Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0013045

Physical Location & Seq

Genomic Coordinates Forward (+)

16802602 .. 16804882

Gene Structure 2281 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1194200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2055 bp

ATGCCATTCCTCGCTCCCAACACGCTCGCCTCCCTGGTCGAATCCGCCGTGTCGACGCACTCCCCTCTGCTCGCCCGGGCGGCGCATGCCCACATGATCAGAACCCTCGGAGCGTCTCTCCCCTCCTTCCTCTCCAATCACCTCGTCAACATGTACTCCAAACTCGACCTCCCCGACTCAGCCCAACTCGTCCTCCGACTCAGCCCATCTCGCTCAGTCGTCACCTGGACCTCCCTCATCGCCGGTTCTGTCCAAAACGGCCATTTCGCCTCCGCTATCCTCCATTTCTCTAACATGCGCCGCGAGTCTATCCAGCCCAACGATTTCACCTTCCCTTGCGCCTTCAAGGCCTCGGGTTTGCTGCGGTTGCCTGTCACAGGAAAACAGCTCCATGCGCTAGCTGTCAAGGCCGGGCAGATATGCGATGTCTTTGTTGGGTGCAGCGCTTTTGATATGTACTGCAAGACGGGTCTCCGAGACGAGGCTCGGAATGTGTTTGATGAAATGCCCGAGAGAAACCTTGCGACGTGGAATGCGTACATGTCGAATGCGGTTCTTGACGGGCGGCCGCTAAATGCGGTTTACAAGTTTATTGAGTTTCTGCGGGCGGGTGGGGAGCCTAATTCGATTACGTTTTGTGCGTTTCTCAATGCATGTTCGGATACATCGAGTCTGGAGCTCGGGAAACAGTTACATGGGTTTGTGATGCGATGTGGGTTTGGCAAAGATGTGTCGGTTTTAAATGGGCTTATTGACTTTTATGGGAAGTGCCGGGAGGTCGGGTTTTCAATGACGGTTTTTTATGGAATTGGTCAGCGGAATGATGTTTCTTGGTGCTCTATGGTGGCTGCGTGCGTGCAGAATGATGAGGAGGAGAAGGCATGCGAGTTCTTTTTACGGGCGATGAAAGAAGGGGTTAAGCCGACTGACTTCATGGTTTCGAGTGTTCTAAGTGCTTGTGCTGGGGTTGCGTGGCTTGAACAGGGTAGGTCAGTTCACTCGGTTGCAGTGAAGGCGTGTGTGGAGGGAAATGTATTTGTTGGGAGTGCACTTGTTGACATGTATGGAAAATGTGGCTGCATAGAAGATGCCAAGCGTGCCTTTGATGAGATGCCTTCGAGGAACTTGATCACTTGGAACGCAATGGTAGGTGCGTACGCACATCAAGGGCTTGCCGATATGGCTTTGGCATTGTTCAAGGAAATGTGCACCCAAAGTCACGAGTTTAAACCAAATTATGTTACATTGGTGTGTGTATTATCGGCATGTAGTAGGGCAGGGGCTGTGCAAATGGGGATGCGGATCTTTGAGTCAATGAAAGCAAAGTATGGAATTGAACCAGGGGCAGAGCATTATGCATGTGTTGTGGACTTGCTAGGGCGAGCTGGGATGATAGAGCGAGCTTACGAGTTTATTACAAAGATGCCAATTCGGCCAACGATTTCCATTTGGGGGGCACTTCTTGGGGCTTGTAAAATGTATAGGAATACAGAATTGGGGAAGATTGCAGCTGACAAGTTATTTGAACTCGATCCTAAAGACTCCGGCAACCACGTGGTGCTTTCTAACATGTTTGCAGCTTGCGGCAGGTGGGAAGAAGCAACGCTTGTGAGAAAGGGAATGAAAGATGTTGGGATAAAAAAGGGTGCAGGCTACAGTTGGATCGCAGTCAAGAATGCAGTCCACGTCTTCCAAGCAAAGGACACCGCCCACGAAAGGAATTCAGAGATTCAGGCAATGCTAAGTGAGTTAAGGAGGAAAATGGACGAAGCTGGCTATATTGCTGACACCAATTTTGCGCTGTTTGATATAGAGGAAGAAGAGAAAGTGTCGGAAGTTTGGTACCACAGCGAGAAGATTGCGCTAGCGTTTGGACTCATATCCCTCCCTCCCGGGGTGCCTATAAGGATAACAAAGAACCTTAGGATCTGTGGGGATTGCCATGGTGCAATCAAGTTCATTTCTGGAATTGTAGGTAGAGAAATTATTGTGAGAGATAACAATCGGTTTCATCGCTTTAGAGATGGTCATTGCTCGTGTAGAGATTATTGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003727 GO:0003824 GO:0004518 GO:0004519 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0006081 GO:0006082 GO:0006084 GO:0006090 GO:0006139 GO:0006163 GO:0006629 GO:0006637 GO:0006644 GO:0006720 GO:0006725 GO:0006732 GO:0006753 GO:0006790 GO:0006793 GO:0006796 GO:0006807 GO:0007275 GO:0008150 GO:0008152 GO:0008202 GO:0008299 GO:0008610 GO:0008654 GO:0009058 GO:0009117 GO:0009150 GO:0009240 GO:0009259 GO:0009451 GO:0009987 GO:0016070 GO:0016125 GO:0016787 GO:0016788 GO:0019287 GO:0019288 GO:0019637 GO:0019682 GO:0019693 GO:0019752 GO:0022622 GO:0032501 GO:0032502 GO:0032787 GO:0033865 GO:0033875 GO:0034032 GO:0034046 GO:0034641 GO:0035383 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0043436 GO:0043603 GO:0044237 GO:0044238 GO:0044249 GO:0044255 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046483 GO:0046490 GO:0048364 GO:0048731 GO:0048856 GO:0050790 GO:0051186 GO:0055086 GO:0065007 GO:0065009 GO:0070717 GO:0071704 GO:0072521 GO:0090304 GO:0090305 GO:0090407 GO:0097159 GO:0099402 GO:1901135 GO:1901360 GO:1901363 GO:1901564 GO:1901576 GO:1901615

