MD16G1222000.v1.1
ERF Family

Transducin WD40 repeat-like superfamily protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0007851
Physical Location & Seq
Forward (+)
22185645 .. 22189732
4088 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1222000.v1.1.491

Sequence Viewer

Length: 2892 bp
ATGTCGGCCACATCTGCTCGCCGCTTCAAAGACCGCGGCGGCGCCGGCGTGGCAGCATCCAAACCCGCCGCCACTCTCAAACCATCCAAGCCTCTAACGCCGATACCCTTTTCCGACAAGAGGTACGTCAGCGCCGGAAAGGAGAACCCTCTTCCTGGGACCGCTGCTTTCCGGGCTTCGGCCCAGAAGCCCACAATCCGGCCCGTTTCTCGCGTCGACAAGGCGGCTGTGTCTGCGGCAACCAGTGGCGCAGGTAGCGAAACGCGTTCGCGGTGGTCCATGTCATCGGCGCCGAGAGGTAGGAGCTCTAGCCCTTCTGAGTTTACTAGGGTAATTTCTCATACGGGTAAGGAGCGGAGGGTCTCGGTGGATCGGGTCAGACCGGGTTCGGGTCTGATTTCGGTTGGGGAGAGGGATCGCGTAGTGTCGAGTGCAGGGAAGGTTAGAGGATCAGCTAGTGGGAAGCAGAGAACAGGGTTTAGGGATTTGGATGTGAAGAGGAGTGATGTGGGAGCGAATGGGATTAGGGTTTTGAGGGATATCAAAGAAAGTGGTAAAATTGGTGTGAATTTAGAGAAAAAGAATGCAACTTCTGGAGAATTAGAAGTGAGAGCGGTGGAAATTGAGAAGAATTCGGATGGGGTTAGGGTTAGGGATCCGGGAAGTGGAGATGGAGAGGCTAATTTGAGCTCCGATCTGAAAAACCCTAGTGCGGTTGATAAAAGGGATCAAAATTTGGCGAGGGTGGATGATAAAGCTGTGAAAATTGGGAGTGGTGATGCTTTGGGACTGAAGGAGTCTGGTGAAAAATCTGTAAGTGATGCAAAGGTTTTGGAGGGTTTGAAAGAAAAGAGGTTGAATGAAGAAGGTAGTAGTGGCGGTCGTTCGGGTATCAAATATCCGAGTAAGCTTCACGAGAAGCTTGCTTTCTTGGAAGGGAAGGTTAAGAGGATTGCCTCGGATATTAAGAAGACCAAGGAGATTTTGGATATGAATAATCCAGATACGTCGAAGGTGATACTCTCTGATATTCAGGAAAAGATTTCTGGAATTGAGAAGGCCATGAGACATGTCAATGATTCAGGTGGTAAGATGGGGTTGCCGAAAAGTACTGAGCATAGTGACAGGGATGCAAAAGTAGTTGAGAAGGGCCATATTGAGCTGGTGTGTAATGCTAAGAGTTTGGTAAAAGGGTTGAACAGTGAGGACTTGGAAGCTAGGCTCTTTCCTCATCATAAGTTGCTTAAAAACTGTGTGGCGTTGAAAGAATCATTCGAAAGCTCTCAAAGTCATGGGTTCCAAGCTGTTGAAACTAGTTGTGAAGCAAAGGTTGAAGAGAAGTCATTGAGCCTTACAGATGATAACCCAATTGCGGTAGAGTTCTTGGCTTCCTTGGACCAAACTAAAGTTTCAACAAGGGATGACGGACGTGAAGGTTTGGAATGTTTTGAGGTTCAAGAAGTGGATGGTGTCACTGCTGCAGAAGTTGAAAAATCTTCAAAATTTGTCTCTGGAAAACAAAATTTGGAGCTCATTCTCACAACCGATGAAAAACTTGATGAGTTTGATGATCAGGAGAATAGACAGGAAATGTTCTTTGACGAGGAAAATGAGGATACTTGCAGTTACCAGCTGAACCAAATAGGCCAAAAAACCTCAACTGGAGGGTGGTTCATGTCTGAGGGAGAGTCGGTTCTTCTTGCTCACGATGATAGTTCGTGCACCTTCTATGATATTGTAAACTGTGAGGAGAAAGTTGTGTACAAGCCTCCTGCTGTAGTTTCACCTAATATGTGGAGAGACTGTTGGATTATCCGTGCTCCCAGTGCTGATGGTTGCTCAGGAAGGTATGTTGTGGCTGCATCAGCTGGCAATACAATGGATGCAGGATTTTGTTCATGGGATTTCTATGCCGAAGATGTGCGTGCTTTCCGGATTGAGGATGGTTCAGCCCCTTCAAGAACAGTGCTTGGCCGCTTGCCCAACAGCATCTCACATGGAAGAAATGCTTTGTCTGATCTTCTGGATCCAGAACCTAGACAATGGTGGTATAGACCTTGTGGACCTCTCATTGTCTCAACTGCTAGCTGTCAGAGAGTTGTGAGAATCTATGACATCCGTGATGGTGAGCAAGTTATGAAATGGGATGTATCAAAGCCTGTTATAGCAATGGATAATTCAAGCCCCTTGCAGTGGAGAAATAGAGGAAAAGTTGTTGTAGCTGAAGCTGAAACAATTTCCCTTTGGGATGTGAACTCTCTTAACCCTCAAGCTTTACTATCTGTTTCTTCTTCTGGTCGGAAAATTTCCGCTCTTCATGTGAATAACACTGATGCTGAGCTAGGTGGTGGTGTTCGACAAAGAGTAAGTTCTGTGGAAGCAGAAGGAAATGATGGAGTGTTCTGTACTCAAGATTCTATTAACATTATAGACTTTCGGCATCCAACTGGCGTTGGTCTTAAGATACCAAAACTCGGTGTAAATGTGCAATCAGTTTTCTCTCGTGGAGATTCTGTCTTCCTTGGCTGCACTAGTGCAAGATGGGGATGGAAAAAGCAGTCTTCTTCACAGGTGCAACAATTTTCAATCCGAAAACAAAGTCTATACAGCACCTACGCTTTGCCAGAATCAAATGCTCACAGTCATTACACAGCAATTACACAAGTTTGGGGGAATTCAAACCTTATCATGGGTGTTTGCGGATTGGGGCTGTTTGTATTTGATGCGTTGAAGGATGACGGAGTTCCATTGTTGACCAGTGATGACGGGAGTCAAAAAGCCAGAGAAACTGTTGGTCCAGATGACTTGTATGCACCTTCTTTTGATTACTTGGATTCTCGGGCACTTCTTATCTCAAGGGATCGCCCGGCACTGTGGAGGAACCTATTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

964

Amino Acids

104.92

Weight (kDa)

6.75

Isoelectric Point (pI)

40.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_At4g14310 PF25465 677 - 958 4.9e-156 At4g14310 8-bladed propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 242
AccB1I GGYRCC 2 cut(s) 41, 289
AccBSI CCGCTC 3 cut(s) 355, 614, 2312
AccI GTMKAC 1 cut(s) 216
AccII CGCG 5 cut(s) 36, 213, 265, 271, 420
AccIII TCCGGA 1 cut(s) 1932
AclWI GGATC 9 cut(s) 378, 423, 457, 650, 663, 735, 2021, 2034, 2868
AcoI YGGCCR 2 cut(s) 6, 1972
AcsI RAATTY 7 cut(s) 568, 631, 733, 1502, 1522, 2304, 2674
AcuI CTGAAG 2 cut(s) 812, 2244
AcyI GRCGYC 2 cut(s) 42, 290
AfaI GTAC 4 cut(s) 125, 1111, 1763, 2407
AflII CTTAAG 1 cut(s) 2459
AflIII ACRYGT 2 cut(s) 263, 1069
AhlI ACTAGT 2 cut(s) 1313, 2531
AjiI CACGTC 1 cut(s) 1430
AjnI CCWGG 1 cut(s) 154
AloI GAACNNNNNNTCC 2 cut(s) 1677, 1709
Alw21I GWGCWC 5 cut(s) 308, 692, 1533, 1724, 1822
Alw26I GTCTC 5 cut(s) 367, 1060, 1513, 1794, 2080
Alw44I GTGCAC 1 cut(s) 1720
AlwI GGATC 9 cut(s) 378, 423, 457, 650, 663, 735, 2021, 2034, 2868
