Rh4BG216500

TVP38 TMEM64 family membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0010842
Physical Location & Seq
Forward (+)
37706839 .. 37711889
5051 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG216500.1

Sequence Viewer

Length: 663 bp
ATGGCTCTCACATGGGGCTCCGCGCTGAGGATCACACTCCTTCTCCTCCTCCTCGCCGCTGTTGCCACCGCCTGTTTCACTCTCCCCGTTGAAAAGATTTTGAAGGACTTCTTATTGTGGGTTGATAAGGATCTTGGCCCGTGGGGTCCATTTGTGCTGGCCATTGCATACATTCCTTTAACGGTCTTGGCAGTACCAGCTTCAGTACTTACCCTCGGTGGTGGTTATCTTTTTGGCCTGCCTGTCGGCTTTGTTGCCGACTCAATAGGTGCCACTATAGGTGCAGGGGCTGCATTCCTTCTTGGCAGAACTATTGGGAGATCATTCGTCATTTCCAAGTTGAAGGATTATCCTCAGTTTCGTGCAGTTGCGATTGCAATCCGAAAATCTGGATTTAAGATTGTTTTGCTACTTCGTCTTGTTCCCTTACTTCCATTCAACATGTTAAATTACCTCCTGTCGGTGACTCCTGTTCCAATTGGAGAATACATGCTTGCTTCATGGTTAGGAATGATGCCGATAACACTTGCACTAGTATATGTTGGAACGACTCTCAAGGATCTTTCTGATGTGACCCATGGTTGGGGTGAATTTTCGAAGACCCGTTGGGTAAGAGCATCTCATCTTTCATCTTCTGTGTTACTTGTAATTTTTAAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SNARE_assoc

Protein Analysis

220

Amino Acids

23.8

Weight (kDa)

9.85

Isoelectric Point (pI)

