Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000003

Orthogroup ID

OG0008343

Physical Location & Seq

Genomic Coordinates Forward (+)

42955920 .. 42965975

Gene Structure 10056 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug03G0351300.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3348 bp

ATGGCCGCCGATCCAAGTCAGCAGCAGCAGCTGGCCATAATTCTTGGCCCGGACCCGAGTCAGTTCGAAACCCTAATCTCTCATCTCATGTCCTCCAGCAACGAGCAACGGTCGCAGGCCGAGGCCCTCTTCAACCTCTGCAAGCAGGCCCACCCGGACGCCCTCTTACTGAAGCTGGTCCATGTGTTACAGGCCTCGACCCGACCCGAGGCCCGCACCATGTCCGTCATCCTCCTCCGGCGGCAGCTCACTCGCGACGACTCGTTCCTCTGGCCCCGCCTCAGGCCCTCCACTCAGTCGACCCTCAAGTCCACACTCCTCACTTGCCTCCAATCAGAATCCACCAAATCCATGGCCAAGAAGCTCTGCGACACCGTTTCGGAGCTCGCTTCCGCCATCTTACCCGATAACCAATGGCCGGAGCTGTTGCCGTTCATGTTCCACTGCGTCACCTCCGATAATTCCAAGCTCCAGGAGTCTGCGCTTTTGATTTTTGCGCAGTTGGCGCATTACATCGGCGAAACCCTAGTCCCGCAGTTGACTACGCTCCACTCGGTCTTCTACCAGTGCCTGTCCAACGCCGCCGGCAAGAGCGCCGACGTCAGAATTGCAGCACTCGGCGCCTCTGTCAATTTCATCCAGTGCCTGACCAGCGCCTCCGACAGGGACAGGTTCCAGGACCTGTTGCCCTTGATGATGCAGACTTTGACGGAGGCCTTGAACGGCGGTCAGGAGGCCACGGCGCAGGAGGCGCTGGAGTTGCTTATCGAGTTGGCCGGGACCGAGCCGAGGTTCCTGCGGAGGCAATTGGTGGATGTGGTTGGCTCTATGCTTCAAATTGCGGAGGCTGCGTCCCTCGAGGAAGGTACAAGGCATTTGGCCATTGAGTTTGTCATCACTCTGGCTGAGGCCAGGGAGAGAGCCCCCGGGATGATGAGGAAGTTGCCTCAGTTTATTCATAGGTTGTTTGCCATACTTATGAACATGTTGTTGGACCTTGAGGACGACCCCGACTGGTACACTGCAGATACTGAGAATGAGGATGCTGGTGAGACTAGCAATTACGGCTTTGGACAGGAGTGTTTGGACAGACTTTCAATTTCATTGGGCGGCAATACCATTGTCCCTGTTGCTTCCGAGCTTTTTCCGTCTTACTTGGTTGCCCCTGAATGGCAAAAGCACCACGCCGTGCACATTGCCCTTGCCCAGATTGCCGAGGGGTGCTCTAAGGTAATGGTACAAAACCTCGAACAGGTGGTATCAATGGTATTGAATTCGTTCCAAGATCCTCACCCACGTGTGAGGTGGGCAGCAATTAATGCAGTTGGGCAATTGTCAACGGATTTAGGCCCAAACTTACAAGAAAAGTATCACCAGCGAGTGCTGCCTGCATTAGCTGGAGCAATGGATGACTTCCAAAATCCACGAGTACAGGCCCATGCTGCTTCAGCAGTACTCAATTTCAGTGAAAACTGCACCCCAGATATTTTGGTACCATACCTGGATGGAATTGTTAGCAAGCTTCTTGTACTTCTACAGAATGGAAAGCAGATGGTCCAGGAGGGGGCTTTAACAGCTTTAGCATCAGTTGCTGATTCGTCACAGGAGCAATTCCAGAAGTATTATGATGCTGTAATGCCTTACTTGAAAGCTATTTTGGTTAATGCAAATGACAAAGCTAATCGTATGCTACGTGCAAAATCAATGGAGTGTATTAGTTTGGTTGGAATGGCTGTCGGGAAGGATAAGTTCAGGGATGACGCAAAGCAGGTTATGGAAGTCCTGATGTCATTGCAAGGATCTGAAATGGAGGCAGATGACCCCACAACAAGTTATATGTTACAAGCATGGGCTAGACTTTGCAAATGCCTTGGGCAGGATTTTCTCCCGTACATGAATGTTGTCATGCCTTCTTTGCTTCAGTCTGCTCAGCTTAAGCCTGATGTAACAATTACTTCTGCAGATTCAGATGCAGAAATTGATGAAGATGATGATAGCATTGAAATGATTACTGTTGGGGATAAAAGGATAGGGATTAAGACTAGTGTTCTGGAGGAAAAAGCTACAGCCTGTAACATGCTATGTTGCTATGCTGATGAGTTAAAGGAAGGATTTTTCCCATGGATTGATCAGGTTGCTCCAACATTGGTTCCTCTTCTCAAATTTTATTTCCATGAAGAAGTTAGAAAGGCAGCTGTTTCAGCGATGCCGGAGCTCCTACGCTCAGCCAAATTAGCAGTAGAGAAGGGACAATCTCAAGGTCATAATGAGTCCTACGTAAAGCAGTTATCTGATTACATAATACCAGCACTGGTTGAAGCATTACACAAGGAACCTGAGGTAGAAATTTGTGCCAGCATTCTAGATGCATTGAACGAATGTGTGCAGAGTTCTGGGCCACTTCTTGATGAAAACCAAGTTAGATGCATCGTGGATGAGATCAAACAGGTGGTTACAGCAAGCTCATCCAGAAAACAAGAGCGAGCAGAAAGGGCCAAGGCAGAGGACTTTGATGCAGAAGAAGGGGAACTTCTGAAGGAGGAAAATGAACAAGAAGAAGAGCTTTTTGACCTGGTTGGTGATTGCTTGGGAACTTTGATTAAGACATTCAAGGCATCTTTCTTGCCTTTCTTTGACGAGCTTTCCTCTTACATAACACCTATGTTGGGTAAGGATAAAACTGCAGAAGAGAGGAGAATTGCCATTTGCATATTTGATGATATTGCAGAGCATTGCCGTGAAGCTGCTCTTAAATATTATGATACTTACGTTCCTTTCCTACTGGAAGCCTGCAATGATGAAAGTTCAGATGTTCGTCAGGCTGCTGTTTATGGGGTTGGTCTTTGTGCGGAGTATGGTGGATCTGTGTTCAAACCTCTTGTTGGTGAAGCTCTATCTAGGTTGGATATGGTGATCAGGAACCCCAATTCACAACACTCTGACAATATGATGGCATATGACAACGCTGTTTCAGCTCTTGGGAAAATATGCCAGTTCCACCGTGATAGTATAGATGCACCACAGCTTGTCCCTGCCTGGTTAGGTTGTCTGCCTATAAAAGGTGATTTGATTGAGGCCAAGGTTGTCCATGACCAGTTATGTTCAATGGTTGAAAGGTCGGATAGAGAGCTTTTGGGCCCCAATAATCAGTATCTTCCGAAAATAGTTGCGGTTTTTGCCGAGGTTATATGTGCGGGTAAAGACTTGGCCACAGAGCAAACAACTAGTCGAATGATTAATTTATTAAGGCAGCTTCAACAAACTTTACCGCCCTCTACCCTTGCGTCAACCTGGTCATCCTTGCAACCCCAGCAACAGCTTGCATTGCAATTTATACTCTCATCATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001505 GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005783 GO:0005789 GO:0007154 GO:0007165 GO:0008021 GO:0008150 GO:0009987 GO:0010646 GO:0012505 GO:0012506 GO:0016020 GO:0016021 GO:0019722 GO:0019932 GO:0023051 GO:0023052 GO:0030100 GO:0030133 GO:0030334 GO:0030336 GO:0030658 GO:0030659 GO:0030672 GO:0031090 GO:0031224 GO:0031410 GO:0031982 GO:0031984 GO:0032879 GO:0035556 GO:0040012 GO:0040013 GO:0042175 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044433 GO:0044444 GO:0044446 GO:0044456 GO:0044464 GO:0045202 GO:0045806 GO:0046872 GO:0046928 GO:0048167 GO:0048168 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050804 GO:0050896 GO:0051046 GO:0051049 GO:0051051 GO:0051128 GO:0051129 GO:0051270 GO:0051271 GO:0051588 GO:0051716 GO:0055037 GO:0060341 GO:0060627 GO:0065007 GO:0065008 GO:0070382 GO:0080134 GO:0097458 GO:0097708 GO:0098588 GO:0098793 GO:0098805 GO:0098827 GO:0099177 GO:0099501 GO:0099503 GO:1902882 GO:1902883 GO:1903530 GO:2000145 GO:2000146

