Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr13

Orthogroup ID

OG0008944

Physical Location & Seq

Genomic Coordinates Forward (+)

32870798 .. 32879996

Gene Structure 9199 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD13G1261400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2169 bp

ATGGAATTGAGGAAGGAAAGGCTTGTCAGGTTTTATTCTGATAAAAAACGGCATAAAGAATCTTTATGGCGAAGAGCTGAACCATCTCAGCTTGAAAAGTCATCGTCTGCATATAAGTCATCTTCATTGCTTAAATCTGACACTGGACTAGTGGGGGGAAGAAATAGGTTTGTTGAAACTTTCAGCATTGGCCATTCAAAGGTGTTTCCAGAAGATCATGAACCTTGGCGAAAGAGAATACTTGACCCGGGTAGTGAGATCATGTTGCAATGGAATCGGGTTTTCATTGTCTCGTGCTTGGTGGCACTTTTTGTTGACCCCTTGTATTTTTACTTGCCCATGCTGGGAGGGGATGCAAACACGTATTGTGTGAAGACAGACTTGAGGTTGCGAATTGTTGTGACTTGTTTCCGGACTATTGCGGATATCTTTTATATGTTGCATGTGATGATAAAGTTTAGGACAGCTTATGTTGCGCCAAGCTCTCGCGTTTTTGGGAGGGGTGAACTTGTCATGGACCCAAAGAAGATTGCGCTGAGGTATATTAGATCAGACTTCTTCATTGATCTCATAGCCACATTGCCCCTCCCTCAGATAGTCATCTGGTTTATTATCCCCGCAACAAGAAGCGCCCAAACGGATCATAACAACAATGCTCTTGCTCTAATTGTTCTGATCCAATATGTCCCCAGATTATATCTTATTTTTCCATTAAGTTTGCAAATAATCAAAGCAACTGGAGTGGTCACAAAGACTGCCTGGGCAGGGGCTGCATATAATCTTCTATTGTACATGCTGGCCAGCCATGTGTTAGGGGCAGCATGGTATTTACTATCAATTGACCGATATACCTCATGCTGGAAGTCATTTTGTAAAAAGGAACTGAACACCACAAAATGTTCGCTTTCTTATCTAGATTGTGACAATCTTAGCAGAAAAGGTTGGGCAAGTAGTACAAATGTGTTTGAGAATTGTAATCCTGCAAATGACAATTTCAAATATGGCATATTCGAAAGCGCAGTAACAAAAAATGTCGTTTCCTCAAACTTCATCATGAAGTACTTCTATTGTTTATGGTGGGGCTTGCAACAACTAAGTTCCTATGGGCAGAATTTGTCAACAAGTACTTTTATTGGGGAAACGTCATTTGCCATTCTCATTGCCATTGTGGGCCTTGTATTATTTGCCCACTTAATCGGGAATATGCAGACCTATCTCCAATCAATCACTGTGAGGCTTGAGGAGTGGAGGCTTAAGAGAAGAGACACAGAGGAATGGATGAAGCATCGTCAACTCCCTGAAGATTTGAGACGACGTGTTCGACGCTTTGTTCAGTACAAATGGCTGGCAACTAGAGGAGTTGATGAAGAATCCATCCTTCATGGCTTACCTGCTGATCTTCGTCGTGATATTCAACGCCACCTATGCTTAGATCTTGTTCGAAGAGTCCCTTTCTTCTCACAGATGGATGATCAGCTTCTCGATGCAATATGTGAGCGTTTGGTGTCCTCCCTAAGCACGGGGGGTACTTACATAGTCCGAGAGGGTGACCCAGTGTCAGAAATGCTTTTCATTATAAGAGGGACTCTGGATAGTTCTACGACCAATGGAGGGCGGACTGGATTCTTCAATTCAATCACATTAAGACCTGGAGACTTTTGTGGTGAGGAGCTGCTCGCATGGGCTTTGCTCCCAAAATCCACTGCTAACTTGCCTTCTTCAACACGAACAGTTAAAGCACTTAGTGAAGTTGAAGCTTTTGCATTACGAGCTGAAGATCTCAAATTTGTGGCTAACCAATTCAGACGCCTGCACAGCAAGAAGCTGCAGCACACATTTCGGTTTTACTCTCACCATTGGAGAACATGGGCTGCCTGCTTCATACAGGCTGCTTGGCGCCGCCACAAGAGAAGAATGACGACAAAAGATCTGAGCATGAGGGAATCATTTGCTTTGGATGAGAAAGTGGCTTATGAGACTGAGCAAGCGGAAGAAGATCTTTCTGTTGTTTCAAATCCCTCTTCCCAAGCAAAAATGAATCTTAGGGTCACAGTACTGGCTTCAAGATTTGCAGCAAACACAAGAAGGGGAGCTCAGAAGCTCAAAGACGAGATGCCAAAATTACGAAAACCTGAAGAACCGGACTTTTCCACTGAGCCAGAAGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042391 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

