Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr2

Orthogroup ID

OG0011029

Physical Location & Seq

Genomic Coordinates Reverse (-)

61720632 .. 61726012

Gene Structure 5381 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000010010

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1281 bp

ATGCTGCTCGCGCCACCACCTGATTGGCTCCTTCCGGCTCGTCGAATTTACAGACCCAACTCACCACCACCGATCAGAACTCGGACTCGGACGACTCGGCTGTGCTGTAGAGCGAGTCTGATCACCAACTCGGACTCGTTCGAGGTGGGTCGACTCATTGGCAGCTATGGCTTCATGAACATCACTAGTAGAAAGATATGTTATATTCCCTCCTCATCCGGAGATTTGGGGTGGTTGAGGGTTCAAGATGTAGGAGAAGGCAGTGTAAAGATCAGGCTATATGATGGGAGAGTTACTCAAGGTCCGCTGAAAGGGGCTCCAGTTGTTTTTAAGGTTTATCCTGGAAAAAGGGCCGATGGAAATGAGGCTGATATGATGGCTGCTAATGAGCTAAATGCTCACACATCCCTTCAGGACAGTTCAGATGGTATCTGCCAGAATCTTGTGACACTCATAGGTGGGTTTGAACAAAAACTGGGGAGCAGGTTTTGTGCAGATTATGGAAAAGTTATGAGTGAAAGAGTTTCAAAAAGCCGTGCTGGGGGAGGACAGGTTTGGAATAAGTTTGAACAAGAGGAAACAATCAAACGCAGAAGATACTATGTGACCAAGTTGCTTCAGGGTATGATGAGAGGTCTAGCATACATGCATGATCGTGAAAGGTTACACCAGAGTCTGGGACCAGCTTCTGTCATTCTTAACACAATTGTAGAGAGAGAGGCCATATACTTAGTACCACGACTCCGGGAGCTAGCCTTTTCTGTTGACATTAGGTATTCAAATCTAGAAGGGAGGCCTGGACTACTGTCGGAGGGACTTTGGAGACGAGCATCTACCGCTGGTGCCTTCACACCTATGGATAAAAGAGCATTTGGACTGGCAGATGACATATATGAAGCAGGTCTTTTCTTTGCATACTTGGCATTTGTTCCATTTTGTGAAGCAGGCACAATGGATGGTCTTTCGTTGCAAAGGATTTTGGAGAGCACTTTCCAACTTGATCTTCGAGCAACAAGAGAATACTGTTTAGCAGATGACAGGCTTTTAGATGCTGTGAAATTTCTGGATCTTGGAGATGGTGCTGGTTGGGAGTTACTCCAGGCAATGCTGAATCCCGACTTCCCGGAAACGGCCGATGGCACAGACTGTACTCAATCATCGATTCATGACCGTGAGTGTTCTTTGAGATTTTGGACCGCAGAATTTGATCTATTGTGTATGTTGTATGATAGACATGGATCATCAAGTGAACTATGTTTGGGTGATATTTCTTTGCAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase

Protein Analysis

427

Amino Acids

47.7

Weight (kDa)

6.28

Isoelectric Point (pI)

46.65

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011029)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G16810 AT5G16810
fragaria_vesca	FvH4_4g01950
malus_domestica	MD13G1202900.v1.1
prunus_persica	Prupe.1G025200_v2.0.a1 Prupe.1G025200_v2.0.a1 Prupe.1G025200_v2.0.a1
pyrus_communis	pycom13g17620
rosa_chinensis	RchiOBHm_Chr4g0388921
rosa_laevigata	RLG00000010007 RLG00000010010
rosa_multiflora	Rmu_sc0000697.1_g000001 Rmu_sc0005486.1_g000008
rosa_roxburghii	Rroxscaffold_5G00335230
rosa_rugosa	Rorug03G0321200
rosa_samantha	Rh4AG025700 Rh4BG018900 Rh4CG028800 Rh4DG019400
rosa_wichuraiana	Rw4G001850

