Rroxscaffold_5G00361530

TVP38 TMEM64 family membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0010842
Physical Location & Seq
Reverse (-)
42224862 .. 42229707
4846 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00361530.1

Sequence Viewer

Length: 819 bp
ATGGCTCTCACATGGAGCTCCGCGCTGAGGATCACACTCCTTCTCCTCCTCCTCGCCGCTGTTGCCACCGCCTGTTTCACTCTCCCCGTTGAAAAGATTTTGAAGGACTTCTTATTATGGGTTGATAAGGATCTTGGCCCGTGGGGTCCATTTGTGCTGGCCGTTGCATACATTCCTTTAACGGTCTTGGCAGTACCAGCTTCAGTACTTACCCTCGGTGGTGGTTATCTTTTTGGCCTGCCTGTCGGCTTTGTTGCCGACTCAATTGGTGCCACTATAGGTGCAGGGGCTGCATTCCTTCTTGGCAGAACTATTGGGAGATCATTCGTCATTTCCAAGTTGAAGGATTATCCTCAGTTTCGTGCAGTTGCGATTGCAATCCGAAAATCTGGATTTAAGATTGTTTTGCTGCTTCGTCTTGTTCCCTTACTTCCATTCAACATGTTAAATTACCTCCTGTCGGTGACTCCTGTTCCAATTGGAGAATACATGCTTGCTTCATGGTTAGGAATGATGCCGATAACACTTGCACTAGTATATGTTGGAACAACTCTCAAGGATCTTTCTGATGTGACCCATGGTTGGGGTGAATTTTCGAAGACCCGTTGGGCTGTCATCATACTGGGCCTTGTAGTATCCGTGATTCTAATGTTTCTTGTTACAAGAGTTGCCAAGGCTGCTTTGGAAAAAGCTTTGGCTGAAAATGAGGATCTTGATATCATAACGCCACCTGAGTTACCTGTTTCTGATACACCTGGTAATCTCGACCAGCCATTTTTAATCAAGATAGATCCCTCTGAAGACCATCATGAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

272

Amino Acids

29.45

Weight (kDa)

6.3

Isoelectric Point (pI)

