MD13G1202900.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0011029
Physical Location & Seq
Forward (+)
18252277 .. 18256084
3808 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1202900.v1.1.491

Sequence Viewer

Length: 1221 bp
ATGCTCCTCGCACCACCCGGCCATTGCCTTTTTCCGCTTCGCCGCATCCACAAACCCTACTCACTAAGCAGAACTCGGACGACTCGGTTCTGCTGCAGGGCAAGTCTGAATCTGATCACGAACTCTGACTCGTTCGAGGTGGGTCGACTTATTGGGAGCTATGGCTTCATGAATATCACTAGCTCTTCGGGGTCTCCTTTGGGCGCGGATGCTGAGTACTCATCGGGGGATGTGGGGCGGTTGAGAGTCCAAGATGTAGGAGAAGGCAGTGTAAAGATCAGGCTGTATGATGGGAGAGTCTCTCAGGGTCCGCTGAGAGGGACTCCAGTTGTTTTTAAGGTTTATCCTGGACAACGGGCTGGTGGGAATGAGGCAGATATGATGGCTGCTAATGAGCTAAATGCTCACTTATTCCTTCAAAACAATTCAAATGACATCTGTCAGAATCTTGTGTTACTCATAGGTGGGTTTGAAACGAAAACTGGGGAGCAGTGGCTTGCTTTTCGCAATCACGGGAAAGCTAGTGCAGCAGATTATGCCAAAGTGATGAGTGAAAGAGTGTCAAAAACCCGTGCTGACGGAGGACAAGTTTGGAATAATTTTGAACAAGAGGAAACTATCAAACGCAGAAGATATTTTGTTACAAAGCTGCTGCAGGGCATTATGAGAGGTTTAGCTTACATGCATGATCGTGAGAGGTTGCACCAGAGTCTTGGACCAGCTTCTGTTATTCTTAACACAATTGTAGAGAGAGATGCTGTTTACTTAGTCCCTCGACTTCGGGATCTAGCATTTTCTGTTGACATTGGGTACACAAAGCTAGAAGGGGATCCTGGATTACTCTCGGATGGACTTTGGAGACGAGCGTCTACTGCTGGTGCATTCACTCCTATGGAAAAAAGAGCCTTCGGAATAGCAGATGACATATATGAGGCAGGTCTTCTCTTGGCATACTTAGCATTCTTACCATTCTGTGAAGCAGGGATAATGGATGGCCTTTCGTTGCAAAGGCTTTTGGAGAGCACTTTCCAACTTGATATCGGAGCAACAAGAGAATACTGTTTAGCAGATGACCGGCTATTAGATGCCGTAAATTTTCTGGATCTTGGAGATGGTGCTGGTTGGGAGTTACTCCAGGCAATGTTGAATCCAGACTTCCGAAAACGGCCAACTGCACAGGCTGTTCTGAATCATCGGTTCATGACGGTTGGCAGCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

407

Amino Acids

44.97

Weight (kDa)

7.64

Isoelectric Point (pI)