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DYW_deaminase PPR PPR_2

Protein Analysis

685

Amino Acids

75.48

Weight (kDa)

7.72

Isoelectric Point (pI)

41.39

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PPR_2	PF13041	72 - 116	4.2e-08	PPR repeat family
PPR	PF01535	377 - 403	2.3e-06	PPR repeat
E_motif	PF20431	494 - 555	2.9e-19	E motif
DYW_deaminase	PF14432	592 - 684	1e-39	DYW family of nucleic acid deaminases

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0013045)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G14850
fragaria_vesca	FvH4_6g27530
malus_domestica	MD13G1194200.v1.1
prunus_persica	Prupe.1G015100_v2.0.a1
pyrus_communis	pycom13g16650
rosa_chinensis	RchiOBHm_Chr2g0129921
rosa_laevigata	RLG00000019107 RLG00000019111
rosa_multiflora	Rmu_co8468659.1_g000001 Rmu_sc0009663.1_g000015
rosa_roxburghii	Rroxscaffold_2G00114230
rosa_rugosa	Rorug02G0290300 Rorug02G0290300 Rorug02G0290300
rosa_samantha	Rh2AG341700 Rh2BG349700 Rh2CG328900
rosa_wichuraiana	Rw2G027750

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1578
Acc36I	ACCTGC	1 cut(s)	1578
Acc65I	GGTACC	1 cut(s)	1842
AccB1I	GGYRCC	2 cut(s)	1842, 1897
AccI	GTMKAC	1 cut(s)	53
AccII	CGCG	1 cut(s)	303
AclWI	GGATC	4 cut(s)	1310, 1526, 1670, 1934
AcoI	YGGCCR	3 cut(s)	259, 566, 1433
AcsI	RAATTY	1 cut(s)	1720
AcvI	CACGTG	1 cut(s)	1555
AdeI	CACNNNGTG	1 cut(s)	1558
AfaI	GTAC	5 cut(s)	155, 458, 539, 1157, 1844
AfeI	AGCGCT	1 cut(s)	445
AfiI	CCNNNNNNNGG	6 cut(s)	377, 481, 1452, 1699, 1716, 1894
AflIII	ACRYGT	4 cut(s)	150, 540, 1059, 1569
AgsI	TTSAA	6 cut(s)	346, 789, 980, 1198, 1337, 1526
AjiI	CACGTC	2 cut(s)	528, 1687
AjnI	CCWGG	3 cut(s)	33, 224, 1339
AjuI	GAANNNNNNNTTGG	2 cut(s)	1478, 1510
AluBI	AGCT	8 cut(s)	388, 401, 679, 1385, 1403, 1511, 1580, 1772
AluI	AGCT	8 cut(s)	388, 401, 679, 1385, 1403, 1511, 1580, 1772
Alw21I	GWGCWC	4 cut(s)	681, 839, 1051, 1211
Alw26I	GTCTC	3 cut(s)	120, 471, 476
Alw44I	GTGCAC	2 cut(s)	1047, 1207
AlwI	GGATC	4 cut(s)	1310, 1526, 1670, 1934
AlwNI	CAGNNNCTG	1 cut(s)	1283
