MD13G1216600.v1.1
ERF Family

Transducin WD40 repeat-like superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0007851
Physical Location & Seq
Forward (+)
20641119 .. 20645339
4221 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1216600.v1.1.491

Sequence Viewer

Length: 2892 bp
ATGTCGGCCTCATCGGCTCCCCGCCTCAAAGACCGCGGCGGCGCTGGCGCCGTCGCAGGGTCCAAAGCCGCTGCCGCTCTCAAACCATCCAAGCCCCTCACGCCGGTACCCTTTTCCGACAAGAGGTACTCCAGCGTCGGAAAAGAGAACCCTCTTTCTGGGACCGCCGCTTTCCGGACCTCGGCCAAGAAGCCCACGATCCGGCCCGTTCCACGCGTTGACAAGGCGTCTGTGTCTGTGGCAACCAGAGACGGCGGTGGCGAAACGCGTGCCCGGTGGTCCATGCCGTCGGCGCCGAGAGGTAGGAGCTCTAGTCCTTCTGAGTTTACAAGGGTACTTTGTCATACGGGTAAGGAGCGAAGGGTCTCAGTGGGTCGGGCCAGACCGGGTTCGGGTCTGAGTTCAGTGGGGGAGAGCGATAGAGTGGTGGCGAGTGCAGGGAAGGCTCTGAGTAATGTTAGGGGATCAGCGAGTGGGAAGCAGAGAAAGGGGTTTAGGGATTTGGATGTGAAGAAGAGTGAAGTGGGGGCGAATGGGATTAAGGTTTTGAGGGATATCAAAGAAATTGGTGAAGTTGATGTGAATTTAGAGAAAAATAATGCAACTTCTGGAGAATTAGAAGTGAAAGGGGTGGGAATTGAGAAGAATTGGGATGGAGTTAGGGTTTCGGGAAGTGGTGGTGGAGTGTGTAAACTTACCTCCGAATTGAAAAACCCAGATGGGGTTGATAAAAAGGATCGAAATTTGGTGAGGGTTGATGACAAGGCTGTGAAATTTGGGAGTGGTGCTGTTTTGGGACTGAAGGAGTCTGGTGAAAAATCTGTGAGTAATGCAAAGGTTTTGGAGGGTTTGAAAGAAAAGAGGCTGAGTGAAGAAGGGCGTAGTGGCAGTCGTTCGGGTATCAAATATCCAAGTAAGCTTCATGAGAAGCTCGCTTTCTTGGAAGGGAAGGTTAAGAGGATTTCCTCGGATATTAAGAAGACAAAGGAGATTCTGGATATGAATAACACAGATACATCGAAGGTGATACTCTCCGATATTCAGGAAAAGATTTCCGGGATTGAGAAGGCCATGGGACATGCCAATGATTCAAGTGGTAAGATTGGGTTGCCGAAAAGTACTGAGCATAATGACCGAGATGCCAAAGTAGTTGAGAAGGAGCCGGTGAGTAATGCTAAGAGTTTTGTAGAAGGGTTGAACAGTGAAGACTTGGAAGCTAGGCTCTTTCCTCATCACAAGCTGCTTAAAAATCATAAGGCATTGAAAGGATCATCAGAAAGCTCTCAAAGTCATGGGTCCCAAGCTGTTGAAACCAGTTGTGAAGCAAACGTTGAAGAGAAGTCATTGAGCCTTATAGATGATAACCTAATTGCAGTAGAGTTCTTGGCTTCCTTGGACCGAACTAAAGTTTCAACAAGGGATGATGGATGTGAAGATTTGAAATGTTTTGAAGTTCAAGAAGTGGATGGTGTCACTGCTGCAGAAGTTGAAAAATCTTCAAAATTTGTCACTGGCAAGCTAAATTTGGAGCTCATTCTCACAACTGATGAAGCACTTGATGAGTTGGATGATCAGGAGAATGTACAGGAAATGATCATGGATGGGGAAACTGAGGATACCTGCATTTACCAGCTGAACCAAATAGGCCAAAAAACCTCAACTGGCGGGTGGTTTGTGTCTGAAGGAGAGTCGGTTCTTCTTGCTCATTATGACAGTTCATGCACCTTCTATGATATTGTAAACTGTGAGGAGAAGGTTGTGTATAAGCCTCCTGGTGGAGTTTCACCTAATATGTGGAGAGACTGTTGGATAATCCGTGCTCCCAGTGCTGATGGTTGCTCAGGAAAGTACGTTCTGGCTGCATCTGCTGGAAATACAATGGATTCAGGGTTTTGTTCATGGGATTTCTATGCCAAAGATGTGCGTGCTTTCCGGATTGAGGACTGTTCAGCCCCTTCAAGAACAGTGCTTGGCCCCTTACCTAACAACATTTCATATGGGAGAAATGCTTTGTCTGATCTTCTGGATCCAGAACCTAGACAATGGTGGTATAAACCTTGTGGACCTCTCATTGTCTCAACTGCTAGTTGTCAGAGAGTTGTGAGTATTTATGACATCCGTGATGGCGAACAAGTTATGAAATGGGATGTATCGAAGCCTGTTATAGCAATGGATAATTCAAGTCCCTTGCAGTGGAGAAATAGAGGAAAAGTTGTTGTAGCTGAAGCTGAAACAATTTCCCTTTGGGATGTGAACTCTCTTAACTCTCAGGCTTTACTATCTGTTTCTTCTTCTGGTCGGAAAATTTCTGCTCTTCATGTGAATAACACTGATGCTGAACTAGGTGGTGGCGTTCGACAAAGGGTAAGTTCTTCTGAGGCAGAAGGAAATGATGGAGTGTTTTGTACCCAAGATTCTATTAACCTTATAGACTTTCGGCATCCTACTGGTGTAGGTCTTAAGATACCAAAACTCGGTGTCAATGTGCAATCAGTCTTCTCCCGCGGAGATTCTGTCTTCCTTGGCTGCCCTAGTGCAAGATTGGGATGGAAAAAACAGTCCTCTTCACAGGTTCAGCAATTCTCAATCCGACAACAAAGGCTATACAGCACTTACGCTTTGCCAGAATCAAATGCTCACAGCCATTACACAGCAATTACGCAAGTTTGGGGGAATTCCAACCTCGTAATGGGTGTTTGTGGATTGGGGCTGTTTGTATTTGATGCATTGAAGGATGACGGAGTTCCATTGTTGACCAGTGATGATGGGACTCATAAAGCCAGAGAAACTGTTGGTCCAGATGACTTGTACGCACCTTCTTTCGATTACTTGGGTTCTCGGGCTCTTCTCATCTCAAGAGATCGCCCGGCACTGTGGAGGTACATACCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

964

Amino Acids

104.18

Weight (kDa)

7.75

Isoelectric Point (pI)

37.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_At4g14310 PF25465 677 - 958 7.6e-156 At4g14310 8-bladed propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1628
Acc65I GGTACC 1 cut(s) 106
AccB1I GGYRCC 3 cut(s) 47, 106, 292
AccBSI CCGCTC 1 cut(s) 77
AccII CGCG 4 cut(s) 36, 216, 268, 2505
AccIII TCCGGA 2 cut(s) 174, 1932
AclI AACGTT 1 cut(s) 1329
AclWI GGATC 6 cut(s) 193, 472, 744, 1276, 2021, 2034
AcoI YGGCCR 1 cut(s) 183
AcsI RAATTY 7 cut(s) 583, 742, 773, 1502, 1522, 2304, 2674
AcuI CTGAAG 3 cut(s) 821, 1701, 2244
AcyI GRCGYC 3 cut(s) 48, 227, 293
AfaI GTAC 9 cut(s) 108, 128, 336, 1120, 1584, 1850, 2407, 2810, 2882
AflII CTTAAG 1 cut(s) 2459
AflIII ACRYGT 2 cut(s) 214, 266
AhdI GACNNNNNGTC 1 cut(s) 226
AjnI CCWGG 1 cut(s) 1771
AloI GAACNNNNNNTCC 4 cut(s) 1677, 1709, 1836, 1868
Alw21I GWGCWC 3 cut(s) 311, 1533, 1822
Alw26I GTCTC 4 cut(s) 243, 370, 1794, 2080
AlwI GGATC 6 cut(s) 193, 472, 744, 1276, 2021, 2034