34.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 926
AccI GTMKAC 2 cut(s) 145, 869
AccII CGCG 1 cut(s) 206
AciI CCGC 5 cut(s) 35, 43, 206, 238, 311
AclWI GGATC 4 cut(s) 792, 824, 837, 1110
AcoI YGGCCR 2 cut(s) 19, 1166
AcsI RAATTY 1 cut(s) 1093
AfaI GTAC 2 cut(s) 218, 812
AfiI CCNNNNNNNGG 1 cut(s) 317
AgsI TTSAA 5 cut(s) 419, 429, 473, 605, 1147
AjnI CCWGG 3 cut(s) 346, 832, 1134
AluBI AGCT 8 cut(s) 159, 183, 397, 521, 649, 677, 722, 820
AluI AGCT 8 cut(s) 159, 183, 397, 521, 649, 677, 722, 820
Alw21I GWGCWC 1 cut(s) 1025
Alw26I GTCTC 3 cut(s) 198, 304, 853
AlwI GGATC 4 cut(s) 792, 824, 837, 1110
AlwNI CAGNNNCTG 1 cut(s) 725
AoxI GGCC 3 cut(s) 19, 994, 1166
ApeKI GCWGC 6 cut(s) 93, 386, 527, 649, 652, 1212
ApoI RAATTY 1 cut(s) 1093
AspLEI GCGC 1 cut(s) 206
AspS9I GGNCC 2 cut(s) 308, 716
AsuC2I CCSGG 1 cut(s) 18
AvaII GGWCC 2 cut(s) 308, 716
BamHI GGATCC 1 cut(s) 829
BbsI GAAGAC 1 cut(s) 932
Bbv12I GWGCWC 1 cut(s) 1025
BbvI GCAGC 5 cut(s) 80, 373, 539, 636, 639
BccI CCATC 5 cut(s) 284, 376, 842, 986, 1106
BceAI ACGGC 2 cut(s) 1073, 1181
BciT130I CCWGG 3 cut(s) 348, 834, 1136
BclI TGATCA 1 cut(s) 114
BcnI CCSGG 1 cut(s) 18
BcoDI GTCTC 3 cut(s) 198, 304, 853
BfaI CTAG 4 cut(s) 180, 522, 788, 821
BfmI CTRYAG 2 cut(s) 94, 653
BfuAI ACCTGC 1 cut(s) 926
BisI GCNGC 7 cut(s) 43, 94, 387, 528, 650, 653, 1213
BlsI GCNGC 7 cut(s) 44, 95, 388, 529, 651, 654, 1214
BmcAI AGTACT 1 cut(s) 218
Bme1390I CCNGG 4 cut(s) 18, 348, 834, 1136
Bme18I GGWCC 2 cut(s) 308, 716
BmgT120I GGNCC 2 cut(s) 308, 716
BmiI GGNNCC 2 cut(s) 309, 831
BmrFI CCNGG 4 cut(s) 18, 348, 834, 1136
BmrI ACTGGG 1 cut(s) 492
BmsI GCATC 4 cut(s) 54, 199, 745, 1075
BmuI ACTGGG 1 cut(s) 492
BoxI GACNNNNGTC 2 cut(s) 438, 865
BpiI GAAGAC 1 cut(s) 932
BplI GAGNNNNNCTC 4 cut(s) 286, 318, 307, 339
BpmI CTGGAG 2 cut(s) 309, 1118
BpuMI CCSGG 1 cut(s) 18
BsaI GGTCTC 1 cut(s) 198
BsaXI ACNNNNNCTCC 2 cut(s) 573, 603
Bsc4I CCNNNNNNNGG 1 cut(s) 317
Bse118I RCCGGY 1 cut(s) 1074
Bse1I ACTGG 2 cut(s) 326, 487
Bse3DI GCAATG 2 cut(s) 22, 1146
BseBI CCWGG 3 cut(s) 348, 834, 1136
BseGI GGATG 5 cut(s) 45, 214, 235, 853, 997
BseLI CCNNNNNNNGG 1 cut(s) 317
BseMI GCAATG 2 cut(s) 22, 1146
BseMII CTCAG 3 cut(s) 204, 305, 317
BseNI ACTGG 2 cut(s) 326, 487
BseXI GCAGC 5 cut(s) 80, 373, 539, 636, 639
BsgI GTGCAG 2 cut(s) 546, 1158
Bsh1236I CGCG 1 cut(s) 206
BshFI GGCC 3 cut(s) 21, 996, 1168
