RLG00000007874

TVP38 TMEM64 family membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0010842
Physical Location & Seq
Forward (+)
22311137 .. 22315329
4193 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007874

Sequence Viewer

Length: 735 bp
ATGCTAAGGGGTCCCAATTGTGAGATTATTTGTGTTCCGATAATCTGGATAGTTGTGCACGATGACTTCCATTCACGTTCTAAGTTAAACCAGTTCCTAAAATCATTGGCCGTTGCATACATTCCTTTAACGGTCTTGGCAGTAGCAGCTTCAGTACTTACGCTTGGTGGTGGTTATCTTTTTGGCCTGCCTGTAGGCTTTGTTGCCGACTCAATTGGTGCCTCTATAGGTGCAGGGGCTGCATTCCTTCTTGGCAGAACTATTGGGAGATCATTCATCATTTCCAAGTTGAAGGATTATCCTCAGTTTCGTGCAATTGTTTTGCTGCTTCGTCTTGTTCCCTTGCTTCCATTCAACATGTTAAATTACCTCCTGTCGGTGACTCCTGTTCCAATTGGAGAATACATGCTTGCTTCATGGTTAGGAATGACGCCGATAACACTTGCACTAGTATATGTTGGAACAACTCTCAAGGATCTTTCTGATGTGACCCATGGTTGGGGTGAATTTTCAAAGACCTGTTGGGCTGTCATCATACTGAGCCTTGTAGTATCCGTGATTCTAATGTTTCTTGTTACAAGAGTTACCAAGTCTGCTTTGGAAAAAGCTTTGGCTGAAAATGAGGATCTTGATATCATAACGCCACCTGAGTTACCTGTTTCTGATACACCTGGTCATCTCGACCAGCCGCTTTTAATCAAGATAGATCCCTCTGAAGACCATCATGAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

245

Amino Acids

26.57

Weight (kDa)

5.83

Isoelectric Point (pI)