Ama87I CYCGRG 1 cut(s) 2838
Aor13HI TCCGGA 1 cut(s) 1932
AoxI GGCC 7 cut(s) 6, 180, 200, 1059, 1150, 1645, 1972
ApaLI GTGCAC 1 cut(s) 1720
ApeKI GCWGC 5 cut(s) 53, 164, 1478, 1859, 2526
ApoI RAATTY 7 cut(s) 568, 631, 733, 1502, 1522, 2304, 2674
ArsI GACNNNNNNTTYG 2 cut(s) 2584, 2616
AspLEI GCGC 4 cut(s) 44, 134, 251, 292
AspS9I GGNCC 8 cut(s) 159, 181, 201, 276, 1150, 1396, 2063, 2795
AsuC2I CCSGG 4 cut(s) 173, 384, 660, 2867
AsuHPI GGTGA 5 cut(s) 788, 815, 1027, 1776, 2138
AsuII TTCGAA 1 cut(s) 1275
AsuNHI GCTAGC 1 cut(s) 2084
AvaI CYCGRG 1 cut(s) 2838
AvaII GGWCC 5 cut(s) 159, 276, 1396, 2063, 2795
BaeGI GKGCMC 2 cut(s) 1724, 2845
BamHI GGATCC 2 cut(s) 655, 2026
BanI GGYRCC 2 cut(s) 41, 289
BanII GRGCYC 3 cut(s) 308, 692, 1533
BauI CACGAG 2 cut(s) 914, 2502
BbsI GAAGAC 3 cut(s) 977, 2509, 2553
Bbv12I GWGCWC 5 cut(s) 308, 692, 1533, 1724, 1822
BbvI GCAGC 5 cut(s) 65, 151, 1465, 1846, 2513
BcgI CGANNNNNNTGC 2 cut(s) 905, 939
BciT130I CCWGG 1 cut(s) 156
BciVI GTATCC 1 cut(s) 1609
BclI TGATCA 1 cut(s) 1570
BcnI CCSGG 4 cut(s) 173, 384, 660, 2867
BcoDI GTCTC 5 cut(s) 367, 1060, 1513, 1794, 2080
BcuI ACTAGT 2 cut(s) 1313, 2531
BfmI CTRYAG 2 cut(s) 1479, 1776
BfoI RGCGCY 3 cut(s) 45, 135, 293
BfrI CTTAAG 1 cut(s) 2459
BfuAI ACCTGC 1 cut(s) 242
BfuI GTATCC 1 cut(s) 1609
BglI GCCNNNNNGGC 1 cut(s) 50
BlpI GCTNAGC 1 cut(s) 2337
BmcAI AGTACT 1 cut(s) 1111
Bme1390I CCNGG 5 cut(s) 156, 173, 384, 660, 2867
Bme18I GGWCC 5 cut(s) 159, 276, 1396, 2063, 2795
BmeT110I CYCGRG 1 cut(s) 2838
BmgBI CACGTC 1 cut(s) 1430
BmgT120I GGNCC 8 cut(s) 159, 181, 201, 276, 1150, 1396, 2063, 2795
BmiI GGNNCC 7 cut(s) 43, 160, 291, 657, 1298, 2028, 2882
BmrFI CCNGG 5 cut(s) 156, 173, 384, 660, 2867
BmrI ACTGGG 1 cut(s) 1818
BmtI GCTAGC 1 cut(s) 2088
BmuI ACTGGG 1 cut(s) 1818
BoxI GACNNNNGTC 1 cut(s) 2769
BpiI GAAGAC 3 cut(s) 977, 2509, 2553
BpmI CTGGAG 2 cut(s) 615, 1683
Bpu10I CCTNAGC 1 cut(s) 1840
Bpu1102I GCTNAGC 1 cut(s) 2337
Bpu14I TTCGAA 1 cut(s) 1275
BpuEI CTTGAG 3 cut(s) 2253, 2394, 2839
BpuMI CCSGG 4 cut(s) 173, 384, 660, 2867
BsaHI GRCGYC 2 cut(s) 42, 290
BsaI GGTCTC 1 cut(s) 367
BsaJI CCNNGG 6 cut(s) 34, 155, 957, 975, 1392, 2521