Ama87I CYCGRG 1 cut(s) 2838
Aor13HI TCCGGA 2 cut(s) 174, 1932
AoxI GGCC 7 cut(s) 6, 183, 203, 378, 1068, 1645, 1972
ApeKI GCWGC 5 cut(s) 71, 1240, 1478, 1859, 2526
ApoI RAATTY 7 cut(s) 583, 742, 773, 1502, 1522, 2304, 2674
Asp718I GGTACC 1 cut(s) 106
AspLEI GCGC 3 cut(s) 44, 50, 295
AsuC2I CCSGG 4 cut(s) 274, 387, 1057, 2867
AsuHPI GGTGA 6 cut(s) 581, 760, 824, 1036, 1177, 1776
AvaI CYCGRG 1 cut(s) 2838
AvaII GGWCC 8 cut(s) 60, 162, 177, 279, 1296, 1396, 2063, 2795
BaeGI GKGCMC 1 cut(s) 274
BaeI ACNNNNGTAYC 2 cut(s) 90, 123
BamHI GGATCC 1 cut(s) 2026
BanI GGYRCC 3 cut(s) 47, 106, 292
BanII GRGCYC 3 cut(s) 311, 1533, 2845
BbsI GAAGAC 4 cut(s) 986, 1212, 2488, 2509
Bbv12I GWGCWC 3 cut(s) 311, 1533, 1822
BbvI GCAGC 5 cut(s) 58, 1227, 1465, 1846, 2513
BceAI ACGGC 3 cut(s) 35, 268, 271
BcgI CGANNNNNNTGC 2 cut(s) 251, 285
BciT130I CCWGG 1 cut(s) 1773
BciVI GTATCC 1 cut(s) 1609
BclI TGATCA 2 cut(s) 1570, 1593
BcnI CCSGG 4 cut(s) 274, 387, 1057, 2867
BcoDI GTCTC 4 cut(s) 243, 370, 1794, 2080
BfaI CTAG 6 cut(s) 312, 1218, 2037, 2085, 2342, 2532
BfmI CTRYAG 1 cut(s) 1479
BfoI RGCGCY 3 cut(s) 45, 51, 296
BfrI CTTAAG 1 cut(s) 2459
BfuAI ACCTGC 1 cut(s) 1628
BfuI GTATCC 1 cut(s) 1609
BglI GCCNNNNNGGC 1 cut(s) 14
BmcAI AGTACT 1 cut(s) 1120
Bme1390I CCNGG 5 cut(s) 274, 387, 1057, 1773, 2867
Bme18I GGWCC 8 cut(s) 60, 162, 177, 279, 1296, 1396, 2063, 2795
BmeRI GACNNNNNGTC 1 cut(s) 226
BmeT110I CYCGRG 1 cut(s) 2838
BmrFI CCNGG 5 cut(s) 274, 387, 1057, 1773, 2867
BmrI ACTGGG 1 cut(s) 1818
BmsI GCATC 5 cut(s) 1129, 1871, 2323, 2449, 2713
BmuI ACTGGG 1 cut(s) 1818
BpiI GAAGAC 4 cut(s) 986, 1212, 2488, 2509
BpmI CTGGAG 2 cut(s) 115, 630
Bpu10I CCTNAGC 1 cut(s) 1840
BpuEI CTTGAG 1 cut(s) 2839
BpuMI CCSGG 4 cut(s) 274, 387, 1057, 2867
BsaHI GRCGYC 3 cut(s) 48, 227, 293
BsaI GGTCTC 1 cut(s) 370
BsaJI CCNNGG 7 cut(s) 34, 180, 966, 1071, 1392, 2503, 2521
BsaWI WCCGGW 2 cut(s) 174, 1932
Bse118I RCCGGY 2 cut(s) 103, 1162
Bse1I ACTGG 6 cut(s) 1314, 1516, 1666, 1824, 2452, 2757
Bse3DI GCAATG 1 cut(s) 2175
BseAI TCCGGA 2 cut(s) 174, 1932
BseBI CCWGG 1 cut(s) 1773