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	474, 890
AccB1I	GGYRCC	1 cut(s)	842
AccB7I	CCANNNNNTGG	1 cut(s)	676
AccI	GTMKAC	1 cut(s)	151
AccII	CGCG	1 cut(s)	11
AccIII	TCCGGA	1 cut(s)	218
AciI	CCGC	3 cut(s)	305, 837, 1197
AclWI	GGATC	2 cut(s)	1074, 1246
AcoI	YGGCCR	1 cut(s)	1131
AcsI	RAATTY	3 cut(s)	45, 1058, 1202
AcuI	CTGAAG	2 cut(s)	395, 602
AfaI	GTAC	2 cut(s)	735, 1150
AfiI	CCNNNNNNNGG	4 cut(s)	311, 541, 676, 1129
AgsI	TTSAA	5 cut(s)	245, 467, 528, 569, 780
AhlI	ACTAGT	1 cut(s)	185
AjnI	CCWGG	3 cut(s)	340, 796, 1098
AjuI	GAANNNNNNNTTGG	2 cut(s)	987, 1019
AluBI	AGCT	4 cut(s)	165, 391, 686, 751
AluI	AGCT	4 cut(s)	165, 391, 686, 751
Alw21I	GWGCWC	1 cut(s)	989
Alw26I	GTCTC	1 cut(s)	817
AlwI	GGATC	2 cut(s)	1074, 1246
AlwNI	CAGNNNCTG	2 cut(s)	676, 689
Aor13HI	TCCGGA	1 cut(s)	218
AoxI	GGCC	4 cut(s)	351, 720, 794, 1131
ApeKI	GCWGC	3 cut(s)	4, 162, 380
ApoI	RAATTY	3 cut(s)	45, 1058, 1202
AspLEI	GCGC	1 cut(s)	13
AspS9I	GGNCC	4 cut(s)	302, 351, 680, 1194
AsuC2I	CCSGG	2 cut(s)	746, 1124
AsuHPI	GGTGA	3 cut(s)	54, 115, 1274
AsuNHI	GCTAGC	1 cut(s)	751
AvaII	GGWCC	3 cut(s)	302, 680, 1194
BanI	GGYRCC	1 cut(s)	842
BanII	GRGCYC	1 cut(s)	319
Bbv12I	GWGCWC	1 cut(s)	989
BbvI	GCAGC	2 cut(s)	174, 367
BccI	CCATC	7 cut(s)	278, 350, 370, 419, 950, 1070, 1130
BceAI	ACGGC	2 cut(s)	519, 1146
BciT130I	CCWGG	3 cut(s)	342, 798, 1100
BclI	TGATCA	1 cut(s)	120
BcnI	CCSGG	2 cut(s)	746, 1124
BcoDI	GTCTC	1 cut(s)	817
BcuI	ACTAGT	1 cut(s)	185
BfaI	CTAG	4 cut(s)	186, 638, 752, 785
BfmI	CTRYAG	1 cut(s)	106
BfuAI	ACCTGC	2 cut(s)	474, 890
BisI	GCNGC	3 cut(s)	5, 163, 381
BlsI	GCNGC	3 cut(s)	6, 164, 382
Bme1390I	CCNGG	5 cut(s)	342, 746, 798, 1100, 1124
Bme18I	GGWCC	3 cut(s)	302, 680, 1194
BmgT120I	GGNCC	4 cut(s)	302, 351, 680, 1194
BmiI	GGNNCC	4 cut(s)	29, 318, 681, 844
BmrFI	CCNGG	5 cut(s)	342, 746, 798, 1100, 1124
BmrI	ACTGGG	1 cut(s)	485
BmsI	GCATC	2 cut(s)	839, 1039
BmtI	GCTAGC	1 cut(s)	755
BmuI	ACTGGG	1 cut(s)	485
BoxI	GACNNNNGTC	1 cut(s)	805
BplI	GAGNNNNNCTC	2 cut(s)	280, 312
BpmI	CTGGAG	2 cut(s)	303, 1082
BpuEI	CTTGAG	1 cut(s)	282
BpuMI	CCSGG	2 cut(s)	746, 1124
