Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr4C

Orthogroup ID

OG0008343

Physical Location & Seq

Genomic Coordinates Forward (+)

13297139 .. 13308235

Gene Structure 11097 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh4CG072000.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2586 bp

ATGGCGGCTTCTCTCCACACCCACCACTGCCATCTTCATCTCCGCCGCTCTACCCCCTCCAAGCTCCCCACCCATCAAGCCCCCATATTCTCACGGCGTTTCCCGACCGGAAAACAAAGGGCTCTTGCTCGTGGACAGAACAACCGCAAGGGTGGTAGAGGTGGAACAACTGACAGCTCGTTCTGCTGGGTGTGCAGAGCTGGTTTGGAGATTGATAAGGTCTCGCCTGATGATGAAGGAAGTGAAAGGCCTCCTTTTGATATCAATTTGGCCGTCATTCTCGCCGGTTTCGCTTTCGAGGCCTATTCTAGCCCACCTGCTAATGTTGGAAGGCGTGAAATTGATGCTGCAGATTGCAAGACAGTGTACCTTTCAGAGTCATTTGTGCGTGAGATATACGATGGCCAACTATCGGTGAAATTGAAAAAAGGCGTCAATCTTCCTGCCATGGATCCATGGGGAACAAGTGATCCATATGTGATCATGCAATTAGATGGTCAAGTTAATAAAAGCAAGATTAAATGGGGGACAAAAGAACCAACTTGGAACGAAGACTTCAATTTTAATATCAAGCAGCCTCCAACCAAAAATCTTCAGGTTGCTGCTTGGGATGCAAACCTTGTAACTCCACATAAGCGCATGGGGAATGCAGGCATCAATCTAGAATGCCTTTGTGATGGGAATTTGCATGAAATACTGGTGGAATTGGAAGGAATGGGAGGTGGTGGGAAATTACTTATAGAGGTCAGATATGAAACTTTTGAAGAAATTGATGAGGGGAAAAAGTGGTGGATGAGGGTCCCCATTGTTTCTGAATTTCTCCGTAATAAGGGTTTTGAACCTGCTCTGAAAAAGTTTGTTGGCTCTGACACTGTGCAAGCACGTCAGTTTGCAGAATATGCTTTTGGGCAGTTAAAGTCATTCAATAATAATGCTTACCTTTGGAAGAATCTGATTTCAAGTAATGATGAAAGTGATAGGAAGGGCCCTGGGAAACCTAATAATTCTGCTGTTGTGTCAGGCGTGCCTTCCCAGATGGAGGGTATCGCAGAGGGTTCTTTAGATAATGCAGACTGCAATGAAGTGAGCAATTTAGACGAGTCTAACAGAGATAATGGTGATGTGGAGAATGGAAATGCCTCTCAGCCAGTGAAAAAACTCGATGATGAAATGCAGTCAGATAAGAACTTTTGGAAGAACTTTGCTAATGAGATCAATCAAAATGTTGTAGAGAAATTTGGATTGCCAGTGCCAGGAAAATTAAAGTGGGATGGGTTTGATTTATTAGACAGAGTTGGTTTGCAGTCACAAAAGATAGCAGAAGCAACTTATGTTGAATCAGGGCTTGCAACTCCAGAAGTCTTAGATGTTGATAACGATAAAACAACAGCCCCACTTCCCATTAGCATTATGCAGTCTTCATTTCCAGATATAAAGGAAGCAACAAGGGAACTATTAAAGCAAACTGATTCTGTTTTAGGAATATTAATGGTTCTAACTGCAACAGTATCTCAAGCAAAGAAGGAAGAAAATATTGTGGGAGGGAGTGCAACTAAGGAAGAGGATGACGTCGTAACTGAGAAGCTTGCTAACTCACAGGGAGCCGAGGAGATGAAAGCACTGTTCTTAACTGCAGAAACTGCCATGGAGGCTTGGGCGATGCTCGCTACTTCTTTAGGACATCCAAGTTTTATAAAGTCTGAATTTGAGAAACTATGTTTCTTAGATAATGAGACAACAGACACACAGGTTGCAATTTGGCGTGATTCTGCACGGAAAAGATTAGTGATCGCTTTCAGGGGAACAGAGCAGGCAAGATGGAAGGACTTGCGAACGGATTTGATGCTAGCTCCTGCTGGGCTGAATCCAGAAAGGATAGGAGGAAACTTTAAGCAAGAAATTCAAGTTCATAGTGGCTTCTTAGGTGCATATGATTCTGTCAGAATAAGGATCATCTCTCTAATTAAATTAGCAATCGGTTATATTGATGATCTTGCTGAGCCGGTGCACAGATGGCATGTTTATGTAACTGGTCATAGTCTTGGTGGTGCATTGGCTACTCTCCTAGCTCTCGAACTTGCATCAAGTCAATTAGCAAAGCATGGAGTGATCACTATAAGCATGTATAATTTCGGATCTCCTAGGGTTGGGAACAAAAGATTTGCTGATGTTTATAATGAGAAAGTCAAAGATAGTTGGAGAGTTGTAAATCACAGAGACATTATACCAACTGTGCCCCGCTTGATGGGTTACTGCCATGTAGCTCAACCTGTTTATCTAGCAACAGGAGACCTAGCGAATGCCTTGGAAAATATGGAGCTCTCTGGAGATGGTTACCAAGCTGATATGATCGGGGAGTCCACACCGGATGTTCTCGTCAGTGAATTCATGAAGGGTGAGAAGGAACTTATACAGAAAATTTTACAGACTGAAATAAATATATTCCGTTCAATCAGAGATGGAACTGCACTTATGCAACATATGGAAGATTTTTACTACATCACGTTGCTTGAGAATGTGAGATCAAATTACCAACCTGTTGCCAAGTCGTTATCCGACGAACAATCCAACAAAACAATCAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001505 GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005783 GO:0005789 GO:0007154 GO:0007165 GO:0008021 GO:0008150 GO:0009987 GO:0010646 GO:0012505 GO:0012506 GO:0016020 GO:0016021 GO:0019722 GO:0019932 GO:0023051 GO:0023052 GO:0030100 GO:0030133 GO:0030334 GO:0030336 GO:0030658 GO:0030659 GO:0030672 GO:0031090 GO:0031224 GO:0031410 GO:0031982 GO:0031984 GO:0032879 GO:0035556 GO:0040012 GO:0040013 GO:0042175 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044433 GO:0044444 GO:0044446 GO:0044456 GO:0044464 GO:0045202 GO:0045806 GO:0046872 GO:0046928 GO:0048167 GO:0048168 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050804 GO:0050896 GO:0051046 GO:0051049 GO:0051051 GO:0051128 GO:0051129 GO:0051270 GO:0051271 GO:0051588 GO:0051716 GO:0055037 GO:0060341 GO:0060627 GO:0065007 GO:0065008 GO:0070382 GO:0080134 GO:0097458 GO:0097708 GO:0098588 GO:0098793 GO:0098805 GO:0098827 GO:0099177 GO:0099501 GO:0099503 GO:1902882 GO:1902883 GO:1903530 GO:2000145 GO:2000146