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

C2 Lipase_3

Protein Analysis

1115

Amino Acids

123.54

Weight (kDa)

4.77

Isoelectric Point (pI)

48.13

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF577	PF04510	101 - 253	6.6e-07	Repeat of unknown function (DUF577)
TPR_IPO5	PF25780	115 - 274	2.2e-36	IPO5-like, TPR repeats
Importin_rep_4	PF18808	285 - 376	7.2e-23	Importin repeat
HEAT_EZ	PF13513	392 - 445	4.5e-06	HEAT-like repeat
TOR1L1_N	PF24714	392 - 578	2.3e-09	TORTIFOLIA1 N-terminal
HEAT_GCN1	PF23271	400 - 555	4.7e-06	Stalled ribosome sensor GCN1-like, HEAT repeats
HEAT_EZ	PF13513	475 - 529	3.8e-07	HEAT-like repeat
TPR_IMB1	PF25574	478 - 648	1e-11	Importin subunit beta-1-like, TPR repeats
Importin_rep_6	PF18829	784 - 890	1.9e-18	Importin repeat 6

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008343)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13550 AT4G13550 AT4G13550
fragaria_vesca	FvH4_4g05250 FvH4_4g05250
malus_domestica	MD13G1226600.v1.1
prunus_persica	Prupe.1G054000_v2.0.a1 Prupe.1G054000_v2.0.a1 Prupe.1G054000_v2.0.a1 Prupe.1G054000_v2.0.a1 Prupe.1G054000_v2.0.a1
pyrus_communis	pycom13g19960
rosa_chinensis	RchiOBHm_Chr4g0396161
rosa_laevigata	RLG00000009564
rosa_multiflora	Rmu_sc0006325.1_g000041
rosa_roxburghii	Rroxscaffold_5G00341010
rosa_rugosa	Rorug03G0351200 Rorug03G0351300 Rorug03G0351300
rosa_samantha	Rh4BG065200 Rh4CG072000 Rh4DG062000
rosa_wichuraiana	Rw4G005390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	307
AatII	GACGTC	1 cut(s)	603
AbsI	CCTCGAGG	1 cut(s)	857
Acc16I	TGCGCA	1 cut(s)	498
Acc36I	ACCTGC	1 cut(s)	1759
Acc65I	GGTACC	1 cut(s)	1492
AccB1I	GGYRCC	2 cut(s)	620, 1492
AccI	GTMKAC	1 cut(s)	299
AccII	CGCG	1 cut(s)	255
AclWI	GGATC	4 cut(s)	5, 1280, 1807, 2869
AcoI	YGGCCR	7 cut(s)	3, 33, 354, 416, 774, 879, 3207
AcsI	RAATTY	3 cut(s)	1273, 2162, 2346
AcuI	CTGAAG	4 cut(s)	191, 1431, 1904, 2555
AcvI	CACGTG	1 cut(s)	1298
AcyI	GRCGYC	3 cut(s)	159, 600, 621
AdeI	CACNNNGTG	1 cut(s)	1189
AfaI	GTAC	8 cut(s)	868, 1019, 1239, 1431, 1455, 1494, 1530, 1892
AflII	CTTAAG	1 cut(s)	1934
AflIII	ACRYGT	2 cut(s)	984, 1297
AhlI	ACTAGT	2 cut(s)	2042, 3224
AjnI	CCWGG	8 cut(s)	471, 675, 911, 1500, 1557, 2571, 3035, 3290
AjuI	GAANNNNNNNTTGG	4 cut(s)	974, 1006, 1640, 1672
AleI	CACNNNNGTG	1 cut(s)	1296
Alw21I	GWGCWC	4 cut(s)	387, 1194, 1226, 2217
Alw26I	GTCTC	1 cut(s)	1046
Alw44I	GTGCAC	1 cut(s)	1190
AlwI	GGATC	4 cut(s)	5, 1280, 1807, 2869
AlwNI	CAGNNNCTG	6 cut(s)	31, 571, 646, 682, 1031, 1592
Ama87I	CYCGRG	4 cut(s)	55, 206, 857, 926
ApaI	GGGCCC	1 cut(s)	3140
ApaLI	GTGCAC	1 cut(s)	1190
ApoI	RAATTY	3 cut(s)	1273, 2162, 2346
ArsI	GACNNNNNNTTYG	4 cut(s)	513, 545, 3211, 3243
AseI	ATTAAT	2 cut(s)	1317, 3237
Asp700I	GAANNNNTTC	1 cut(s)	1277
Asp718I	GGTACC	1 cut(s)	1492
AspLEI	GCGC	8 cut(s)	484, 499, 508, 596, 623, 656, 745, 754