BsaWI WCCGGW 1 cut(s) 1932
BsaXI ACNNNNNCTCC 6 cut(s) 1677, 1707, 1789, 1819, 2499, 2529
Bse118I RCCGGY 1 cut(s) 44
Bse1I ACTGG 5 cut(s) 243, 1666, 1824, 2452, 2757
Bse3DI GCAATG 1 cut(s) 2175
BseAI TCCGGA 1 cut(s) 1932
BseBI CCWGG 1 cut(s) 156
BseDI CCNNGG 6 cut(s) 34, 155, 957, 975, 1392, 2521
BseMI GCAATG 1 cut(s) 2175
BseMII CTCAG 5 cut(s) 309, 1104, 1671, 1854, 2328
BseNI ACTGG 5 cut(s) 243, 1666, 1824, 2452, 2757
BseRI GAGGAG 2 cut(s) 514, 1763
BseSI GKGCMC 2 cut(s) 1724, 2845
BseXI GCAGC 5 cut(s) 65, 151, 1465, 1846, 2513
BsgI GTGCAG 2 cut(s) 453, 2512
Bsh1236I CGCG 5 cut(s) 36, 213, 265, 271, 420
Bsh1285I CGRYCG 1 cut(s) 883
BshFI GGCC 7 cut(s) 8, 182, 202, 1061, 1152, 1647, 1974
BshNI GGYRCC 2 cut(s) 41, 289
BsiEI CGRYCG 1 cut(s) 883
BsiHKAI GWGCWC 5 cut(s) 308, 692, 1533, 1724, 1822
BsiHKCI CYCGRG 1 cut(s) 2838
BsiSI CCGG 8 cut(s) 45, 135, 172, 199, 383, 659, 1933, 2867
BslFI GGGAC 2 cut(s) 172, 801
BsmAI GTCTC 5 cut(s) 367, 1060, 1513, 1794, 2080
BsmFI GGGAC 2 cut(s) 172, 801
BsmI GAATGC 1 cut(s) 589
BsnI GGCC 7 cut(s) 8, 182, 202, 1061, 1152, 1647, 1974
Bso31I GGTCTC 1 cut(s) 367
BsoBI CYCGRG 1 cut(s) 2838
Bsp119I TTCGAA 1 cut(s) 1275
Bsp1286I GDGCHC 6 cut(s) 308, 692, 1533, 1724, 1822, 2845
Bsp13I TCCGGA 1 cut(s) 1932
Bsp1407I TGTACA 1 cut(s) 1761
Bsp1720I GCTNAGC 1 cut(s) 2337
BspANI GGCC 7 cut(s) 8, 182, 202, 1061, 1152, 1647, 1974
BspCNI CTCAG 5 cut(s) 310, 1105, 1672, 1853, 2329
BspEI TCCGGA 1 cut(s) 1932
BspFNI CGCG 5 cut(s) 36, 213, 265, 271, 420
BspLI GGNNCC 7 cut(s) 43, 160, 291, 657, 1298, 2028, 2882
BspMAI CTGCAG 1 cut(s) 1483
BspMI ACCTGC 1 cut(s) 242
BspOI GCTAGC 1 cut(s) 2088
BspPI GGATC 9 cut(s) 378, 423, 457, 650, 663, 735, 2021, 2034, 2868
BspQI GCTCTTC 1 cut(s) 2319
BspT104I TTCGAA 1 cut(s) 1275
BspT107I GGYRCC 2 cut(s) 41, 289
BspTI CTTAAG 1 cut(s) 2459
BspTNI GGTCTC 1 cut(s) 367
BsrBI CCGCTC 3 cut(s) 355, 614, 2312
BsrDI GCAATG 1 cut(s) 2175
BsrFI RCCGGY 1 cut(s) 44
BsrGI TGTACA 1 cut(s) 1761
BsrI ACTGG 5 cut(s) 243, 1666, 1824, 2452, 2757
BssAI RCCGGY 1 cut(s) 44
BssECI CCNNGG 6 cut(s) 34, 155, 957, 975, 1392, 2521
BssNI GRCGYC 2 cut(s) 42, 290
BssSI CACGAG 2 cut(s) 914, 2502