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

C2 Lipase_3

Protein Analysis

861

Amino Acids

95.68

Weight (kDa)

5.45

Isoelectric Point (pI)

40.4

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	135 - 230	1.3e-19	C2 domain
Lipase_3	PF01764	596 - 747	3e-28	Lipase (class 3)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008343)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13550 AT4G13550 AT4G13550
fragaria_vesca	FvH4_4g05250 FvH4_4g05250
malus_domestica	MD13G1226600.v1.1
prunus_persica	Prupe.1G054000_v2.0.a1 Prupe.1G054000_v2.0.a1 Prupe.1G054000_v2.0.a1 Prupe.1G054000_v2.0.a1 Prupe.1G054000_v2.0.a1
pyrus_communis	pycom13g19960
rosa_chinensis	RchiOBHm_Chr4g0396161
rosa_laevigata	RLG00000009564
rosa_multiflora	Rmu_sc0006325.1_g000041
rosa_roxburghii	Rroxscaffold_5G00341010
rosa_rugosa	Rorug03G0351200 Rorug03G0351300 Rorug03G0351300
rosa_samantha	Rh4BG065200 Rh4CG072000 Rh4DG062000
rosa_wichuraiana	Rw4G005390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1694, 2175
AarI	CACCTGC	1 cut(s)	325
AatII	GACGTC	1 cut(s)	1572
Acc36I	ACCTGC	2 cut(s)	325, 850
AccBSI	CCGCTC	1 cut(s)	48
AciI	CCGC	5 cut(s)	5, 43, 46, 145, 2239
AclWI	GGATC	5 cut(s)	446, 459, 464, 1958, 2143
AcoI	YGGCCR	2 cut(s)	270, 403
AcsI	RAATTY	7 cut(s)	682, 815, 1235, 1703, 1899, 2384, 2418
AcuI	CTGAAG	1 cut(s)	578
AcyI	GRCGYC	2 cut(s)	432, 1569
AfaI	GTAC	1 cut(s)	368
AfiI	CCNNNNNNNGG	6 cut(s)	412, 829, 1039, 1253, 2147, 2245
AgsI	TTSAA	9 cut(s)	424, 559, 764, 839, 925, 960, 1337, 1904, 2451
AjiI	CACGTC	1 cut(s)	884
AjnI	CCWGG	2 cut(s)	988, 1252
AjuI	GAANNNNNNNTTGG	2 cut(s)	888, 920
AloI	GAACNNNNNNTCC	2 cut(s)	454, 486
Alw21I	GWGCWC	2 cut(s)	2010, 2322
Alw26I	GTCTC	4 cut(s)	226, 1727, 2211, 2283
Alw44I	GTGCAC	1 cut(s)	2006
AlwI	GGATC	5 cut(s)	446, 459, 464, 1958, 2143
AlwNI	CAGNNNCTG	1 cut(s)	1640
AoxI	GGCC	5 cut(s)	248, 270, 300, 403, 985
ApaI	GGGCCC	1 cut(s)	989
ApaLI	GTGCAC	1 cut(s)	2006
ApeKI	GCWGC	3 cut(s)	347, 574, 602
ApoI	RAATTY	7 cut(s)	682, 815, 1235, 1703, 1899, 2384, 2418
AseI	ATTAAT	1 cut(s)	1487
AspA2I	CCTAGG	1 cut(s)	2141
AspLEI	GCGC	1 cut(s)	639
AspS9I	GGNCC	3 cut(s)	799, 985, 986
