Rorug03G0321200

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0011029
Physical Location & Seq
Forward (+)
34802430 .. 34803980
1551 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0321200.1

Sequence Viewer

Length: 1551 bp
ATGTGGCTCAATCATCCTAACTTTATTCAACTAGTTGATGGGTTTTGGAATGCTCTGCAATTTCATGGTTGCCCACTGTTCATACTAAGCTCTAAGTTGCGTGCTTTGAAGATTATGCTCAAGAGTTGGAATAAGACACACTTTGGGAACATTCATGCAAGAGTGAACATGGCAAGACAGGCCCTCGATATTGTTCAGGCAAAAATTAATTTGATGGGGCCTTCTGAGGATCGGTTTTTAAGGGAAGATGAAGCTCTTGCCACATTCCAGTCTGAGCTGTCCATGCATTATACCCTACTTAGAGAAAAGTCAAGGATTAGGTGGCTAAAGGATGGGGATCGCAACACTTCCTTTCTTCATAACATGGTTAAAGTCCGGAGTCTAAACAAGTCCATTACCTCGTTTACAGGACAAACCACTTTGCAGGATCAAGAGTCTATTGCGAACTACATTGTGCAACATTACACGAACTTGTTTTCTAGGGATCACAACATTATTGATTCTGGGTTGGTGGAGAGGACCATCCCTCACTTGGTTACAGAAGATGAGAATTTCTCTCTAGTTCGTATTCCCAGTGATGAGGAGGTAGCACAGGCAGTTCATCCCATGGATAGTAGTAGCTCACCTGGCCCGGATGGTTTTGGGGGAGAATTTTTTAAGGCTTGCTGGTCTGTGGTCTCTAGGGATGTGATTGCTGCTGTGCGTAGCTTCTTTGAGTTGGGCTTTATTCCCCCTCACTTTAATTCTAATCTGATCATTCTAATTCCGAAGAAGTTGGAACCAAAGAGTATATCTGATTACAGGCCAATAGCTTTGGCTAACTTTGTTTTTAAGGTTATCACCAAGATATTGGCGGATCGCTTGGGTCTGGATGCCTCGAGGATTGTCTCCCCCAACCAGAGTGCTTTCATACGAGGTAGATCGATTGTCGACCCGATTACCCTTACTTCAGAGTGTTTGAATCTTCTTGATCATAAGTGCAGGTCCGGAAATATTGCAATTAAATTTGATATTGCCAAAGCCTTTGATACTATTGATTGGAGCTTCCTTCTACGGGTTCTCCGAGATTTTGGCTTCAACCCAGGGTTTGTGAACTGGATTCAGAATATCCTTGCTTCAGCTCATCTTTCTGTATCAGTCAATGGTCAATCTTGTGGCTTCTTTACCTGCTCACGGGGTGTGCGTCAGGGAGACCCCTTATCACCTATTCTTTTCTGTCTTGCAGAGGAGGTTCTTAGTAGGGGTATCTCACAGCTCGTATCTCAAAATAATCTAGATTTCATGGCAGCTCCAGGATCTTGCTCAACCCCATCTCATGTTTTGTTTGCAGAAGACGTAATGGTTTTCATGCATGCTAGCTCTCATGGCCTCCATGCTATTTCAGATTTTATGGAGGAGTACGCAGCCAATTCAGGGCAGTATGTTAACAAGGAAAAATCTATCCTTTTTCTTGGACGGTATGCAATCTCAAGGTCTTCCACTATTCAAAACATTTTGGGTGTCCAATTGGGGTCGTTACCTTTCATGTATCTGGGGGTTCCCATCTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase

Protein Analysis

516

Amino Acids

57.88

Weight (kDa)

7.28

Isoelectric Point (pI)