27.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VTT_dom PF09335 65 - 183 1.2e-24 VTT domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010842)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19070 AT5G19070
fragaria_vesca FvH4_4g14490 FvH4_4g14490 FvH4_4g14490 FvH4_4g14490
malus_domestica MD13G1183100.v1.1 MD16G1184000.v1.1
prunus_persica Prupe.1G151400_v2.0.a1
pyrus_communis pycom13g15830 pycom16g15450
rosa_chinensis RchiOBHm_Chr4g0418341
rosa_laevigata RLG00000007873 RLG00000007874
rosa_multiflora Rmu_sc0009909.1_g000002
rosa_roxburghii Rroxscaffold_5G00361530
rosa_rugosa Rorug04G0152300
rosa_samantha Rh4AG213900 Rh4BG216500
rosa_wichuraiana Rw4G018170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 269
AccII CGCG 1 cut(s) 23
AciI CCGC 3 cut(s) 21, 57, 69
AclWI GGATC 3 cut(s) 38, 138, 567
AcoI YGGCCR 1 cut(s) 159
AcsI RAATTY 1 cut(s) 590
AcuI CTGAAG 1 cut(s) 186
AfaI GTAC 2 cut(s) 195, 207
AfiI CCNNNNNNNGG 4 cut(s) 27, 460, 582, 583
AflIII ACRYGT 1 cut(s) 441
AgsI TTSAA 4 cut(s) 92, 103, 343, 439
AhlI ACTAGT 1 cut(s) 532
AluBI AGCT 1 cut(s) 200
AluI AGCT 1 cut(s) 200
AlwI GGATC 3 cut(s) 38, 138, 567
AlwNI CAGNNNCTG 1 cut(s) 290
AoxI GGCC 3 cut(s) 136, 159, 235
ApeKI GCWGC 1 cut(s) 290
ApoI RAATTY 1 cut(s) 590
Asp700I GAANNNNTTC 1 cut(s) 107
AspLEI GCGC 1 cut(s) 25
AspS9I GGNCC 2 cut(s) 137, 146
AsuHPI GGTGA 2 cut(s) 475, 599
AsuII TTCGAA 1 cut(s) 596
AvaII GGWCC 1 cut(s) 146
BalI TGGCCA 1 cut(s) 161
BanI GGYRCC 1 cut(s) 269
BanII GRGCYC 1 cut(s) 20
BbsI GAAGAC 1 cut(s) 605
BbvCI CCTCAGC 1 cut(s) 26
BbvI GCAGC 1 cut(s) 277
BcuI ACTAGT 1 cut(s) 532
BfaI CTAG 1 cut(s) 533
BfmI CTRYAG 1 cut(s) 276
BisI GCNGC 2 cut(s) 57, 291
BlsI GCNGC 2 cut(s) 58, 292
BmcAI AGTACT 1 cut(s) 207
Bme18I GGWCC 1 cut(s) 146
BmgT120I GGNCC 2 cut(s) 137, 146
BmiI GGNNCC 3 cut(s) 19, 147, 271
BmsI GCATC 2 cut(s) 504, 626
BpiI GAAGAC 1 cut(s) 605
Bpu10I CCTNAGC 1 cut(s) 26
Bpu14I TTCGAA 1 cut(s) 596
BpuEI CTTGAG 1 cut(s) 539
BsaBI GATNNNNATC 2 cut(s) 129, 377
BsaJI CCNNGG 3 cut(s) 140, 214, 577
BsaXI ACNNNNNCTCC 2 cut(s) 27, 57
Bsc4I CCNNNNNNNGG 4 cut(s) 27, 460, 582, 583
Bse3DI GCAATG 1 cut(s) 162
Bse8I GATNNNNATC 2 cut(s) 129, 377
BseDI CCNNGG 3 cut(s) 140, 214, 577
BseJI GATNNNNATC 2 cut(s) 129, 377
BseLI CCNNNNNNNGG 4 cut(s) 27, 460, 582, 583
BseMI GCAATG 1 cut(s) 162
BseMII CTCAG 2 cut(s) 17, 368
BseRI GAGGAG 3 cut(s) 35, 38, 41
BseXI GCAGC 1 cut(s) 277
BsgI GTGCAG 2 cut(s) 303, 384
Bsh1236I CGCG 1 cut(s) 23
BshFI GGCC 3 cut(s) 138, 161, 237
BshNI GGYRCC 1 cut(s) 269
BslI CCNNNNNNNGG 4 cut(s) 27, 460, 582, 583
BsmI GAATGC 1 cut(s) 293
BsnI GGCC 3 cut(s) 138, 161, 237
