Basic Information

Type: gene

Biological Identity

Species

rosa_chinensis

Chromosome

Orthogroup ID

OG0008343

Physical Location & Seq

Genomic Coordinates Forward (+)

12242439 .. 12252868

Gene Structure 10430 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

PRQ36853

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2583 bp

ATGATGGCTTCTCTCCACACCCACCACTACCATCTTCATCTCCGCCGCTCTACCCCCTCCAAACTCCACACCCATCAAGCCCCCATATTCTCACGGCGTTTCCCGACCGGAAAACAAAGGGCACTTACTCGTGGACAGAACAACCGCAAGGGTGGTAGAGATGGAACAACTGACTCGTTCTGCTGGGTGTGCAGAGCTGGTTTGGAGATTGATAAGGTCTCGCCTGATGATGAAGGAAGTGAAAGGCCTGCTTTTGATATCAATTTGGCCGTCATTCTCGCCGGTTTCGCTTTCGAGGCCTATTCTAGCCCACCTGCTAATGTTGGAAGGCGTGAAATTGATGCTGCAGATTGTAAGACAGTGTACCTTTCAGAGTCATTTGTGCGTGAGATATACGATGGCCAACTATCCGTGAAATTGAAAAAAGGCGTCAATCTTCCTGCCATGGATCCATGGGGAACAAGTGATCCATATGTGATCATGCAATTAGATGGTCAAGTTAATAAAAGCAAGATTAAATGGGGGACAAAAGAACCAACTTGGAACGAGGACTTCAATTTTAATATCAAGCAGCCTCCAACCAAAAATCTTCAGGTTGCTGCTTGGGATGCAAACCTTGTAACTCCACATAAGCGCATGGGGAATGCAGGCATCAATCTAGAATGCCTTTGTGATGGGAATTTGCATGAAATACTGGTGGAATTGGAAGGAATGGGAGGTGGTGGGAAATTACTTATAGAGGTCAGATATGAAACTTTTGAAGAAATTGATGAGGGGAAAAAGTGGTGGATGAGGGTCCCCATTGTTTCTGAATTTCTCCGTAATAAGGGTTTTGAACCTGCTCTGAAAAAGTTTGTTGGCTCTGACACTGTGCAAGCACGTCAGTTTGCAGAATATGCTTTTGGGCAGTTAAAGTCATTCAATAATAATGCTTACCTTTGGAAGAATCTGATTTCAAGTAATGATGAAAGTGATAGGAAGGGCCCTGGGAAACCTAATAATTCTGCTGTTGTGTCAGGCGTGCCTTCCCAGATGGACGGTATCGCAGAGGGTTCTTTAGATAATGCAGACTGCAATGAAGTGAGCAATTTAGATGAGTCTAACAGAGATAATGGTGATGTGGAGAATGGAAATGCCTCTCAGCCAGTGAAAAAACTCGATGATGAAATGCAGTCAGATAAGAACTTTTGGAAGAACTTTGCTAATGAGATCAATCAAAATGTTGTAGAGAAATTTGGATTGCCAGTGCCAGGAAAATTAAAGTGGGATGGGTTTGATTTATTAGACAGAGTTGGTTTGCAGTCACAAAAGATAGCAGAAGCAACTTATGTTGAATCAGGGCTTGCAACTCCAGAAGTGTTAGATGTTGATAACGATAAAACAACAGCCCCACTTCCCATTAGCATTATGCAGTCTTCATTTCCAGATATAAAGGAAGCAACAAGGGAACTATTAAAGCAAACTGATTCTGTTTTAGGAATATTAATGGTTCTAACTGCAACAGTATCTCAAGCAAAGAAGGAAGAAAATATTGTAGGAGGGAGTGCAACTAAGGAAGAGGATGATGTCGTAACTGAGAAGCTTGCTAACTCACAGGGAGCCGAGGAGATGAAAGCACTGTTCTTAACTGCAGAAACTGCCATGGAGGCTTGGGCGATGCTCGCTACTTCTTTAGGACATCCAAGTTTTATAAAGTCTGAATTTGAGAAACTATGTTTCTTAGATAATGAGACAACAGACACACAGGTTGCAATTTGGCGTGATTCTGCACGGAAAAGATTAGTGATCGCTTTCAGGGGAACAGAGCAGGCAAGATGGAAGGACTTGCGAACGGATTTGATGCTAGCTCCTGCTGGGCTGAATCCAGAAAGGATTGGAGGAGACTTTAAGCAAGAAATTCAAGTTCATAGTGGCTTCTTAGGTGCATATGATTCTGTCAGAATAAGGATCATCTCTCTAATTAAATTAGCAATCGGTTATATTGATGATGTTGCTGAGCCGGTGCACAGATGGCATGTTTATGTAACTGGTCATAGTCTTGGTGGTGCATTGGCTACTCTCCTAGCTCTCGAACTTGCATCAAGTCAATTAGCAAAGCATGGAGTGATCACTATAAGCATGTATAATTTCGGATCTCCTAGGGTTGGGAACAAAAGATTTGCTGATGTTTATAATGAGAAAGTCAAAGATAGTTGGAGAGTTGTAAATCACAGAGACATTATACCAACTGTGCCCCGCTTGATGGGTTACTGCCATGTAGCTCAACCTGTTTATCTAGCAACAGGAGACCTAGCGAATGCCTTGGAAACTATGGAGCTCTCTGGAGATGGTTACCAAGCTGATATGATCGGGGAGTCCACACCGGATGTTCTCGTCAGTGAATTCATGAAGGGTGAGAAGGAACTTATACAGAAAATTTTACAGACTGAAATAAATATATTCCGTTCAATCAGAGATGGAACTGCACTTATGCAACATATGGAAGATTTTTACTACATCACGTTGCTTGAGAATGTGAGATCAAATTACCAACCTGTTGCCAAGTCGTTATCCGACGAACAATCCAACAAAACAATCAGCTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001505 GO:0003674 GO:0005488 GO:0005509 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005783 GO:0005789 GO:0007154 GO:0007165 GO:0008021 GO:0008150 GO:0009987 GO:0010646 GO:0012505 GO:0012506 GO:0016020 GO:0016021 GO:0019722 GO:0019932 GO:0023051 GO:0023052 GO:0030100 GO:0030133 GO:0030334 GO:0030336 GO:0030658 GO:0030659 GO:0030672 GO:0031090 GO:0031224 GO:0031410 GO:0031982 GO:0031984 GO:0032879 GO:0035556 GO:0040012 GO:0040013 GO:0042175 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044425 GO:0044432 GO:0044433 GO:0044444 GO:0044446 GO:0044456 GO:0044464 GO:0045202 GO:0045806 GO:0046872 GO:0046928 GO:0048167 GO:0048168 GO:0048519 GO:0048523 GO:0048583 GO:0048585 GO:0050789 GO:0050794 GO:0050804 GO:0050896 GO:0051046 GO:0051049 GO:0051051 GO:0051128 GO:0051129 GO:0051270 GO:0051271 GO:0051588 GO:0051716 GO:0055037 GO:0060341 GO:0060627 GO:0065007 GO:0065008 GO:0070382 GO:0080134 GO:0097458 GO:0097708 GO:0098588 GO:0098793 GO:0098805 GO:0098827 GO:0099177 GO:0099501 GO:0099503 GO:1902882 GO:1902883 GO:1903530 GO:2000145 GO:2000146