51.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RVT_1 PF00078 259 - 515 6.2e-33 Reverse transcriptase (RNA-dependent DNA polymerase)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 877
Acc36I ACCTGC 2 cut(s) 972, 1175
AccI GTMKAC 1 cut(s) 930
AccIII TCCGGA 2 cut(s) 375, 986
AciI CCGC 1 cut(s) 854
AclWI GGATC 6 cut(s) 237, 345, 435, 492, 864, 1303
AcsI RAATTY 3 cut(s) 550, 650, 1004
AcuI CTGAAG 2 cut(s) 933, 1101
AdeI CACNNNGTG 1 cut(s) 1178
AfaI GTAC 1 cut(s) 1400
AfiI CCNNNNNNNGG 4 cut(s) 532, 1054, 1173, 1413
AgsI TTSAA 5 cut(s) 29, 109, 961, 1078, 1487
AhlI ACTAGT 1 cut(s) 31
AjnI CCWGG 3 cut(s) 625, 1081, 1291
Alw26I GTCTC 3 cut(s) 682, 892, 1185
AlwI GGATC 6 cut(s) 237, 345, 435, 492, 864, 1303
Ama87I CYCGRG 1 cut(s) 877
Aor13HI TCCGGA 2 cut(s) 375, 986
AoxI GGCC 5 cut(s) 180, 218, 628, 803, 1366
ApeKI GCWGC 3 cut(s) 695, 1286, 1403
ApoI RAATTY 3 cut(s) 550, 650, 1004
AseI ATTAAT 1 cut(s) 207
AspS9I GGNCC 5 cut(s) 181, 218, 519, 629, 984
AsuC2I CCSGG 1 cut(s) 632
AsuHPI GGTGA 3 cut(s) 615, 832, 1194
AsuNHI GCTAGC 1 cut(s) 1355
AvaI CYCGRG 1 cut(s) 877
AvaII GGWCC 2 cut(s) 519, 984
BbsI GAAGAC 2 cut(s) 1338, 1467
BbvI GCAGC 3 cut(s) 682, 1298, 1415
BccI CCATC 7 cut(s) 32, 208, 326, 530, 629, 1318, 1550
BciT130I CCWGG 3 cut(s) 627, 1083, 1293
BclI TGATCA 2 cut(s) 753, 970
BcnI CCSGG 1 cut(s) 632
BcoDI GTCTC 3 cut(s) 682, 892, 1185
BcuI ACTAGT 1 cut(s) 31
BfaI CTAG 6 cut(s) 32, 480, 560, 681, 1274, 1356
BfuAI ACCTGC 2 cut(s) 972, 1175
BisI GCNGC 3 cut(s) 696, 1287, 1404
BlsI GCNGC 3 cut(s) 697, 1288, 1405
Bme1390I CCNGG 4 cut(s) 627, 632, 1083, 1293
Bme18I GGWCC 2 cut(s) 519, 984
BmeT110I CYCGRG 1 cut(s) 877
BmgT120I GGNCC 5 cut(s) 181, 218, 519, 629, 984
BmiI GGNNCC 3 cut(s) 219, 780, 1539
BmrFI CCNGG 4 cut(s) 627, 632, 1083, 1293
BmrI ACTGGG 1 cut(s) 567
BmsI GCATC 1 cut(s) 862
BmtI GCTAGC 1 cut(s) 1359
BmuI ACTGGG 1 cut(s) 567
BpiI GAAGAC 2 cut(s) 1338, 1467
BplI GAGNNNNNCTC 2 cut(s) 539, 571
BpmI CTGGAG 1 cut(s) 1275
BpuEI CTTGAG 2 cut(s) 104, 1453
BpuMI CCSGG 1 cut(s) 632
Bsa29I ATCGAT 1 cut(s) 923
BsaBI GATNNNNATC 1 cut(s) 336
BsaI GGTCTC 2 cut(s) 682, 1185
BsaJI CCNNGG 3 cut(s) 606, 1081, 1082
BsaWI WCCGGW 2 cut(s) 375, 986
BsaXI ACNNNNNCTCC 2 cut(s) 1044, 1074
Bsc4I CCNNNNNNNGG 4 cut(s) 532, 1054, 1173, 1413
Bse1I ACTGG 3 cut(s) 268, 573, 1100
Bse8I GATNNNNATC 1 cut(s) 336
BseAI TCCGGA 2 cut(s) 375, 986
BseBI CCWGG 3 cut(s) 627, 1083, 1293
BseCI ATCGAT 1 cut(s) 923