BseDI CCNNGG 7 cut(s) 34, 180, 966, 1071, 1392, 2503, 2521
BseMI GCAATG 1 cut(s) 2175
BseNI ACTGG 6 cut(s) 1314, 1516, 1666, 1824, 2452, 2757
BseRI GAGGAG 1 cut(s) 1763
BseSI GKGCMC 1 cut(s) 274
BseXI GCAGC 5 cut(s) 58, 1227, 1465, 1846, 2513
BsgI GTGCAG 1 cut(s) 456
Bsh1236I CGCG 4 cut(s) 36, 216, 268, 2505
BshFI GGCC 7 cut(s) 8, 185, 205, 380, 1070, 1647, 1974
BshNI GGYRCC 3 cut(s) 47, 106, 292
BsiHKAI GWGCWC 3 cut(s) 311, 1533, 1822
BsiHKCI CYCGRG 1 cut(s) 2838
BsiSI CCGG 9 cut(s) 104, 175, 202, 274, 386, 1056, 1163, 1933, 2867
BslFI GGGAC 6 cut(s) 175, 810, 1089, 1282, 2169, 2782
BsmAI GTCTC 4 cut(s) 243, 370, 1794, 2080
BsmBI CGTCTC 1 cut(s) 243
BsmFI GGGAC 6 cut(s) 175, 810, 1089, 1282, 2169, 2782
BsnI GGCC 7 cut(s) 8, 185, 205, 380, 1070, 1647, 1974
Bso31I GGTCTC 1 cut(s) 370
BsoBI CYCGRG 1 cut(s) 2838
Bsp1286I GDGCHC 5 cut(s) 274, 311, 1533, 1822, 2845
Bsp13I TCCGGA 2 cut(s) 174, 1932
Bsp1407I TGTACA 1 cut(s) 1582
Bsp143I GATC 9 cut(s) 198, 464, 736, 1268, 1570, 1593, 2017, 2026, 2860
Bsp19I CCATGG 1 cut(s) 1071
BspANI GGCC 7 cut(s) 8, 185, 205, 380, 1070, 1647, 1974
BspEI TCCGGA 2 cut(s) 174, 1932
BspFNI CGCG 4 cut(s) 36, 216, 268, 2505
BspHI TCATGA 1 cut(s) 922
BspMAI CTGCAG 1 cut(s) 1483
BspMI ACCTGC 1 cut(s) 1628
BspPI GGATC 6 cut(s) 193, 472, 744, 1276, 2021, 2034
BspQI GCTCTTC 2 cut(s) 2319, 2850
BspT107I GGYRCC 3 cut(s) 47, 106, 292
BspTI CTTAAG 1 cut(s) 2459
BspTNI GGTCTC 1 cut(s) 370
BsrBI CCGCTC 1 cut(s) 77
BsrDI GCAATG 1 cut(s) 2175
BsrFI RCCGGY 2 cut(s) 103, 1162
BsrGI TGTACA 1 cut(s) 1582
BsrI ACTGG 6 cut(s) 1314, 1516, 1666, 1824, 2452, 2757
BssAI RCCGGY 2 cut(s) 103, 1162
BssECI CCNNGG 7 cut(s) 34, 180, 966, 1071, 1392, 2503, 2521
BssMI GATC 9 cut(s) 198, 464, 736, 1268, 1570, 1593, 2017, 2026, 2860
BssNI GRCGYC 3 cut(s) 48, 227, 293
BssT1I CCWWGG 3 cut(s) 1071, 1392, 2521
Bst2UI CCWGG 1 cut(s) 1773
Bst4CI ACNGT 9 cut(s) 1202, 1715, 1745, 1805, 1946, 1966, 2559, 2791, 2874
Bst6I CTCTTC 5 cut(s) 509, 1329, 2319, 2569, 2850
BstACI GRCGYC 3 cut(s) 48, 227, 293
BstAFI CTTAAG 1 cut(s) 2459
BstAUI TGTACA 1 cut(s) 1582
BstC8I GCNNGC 5 cut(s) 46, 270, 933, 1517, 1926