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

C2 Lipase_3

Protein Analysis

860

Amino Acids

95.77

Weight (kDa)

5.4

Isoelectric Point (pI)

40.06

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
C2	PF00168	134 - 229	1.3e-19	C2 domain
Lipase_3	PF01764	595 - 746	4.6e-28	Lipase (class 3)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008343)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G13550 AT4G13550 AT4G13550
fragaria_vesca	FvH4_4g05250 FvH4_4g05250
malus_domestica	MD13G1226600.v1.1
prunus_persica	Prupe.1G054000_v2.0.a1 Prupe.1G054000_v2.0.a1 Prupe.1G054000_v2.0.a1 Prupe.1G054000_v2.0.a1 Prupe.1G054000_v2.0.a1
pyrus_communis	pycom13g19960
rosa_chinensis	RchiOBHm_Chr4g0396161
rosa_laevigata	RLG00000009564
rosa_multiflora	Rmu_sc0006325.1_g000041
rosa_roxburghii	Rroxscaffold_5G00341010
rosa_rugosa	Rorug03G0351200 Rorug03G0351300 Rorug03G0351300
rosa_samantha	Rh4BG065200 Rh4CG072000 Rh4DG062000
rosa_wichuraiana	Rw4G005390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1691, 2172
AarI	CACCTGC	1 cut(s)	322
Acc36I	ACCTGC	2 cut(s)	322, 847
AccBSI	CCGCTC	1 cut(s)	48
AciI	CCGC	4 cut(s)	43, 46, 145, 2236
AclWI	GGATC	5 cut(s)	443, 456, 461, 1955, 2140
AcoI	YGGCCR	2 cut(s)	267, 400
AcsI	RAATTY	7 cut(s)	679, 812, 1232, 1700, 1896, 2381, 2415
AcuI	CTGAAG	1 cut(s)	575
AcyI	GRCGYC	1 cut(s)	429
AfaI	GTAC	1 cut(s)	365
AfiI	CCNNNNNNNGG	4 cut(s)	826, 1250, 2144, 2242
AgsI	TTSAA	9 cut(s)	421, 556, 761, 836, 922, 957, 1334, 1901, 2448
AjiI	CACGTC	1 cut(s)	881
AjnI	CCWGG	2 cut(s)	985, 1249
AjuI	GAANNNNNNNTTGG	2 cut(s)	885, 917
AloI	GAACNNNNNNTCC	2 cut(s)	451, 483
AluBI	AGCT	8 cut(s)	197, 1582, 1847, 2066, 2261, 2317, 2339, 2580
AluI	AGCT	8 cut(s)	197, 1582, 1847, 2066, 2261, 2317, 2339, 2580
Alw21I	GWGCWC	2 cut(s)	2007, 2319
Alw26I	GTCTC	5 cut(s)	223, 1724, 1875, 2208, 2280
Alw44I	GTGCAC	1 cut(s)	2003
AlwI	GGATC	5 cut(s)	443, 456, 461, 1955, 2140
AlwNI	CAGNNNCTG	1 cut(s)	1637
AoxI	GGCC	5 cut(s)	245, 267, 297, 400, 982
ApaI	GGGCCC	1 cut(s)	986
ApaLI	GTGCAC	1 cut(s)	2003
ApeKI	GCWGC	3 cut(s)	344, 571, 599
ApoI	RAATTY	7 cut(s)	679, 812, 1232, 1700, 1896, 2381, 2415
AseI	ATTAAT	1 cut(s)	1484