BseDI CCNNGG 3 cut(s) 606, 1081, 1082
BseGI GGATG 7 cut(s) 13, 337, 522, 601, 640, 691, 877
BseJI GATNNNNATC 1 cut(s) 336
BseLI CCNNNNNNNGG 4 cut(s) 532, 1054, 1173, 1413
BseMII CTCAG 2 cut(s) 216, 264
BseNI ACTGG 3 cut(s) 268, 573, 1100
BseRI GAGGAG 3 cut(s) 596, 1241, 1409
BseXI GCAGC 3 cut(s) 682, 1298, 1415
BsgI GTGCAG 1 cut(s) 1000
BshFI GGCC 5 cut(s) 182, 220, 630, 805, 1368
BshVI ATCGAT 1 cut(s) 923
BsiHKCI CYCGRG 1 cut(s) 877
BsiSI CCGG 3 cut(s) 376, 632, 987
BslI CCNNNNNNNGG 4 cut(s) 532, 1054, 1173, 1413
BsmAI GTCTC 3 cut(s) 682, 892, 1185
BsmI GAATGC 1 cut(s) 55
BsnI GGCC 5 cut(s) 182, 220, 630, 805, 1368
Bso31I GGTCTC 2 cut(s) 682, 1185
BsoBI CYCGRG 1 cut(s) 877
Bsp13I TCCGGA 2 cut(s) 375, 986
Bsp143I GATC 9 cut(s) 229, 337, 427, 484, 753, 856, 920, 970, 1295
Bsp19I CCATGG 1 cut(s) 606
BspACI CCGC 1 cut(s) 854
BspANI GGCC 5 cut(s) 182, 220, 630, 805, 1368
BspCNI CTCAG 2 cut(s) 217, 265
BspDI ATCGAT 1 cut(s) 923
BspEI TCCGGA 2 cut(s) 375, 986
BspLI GGNNCC 3 cut(s) 219, 780, 1539
BspMI ACCTGC 2 cut(s) 972, 1175
BspOI GCTAGC 1 cut(s) 1359
BspPI GGATC 6 cut(s) 237, 345, 435, 492, 864, 1303
BspTNI GGTCTC 2 cut(s) 682, 1185
BsrI ACTGG 3 cut(s) 268, 573, 1100
BssECI CCNNGG 3 cut(s) 606, 1081, 1082
BssMI GATC 9 cut(s) 229, 337, 427, 484, 753, 856, 920, 970, 1295
BssT1I CCWWGG 1 cut(s) 606
Bst2UI CCWGG 3 cut(s) 627, 1083, 1293
Bst4CI ACNGT 2 cut(s) 78, 1458
BstC8I GCNNGC 4 cut(s) 102, 664, 1353, 1357
BstDEI CTNAG 6 cut(s) 86, 93, 225, 273, 299, 1235
BstDSI CCRYGG 1 cut(s) 606
BstF5I GGATG 7 cut(s) 13, 337, 522, 601, 640, 691, 877
BstKTI GATC 9 cut(s) 232, 340, 430, 487, 756, 859, 923, 973, 1298
BstMAI GTCTC 3 cut(s) 682, 892, 1185
BstMBI GATC 9 cut(s) 229, 337, 427, 484, 753, 856, 920, 970, 1295
BstMWI GCNNNNNNNGC 4 cut(s) 179, 283, 627, 1365
BstNI CCWGG 3 cut(s) 627, 1083, 1293
BstNSI RCATGY 1 cut(s) 1355
BstSCI CCNGG 4 cut(s) 625, 630, 1081, 1291
BstV1I GCAGC 3 cut(s) 682, 1298, 1415
BstV2I GAAGAC 2 cut(s) 1338, 1467
BstX2I RGATCY 1 cut(s) 1295
BstXI CCANNNNNNTGG 1 cut(s) 850
BstYI RGATCY 1 cut(s) 1295
Bsu15I ATCGAT 1 cut(s) 923
BsuRI GGCC 5 cut(s) 182, 220, 630, 805, 1368
BsuTUI ATCGAT 1 cut(s) 923
BtgI CCRYGG 1 cut(s) 606
BtsCI GGATG 7 cut(s) 13, 337, 522, 601, 640, 691, 877
BtsIMutI CAGTG 2 cut(s) 74, 580
BveI ACCTGC 2 cut(s) 972, 1175
Cac8I GCNNGC 4 cut(s) 102, 664, 1353, 1357
Cfr13I GGNCC 5 cut(s) 181, 218, 519, 629, 984
ClaI ATCGAT 1 cut(s) 923
CseI GACGC 1 cut(s) 1172
Csp6I GTAC 1 cut(s) 1399