Bsa29I	ATCGAT	1 cut(s)	1160
BsaWI	WCCGGW	1 cut(s)	218
BsaXI	ACNNNNNCTCC	4 cut(s)	537, 567, 726, 756
Bsc4I	CCNNNNNNNGG	4 cut(s)	311, 541, 676, 1129
Bse1I	ACTGG	3 cut(s)	320, 480, 882
Bse3DI	GCAATG	1 cut(s)	1110
BseAI	TCCGGA	1 cut(s)	218
BseBI	CCWGG	3 cut(s)	342, 798, 1100
BseCI	ATCGAT	1 cut(s)	1160
BseGI	GGATG	3 cut(s)	215, 404, 961
BseLI	CCNNNNNNNGG	4 cut(s)	311, 541, 676, 1129
BseMI	GCAATG	1 cut(s)	1110
BseNI	ACTGG	3 cut(s)	320, 480, 882
BseRI	GAGGAG	1 cut(s)	202
BseX3I	CGGCCG	1 cut(s)	1131
BseXI	GCAGC	2 cut(s)	174, 367
BseYI	CCCAGC	1 cut(s)	539
BsgI	GTGCAG	1 cut(s)	513
Bsh1236I	CGCG	1 cut(s)	11
Bsh1285I	CGRYCG	1 cut(s)	1134
BshFI	GGCC	4 cut(s)	353, 722, 796, 1133
BshNI	GGYRCC	1 cut(s)	842
BshVI	ATCGAT	1 cut(s)	1160
BsiEI	CGRYCG	1 cut(s)	1134
BsiHKAI	GWGCWC	1 cut(s)	989
BsiSI	CCGG	4 cut(s)	35, 219, 745, 1124
BslFI	GGGAC	2 cut(s)	693, 828
BslI	CCNNNNNNNGG	4 cut(s)	311, 541, 676, 1129
BsmAI	GTCTC	1 cut(s)	817
BsmBI	CGTCTC	1 cut(s)	817
BsmFI	GGGAC	2 cut(s)	693, 828
BsnI	GGCC	4 cut(s)	353, 722, 796, 1133
Bsp1286I	GDGCHC	2 cut(s)	319, 989
Bsp13I	TCCGGA	1 cut(s)	218
Bsp143I	GATC	8 cut(s)	72, 120, 270, 652, 1000, 1066, 1207, 1238
BspACI	CCGC	3 cut(s)	305, 837, 1197
BspANI	GGCC	4 cut(s)	353, 722, 796, 1133
BspDI	ATCGAT	1 cut(s)	1160
BspEI	TCCGGA	1 cut(s)	218
BspFNI	CGCG	1 cut(s)	11
BspHI	TCATGA	2 cut(s)	174, 1165
BspLI	GGNNCC	4 cut(s)	29, 318, 681, 844
BspMI	ACCTGC	2 cut(s)	474, 890
BspOI	GCTAGC	1 cut(s)	755
BspPI	GGATC	2 cut(s)	1074, 1246
BspT107I	GGYRCC	1 cut(s)	842
BsrDI	GCAATG	1 cut(s)	1110
BsrI	ACTGG	3 cut(s)	320, 480, 882
BssMI	GATC	8 cut(s)	72, 120, 270, 652, 1000, 1066, 1207, 1238
Bst2UI	CCWGG	3 cut(s)	342, 798, 1100
Bst4CI	ACNGT	5 cut(s)	419, 807, 1025, 1148, 1172
BstC8I	GCNNGC	3 cut(s)	9, 753, 946
BstDEI	CTNAG	1 cut(s)	730
BstF5I	GGATG	3 cut(s)	215, 404, 961
BstFNI	CGCG	1 cut(s)	11
BstHHI	GCGC	1 cut(s)	13
BstKTI	GATC	8 cut(s)	75, 123, 273, 655, 1003, 1069, 1210, 1241
BstMAI	GTCTC	1 cut(s)	817
BstMBI	GATC	8 cut(s)	72, 120, 270, 652, 1000, 1066, 1207, 1238
BstMCI	CGRYCG	1 cut(s)	1134
BstMWI	GCNNNNNNNGC	4 cut(s)	10, 168, 836, 920