Ama87I	CYCGRG	5 cut(s)	75, 352, 509, 680, 1892
Aor51HI	AGCGCT	1 cut(s)	445
AoxI	GGCC	5 cut(s)	259, 348, 408, 566, 1433
ApaLI	GTGCAC	2 cut(s)	1047, 1207
ApeKI	GCWGC	6 cut(s)	361, 441, 848, 1077, 1508, 1577
ApoI	RAATTY	1 cut(s)	1720
ArsI	GACNNNNNNTTYG	4 cut(s)	1505, 1537, 1778, 1810
Asp718I	GGTACC	1 cut(s)	1842
AspLEI	GCGC	7 cut(s)	85, 300, 341, 397, 446, 1801, 1864
AspS9I	GGNCC	1 cut(s)	228
AsuC2I	CCSGG	6 cut(s)	76, 77, 412, 773, 1893, 1894
AsuHPI	GGTGA	3 cut(s)	131, 214, 319
AsuNHI	GCTAGC	2 cut(s)	397, 1864
AvaI	CYCGRG	5 cut(s)	75, 352, 509, 680, 1892
AvaII	GGWCC	1 cut(s)	228
AxyI	CCTNAGG	1 cut(s)	1922
BaeGI	GKGCMC	3 cut(s)	1051, 1211, 1459
BanI	GGYRCC	2 cut(s)	1842, 1897
BanII	GRGCYC	1 cut(s)	681
BauI	CACGAG	2 cut(s)	1220, 2035
BbrPI	CACGTG	1 cut(s)	1555
BbsI	GAAGAC	1 cut(s)	1681
Bbv12I	GWGCWC	4 cut(s)	681, 839, 1051, 1211
BbvI	GCAGC	6 cut(s)	348, 453, 835, 1064, 1520, 1589
BccI	CCATC	2 cut(s)	214, 2018
BceAI	ACGGC	2 cut(s)	32, 274
BcgI	CGANNNNNNTGC	2 cut(s)	1841, 1875
BciT130I	CCWGG	3 cut(s)	35, 226, 1341
BciVI	GTATCC	1 cut(s)	655
BclI	TGATCA	2 cut(s)	96, 1128
BcnI	CCSGG	6 cut(s)	76, 77, 412, 773, 1893, 1894
BcoDI	GTCTC	3 cut(s)	120, 471, 476
BfaI	CTAG	3 cut(s)	398, 1376, 1865
BfmI	CTRYAG	1 cut(s)	1654
BfoI	RGCGCY	1 cut(s)	447
BfuAI	ACCTGC	1 cut(s)	1578
BfuI	GTATCC	1 cut(s)	655
BglI	GCCNNNNNGGC	4 cut(s)	80, 347, 1181, 1432
Bme1390I	CCNGG	9 cut(s)	35, 76, 77, 226, 412, 773, 1341, 1893, 1894
Bme18I	GGWCC	1 cut(s)	228
BmeT110I	CYCGRG	5 cut(s)	75, 352, 509, 680, 1892
BmgBI	CACGTC	2 cut(s)	528, 1687
BmgT120I	GGNCC	1 cut(s)	228
BmiI	GGNNCC	3 cut(s)	618, 1844, 1899
BmrFI	CCNGG	9 cut(s)	35, 76, 77, 226, 412, 773, 1341, 1893, 1894
BmsI	GCATC	5 cut(s)	696, 1078, 1101, 1287, 1413
BmtI	GCTAGC	2 cut(s)	401, 1868
BpiI	GAAGAC	1 cut(s)	1681
BplI	GAGNNNNNCTC	2 cut(s)	102, 134
BpmI	CTGGAG	1 cut(s)	695
BpuMI	CCSGG	6 cut(s)	76, 77, 412, 773, 1893, 1894
BsaAI	YACGTR	1 cut(s)	1555
BsaBI	GATNNNNATC	2 cut(s)	1301, 2001
BsaI	GGTCTC	1 cut(s)	476
BsaJI	CCNNGG	8 cut(s)	33, 75, 106, 351, 1340, 1892, 1893, 1942
BsaXI	ACNNNNNCTCC	8 cut(s)	140, 153, 170, 177, 183, 207, 767, 797