CviQI GTAC 1 cut(s) 1399
DdeI CTNAG 6 cut(s) 86, 93, 225, 273, 299, 1235
DpnI GATC 9 cut(s) 231, 339, 429, 486, 755, 858, 922, 972, 1297
DpnII GATC 9 cut(s) 229, 337, 427, 484, 753, 856, 920, 970, 1295
DraIII CACNNNGTG 1 cut(s) 1178
EciI GGCGGA 1 cut(s) 869
Eco130I CCWWGG 1 cut(s) 606
Eco31I GGTCTC 2 cut(s) 682, 1185
Eco47I GGWCC 2 cut(s) 519, 984
Eco57I CTGAAG 2 cut(s) 933, 1101
Eco88I CYCGRG 1 cut(s) 877
EcoO109I RGGNCCY 2 cut(s) 181, 218
EcoRII CCWGG 3 cut(s) 625, 1081, 1291
EcoT14I CCWWGG 1 cut(s) 606
EcoT22I ATGCAT 2 cut(s) 288, 1353
ErhI CCWWGG 1 cut(s) 606
FalI AAGNNNNNCTT 2 cut(s) 125, 157
FbaI TGATCA 2 cut(s) 753, 970
FblI GTMKAC 1 cut(s) 930
Fnu4HI GCNGC 3 cut(s) 696, 1287, 1404
FokI GGATG 6 cut(s) 344, 509, 588, 647, 698, 884
Fsp4HI GCNGC 3 cut(s) 696, 1287, 1404
FspBI CTAG 6 cut(s) 32, 480, 560, 681, 1274, 1356
GluI GCNGC 3 cut(s) 696, 1287, 1404
GsuI CTGGAG 1 cut(s) 1275
HaeIII GGCC 5 cut(s) 182, 220, 630, 805, 1368
HapII CCGG 3 cut(s) 376, 632, 987
HgaI GACGC 1 cut(s) 1172
HincII GTYRAC 2 cut(s) 931, 1426
HindII GTYRAC 2 cut(s) 931, 1426
HinfI GANTC 5 cut(s) 379, 434, 500, 961, 1099
HpaI GTTAAC 1 cut(s) 1426
HpaII CCGG 3 cut(s) 376, 632, 987
HphI GGTGA 3 cut(s) 615, 832, 1194
Hpy166II GTNNAC 5 cut(s) 166, 405, 931, 1093, 1426
Hpy188I TCNGA 9 cut(s) 226, 274, 753, 768, 796, 952, 1064, 1104, 1384
Hpy188III TCNNGA 7 cut(s) 121, 376, 431, 869, 968, 987, 1274
Hpy8I GTNNAC 5 cut(s) 166, 405, 931, 1093, 1426
HpyAV CCTTC 2 cut(s) 231, 1058
HpyCH4III ACNGT 2 cut(s) 78, 1458
HpyCH4IV ACGT 1 cut(s) 1335
HpyF10VI GCNNNNNNNGC 4 cut(s) 179, 283, 627, 1365
HpyF3I CTNAG 6 cut(s) 86, 93, 225, 273, 299, 1235
HpySE526I ACGT 1 cut(s) 1335
Kpn2I TCCGGA 2 cut(s) 375, 986
Ksp22I TGATCA 2 cut(s) 753, 970
KspAI GTTAAC 1 cut(s) 1426
Kzo9I GATC 9 cut(s) 229, 337, 427, 484, 753, 856, 920, 970, 1295
LmnI GCTCC 2 cut(s) 1041, 1294
Lsp1109I GCAGC 3 cut(s) 682, 1298, 1415
LweI GCATC 1 cut(s) 862
MaeI CTAG 6 cut(s) 32, 480, 560, 681, 1274, 1356
MaeII ACGT 1 cut(s) 1335
MaeIII GTNAC 2 cut(s) 535, 1515
MalI GATC 9 cut(s) 231, 339, 429, 486, 755, 858, 922, 972, 1297
MboI GATC 9 cut(s) 229, 337, 427, 484, 753, 856, 920, 970, 1295
MboII GAAGA 8 cut(s) 121, 257, 347, 554, 781, 956, 1343, 1467
MfeI CAATTG 1 cut(s) 1505
MflI RGATCY 1 cut(s) 1295
MlyI GAGTC 2 cut(s) 388, 443
MmeI TCCRAC 2 cut(s) 107, 756
Mph1103I ATGCAT 2 cut(s) 288, 1353
MroI TCCGGA 2 cut(s) 375, 986
MseI TTAA 9 cut(s) 207, 239, 369, 657, 741, 831, 1002, 1425, 1549