BstNI	CCWGG	3 cut(s)	342, 798, 1100
BstNSI	RCATGY	1 cut(s)	649
BstPAI	GACNNNNGTC	1 cut(s)	805
BstSCI	CCNGG	5 cut(s)	340, 744, 796, 1098, 1122
BstSFI	CTRYAG	1 cut(s)	106
BstUI	CGCG	1 cut(s)	11
BstV1I	GCAGC	2 cut(s)	174, 367
BstX2I	RGATCY	1 cut(s)	1066
BstXI	CCANNNNNNTGG	1 cut(s)	24
BstYI	RGATCY	1 cut(s)	1066
BstZI	CGGCCG	1 cut(s)	1131
Bsu15I	ATCGAT	1 cut(s)	1160
BsuRI	GGCC	4 cut(s)	353, 722, 796, 1133
BsuTUI	ATCGAT	1 cut(s)	1160
BtsCI	GGATG	3 cut(s)	215, 404, 961
BtsI	GCAGTG	1 cut(s)	268
BtsIMutI	CAGTG	1 cut(s)	268
BveI	ACCTGC	2 cut(s)	474, 890
Cac8I	GCNNGC	3 cut(s)	9, 753, 946
CaiI	CAGNNNCTG	2 cut(s)	676, 689
CciI	TCATGA	2 cut(s)	174, 1165
CfoI	GCGC	1 cut(s)	13
Cfr13I	GGNCC	4 cut(s)	302, 351, 680, 1194
ClaI	ATCGAT	1 cut(s)	1160
Csp6I	GTAC	2 cut(s)	734, 1149
CviAII	CATG	5 cut(s)	175, 646, 650, 1166, 1235
CviQI	GTAC	2 cut(s)	734, 1149
DdeI	CTNAG	1 cut(s)	730
DpnI	GATC	8 cut(s)	74, 122, 272, 654, 1002, 1068, 1209, 1240
DpnII	GATC	8 cut(s)	72, 120, 270, 652, 1000, 1066, 1207, 1238
EaeI	YGGCCR	1 cut(s)	1131
EagI	CGGCCG	1 cut(s)	1131
EclXI	CGGCCG	1 cut(s)	1131
Eco147I	AGGCCT	1 cut(s)	796
Eco24I	GRGCYC	1 cut(s)	319
Eco47I	GGWCC	3 cut(s)	302, 680, 1194
Eco52I	CGGCCG	1 cut(s)	1131
Eco57I	CTGAAG	2 cut(s)	395, 602
EcoRII	CCWGG	3 cut(s)	340, 796, 1098
EcoT22I	ATGCAT	1 cut(s)	651
EcoT38I	GRGCYC	1 cut(s)	319
Esp3I	CGTCTC	1 cut(s)	817
FaeI	CATG	5 cut(s)	178, 649, 653, 1169, 1238
FalI	AAGNNNNNCTT	2 cut(s)	888, 920
FaqI	GGGAC	2 cut(s)	693, 828
FatI	CATG	5 cut(s)	174, 645, 649, 1165, 1234
FbaI	TGATCA	1 cut(s)	120
FblI	GTMKAC	1 cut(s)	151
Fnu4HI	GCNGC	3 cut(s)	5, 163, 381
FokI	GGATG	3 cut(s)	202, 391, 968
FriOI	GRGCYC	1 cut(s)	319
Fsp4HI	GCNGC	3 cut(s)	5, 163, 381
FspBI	CTAG	4 cut(s)	186, 638, 752, 785
GlaI	GCGC	1 cut(s)	12
GluI	GCNGC	3 cut(s)	5, 163, 381
GsaI	CCCAGC	1 cut(s)	543
GsuI	CTGGAG	2 cut(s)	303, 1082
HaeIII	GGCC	4 cut(s)	353, 722, 796, 1133
HapII	CCGG	4 cut(s)	35, 219, 745, 1124
HhaI	GCGC	1 cut(s)	13
Hin1II	CATG	5 cut(s)	178, 649, 653, 1169, 1238
Hin6I	GCGC	1 cut(s)	11
HinP1I	GCGC	1 cut(s)	11
HincII	GTYRAC	2 cut(s)	152, 766