Bsc4I	CCNNNNNNNGG	6 cut(s)	377, 481, 1452, 1699, 1716, 1894
Bse118I	RCCGGY	1 cut(s)	242
Bse21I	CCTNAGG	1 cut(s)	1922
Bse3DI	GCAATG	3 cut(s)	1149, 1743, 2029
Bse8I	GATNNNNATC	2 cut(s)	1301, 2001
BseBI	CCWGG	3 cut(s)	35, 226, 1341
BseDI	CCNNGG	8 cut(s)	33, 75, 106, 351, 1340, 1892, 1893, 1942
BseGI	GGATG	2 cut(s)	1302, 1395
BseJI	GATNNNNATC	2 cut(s)	1301, 2001
BseLI	CCNNNNNNNGG	6 cut(s)	377, 481, 1452, 1699, 1716, 1894
BseMI	GCAATG	3 cut(s)	1149, 1743, 2029
BseMII	CTCAG	3 cut(s)	192, 214, 228
BseRI	GAGGAG	2 cut(s)	884, 887
BseSI	GKGCMC	3 cut(s)	1051, 1211, 1459
BseX3I	CGGCCG	1 cut(s)	566
BseXI	GCAGC	6 cut(s)	348, 453, 835, 1064, 1520, 1589
BseYI	CCCAGC	2 cut(s)	962, 1385
BsgI	GTGCAG	3 cut(s)	460, 878, 1668
Bsh1236I	CGCG	1 cut(s)	303
Bsh1285I	CGRYCG	1 cut(s)	569
BshFI	GGCC	5 cut(s)	261, 350, 410, 568, 1435
BshNI	GGYRCC	2 cut(s)	1842, 1897
BsiEI	CGRYCG	1 cut(s)	569
BsiHKAI	GWGCWC	4 cut(s)	681, 839, 1051, 1211
BsiHKCI	CYCGRG	5 cut(s)	75, 352, 509, 680, 1892
BsiSI	CCGG	6 cut(s)	76, 243, 411, 772, 1545, 1893
BsiWI	CGTACG	1 cut(s)	1155
BslI	CCNNNNNNNGG	6 cut(s)	377, 481, 1452, 1699, 1716, 1894
BsmAI	GTCTC	3 cut(s)	120, 471, 476
BsmBI	CGTCTC	2 cut(s)	120, 471
BsmI	GAATGC	3 cut(s)	538, 553, 1681
BsnI	GGCC	5 cut(s)	261, 350, 410, 568, 1435
Bso31I	GGTCTC	1 cut(s)	476
BsoBI	CYCGRG	5 cut(s)	75, 352, 509, 680, 1892
Bsp1286I	GDGCHC	5 cut(s)	681, 839, 1051, 1211, 1459
Bsp143I	GATC	6 cut(s)	96, 1128, 1302, 1531, 1662, 1926
Bsp19I	CCATGG	1 cut(s)	1942
BspANI	GGCC	5 cut(s)	261, 350, 410, 568, 1435
BspCNI	CTCAG	3 cut(s)	191, 213, 227
BspFNI	CGCG	1 cut(s)	303
BspLI	GGNNCC	3 cut(s)	618, 1844, 1899
BspMI	ACCTGC	1 cut(s)	1578
BspOI	GCTAGC	2 cut(s)	401, 1868
BspPI	GGATC	4 cut(s)	1310, 1526, 1670, 1934
BspT107I	GGYRCC	2 cut(s)	1842, 1897
BspTNI	GGTCTC	1 cut(s)	476
BsrDI	GCAATG	3 cut(s)	1149, 1743, 2029
BsrFI	RCCGGY	1 cut(s)	242
BssAI	RCCGGY	1 cut(s)	242
BssECI	CCNNGG	8 cut(s)	33, 75, 106, 351, 1340, 1892, 1893, 1942
BssMI	GATC	6 cut(s)	96, 1128, 1302, 1531, 1662, 1926
BssSI	CACGAG	2 cut(s)	1220, 2035