AsuC2I	CCSGG	5 cut(s)	50, 155, 778, 927, 928
AsuHPI	GGTGA	8 cut(s)	442, 1061, 1283, 1364, 2591, 2897, 2923, 3074
AsuII	TTCGAA	1 cut(s)	66
AvaI	CYCGRG	4 cut(s)	55, 206, 857, 926
AvaII	GGWCC	6 cut(s)	52, 178, 679, 780, 994, 1555
AxyI	CCTNAGG	2 cut(s)	281, 2337
BaeGI	GKGCMC	2 cut(s)	1194, 3140
BaeI	ACNNNNGTAYC	2 cut(s)	2754, 2787
BalI	TGGCCA	4 cut(s)	35, 356, 881, 3209
BanI	GGYRCC	2 cut(s)	620, 1492
BanII	GRGCYC	4 cut(s)	387, 925, 2217, 3140
BauI	CACGAG	1 cut(s)	1425
BbrPI	CACGTG	1 cut(s)	1298
BbsI	GAAGAC	1 cut(s)	550
Bbv12I	GWGCWC	4 cut(s)	387, 1194, 1226, 2217
BbvCI	CCTCAGC	1 cut(s)	906
BccI	CCATC	4 cut(s)	404, 1499, 1546, 2944
BceAI	ACGGC	6 cut(s)	415, 739, 756, 1081, 1172, 2721
BcgI	CGANNNNNNTGC	4 cut(s)	233, 267, 778, 812
BciT130I	CCWGG	8 cut(s)	473, 677, 913, 1502, 1559, 2573, 3037, 3292
BclI	TGATCA	2 cut(s)	2128, 2913
BcnI	CCSGG	5 cut(s)	50, 155, 778, 927, 928
BcoDI	GTCTC	1 cut(s)	1046
BcuI	ACTAGT	2 cut(s)	2042, 3224
BfaI	CTAG	7 cut(s)	527, 1056, 1854, 2043, 2363, 2898, 3225
BfmI	CTRYAG	5 cut(s)	1023, 1535, 1959, 2064, 2682
BfoI	RGCGCY	4 cut(s)	597, 624, 657, 755
BfrI	CTTAAG	1 cut(s)	1934
BfuAI	ACCTGC	1 cut(s)	1759
BlpI	GCTNAGC	2 cut(s)	1929, 2224
BmcAI	AGTACT	1 cut(s)	1455
Bme18I	GGWCC	6 cut(s)	52, 178, 679, 780, 994, 1555
BmeT110I	CYCGRG	4 cut(s)	55, 206, 857, 926
BoxI	GACNNNNGTC	1 cut(s)	57
BpiI	GAAGAC	1 cut(s)	550
BplI	GAGNNNNNCTC	4 cut(s)	1208, 1240, 2630, 2662
BpmI	CTGGAG	5 cut(s)	79, 455, 776, 1419, 2072
Bpu10I	CCTNAGC	1 cut(s)	906
Bpu1102I	GCTNAGC	2 cut(s)	1929, 2224
Bpu14I	TTCGAA	1 cut(s)	66
BpuEI	CTTGAG	3 cut(s)	290, 1019, 2241
BpuMI	CCSGG	5 cut(s)	50, 155, 778, 927, 928
BsaAI	YACGTR	3 cut(s)	1298, 1694, 2278
BsaHI	GRCGYC	3 cut(s)	159, 600, 621
BsaXI	ACNNNNNCTCC	2 cut(s)	836, 866
Bse118I	RCCGGY	1 cut(s)	584
Bse1I	ACTGG	7 cut(s)	565, 640, 1019, 2316, 2787, 2992, 3094
Bse21I	CCTNAGG	2 cut(s)	281, 2337
Bse3DI	GCAATG	6 cut(s)	1194, 1410, 1790, 2731, 2800, 3323
BseBI	CCWGG	8 cut(s)	473, 677, 913, 1502, 1559, 2573, 3037, 3292
BseMI	GCAATG	6 cut(s)	1194, 1410, 1790, 2731, 2800, 3323
BseMII	CTCAG	8 cut(s)	295, 308, 897, 962, 1023, 1943, 2238, 2328
BseNI	ACTGG	7 cut(s)	565, 640, 1019, 2316, 2787, 2992, 3094
BseRI	GAGGAG	3 cut(s)	224, 308, 2707
BseSI	GKGCMC	2 cut(s)	1194, 3140
BseYI	CCCAGC	1 cut(s)	3309
BsgI	GTGCAG	2 cut(s)	1459, 2405
Bsh1236I	CGCG	1 cut(s)	255
Bsh1285I	CGRYCG	1 cut(s)	113
BshNI	GGYRCC	2 cut(s)	620, 1492
BsiEI	CGRYCG	1 cut(s)	113
BsiHKAI	GWGCWC	4 cut(s)	387, 1194, 1226, 2217
BsiHKCI	CYCGRG	4 cut(s)	55, 206, 857, 926
BsiSI	CCGG	8 cut(s)	50, 155, 238, 419, 585, 777, 927, 2210
BslFI	GGGAC	7 cut(s)	515, 680, 793, 838, 1109, 2262, 3014
BsmAI	GTCTC	1 cut(s)	1046
BsmFI	GGGAC	7 cut(s)	515, 680, 793, 838, 1109, 2262, 3014
BsmI	GAATGC	1 cut(s)	2358
BsoBI	CYCGRG	4 cut(s)	55, 206, 857, 926
Bsp119I	TTCGAA	1 cut(s)	66