BstDSI CCRYGG 3 cut(s) 34, 1071, 2503
BstFNI CGCG 4 cut(s) 36, 216, 268, 2505
BstH2I RGCGCY 3 cut(s) 45, 51, 296
BstHHI GCGC 3 cut(s) 44, 50, 295
BstKTI GATC 9 cut(s) 201, 467, 739, 1271, 1573, 1596, 2020, 2029, 2863
BstMAI GTCTC 4 cut(s) 243, 370, 1794, 2080
BstMBI GATC 9 cut(s) 198, 464, 736, 1268, 1570, 1593, 2017, 2026, 2860
BstMWI GCNNNNNNNGC 8 cut(s) 14, 45, 74, 100, 292, 443, 1826, 1865
BstNI CCWGG 1 cut(s) 1773
BstNSI RCATGY 1 cut(s) 1082
BstSCI CCNGG 5 cut(s) 272, 385, 1055, 1771, 2865
BstSFI CTRYAG 1 cut(s) 1479
BstSLI GKGCMC 1 cut(s) 274
BstUI CGCG 4 cut(s) 36, 216, 268, 2505
BstV1I GCAGC 5 cut(s) 58, 1227, 1465, 1846, 2513
BstV2I GAAGAC 4 cut(s) 986, 1212, 2488, 2509
BstX2I RGATCY 1 cut(s) 2026
BstYI RGATCY 1 cut(s) 2026
BsuI GTATCC 1 cut(s) 1609
BsuRI GGCC 7 cut(s) 8, 185, 205, 380, 1070, 1647, 1974
BtgI CCRYGG 3 cut(s) 34, 1071, 2503
BtsI GCAGTG 2 cut(s) 1473, 2198
BveI ACCTGC 1 cut(s) 1628
Cac8I GCNNGC 5 cut(s) 46, 270, 933, 1517, 1926
CciI TCATGA 1 cut(s) 922
CfoI GCGC 3 cut(s) 44, 50, 295
Cfr10I RCCGGY 2 cut(s) 103, 1162
Cfr42I CCGCGG 2 cut(s) 37, 2506
CseI GACGC 2 cut(s) 124, 216
Csp6I GTAC 9 cut(s) 107, 127, 335, 1119, 1583, 1849, 2406, 2809, 2881
CviAII CATG 9 cut(s) 283, 923, 1072, 1079, 1292, 1597, 1719, 1899, 2318
CviQI GTAC 9 cut(s) 107, 127, 335, 1119, 1583, 1849, 2406, 2809, 2881
DinI GGCGCC 2 cut(s) 49, 294
DpnI GATC 9 cut(s) 200, 466, 738, 1270, 1572, 1595, 2019, 2028, 2862
DpnII GATC 9 cut(s) 198, 464, 736, 1268, 1570, 1593, 2017, 2026, 2860
DriI GACNNNNNGTC 1 cut(s) 226
EaeI YGGCCR 1 cut(s) 183
Eam1104I CTCTTC 5 cut(s) 509, 1329, 2319, 2569, 2850
Eam1105I GACNNNNNGTC 1 cut(s) 226
EarI CTCTTC 5 cut(s) 509, 1329, 2319, 2569, 2850
Ecl136II GAGCTC 2 cut(s) 309, 1531
Eco130I CCWWGG 3 cut(s) 1071, 1392, 2521
Eco24I GRGCYC 3 cut(s) 311, 1533, 2845
Eco31I GGTCTC 1 cut(s) 370
Eco32I GATATC 1 cut(s) 556
Eco47I GGWCC 8 cut(s) 60, 162, 177, 279, 1296, 1396, 2063, 2795
Eco53kI GAGCTC 2 cut(s) 309, 1531
Eco57I CTGAAG 3 cut(s) 821, 1701, 2244
Eco88I CYCGRG 1 cut(s) 2838
EcoICRI GAGCTC 2 cut(s) 309, 1531
EcoO109I RGGNCCY 1 cut(s) 1296
EcoRI GAATTC 1 cut(s) 2674
EcoRII CCWGG 1 cut(s) 1771