BssT1I CCWWGG 3 cut(s) 975, 1392, 2521
Bst2BI CACGAG 2 cut(s) 914, 2502
Bst2UI CCWGG 1 cut(s) 156
Bst4CI ACNGT 8 cut(s) 1202, 1253, 1745, 1805, 1966, 2642, 2791, 2874
Bst6I CTCTTC 4 cut(s) 156, 491, 1329, 2319
BstACI GRCGYC 2 cut(s) 42, 290
BstAFI CTTAAG 1 cut(s) 2459
BstAUI TGTACA 1 cut(s) 1761
BstBI TTCGAA 1 cut(s) 1275
BstC8I GCNNGC 7 cut(s) 19, 46, 924, 1870, 1926, 1979, 2086
BstDEI CTNAG 6 cut(s) 318, 1113, 1176, 1680, 1840, 2337
BstDSI CCRYGG 1 cut(s) 34
BstFNI CGCG 5 cut(s) 36, 213, 265, 271, 420
BstH2I RGCGCY 3 cut(s) 45, 135, 293
BstHHI GCGC 4 cut(s) 44, 134, 251, 292
BstMAI GTCTC 5 cut(s) 367, 1060, 1513, 1794, 2080
BstMCI CGRYCG 1 cut(s) 883
BstMWI GCNNNNNNNGC 9 cut(s) 14, 45, 50, 97, 173, 233, 255, 1826, 1865
BstNI CCWGG 1 cut(s) 156
BstNSI RCATGY 1 cut(s) 1073
BstPAI GACNNNNGTC 1 cut(s) 2769
BstSCI CCNGG 5 cut(s) 154, 171, 382, 658, 2865
BstSFI CTRYAG 2 cut(s) 1479, 1776
BstSLI GKGCMC 2 cut(s) 1724, 2845
BstUI CGCG 5 cut(s) 36, 213, 265, 271, 420
BstV1I GCAGC 5 cut(s) 65, 151, 1465, 1846, 2513
BstV2I GAAGAC 3 cut(s) 977, 2509, 2553
BstX2I RGATCY 2 cut(s) 655, 2026
BstYI RGATCY 2 cut(s) 655, 2026
BsuI GTATCC 1 cut(s) 1609
BsuRI GGCC 7 cut(s) 8, 182, 202, 1061, 1152, 1647, 1974
BtgI CCRYGG 1 cut(s) 34
BtrI CACGTC 1 cut(s) 1430
BtsI GCAGTG 2 cut(s) 1473, 2198
BtsIMutI CAGTG 9 cut(s) 250, 1207, 1473, 1831, 1971, 2198, 2328, 2764, 2870
BveI ACCTGC 1 cut(s) 242
Cac8I GCNNGC 7 cut(s) 19, 46, 924, 1870, 1926, 1979, 2086
CfoI GCGC 4 cut(s) 44, 134, 251, 292
Cfr10I RCCGGY 1 cut(s) 44
Cfr13I GGNCC 8 cut(s) 159, 181, 201, 276, 1150, 1396, 2063, 2795
Cfr42I CCGCGG 1 cut(s) 37
CseI GACGC 1 cut(s) 202
Csp6I GTAC 4 cut(s) 124, 1110, 1762, 2406
CspCI CAANNNNNGTGG 2 cut(s) 532, 567
CviAII CATG 9 cut(s) 280, 1063, 1070, 1292, 1675, 1899, 1997, 2318, 2689
CviQI GTAC 4 cut(s) 124, 1110, 1762, 2406
DdeI CTNAG 6 cut(s) 318, 1113, 1176, 1680, 1840, 2337
DinI GGCGCC 2 cut(s) 43, 291
EaeI YGGCCR 2 cut(s) 6, 1972
Eam1104I CTCTTC 4 cut(s) 156, 491, 1329, 2319
EarI CTCTTC 4 cut(s) 156, 491, 1329, 2319
Ecl136II GAGCTC 3 cut(s) 306, 690, 1531
Eco130I CCWWGG 3 cut(s) 975, 1392, 2521
Eco24I GRGCYC 3 cut(s) 308, 692, 1533