Bsp120I	GGGCCC	1 cut(s)	3136
Bsp1286I	GDGCHC	6 cut(s)	387, 925, 1194, 1226, 2217, 3140
Bsp143I	GATC	7 cut(s)	10, 1285, 1799, 2128, 2439, 2861, 2913
Bsp1720I	GCTNAGC	2 cut(s)	1929, 2224
Bsp19I	CCATGG	2 cut(s)	351, 2120
Bsp68I	TCGCGA	1 cut(s)	255
BspCNI	CTCAG	8 cut(s)	294, 307, 898, 961, 1024, 1942, 2237, 2329
BspFNI	CGCG	1 cut(s)	255
BspMAI	CTGCAG	3 cut(s)	1027, 1963, 2686
BspMI	ACCTGC	1 cut(s)	1759
BspPI	GGATC	4 cut(s)	5, 1280, 1807, 2869
BspQI	GCTCTTC	1 cut(s)	2553
BspT104I	TTCGAA	1 cut(s)	66
BspT107I	GGYRCC	2 cut(s)	620, 1492
BspTI	CTTAAG	1 cut(s)	1934
BsrDI	GCAATG	6 cut(s)	1194, 1410, 1790, 2731, 2800, 3323
BsrFI	RCCGGY	1 cut(s)	584
BsrI	ACTGG	7 cut(s)	565, 640, 1019, 2316, 2787, 2992, 3094
BssAI	RCCGGY	1 cut(s)	584
BssMI	GATC	7 cut(s)	10, 1285, 1799, 2128, 2439, 2861, 2913
BssNI	GRCGYC	3 cut(s)	159, 600, 621
BssSI	CACGAG	1 cut(s)	1425
BssT1I	CCWWGG	5 cut(s)	351, 1870, 2120, 2496, 3078
Bst2BI	CACGAG	1 cut(s)	1425
Bst2UI	CCWGG	8 cut(s)	473, 677, 913, 1502, 1559, 2573, 3037, 3292
Bst4CI	ACNGT	4 cut(s)	111, 376, 2014, 3002
Bst6I	CTCTTC	4 cut(s)	134, 2160, 2553, 2682
BstACI	GRCGYC	3 cut(s)	159, 600, 621
BstAFI	CTTAAG	1 cut(s)	1934
BstAPI	GCANNNNNTGC	2 cut(s)	1319, 1589
BstBAI	YACGTR	3 cut(s)	1298, 1694, 2278
BstBI	TTCGAA	1 cut(s)	66
BstDEI	CTNAG	9 cut(s)	281, 294, 906, 948, 1032, 1227, 1929, 2224, 2337
BstDSI	CCRYGG	3 cut(s)	351, 738, 2120
BstENI	CCTNNNNNAGG	4 cut(s)	661, 1250, 1874, 3057
BstFNI	CGCG	1 cut(s)	255
BstH2I	RGCGCY	4 cut(s)	597, 624, 657, 755
BstHHI	GCGC	8 cut(s)	484, 499, 508, 596, 623, 656, 745, 754
BstKTI	GATC	7 cut(s)	13, 1288, 1802, 2131, 2442, 2864, 2916
BstMAI	GTCTC	1 cut(s)	1046
BstMBI	GATC	7 cut(s)	10, 1285, 1799, 2128, 2439, 2861, 2913
BstMCI	CGRYCG	1 cut(s)	113
BstNI	CCWGG	8 cut(s)	473, 677, 913, 1502, 1559, 2573, 3037, 3292
BstNSI	RCATGY	2 cut(s)	988, 2080
BstPAI	GACNNNNGTC	1 cut(s)	57
BstSFI	CTRYAG	5 cut(s)	1023, 1535, 1959, 2064, 2682
BstSLI	GKGCMC	2 cut(s)	1194, 3140
BstSNI	TACGTA	1 cut(s)	2278
BstUI	CGCG	1 cut(s)	255
BstV2I	GAAGAC	1 cut(s)	550
BstX2I	RGATCY	3 cut(s)	1285, 1799, 2861
BstXI	CCANNNNNNTGG	1 cut(s)	352
BstYI	RGATCY	3 cut(s)	1285, 1799, 2861
Bsu36I	CCTNAGG	2 cut(s)	281, 2337
BtgI	CCRYGG	3 cut(s)	351, 738, 2120
BtgZI	GCGATG	1 cut(s)	2219
BtsI	GCAGTG	2 cut(s)	442, 1020
BtsIMutI	CAGTG	6 cut(s)	442, 572, 647, 1020, 1471, 2309
BtuMI	TCGCGA	1 cut(s)	255
BveI	ACCTGC	1 cut(s)	1759
CaiI	CAGNNNCTG	6 cut(s)	31, 571, 646, 682, 1031, 1592
CfoI	GCGC	8 cut(s)	484, 499, 508, 596, 623, 656, 745, 754
Cfr10I	RCCGGY	1 cut(s)	584
Cfr9I	CCCGGG	1 cut(s)	926
CseI	GACGC	5 cut(s)	167, 436, 840, 1769, 3273
CsiI	ACCWGGT	2 cut(s)	2571, 3290
Csp6I	GTAC	8 cut(s)	867, 1018, 1238, 1430, 1454, 1493, 1529, 1891
CviQI	GTAC	8 cut(s)	867, 1018, 1238, 1430, 1454, 1493, 1529, 1891
DdeI	CTNAG	9 cut(s)	281, 294, 906, 948, 1032, 1227, 1929, 2224, 2337
