAI AGTACT 1 cut(s) 207
Bme1390I CCNGG 4 cut(s) 53, 142, 362, 502
Bme18I GGWCC 2 cut(s) 137, 146
BmgT120I GGNCC 3 cut(s) 137, 146, 625
BmiI GGNNCC 5 cut(s) 19, 139, 147, 148, 271
BmrFI CCNGG 4 cut(s) 53, 142, 362, 502
BmsI GCATC 1 cut(s) 504
Bpu10I CCTNAGC 1 cut(s) 26
BpuEI CTTGAG 1 cut(s) 539
BpuMI CCSGG 1 cut(s) 362
BsaJI CCNNGG 5 cut(s) 87, 140, 141, 577, 672
BsaWI WCCGGW 1 cut(s) 389
Bsc4I CCNNNNNNNGG 6 cut(s) 27, 52, 157, 507, 582, 583
Bse1I ACTGG 1 cut(s) 761
BseAI TCCGGA 1 cut(s) 389
BseBI CCWGG 3 cut(s) 53, 142, 502
BseDI CCNNGG 5 cut(s) 87, 140, 141, 577, 672
BseGI GGATG 2 cut(s) 516, 587
BseLI CCNNNNNNNGG 6 cut(s) 27, 52, 157, 507, 582, 583
BseMII CTCAG 2 cut(s) 40, 368
BseNI ACTGG 1 cut(s) 761
BseRI GAGGAG 4 cut(s) 29, 32, 35, 38
BseXI GCAGC 1 cut(s) 277
BsgI GTGCAG 1 cut(s) 303
Bsh1236I CGCG 2 cut(s) 23, 800
BshFI GGCC 4 cut(s) 56, 627, 677, 698
BsiSI CCGG 2 cut(s) 361, 390
BslFI GGGAC 2 cut(s) 123, 132
BslI CCNNNNNNNGG 6 cut(s) 27, 52, 157, 507, 582, 583
BsmAI GTCTC 2 cut(s) 640, 754
BsmFI GGGAC 2 cut(s) 123, 132
BsnI GGCC 4 cut(s) 56, 627, 677, 698
Bsp13I TCCGGA 1 cut(s) 389
Bsp143I GATC 6 cut(s) 30, 130, 320, 386, 559, 766
Bsp19I CCATGG 1 cut(s) 577
BspACI CCGC 3 cut(s) 21, 57, 678
BspANI GGCC 4 cut(s) 56, 627, 677, 698
BspCNI CTCAG 2 cut(s) 39, 367
BspEI TCCGGA 1 cut(s) 389
BspFNI CGCG 2 cut(s) 23, 800
BspHI TCATGA 1 cut(s) 814
BspLI GGNNCC 5 cut(s) 19, 139, 147, 148, 271
BspPI GGATC 5 cut(s) 38, 138, 381, 567, 774
BsrI ACTGG 1 cut(s) 761
BssECI CCNNGG 5 cut(s) 87, 140, 141, 577, 672
BssMI GATC 6 cut(s) 30, 130, 320, 386, 559, 766
BssT1I CCWWGG 2 cut(s) 577, 672
Bst2UI CCWGG 3 cut(s) 53, 142, 502
Bst4CI ACNGT 1 cut(s) 184
Bst6I CTCTTC 1 cut(s) 87
BstAPI GCANNNNNTGC 1 cut(s) 290
BstC8I GCNNGC 2 cut(s) 159, 495
BstDEI CTNAG 2 cut(s) 26, 354
BstDSI CCRYGG 2 cut(s) 87, 577
BstF5I GGATG 2 cut(s) 516, 587
BstFNI CGCG 2 cut(s) 23, 800
BstHHI GCGC 2 cut(s) 25, 532
BstKTI GATC 6 cut(s) 33, 133, 323, 389, 562, 769
BstMAI GTCTC 2 cut(s) 640, 754
BstMBI GATC 6 cut(s) 30, 130, 320, 386, 559, 766
BstMWI GCNNNNNNNGC 4 cut(s) 62, 197, 290, 677
BstNI CCWGG 3 cut(s) 53, 142, 502
BstNSI RCATGY 2 cut(s) 445, 493
BstSCI CCNGG 4 cut(s) 51, 140, 360, 500
BstUI CGCG 2 cut(s) 23, 800
BstV1I GCAGC 1 cut(s) 277
BstX2I RGATCY 4 cut(s) 130, 386, 559, 766
BstYI RGATCY 4 cut(s) 130, 386, 559, 766
BsuRI GGCC 4 cut(s) 56, 627, 677, 698
BtgI CCRYGG 2 cut(s) 87, 577