EcoRV GATATC 1 cut(s) 556
EcoT14I CCWWGG 3 cut(s) 1071, 1392, 2521
EcoT22I ATGCAT 1 cut(s) 2728
EcoT38I GRGCYC 3 cut(s) 311, 1533, 2845
EgeI GGCGCC 2 cut(s) 49, 294
EheI GGCGCC 2 cut(s) 49, 294
ErhI CCWWGG 3 cut(s) 1071, 1392, 2521
Esp3I CGTCTC 1 cut(s) 243
FaeI CATG 9 cut(s) 286, 926, 1075, 1082, 1295, 1600, 1722, 1902, 2321
FaqI GGGAC 6 cut(s) 175, 810, 1089, 1282, 2169, 2782
FatI CATG 9 cut(s) 282, 922, 1071, 1078, 1291, 1596, 1718, 1898, 2317
FauI CCCGC 3 cut(s) 29, 1658, 2510
FauNDI CATATG 1 cut(s) 1996
FbaI TGATCA 2 cut(s) 1570, 1593
FriOI GRGCYC 3 cut(s) 311, 1533, 2845
FspBI CTAG 6 cut(s) 312, 1218, 2037, 2085, 2342, 2532
GlaI GCGC 3 cut(s) 43, 49, 294
GsuI CTGGAG 2 cut(s) 115, 630
HaeII RGCGCY 3 cut(s) 45, 51, 296
HaeIII GGCC 7 cut(s) 8, 185, 205, 380, 1070, 1647, 1974
HapII CCGG 9 cut(s) 104, 175, 202, 274, 386, 1056, 1163, 1933, 2867
HgaI GACGC 2 cut(s) 124, 216
HhaI GCGC 3 cut(s) 44, 50, 295
Hin1I GRCGYC 3 cut(s) 48, 227, 293
Hin1II CATG 9 cut(s) 286, 926, 1075, 1082, 1295, 1600, 1722, 1902, 2321
Hin6I GCGC 3 cut(s) 42, 48, 293
HinP1I GCGC 3 cut(s) 42, 48, 293
HincII GTYRAC 2 cut(s) 220, 2754
HindII GTYRAC 2 cut(s) 220, 2754
HindIII AAGCTT 1 cut(s) 917
HinfI GANTC 9 cut(s) 806, 991, 1088, 1688, 1883, 2414, 2510, 2627, 2770
HpaII CCGG 9 cut(s) 104, 175, 202, 274, 386, 1056, 1163, 1933, 2867
HphI GGTGA 6 cut(s) 581, 760, 824, 1036, 1177, 1776
Hpy166II GTNNAC 7 cut(s) 220, 327, 692, 1741, 2063, 2254, 2754
Hpy8I GTNNAC 7 cut(s) 220, 327, 692, 1741, 2063, 2254, 2754
Hpy99I CGWCG 3 cut(s) 56, 140, 292
HpyCH4III ACNGT 9 cut(s) 1202, 1715, 1745, 1805, 1946, 1966, 2559, 2791, 2874
HpyCH4IV ACGT 2 cut(s) 1329, 1851
HpyF10VI GCNNNNNNNGC 8 cut(s) 14, 45, 74, 100, 292, 443, 1826, 1865
HpySE526I ACGT 2 cut(s) 1329, 1851
Hsp92I GRCGYC 3 cut(s) 48, 227, 293
Hsp92II CATG 9 cut(s) 286, 926, 1075, 1082, 1295, 1600, 1722, 1902, 2321
HspAI GCGC 3 cut(s) 42, 48, 293
KasI GGCGCC 2 cut(s) 47, 292
KflI GGGWCCC 1 cut(s) 1296
Kpn2I TCCGGA 2 cut(s) 174, 1932
KpnI GGTACC 1 cut(s) 110
Ksp22I TGATCA 2 cut(s) 1570, 1593
KspI CCGCGG 2 cut(s) 37, 2506
Kzo9I GATC 9 cut(s) 198, 464, 736, 1268, 1570, 1593, 2017, 2026, 2860