Bsp119I TTCGAA 1 cut(s) 596
Bsp1286I GDGCHC 1 cut(s) 20
Bsp143I GATC 4 cut(s) 30, 130, 320, 559
Bsp19I CCATGG 1 cut(s) 577
BspACI CCGC 3 cut(s) 21, 57, 69
BspANI GGCC 3 cut(s) 138, 161, 237
BspCNI CTCAG 2 cut(s) 18, 367
BspFNI CGCG 1 cut(s) 23
BspLI GGNNCC 3 cut(s) 19, 147, 271
BspPI GGATC 3 cut(s) 38, 138, 567
BspT104I TTCGAA 1 cut(s) 596
BspT107I GGYRCC 1 cut(s) 269
BsrDI GCAATG 1 cut(s) 162
BssECI CCNNGG 3 cut(s) 140, 214, 577
BssMI GATC 4 cut(s) 30, 130, 320, 559
BssT1I CCWWGG 1 cut(s) 577
Bst4CI ACNGT 1 cut(s) 184
BstAPI GCANNNNNTGC 1 cut(s) 290
BstBI TTCGAA 1 cut(s) 596
BstC8I GCNNGC 3 cut(s) 159, 239, 495
BstDEI CTNAG 2 cut(s) 26, 354
BstDSI CCRYGG 2 cut(s) 140, 577
BstFNI CGCG 1 cut(s) 23
BstHHI GCGC 1 cut(s) 25
BstKTI GATC 4 cut(s) 33, 133, 323, 562
BstMBI GATC 4 cut(s) 30, 130, 320, 559
BstMWI GCNNNNNNNGC 3 cut(s) 62, 197, 290
BstNSI RCATGY 2 cut(s) 445, 493
BstSFI CTRYAG 1 cut(s) 276
BstUI CGCG 1 cut(s) 23
BstV1I GCAGC 1 cut(s) 277
BstV2I GAAGAC 1 cut(s) 605
BstX2I RGATCY 2 cut(s) 130, 559
BstYI RGATCY 2 cut(s) 130, 559
BsuRI GGCC 3 cut(s) 138, 161, 237
BtgI CCRYGG 2 cut(s) 140, 577
Cac8I GCNNGC 3 cut(s) 159, 239, 495
CaiI CAGNNNCTG 1 cut(s) 290
CfoI GCGC 1 cut(s) 25
Cfr13I GGNCC 2 cut(s) 137, 146
Csp6I GTAC 2 cut(s) 194, 206
CviAII CATG 5 cut(s) 12, 442, 490, 501, 578
CviJI RGCY 8 cut(s) 5, 18, 138, 161, 200, 237, 249, 290
CviKI_1 RGCY 8 cut(s) 5, 18, 138, 161, 200, 237, 249, 290
CviQI GTAC 2 cut(s) 194, 206
DdeI CTNAG 2 cut(s) 26, 354
DpnI GATC 4 cut(s) 32, 132, 322, 561
DpnII GATC 4 cut(s) 30, 130, 320, 559
DraI TTTAAA 1 cut(s) 655
EaeI YGGCCR 1 cut(s) 159
Eco130I CCWWGG 1 cut(s) 577
Eco24I GRGCYC 1 cut(s) 20
Eco47I GGWCC 1 cut(s) 146
Eco57I CTGAAG 1 cut(s) 186
EcoT14I CCWWGG 1 cut(s) 577
EcoT38I GRGCYC 1 cut(s) 20
ErhI CCWWGG 1 cut(s) 577
FaeI CATG 5 cut(s) 15, 445, 493, 504, 581
FaiI YATR 9 cut(s) 13, 169, 278, 443, 491, 502, 538, 540, 579
FalI AAGNNNNNCTT 2 cut(s) 95, 127
FatI CATG 5 cut(s) 11, 441, 489, 500, 577
Fnu4HI GCNGC 2 cut(s) 57, 291
FriOI GRGCYC 1 cut(s) 20
Fsp4HI GCNGC 2 cut(s) 57, 291
FspBI CTAG 1 cut(s) 533
GlaI GCGC 1 cut(s) 24
GluI GCNGC 2 cut(s) 57, 291
HaeIII GGCC 3 cut(s) 138, 161, 237
HhaI GCGC 1 cut(s) 25
Hin1II CATG 5 cut(s) 15, 445, 493, 504, 581
Hin6I GCGC 1 cut(s) 23
HinP1I GCGC 1 cut(s) 23
HinfI GANTC 3 cut(s) 260, 466, 550
HphI GGTGA 2 cut(s) 475, 599
Hpy188I TCNGA 2 cut(s) 383, 568
Hpy188III TCNNGA 1 cut(s) 390
HpyAV CCTTC 4 cut(s) 50, 97, 308, 337
HpyCH4III ACNGT 1 cut(s) 184
HpyCH4V TGCA 6 cut(s) 167, 284, 293, 365, 377, 530
HpyF10VI GCNNNNNNNGC 3 cut(s) 62, 197, 290
HpyF3I CTNAG 2 cut(s) 26, 354
Hsp92II CATG 5 cut(s) 15, 445, 493, 504, 581