Eco31I GGTCTC 1 cut(s) 367
Eco32I GATATC 1 cut(s) 541
Eco47I GGWCC 5 cut(s) 159, 276, 1396, 2063, 2795
Eco53kI GAGCTC 3 cut(s) 306, 690, 1531
Eco57I CTGAAG 2 cut(s) 812, 2244
Eco88I CYCGRG 1 cut(s) 2838
EcoICRI GAGCTC 3 cut(s) 306, 690, 1531
EcoRI GAATTC 2 cut(s) 631, 2674
EcoRII CCWGG 1 cut(s) 154
EcoRV GATATC 1 cut(s) 541
EcoT14I CCWWGG 3 cut(s) 975, 1392, 2521
EcoT38I GRGCYC 3 cut(s) 308, 692, 1533
EgeI GGCGCC 2 cut(s) 43, 291
EheI GGCGCC 2 cut(s) 43, 291
ErhI CCWWGG 3 cut(s) 975, 1392, 2521
FaeI CATG 9 cut(s) 283, 1066, 1073, 1295, 1678, 1902, 2000, 2321, 2692
FalI AAGNNNNNCTT 2 cut(s) 1993, 2025
FaqI GGGAC 2 cut(s) 172, 801
FatI CATG 9 cut(s) 279, 1062, 1069, 1291, 1674, 1898, 1996, 2317, 2688
FauI CCCGC 1 cut(s) 73
FbaI TGATCA 1 cut(s) 1570
FblI GTMKAC 1 cut(s) 216
FriOI GRGCYC 3 cut(s) 308, 692, 1533
GlaI GCGC 4 cut(s) 43, 133, 250, 291
GsuI CTGGAG 2 cut(s) 615, 1683
HaeII RGCGCY 3 cut(s) 45, 135, 293
HaeIII GGCC 7 cut(s) 8, 182, 202, 1061, 1152, 1647, 1974
HapII CCGG 8 cut(s) 45, 135, 172, 199, 383, 659, 1933, 2867
HgaI GACGC 1 cut(s) 202
HhaI GCGC 4 cut(s) 44, 134, 251, 292
Hin1I GRCGYC 2 cut(s) 42, 290
Hin1II CATG 9 cut(s) 283, 1066, 1073, 1295, 1678, 1902, 2000, 2321, 2692
Hin6I GCGC 4 cut(s) 42, 132, 249, 290
HinP1I GCGC 4 cut(s) 42, 132, 249, 290
HincII GTYRAC 2 cut(s) 217, 2754
HindII GTYRAC 2 cut(s) 217, 2754
HindIII AAGCTT 3 cut(s) 908, 920, 2271
HpaII CCGG 8 cut(s) 45, 135, 172, 199, 383, 659, 1933, 2867
HphI GGTGA 5 cut(s) 788, 815, 1027, 1776, 2138
Hpy166II GTNNAC 8 cut(s) 217, 324, 1722, 1741, 1762, 2063, 2254, 2754
Hpy8I GTNNAC 8 cut(s) 217, 324, 1722, 1741, 1762, 2063, 2254, 2754
Hpy99I CGWCG 2 cut(s) 218, 1012
HpyCH4III ACNGT 8 cut(s) 1202, 1253, 1745, 1805, 1966, 2642, 2791, 2874
HpyCH4IV ACGT 3 cut(s) 126, 1007, 1429
HpyF10VI GCNNNNNNNGC 9 cut(s) 14, 45, 50, 97, 173, 233, 255, 1826, 1865
HpyF3I CTNAG 6 cut(s) 318, 1113, 1176, 1680, 1840, 2337
HpySE526I ACGT 3 cut(s) 126, 1007, 1429
Hsp92I GRCGYC 2 cut(s) 42, 290
Hsp92II CATG 9 cut(s) 283, 1066, 1073, 1295, 1678, 1902, 2000, 2321, 2692
HspAI GCGC 4 cut(s) 42, 132, 249, 290
KasI GGCGCC 2 cut(s) 41, 289
Kpn2I TCCGGA 1 cut(s) 1932