25.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 51 - 155 1.3e-16 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010842)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19070 AT5G19070
fragaria_vesca FvH4_4g14490 FvH4_4g14490 FvH4_4g14490 FvH4_4g14490
malus_domestica MD13G1183100.v1.1 MD16G1184000.v1.1
prunus_persica Prupe.1G151400_v2.0.a1
pyrus_communis pycom13g15830 pycom16g15450
rosa_chinensis RchiOBHm_Chr4g0418341
rosa_laevigata RLG00000007873 RLG00000007874
rosa_multiflora Rmu_sc0009909.1_g000002
rosa_roxburghii Rroxscaffold_5G00361530
rosa_rugosa Rorug04G0152300
rosa_samantha Rh4AG213900 Rh4BG216500
rosa_wichuraiana Rw4G018170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 218
AciI CCGC 1 cut(s) 689
AclWI GGATC 3 cut(s) 483, 633, 701
AcoI YGGCCR 1 cut(s) 108
AcsI RAATTY 1 cut(s) 506
AcuI CTGAAG 2 cut(s) 135, 735
AcyI GRCGYC 1 cut(s) 431
AfaI GTAC 1 cut(s) 156
AfiI CCNNNNNNNGG 3 cut(s) 376, 498, 499
AflIII ACRYGT 1 cut(s) 357
AgsI TTSAA 3 cut(s) 292, 355, 513
AhlI ACTAGT 1 cut(s) 448
AjnI CCWGG 1 cut(s) 670
AluBI AGCT 2 cut(s) 149, 608
AluI AGCT 2 cut(s) 149, 608
Alw21I GWGCWC 1 cut(s) 60
Alw44I GTGCAC 1 cut(s) 56
AlwI GGATC 3 cut(s) 483, 633, 701
AlwNI CAGNNNCTG 1 cut(s) 239
AoxI GGCC 2 cut(s) 108, 184
ApaLI GTGCAC 1 cut(s) 56
ApeKI GCWGC 3 cut(s) 146, 239, 325
ApoI RAATTY 1 cut(s) 506
AspS9I GGNCC 1 cut(s) 11
AsuHPI GGTGA 2 cut(s) 391, 515
AvaII GGWCC 1 cut(s) 11
BaeGI GKGCMC 1 cut(s) 60
BanI GGYRCC 1 cut(s) 218
BbsI GAAGAC 1 cut(s) 723
Bbv12I GWGCWC 1 cut(s) 60
BbvI GCAGC 3 cut(s) 158, 226, 312
BccI CCATC 1 cut(s) 729
BceAI ACGGC 1 cut(s) 95
BciT130I CCWGG 1 cut(s) 672
BciVI GTATCC 1 cut(s) 562
BcuI ACTAGT 1 cut(s) 448
BfaI CTAG 1 cut(s) 449
BfmI CTRYAG 2 cut(s) 192, 225
BfuI GTATCC 1 cut(s) 562
BisI GCNGC 4 cut(s) 147, 240, 326, 689
BlsI GCNGC 4 cut(s) 148, 241, 327, 690
BmcAI AGTACT 1 cut(s) 156
Bme1390I CCNGG 1 cut(s) 672
Bme18I GGWCC 1 cut(s) 11
BmgT120I GGNCC 1 cut(s) 11
BmiI GGNNCC 3 cut(s) 12, 13, 220
BmrFI CCNGG 1 cut(s) 672
BpiI GAAGAC 1 cut(s) 723
Bpu10I CCTNAGC 1 cut(s) 5
BpuEI CTTGAG 1 cut(s) 455
BsaHI GRCGYC 1 cut(s) 431
BsaJI CCNNGG 1 cut(s) 493
Bsc4I CCNNNNNNNGG 3 cut(s) 376, 498, 499
Bse1I ACTGG 1 cut(s) 91
BseBI CCWGG 1 cut(s) 672
BseDI CCNNGG 1 cut(s) 493
BseLI CCNNNNNNNGG 3 cut(s) 376, 498, 499
BseMII CTCAG 3 cut(s) 317, 530, 639
BseNI ACTGG 1 cut(s) 91
BseSI GKGCMC 1 cut(s) 60
BseXI GCAGC 3 cut(s) 158, 226, 312
BsgI GTGCAG 1 cut(s) 252
BshFI GGCC 2 cut(s) 110, 186
BshNI GGYRCC 1 cut(s) 218
BsiHKAI GWGCWC 1 cut(s) 60
BslI CCNNNNNNNGG 3 cut(s) 376, 498, 499
BsmI GAATGC 1 cut(s) 242
BsnI GGCC 2 cut(s) 110, 186
Bsp1286I GDGCHC 1 cut(s) 60
Bsp143I GATC 4 cut(s) 269, 475, 625, 706