BtsCI GGATG 2 cut(s) 516, 587
BtsI GCAGTG 1 cut(s) 66
BtsIMutI CAGTG 2 cut(s) 66, 768
Cac8I GCNNGC 2 cut(s) 159, 495
CaiI CAGNNNCTG 1 cut(s) 290
CciI TCATGA 1 cut(s) 814
CfoI GCGC 2 cut(s) 25, 532
Cfr13I GGNCC 3 cut(s) 137, 146, 625
CseI GACGC 2 cut(s) 789, 806
Csp6I GTAC 2 cut(s) 194, 206
CviAII CATG 4 cut(s) 442, 490, 578, 815
CviQI GTAC 2 cut(s) 194, 206
DdeI CTNAG 2 cut(s) 26, 354
DpnI GATC 6 cut(s) 32, 132, 322, 388, 561, 768
DpnII GATC 6 cut(s) 30, 130, 320, 386, 559, 766
EaeI YGGCCR 2 cut(s) 54, 696
Eam1104I CTCTTC 1 cut(s) 87
EarI CTCTTC 1 cut(s) 87
Eco130I CCWWGG 2 cut(s) 577, 672
Eco47I GGWCC 2 cut(s) 137, 146
Eco57I CTGAAG 1 cut(s) 186
EcoO109I RGGNCCY 1 cut(s) 146
EcoRII CCWGG 3 cut(s) 51, 140, 500
EcoT14I CCWWGG 2 cut(s) 577, 672
ErhI CCWWGG 2 cut(s) 577, 672
FaeI CATG 4 cut(s) 445, 493, 581, 818
FalI AAGNNNNNCTT 2 cut(s) 95, 127
FaqI GGGAC 2 cut(s) 123, 132
FatI CATG 4 cut(s) 441, 489, 577, 814
Fnu4HI GCNGC 3 cut(s) 57, 291, 678
FokI GGATG 2 cut(s) 523, 594
Fsp4HI GCNGC 3 cut(s) 57, 291, 678
FspBI CTAG 2 cut(s) 302, 533
GlaI GCGC 2 cut(s) 24, 531
GluI GCNGC 3 cut(s) 57, 291, 678
HaeIII GGCC 4 cut(s) 56, 627, 677, 698
HapII CCGG 2 cut(s) 361, 390
HgaI GACGC 2 cut(s) 789, 806
HhaI GCGC 2 cut(s) 25, 532
Hin1II CATG 4 cut(s) 445, 493, 581, 818
Hin6I GCGC 2 cut(s) 23, 530
HinP1I GCGC 2 cut(s) 23, 530
HindIII AAGCTT 1 cut(s) 690
HinfI GANTC 2 cut(s) 260, 466
HpaII CCGG 2 cut(s) 361, 390
HphI GGTGA 1 cut(s) 599
Hpy166II GTNNAC 1 cut(s) 9
Hpy188I TCNGA 4 cut(s) 384, 568, 754, 805
Hpy188III TCNNGA 5 cut(s) 28, 390, 770, 790, 815
Hpy8I GTNNAC 1 cut(s) 9
HpyAV CCTTC 2 cut(s) 97, 337
HpyCH4III ACNGT 1 cut(s) 184
HpyCH4V TGCA 4 cut(s) 167, 284, 371, 377
HpyF10VI GCNNNNNNNGC 4 cut(s) 62, 197, 290, 677
HpyF3I CTNAG 2 cut(s) 26, 354
Hsp92II CATG 4 cut(s) 445, 493, 581, 818
HspAI GCGC 2 cut(s) 23, 530
KflI GGGWCCC 1 cut(s) 146
Kpn2I TCCGGA 1 cut(s) 389
Kzo9I GATC 6 cut(s) 30, 130, 320, 386, 559, 766
LmnI GCTCC 2 cut(s) 269, 435
Lsp1109I GCAGC 1 cut(s) 277
LweI GCATC 1 cut(s) 504
MaeI CTAG 2 cut(s) 302, 533
MaeIII GTNAC 4 cut(s) 463, 571, 658, 738
MalI GATC 6 cut(s) 32, 132, 322, 388, 561, 768
MboI GATC 6 cut(s) 30, 130, 320, 386, 559, 766
MboII GAAGA 3 cut(s) 74, 290, 318
MfeI CAATTG 2 cut(s) 264, 372
MflI RGATCY 4 cut(s) 130, 386, 559, 766
MluCI AATT 5 cut(s) 264, 372, 378, 448, 590
MluI ACGCGT 1 cut(s) 798
MlyI GAGTC 1 cut(s) 460
MmeI TCCRAC 1 cut(s) 523