LguI GCTCTTC 2 cut(s) 2319, 2850
LmnI GCTCC 6 cut(s) 22, 306, 355, 1159, 1528, 1825
Lsp1109I GCAGC 5 cut(s) 58, 1227, 1465, 1846, 2513
LweI GCATC 5 cut(s) 1129, 1871, 2323, 2449, 2713
MaeI CTAG 6 cut(s) 312, 1218, 2037, 2085, 2342, 2532
MaeII ACGT 2 cut(s) 1329, 1851
MaeIII GTNAC 2 cut(s) 1471, 1507
MalI GATC 9 cut(s) 200, 466, 738, 1270, 1572, 1595, 2019, 2028, 2862
MbiI CCGCTC 1 cut(s) 77
MboI GATC 9 cut(s) 198, 464, 736, 1268, 1570, 1593, 2017, 2026, 2860
MflI RGATCY 1 cut(s) 2026
MhlI GDGCHC 5 cut(s) 274, 311, 1533, 1822, 2845
MluI ACGCGT 2 cut(s) 214, 266
Mly113I GGCGCC 2 cut(s) 48, 293
MlyI GAGTC 3 cut(s) 815, 1697, 2764
MmeI TCCRAC 7 cut(s) 118, 141, 1545, 1787, 2279, 2614, 2703
Mph1103I ATGCAT 1 cut(s) 2728
MroI TCCGGA 2 cut(s) 174, 1932
MseI TTAA 7 cut(s) 540, 954, 975, 1245, 2262, 2421, 2460
MslI CAYNNNNRTG 1 cut(s) 1083
MspA1I CMGCKG 4 cut(s) 36, 71, 1633, 2505
MspCI CTTAAG 1 cut(s) 2459
MspI CCGG 9 cut(s) 104, 175, 202, 274, 386, 1056, 1163, 1933, 2867
MspR9I CCNGG 5 cut(s) 274, 387, 1057, 1773, 2867
MvaI CCWGG 1 cut(s) 1773
MvnI CGCG 4 cut(s) 36, 216, 268, 2505
MwoI GCNNNNNNNGC 8 cut(s) 14, 45, 74, 100, 292, 443, 1826, 1865
NarI GGCGCC 2 cut(s) 48, 293
NciI CCSGG 4 cut(s) 274, 387, 1057, 2867
NcoI CCATGG 1 cut(s) 1071
NdeI CATATG 1 cut(s) 1996
NdeII GATC 9 cut(s) 198, 464, 736, 1268, 1570, 1593, 2017, 2026, 2860
NlaIII CATG 9 cut(s) 286, 926, 1075, 1082, 1295, 1600, 1722, 1902, 2321
NmeAIII GCCGAG 2 cut(s) 161, 321
NmuCI GTSAC 2 cut(s) 1471, 1507
NsiI ATGCAT 1 cut(s) 2728
NspI RCATGY 1 cut(s) 1082
PagI TCATGA 1 cut(s) 922
PciSI GCTCTTC 2 cut(s) 2319, 2850
PcsI WCGNNNNNNNCGW 1 cut(s) 258
PfeI GAWTC 6 cut(s) 991, 1088, 1883, 2414, 2510, 2627
PfoI TCCNGGA 1 cut(s) 1055
PleI GAGTC 3 cut(s) 814, 1696, 2764
PluTI GGCGCC 2 cut(s) 51, 296
PpsI GAGTC 3 cut(s) 814, 1696, 2764
PpuMI RGGWCCY 1 cut(s) 1296
Psp124BI GAGCTC 2 cut(s) 311, 1533
Psp1406I AACGTT 1 cut(s) 1329
Psp5II RGGWCCY 1 cut(s) 1296
Psp6I CCWGG 1 cut(s) 1771
PspGI CCWGG 1 cut(s) 1771
PspPPI RGGWCCY 1 cut(s) 1296
PstI CTGCAG 1 cut(s) 1483
PsuI RGATCY 1 cut(s) 2026