Bsp19I CCATGG 1 cut(s) 493
BspACI CCGC 1 cut(s) 689
BspANI GGCC 2 cut(s) 110, 186
BspCNI CTCAG 3 cut(s) 316, 531, 640
BspHI TCATGA 1 cut(s) 724
BspLI GGNNCC 3 cut(s) 12, 13, 220
BspPI GGATC 3 cut(s) 483, 633, 701
BspT107I GGYRCC 1 cut(s) 218
BsrI ACTGG 1 cut(s) 91
BssECI CCNNGG 1 cut(s) 493
BssMI GATC 4 cut(s) 269, 475, 625, 706
BssNI GRCGYC 1 cut(s) 431
BssT1I CCWWGG 1 cut(s) 493
Bst2UI CCWGG 1 cut(s) 672
Bst4CI ACNGT 1 cut(s) 133
BstACI GRCGYC 1 cut(s) 431
BstAPI GCANNNNNTGC 1 cut(s) 239
BstC8I GCNNGC 2 cut(s) 188, 411
BstDEI CTNAG 5 cut(s) 5, 81, 303, 539, 648
BstDSI CCRYGG 1 cut(s) 493
BstKTI GATC 4 cut(s) 272, 478, 628, 709
BstMBI GATC 4 cut(s) 269, 475, 625, 706
BstMWI GCNNNNNNNGC 2 cut(s) 146, 239
BstNI CCWGG 1 cut(s) 672
BstNSI RCATGY 2 cut(s) 361, 409
BstSCI CCNGG 1 cut(s) 670
BstSFI CTRYAG 2 cut(s) 192, 225
BstSLI GKGCMC 1 cut(s) 60
BstV1I GCAGC 3 cut(s) 158, 226, 312
BstV2I GAAGAC 1 cut(s) 723
BstX2I RGATCY 3 cut(s) 475, 625, 706
BstYI RGATCY 3 cut(s) 475, 625, 706
BsuI GTATCC 1 cut(s) 562
BsuRI GGCC 2 cut(s) 110, 186
BtgI CCRYGG 1 cut(s) 493
Cac8I GCNNGC 2 cut(s) 188, 411
CaiI CAGNNNCTG 1 cut(s) 239
CciI TCATGA 1 cut(s) 724
Cfr13I GGNCC 1 cut(s) 11
CseI GACGC 1 cut(s) 439
CsiI ACCWGGT 1 cut(s) 670
Csp6I GTAC 1 cut(s) 155
CviAII CATG 5 cut(s) 358, 406, 417, 494, 725
CviQI GTAC 1 cut(s) 155
DdeI CTNAG 5 cut(s) 5, 81, 303, 539, 648
DpnI GATC 4 cut(s) 271, 477, 627, 708
DpnII GATC 4 cut(s) 269, 475, 625, 706
EaeI YGGCCR 1 cut(s) 108
Eco130I CCWWGG 1 cut(s) 493
Eco32I GATATC 1 cut(s) 634
Eco47I GGWCC 1 cut(s) 11
Eco57I CTGAAG 2 cut(s) 135, 735
EcoO109I RGGNCCY 1 cut(s) 11
EcoRII CCWGG 1 cut(s) 670
EcoRV GATATC 1 cut(s) 634
EcoT14I CCWWGG 1 cut(s) 493
ErhI CCWWGG 1 cut(s) 493
FaeI CATG 5 cut(s) 361, 409, 420, 497, 728
FatI CATG 5 cut(s) 357, 405, 416, 493, 724
Fnu4HI GCNGC 4 cut(s) 147, 240, 326, 689
Fsp4HI GCNGC 4 cut(s) 147, 240, 326, 689
FspBI CTAG 1 cut(s) 449
GluI GCNGC 4 cut(s) 147, 240, 326, 689
HaeIII GGCC 2 cut(s) 110, 186
HgaI GACGC 1 cut(s) 439
Hin1I GRCGYC 1 cut(s) 431
Hin1II CATG 5 cut(s) 361, 409, 420, 497, 728
HindIII AAGCTT 1 cut(s) 606
HinfI GANTC 3 cut(s) 209, 382, 559
HphI GGTGA 2 cut(s) 391, 515
Hpy166II GTNNAC 1 cut(s) 58
Hpy188I TCNGA 4 cut(s) 39, 484, 664, 715
Hpy188III TCNNGA 5 cut(s) 46, 629, 680, 700, 725
Hpy8I GTNNAC 1 cut(s) 58
HpyAV CCTTC 2 cut(s) 257, 286
HpyCH4III ACNGT 1 cut(s) 133
HpyCH4IV ACGT 1 cut(s) 76
HpyCH4V TGCA 6 cut(s) 58, 116, 233, 242, 314, 446
HpyF10VI GCNNNNNNNGC 2 cut(s) 146, 239
HpyF3I CTNAG 5 cut(s) 5, 81, 303, 539, 648
HpySE526I ACGT 1 cut(s) 76
Hsp92I GRCGYC 1 cut(s) 431
Hsp92II CATG 5 cut(s) 361, 409, 420, 497, 728
KflI GGGWCCC 1 cut(s) 11
Kzo9I GATC 4 cut(s) 269, 475, 625, 706
