pycom16g15450

TVP38 TMEM64 family membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr16
OG0010842
Physical Location & Seq
Forward (+)
11398584 .. 11401539
2956 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom16g15450.3

Sequence Viewer

Length: 555 bp
ATGGCGTTCACTTGGGCTTCTGCTCTCAGGACCACTCTCCTCCTCCTCCTTCTGGCCGCCGTTGCCACTGCTTGTTTTACTCTCCCTATTGAAAAGATTTTGAAGGACTTCTTATTATGGGTTGAGCATGAGCTTGGTCCTTGGGGTCCCCTTGTGCTGGCTATTGCATACATCCCACTAACAGTTTTGGCAGTACCAGCTTCAGTACTTACTCTCGGTGGTGGTTATCTTTTTGGCCTACCTGTTGGCTTTGTTGCCGACTCAATTGGAGCCACTATAGGTGCAGGGGCTGCTTTCCTTCTTGGAAGAACTTGGGAGATCATTTGTTGTTTCCAGATTGAAGGATTATCCTCAGTTCCGGGCAGTTGCCATTGCAATTCGGAGATCTGGATTCAAGATTGTTTTGTTGCTTCGTCTTGTGCCCTTGCTCCCCTTCAACATGCTAAATTACCTCCTCTCTGTGACTCCTGTTCCAATAGGACAATACATGCTGGCTTCCTGGATAGGGATGATGCCAATAACACTTGCGCTAGTATATGTTGGAACAACTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

185

Amino Acids

19.54

Weight (kDa)

4.93

Isoelectric Point (pI)