Ion_trans cNMP_binding

Protein Analysis

723

Amino Acids

83.3

Weight (kDa)

9.43

Isoelectric Point (pI)

48.81

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	91 - 412	7.7e-18	Ion transport protein
cNMP_binding	PF00027	509 - 597	2.5e-07	Cyclic nucleotide-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008944)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G30360
fragaria_vesca	FvH4_4g09800
malus_domestica	MD13G1261400.v1.1 MD16G1264100.v1.1
prunus_persica	Prupe.1G095400_v2.0.a1 Prupe.1G095400_v2.0.a1 Prupe.1G095400_v2.0.a1 Prupe.1G095400_v2.0.a1 Prupe.1G095400_v2.0.a1
pyrus_communis	pycom13g24930 pycom16g23260
rosa_chinensis	RchiOBHm_Chr4g0404621
rosa_laevigata	RLG00000008918
rosa_multiflora	Rmu_sc0002699.1_g000026
rosa_roxburghii	Rroxscaffold_5G00348770
rosa_rugosa	Rorug04G0048000
rosa_samantha	Rh4AG124000 Rh4BG117700 Rh4BG117800 Rh4CG132100 Rh4DG116900
rosa_wichuraiana	Rw4G010050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1577
Acc36I	ACCTGC	1 cut(s)	1399
AccB1I	GGYRCC	1 cut(s)	1896
AccII	CGCG	1 cut(s)	489
AccIII	TCCGGA	1 cut(s)	411
AciI	CCGC	5 cut(s)	422, 618, 1615, 1900, 1988
AclWI	GGATC	2 cut(s)	648, 670
AcoI	YGGCCR	2 cut(s)	190, 798
AcsI	RAATTY	2 cut(s)	1111, 1784
AcuI	CTGAAG	3 cut(s)	1320, 1794, 2154
AcyI	GRCGYC	2 cut(s)	1807, 1897
AdeI	CACNNNGTG	1 cut(s)	1745
AfaI	GTAC	7 cut(s)	791, 955, 1061, 1126, 1337, 1528, 2055
AfiI	CCNNNNNNNGG	6 cut(s)	199, 344, 765, 858, 1519, 1611
AflII	CTTAAG	1 cut(s)	1253
AflIII	ACRYGT	2 cut(s)	360, 1315
AhdI	GACNNNNNGTC	1 cut(s)	1555
AhlI	ACTAGT	1 cut(s)	148
AjiI	CACGTC	1 cut(s)	1316
AjnI	CCWGG	2 cut(s)	758, 1648
Alw21I	GWGCWC	1 cut(s)	2095
Alw26I	GTCTC	5 cut(s)	295, 1257, 1303, 1647, 1970
AlwI	GGATC	2 cut(s)	648, 670
AlwNI	CAGNNNCTG	1 cut(s)	770
Ama87I	CYCGRG	1 cut(s)	247
Aor13HI	TCCGGA	1 cut(s)	411
