Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0008343

Physical Location & Seq

Genomic Coordinates Forward (+)

22131076 .. 22140057

Gene Structure 8982 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1226600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2589 bp

ATGGCGTCTCTGCAAACCCACCATTACCATCTTCAACTCCACAGCCGCTGCGTCTCCTCTCTCACTGCCCCCAAACTCTGCAACCTTCGATTCCCCAACCTTTCTCTGCGCTTCCCGGCTTCATTGCCCGGGAAAGTTAGGGTTTTTACTCTCAGAGGAAATGGGAGGGGCAAGGATGGAATTTACTCGTTTTGCTGCGTTTGCAGAGCTGGTGCTGAGATTGAAAAGGTTGATGGTGAGGAAGGAAGTGAAAGGCCTCCTTTTGATATTAATTTGGCCGTGGTTCTTGCCGGTTTTGCTTTCGAGGCCTACTCGAGCCCTCCTGATAATGTTGGAAGGCATGAAGTTGATGCTGCAGATTGTAAGACAGTGTATCTTTCAGAGTCATTTATACGTGAGATCTATGATGGCGAATTATCTGTTAAACTGAAAAAAGGTCTCGAGTTACCAGCCATGGATCCATGGGGAACAAGTGATCCATATGTAATTATGACATTAGATGGTCAAGTCGTAAAAAGCAAGGTTAAATGGGGGACAAAAGAGCCAACATGGAACGAGGACTTCAGTTTTAATATCAAGCTGCCTGCTACCAAAAATCTTCAGGTTGCAGCTTGGGATGCAAACCTTGTAAGTCCACATAAACGAATGGGGAATGCGAGCATCAGTCTAGAAGACCTTTGTGATGGAAATTTGCATGAAGTAGTGGTGGAATTGGAAGGAATGGGAGGCGGTGGAAAACTACTTTTAGAGGTCAACTATAAAACTTTTGATGAAATTGATGAGGCAAAAAAGTGGTGGAGTAGGGTCCCCTTTGTTTCCGACTTTCTCCGTAAAAATGGTTTTGAGCCTGCTATGAAAAAAATGTTTGCTGGCTCAGACACTGTGCAAGCACGTCAATTTGTGGAATATGCTTTTGGGCAGCTAAAGTCATTCAATAATGCAAACCTTATGAAGAATTTAATTGCAAGCGGTGATGTGAATGACACGAAAGGCACTGGGAAATCTATTTCTGCTGGTGTGTCAGATGTGACTTCCCAGATGGATACTATAGCAGAAGGTTTTTTAGATAATGCAGGTTTCAATGCAAGCAGCAATGTAGACGAGGCCAGCATAGATAATGGTGGTGTGGAGCATGGTGAAGAAACACAATCAGATAAGAATTTCTGGAAGAAATTTGCCAATGAAATCAATCAAAATGTTGCTGAGAAATTTGGTCTGCCAGTTCCAGAGAAATTAAAGTGGGATGGGTTTGATATATTAAACAGATTTGGTTCGCAGTCACGAGAGATAGCAGAAGCATCTTATATTGAATCAGGGCTTGCAACTCCAGAAGGCTTAGATGTTGATAAGGATAAAACAACTAGCCCACTTTCCATTAGCATGATTCAGTCTTCACTTCCAGATATTAAGAAAGCAACAAGGGATCTATTAAAGCAAACTGATTCTGTTTTAGGAACATTTGTGGTTCTAACTGCTGCAGTATCTGAATCAAACACGGAATCAAATGCAGTAGGAACGAGTGAAATTAAATTAGAGGATAGTAGCAATGTAGAAGATGATGCCTTGACTGATCCCACAACCGAGGAGATTGCTAGCACACGGGCTGCAGAGGAGATGAAAGAACTCTTTTCTAGTGCAGAAAGTGCCATGGAGGCTTGGGCAATGCTTGCTACCTCTTTGGGACACCCAAGTTTCATAAAGTCCGAATTTGAGAAACTATGTTTCTTAGATAATGCAACAACAGACACACAGGTGGCAATTTGGCGTGATTCTGCTCGGAAAAGACTAGTTATTGCTTTCAGGGGAACAGAACAGTCAAGATGGAAGGACTTGCGAACAGATCTGATGGTAGCTCCTACTGGGCTAAATCCAGAAAGAATAGGCGGAGACTTCAAACAAGAAGTTCAAGTTCATAGTGGTTTCTTAAGGGCATATGATTCTGTCAGGATAAGGATCGTCTCTCTCATGAAATTGGCAATTGGTTATTTTGATGATATTGCTGAGCCGCTGGACAAATGGCATGTTTATATAACGGGTCATAGTCTAGGTGGTGCATTGTCGACCCTCCTGGCTCTTGAACTTTCCTCAAGTCAGCTAGCAAAGCGTGGACTGATTTCTGTGACCATGTATAACTTCGGATCTCCTAGAGTTGGTAACAAAGTATTTGCCGAAATTTATAACAAGAAAGTCAAAGATAGCTGGAGAGTTGTAAACCACAGAGACATTATCCCTACAATCCCCCGCCTGATGGGATACTGCCATGTAGCTCAACCTGTTTACCTAACAACAGGAGACCTAAAAAATGCCTTGGGAAATATGGAGCTCTCTGAAGATGGTTACCAAGCTGATGTGATTGGGGAGTACACACCAGATGTACTTGTCGGTGAATTCATGAAGGGTGAGAAGGAGCTTATTGAGAAAATATTACAGACCGAAATAAACATATTCCGTTCAGTCAGAGATGGGACTGCACTTATGCAGCACATGGAGGATTTTTACTACATCACACTACTAGAGAATGTGAGATCGAATTACCAAGTCGCAGCAAGGACGCTGTCCGAAGAACAAGCGAATGTGAAAGTTAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001505 GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005783 GO:0005789 GO:0007154 GO:0007165 GO:0008021 GO:0008150 GO:0009987 GO:0010646 GO:0012505 GO:0012506 GO:0016020 GO:0016021 GO:0019722 GO:0019932 GO:0023051 GO:0023052 GO:0030100 GO:0030133 GO:0030334 GO:0030336 GO:0030658 GO:0030659 GO:0030672 GO:0031090 GO:0031224 GO:0031410 GO:0031982 GO:0031984 GO:0032879 GO:0035556 GO:0040012 GO:0040013 GO:0042175 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044433 GO:0044444 GO:0044446 GO:0044456 GO:0044464 GO:0045202 GO:0045806 GO:0046872 GO:0046928 GO:0048167 GO:0048168 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050804 GO:0050896 GO:0051046 GO:0051049 GO:0051051 GO:0051128 GO:0051129 GO:0051270 GO:0051271 GO:0051588 GO:0051716 GO:0055037 GO:0060341 GO:0060627 GO:0065007 GO:0065008 GO:0070382 GO:0080134 GO:0097458 GO:0097708 GO:0098588 GO:0098793 GO:0098805 GO:0098827 GO:0099177 GO:0099501 GO:0099503 GO:1902882 GO:1902883 GO:1903530 GO:2000145 GO:2000146