AspA2I	CCTAGG	1 cut(s)	2138
AspLEI	GCGC	1 cut(s)	636
AspS9I	GGNCC	3 cut(s)	796, 982, 983
AsuHPI	GGTGA	2 cut(s)	1127, 2405
AsuNHI	GCTAGC	1 cut(s)	1843
AvaII	GGWCC	1 cut(s)	796
AvrII	CCTAGG	1 cut(s)	2138
BaeGI	GKGCMC	4 cut(s)	124, 986, 2007, 2235
BalI	TGGCCA	1 cut(s)	402
BamHI	GGATCC	1 cut(s)	448
BanII	GRGCYC	2 cut(s)	986, 2319
BauI	CACGAG	1 cut(s)	129
BbsI	GAAGAC	1 cut(s)	1407
Bbv12I	GWGCWC	2 cut(s)	2007, 2319
BbvI	GCAGC	3 cut(s)	331, 583, 586
BceAI	ACGGC	2 cut(s)	110, 254
BciT130I	CCWGG	2 cut(s)	987, 1251
BclI	TGATCA	2 cut(s)	477, 2106
BcoDI	GTCTC	5 cut(s)	223, 1724, 1875, 2208, 2280
BfaI	CTAG	7 cut(s)	306, 659, 1844, 2063, 2139, 2276, 2291
BfmI	CTRYAG	2 cut(s)	345, 1629
BfuAI	ACCTGC	2 cut(s)	322, 847
BglI	GCCNNNNNGGC	1 cut(s)	1646
BisI	GCNGC	4 cut(s)	46, 345, 572, 600
BlnI	CCTAGG	1 cut(s)	2138
BlpI	GCTNAGC	1 cut(s)	1995
BlsI	GCNGC	4 cut(s)	47, 346, 573, 601
Bme1390I	CCNGG	2 cut(s)	987, 1251
Bme18I	GGWCC	1 cut(s)	796
BmgBI	CACGTC	1 cut(s)	881
BmgT120I	GGNCC	3 cut(s)	796, 982, 983
BmiI	GGNNCC	5 cut(s)	450, 797, 798, 984, 1600
BmrFI	CCNGG	2 cut(s)	987, 1251
BmsI	GCATC	6 cut(s)	331, 598, 660, 1647, 1830, 2087
BmtI	GCTAGC	1 cut(s)	1847
BpiI	GAAGAC	1 cut(s)	1407
BpmI	CTGGAG	2 cut(s)	1335, 2343
Bpu1102I	GCTNAGC	1 cut(s)	1995
BpuEI	CTTGAG	2 cut(s)	1494, 2528
BsaHI	GRCGYC	1 cut(s)	429
BsaI	GGTCTC	2 cut(s)	223, 2280
BsaJI	CCNNGG	8 cut(s)	444, 452, 985, 986, 1602, 1641, 2138, 2301
BsaWI	WCCGGW	2 cut(s)	107, 2362
Bsc4I	CCNNNNNNNGG	4 cut(s)	826, 1250, 2144, 2242
Bse118I	RCCGGY	2 cut(s)	281, 1999
Bse1I	ACTGG	4 cut(s)	699, 1145, 1244, 2032
Bse3DI	GCAATG	1 cut(s)	1081
BseBI	CCWGG	2 cut(s)	987, 1251
BseDI	CCNNGG	8 cut(s)	444, 452, 985, 986, 1602, 1641, 2138, 2301
BseGI	GGATG	6 cut(s)	613, 795, 1273, 1567, 1678, 2371
BseLI	CCNNNNNNNGG	4 cut(s)	826, 1250, 2144, 2242
BseMI	GCAATG	1 cut(s)	1081
BseMII	CTCAG	3 cut(s)	1154, 1566, 1986
BseNI	ACTGG	4 cut(s)	699, 1145, 1244, 2032
BseRI	GAGGAG	2 cut(s)	1619, 1893
BseSI	GKGCMC	4 cut(s)	124, 986, 2007, 2235
BseXI	GCAGC	3 cut(s)	331, 583, 586
BseYI	CCCAGC	2 cut(s)	183, 1853
BsgI	GTGCAG	3 cut(s)	211, 1752, 2448
Bsh1285I	CGRYCG	1 cut(s)	108
BshFI	GGCC	5 cut(s)	247, 269, 299, 402, 984