BsiHKAI GWGCWC 1 cut(s) 1025
BsiSI CCGG 2 cut(s) 18, 1075
BslFI GGGAC 2 cut(s) 334, 755
BslI CCNNNNNNNGG 1 cut(s) 317
BsmAI GTCTC 3 cut(s) 198, 304, 853
BsmBI CGTCTC 1 cut(s) 853
BsmFI GGGAC 2 cut(s) 334, 755
BsmI GAATGC 2 cut(s) 881, 959
BsnI GGCC 3 cut(s) 21, 996, 1168
Bso31I GGTCTC 1 cut(s) 198
Bsp1286I GDGCHC 1 cut(s) 1025
Bsp143I GATC 6 cut(s) 114, 276, 688, 784, 829, 1102
BspACI CCGC 5 cut(s) 35, 43, 206, 238, 311
BspANI GGCC 3 cut(s) 21, 996, 1168
BspCNI CTCAG 3 cut(s) 205, 306, 316
BspFNI CGCG 1 cut(s) 206
BspHI TCATGA 2 cut(s) 168, 1200
BspLI GGNNCC 2 cut(s) 309, 831
BspMAI CTGCAG 2 cut(s) 98, 657
BspMI ACCTGC 1 cut(s) 926
BspPI GGATC 4 cut(s) 792, 824, 837, 1110
BspQI GCTCTTC 1 cut(s) 190
BspTNI GGTCTC 1 cut(s) 198
BsrDI GCAATG 2 cut(s) 22, 1146
BsrFI RCCGGY 1 cut(s) 1074
BsrI ACTGG 2 cut(s) 326, 487
BssAI RCCGGY 1 cut(s) 1074
BssMI GATC 6 cut(s) 114, 276, 688, 784, 829, 1102
Bst2UI CCWGG 3 cut(s) 348, 834, 1136
Bst4CI ACNGT 2 cut(s) 1061, 1207
Bst6I CTCTTC 1 cut(s) 190
BstAPI GCANNNNNTGC 1 cut(s) 536
BstC8I GCNNGC 1 cut(s) 498
BstDEI CTNAG 6 cut(s) 65, 213, 303, 314, 766, 955
BstF5I GGATG 5 cut(s) 45, 214, 235, 853, 997
BstFNI CGCG 1 cut(s) 206
BstHHI GCGC 1 cut(s) 206
BstKTI GATC 6 cut(s) 117, 279, 691, 787, 832, 1105
BstMAI GTCTC 3 cut(s) 198, 304, 853
BstMBI GATC 6 cut(s) 114, 276, 688, 784, 829, 1102
BstMWI GCNNNNNNNGC 4 cut(s) 527, 536, 872, 956
BstNI CCWGG 3 cut(s) 348, 834, 1136
BstNSI RCATGY 1 cut(s) 685
BstPAI GACNNNNGTC 2 cut(s) 438, 865
BstSCI CCNGG 4 cut(s) 16, 346, 832, 1134
BstSFI CTRYAG 2 cut(s) 94, 653
BstUI CGCG 1 cut(s) 206
BstV1I GCAGC 5 cut(s) 80, 373, 539, 636, 639
BstV2I GAAGAC 1 cut(s) 932
BstX2I RGATCY 3 cut(s) 784, 829, 1102
BstXI CCANNNNNNTGG 1 cut(s) 713
BstYI RGATCY 3 cut(s) 784, 829, 1102
BsuRI GGCC 3 cut(s) 21, 996, 1168
BtsCI GGATG 5 cut(s) 45, 214, 235, 853, 997
BtsI GCAGTG 2 cut(s) 274, 497
BtsIMutI CAGTG 2 cut(s) 274, 497
BveI ACCTGC 1 cut(s) 926
Cac8I GCNNGC 1 cut(s) 498
CaiI CAGNNNCTG 1 cut(s) 725
CciI TCATGA 2 cut(s) 168, 1200
CfoI GCGC 1 cut(s) 206
Cfr10I RCCGGY 1 cut(s) 1074
Cfr13I GGNCC 2 cut(s) 308, 716
CseI GACGC 1 cut(s) 855
Csp6I GTAC 2 cut(s) 217, 811
CviAII CATG 4 cut(s) 169, 682, 686, 1201
CviQI GTAC 2 cut(s) 217, 811
DdeI CTNAG 6 cut(s) 65, 213, 303, 314, 766, 955
DpnI GATC 6 cut(s) 116, 278, 690, 786, 831, 1104
DpnII GATC 6 cut(s) 114, 276, 688, 784, 829, 1102