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

C2 Lipase_3

Protein Analysis

863

Amino Acids

95.42

Weight (kDa)

5.13

Isoelectric Point (pI)

37.36

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	137 - 233	2.6e-19	C2 domain
Lipase_3	PF01764	597 - 748	5.5e-28	Lipase (class 3)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008343)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13550 AT4G13550 AT4G13550
fragaria_vesca	FvH4_4g05250 FvH4_4g05250
malus_domestica	MD13G1226600.v1.1
prunus_persica	Prupe.1G054000_v2.0.a1 Prupe.1G054000_v2.0.a1 Prupe.1G054000_v2.0.a1 Prupe.1G054000_v2.0.a1 Prupe.1G054000_v2.0.a1
pyrus_communis	pycom13g19960
rosa_chinensis	RchiOBHm_Chr4g0396161
rosa_laevigata	RLG00000009564
rosa_multiflora	Rmu_sc0006325.1_g000041
rosa_roxburghii	Rroxscaffold_5G00341010
rosa_rugosa	Rorug03G0351200 Rorug03G0351300 Rorug03G0351300
rosa_samantha	Rh4BG065200 Rh4CG072000 Rh4DG062000
rosa_wichuraiana	Rw4G005390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	2178
Acc36I	ACCTGC	1 cut(s)	1064
AccI	GTMKAC	2 cut(s)	1098, 2060
AciI	CCGC	6 cut(s)	46, 729, 969, 1884, 2006, 2242
AclWI	GGATC	7 cut(s)	452, 465, 470, 1431, 1565, 1961, 2146
AcoI	YGGCCR	1 cut(s)	276
AcsI	RAATTY	9 cut(s)	180, 688, 955, 1159, 1172, 1208, 1706, 2172, 2387
AcuI	CTGAAG	3 cut(s)	547, 584, 2349
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	2 cut(s)	2363, 2376
AfiI	CCNNNNNNNGG	2 cut(s)	2150, 2248
AflII	CTTAAG	1 cut(s)	1924
AgsI	TTSAA	8 cut(s)	35, 224, 934, 1081, 1310, 1894, 1907, 2078
AhlI	ACTAGT	1 cut(s)	1786
AjiI	CACGTC	1 cut(s)	893
AjnI	CCWGG	1 cut(s)	2067
AjuI	GAANNNNNNNTTGG	2 cut(s)	897, 929
AleI	CACNNNNGTG	1 cut(s)	1751
AloI	GAACNNNNNNTCC	2 cut(s)	460, 492
Alw21I	GWGCWC	1 cut(s)	2325
Alw26I	GTCTC	7 cut(s)	12, 58, 443, 1881, 1963, 2214, 2286
AlwI	GGATC	7 cut(s)	452, 465, 470, 1431, 1565, 1961, 2146
AlwNI	CAGNNNCTG	3 cut(s)	48, 881, 1484
Ama87I	CYCGRG	3 cut(s)	128, 313, 440
AoxI	GGCC	4 cut(s)	254, 276, 306, 1104
ApoI	RAATTY	9 cut(s)	180, 688, 955, 1159, 1172, 1208, 1706, 2172, 2387
AseI	ATTAAT	1 cut(s)	270
AspLEI	GCGC	1 cut(s)	111
AspS9I	GGNCC	1 cut(s)	805
AsuC2I	CCSGG	3 cut(s)	116, 129, 130
AsuHPI	GGTGA	5 cut(s)	248, 983, 1148, 2396, 2411
AsuNHI	GCTAGC	2 cut(s)	1592, 2095