AsuHPI	GGTGA	3 cut(s)	427, 1130, 2408
AsuNHI	GCTAGC	1 cut(s)	1846
AvaII	GGWCC	1 cut(s)	799
AvrII	CCTAGG	1 cut(s)	2141
BaeGI	GKGCMC	3 cut(s)	989, 2010, 2238
BalI	TGGCCA	1 cut(s)	405
BamHI	GGATCC	1 cut(s)	451
BanII	GRGCYC	3 cut(s)	124, 989, 2322
BauI	CACGAG	1 cut(s)	129
BbsI	GAAGAC	2 cut(s)	558, 1410
Bbv12I	GWGCWC	2 cut(s)	2010, 2322
BbvI	GCAGC	3 cut(s)	334, 586, 589
BceAI	ACGGC	2 cut(s)	110, 257
BciT130I	CCWGG	2 cut(s)	990, 1254
BclI	TGATCA	2 cut(s)	480, 2109
BcoDI	GTCTC	4 cut(s)	226, 1727, 2211, 2283
BfaI	CTAG	7 cut(s)	309, 662, 1847, 2066, 2142, 2279, 2294
BfmI	CTRYAG	2 cut(s)	348, 1632
BfuAI	ACCTGC	2 cut(s)	325, 850
BglI	GCCNNNNNGGC	1 cut(s)	1649
BisI	GCNGC	5 cut(s)	6, 46, 348, 575, 603
BlnI	CCTAGG	1 cut(s)	2141
BlpI	GCTNAGC	1 cut(s)	1998
BlsI	GCNGC	5 cut(s)	7, 47, 349, 576, 604
Bme1390I	CCNGG	2 cut(s)	990, 1254
Bme18I	GGWCC	1 cut(s)	799
BmgBI	CACGTC	1 cut(s)	884
BmgT120I	GGNCC	3 cut(s)	799, 985, 986
BmiI	GGNNCC	5 cut(s)	453, 800, 801, 987, 1603
BmrFI	CCNGG	2 cut(s)	990, 1254
BmsI	GCATC	6 cut(s)	334, 601, 663, 1650, 1833, 2090
BmtI	GCTAGC	1 cut(s)	1850
BpiI	GAAGAC	2 cut(s)	558, 1410
BpmI	CTGGAG	2 cut(s)	1338, 2346
Bpu1102I	GCTNAGC	1 cut(s)	1998
BpuEI	CTTGAG	2 cut(s)	1497, 2531
BsaHI	GRCGYC	2 cut(s)	432, 1569
BsaI	GGTCTC	2 cut(s)	226, 2283
BsaJI	CCNNGG	8 cut(s)	447, 455, 988, 989, 1605, 1644, 2141, 2304
BsaWI	WCCGGW	2 cut(s)	107, 2365
Bsc4I	CCNNNNNNNGG	6 cut(s)	412, 829, 1039, 1253, 2147, 2245
Bse118I	RCCGGY	2 cut(s)	284, 2002
Bse1I	ACTGG	4 cut(s)	702, 1148, 1247, 2035
Bse3DI	GCAATG	1 cut(s)	1084
BseBI	CCWGG	2 cut(s)	990, 1254
BseDI	CCNNGG	8 cut(s)	447, 455, 988, 989, 1605, 1644, 2141, 2304
BseGI	GGATG	6 cut(s)	616, 798, 1276, 1570, 1681, 2374
BseLI	CCNNNNNNNGG	6 cut(s)	412, 829, 1039, 1253, 2147, 2245
BseMI	GCAATG	1 cut(s)	1084
BseMII	CTCAG	3 cut(s)	1157, 1569, 1989
BseNI	ACTGG	4 cut(s)	702, 1148, 1247, 2035
BseRI	GAGGAG	1 cut(s)	1622
BseSI	GKGCMC	3 cut(s)	989, 2010, 2238
BseXI	GCAGC	3 cut(s)	334, 586, 589
BseYI	CCCAGC	2 cut(s)	186, 1856
BsgI	GTGCAG	3 cut(s)	214, 1755, 2451
Bsh1285I	CGRYCG	1 cut(s)	108
BshFI	GGCC	5 cut(s)	250, 272, 302, 405, 987
BsiEI	CGRYCG	1 cut(s)	108