43.2

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010842)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19070 AT5G19070
fragaria_vesca FvH4_4g14490 FvH4_4g14490 FvH4_4g14490 FvH4_4g14490
malus_domestica MD13G1183100.v1.1 MD16G1184000.v1.1
prunus_persica Prupe.1G151400_v2.0.a1
pyrus_communis pycom13g15830 pycom16g15450
rosa_chinensis RchiOBHm_Chr4g0418341
rosa_laevigata RLG00000007873 RLG00000007874
rosa_multiflora Rmu_sc0009909.1_g000002
rosa_roxburghii Rroxscaffold_5G00361530
rosa_rugosa Rorug04G0152300
rosa_samantha Rh4AG213900 Rh4BG216500
rosa_wichuraiana Rw4G018170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 57
AcoI YGGCCR 1 cut(s) 54
AcuI CTGAAG 1 cut(s) 186
AfaI GTAC 2 cut(s) 195, 207
AfiI CCNNNNNNNGG 3 cut(s) 52, 157, 505
AgsI TTSAA 5 cut(s) 92, 103, 341, 395, 437
AjnI CCWGG 1 cut(s) 498
AluBI AGCT 2 cut(s) 133, 200
AluI AGCT 2 cut(s) 133, 200
AlwNI CAGNNNCTG 1 cut(s) 290
AoxI GGCC 2 cut(s) 54, 235
ApeKI GCWGC 1 cut(s) 290
Asp700I GAANNNNTTC 1 cut(s) 107
AspLEI GCGC 1 cut(s) 530
AspS9I GGNCC 3 cut(s) 30, 137, 146
AsuC2I CCSGG 1 cut(s) 360
AvaII GGWCC 3 cut(s) 30, 137, 146
BaeGI GKGCMC 1 cut(s) 424
BbvI GCAGC 1 cut(s) 277
BceAI ACGGC 1 cut(s) 44
BciT130I CCWGG 1 cut(s) 500
BcnI CCSGG 1 cut(s) 360
BfaI CTAG 1 cut(s) 531
BfmI CTRYAG 1 cut(s) 276
BglII AGATCT 1 cut(s) 384
BisI GCNGC 2 cut(s) 57, 291
BlsI GCNGC 2 cut(s) 58, 292
BmcAI AGTACT 1 cut(s) 207
Bme1390I CCNGG 2 cut(s) 360, 500
Bme18I GGWCC 3 cut(s) 30, 137, 146
BmgT120I GGNCC 3 cut(s) 30, 137, 146
BmiI GGNNCC 3 cut(s) 147, 148, 271
BmrFI CCNGG 2 cut(s) 360, 500
BmsI GCATC 1 cut(s) 502
BpuMI CCSGG 1 cut(s) 360
BsaJI CCNNGG 1 cut(s) 140
Bsc4I CCNNNNNNNGG 3 cut(s) 52, 157, 505
Bse3DI GCAATG 1 cut(s) 370
BseBI CCWGG 1 cut(s) 500
BseDI CCNNGG 1 cut(s) 140
BseGI GGATG 2 cut(s) 171, 514
BseLI CCNNNNNNNGG 3 cut(s) 52, 157, 505
BseMI GCAATG 1 cut(s) 370
BseMII CTCAG 2 cut(s) 40, 366
BseRI GAGGAG 4 cut(s) 29, 32, 35, 444
BseSI GKGCMC 1 cut(s) 424
BseXI GCAGC 1 cut(s) 277
BsgI GTGCAG 1 cut(s) 303
BshFI GGCC 2 cut(s) 56, 237
BsiSI CCGG 1 cut(s) 359
BslFI GGGAC 1 cut(s) 132
BslI CCNNNNNNNGG 3 cut(s) 52, 157, 505
BsmFI GGGAC 1 cut(s) 132
BsnI GGCC 2 cut(s) 56, 237
Bsp1286I GDGCHC 1 cut(s) 424
Bsp143I GATC 2 cut(s) 318, 384
BspACI CCGC 1 cut(s) 57
BspANI GGCC 2 cut(s) 56, 237
BspCNI CTCAG 2 cut(s) 39, 365
BspLI GGNNCC 3 cut(s) 147, 148, 271
BsrDI GCAATG 1 cut(s) 370
BssECI CCNNGG 1 cut(s) 140
BssMI GATC 2 cut(s) 318, 384
BssT1I CCWWGG 1 cut(s) 140
Bst2UI CCWGG 1 cut(s) 500
Bst4CI ACNGT 1 cut(s) 184
BstAPI GCANNNNNTGC 1 cut(s) 290
BstC8I GCNNGC 2 cut(s) 159, 493
BstDEI CTNAG 2 cut(s) 26, 352
BstF5I GGATG 2 cut(s) 171, 514
BstHHI GCGC 1 cut(s) 530
BstKTI GATC 2 cut(s) 321, 387
BstMBI GATC 2 cut(s) 318, 384
BstMWI GCNNNNNNNGC 3 cut(s) 62, 197, 290
BstNI CCWGG 1 cut(s) 500
BstNSI RCATGY 2 cut(s) 443, 491
BstSCI CCNGG 2 cut(s) 358, 498
BstSFI CTRYAG 1 cut(s) 276
BstSLI GKGCMC 1 cut(s) 424
BstV1I GCAGC 1 cut(s) 277
BstX2I RGATCY 1 cut(s) 384
BstYI RGATCY 1 cut(s) 384
BsuRI GGCC 2 cut(s) 56, 237
BtsCI GGATG 2 cut(s) 171, 514
BtsI GCAGTG 1 cut(s) 66
BtsIMutI CAGTG 1 cut(s) 66
Cac8I GCNNGC 2 cut(s) 159, 493
CaiI CAGNNNCTG 1 cut(s) 290
CfoI GCGC 1 cut(s) 530
Cfr13I GGNCC 3 cut(s) 30, 137, 146
Csp6I GTAC 2 cut(s) 194, 206
CviAII CATG 3 cut(s) 128, 440, 488
CviQI GTAC 2 cut(s) 194, 206
DdeI CTNAG 2 cut(s) 26, 352
DpnI GATC 2 cut(s) 320, 386
DpnII GATC 2 cut(s) 318, 384
EaeI YGGCCR 1 cut(s) 54