BsiEI	CGRYCG	1 cut(s)	108
BsiHKAI	GWGCWC	2 cut(s)	2007, 2319
BsiSI	CCGG	4 cut(s)	108, 282, 2000, 2363
BslFI	GGGAC	2 cut(s)	538, 782
BslI	CCNNNNNNNGG	4 cut(s)	826, 1250, 2144, 2242
BsmAI	GTCTC	5 cut(s)	223, 1724, 1875, 2208, 2280
BsmFI	GGGAC	2 cut(s)	538, 782
BsmI	GAATGC	3 cut(s)	649, 668, 2302
BsnI	GGCC	5 cut(s)	247, 269, 299, 402, 984
Bso31I	GGTCTC	2 cut(s)	223, 2280
Bsp120I	GGGCCC	1 cut(s)	982
Bsp1286I	GDGCHC	5 cut(s)	124, 986, 2007, 2235, 2319
Bsp1720I	GCTNAGC	1 cut(s)	1995
Bsp19I	CCATGG	3 cut(s)	444, 452, 1641
BspACI	CCGC	4 cut(s)	43, 46, 145, 2236
BspANI	GGCC	5 cut(s)	247, 269, 299, 402, 984
BspCNI	CTCAG	3 cut(s)	1153, 1567, 1987
BspHI	TCATGA	1 cut(s)	2385
BspLI	GGNNCC	5 cut(s)	450, 797, 798, 984, 1600
BspMAI	CTGCAG	2 cut(s)	349, 1633
BspMI	ACCTGC	2 cut(s)	322, 847
BspOI	GCTAGC	1 cut(s)	1847
BspPI	GGATC	5 cut(s)	443, 456, 461, 1955, 2140
BspTNI	GGTCTC	2 cut(s)	223, 2280
BsrBI	CCGCTC	1 cut(s)	48
BsrDI	GCAATG	1 cut(s)	1081
BsrFI	RCCGGY	2 cut(s)	281, 1999
BsrI	ACTGG	4 cut(s)	699, 1145, 1244, 2032
BssAI	RCCGGY	2 cut(s)	281, 1999
BssECI	CCNNGG	8 cut(s)	444, 452, 985, 986, 1602, 1641, 2138, 2301
BssNI	GRCGYC	1 cut(s)	429
BssSI	CACGAG	1 cut(s)	129
BssT1I	CCWWGG	5 cut(s)	444, 452, 1641, 2138, 2301
Bst2BI	CACGAG	1 cut(s)	129
Bst2UI	CCWGG	2 cut(s)	987, 1251
Bst4CI	ACNGT	6 cut(s)	361, 871, 1040, 1504, 1620, 2230
Bst6I	CTCTTC	1 cut(s)	1551
BstACI	GRCGYC	1 cut(s)	429
BstAPI	GCANNNNNTGC	2 cut(s)	896, 1637
BstC8I	GCNNGC	9 cut(s)	249, 649, 876, 1022, 1344, 1584, 1662, 1809, 1845
BstDEI	CTNAG	6 cut(s)	1140, 1551, 1575, 1720, 1918, 1995
BstDSI	CCRYGG	3 cut(s)	444, 452, 1641
BstEII	GGTNACC	1 cut(s)	2330
BstF5I	GGATG	6 cut(s)	613, 795, 1273, 1567, 1678, 2371
BstHHI	GCGC	1 cut(s)	636
BstMAI	GTCTC	5 cut(s)	223, 1724, 1875, 2208, 2280
BstMCI	CGRYCG	1 cut(s)	108
BstMWI	GCNNNNNNNGC	9 cut(s)	189, 287, 296, 608, 896, 1637, 1646, 1661, 2011
BstNI	CCWGG	2 cut(s)	987, 1251
BstNSI	RCATGY	2 cut(s)	2018, 2122
BstPI	GGTNACC	1 cut(s)	2330
BstSCI	CCNGG	2 cut(s)	985, 1249
BstSFI	CTRYAG	2 cut(s)	345, 1629
BstSLI	GKGCMC	4 cut(s)	124, 986, 2007, 2235
BstV1I	GCAGC	3 cut(s)	331, 583, 586
BstV2I	GAAGAC	1 cut(s)	1407