AvaI	CYCGRG	3 cut(s)	128, 313, 440
AvaII	GGWCC	1 cut(s)	805
BamHI	GGATCC	1 cut(s)	457
BanII	GRGCYC	2 cut(s)	320, 2325
BauI	CACGAG	1 cut(s)	1281
BbsI	GAAGAC	2 cut(s)	678, 1383
Bbv12I	GWGCWC	1 cut(s)	2325
BceAI	ACGGC	1 cut(s)	263
BcgI	CGANNNNNNTGC	2 cut(s)	1571, 1605
BciT130I	CCWGG	1 cut(s)	2069
BciVI	GTATCC	2 cut(s)	1036, 2246
BcnI	CCSGG	3 cut(s)	116, 129, 130
BcoDI	GTCTC	7 cut(s)	12, 58, 443, 1881, 1963, 2214, 2286
BcuI	ACTAGT	1 cut(s)	1786
BfaI	CTAG	9 cut(s)	668, 1362, 1593, 1632, 1787, 2045, 2096, 2145, 2513
BfmI	CTRYAG	4 cut(s)	354, 1047, 1476, 1605
BfrI	CTTAAG	1 cut(s)	1924
BfuAI	ACCTGC	1 cut(s)	1064
BfuI	GTATCC	2 cut(s)	1036, 2246
BglI	GCCNNNNNGGC	1 cut(s)	1652
BglII	AGATCT	2 cut(s)	399, 1840
BlpI	GCTNAGC	1 cut(s)	2001
Bme1390I	CCNGG	4 cut(s)	116, 129, 130, 2069
Bme18I	GGWCC	1 cut(s)	805
BmeT110I	CYCGRG	3 cut(s)	128, 313, 440
BmgBI	CACGTC	1 cut(s)	893
BmgT120I	GGNCC	1 cut(s)	805
BmiI	GGNNCC	3 cut(s)	459, 806, 807
BmrFI	CCNGG	4 cut(s)	116, 129, 130, 2069
BmrI	ACTGGG	2 cut(s)	1005, 1869
BmsI	GCATC	5 cut(s)	340, 607, 669, 1307, 1549
BmtI	GCTAGC	2 cut(s)	1596, 2099
BmuI	ACTGGG	2 cut(s)	1005, 1869
BpiI	GAAGAC	2 cut(s)	678, 1383
BplI	GAGNNNNNCTC	2 cut(s)	296, 328
BpmI	CTGGAG	2 cut(s)	1311, 2221
Bpu1102I	GCTNAGC	1 cut(s)	2001
BpuEI	CTTGAG	1 cut(s)	2071
BpuMI	CCSGG	3 cut(s)	116, 129, 130
BsaAI	YACGTR	1 cut(s)	395
BsaBI	GATNNNNATC	1 cut(s)	1952
BsaHI	GRCGYC	1 cut(s)	5
BsaI	GGTCTC	2 cut(s)	443, 2286
BsaJI	CCNNGG	7 cut(s)	128, 279, 453, 461, 1581, 1647, 2307
Bsc4I	CCNNNNNNNGG	2 cut(s)	2150, 2248
Bse118I	RCCGGY	1 cut(s)	290
Bse1I	ACTGG	3 cut(s)	1000, 1220, 1864
Bse3DI	GCAATG	4 cut(s)	122, 1099, 1552, 1668
Bse8I	GATNNNNATC	1 cut(s)	1952
BseBI	CCWGG	1 cut(s)	2069
BseDI	CCNNGG	7 cut(s)	128, 279, 453, 461, 1581, 1647, 2307
BseGI	GGATG	3 cut(s)	181, 622, 1249
BseJI	GATNNNNATC	1 cut(s)	1952
BseLI	CCNNNNNNNGG	2 cut(s)	2150, 2248
BseMI	GCAATG	4 cut(s)	122, 1099, 1552, 1668
BseMII	CTCAG	5 cut(s)	166, 207, 888, 1194, 1992
BseNI	ACTGG	3 cut(s)	1000, 1220, 1864
BseRI	GAGGAG	3 cut(s)	46, 1598, 1625
BsgI	GTGCAG	2 cut(s)	1656, 2454
BshFI	GGCC	4 cut(s)	256, 278, 308, 1106
BsiHKAI	GWGCWC	1 cut(s)	2325
BsiHKCI	CYCGRG	3 cut(s)	128, 313, 440
BsiSI	CCGG	3 cut(s)	116, 129, 291
BslFI	GGGAC	4 cut(s)	547, 791, 1695, 2479