Eco130I CCWWGG 1 cut(s) 140
Eco47I GGWCC 3 cut(s) 30, 137, 146
Eco57I CTGAAG 1 cut(s) 186
EcoO109I RGGNCCY 1 cut(s) 146
EcoRII CCWGG 1 cut(s) 498
EcoT14I CCWWGG 1 cut(s) 140
ErhI CCWWGG 1 cut(s) 140
FaeI CATG 3 cut(s) 131, 443, 491
FaiI YATR 8 cut(s) 118, 129, 169, 278, 441, 489, 536, 538
FalI AAGNNNNNCTT 2 cut(s) 95, 127
FaqI GGGAC 1 cut(s) 132
FatI CATG 3 cut(s) 127, 439, 487
Fnu4HI GCNGC 2 cut(s) 57, 291
FokI GGATG 2 cut(s) 158, 521
Fsp4HI GCNGC 2 cut(s) 57, 291
FspBI CTAG 1 cut(s) 531
GlaI GCGC 1 cut(s) 529
GluI GCNGC 2 cut(s) 57, 291
HaeIII GGCC 2 cut(s) 56, 237
HapII CCGG 1 cut(s) 359
HhaI GCGC 1 cut(s) 530
Hin1II CATG 3 cut(s) 131, 443, 491
Hin6I GCGC 1 cut(s) 528
HinP1I GCGC 1 cut(s) 528
HinfI GANTC 3 cut(s) 260, 391, 464
HpaII CCGG 1 cut(s) 359
Hpy166II GTNNAC 1 cut(s) 9
Hpy188I TCNGA 1 cut(s) 382
Hpy188III TCNNGA 4 cut(s) 28, 334, 388, 395
Hpy8I GTNNAC 1 cut(s) 9
HpyAV CCTTC 5 cut(s) 59, 97, 308, 335, 443
HpyCH4III ACNGT 1 cut(s) 184
HpyCH4V TGCA 3 cut(s) 167, 284, 375
HpyF10VI GCNNNNNNNGC 3 cut(s) 62, 197, 290
HpyF3I CTNAG 2 cut(s) 26, 352
Hsp92II CATG 3 cut(s) 131, 443, 491
HspAI GCGC 1 cut(s) 528
KflI GGGWCCC 1 cut(s) 146
Kzo9I GATC 2 cut(s) 318, 384
LmnI GCTCC 2 cut(s) 269, 433
Lsp1109I GCAGC 1 cut(s) 277
LweI GCATC 1 cut(s) 502
MaeI CTAG 1 cut(s) 531
MaeIII GTNAC 1 cut(s) 461
MalI GATC 2 cut(s) 320, 386
MboI GATC 2 cut(s) 318, 384
MboII GAAGA 1 cut(s) 318
MfeI CAATTG 1 cut(s) 264
MflI RGATCY 1 cut(s) 384
MhlI GDGCHC 1 cut(s) 424
MluCI AATT 3 cut(s) 264, 376, 446
MlyI GAGTC 2 cut(s) 254, 458
MmeI TCCRAC 1 cut(s) 521
MnlI CCTC 6 cut(s) 50, 53, 56, 361, 462, 465
MroXI GAANNNNTTC 1 cut(s) 107
MseI TTAA 1 cut(s) 553
MspI CCGG 1 cut(s) 359
MspR9I CCNGG 2 cut(s) 360, 500
MunI CAATTG 1 cut(s) 264
MvaI CCWGG 1 cut(s) 500
MwoI GCNNNNNNNGC 3 cut(s) 62, 197, 290
NciI CCSGG 1 cut(s) 360
NdeII GATC 2 cut(s) 318, 384
NlaIII CATG 3 cut(s) 131, 443, 491
NlaIV GGNNCC 3 cut(s) 147, 148, 271
NmuCI GTSAC 1 cut(s) 461
NspI RCATGY 2 cut(s) 443, 491
PdmI GAANNNNTTC 1 cut(s) 107
PfeI GAWTC 1 cut(s) 391
PfoI TCCNGGA 1 cut(s) 498
PkrI GCNGC 2 cut(s) 58, 292
PleI GAGTC 2 cut(s) 254, 458
PpsI GAGTC 2 cut(s) 254, 458
PpuMI RGGWCCY 1 cut(s) 146
Psp5II RGGWCCY 1 cut(s) 146
Psp6I CCWGG 1 cut(s) 498
PspGI CCWGG 1 cut(s) 498
PspN4I GGNNCC 3 cut(s) 147, 148, 271
PspPI GGNCC 3 cut(s) 30, 137, 146
PspPPI RGGWCCY 1 cut(s) 146
PstNI CAGNNNCTG 1 cut(s) 290
PsuI RGATCY 1 cut(s) 384
RsaI GTAC 2 cut(s) 195, 207
RsaNI GTAC 2 cut(s) 194, 206
SaqAI TTAA 1 cut(s) 553
SatI GCNGC 2 cut(s) 57, 291
Sau3AI GATC 2 cut(s) 318, 384
Sau96I GGNCC 3 cut(s) 30, 137, 146
ScaI AGTACT 1 cut(s) 207
SchI GAGTC 2 cut(s) 254, 458
ScrFI CCNGG 2 cut(s) 360, 500
SduI GDGCHC 1 cut(s) 424
SetI ASST 5 cut(s) 135, 202, 244, 283, 454
SfaNI GCATC 1 cut(s) 502
SfcI CTRYAG 1 cut(s) 276
SinI GGWCC 3 cut(s) 30, 137, 146
Sse9I AATT 3 cut(s) 264, 376, 446
SsiI CCGC 1 cut(s) 57
SspMI CTAG 1 cut(s) 531
StyD4I CCNGG 2 cut(s) 358, 498
StyI CCWWGG 1 cut(s) 140
TaaI ACNGT 1 cut(s) 184
TasI AATT 3 cut(s) 264, 376, 446
TatI WGTACW 1 cut(s) 205
TauI GCSGC 1 cut(s) 59
TfiI GAWTC 1 cut(s) 391
Tru1I TTAA 1 cut(s) 553
Tru9I TTAA 1 cut(s) 553
TscAI CASTG 1 cut(s) 73
TseFI GTSAC 1 cut(s) 461
TseI GCWGC 1 cut(s) 290
Tsp45I GTSAC 1 cut(s) 461
TspRI CASTG 1 cut(s) 73
VpaK11BI GGWCC 3 cut(s) 30, 137, 146
XceI RCATGY 2 cut(s) 443, 491
XmnI GAANNNNTTC 1 cut(s) 107
XspI CTAG 1 cut(s) 531
ZrmI AGTACT 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.