BstX2I	RGATCY	2 cut(s)	448, 2132
BstYI	RGATCY	2 cut(s)	448, 2132
BsuRI	GGCC	5 cut(s)	247, 269, 299, 402, 984
BtgI	CCRYGG	3 cut(s)	444, 452, 1641
BtgZI	GCGATG	1 cut(s)	1670
BtrI	CACGTC	1 cut(s)	881
BtsCI	GGATG	6 cut(s)	613, 795, 1273, 1567, 1678, 2371
BtsIMutI	CAGTG	6 cut(s)	366, 867, 1152, 1251, 1616, 2383
BveI	ACCTGC	2 cut(s)	322, 847
Cac8I	GCNNGC	9 cut(s)	249, 649, 876, 1022, 1344, 1584, 1662, 1809, 1845
CaiI	CAGNNNCTG	1 cut(s)	1637
CciI	TCATGA	1 cut(s)	2385
CfoI	GCGC	1 cut(s)	636
Cfr10I	RCCGGY	2 cut(s)	281, 1999
Cfr13I	GGNCC	3 cut(s)	796, 982, 983
CseI	GACGC	1 cut(s)	418
Csp6I	GTAC	1 cut(s)	364
CviQI	GTAC	1 cut(s)	364
DdeI	CTNAG	6 cut(s)	1140, 1551, 1575, 1720, 1918, 1995
EaeI	YGGCCR	2 cut(s)	267, 400
Eam1104I	CTCTTC	1 cut(s)	1551
EarI	CTCTTC	1 cut(s)	1551
EciI	GGCGGA	1 cut(s)	32
Ecl136II	GAGCTC	1 cut(s)	2317
Eco130I	CCWWGG	5 cut(s)	444, 452, 1641, 2138, 2301
Eco147I	AGGCCT	2 cut(s)	247, 299
Eco24I	GRGCYC	2 cut(s)	986, 2319
Eco31I	GGTCTC	2 cut(s)	223, 2280
Eco32I	GATATC	1 cut(s)	259
Eco47I	GGWCC	1 cut(s)	796
Eco53kI	GAGCTC	1 cut(s)	2317
Eco57I	CTGAAG	1 cut(s)	575
Eco91I	GGTNACC	1 cut(s)	2330
EcoICRI	GAGCTC	1 cut(s)	2317
EcoO109I	RGGNCCY	3 cut(s)	796, 982, 983
EcoO65I	GGTNACC	1 cut(s)	2330
EcoRI	GAATTC	1 cut(s)	2381
EcoRII	CCWGG	2 cut(s)	985, 1249
EcoRV	GATATC	1 cut(s)	259
EcoT14I	CCWWGG	5 cut(s)	444, 452, 1641, 2138, 2301
EcoT38I	GRGCYC	2 cut(s)	986, 2319
ErhI	CCWWGG	5 cut(s)	444, 452, 1641, 2138, 2301
FalI	AAGNNNNNCTT	2 cut(s)	235, 267
FaqI	GGGAC	2 cut(s)	538, 782
FauI	CCCGC	1 cut(s)	2243
FauNDI	CATATG	3 cut(s)	472, 1927, 2478
FbaI	TGATCA	2 cut(s)	477, 2106
Fnu4HI	GCNGC	4 cut(s)	46, 345, 572, 600
FokI	GGATG	6 cut(s)	620, 802, 1280, 1574, 1665, 2378
FriOI	GRGCYC	2 cut(s)	986, 2319
Fsp4HI	GCNGC	4 cut(s)	46, 345, 572, 600
FspBI	CTAG	7 cut(s)	306, 659, 1844, 2063, 2139, 2276, 2291
GlaI	GCGC	1 cut(s)	635
GluI	GCNGC	4 cut(s)	46, 345, 572, 600
GsaI	CCCAGC	2 cut(s)	187, 1857
GsuI	CTGGAG	2 cut(s)	1335, 2343
HaeIII	GGCC	5 cut(s)	247, 269, 299, 402, 984
HapII	CCGG	4 cut(s)	108, 282, 2000, 2363
HgaI	GACGC	1 cut(s)	418
HhaI	GCGC	1 cut(s)	636
Hin1I	GRCGYC	1 cut(s)	429
Hin6I	GCGC	1 cut(s)	634