BslI	CCNNNNNNNGG	2 cut(s)	2150, 2248
BsmAI	GTCTC	7 cut(s)	12, 58, 443, 1881, 1963, 2214, 2286
BsmBI	CGTCTC	3 cut(s)	12, 58, 1963
BsmFI	GGGAC	4 cut(s)	547, 791, 1695, 2479
BsmI	GAATGC	1 cut(s)	658
BsnI	GGCC	4 cut(s)	256, 278, 308, 1106
Bso31I	GGTCTC	2 cut(s)	443, 2286
BsoBI	CYCGRG	3 cut(s)	128, 313, 440
Bsp1286I	GDGCHC	2 cut(s)	320, 2325
Bsp143I	GATC	9 cut(s)	399, 457, 475, 1423, 1570, 1840, 1953, 2138, 2525
Bsp1720I	GCTNAGC	1 cut(s)	2001
Bsp19I	CCATGG	3 cut(s)	453, 461, 1647
BspACI	CCGC	6 cut(s)	46, 729, 969, 1884, 2006, 2242
BspANI	GGCC	4 cut(s)	256, 278, 308, 1106
BspCNI	CTCAG	5 cut(s)	165, 208, 887, 1195, 1993
BspHI	TCATGA	2 cut(s)	1965, 2391
BspLI	GGNNCC	3 cut(s)	459, 806, 807
BspMAI	CTGCAG	3 cut(s)	358, 1480, 1609
BspMI	ACCTGC	1 cut(s)	1064
BspOI	GCTAGC	2 cut(s)	1596, 2099
BspPI	GGATC	7 cut(s)	452, 465, 470, 1431, 1565, 1961, 2146
BspTI	CTTAAG	1 cut(s)	1924
BspTNI	GGTCTC	2 cut(s)	443, 2286
BsrDI	GCAATG	4 cut(s)	122, 1099, 1552, 1668
BsrFI	RCCGGY	1 cut(s)	290
BsrI	ACTGG	3 cut(s)	1000, 1220, 1864
BssAI	RCCGGY	1 cut(s)	290
BssECI	CCNNGG	7 cut(s)	128, 279, 453, 461, 1581, 1647, 2307
BssMI	GATC	9 cut(s)	399, 457, 475, 1423, 1570, 1840, 1953, 2138, 2525
BssNI	GRCGYC	1 cut(s)	5
BssSI	CACGAG	1 cut(s)	1281
BssT1I	CCWWGG	4 cut(s)	453, 461, 1647, 2307
Bst2BI	CACGAG	1 cut(s)	1281
Bst2UI	CCWGG	1 cut(s)	2069
Bst4CI	ACNGT	3 cut(s)	370, 883, 1815
BstACI	GRCGYC	1 cut(s)	5
BstAFI	CTTAAG	1 cut(s)	1924
BstAPI	GCANNNNNTGC	2 cut(s)	1643, 1667
BstBAI	YACGTR	1 cut(s)	395
BstDEI	CTNAG	7 cut(s)	152, 216, 874, 1203, 1336, 1726, 2001
BstDSI	CCRYGG	4 cut(s)	279, 453, 461, 1647
BstEII	GGTNACC	1 cut(s)	2336
BstF5I	GGATG	3 cut(s)	181, 622, 1249
BstHHI	GCGC	1 cut(s)	111
BstKTI	GATC	9 cut(s)	402, 460, 478, 1426, 1573, 1843, 1956, 2141, 2528
BstMAI	GTCTC	7 cut(s)	12, 58, 443, 1881, 1963, 2214, 2286
BstMBI	GATC	9 cut(s)	399, 457, 475, 1423, 1570, 1840, 1953, 2138, 2525
BstMWI	GCNNNNNNNGC	8 cut(s)	201, 296, 305, 617, 1643, 1652, 1667, 2101
BstNI	CCWGG	1 cut(s)	2069
BstNSI	RCATGY	1 cut(s)	2024
BstPI	GGTNACC	1 cut(s)	2336
BstSCI	CCNGG	4 cut(s)	114, 127, 128, 2067
BstSFI	CTRYAG	4 cut(s)	354, 1047, 1476, 1605
BstV2I	GAAGAC	2 cut(s)	678, 1383
BstX2I	RGATCY	5 cut(s)	399, 457, 1423, 1840, 2138
BstYI	RGATCY	5 cut(s)	399, 457, 1423, 1840, 2138