BssT1I	CCWWGG	1 cut(s)	1942
Bst2BI	CACGAG	2 cut(s)	1220, 2035
Bst2UI	CCWGG	3 cut(s)	35, 226, 1341
Bst4CI	ACNGT	3 cut(s)	690, 796, 1658
Bst6I	CTCTTC	1 cut(s)	1815
BstBAI	YACGTR	1 cut(s)	1555
BstDEI	CTNAG	6 cut(s)	178, 200, 214, 950, 1742, 1922
BstDSI	CCRYGG	1 cut(s)	1942
BstENI	CCTNNNNNAGG	1 cut(s)	375
BstF5I	GGATG	2 cut(s)	1302, 1395
BstFNI	CGCG	1 cut(s)	303
BstH2I	RGCGCY	1 cut(s)	447
BstHHI	GCGC	7 cut(s)	85, 300, 341, 397, 446, 1801, 1864
BstKTI	GATC	6 cut(s)	99, 1131, 1305, 1534, 1665, 1929
BstMAI	GTCTC	3 cut(s)	120, 471, 476
BstMBI	GATC	6 cut(s)	96, 1128, 1302, 1531, 1662, 1926
BstMCI	CGRYCG	1 cut(s)	569
BstNI	CCWGG	3 cut(s)	35, 226, 1341
BstSCI	CCNGG	9 cut(s)	33, 74, 75, 224, 410, 771, 1339, 1891, 1892
BstSFI	CTRYAG	1 cut(s)	1654
BstSLI	GKGCMC	3 cut(s)	1051, 1211, 1459
BstUI	CGCG	1 cut(s)	303
BstV1I	GCAGC	6 cut(s)	348, 453, 835, 1064, 1520, 1589
BstV2I	GAAGAC	1 cut(s)	1681
BstX2I	RGATCY	2 cut(s)	1302, 1926
BstYI	RGATCY	2 cut(s)	1302, 1926
BstZI	CGGCCG	1 cut(s)	566
Bsu36I	CCTNAGG	1 cut(s)	1922
BsuI	GTATCC	1 cut(s)	655
BsuRI	GGCC	5 cut(s)	261, 350, 410, 568, 1435
BtgI	CCRYGG	1 cut(s)	1942
BtgZI	GCGATG	5 cut(s)	223, 438, 724, 917, 1997
BtrI	CACGTC	2 cut(s)	528, 1687
BtsCI	GGATG	2 cut(s)	1302, 1395
BtsI	GCAGTG	1 cut(s)	1014
BtsIMutI	CAGTG	1 cut(s)	1014
BveI	ACCTGC	1 cut(s)	1578
CaiI	CAGNNNCTG	1 cut(s)	1283
CciNI	GCGGCCGC	1 cut(s)	566
CfoI	GCGC	7 cut(s)	85, 300, 341, 397, 446, 1801, 1864
Cfr10I	RCCGGY	1 cut(s)	242
Cfr13I	GGNCC	1 cut(s)	228
Cfr9I	CCCGGG	2 cut(s)	75, 1892
CseI	GACGC	2 cut(s)	64, 102
Csp6I	GTAC	5 cut(s)	154, 457, 538, 1156, 1843
CviQI	GTAC	5 cut(s)	154, 457, 538, 1156, 1843
DdeI	CTNAG	6 cut(s)	178, 200, 214, 950, 1742, 1922
DpnI	GATC	6 cut(s)	98, 1130, 1304, 1533, 1664, 1928
DpnII	GATC	6 cut(s)	96, 1128, 1302, 1531, 1662, 1926
DraI	TTTAAA	2 cut(s)	741, 1228
DraIII	CACNNNGTG	1 cut(s)	1558
EaeI	YGGCCR	3 cut(s)	259, 566, 1433
EagI	CGGCCG	1 cut(s)	566
Eam1104I	CTCTTC	1 cut(s)	1815
EarI	CTCTTC	1 cut(s)	1815
EciI	GGCGGA	1 cut(s)	34
Ecl136II	GAGCTC	1 cut(s)	679
EclXI	CGGCCG	1 cut(s)	566