30.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 65 - 183 2e-24 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010842)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19070 AT5G19070
fragaria_vesca FvH4_4g14490 FvH4_4g14490 FvH4_4g14490 FvH4_4g14490
malus_domestica MD13G1183100.v1.1 MD16G1184000.v1.1
prunus_persica Prupe.1G151400_v2.0.a1
pyrus_communis pycom13g15830 pycom16g15450
rosa_chinensis RchiOBHm_Chr4g0418341
rosa_laevigata RLG00000007873 RLG00000007874
rosa_multiflora Rmu_sc0009909.1_g000002
rosa_roxburghii Rroxscaffold_5G00361530
rosa_rugosa Rorug04G0152300
rosa_samantha Rh4AG213900 Rh4BG216500
rosa_wichuraiana Rw4G018170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 269
AccII CGCG 1 cut(s) 23
AciI CCGC 3 cut(s) 21, 57, 69
AclWI GGATC 5 cut(s) 38, 138, 567, 717, 785
AcoI YGGCCR 1 cut(s) 159
AcsI RAATTY 1 cut(s) 590
AcuI CTGAAG 2 cut(s) 186, 819
AfaI GTAC 2 cut(s) 195, 207
AfiI CCNNNNNNNGG 4 cut(s) 27, 460, 582, 583
AflIII ACRYGT 1 cut(s) 441
AgsI TTSAA 4 cut(s) 92, 103, 343, 439
AhlI ACTAGT 1 cut(s) 532
AjnI CCWGG 1 cut(s) 754
AluBI AGCT 3 cut(s) 18, 200, 692
AluI AGCT 3 cut(s) 18, 200, 692
Alw21I GWGCWC 1 cut(s) 20
AlwI GGATC 5 cut(s) 38, 138, 567, 717, 785
AlwNI CAGNNNCTG 1 cut(s) 290
AoxI GGCC 4 cut(s) 136, 159, 235, 625
ApeKI GCWGC 3 cut(s) 290, 409, 677
ApoI RAATTY 1 cut(s) 590
Asp700I GAANNNNTTC 1 cut(s) 107
AspLEI GCGC 1 cut(s) 25
AspS9I GGNCC 3 cut(s) 137, 146, 625
AsuHPI GGTGA 2 cut(s) 475, 599
AsuII TTCGAA 1 cut(s) 596
AvaII GGWCC 1 cut(s) 146
BanI GGYRCC 1 cut(s) 269
BanII GRGCYC 1 cut(s) 20
BbsI GAAGAC 2 cut(s) 605, 807
Bbv12I GWGCWC 1 cut(s) 20
BbvCI CCTCAGC 1 cut(s) 26
BbvI GCAGC 3 cut(s) 277, 396, 664
BccI CCATC 1 cut(s) 813
BceAI ACGGC 1 cut(s) 146
BciT130I CCWGG 1 cut(s) 756
BciVI GTATCC 1 cut(s) 646
BcuI ACTAGT 1 cut(s) 532
BfaI CTAG 1 cut(s) 533
BfmI CTRYAG 1 cut(s) 276
BfuI GTATCC 1 cut(s) 646
BisI GCNGC 4 cut(s) 57, 291, 410, 678
BlsI GCNGC 4 cut(s) 58, 292, 411, 679
BmcAI AGTACT 1 cut(s) 207
Bme1390I CCNGG 1 cut(s) 756
Bme18I GGWCC 1 cut(s) 146
BmgT120I GGNCC 3 cut(s) 137, 146, 625
BmiI GGNNCC 2 cut(s) 147, 271
BmrFI CCNGG 1 cut(s) 756
BmrI ACTGGG 1 cut(s) 632
BmsI GCATC 1 cut(s) 504
BmuI ACTGGG 1 cut(s) 632
BpiI GAAGAC 2 cut(s) 605, 807
Bpu10I CCTNAGC 1 cut(s) 26
Bpu14I TTCGAA 1 cut(s) 596
BpuEI CTTGAG 1 cut(s) 539
BsaBI GATNNNNATC 2 cut(s) 129, 377
BsaJI CCNNGG 4 cut(s) 140, 214, 577, 672
BsaXI ACNNNNNCTCC 2 cut(s) 27, 57
Bsc4I CCNNNNNNNGG 4 cut(s) 27, 460, 582, 583
Bse1I ACTGG 1 cut(s) 627
Bse8I GATNNNNATC 2 cut(s) 129, 377
BseBI CCWGG 1 cut(s) 756
BseDI CCNNGG 4 cut(s) 140, 214, 577, 672
BseJI GATNNNNATC 2 cut(s) 129, 377
BseLI CCNNNNNNNGG 4 cut(s) 27, 460, 582, 583
BseMII CTCAG 3 cut(s) 17, 368, 723
BseNI ACTGG 1 cut(s) 627
BseRI GAGGAG 3 cut(s) 35, 38, 41
BseXI GCAGC 3 cut(s) 277, 396, 664
BsgI GTGCAG 2 cut(s) 303, 384
Bsh1236I CGCG 1 cut(s) 23
BshFI GGCC 4 cut(s) 138, 161, 237, 627
BshNI GGYRCC 1 cut(s) 269
BsiHKAI GWGCWC 1 cut(s) 20
BslI CCNNNNNNNGG 4 cut(s) 27, 460, 582, 583
BsmI GAATGC 1 cut(s) 293
BsnI GGCC 4 cut(s) 138, 161, 237, 627