HinP1I	GCGC	1 cut(s)	634
HindIII	AAGCTT	1 cut(s)	1580
HpaII	CCGG	4 cut(s)	108, 282, 2000, 2363
HphI	GGTGA	2 cut(s)	1127, 2405
Hpy166II	GTNNAC	4 cut(s)	134, 364, 2005, 2358
Hpy188III	TCNNGA	8 cut(s)	103, 659, 1352, 1424, 1865, 2069, 2322, 2386
Hpy8I	GTNNAC	4 cut(s)	134, 364, 2005, 2358
Hpy99I	CGWCG	1 cut(s)	2558
HpyAV	CCTTC	9 cut(s)	227, 321, 701, 973, 1035, 1513, 1813, 2383, 2392
HpyCH4III	ACNGT	6 cut(s)	361, 871, 1040, 1504, 1620, 2230
HpyCH4IV	ACGT	2 cut(s)	880, 2501
HpyF10VI	GCNNNNNNNGC	9 cut(s)	189, 287, 296, 608, 896, 1637, 1646, 1661, 2011
HpyF3I	CTNAG	6 cut(s)	1140, 1551, 1575, 1720, 1918, 1995
HpySE526I	ACGT	2 cut(s)	880, 2501
Hsp92I	GRCGYC	1 cut(s)	429
HspAI	GCGC	1 cut(s)	634
KflI	GGGWCCC	1 cut(s)	796
Ksp22I	TGATCA	2 cut(s)	477, 2106
LmnI	GCTCC	3 cut(s)	1598, 1852, 2314
Lsp1109I	GCAGC	3 cut(s)	331, 583, 586
LweI	GCATC	6 cut(s)	331, 598, 660, 1647, 1830, 2087
MaeI	CTAG	7 cut(s)	306, 659, 1844, 2063, 2139, 2276, 2291
MaeII	ACGT	2 cut(s)	880, 2501
MaeIII	GTNAC	6 cut(s)	619, 1302, 1570, 2023, 2246, 2330
MbiI	CCGCTC	1 cut(s)	48
MflI	RGATCY	2 cut(s)	448, 2132
MhlI	GDGCHC	5 cut(s)	124, 986, 2007, 2235, 2319
MlsI	TGGCCA	1 cut(s)	402
MluNI	TGGCCA	1 cut(s)	402
MlyI	GAGTC	4 cut(s)	167, 383, 1106, 2363
MmeI	TCCRAC	4 cut(s)	304, 602, 2174, 2577
Mox20I	TGGCCA	1 cut(s)	402
MscI	TGGCCA	1 cut(s)	402
MslI	CAYNNNNRTG	1 cut(s)	2019
Msp20I	TGGCCA	1 cut(s)	402
MspA1I	CMGCKG	1 cut(s)	2580
MspI	CCGG	4 cut(s)	108, 282, 2000, 2363
MspR9I	CCNGG	2 cut(s)	987, 1251
Mva1269I	GAATGC	3 cut(s)	649, 668, 2302
MvaI	CCWGG	2 cut(s)	987, 1251
MwoI	GCNNNNNNNGC	9 cut(s)	189, 287, 296, 608, 896, 1637, 1646, 1661, 2011
NcoI	CCATGG	3 cut(s)	444, 452, 1641
NdeI	CATATG	3 cut(s)	472, 1927, 2478
NheI	GCTAGC	1 cut(s)	1843
NlaIV	GGNNCC	5 cut(s)	450, 797, 798, 984, 1600
NmeAIII	GCCGAG	1 cut(s)	1627
NmuCI	GTSAC	1 cut(s)	1302
NspI	RCATGY	2 cut(s)	2018, 2122
PagI	TCATGA	1 cut(s)	2385
PaqCI	CACCTGC	1 cut(s)	322
PasI	CCCWGGG	1 cut(s)	986
PceI	AGGCCT	2 cut(s)	247, 299
PctI	GAATGC	3 cut(s)	649, 668, 2302
PfeI	GAWTC	6 cut(s)	946, 1334, 1466, 1763, 1861, 1931
PkrI	GCNGC	4 cut(s)	47, 346, 573, 601
PleI	GAGTC	4 cut(s)	167, 382, 1105, 2362