KroI GCCGGC 1 cut(s) 44
KroNI GCCGGC 1 cut(s) 46
Ksp22I TGATCA 1 cut(s) 1570
KspI CCGCGG 1 cut(s) 37
LguI GCTCTTC 1 cut(s) 2319
LmnI GCTCC 6 cut(s) 303, 352, 512, 695, 1528, 1825
Lsp1109I GCAGC 5 cut(s) 65, 151, 1465, 1846, 2513
MaeII ACGT 3 cut(s) 126, 1007, 1429
MaeIII GTNAC 3 cut(s) 1121, 1471, 1625
MbiI CCGCTC 3 cut(s) 355, 614, 2312
MfeI CAATTG 1 cut(s) 1368
MflI RGATCY 2 cut(s) 655, 2026
MhlI GDGCHC 6 cut(s) 308, 692, 1533, 1724, 1822, 2845
MluI ACGCGT 1 cut(s) 263
Mly113I GGCGCC 2 cut(s) 42, 290
MlyI GAGTC 3 cut(s) 806, 1697, 2779
MmeI TCCRAC 4 cut(s) 138, 1787, 2279, 2468
MreI CGCCGGCG 1 cut(s) 44
MroI TCCGGA 1 cut(s) 1932
MroNI GCCGGC 1 cut(s) 44
MseI TTAA 6 cut(s) 945, 966, 1245, 2262, 2421, 2460
MslI CAYNNNNRTG 1 cut(s) 1074
MspA1I CMGCKG 4 cut(s) 36, 164, 1633, 1868
MspCI CTTAAG 1 cut(s) 2459
MspI CCGG 8 cut(s) 45, 135, 172, 199, 383, 659, 1933, 2867
MspR9I CCNGG 5 cut(s) 156, 173, 384, 660, 2867
MunI CAATTG 1 cut(s) 1368
Mva1269I GAATGC 1 cut(s) 589
MvaI CCWGG 1 cut(s) 156
MvnI CGCG 5 cut(s) 36, 213, 265, 271, 420
MwoI GCNNNNNNNGC 9 cut(s) 14, 45, 50, 97, 173, 233, 255, 1826, 1865
NaeI GCCGGC 1 cut(s) 46
NarI GGCGCC 2 cut(s) 42, 290
NciI CCSGG 4 cut(s) 173, 384, 660, 2867
NgoMIV GCCGGC 1 cut(s) 44
NheI GCTAGC 1 cut(s) 2084
NlaIII CATG 9 cut(s) 283, 1066, 1073, 1295, 1678, 1902, 2000, 2321, 2692
NlaIV GGNNCC 7 cut(s) 43, 160, 291, 657, 1298, 2028, 2882
NmeAIII GCCGAG 1 cut(s) 318
NmuCI GTSAC 2 cut(s) 1121, 1471
NspI RCATGY 1 cut(s) 1073
NspV TTCGAA 1 cut(s) 1275
PciI ACATGT 1 cut(s) 1069
PciSI GCTCTTC 1 cut(s) 2319
PctI GAATGC 1 cut(s) 589
PdiI GCCGGC 1 cut(s) 46
PfeI GAWTC 7 cut(s) 1079, 1268, 2106, 2414, 2510, 2627, 2834
PfoI TCCNGGA 1 cut(s) 658
PleI GAGTC 3 cut(s) 805, 1696, 2778
PluTI GGCGCC 2 cut(s) 45, 293
PpsI GAGTC 3 cut(s) 805, 1696, 2778
PscI ACATGT 1 cut(s) 1069
PshAI GACNNNNGTC 1 cut(s) 2769
Psp124BI GAGCTC 3 cut(s) 308, 692, 1533
Psp6I CCWGG 1 cut(s) 154
PspGI CCWGG 1 cut(s) 154
PspN4I GGNNCC 7 cut(s) 43, 160, 291, 657, 1298, 2028, 2882
PspPI GGNCC 8 cut(s) 159, 181, 201, 276, 1150, 1396, 2063, 2795
PstI CTGCAG 1 cut(s) 1483
PsuI RGATCY 2 cut(s) 655, 2026