Eco130I	CCWWGG	1 cut(s)	1942
Eco147I	AGGCCT	1 cut(s)	350
Eco24I	GRGCYC	1 cut(s)	681
Eco31I	GGTCTC	1 cut(s)	476
Eco47I	GGWCC	1 cut(s)	228
Eco47III	AGCGCT	1 cut(s)	445
Eco52I	CGGCCG	1 cut(s)	566
Eco53kI	GAGCTC	1 cut(s)	679
Eco72I	CACGTG	1 cut(s)	1555
Eco81I	CCTNAGG	1 cut(s)	1922
Eco88I	CYCGRG	5 cut(s)	75, 352, 509, 680, 1892
EcoICRI	GAGCTC	1 cut(s)	679
EcoNI	CCTNNNNNAGG	1 cut(s)	375
EcoRI	GAATTC	1 cut(s)	1720
EcoRII	CCWGG	3 cut(s)	33, 224, 1339
EcoT14I	CCWWGG	1 cut(s)	1942
EcoT22I	ATGCAT	2 cut(s)	655, 1360
EcoT38I	GRGCYC	1 cut(s)	681
ErhI	CCWWGG	1 cut(s)	1942
Esp3I	CGTCTC	2 cut(s)	120, 471
FalI	AAGNNNNNCTT	4 cut(s)	1085, 1117, 1590, 1622
FauI	CCCGC	2 cut(s)	597, 601
FbaI	TGATCA	2 cut(s)	96, 1128
FblI	GTMKAC	1 cut(s)	53
FokI	GGATG	2 cut(s)	1309, 1402
FriOI	GRGCYC	1 cut(s)	681
FspBI	CTAG	3 cut(s)	398, 1376, 1865
GlaI	GCGC	7 cut(s)	84, 299, 340, 396, 445, 1800, 1863
GsaI	CCCAGC	2 cut(s)	966, 1389
GsuI	CTGGAG	1 cut(s)	695
HaeII	RGCGCY	1 cut(s)	447
HaeIII	GGCC	5 cut(s)	261, 350, 410, 568, 1435
HapII	CCGG	6 cut(s)	76, 243, 411, 772, 1545, 1893
HgaI	GACGC	2 cut(s)	64, 102
HhaI	GCGC	7 cut(s)	85, 300, 341, 397, 446, 1801, 1864
Hin6I	GCGC	7 cut(s)	83, 298, 339, 395, 444, 1799, 1862
HinP1I	GCGC	7 cut(s)	83, 298, 339, 395, 444, 1799, 1862
HincII	GTYRAC	3 cut(s)	54, 148, 1057
HindII	GTYRAC	3 cut(s)	54, 148, 1057
HinfI	GANTC	9 cut(s)	41, 176, 198, 305, 670, 1310, 1541, 1729, 1875
HpaII	CCGG	6 cut(s)	76, 243, 411, 772, 1545, 1893
HphI	GGTGA	3 cut(s)	131, 214, 319
Hpy166II	GTNNAC	9 cut(s)	54, 148, 583, 997, 1049, 1057, 1209, 1369, 1684
Hpy188I	TCNGA	8 cut(s)	101, 110, 197, 476, 489, 661, 1726, 1834
Hpy188III	TCNNGA	6 cut(s)	557, 674, 682, 1220, 1672, 1965
Hpy8I	GTNNAC	9 cut(s)	54, 148, 583, 997, 1049, 1057, 1209, 1369, 1684
Hpy99I	CGWCG	2 cut(s)	58, 529
HpyAV	CCTTC	7 cut(s)	136, 340, 352, 871, 905, 1006, 1125
HpyCH4III	ACNGT	3 cut(s)	690, 796, 1658
HpyCH4IV	ACGT	4 cut(s)	527, 632, 1554, 1686
HpyF3I	CTNAG	6 cut(s)	178, 200, 214, 950, 1742, 1922
HpySE526I	ACGT	4 cut(s)	527, 632, 1554, 1686