HindII	GTYRAC	2 cut(s)	152, 766
HpaII	CCGG	4 cut(s)	35, 219, 745, 1124
HphI	GGTGA	3 cut(s)	54, 115, 1274
Hpy166II	GTNNAC	3 cut(s)	152, 766, 1250
Hpy188I	TCNGA	7 cut(s)	77, 84, 90, 120, 133, 424, 811
Hpy188III	TCNNGA	9 cut(s)	175, 219, 245, 413, 656, 785, 1064, 1115, 1166
Hpy8I	GTNNAC	3 cut(s)	152, 766, 1250
Hpy99I	CGWCG	1 cut(s)	45
HpyAV	CCTTC	5 cut(s)	41, 251, 419, 782, 856
HpyCH4III	ACNGT	5 cut(s)	419, 807, 1025, 1148, 1172
HpyCH4V	TGCA	5 cut(s)	494, 649, 914, 970, 1276
HpyF10VI	GCNNNNNNNGC	4 cut(s)	10, 168, 836, 920
HpyF3I	CTNAG	1 cut(s)	730
Hsp92II	CATG	5 cut(s)	178, 649, 653, 1169, 1238
HspAI	GCGC	1 cut(s)	11
Kpn2I	TCCGGA	1 cut(s)	218
Ksp22I	TGATCA	1 cut(s)	120
Kzo9I	GATC	8 cut(s)	72, 120, 270, 652, 1000, 1066, 1207, 1238
LmnI	GCTCC	4 cut(s)	33, 322, 480, 748
Lsp1109I	GCAGC	2 cut(s)	174, 367
LweI	GCATC	2 cut(s)	839, 1039
MaeI	CTAG	4 cut(s)	186, 638, 752, 785
MaeIII	GTNAC	5 cut(s)	292, 445, 604, 663, 1092
MalI	GATC	8 cut(s)	74, 122, 272, 654, 1002, 1068, 1209, 1240
MboI	GATC	8 cut(s)	72, 120, 270, 652, 1000, 1066, 1207, 1238
MboII	GAAGA	2 cut(s)	606, 995
MfeI	CAATTG	1 cut(s)	705
MflI	RGATCY	1 cut(s)	1066
MhlI	GDGCHC	2 cut(s)	319, 989
MluCI	AATT	4 cut(s)	45, 705, 1058, 1202
MlyI	GAGTC	7 cut(s)	79, 88, 124, 128, 147, 682, 735
MmeI	TCCRAC	2 cut(s)	789, 1018
Mph1103I	ATGCAT	1 cut(s)	651
MroI	TCCGGA	1 cut(s)	218
MseI	TTAA	2 cut(s)	330, 699
MslI	CAYNNNNRTG	3 cut(s)	654, 854, 1170
MspA1I	CMGCKG	2 cut(s)	307, 839
MspI	CCGG	4 cut(s)	35, 219, 745, 1124
MspR9I	CCNGG	5 cut(s)	342, 746, 798, 1100, 1124
MunI	CAATTG	1 cut(s)	705
MvaI	CCWGG	3 cut(s)	342, 798, 1100
MvnI	CGCG	1 cut(s)	11
MwoI	GCNNNNNNNGC	4 cut(s)	10, 168, 836, 920
NciI	CCSGG	2 cut(s)	746, 1124
NdeII	GATC	8 cut(s)	72, 120, 270, 652, 1000, 1066, 1207, 1238
NheI	GCTAGC	1 cut(s)	751
NlaIII	CATG	5 cut(s)	178, 649, 653, 1169, 1238
NlaIV	GGNNCC	4 cut(s)	29, 318, 681, 844
NmeAIII	GCCGAG	1 cut(s)	76
NmuCI	GTSAC	2 cut(s)	445, 604
NsiI	ATGCAT	1 cut(s)	651
NspI	RCATGY	1 cut(s)	649
PagI	TCATGA	2 cut(s)	174, 1165
PceI	AGGCCT	1 cut(s)	796
PfeI	GAWTC	3 cut(s)	439, 1111, 1162
PflMI	CCANNNNNTGG	1 cut(s)	676