PvuII CAGCTG 2 cut(s) 1633, 1868
RsaI GTAC 4 cut(s) 125, 1111, 1763, 2407
RsaNI GTAC 4 cut(s) 124, 1110, 1762, 2406
RseI CAYNNNNRTG 1 cut(s) 1074
SacI GAGCTC 3 cut(s) 308, 692, 1533
SacII CCGCGG 1 cut(s) 37
SalI GTCGAC 1 cut(s) 215
SapI GCTCTTC 1 cut(s) 2319
SaqAI TTAA 6 cut(s) 945, 966, 1245, 2262, 2421, 2460
Sau96I GGNCC 8 cut(s) 159, 181, 201, 276, 1150, 1396, 2063, 2795
ScaI AGTACT 1 cut(s) 1111
SchI GAGTC 3 cut(s) 806, 1697, 2779
ScrFI CCNGG 5 cut(s) 156, 173, 384, 660, 2867
SduI GDGCHC 6 cut(s) 308, 692, 1533, 1724, 1822, 2845
SfcI CTRYAG 2 cut(s) 1479, 1776
SfoI GGCGCC 2 cut(s) 43, 291
Sfr303I CCGCGG 1 cut(s) 37
SfuI TTCGAA 1 cut(s) 1275
SgrAI CRCCGGYG 1 cut(s) 44
SgrBI CCGCGG 1 cut(s) 37
SinI GGWCC 5 cut(s) 159, 276, 1396, 2063, 2795
SmiMI CAYNNNNRTG 1 cut(s) 1074
SmlI CTYRAG 4 cut(s) 2268, 2409, 2459, 2854
SmoI CTYRAG 4 cut(s) 2268, 2409, 2459, 2854
SpeI ACTAGT 2 cut(s) 1313, 2531
SspDI GGCGCC 2 cut(s) 41, 289
SstI GAGCTC 3 cut(s) 308, 692, 1533
StyD4I CCNGG 5 cut(s) 154, 171, 382, 658, 2865
StyI CCWWGG 3 cut(s) 975, 1392, 2521
TaaI ACNGT 8 cut(s) 1202, 1253, 1745, 1805, 1966, 2642, 2791, 2874
TaiI ACGT 3 cut(s) 129, 1010, 1432
TaqI TCGA 5 cut(s) 216, 428, 1010, 1275, 2356
TatI WGTACW 3 cut(s) 1109, 1761, 2405
TauI GCSGC 7 cut(s) 24, 39, 42, 71, 227, 239, 1977
TfiI GAWTC 7 cut(s) 1079, 1268, 2106, 2414, 2510, 2627, 2834
Tru1I TTAA 6 cut(s) 945, 966, 1245, 2262, 2421, 2460
Tru9I TTAA 6 cut(s) 945, 966, 1245, 2262, 2421, 2460
TscAI CASTG 9 cut(s) 250, 1207, 1480, 1831, 1971, 2198, 2335, 2764, 2877
TseFI GTSAC 2 cut(s) 1121, 1471
TseI GCWGC 5 cut(s) 53, 164, 1478, 1859, 2526
Tsp45I GTSAC 2 cut(s) 1121, 1471
TspDTI ATGAA 7 cut(s) 876, 1007, 1563, 1663, 1887, 2153, 2306
TspGWI ACGGA 4 cut(s) 1440, 1805, 2108, 2754
TspRI CASTG 9 cut(s) 250, 1207, 1480, 1831, 1971, 2198, 2335, 2764, 2877
Vha464I CTTAAG 1 cut(s) 2459
VneI GTGCAC 1 cut(s) 1720
VpaK11BI GGWCC 5 cut(s) 159, 276, 1396, 2063, 2795
XapI RAATTY 7 cut(s) 568, 631, 733, 1502, 1522, 2304, 2674
XceI RCATGY 1 cut(s) 1073
XcmI CCANNNNNNNNNTGG 1 cut(s) 982
XmiI GTMKAC 1 cut(s) 216
ZrmI AGTACT 1 cut(s) 1111
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.