MspI CCGG 3 cut(s) 376, 632, 987
MspR9I CCNGG 4 cut(s) 627, 632, 1083, 1293
MunI CAATTG 1 cut(s) 1505
Mva1269I GAATGC 1 cut(s) 55
MvaI CCWGG 3 cut(s) 627, 1083, 1293
MwoI GCNNNNNNNGC 4 cut(s) 179, 283, 627, 1365
NciI CCSGG 1 cut(s) 632
NcoI CCATGG 1 cut(s) 606
NdeII GATC 9 cut(s) 229, 337, 427, 484, 753, 856, 920, 970, 1295
NheI GCTAGC 1 cut(s) 1355
NlaIV GGNNCC 3 cut(s) 219, 780, 1539
NsiI ATGCAT 2 cut(s) 288, 1353
NspI RCATGY 1 cut(s) 1355
PaeI GCATGC 1 cut(s) 1355
PaeR7I CTCGAG 1 cut(s) 877
PasI CCCWGGG 1 cut(s) 1082
PcsI WCGNNNNNNNCGW 2 cut(s) 1060, 1180
PctI GAATGC 1 cut(s) 55
PfeI GAWTC 3 cut(s) 500, 961, 1099
PfoI TCCNGGA 1 cut(s) 1291
PkrI GCNGC 3 cut(s) 697, 1288, 1405
PleI GAGTC 2 cut(s) 387, 442
PpsI GAGTC 2 cut(s) 387, 442
PshBI ATTAAT 1 cut(s) 207
Psp6I CCWGG 3 cut(s) 625, 1081, 1291
PspGI CCWGG 3 cut(s) 625, 1081, 1291
PspN4I GGNNCC 3 cut(s) 219, 780, 1539
PspPI GGNCC 5 cut(s) 181, 218, 519, 629, 984
PspXI VCTCGAGB 1 cut(s) 877
PsuI RGATCY 1 cut(s) 1295
RsaI GTAC 1 cut(s) 1400
RsaNI GTAC 1 cut(s) 1399
SalI GTCGAC 1 cut(s) 929
SaqAI TTAA 9 cut(s) 207, 239, 369, 657, 741, 831, 1002, 1425, 1549
SatI GCNGC 3 cut(s) 696, 1287, 1404
Sau3AI GATC 9 cut(s) 229, 337, 427, 484, 753, 856, 920, 970, 1295
Sau96I GGNCC 5 cut(s) 181, 218, 519, 629, 984
SchI GAGTC 2 cut(s) 388, 443
ScrFI CCNGG 4 cut(s) 627, 632, 1083, 1293
SfaNI GCATC 1 cut(s) 862
Sfr274I CTCGAG 1 cut(s) 877
SinI GGWCC 2 cut(s) 519, 984
SlaI CTCGAG 1 cut(s) 877
SmlI CTYRAG 3 cut(s) 119, 877, 1468
SmoI CTYRAG 3 cut(s) 119, 877, 1468
SpeI ACTAGT 1 cut(s) 31
SphI GCATGC 1 cut(s) 1355
SsiI CCGC 1 cut(s) 854
SspI AATATT 1 cut(s) 994
SspMI CTAG 6 cut(s) 32, 480, 560, 681, 1274, 1356
StyD4I CCNGG 4 cut(s) 625, 630, 1081, 1291
StyI CCWWGG 1 cut(s) 606
TaaI ACNGT 2 cut(s) 78, 1458
TaiI ACGT 1 cut(s) 1338
TaqI TCGA 4 cut(s) 186, 878, 923, 930
TfiI GAWTC 3 cut(s) 500, 961, 1099
Tru1I TTAA 9 cut(s) 207, 239, 369, 657, 741, 831, 1002, 1425, 1549
Tru9I TTAA 9 cut(s) 207, 239, 369, 657, 741, 831, 1002, 1425, 1549
TscAI CASTG 2 cut(s) 81, 580
TseI GCWGC 3 cut(s) 695, 1286, 1403
TspRI CASTG 2 cut(s) 81, 580
VpaK11BI GGWCC 2 cut(s) 519, 984
VspI ATTAAT 1 cut(s) 207
XapI RAATTY 3 cut(s) 550, 650, 1004
XbaI TCTAGA 1 cut(s) 1273
XceI RCATGY 1 cut(s) 1355
XcmI CCANNNNNNNNNTGG 1 cut(s) 529
XhoI CTCGAG 1 cut(s) 877
XmiI GTMKAC 1 cut(s) 930
XspI CTAG 6 cut(s) 32, 480, 560, 681, 1274, 1356
Zsp2I ATGCAT 2 cut(s) 288, 1353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.