BsiHKAI	GWGCWC	2 cut(s)	2010, 2322
BsiSI	CCGG	4 cut(s)	108, 285, 2003, 2366
BslFI	GGGAC	2 cut(s)	541, 785
BslI	CCNNNNNNNGG	6 cut(s)	412, 829, 1039, 1253, 2147, 2245
BsmAI	GTCTC	4 cut(s)	226, 1727, 2211, 2283
BsmFI	GGGAC	2 cut(s)	541, 785
BsmI	GAATGC	3 cut(s)	652, 671, 2305
BsnI	GGCC	5 cut(s)	250, 272, 302, 405, 987
Bso31I	GGTCTC	2 cut(s)	226, 2283
Bsp120I	GGGCCC	1 cut(s)	985
Bsp1286I	GDGCHC	5 cut(s)	124, 989, 2010, 2238, 2322
Bsp1720I	GCTNAGC	1 cut(s)	1998
Bsp19I	CCATGG	3 cut(s)	447, 455, 1644
BspACI	CCGC	5 cut(s)	5, 43, 46, 145, 2239
BspANI	GGCC	5 cut(s)	250, 272, 302, 405, 987
BspCNI	CTCAG	3 cut(s)	1156, 1570, 1990
BspHI	TCATGA	1 cut(s)	2388
BspLI	GGNNCC	5 cut(s)	453, 800, 801, 987, 1603
BspMAI	CTGCAG	2 cut(s)	352, 1636
BspMI	ACCTGC	2 cut(s)	325, 850
BspOI	GCTAGC	1 cut(s)	1850
BspPI	GGATC	5 cut(s)	446, 459, 464, 1958, 2143
BspTNI	GGTCTC	2 cut(s)	226, 2283
BsrBI	CCGCTC	1 cut(s)	48
BsrDI	GCAATG	1 cut(s)	1084
BsrFI	RCCGGY	2 cut(s)	284, 2002
BsrI	ACTGG	4 cut(s)	702, 1148, 1247, 2035
BssAI	RCCGGY	2 cut(s)	284, 2002
BssECI	CCNNGG	8 cut(s)	447, 455, 988, 989, 1605, 1644, 2141, 2304
BssNI	GRCGYC	2 cut(s)	432, 1569
BssSI	CACGAG	1 cut(s)	129
BssT1I	CCWWGG	5 cut(s)	447, 455, 1644, 2141, 2304
Bst2BI	CACGAG	1 cut(s)	129
Bst2UI	CCWGG	2 cut(s)	990, 1254
Bst4CI	ACNGT	5 cut(s)	364, 874, 1507, 1623, 2233
Bst6I	CTCTTC	1 cut(s)	1554
BstACI	GRCGYC	2 cut(s)	432, 1569
BstAPI	GCANNNNNTGC	2 cut(s)	899, 1640
BstC8I	GCNNGC	8 cut(s)	652, 879, 1025, 1347, 1587, 1665, 1812, 1848
BstDEI	CTNAG	7 cut(s)	1143, 1363, 1554, 1578, 1723, 1921, 1998
BstDSI	CCRYGG	3 cut(s)	447, 455, 1644
BstEII	GGTNACC	1 cut(s)	2333
BstF5I	GGATG	6 cut(s)	616, 798, 1276, 1570, 1681, 2374
BstHHI	GCGC	1 cut(s)	639
BstMAI	GTCTC	4 cut(s)	226, 1727, 2211, 2283
BstMCI	CGRYCG	1 cut(s)	108
BstNI	CCWGG	2 cut(s)	990, 1254
BstNSI	RCATGY	2 cut(s)	2021, 2125
BstPI	GGTNACC	1 cut(s)	2333
BstSCI	CCNGG	2 cut(s)	988, 1252
BstSFI	CTRYAG	2 cut(s)	348, 1632
BstSLI	GKGCMC	3 cut(s)	989, 2010, 2238
BstV1I	GCAGC	3 cut(s)	334, 586, 589
BstV2I	GAAGAC	2 cut(s)	558, 1410
BstX2I	RGATCY	2 cut(s)	451, 2135
BstYI	RGATCY	2 cut(s)	451, 2135