BsuI	GTATCC	2 cut(s)	1036, 2246
BsuRI	GGCC	4 cut(s)	256, 278, 308, 1106
BtgI	CCRYGG	4 cut(s)	279, 453, 461, 1647
BtrI	CACGTC	1 cut(s)	893
BtsCI	GGATG	3 cut(s)	181, 622, 1249
BtsI	GCAGTG	1 cut(s)	63
BtsIMutI	CAGTG	4 cut(s)	63, 375, 879, 993
BveI	ACCTGC	1 cut(s)	1064
CaiI	CAGNNNCTG	3 cut(s)	48, 881, 1484
CciI	TCATGA	2 cut(s)	1965, 2391
CfoI	GCGC	1 cut(s)	111
Cfr10I	RCCGGY	1 cut(s)	290
Cfr13I	GGNCC	1 cut(s)	805
Cfr9I	CCCGGG	1 cut(s)	128
CseI	GACGC	2 cut(s)	40, 2560
Csp6I	GTAC	2 cut(s)	2362, 2375
CviQI	GTAC	2 cut(s)	2362, 2375
DdeI	CTNAG	7 cut(s)	152, 216, 874, 1203, 1336, 1726, 2001
DpnI	GATC	9 cut(s)	401, 459, 477, 1425, 1572, 1842, 1955, 2140, 2527
DpnII	GATC	9 cut(s)	399, 457, 475, 1423, 1570, 1840, 1953, 2138, 2525
EaeI	YGGCCR	1 cut(s)	276
EciI	GGCGGA	1 cut(s)	1899
Ecl136II	GAGCTC	1 cut(s)	2323
Eco130I	CCWWGG	4 cut(s)	453, 461, 1647, 2307
Eco147I	AGGCCT	2 cut(s)	256, 308
Eco24I	GRGCYC	2 cut(s)	320, 2325
Eco31I	GGTCTC	2 cut(s)	443, 2286
Eco47I	GGWCC	1 cut(s)	805
Eco53kI	GAGCTC	1 cut(s)	2323
Eco57I	CTGAAG	3 cut(s)	547, 584, 2349
Eco88I	CYCGRG	3 cut(s)	128, 313, 440
Eco91I	GGTNACC	1 cut(s)	2336
EcoICRI	GAGCTC	1 cut(s)	2323
EcoO109I	RGGNCCY	1 cut(s)	805
EcoO65I	GGTNACC	1 cut(s)	2336
EcoRI	GAATTC	1 cut(s)	2387
EcoRII	CCWGG	1 cut(s)	2067
EcoT14I	CCWWGG	4 cut(s)	453, 461, 1647, 2307
EcoT38I	GRGCYC	2 cut(s)	320, 2325
ErhI	CCWWGG	4 cut(s)	453, 461, 1647, 2307
Esp3I	CGTCTC	3 cut(s)	12, 58, 1963
FalI	AAGNNNNNCTT	2 cut(s)	244, 276
FaqI	GGGAC	4 cut(s)	547, 791, 1695, 2479
FauI	CCCGC	1 cut(s)	2249
FauNDI	CATATG	2 cut(s)	481, 1933
FblI	GTMKAC	2 cut(s)	1098, 2060
FokI	GGATG	3 cut(s)	188, 629, 1256
FriOI	GRGCYC	2 cut(s)	320, 2325
FspBI	CTAG	9 cut(s)	668, 1362, 1593, 1632, 1787, 2045, 2096, 2145, 2513
GlaI	GCGC	1 cut(s)	110
GsuI	CTGGAG	2 cut(s)	1311, 2221
HaeIII	GGCC	4 cut(s)	256, 278, 308, 1106
HapII	CCGG	3 cut(s)	116, 129, 291
HgaI	GACGC	2 cut(s)	40, 2560
HhaI	GCGC	1 cut(s)	111
Hin1I	GRCGYC	1 cut(s)	5
Hin6I	GCGC	1 cut(s)	109
HinP1I	GCGC	1 cut(s)	109
HincII	GTYRAC	2 cut(s)	754, 2061
HindII	GTYRAC	2 cut(s)	754, 2061
HinfI	GANTC	9 cut(s)	90, 383, 1310, 1384, 1442, 1487, 1499, 1769, 1937
HpaII	CCGG	3 cut(s)	116, 129, 291
HphI	GGTGA	5 cut(s)	248, 983, 1148, 2396, 2411
Hpy166II	GTNNAC	8 cut(s)	635, 754, 1099, 2061, 2108, 2212, 2278, 2364