HspAI	GCGC	7 cut(s)	83, 298, 339, 395, 444, 1799, 1862
KpnI	GGTACC	1 cut(s)	1846
Ksp22I	TGATCA	2 cut(s)	96, 1128
Kzo9I	GATC	6 cut(s)	96, 1128, 1302, 1531, 1662, 1926
LmnI	GCTCC	5 cut(s)	19, 110, 393, 616, 676
Lsp1109I	GCAGC	6 cut(s)	348, 453, 835, 1064, 1520, 1589
LweI	GCATC	5 cut(s)	696, 1078, 1101, 1287, 1413
MaeI	CTAG	3 cut(s)	398, 1376, 1865
MaeII	ACGT	4 cut(s)	527, 632, 1554, 1686
MaeIII	GTNAC	5 cut(s)	220, 373, 690, 1217, 1240
MalI	GATC	6 cut(s)	98, 1130, 1304, 1533, 1664, 1928
MboI	GATC	6 cut(s)	96, 1128, 1302, 1531, 1662, 1926
MboII	GAAGA	7 cut(s)	1097, 1513, 1607, 1681, 1829, 1832, 1867
MflI	RGATCY	2 cut(s)	1302, 1926
MhlI	GDGCHC	5 cut(s)	681, 839, 1051, 1211, 1459
MlyI	GAGTC	7 cut(s)	170, 192, 314, 679, 1319, 1535, 1869
MmeI	TCCRAC	3 cut(s)	220, 1640, 1812
Mph1103I	ATGCAT	2 cut(s)	655, 1360
MseI	TTAA	4 cut(s)	740, 918, 1227, 1751
MslI	CAYNNNNRTG	1 cut(s)	1249
MspA1I	CMGCKG	2 cut(s)	817, 1511
MspI	CCGG	6 cut(s)	76, 243, 411, 772, 1545, 1893
MspR9I	CCNGG	9 cut(s)	35, 76, 77, 226, 412, 773, 1341, 1893, 1894
MssI	GTTTAAAC	1 cut(s)	1228
Mva1269I	GAATGC	3 cut(s)	538, 553, 1681
MvaI	CCWGG	3 cut(s)	35, 226, 1341
MvnI	CGCG	1 cut(s)	303
NciI	CCSGG	6 cut(s)	76, 77, 412, 773, 1893, 1894
NcoI	CCATGG	1 cut(s)	1942
NdeII	GATC	6 cut(s)	96, 1128, 1302, 1531, 1662, 1926
NheI	GCTAGC	2 cut(s)	397, 1864
NlaIV	GGNNCC	3 cut(s)	618, 1844, 1899
NmuCI	GTSAC	3 cut(s)	220, 373, 1217
NotI	GCGGCCGC	1 cut(s)	566
NsiI	ATGCAT	2 cut(s)	655, 1360
PaeI	GCATGC	2 cut(s)	89, 885
PaqCI	CACCTGC	1 cut(s)	1578
PceI	AGGCCT	1 cut(s)	350
PciI	ACATGT	4 cut(s)	150, 540, 1059, 1569
PcsI	WCGNNNNNNNCGW	5 cut(s)	45, 171, 533, 638, 665
PctI	GAATGC	3 cut(s)	538, 553, 1681
PfeI	GAWTC	2 cut(s)	41, 1729
Pfl23II	CGTACG	1 cut(s)	1155
PleI	GAGTC	7 cut(s)	170, 192, 313, 678, 1318, 1535, 1869
PmaCI	CACGTG	1 cut(s)	1555
PmeI	GTTTAAAC	1 cut(s)	1228
PmlI	CACGTG	1 cut(s)	1555
PpsI	GAGTC	7 cut(s)	170, 192, 313, 678, 1318, 1535, 1869
Ppu21I	YACGTR	1 cut(s)	1555
PscI	ACATGT	4 cut(s)	150, 540, 1059, 1569
Psp124BI	GAGCTC	1 cut(s)	681
Psp6I	CCWGG	3 cut(s)	33, 224, 1339