BsuRI	GGCC	5 cut(s)	250, 272, 302, 405, 987
BtgI	CCRYGG	3 cut(s)	447, 455, 1644
BtgZI	GCGATG	1 cut(s)	1673
BtrI	CACGTC	1 cut(s)	884
BtsCI	GGATG	6 cut(s)	616, 798, 1276, 1570, 1681, 2374
BtsI	GCAGTG	1 cut(s)	25
BtsIMutI	CAGTG	7 cut(s)	25, 369, 870, 1155, 1254, 1619, 2386
BveI	ACCTGC	2 cut(s)	325, 850
Cac8I	GCNNGC	8 cut(s)	652, 879, 1025, 1347, 1587, 1665, 1812, 1848
CaiI	CAGNNNCTG	1 cut(s)	1640
CciI	TCATGA	1 cut(s)	2388
CfoI	GCGC	1 cut(s)	639
Cfr10I	RCCGGY	2 cut(s)	284, 2002
Cfr13I	GGNCC	3 cut(s)	799, 985, 986
CseI	GACGC	1 cut(s)	421
Csp6I	GTAC	1 cut(s)	367
CviQI	GTAC	1 cut(s)	367
DdeI	CTNAG	7 cut(s)	1143, 1363, 1554, 1578, 1723, 1921, 1998
EaeI	YGGCCR	2 cut(s)	270, 403
Eam1104I	CTCTTC	1 cut(s)	1554
EarI	CTCTTC	1 cut(s)	1554
EciI	GGCGGA	1 cut(s)	32
Ecl136II	GAGCTC	1 cut(s)	2320
Eco130I	CCWWGG	5 cut(s)	447, 455, 1644, 2141, 2304
Eco147I	AGGCCT	2 cut(s)	250, 302
Eco24I	GRGCYC	3 cut(s)	124, 989, 2322
Eco31I	GGTCTC	2 cut(s)	226, 2283
Eco32I	GATATC	1 cut(s)	262
Eco47I	GGWCC	1 cut(s)	799
Eco53kI	GAGCTC	1 cut(s)	2320
Eco57I	CTGAAG	1 cut(s)	578
Eco91I	GGTNACC	1 cut(s)	2333
EcoICRI	GAGCTC	1 cut(s)	2320
EcoO109I	RGGNCCY	3 cut(s)	799, 985, 986
EcoO65I	GGTNACC	1 cut(s)	2333
EcoRI	GAATTC	1 cut(s)	2384
EcoRII	CCWGG	2 cut(s)	988, 1252
EcoRV	GATATC	1 cut(s)	262
EcoT14I	CCWWGG	5 cut(s)	447, 455, 1644, 2141, 2304
EcoT38I	GRGCYC	3 cut(s)	124, 989, 2322
ErhI	CCWWGG	5 cut(s)	447, 455, 1644, 2141, 2304
FalI	AAGNNNNNCTT	2 cut(s)	238, 270
FaqI	GGGAC	2 cut(s)	541, 785
FauI	CCCGC	1 cut(s)	2246
FauNDI	CATATG	3 cut(s)	475, 1930, 2481
FbaI	TGATCA	2 cut(s)	480, 2109
Fnu4HI	GCNGC	5 cut(s)	6, 46, 348, 575, 603
FokI	GGATG	6 cut(s)	623, 805, 1283, 1577, 1668, 2381
FriOI	GRGCYC	3 cut(s)	124, 989, 2322
Fsp4HI	GCNGC	5 cut(s)	6, 46, 348, 575, 603
FspBI	CTAG	7 cut(s)	309, 662, 1847, 2066, 2142, 2279, 2294
GlaI	GCGC	1 cut(s)	638
GluI	GCNGC	5 cut(s)	6, 46, 348, 575, 603
GsaI	CCCAGC	2 cut(s)	190, 1860
GsuI	CTGGAG	2 cut(s)	1338, 2346
HaeIII	GGCC	5 cut(s)	250, 272, 302, 405, 987
HapII	CCGG	4 cut(s)	108, 285, 2003, 2366
HgaI	GACGC	1 cut(s)	421
HhaI	GCGC	1 cut(s)	639
Hin1I	GRCGYC	2 cut(s)	432, 1569
Hin6I	GCGC	1 cut(s)	637
HinP1I	GCGC	1 cut(s)	637