PfoI	TCCNGGA	3 cut(s)	340, 744, 1122
PkrI	GCNGC	3 cut(s)	6, 164, 382
PleI	GAGTC	7 cut(s)	79, 88, 123, 128, 147, 681, 735
PpsI	GAGTC	7 cut(s)	79, 88, 123, 128, 147, 681, 735
PshAI	GACNNNNGTC	1 cut(s)	805
Psp6I	CCWGG	3 cut(s)	340, 796, 1098
PspFI	CCCAGC	1 cut(s)	539
PspGI	CCWGG	3 cut(s)	340, 796, 1098
PspN4I	GGNNCC	4 cut(s)	29, 318, 681, 844
PspPI	GGNCC	4 cut(s)	302, 351, 680, 1194
PstNI	CAGNNNCTG	2 cut(s)	676, 689
PsuI	RGATCY	1 cut(s)	1066
RsaI	GTAC	2 cut(s)	735, 1150
RsaNI	GTAC	2 cut(s)	734, 1149
RseI	CAYNNNNRTG	3 cut(s)	654, 854, 1170
SalI	GTCGAC	1 cut(s)	150
SaqAI	TTAA	2 cut(s)	330, 699
SatI	GCNGC	3 cut(s)	5, 163, 381
Sau3AI	GATC	8 cut(s)	72, 120, 270, 652, 1000, 1066, 1207, 1238
Sau96I	GGNCC	4 cut(s)	302, 351, 680, 1194
SchI	GAGTC	7 cut(s)	79, 88, 124, 128, 147, 682, 735
ScrFI	CCNGG	5 cut(s)	342, 746, 798, 1100, 1124
SduI	GDGCHC	2 cut(s)	319, 989
SfaNI	GCATC	2 cut(s)	839, 1039
SfcI	CTRYAG	1 cut(s)	106
SinI	GGWCC	3 cut(s)	302, 680, 1194
SmiMI	CAYNNNNRTG	3 cut(s)	654, 854, 1170
SmlI	CTYRAG	1 cut(s)	297
SmoI	CTYRAG	1 cut(s)	297
SpeI	ACTAGT	1 cut(s)	185
Sse9I	AATT	4 cut(s)	45, 705, 1058, 1202
SseBI	AGGCCT	1 cut(s)	796
SsiI	CCGC	3 cut(s)	305, 837, 1197
SspMI	CTAG	4 cut(s)	186, 638, 752, 785
StuI	AGGCCT	1 cut(s)	796
StyD4I	CCNGG	5 cut(s)	340, 744, 796, 1098, 1122
TaaI	ACNGT	5 cut(s)	419, 807, 1025, 1148, 1172
TaqI	TCGA	5 cut(s)	43, 141, 151, 1006, 1160
TasI	AATT	4 cut(s)	45, 705, 1058, 1202
TatI	WGTACW	1 cut(s)	1148
TfiI	GAWTC	3 cut(s)	439, 1111, 1162
Tru1I	TTAA	2 cut(s)	330, 699
Tru9I	TTAA	2 cut(s)	330, 699
TscAI	CASTG	1 cut(s)	268
TseFI	GTSAC	2 cut(s)	445, 604
TseI	GCWGC	3 cut(s)	4, 162, 380
Tsp45I	GTSAC	2 cut(s)	445, 604
TspDTI	ATGAA	4 cut(s)	163, 191, 909, 1154
TspRI	CASTG	1 cut(s)	268
Van91I	CCANNNNNTGG	1 cut(s)	676
VpaK11BI	GGWCC	3 cut(s)	302, 680, 1194
XapI	RAATTY	3 cut(s)	45, 1058, 1202
XbaI	TCTAGA	1 cut(s)	784
XceI	RCATGY	1 cut(s)	649
XcmI	CCANNNNNNNNNTGG	1 cut(s)	21
XmiI	GTMKAC	1 cut(s)	151
XspI	CTAG	4 cut(s)	186, 638, 752, 785
Zsp2I	ATGCAT	1 cut(s)	651

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.