Hpy8I	GTNNAC	8 cut(s)	635, 754, 1099, 2061, 2108, 2212, 2278, 2364
HpyAV	CCTTC	9 cut(s)	95, 236, 330, 710, 1049, 1325, 1819, 2389, 2398
HpyCH4III	ACNGT	3 cut(s)	370, 883, 1815
HpyCH4IV	ACGT	2 cut(s)	394, 892
HpyF10VI	GCNNNNNNNGC	8 cut(s)	201, 296, 305, 617, 1643, 1652, 1667, 2101
HpyF3I	CTNAG	7 cut(s)	152, 216, 874, 1203, 1336, 1726, 2001
HpySE526I	ACGT	2 cut(s)	394, 892
Hsp92I	GRCGYC	1 cut(s)	5
HspAI	GCGC	1 cut(s)	109
KflI	GGGWCCC	1 cut(s)	805
Kzo9I	GATC	9 cut(s)	399, 457, 475, 1423, 1570, 1840, 1953, 2138, 2525
LmnI	GCTCC	4 cut(s)	1129, 1858, 2320, 2407
LweI	GCATC	5 cut(s)	340, 607, 669, 1307, 1549
MaeI	CTAG	9 cut(s)	668, 1362, 1593, 1632, 1787, 2045, 2096, 2145, 2513
MaeII	ACGT	2 cut(s)	394, 892
MaeIII	GTNAC	6 cut(s)	444, 1027, 1278, 2119, 2153, 2336
MalI	GATC	9 cut(s)	401, 459, 477, 1425, 1572, 1842, 1955, 2140, 2527
MboI	GATC	9 cut(s)	399, 457, 475, 1423, 1570, 1840, 1953, 2138, 2525
MfeI	CAATTG	1 cut(s)	1977
MflI	RGATCY	5 cut(s)	399, 457, 1423, 1840, 2138
MhlI	GDGCHC	2 cut(s)	320, 2325
MlyI	GAGTC	1 cut(s)	392
MmeI	TCCRAC	2 cut(s)	313, 843
MslI	CAYNNNNRTG	2 cut(s)	1379, 1751
MspA1I	CMGCKG	2 cut(s)	48, 2008
MspCI	CTTAAG	1 cut(s)	1924
MspI	CCGG	3 cut(s)	116, 129, 291
MspR9I	CCNGG	4 cut(s)	116, 129, 130, 2069
MunI	CAATTG	1 cut(s)	1977
Mva1269I	GAATGC	1 cut(s)	658
MvaI	CCWGG	1 cut(s)	2069
MwoI	GCNNNNNNNGC	8 cut(s)	201, 296, 305, 617, 1643, 1652, 1667, 2101
NciI	CCSGG	3 cut(s)	116, 129, 130
NcoI	CCATGG	3 cut(s)	453, 461, 1647
NdeI	CATATG	2 cut(s)	481, 1933
NdeII	GATC	9 cut(s)	399, 457, 475, 1423, 1570, 1840, 1953, 2138, 2525
NheI	GCTAGC	2 cut(s)	1592, 2095
NlaIV	GGNNCC	3 cut(s)	459, 806, 807
NmuCI	GTSAC	3 cut(s)	1027, 1278, 2119
NspI	RCATGY	1 cut(s)	2024
OliI	CACNNNNGTG	1 cut(s)	1751
PaeR7I	CTCGAG	2 cut(s)	313, 440
PagI	TCATGA	2 cut(s)	1965, 2391
PceI	AGGCCT	2 cut(s)	256, 308
PctI	GAATGC	1 cut(s)	658
PfeI	GAWTC	8 cut(s)	90, 1310, 1384, 1442, 1487, 1499, 1769, 1937
PflFI	GACNNNGTC	1 cut(s)	2554
PleI	GAGTC	1 cut(s)	391
PpsI	GAGTC	1 cut(s)	391
Ppu21I	YACGTR	1 cut(s)	395
PpuMI	RGGWCCY	1 cut(s)	805
PshBI	ATTAAT	1 cut(s)	270
PsiI	TTATAA	1 cut(s)	2178
Psp124BI	GAGCTC	1 cut(s)	2325
Psp5II	RGGWCCY	1 cut(s)	805
Psp6I	CCWGG	1 cut(s)	2067
PspEI	GGTNACC	1 cut(s)	2336