DinI	GGCGCC	1 cut(s)	622
DpnI	GATC	7 cut(s)	12, 1287, 1801, 2130, 2441, 2863, 2915
DpnII	GATC	7 cut(s)	10, 1285, 1799, 2128, 2439, 2861, 2913
DraIII	CACNNNGTG	1 cut(s)	1189
DrdI	GACNNNNNNGTC	1 cut(s)	307
DseDI	GACNNNNNNGTC	1 cut(s)	307
EaeI	YGGCCR	7 cut(s)	3, 33, 354, 416, 774, 879, 3207
Eam1104I	CTCTTC	4 cut(s)	134, 2160, 2553, 2682
EarI	CTCTTC	4 cut(s)	134, 2160, 2553, 2682
EciI	GGCGGA	1 cut(s)	382
Ecl136II	GAGCTC	2 cut(s)	385, 2215
Eco105I	TACGTA	1 cut(s)	2278
Eco130I	CCWWGG	5 cut(s)	351, 1870, 2120, 2496, 3078
Eco147I	AGGCCT	2 cut(s)	194, 716
Eco24I	GRGCYC	4 cut(s)	387, 925, 2217, 3140
Eco47I	GGWCC	6 cut(s)	52, 178, 679, 780, 994, 1555
Eco53kI	GAGCTC	2 cut(s)	385, 2215
Eco57I	CTGAAG	4 cut(s)	191, 1431, 1904, 2555
Eco72I	CACGTG	1 cut(s)	1298
Eco81I	CCTNAGG	2 cut(s)	281, 2337
Eco88I	CYCGRG	4 cut(s)	55, 206, 857, 926
EcoICRI	GAGCTC	2 cut(s)	385, 2215
EcoNI	CCTNNNNNAGG	4 cut(s)	661, 1250, 1874, 3057
EcoO109I	RGGNCCY	4 cut(s)	124, 285, 679, 3137
EcoRI	GAATTC	1 cut(s)	1273
EcoRII	CCWGG	8 cut(s)	471, 675, 911, 1500, 1557, 2571, 3035, 3290
EcoT14I	CCWWGG	5 cut(s)	351, 1870, 2120, 2496, 3078
EcoT22I	ATGCAT	2 cut(s)	2371, 2429
EcoT38I	GRGCYC	4 cut(s)	387, 925, 2217, 3140
EgeI	GGCGCC	1 cut(s)	622
EheI	GGCGCC	1 cut(s)	622
ErhI	CCWWGG	5 cut(s)	351, 1870, 2120, 2496, 3078
FalI	AAGNNNNNCTT	6 cut(s)	2514, 2546, 2547, 2579, 2733, 2765
FaqI	GGGAC	7 cut(s)	515, 680, 793, 838, 1109, 2262, 3014
FauI	CCCGC	4 cut(s)	221, 284, 540, 3187
FauNDI	CATATG	1 cut(s)	2956
FbaI	TGATCA	2 cut(s)	2128, 2913
FblI	GTMKAC	1 cut(s)	299
FriOI	GRGCYC	4 cut(s)	387, 925, 2217, 3140
FspBI	CTAG	7 cut(s)	527, 1056, 1854, 2043, 2363, 2898, 3225
FspI	TGCGCA	1 cut(s)	498
GlaI	GCGC	8 cut(s)	483, 498, 507, 595, 622, 655, 744, 753
GsaI	CCCAGC	1 cut(s)	3313
GsuI	CTGGAG	5 cut(s)	79, 455, 776, 1419, 2072
HaeII	RGCGCY	4 cut(s)	597, 624, 657, 755
HapII	CCGG	8 cut(s)	50, 155, 238, 419, 585, 777, 927, 2210
HgaI	GACGC	5 cut(s)	167, 436, 840, 1769, 3273
HhaI	GCGC	8 cut(s)	484, 499, 508, 596, 623, 656, 745, 754
Hin1I	GRCGYC	3 cut(s)	159, 600, 621
Hin6I	GCGC	8 cut(s)	482, 497, 506, 594, 621, 654, 743, 752
HinP1I	GCGC	8 cut(s)	482, 497, 506, 594, 621, 654, 743, 752
HincII	GTYRAC	4 cut(s)	300, 540, 1338, 3288
HindII	GTYRAC	4 cut(s)	300, 540, 1338, 3288
HindIII	AAGCTT	1 cut(s)	1520
HinfI	GANTC	7 cut(s)	58, 260, 338, 476, 1595, 1964, 2270
HpaII	CCGG	8 cut(s)	50, 155, 238, 419, 585, 777, 927, 2210
HphI	GGTGA	8 cut(s)	442, 1061, 1283, 1364, 2591, 2897, 2923, 3074
Hpy166II	GTNNAC	7 cut(s)	300, 312, 540, 1020, 1192, 1338, 3288
Hpy8I	GTNNAC	7 cut(s)	300, 312, 540, 1020, 1192, 1338, 3288
Hpy99I	CGWCG	2 cut(s)	260, 602
HpyAV	CCTTC	7 cut(s)	857, 1735, 1920, 2102, 2239, 2516, 2530
HpyCH4III	ACNGT	4 cut(s)	111, 376, 2014, 3002
HpyCH4IV	ACGT	5 cut(s)	600, 1297, 1693, 2277, 2769