PvuII CAGCTG 1 cut(s) 1633
RsaI GTAC 9 cut(s) 108, 128, 336, 1120, 1584, 1850, 2407, 2810, 2882
RsaNI GTAC 9 cut(s) 107, 127, 335, 1119, 1583, 1849, 2406, 2809, 2881
RseI CAYNNNNRTG 1 cut(s) 1083
SacI GAGCTC 2 cut(s) 311, 1533
SacII CCGCGG 2 cut(s) 37, 2506
SapI GCTCTTC 2 cut(s) 2319, 2850
SaqAI TTAA 7 cut(s) 540, 954, 975, 1245, 2262, 2421, 2460
Sau3AI GATC 9 cut(s) 198, 464, 736, 1268, 1570, 1593, 2017, 2026, 2860
ScaI AGTACT 1 cut(s) 1120
SchI GAGTC 3 cut(s) 815, 1697, 2764
ScrFI CCNGG 5 cut(s) 274, 387, 1057, 1773, 2867
SduI GDGCHC 5 cut(s) 274, 311, 1533, 1822, 2845
SfaNI GCATC 5 cut(s) 1129, 1871, 2323, 2449, 2713
SfcI CTRYAG 1 cut(s) 1479
SfoI GGCGCC 2 cut(s) 49, 294
Sfr303I CCGCGG 2 cut(s) 37, 2506
SgrBI CCGCGG 2 cut(s) 37, 2506
SinI GGWCC 8 cut(s) 60, 162, 177, 279, 1296, 1396, 2063, 2795
SmiMI CAYNNNNRTG 1 cut(s) 1083
SmlI CTYRAG 2 cut(s) 2459, 2854
SmoI CTYRAG 2 cut(s) 2459, 2854
SspDI GGCGCC 2 cut(s) 47, 292
SspMI CTAG 6 cut(s) 312, 1218, 2037, 2085, 2342, 2532
SstI GAGCTC 2 cut(s) 311, 1533
StyD4I CCNGG 5 cut(s) 272, 385, 1055, 1771, 2865
StyI CCWWGG 3 cut(s) 1071, 1392, 2521
TaaI ACNGT 9 cut(s) 1202, 1715, 1745, 1805, 1946, 1966, 2559, 2791, 2874
TaiI ACGT 2 cut(s) 1332, 1854
TaqI TCGA 5 cut(s) 739, 1019, 2153, 2356, 2823
TaqII GACCGA 2 cut(s) 1149, 1413
TatI WGTACW 2 cut(s) 1118, 1582
TauI GCSGC 5 cut(s) 39, 42, 71, 77, 170
TfiI GAWTC 6 cut(s) 991, 1088, 1883, 2414, 2510, 2627
Tru1I TTAA 7 cut(s) 540, 954, 975, 1245, 2262, 2421, 2460
Tru9I TTAA 7 cut(s) 540, 954, 975, 1245, 2262, 2421, 2460
TseFI GTSAC 2 cut(s) 1471, 1507
TseI GCWGC 5 cut(s) 71, 1240, 1478, 1859, 2526
Tsp45I GTSAC 2 cut(s) 1471, 1507
TspDTI ATGAA 8 cut(s) 911, 1016, 1563, 1707, 1887, 1983, 2153, 2306
TspGWI ACGGA 3 cut(s) 1805, 2108, 2754
Vha464I CTTAAG 1 cut(s) 2459
VpaK11BI GGWCC 8 cut(s) 60, 162, 177, 279, 1296, 1396, 2063, 2795
XapI RAATTY 7 cut(s) 583, 742, 773, 1502, 1522, 2304, 2674
XceI RCATGY 1 cut(s) 1082
XcmI CCANNNNNNNNNTGG 1 cut(s) 2686
XspI CTAG 6 cut(s) 312, 1218, 2037, 2085, 2342, 2532
ZrmI AGTACT 1 cut(s) 1120
Zsp2I ATGCAT 1 cut(s) 2728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.