EaeI YGGCCR 2 cut(s) 19, 1166
Eam1104I CTCTTC 1 cut(s) 190
EarI CTCTTC 1 cut(s) 190
Eco31I GGTCTC 1 cut(s) 198
Eco32I GATATC 1 cut(s) 1039
Eco47I GGWCC 2 cut(s) 308, 716
EcoRII CCWGG 3 cut(s) 346, 832, 1134
EcoRV GATATC 1 cut(s) 1039
EcoT22I ATGCAT 1 cut(s) 687
Esp3I CGTCTC 1 cut(s) 853
FaeI CATG 4 cut(s) 172, 685, 689, 1204
FaqI GGGAC 2 cut(s) 334, 755
FatI CATG 4 cut(s) 168, 681, 685, 1200
FbaI TGATCA 1 cut(s) 114
FblI GTMKAC 2 cut(s) 145, 869
Fnu4HI GCNGC 7 cut(s) 43, 94, 387, 528, 650, 653, 1213
FokI GGATG 5 cut(s) 32, 221, 242, 860, 1004
Fsp4HI GCNGC 7 cut(s) 43, 94, 387, 528, 650, 653, 1213
FspBI CTAG 4 cut(s) 180, 522, 788, 821
GlaI GCGC 1 cut(s) 205
GluI GCNGC 7 cut(s) 43, 94, 387, 528, 650, 653, 1213
GsuI CTGGAG 2 cut(s) 309, 1118
HaeIII GGCC 3 cut(s) 21, 996, 1168
HapII CCGG 2 cut(s) 18, 1075
HgaI GACGC 1 cut(s) 855
HhaI GCGC 1 cut(s) 206
Hin1II CATG 4 cut(s) 172, 685, 689, 1204
Hin6I GCGC 1 cut(s) 204
HinP1I GCGC 1 cut(s) 204
HincII GTYRAC 2 cut(s) 146, 802
HindII GTYRAC 2 cut(s) 146, 802
HpaII CCGG 2 cut(s) 18, 1075
Hpy166II GTNNAC 5 cut(s) 146, 763, 802, 813, 870
Hpy188III TCNNGA 7 cut(s) 118, 169, 692, 782, 1100, 1151, 1201
Hpy8I GTNNAC 5 cut(s) 146, 763, 802, 813, 870
HpyAV CCTTC 4 cut(s) 257, 425, 818, 916
HpyCH4III ACNGT 2 cut(s) 1061, 1207
HpyCH4V TGCA 8 cut(s) 96, 527, 655, 685, 703, 881, 1006, 1175
HpyF10VI GCNNNNNNNGC 4 cut(s) 527, 536, 872, 956
HpyF3I CTNAG 6 cut(s) 65, 213, 303, 314, 766, 955
Hsp92II CATG 4 cut(s) 172, 685, 689, 1204
HspAI GCGC 1 cut(s) 204
Ksp22I TGATCA 1 cut(s) 114
Kzo9I GATC 6 cut(s) 114, 276, 688, 784, 829, 1102
LguI GCTCTTC 1 cut(s) 190
LmnI GCTCC 4 cut(s) 9, 156, 487, 1043
Lsp1109I GCAGC 5 cut(s) 80, 373, 539, 636, 639
LweI GCATC 4 cut(s) 54, 199, 745, 1075
MaeI CTAG 4 cut(s) 180, 522, 788, 821
MaeIII GTNAC 3 cut(s) 453, 640, 1128
MalI GATC 6 cut(s) 116, 278, 690, 786, 831, 1104
MboI GATC 6 cut(s) 114, 276, 688, 784, 829, 1102
MboII GAAGA 3 cut(s) 177, 642, 932
MfeI CAATTG 1 cut(s) 741
MflI RGATCY 3 cut(s) 784, 829, 1102
MhlI GDGCHC 1 cut(s) 1025
MluCI AATT 4 cut(s) 424, 598, 741, 1093
MlyI GAGTC 6 cut(s) 76, 122, 255, 306, 316, 718
MmeI TCCRAC 1 cut(s) 1054
Mph1103I ATGCAT 1 cut(s) 687
MseI TTAA 2 cut(s) 336, 735
MslI CAYNNNNRTG 2 cut(s) 690, 890
MspA1I CMGCKG 1 cut(s) 313
MspI CCGG 2 cut(s) 18, 1075
MspR9I CCNGG 4 cut(s) 18, 348, 834, 1136