HpyF3I	CTNAG	9 cut(s)	281, 294, 906, 948, 1032, 1227, 1929, 2224, 2337
HpySE526I	ACGT	5 cut(s)	600, 1297, 1693, 2277, 2769
Hsp92I	GRCGYC	3 cut(s)	159, 600, 621
HspAI	GCGC	8 cut(s)	482, 497, 506, 594, 621, 654, 743, 752
KasI	GGCGCC	1 cut(s)	620
KpnI	GGTACC	1 cut(s)	1496
KroI	GCCGGC	1 cut(s)	584
KroNI	GCCGGC	1 cut(s)	586
Ksp22I	TGATCA	2 cut(s)	2128, 2913
Kzo9I	GATC	7 cut(s)	10, 1285, 1799, 2128, 2439, 2861, 2913
LguI	GCTCTTC	1 cut(s)	2553
LmnI	GCTCC	9 cut(s)	382, 421, 474, 552, 1400, 1606, 2143, 2212, 2220
MabI	ACCWGGT	2 cut(s)	2571, 3290
MaeI	CTAG	7 cut(s)	527, 1056, 1854, 2043, 2363, 2898, 3225
MaeII	ACGT	5 cut(s)	600, 1297, 1693, 2277, 2769
MaeIII	GTNAC	7 cut(s)	186, 448, 1599, 1839, 1945, 2072, 2452
MalI	GATC	7 cut(s)	12, 1287, 1801, 2130, 2441, 2863, 2915
MboI	GATC	7 cut(s)	10, 1285, 1799, 2128, 2439, 2861, 2913
MfeI	CAATTG	2 cut(s)	806, 1331
MflI	RGATCY	3 cut(s)	1285, 1799, 2861
MhlI	GDGCHC	6 cut(s)	387, 925, 1194, 1226, 2217, 3140
MlsI	TGGCCA	4 cut(s)	35, 356, 881, 3209
MluNI	TGGCCA	4 cut(s)	35, 356, 881, 3209
Mly113I	GGCGCC	1 cut(s)	621
MlyI	GAGTC	4 cut(s)	67, 254, 485, 2279
MmeI	TCCRAC	7 cut(s)	600, 684, 972, 1705, 2165, 2883, 3099
Mox20I	TGGCCA	4 cut(s)	35, 356, 881, 3209
Mph1103I	ATGCAT	2 cut(s)	2371, 2429
MroNI	GCCGGC	1 cut(s)	584
MroXI	GAANNNNTTC	1 cut(s)	1277
MscI	TGGCCA	4 cut(s)	35, 356, 881, 3209
MslI	CAYNNNNRTG	4 cut(s)	977, 1296, 2003, 2736
Msp20I	TGGCCA	4 cut(s)	35, 356, 881, 3209
MspA1I	CMGCKG	2 cut(s)	31, 2195
MspCI	CTTAAG	1 cut(s)	1934
MspI	CCGG	8 cut(s)	50, 155, 238, 419, 585, 777, 927, 2210
MunI	CAATTG	2 cut(s)	806, 1331
Mva1269I	GAATGC	1 cut(s)	2358
MvaI	CCWGG	8 cut(s)	473, 677, 913, 1502, 1559, 2573, 3037, 3292
MvnI	CGCG	1 cut(s)	255
NaeI	GCCGGC	1 cut(s)	586
NarI	GGCGCC	1 cut(s)	621
NciI	CCSGG	5 cut(s)	50, 155, 778, 927, 928
NcoI	CCATGG	2 cut(s)	351, 2120
NdeI	CATATG	1 cut(s)	2956
NdeII	GATC	7 cut(s)	10, 1285, 1799, 2128, 2439, 2861, 2913
NgoMIV	GCCGGC	1 cut(s)	584
NmeAIII	GCCGAG	5 cut(s)	145, 597, 813, 1240, 3205
NmuCI	GTSAC	2 cut(s)	448, 1599
NruI	TCGCGA	1 cut(s)	255
NsbI	TGCGCA	1 cut(s)	498
NsiI	ATGCAT	2 cut(s)	2371, 2429
NspI	RCATGY	2 cut(s)	988, 2080
NspV	TTCGAA	1 cut(s)	66
OliI	CACNNNNGTG	1 cut(s)	1296
PaeR7I	CTCGAG	1 cut(s)	857
PceI	AGGCCT	2 cut(s)	194, 716
PciI	ACATGT	1 cut(s)	984
PciSI	GCTCTTC	1 cut(s)	2553
PcsI	WCGNNNNNNNCGW	1 cut(s)	1690
PctI	GAATGC	1 cut(s)	2358
PdiI	GCCGGC	1 cut(s)	586
PdmI	GAANNNNTTC	1 cut(s)	1277
PfeI	GAWTC	3 cut(s)	338, 1595, 1964
PfoI	TCCNGGA	3 cut(s)	471, 675, 1557
PleI	GAGTC	4 cut(s)	66, 254, 484, 2278
PluTI	GGCGCC	1 cut(s)	624
PmaCI	CACGTG	1 cut(s)	1298
PmlI	CACGTG	1 cut(s)	1298
PpsI	GAGTC	4 cut(s)	66, 254, 484, 2278
Ppu21I	YACGTR	3 cut(s)	1298, 1694, 2278
PpuMI	RGGWCCY	1 cut(s)	679
PscI	ACATGT	1 cut(s)	984
PshAI	GACNNNNGTC	1 cut(s)	57
PshBI	ATTAAT	2 cut(s)	1317, 3237
Psp124BI	GAGCTC	2 cut(s)	387, 2217