PspCI	CACGTG	1 cut(s)	1555
PspFI	CCCAGC	2 cut(s)	962, 1385
PspGI	CCWGG	3 cut(s)	33, 224, 1339
PspLI	CGTACG	1 cut(s)	1155
PspN4I	GGNNCC	3 cut(s)	618, 1844, 1899
PspPI	GGNCC	1 cut(s)	228
PstNI	CAGNNNCTG	1 cut(s)	1283
PsuI	RGATCY	2 cut(s)	1302, 1926
PvuII	CAGCTG	1 cut(s)	1511
RsaI	GTAC	5 cut(s)	155, 458, 539, 1157, 1844
RsaNI	GTAC	5 cut(s)	154, 457, 538, 1156, 1843
RseI	CAYNNNNRTG	1 cut(s)	1249
SacI	GAGCTC	1 cut(s)	681
SalI	GTCGAC	1 cut(s)	52
SaqAI	TTAA	4 cut(s)	740, 918, 1227, 1751
Sau3AI	GATC	6 cut(s)	96, 1128, 1302, 1531, 1662, 1926
Sau96I	GGNCC	1 cut(s)	228
SchI	GAGTC	7 cut(s)	170, 192, 314, 679, 1319, 1535, 1869
ScrFI	CCNGG	9 cut(s)	35, 76, 77, 226, 412, 773, 1341, 1893, 1894
SduI	GDGCHC	5 cut(s)	681, 839, 1051, 1211, 1459
SfaNI	GCATC	5 cut(s)	696, 1078, 1101, 1287, 1413
SfcI	CTRYAG	1 cut(s)	1654
SinI	GGWCC	1 cut(s)	228
SmaI	CCCGGG	2 cut(s)	77, 1894
SmiMI	CAYNNNNRTG	1 cut(s)	1249
SphI	GCATGC	2 cut(s)	89, 885
SrfI	GCCCGGGC	1 cut(s)	77
SseBI	AGGCCT	1 cut(s)	350
SspMI	CTAG	3 cut(s)	398, 1376, 1865
SstI	GAGCTC	1 cut(s)	681
StuI	AGGCCT	1 cut(s)	350
StyD4I	CCNGG	9 cut(s)	33, 74, 75, 224, 410, 771, 1339, 1891, 1892
StyI	CCWWGG	1 cut(s)	1942
TaaI	ACNGT	3 cut(s)	690, 796, 1658
TaiI	ACGT	4 cut(s)	530, 635, 1557, 1689
TaqI	TCGA	9 cut(s)	39, 53, 165, 545, 626, 668, 941, 1118, 1530
TatI	WGTACW	2 cut(s)	153, 456
TauI	GCSGC	5 cut(s)	83, 303, 568, 571, 1587
TfiI	GAWTC	2 cut(s)	41, 1729
Tru1I	TTAA	4 cut(s)	740, 918, 1227, 1751
Tru9I	TTAA	4 cut(s)	740, 918, 1227, 1751
TscAI	CASTG	1 cut(s)	1014
TseFI	GTSAC	3 cut(s)	220, 373, 1217
TseI	GCWGC	6 cut(s)	361, 441, 848, 1077, 1508, 1577
Tsp45I	GTSAC	3 cut(s)	220, 373, 1217
TspDTI	ATGAA	7 cut(s)	516, 920, 922, 1331, 1637, 1948, 2000
TspMI	CCCGGG	2 cut(s)	75, 1892
TspRI	CASTG	1 cut(s)	1014
VneI	GTGCAC	2 cut(s)	1047, 1207
VpaK11BI	GGWCC	1 cut(s)	228
XagI	CCTNNNNNAGG	1 cut(s)	375
XapI	RAATTY	1 cut(s)	1720
XmaI	CCCGGG	2 cut(s)	75, 1892
XmiI	GTMKAC	1 cut(s)	53
XspI	CTAG	3 cut(s)	398, 1376, 1865
Zsp2I	ATGCAT	2 cut(s)	655, 1360

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.