HindIII	AAGCTT	1 cut(s)	1583
HinfI	GANTC	9 cut(s)	377, 949, 1100, 1337, 1469, 1766, 1864, 1934, 2357
HpaII	CCGG	4 cut(s)	108, 285, 2003, 2366
HphI	GGTGA	3 cut(s)	427, 1130, 2408
Hpy166II	GTNNAC	4 cut(s)	134, 367, 2008, 2361
Hpy188III	TCNNGA	8 cut(s)	103, 662, 1355, 1427, 1868, 2072, 2325, 2389
Hpy8I	GTNNAC	4 cut(s)	134, 367, 2008, 2361
Hpy99I	CGWCG	2 cut(s)	1574, 2561
HpyAV	CCTTC	9 cut(s)	230, 324, 704, 976, 1038, 1516, 1816, 2386, 2395
HpyCH4III	ACNGT	5 cut(s)	364, 874, 1507, 1623, 2233
HpyCH4IV	ACGT	3 cut(s)	883, 1569, 2504
HpyF3I	CTNAG	7 cut(s)	1143, 1363, 1554, 1578, 1723, 1921, 1998
HpySE526I	ACGT	3 cut(s)	883, 1569, 2504
Hsp92I	GRCGYC	2 cut(s)	432, 1569
HspAI	GCGC	1 cut(s)	637
KflI	GGGWCCC	1 cut(s)	799
Ksp22I	TGATCA	2 cut(s)	480, 2109
LmnI	GCTCC	4 cut(s)	69, 1601, 1855, 2317
Lsp1109I	GCAGC	3 cut(s)	334, 586, 589
LweI	GCATC	6 cut(s)	334, 601, 663, 1650, 1833, 2090
MaeI	CTAG	7 cut(s)	309, 662, 1847, 2066, 2142, 2279, 2294
MaeII	ACGT	3 cut(s)	883, 1569, 2504
MaeIII	GTNAC	6 cut(s)	622, 1305, 1573, 2026, 2249, 2333
MbiI	CCGCTC	1 cut(s)	48
MflI	RGATCY	2 cut(s)	451, 2135
MhlI	GDGCHC	5 cut(s)	124, 989, 2010, 2238, 2322
MlsI	TGGCCA	1 cut(s)	405
MluNI	TGGCCA	1 cut(s)	405
MlyI	GAGTC	3 cut(s)	386, 1109, 2366
MmeI	TCCRAC	4 cut(s)	307, 605, 2177, 2580
Mox20I	TGGCCA	1 cut(s)	405
MscI	TGGCCA	1 cut(s)	405
MslI	CAYNNNNRTG	1 cut(s)	2022
Msp20I	TGGCCA	1 cut(s)	405
MspA1I	CMGCKG	1 cut(s)	2583
MspI	CCGG	4 cut(s)	108, 285, 2003, 2366
MspR9I	CCNGG	2 cut(s)	990, 1254
Mva1269I	GAATGC	3 cut(s)	652, 671, 2305
MvaI	CCWGG	2 cut(s)	990, 1254
NcoI	CCATGG	3 cut(s)	447, 455, 1644
NdeI	CATATG	3 cut(s)	475, 1930, 2481
NheI	GCTAGC	1 cut(s)	1846
NlaIV	GGNNCC	5 cut(s)	453, 800, 801, 987, 1603
NmeAIII	GCCGAG	1 cut(s)	1630
NmuCI	GTSAC	1 cut(s)	1305
NspI	RCATGY	2 cut(s)	2021, 2125
PagI	TCATGA	1 cut(s)	2388
PaqCI	CACCTGC	1 cut(s)	325
PasI	CCCWGGG	1 cut(s)	989
PceI	AGGCCT	2 cut(s)	250, 302
PctI	GAATGC	3 cut(s)	652, 671, 2305
PfeI	GAWTC	6 cut(s)	949, 1337, 1469, 1766, 1864, 1934
PkrI	GCNGC	5 cut(s)	7, 47, 349, 576, 604
PleI	GAGTC	3 cut(s)	385, 1108, 2365
PpsI	GAGTC	3 cut(s)	385, 1108, 2365
PpuMI	RGGWCCY	1 cut(s)	799