MnlI CCTC 8 cut(s) 50, 53, 56, 59, 363, 464, 700, 789
MroI TCCGGA 1 cut(s) 389
MroXI GAANNNNTTC 1 cut(s) 107
MseI TTAA 3 cut(s) 396, 647, 785
MspI CCGG 2 cut(s) 361, 390
MspR9I CCNGG 4 cut(s) 53, 142, 362, 502
MunI CAATTG 2 cut(s) 264, 372
MvaI CCWGG 3 cut(s) 53, 142, 502
MvnI CGCG 2 cut(s) 23, 800
MwoI GCNNNNNNNGC 4 cut(s) 62, 197, 290, 677
NciI CCSGG 1 cut(s) 362
NcoI CCATGG 1 cut(s) 577
NdeII GATC 6 cut(s) 30, 130, 320, 386, 559, 766
NlaIII CATG 4 cut(s) 445, 493, 581, 818
NlaIV GGNNCC 5 cut(s) 19, 139, 147, 148, 271
NmuCI GTSAC 2 cut(s) 463, 571
NspI RCATGY 2 cut(s) 445, 493
PagI TCATGA 1 cut(s) 814
PasI CCCWGGG 1 cut(s) 141
PcsI WCGNNNNNNNCGW 1 cut(s) 804
PdmI GAANNNNTTC 1 cut(s) 107
PfeI GAWTC 1 cut(s) 260
PfoI TCCNGGA 1 cut(s) 500
PkrI GCNGC 3 cut(s) 58, 292, 679
PleI GAGTC 1 cut(s) 460
PpsI GAGTC 1 cut(s) 460
PpuMI RGGWCCY 1 cut(s) 146
Psp5II RGGWCCY 1 cut(s) 146
Psp6I CCWGG 3 cut(s) 51, 140, 500
PspGI CCWGG 3 cut(s) 51, 140, 500
PspN4I GGNNCC 5 cut(s) 19, 139, 147, 148, 271
PspPI GGNCC 3 cut(s) 137, 146, 625
PspPPI RGGWCCY 1 cut(s) 146
PstNI CAGNNNCTG 1 cut(s) 290
PsuI RGATCY 4 cut(s) 130, 386, 559, 766
RsaI GTAC 2 cut(s) 195, 207
RsaNI GTAC 2 cut(s) 194, 206
SaqAI TTAA 3 cut(s) 396, 647, 785
SatI GCNGC 3 cut(s) 57, 291, 678
Sau3AI GATC 6 cut(s) 30, 130, 320, 386, 559, 766
Sau96I GGNCC 3 cut(s) 137, 146, 625
ScaI AGTACT 1 cut(s) 207
SchI GAGTC 1 cut(s) 460
ScrFI CCNGG 4 cut(s) 53, 142, 362, 502
SetI ASST 7 cut(s) 202, 244, 283, 341, 456, 694, 745
SfaNI GCATC 1 cut(s) 504
SinI GGWCC 2 cut(s) 137, 146
SmlI CTYRAG 1 cut(s) 554
SmoI CTYRAG 1 cut(s) 554
Sse9I AATT 5 cut(s) 264, 372, 378, 448, 590
SsiI CCGC 3 cut(s) 21, 57, 678
SspMI CTAG 2 cut(s) 302, 533
StyD4I CCNGG 4 cut(s) 51, 140, 360, 500
StyI CCWWGG 2 cut(s) 577, 672
TaaI ACNGT 1 cut(s) 184
TaqI TCGA 1 cut(s) 771
TasI AATT 5 cut(s) 264, 372, 378, 448, 590
TatI WGTACW 1 cut(s) 205
TauI GCSGC 2 cut(s) 59, 680
TfiI GAWTC 1 cut(s) 260
Tru1I TTAA 3 cut(s) 396, 647, 785
Tru9I TTAA 3 cut(s) 396, 647, 785
TscAI CASTG 2 cut(s) 73, 768
TseFI GTSAC 2 cut(s) 463, 571
TseI GCWGC 1 cut(s) 290
Tsp45I GTSAC 2 cut(s) 463, 571
TspRI CASTG 2 cut(s) 73, 768
VpaK11BI GGWCC 2 cut(s) 137, 146
XapI RAATTY 1 cut(s) 590
XceI RCATGY 2 cut(s) 445, 493
XcmI CCANNNNNNNNNTGG 1 cut(s) 679
XmnI GAANNNNTTC 1 cut(s) 107
XspI CTAG 2 cut(s) 302, 533
ZrmI AGTACT 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.