PspGI	CCWGG	1 cut(s)	2067
PspN4I	GGNNCC	3 cut(s)	459, 806, 807
PspPI	GGNCC	1 cut(s)	805
PspPPI	RGGWCCY	1 cut(s)	805
PspXI	VCTCGAGB	1 cut(s)	313
PstI	CTGCAG	3 cut(s)	358, 1480, 1609
PstNI	CAGNNNCTG	3 cut(s)	48, 881, 1484
PsuI	RGATCY	5 cut(s)	399, 457, 1423, 1840, 2138
PsyI	GACNNNGTC	1 cut(s)	2554
RsaI	GTAC	2 cut(s)	2363, 2376
RsaNI	GTAC	2 cut(s)	2362, 2375
RseI	CAYNNNNRTG	2 cut(s)	1379, 1751
SacI	GAGCTC	1 cut(s)	2325
SalI	GTCGAC	1 cut(s)	2059
Sau3AI	GATC	9 cut(s)	399, 457, 475, 1423, 1570, 1840, 1953, 2138, 2525
Sau96I	GGNCC	1 cut(s)	805
SchI	GAGTC	1 cut(s)	392
ScrFI	CCNGG	4 cut(s)	116, 129, 130, 2069
SduI	GDGCHC	2 cut(s)	320, 2325
SfaNI	GCATC	5 cut(s)	340, 607, 669, 1307, 1549
SfcI	CTRYAG	4 cut(s)	354, 1047, 1476, 1605
Sfr274I	CTCGAG	2 cut(s)	313, 440
SinI	GGWCC	1 cut(s)	805
SlaI	CTCGAG	2 cut(s)	313, 440
SmaI	CCCGGG	1 cut(s)	130
SmiMI	CAYNNNNRTG	2 cut(s)	1379, 1751
SmlI	CTYRAG	4 cut(s)	313, 440, 1924, 2086
SmoI	CTYRAG	4 cut(s)	313, 440, 1924, 2086
SpeI	ACTAGT	1 cut(s)	1786
SseBI	AGGCCT	2 cut(s)	256, 308
SsiI	CCGC	6 cut(s)	46, 729, 969, 1884, 2006, 2242
SspI	AATATT	1 cut(s)	2424
SspMI	CTAG	9 cut(s)	668, 1362, 1593, 1632, 1787, 2045, 2096, 2145, 2513
SstI	GAGCTC	1 cut(s)	2325
StuI	AGGCCT	2 cut(s)	256, 308
StyD4I	CCNGG	4 cut(s)	114, 127, 128, 2067
StyI	CCWWGG	4 cut(s)	453, 461, 1647, 2307
TaaI	ACNGT	3 cut(s)	370, 883, 1815
TaiI	ACGT	2 cut(s)	397, 895
TaqI	TCGA	6 cut(s)	88, 303, 314, 441, 2060, 2528
TaqII	GACCGA	1 cut(s)	2447
TatI	WGTACW	2 cut(s)	2361, 2374
TauI	GCSGC	2 cut(s)	48, 2008
TfiI	GAWTC	8 cut(s)	90, 1310, 1384, 1442, 1487, 1499, 1769, 1937
TscAI	CASTG	4 cut(s)	70, 375, 886, 1000
TseFI	GTSAC	3 cut(s)	1027, 1278, 2119
Tsp45I	GTSAC	3 cut(s)	1027, 1278, 2119
TspGWI	ACGGA	3 cut(s)	818, 1511, 2438
TspMI	CCCGGG	1 cut(s)	128
TspRI	CASTG	4 cut(s)	70, 375, 886, 1000
Tth111I	GACNNNGTC	1 cut(s)	2554
Vha464I	CTTAAG	1 cut(s)	1924
VpaK11BI	GGWCC	1 cut(s)	805
VspI	ATTAAT	1 cut(s)	270
XapI	RAATTY	9 cut(s)	180, 688, 955, 1159, 1172, 1208, 1706, 2172, 2387
XbaI	TCTAGA	1 cut(s)	667
XceI	RCATGY	1 cut(s)	2024
XhoI	CTCGAG	2 cut(s)	313, 440
XmaI	CCCGGG	1 cut(s)	128
XmiI	GTMKAC	2 cut(s)	1098, 2060
XspI	CTAG	9 cut(s)	668, 1362, 1593, 1632, 1787, 2045, 2096, 2145, 2513

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.