Lsp1109I GCAGC 3 cut(s) 158, 226, 312
MabI ACCWGGT 1 cut(s) 670
MaeI CTAG 1 cut(s) 449
MaeII ACGT 1 cut(s) 76
MaeIII GTNAC 5 cut(s) 379, 487, 574, 583, 651
MalI GATC 4 cut(s) 271, 477, 627, 708
MboI GATC 4 cut(s) 269, 475, 625, 706
MboII GAAGA 1 cut(s) 728
MfeI CAATTG 4 cut(s) 16, 213, 315, 393
MflI RGATCY 3 cut(s) 475, 625, 706
MhlI GDGCHC 1 cut(s) 60
MluCI AATT 6 cut(s) 16, 213, 315, 364, 393, 506
MlyI GAGTC 2 cut(s) 203, 376
MmeI TCCRAC 1 cut(s) 439
MnlI CCTC 5 cut(s) 232, 312, 380, 616, 721
MseI TTAA 4 cut(s) 86, 128, 362, 695
MspR9I CCNGG 1 cut(s) 672
MunI CAATTG 4 cut(s) 16, 213, 315, 393
Mva1269I GAATGC 1 cut(s) 242
MvaI CCWGG 1 cut(s) 672
MwoI GCNNNNNNNGC 2 cut(s) 146, 239
NcoI CCATGG 1 cut(s) 493
NdeII GATC 4 cut(s) 269, 475, 625, 706
NlaIII CATG 5 cut(s) 361, 409, 420, 497, 728
NlaIV GGNNCC 3 cut(s) 12, 13, 220
NmuCI GTSAC 2 cut(s) 379, 487
NspI RCATGY 2 cut(s) 361, 409
PagI TCATGA 1 cut(s) 724
PciI ACATGT 1 cut(s) 357
PctI GAATGC 1 cut(s) 242
PfeI GAWTC 1 cut(s) 559
PkrI GCNGC 4 cut(s) 148, 241, 327, 690
PleI GAGTC 2 cut(s) 203, 376
PpsI GAGTC 2 cut(s) 203, 376
PpuMI RGGWCCY 1 cut(s) 11
PscI ACATGT 1 cut(s) 357
Psp5II RGGWCCY 1 cut(s) 11
Psp6I CCWGG 1 cut(s) 670
PspGI CCWGG 1 cut(s) 670
PspN4I GGNNCC 3 cut(s) 12, 13, 220
PspPI GGNCC 1 cut(s) 11
PspPPI RGGWCCY 1 cut(s) 11
PstNI CAGNNNCTG 1 cut(s) 239
PsuI RGATCY 3 cut(s) 475, 625, 706
RsaI GTAC 1 cut(s) 156
RsaNI GTAC 1 cut(s) 155
SaqAI TTAA 4 cut(s) 86, 128, 362, 695
SatI GCNGC 4 cut(s) 147, 240, 326, 689
Sau3AI GATC 4 cut(s) 269, 475, 625, 706
Sau96I GGNCC 1 cut(s) 11
ScaI AGTACT 1 cut(s) 156
SchI GAGTC 2 cut(s) 203, 376
ScrFI CCNGG 1 cut(s) 672
SduI GDGCHC 1 cut(s) 60
SetI ASST 9 cut(s) 79, 151, 232, 372, 521, 610, 649, 658, 673
SexAI ACCWGGT 1 cut(s) 670
SfcI CTRYAG 2 cut(s) 192, 225
SinI GGWCC 1 cut(s) 11
SmlI CTYRAG 1 cut(s) 470
SmoI CTYRAG 1 cut(s) 470
SpeI ACTAGT 1 cut(s) 448
Sse9I AATT 6 cut(s) 16, 213, 315, 364, 393, 506
SsiI CCGC 1 cut(s) 689
SspMI CTAG 1 cut(s) 449
StyD4I CCNGG 1 cut(s) 670
StyI CCWWGG 1 cut(s) 493
TaaI ACNGT 1 cut(s) 133
TaiI ACGT 1 cut(s) 79
TaqI TCGA 1 cut(s) 681
TasI AATT 6 cut(s) 16, 213, 315, 364, 393, 506
TatI WGTACW 1 cut(s) 154
TauI GCSGC 1 cut(s) 691
TfiI GAWTC 1 cut(s) 559
Tru1I TTAA 4 cut(s) 86, 128, 362, 695
Tru9I TTAA 4 cut(s) 86, 128, 362, 695
TseFI GTSAC 2 cut(s) 379, 487
TseI GCWGC 3 cut(s) 146, 239, 325
Tsp45I GTSAC 2 cut(s) 379, 487
TspDTI ATGAA 2 cut(s) 265, 405
TspGWI ACGGA 1 cut(s) 544
VneI GTGCAC 1 cut(s) 56
VpaK11BI GGWCC 1 cut(s) 11
XapI RAATTY 1 cut(s) 506
XceI RCATGY 2 cut(s) 361, 409
XcmI CCANNNNNNNNNTGG 1 cut(s) 595
XspI CTAG 1 cut(s) 449
ZrmI AGTACT 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.