HspAI GCGC 1 cut(s) 23
Kzo9I GATC 4 cut(s) 30, 130, 320, 559
LmnI GCTCC 1 cut(s) 23
LpnPI CCDG 9 cut(s) 85, 143, 210, 251, 255, 270, 375, 470, 483
Lsp1109I GCAGC 1 cut(s) 277
LweI GCATC 2 cut(s) 504, 626
MaeI CTAG 1 cut(s) 533
MaeIII GTNAC 3 cut(s) 463, 571, 639
MalI GATC 4 cut(s) 32, 132, 322, 561
MboI GATC 4 cut(s) 30, 130, 320, 559
MboII GAAGA 2 cut(s) 610, 624
MfeI CAATTG 1 cut(s) 477
MflI RGATCY 2 cut(s) 130, 559
MhlI GDGCHC 1 cut(s) 20
MlsI TGGCCA 1 cut(s) 161
MluCI AATT 4 cut(s) 448, 477, 590, 648
MluNI TGGCCA 1 cut(s) 161
MlyI GAGTC 3 cut(s) 254, 460, 544
MmeI TCCRAC 1 cut(s) 523
MnlI CCTC 7 cut(s) 21, 56, 59, 62, 224, 363, 464
Mox20I TGGCCA 1 cut(s) 161
MroXI GAANNNNTTC 1 cut(s) 107
MscI TGGCCA 1 cut(s) 161
MseI TTAA 4 cut(s) 179, 396, 446, 654
Msp20I TGGCCA 1 cut(s) 161
MspA1I CMGCKG 1 cut(s) 59
MunI CAATTG 1 cut(s) 477
Mva1269I GAATGC 1 cut(s) 293
MvnI CGCG 1 cut(s) 23
MwoI GCNNNNNNNGC 3 cut(s) 62, 197, 290
NcoI CCATGG 1 cut(s) 577
NdeII GATC 4 cut(s) 30, 130, 320, 559
NlaIII CATG 5 cut(s) 15, 445, 493, 504, 581
NlaIV GGNNCC 3 cut(s) 19, 147, 271
NmuCI GTSAC 2 cut(s) 463, 571
NspI RCATGY 2 cut(s) 445, 493
NspV TTCGAA 1 cut(s) 596
PciI ACATGT 1 cut(s) 441
PctI GAATGC 1 cut(s) 293
PdmI GAANNNNTTC 1 cut(s) 107
PkrI GCNGC 2 cut(s) 58, 292
PleI GAGTC 3 cut(s) 254, 460, 544
PpsI GAGTC 3 cut(s) 254, 460, 544
PscI ACATGT 1 cut(s) 441
PspN4I GGNNCC 3 cut(s) 19, 147, 271
PspPI GGNCC 2 cut(s) 137, 146
PstNI CAGNNNCTG 1 cut(s) 290
PsuI RGATCY 2 cut(s) 130, 559
RsaI GTAC 2 cut(s) 195, 207
RsaNI GTAC 2 cut(s) 194, 206
SaqAI TTAA 4 cut(s) 179, 396, 446, 654
SatI GCNGC 2 cut(s) 57, 291
Sau3AI GATC 4 cut(s) 30, 130, 320, 559
Sau96I GGNCC 2 cut(s) 137, 146
ScaI AGTACT 1 cut(s) 207
SchI GAGTC 3 cut(s) 254, 460, 544
SduI GDGCHC 1 cut(s) 20
SetI ASST 4 cut(s) 202, 271, 283, 456
SfaNI GCATC 2 cut(s) 504, 626
SfcI CTRYAG 1 cut(s) 276
SfuI TTCGAA 1 cut(s) 596
SinI GGWCC 1 cut(s) 146
SmlI CTYRAG 1 cut(s) 554
SmoI CTYRAG 1 cut(s) 554
SpeI ACTAGT 1 cut(s) 532
Sse9I AATT 4 cut(s) 448, 477, 590, 648
SsiI CCGC 3 cut(s) 21, 57, 69
SspMI CTAG 1 cut(s) 533
StyI CCWWGG 1 cut(s) 577
TaaI ACNGT 1 cut(s) 184
TaqI TCGA 1 cut(s) 596
TasI AATT 4 cut(s) 448, 477, 590, 648
TatI WGTACW 1 cut(s) 205
TauI GCSGC 1 cut(s) 59
Tru1I TTAA 4 cut(s) 179, 396, 446, 654
Tru9I TTAA 4 cut(s) 179, 396, 446, 654
TseFI GTSAC 2 cut(s) 463, 571
TseI GCWGC 1 cut(s) 290
Tsp45I GTSAC 2 cut(s) 463, 571
TspDTI ATGAA 2 cut(s) 489, 618
VpaK11BI GGWCC 1 cut(s) 146
XapI RAATTY 1 cut(s) 590
XceI RCATGY 2 cut(s) 445, 493
XmnI GAANNNNTTC 1 cut(s) 107
XspI CTAG 1 cut(s) 533
ZrmI AGTACT 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.