Bsp119I TTCGAA 1 cut(s) 596
Bsp1286I GDGCHC 1 cut(s) 20
Bsp143I GATC 6 cut(s) 30, 130, 320, 559, 709, 790
Bsp19I CCATGG 1 cut(s) 577
BspACI CCGC 3 cut(s) 21, 57, 69
BspANI GGCC 4 cut(s) 138, 161, 237, 627
BspCNI CTCAG 3 cut(s) 18, 367, 724
BspFNI CGCG 1 cut(s) 23
BspHI TCATGA 1 cut(s) 808
BspLI GGNNCC 2 cut(s) 147, 271
BspPI GGATC 5 cut(s) 38, 138, 567, 717, 785
BspT104I TTCGAA 1 cut(s) 596
BspT107I GGYRCC 1 cut(s) 269
BsrI ACTGG 1 cut(s) 627
BssECI CCNNGG 4 cut(s) 140, 214, 577, 672
BssMI GATC 6 cut(s) 30, 130, 320, 559, 709, 790
BssT1I CCWWGG 2 cut(s) 577, 672
Bst2UI CCWGG 1 cut(s) 756
Bst4CI ACNGT 1 cut(s) 184
BstAPI GCANNNNNTGC 1 cut(s) 290
BstBI TTCGAA 1 cut(s) 596
BstC8I GCNNGC 3 cut(s) 159, 239, 495
BstDEI CTNAG 3 cut(s) 26, 354, 732
BstDSI CCRYGG 2 cut(s) 140, 577
BstFNI CGCG 1 cut(s) 23
BstHHI GCGC 1 cut(s) 25
BstKTI GATC 6 cut(s) 33, 133, 323, 562, 712, 793
BstMBI GATC 6 cut(s) 30, 130, 320, 559, 709, 790
BstMWI GCNNNNNNNGC 4 cut(s) 62, 197, 290, 677
BstNI CCWGG 1 cut(s) 756
BstNSI RCATGY 2 cut(s) 445, 493
BstSCI CCNGG 1 cut(s) 754
BstSFI CTRYAG 1 cut(s) 276
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 3 cut(s) 277, 396, 664
BstV2I GAAGAC 2 cut(s) 605, 807
BstX2I RGATCY 4 cut(s) 130, 559, 709, 790
BstYI RGATCY 4 cut(s) 130, 559, 709, 790
BsuI GTATCC 1 cut(s) 646
BsuRI GGCC 4 cut(s) 138, 161, 237, 627
BtgI CCRYGG 2 cut(s) 140, 577
Cac8I GCNNGC 3 cut(s) 159, 239, 495
CaiI CAGNNNCTG 1 cut(s) 290
CciI TCATGA 1 cut(s) 808
CfoI GCGC 1 cut(s) 25
Cfr13I GGNCC 3 cut(s) 137, 146, 625
CsiI ACCWGGT 1 cut(s) 754
Csp6I GTAC 2 cut(s) 194, 206
CviAII CATG 6 cut(s) 12, 442, 490, 501, 578, 809
CviQI GTAC 2 cut(s) 194, 206
DdeI CTNAG 3 cut(s) 26, 354, 732
DpnI GATC 6 cut(s) 32, 132, 322, 561, 711, 792
DpnII GATC 6 cut(s) 30, 130, 320, 559, 709, 790
EaeI YGGCCR 1 cut(s) 159
Ecl136II GAGCTC 1 cut(s) 18
Eco130I CCWWGG 2 cut(s) 577, 672
Eco24I GRGCYC 1 cut(s) 20
Eco32I GATATC 1 cut(s) 718
Eco47I GGWCC 1 cut(s) 146
Eco53kI GAGCTC 1 cut(s) 18
Eco57I CTGAAG 2 cut(s) 186, 819
EcoICRI GAGCTC 1 cut(s) 18
EcoRII CCWGG 1 cut(s) 754
EcoRV GATATC 1 cut(s) 718
EcoT14I CCWWGG 2 cut(s) 577, 672
EcoT38I GRGCYC 1 cut(s) 20
ErhI CCWWGG 2 cut(s) 577, 672
FaeI CATG 6 cut(s) 15, 445, 493, 504, 581, 812
FalI AAGNNNNNCTT 2 cut(s) 95, 127
FatI CATG 6 cut(s) 11, 441, 489, 500, 577, 808
Fnu4HI GCNGC 4 cut(s) 57, 291, 410, 678
FriOI GRGCYC 1 cut(s) 20
Fsp4HI GCNGC 4 cut(s) 57, 291, 410, 678
FspBI CTAG 1 cut(s) 533
GlaI GCGC 1 cut(s) 24
GluI GCNGC 4 cut(s) 57, 291, 410, 678
HaeIII GGCC 4 cut(s) 138, 161, 237, 627
HhaI GCGC 1 cut(s) 25
Hin1II CATG 6 cut(s) 15, 445, 493, 504, 581, 812
Hin6I GCGC 1 cut(s) 23
HinP1I GCGC 1 cut(s) 23
HindIII AAGCTT 1 cut(s) 690
HinfI GANTC 3 cut(s) 260, 466, 643
HphI GGTGA 2 cut(s) 475, 599
Hpy188I TCNGA 4 cut(s) 383, 568, 748, 799
Hpy188III TCNNGA 5 cut(s) 390, 713, 764, 784, 809
HpyAV CCTTC 4 cut(s) 50, 97, 308, 337
HpyCH4III ACNGT 1 cut(s) 184
HpyCH4V TGCA 6 cut(s) 167, 284, 293, 365, 377, 530
HpyF10VI GCNNNNNNNGC 4 cut(s) 62, 197, 290, 677
HpyF3I CTNAG 3 cut(s) 26, 354, 732