Psp5II	RGGWCCY	1 cut(s)	679
Psp6I	CCWGG	8 cut(s)	471, 675, 911, 1500, 1557, 2571, 3035, 3290
PspCI	CACGTG	1 cut(s)	1298
PspFI	CCCAGC	1 cut(s)	3309
PspGI	CCWGG	8 cut(s)	471, 675, 911, 1500, 1557, 2571, 3035, 3290
PspOMI	GGGCCC	1 cut(s)	3136
PspPPI	RGGWCCY	1 cut(s)	679
PspXI	VCTCGAGB	1 cut(s)	857
PstI	CTGCAG	3 cut(s)	1027, 1963, 2686
PstNI	CAGNNNCTG	6 cut(s)	31, 571, 646, 682, 1031, 1592
PsuI	RGATCY	3 cut(s)	1285, 1799, 2861
PvuII	CAGCTG	2 cut(s)	31, 2195
RruI	TCGCGA	1 cut(s)	255
RsaI	GTAC	8 cut(s)	868, 1019, 1239, 1431, 1455, 1494, 1530, 1892
RsaNI	GTAC	8 cut(s)	867, 1018, 1238, 1430, 1454, 1493, 1529, 1891
RseI	CAYNNNNRTG	4 cut(s)	977, 1296, 2003, 2736
SacI	GAGCTC	2 cut(s)	387, 2217
SalI	GTCGAC	1 cut(s)	298
SapI	GCTCTTC	1 cut(s)	2553
Sau3AI	GATC	7 cut(s)	10, 1285, 1799, 2128, 2439, 2861, 2913
ScaI	AGTACT	1 cut(s)	1455
SchI	GAGTC	4 cut(s)	67, 254, 485, 2279
SduI	GDGCHC	6 cut(s)	387, 925, 1194, 1226, 2217, 3140
SexAI	ACCWGGT	2 cut(s)	2571, 3290
SfcI	CTRYAG	5 cut(s)	1023, 1535, 1959, 2064, 2682
SfoI	GGCGCC	1 cut(s)	622
Sfr274I	CTCGAG	1 cut(s)	857
SfuI	TTCGAA	1 cut(s)	66
SinI	GGWCC	6 cut(s)	52, 178, 679, 780, 994, 1555
SlaI	CTCGAG	1 cut(s)	857
SmaI	CCCGGG	1 cut(s)	928
SmiMI	CAYNNNNRTG	4 cut(s)	977, 1296, 2003, 2736
SmlI	CTYRAG	5 cut(s)	305, 857, 998, 1934, 2256
SmoI	CTYRAG	5 cut(s)	305, 857, 998, 1934, 2256
SnaBI	TACGTA	1 cut(s)	2278
SpeI	ACTAGT	2 cut(s)	2042, 3224
SseBI	AGGCCT	2 cut(s)	194, 716
SspDI	GGCGCC	1 cut(s)	620
SspI	AATATT	1 cut(s)	2756
SspMI	CTAG	7 cut(s)	527, 1056, 1854, 2043, 2363, 2898, 3225
SstI	GAGCTC	2 cut(s)	387, 2217
StuI	AGGCCT	2 cut(s)	194, 716
StyI	CCWWGG	5 cut(s)	351, 1870, 2120, 2496, 3078
TaaI	ACNGT	4 cut(s)	111, 376, 2014, 3002
TaiI	ACGT	5 cut(s)	603, 1300, 1696, 2280, 2772
TaqI	TCGA	7 cut(s)	66, 197, 299, 768, 858, 1248, 3229
TaqII	GACCGA	2 cut(s)	544, 797
TatI	WGTACW	3 cut(s)	1429, 1453, 1528
TauI	GCSGC	4 cut(s)	8, 244, 584, 1113
TfiI	GAWTC	3 cut(s)	338, 1595, 1964
TscAI	CASTG	6 cut(s)	449, 572, 647, 1027, 1471, 2316
TseFI	GTSAC	2 cut(s)	448, 1599
Tsp45I	GTSAC	2 cut(s)	448, 1599
TspGWI	ACGGA	4 cut(s)	214, 725, 1137, 1355
TspMI	CCCGGG	1 cut(s)	926
TspRI	CASTG	6 cut(s)	449, 572, 647, 1027, 1471, 2316
Vha464I	CTTAAG	1 cut(s)	1934
VneI	GTGCAC	1 cut(s)	1190
VpaK11BI	GGWCC	6 cut(s)	52, 178, 679, 780, 994, 1555
VspI	ATTAAT	2 cut(s)	1317, 3237
XagI	CCTNNNNNAGG	4 cut(s)	661, 1250, 1874, 3057
XapI	RAATTY	3 cut(s)	1273, 2162, 2346
XbaI	TCTAGA	1 cut(s)	2362
XceI	RCATGY	2 cut(s)	988, 2080
XcmI	CCANNNNNNNNNTGG	2 cut(s)	349, 1302
XhoI	CTCGAG	1 cut(s)	857
XmaI	CCCGGG	1 cut(s)	926
XmiI	GTMKAC	1 cut(s)	299
XmnI	GAANNNNTTC	1 cut(s)	1277
XspI	CTAG	7 cut(s)	527, 1056, 1854, 2043, 2363, 2898, 3225
ZraI	GACGTC	1 cut(s)	601
ZrmI	AGTACT	1 cut(s)	1455
Zsp2I	ATGCAT	2 cut(s)	2371, 2429

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.