MunI CAATTG 1 cut(s) 741
Mva1269I GAATGC 2 cut(s) 881, 959
MvaI CCWGG 3 cut(s) 348, 834, 1136
MvnI CGCG 1 cut(s) 206
MwoI GCNNNNNNNGC 4 cut(s) 527, 536, 872, 956
NciI CCSGG 1 cut(s) 18
NdeII GATC 6 cut(s) 114, 276, 688, 784, 829, 1102
NlaIII CATG 4 cut(s) 172, 685, 689, 1204
NlaIV GGNNCC 2 cut(s) 309, 831
NsiI ATGCAT 1 cut(s) 687
NspI RCATGY 1 cut(s) 685
PagI TCATGA 2 cut(s) 168, 1200
PciSI GCTCTTC 1 cut(s) 190
PctI GAATGC 2 cut(s) 881, 959
PfeI GAWTC 4 cut(s) 109, 445, 1147, 1189
PfoI TCCNGGA 2 cut(s) 346, 832
PkrI GCNGC 7 cut(s) 44, 95, 388, 529, 651, 654, 1214
PleI GAGTC 6 cut(s) 76, 122, 254, 305, 316, 717
PpsI GAGTC 6 cut(s) 76, 122, 254, 305, 316, 717
PshAI GACNNNNGTC 2 cut(s) 438, 865
Psp6I CCWGG 3 cut(s) 346, 832, 1134
PspGI CCWGG 3 cut(s) 346, 832, 1134
PspN4I GGNNCC 2 cut(s) 309, 831
PspPI GGNCC 2 cut(s) 308, 716
PstI CTGCAG 2 cut(s) 98, 657
PstNI CAGNNNCTG 1 cut(s) 725
PsuI RGATCY 3 cut(s) 784, 829, 1102
RsaI GTAC 2 cut(s) 218, 812
RsaNI GTAC 2 cut(s) 217, 811
RseI CAYNNNNRTG 2 cut(s) 690, 890
SalI GTCGAC 1 cut(s) 144
SapI GCTCTTC 1 cut(s) 190
SaqAI TTAA 2 cut(s) 336, 735
SatI GCNGC 7 cut(s) 43, 94, 387, 528, 650, 653, 1213
Sau3AI GATC 6 cut(s) 114, 276, 688, 784, 829, 1102
Sau96I GGNCC 2 cut(s) 308, 716
ScaI AGTACT 1 cut(s) 218
SchI GAGTC 6 cut(s) 76, 122, 255, 306, 316, 718
ScrFI CCNGG 4 cut(s) 18, 348, 834, 1136
SduI GDGCHC 1 cut(s) 1025
SfaNI GCATC 4 cut(s) 54, 199, 745, 1075
SfcI CTRYAG 2 cut(s) 94, 653
SinI GGWCC 2 cut(s) 308, 716
SmiMI CAYNNNNRTG 2 cut(s) 690, 890
Sse9I AATT 4 cut(s) 424, 598, 741, 1093
SsiI CCGC 5 cut(s) 35, 43, 206, 238, 311
SspMI CTAG 4 cut(s) 180, 522, 788, 821
StyD4I CCNGG 4 cut(s) 16, 346, 832, 1134
TaaI ACNGT 2 cut(s) 1061, 1207
TaqI TCGA 3 cut(s) 135, 145, 775
TasI AATT 4 cut(s) 424, 598, 741, 1093
TatI WGTACW 1 cut(s) 216
TauI GCSGC 1 cut(s) 45
TfiI GAWTC 4 cut(s) 109, 445, 1147, 1189
Tru1I TTAA 2 cut(s) 336, 735
Tru9I TTAA 2 cut(s) 336, 735
TscAI CASTG 2 cut(s) 274, 497
TseI GCWGC 6 cut(s) 93, 386, 527, 649, 652, 1212
TspDTI ATGAA 3 cut(s) 157, 185, 1189
TspGWI ACGGA 1 cut(s) 594
TspRI CASTG 2 cut(s) 274, 497
VpaK11BI GGWCC 2 cut(s) 308, 716
XapI RAATTY 1 cut(s) 1093
XceI RCATGY 1 cut(s) 685
XmiI GTMKAC 2 cut(s) 145, 869
XspI CTAG 4 cut(s) 180, 522, 788, 821
ZrmI AGTACT 1 cut(s) 218
Zsp2I ATGCAT 1 cut(s) 687
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.