PshBI	ATTAAT	1 cut(s)	1487
PsiI	TTATAA	2 cut(s)	1694, 2175
Psp124BI	GAGCTC	1 cut(s)	2322
Psp5II	RGGWCCY	1 cut(s)	799
Psp6I	CCWGG	2 cut(s)	988, 1252
PspEI	GGTNACC	1 cut(s)	2333
PspFI	CCCAGC	2 cut(s)	186, 1856
PspGI	CCWGG	2 cut(s)	988, 1252
PspN4I	GGNNCC	5 cut(s)	453, 800, 801, 987, 1603
PspOMI	GGGCCC	1 cut(s)	985
PspPI	GGNCC	3 cut(s)	799, 985, 986
PspPPI	RGGWCCY	1 cut(s)	799
PstI	CTGCAG	2 cut(s)	352, 1636
PstNI	CAGNNNCTG	1 cut(s)	1640
PsuI	RGATCY	2 cut(s)	451, 2135
PvuII	CAGCTG	1 cut(s)	2583
RsaI	GTAC	1 cut(s)	368
RsaNI	GTAC	1 cut(s)	367
RseI	CAYNNNNRTG	1 cut(s)	2022
SacI	GAGCTC	1 cut(s)	2322
SatI	GCNGC	5 cut(s)	6, 46, 348, 575, 603
Sau96I	GGNCC	3 cut(s)	799, 985, 986
SchI	GAGTC	3 cut(s)	386, 1109, 2366
ScrFI	CCNGG	2 cut(s)	990, 1254
SduI	GDGCHC	5 cut(s)	124, 989, 2010, 2238, 2322
SfaNI	GCATC	6 cut(s)	334, 601, 663, 1650, 1833, 2090
SfcI	CTRYAG	2 cut(s)	348, 1632
SinI	GGWCC	1 cut(s)	799
SmiMI	CAYNNNNRTG	1 cut(s)	2022
SmlI	CTYRAG	2 cut(s)	1512, 2510
SmoI	CTYRAG	2 cut(s)	1512, 2510
SseBI	AGGCCT	2 cut(s)	250, 302
SsiI	CCGC	5 cut(s)	5, 43, 46, 145, 2239
SspI	AATATT	2 cut(s)	1485, 1534
SspMI	CTAG	7 cut(s)	309, 662, 1847, 2066, 2142, 2279, 2294
SstI	GAGCTC	1 cut(s)	2322
StuI	AGGCCT	2 cut(s)	250, 302
StyD4I	CCNGG	2 cut(s)	988, 1252
StyI	CCWWGG	5 cut(s)	447, 455, 1644, 2141, 2304
TaaI	ACNGT	5 cut(s)	364, 874, 1507, 1623, 2233
TaiI	ACGT	3 cut(s)	886, 1572, 2507
TaqI	TCGA	3 cut(s)	297, 1161, 2073
TauI	GCSGC	2 cut(s)	8, 48
TfiI	GAWTC	6 cut(s)	949, 1337, 1469, 1766, 1864, 1934
TscAI	CASTG	7 cut(s)	32, 369, 877, 1155, 1254, 1626, 2386
TseFI	GTSAC	1 cut(s)	1305
TseI	GCWGC	3 cut(s)	347, 574, 602
Tsp45I	GTSAC	1 cut(s)	1305
TspGWI	ACGGA	4 cut(s)	812, 1789, 1850, 2435
TspRI	CASTG	7 cut(s)	32, 369, 877, 1155, 1254, 1626, 2386
VneI	GTGCAC	1 cut(s)	2006
VpaK11BI	GGWCC	1 cut(s)	799
VspI	ATTAAT	1 cut(s)	1487
XapI	RAATTY	7 cut(s)	682, 815, 1235, 1703, 1899, 2384, 2418
XbaI	TCTAGA	1 cut(s)	661
XceI	RCATGY	2 cut(s)	2021, 2125
XmaJI	CCTAGG	1 cut(s)	2141
XspI	CTAG	7 cut(s)	309, 662, 1847, 2066, 2142, 2279, 2294
ZraI	GACGTC	1 cut(s)	1570

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.