Hsp92II CATG 6 cut(s) 15, 445, 493, 504, 581, 812
HspAI GCGC 1 cut(s) 23
Kzo9I GATC 6 cut(s) 30, 130, 320, 559, 709, 790
LmnI GCTCC 2 cut(s) 15, 23
Lsp1109I GCAGC 3 cut(s) 277, 396, 664
LweI GCATC 1 cut(s) 504
MabI ACCWGGT 1 cut(s) 754
MaeI CTAG 1 cut(s) 533
MaeIII GTNAC 4 cut(s) 463, 571, 658, 735
MalI GATC 6 cut(s) 32, 132, 322, 561, 711, 792
MboI GATC 6 cut(s) 30, 130, 320, 559, 709, 790
MboII GAAGA 2 cut(s) 610, 812
MfeI CAATTG 2 cut(s) 264, 477
MflI RGATCY 4 cut(s) 130, 559, 709, 790
MhlI GDGCHC 1 cut(s) 20
MluCI AATT 4 cut(s) 264, 448, 477, 590
MlyI GAGTC 2 cut(s) 254, 460
MmeI TCCRAC 1 cut(s) 523
MnlI CCTC 9 cut(s) 21, 56, 59, 62, 224, 363, 464, 700, 805
MroXI GAANNNNTTC 1 cut(s) 107
MseI TTAA 4 cut(s) 179, 396, 446, 779
MspA1I CMGCKG 1 cut(s) 59
MspR9I CCNGG 1 cut(s) 756
MunI CAATTG 2 cut(s) 264, 477
Mva1269I GAATGC 1 cut(s) 293
MvaI CCWGG 1 cut(s) 756
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 4 cut(s) 62, 197, 290, 677
NcoI CCATGG 1 cut(s) 577
NdeII GATC 6 cut(s) 30, 130, 320, 559, 709, 790
NlaIII CATG 6 cut(s) 15, 445, 493, 504, 581, 812
NlaIV GGNNCC 2 cut(s) 147, 271
NmuCI GTSAC 2 cut(s) 463, 571
NspI RCATGY 2 cut(s) 445, 493
NspV TTCGAA 1 cut(s) 596
PagI TCATGA 1 cut(s) 808
PciI ACATGT 1 cut(s) 441
PctI GAATGC 1 cut(s) 293
PdmI GAANNNNTTC 1 cut(s) 107
PfeI GAWTC 1 cut(s) 643
PkrI GCNGC 4 cut(s) 58, 292, 411, 679
PleI GAGTC 2 cut(s) 254, 460
PpsI GAGTC 2 cut(s) 254, 460
PscI ACATGT 1 cut(s) 441
Psp124BI GAGCTC 1 cut(s) 20
Psp6I CCWGG 1 cut(s) 754
PspGI CCWGG 1 cut(s) 754
PspN4I GGNNCC 2 cut(s) 147, 271
PspPI GGNCC 3 cut(s) 137, 146, 625
PstNI CAGNNNCTG 1 cut(s) 290
PsuI RGATCY 4 cut(s) 130, 559, 709, 790
RsaI GTAC 2 cut(s) 195, 207
RsaNI GTAC 2 cut(s) 194, 206
SacI GAGCTC 1 cut(s) 20
SaqAI TTAA 4 cut(s) 179, 396, 446, 779
SatI GCNGC 4 cut(s) 57, 291, 410, 678
Sau3AI GATC 6 cut(s) 30, 130, 320, 559, 709, 790
Sau96I GGNCC 3 cut(s) 137, 146, 625
ScaI AGTACT 1 cut(s) 207
SchI GAGTC 2 cut(s) 254, 460
ScrFI CCNGG 1 cut(s) 756
SduI GDGCHC 1 cut(s) 20
SetI ASST 8 cut(s) 20, 202, 283, 456, 694, 733, 742, 757
SexAI ACCWGGT 1 cut(s) 754
SfaNI GCATC 1 cut(s) 504
SfcI CTRYAG 1 cut(s) 276
SfuI TTCGAA 1 cut(s) 596
SinI GGWCC 1 cut(s) 146
SmlI CTYRAG 1 cut(s) 554
SmoI CTYRAG 1 cut(s) 554
SpeI ACTAGT 1 cut(s) 532
Sse9I AATT 4 cut(s) 264, 448, 477, 590
SsiI CCGC 3 cut(s) 21, 57, 69
SspMI CTAG 1 cut(s) 533
SstI GAGCTC 1 cut(s) 20
StyD4I CCNGG 1 cut(s) 754
StyI CCWWGG 2 cut(s) 577, 672
TaaI ACNGT 1 cut(s) 184
TaqI TCGA 2 cut(s) 596, 765
TasI AATT 4 cut(s) 264, 448, 477, 590
TatI WGTACW 1 cut(s) 205
TauI GCSGC 1 cut(s) 59
TfiI GAWTC 1 cut(s) 643
Tru1I TTAA 4 cut(s) 179, 396, 446, 779
Tru9I TTAA 4 cut(s) 179, 396, 446, 779
TseFI GTSAC 2 cut(s) 463, 571
TseI GCWGC 3 cut(s) 290, 409, 677
Tsp45I GTSAC 2 cut(s) 463, 571
TspDTI ATGAA 1 cut(s) 489
TspGWI ACGGA 1 cut(s) 628
VpaK11BI GGWCC 1 cut(s) 146
XapI RAATTY 1 cut(s) 590
XceI RCATGY 2 cut(s) 445, 493
XcmI CCANNNNNNNNNTGG 1 cut(s) 679
XmnI GAANNNNTTC 1 cut(s) 107
XspI CTAG 1 cut(s) 533
ZrmI AGTACT 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.