PpsI	GAGTC	4 cut(s)	167, 382, 1105, 2362
PpuMI	RGGWCCY	1 cut(s)	796
PshBI	ATTAAT	1 cut(s)	1484
PsiI	TTATAA	2 cut(s)	1691, 2172
Psp124BI	GAGCTC	1 cut(s)	2319
Psp5II	RGGWCCY	1 cut(s)	796
Psp6I	CCWGG	2 cut(s)	985, 1249
PspEI	GGTNACC	1 cut(s)	2330
PspFI	CCCAGC	2 cut(s)	183, 1853
PspGI	CCWGG	2 cut(s)	985, 1249
PspN4I	GGNNCC	5 cut(s)	450, 797, 798, 984, 1600
PspOMI	GGGCCC	1 cut(s)	982
PspPI	GGNCC	3 cut(s)	796, 982, 983
PspPPI	RGGWCCY	1 cut(s)	796
PstI	CTGCAG	2 cut(s)	349, 1633
PstNI	CAGNNNCTG	1 cut(s)	1637
PsuI	RGATCY	2 cut(s)	448, 2132
PvuII	CAGCTG	1 cut(s)	2580
RsaI	GTAC	1 cut(s)	365
RsaNI	GTAC	1 cut(s)	364
RseI	CAYNNNNRTG	1 cut(s)	2019
SacI	GAGCTC	1 cut(s)	2319
SatI	GCNGC	4 cut(s)	46, 345, 572, 600
Sau96I	GGNCC	3 cut(s)	796, 982, 983
SchI	GAGTC	4 cut(s)	167, 383, 1106, 2363
ScrFI	CCNGG	2 cut(s)	987, 1251
SduI	GDGCHC	5 cut(s)	124, 986, 2007, 2235, 2319
SfaNI	GCATC	6 cut(s)	331, 598, 660, 1647, 1830, 2087
SfcI	CTRYAG	2 cut(s)	345, 1629
SinI	GGWCC	1 cut(s)	796
SmiMI	CAYNNNNRTG	1 cut(s)	2019
SmlI	CTYRAG	2 cut(s)	1509, 2507
SmoI	CTYRAG	2 cut(s)	1509, 2507
SseBI	AGGCCT	2 cut(s)	247, 299
SsiI	CCGC	4 cut(s)	43, 46, 145, 2236
SspI	AATATT	2 cut(s)	1482, 1531
SspMI	CTAG	7 cut(s)	306, 659, 1844, 2063, 2139, 2276, 2291
SstI	GAGCTC	1 cut(s)	2319
StuI	AGGCCT	2 cut(s)	247, 299
StyD4I	CCNGG	2 cut(s)	985, 1249
StyI	CCWWGG	5 cut(s)	444, 452, 1641, 2138, 2301
TaaI	ACNGT	6 cut(s)	361, 871, 1040, 1504, 1620, 2230
TaiI	ACGT	2 cut(s)	883, 2504
TaqI	TCGA	3 cut(s)	294, 1158, 2070
TauI	GCSGC	1 cut(s)	48
TfiI	GAWTC	6 cut(s)	946, 1334, 1466, 1763, 1861, 1931
TscAI	CASTG	6 cut(s)	366, 874, 1152, 1251, 1623, 2383
TseFI	GTSAC	1 cut(s)	1302
TseI	GCWGC	3 cut(s)	344, 571, 599
Tsp45I	GTSAC	1 cut(s)	1302
TspGWI	ACGGA	5 cut(s)	400, 809, 1786, 1847, 2432
TspRI	CASTG	6 cut(s)	366, 874, 1152, 1251, 1623, 2383
VneI	GTGCAC	1 cut(s)	2003
VpaK11BI	GGWCC	1 cut(s)	796
VspI	ATTAAT	1 cut(s)	1484
XapI	RAATTY	7 cut(s)	679, 812, 1232, 1700, 1896, 2381, 2415
XbaI	TCTAGA	1 cut(s)	658
XceI	RCATGY	2 cut(s)	2018, 2122
XmaJI	CCTAGG	1 cut(s)	2138
XspI	CTAG	7 cut(s)	306, 659, 1844, 2063, 2139, 2276, 2291

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.