AoxI	GGCC	3 cut(s)	190, 798, 1171
ApeKI	GCWGC	8 cut(s)	770, 818, 1672, 1825, 1828, 1871, 1889, 2072
ApoI	RAATTY	2 cut(s)	1111, 1784
ArsI	GACNNNNNNTTYG	2 cut(s)	1302, 1334
Asp700I	GAANNNNTTC	1 cut(s)	1061
AspLEI	GCGC	5 cut(s)	478, 535, 632, 1019, 1899
AspS9I	GGNCC	2 cut(s)	517, 1171
AsuC2I	CCSGG	2 cut(s)	248, 249
AsuHPI	GGTGA	4 cut(s)	515, 1559, 1676, 1844
AsuII	TTCGAA	2 cut(s)	1011, 1441
AvaI	CYCGRG	1 cut(s)	247
AvaII	GGWCC	1 cut(s)	517
BalI	TGGCCA	2 cut(s)	192, 800
BanI	GGYRCC	1 cut(s)	1896
BanII	GRGCYC	1 cut(s)	2095
BauI	CACGAG	1 cut(s)	292
BbsI	GAAGAC	1 cut(s)	380
Bbv12I	GWGCWC	1 cut(s)	2095
BbvCI	CCTCAGC	1 cut(s)	536
BbvI	GCAGC	8 cut(s)	757, 830, 1659, 1812, 1840, 1858, 1876, 2084
BccI	CCATC	3 cut(s)	91, 1382, 1459
BceAI	ACGGC	1 cut(s)	65
BcgI	CGANNNNNNTGC	4 cut(s)	257, 291, 1820, 1854
BciT130I	CCWGG	2 cut(s)	760, 1650
BclI	TGATCA	1 cut(s)	1471
BcnI	CCSGG	2 cut(s)	248, 249
BcoDI	GTCTC	5 cut(s)	295, 1257, 1303, 1647, 1970
BcuI	ACTAGT	1 cut(s)	148
BfaI	CTAG	3 cut(s)	149, 914, 1353
BfmI	CTRYAG	1 cut(s)	1826
BfoI	RGCGCY	2 cut(s)	633, 1900
BfrI	CTTAAG	1 cut(s)	1253
BfuAI	ACCTGC	1 cut(s)	1399
BglII	AGATCT	4 cut(s)	1432, 1777, 1927, 1996
BisI	GCNGC	9 cut(s)	771, 819, 1673, 1826, 1829, 1872, 1890, 1900, 2073
BlsI	GCNGC	9 cut(s)	772, 820, 1674, 1827, 1830, 1873, 1891, 1901, 2074
BmcAI	AGTACT	3 cut(s)	1061, 1126, 2055
Bme1390I	CCNGG	4 cut(s)	248, 249, 760, 1650
Bme18I	GGWCC	1 cut(s)	517
BmeRI	GACNNNNNGTC	1 cut(s)	1555
BmeT110I	CYCGRG	1 cut(s)	247
BmgBI	CACGTC	1 cut(s)	1316
BmgT120I	GGNCC	2 cut(s)	517, 1171
BmiI	GGNNCC	2 cut(s)	519, 1898
BmrFI	CCNGG	4 cut(s)	248, 249, 760, 1650
BmrI	ACTGGG	1 cut(s)	1547
BmsI	GCATC	4 cut(s)	343, 1294, 1474, 2103
BmuI	ACTGGG	1 cut(s)	1547
BpiI	GAAGAC	1 cut(s)	380
BpmI	CTGGAG	2 cut(s)	759, 1671
Bpu10I	CCTNAGC	2 cut(s)	536, 1514
Bpu14I	TTCGAA	2 cut(s)	1011, 1441
BpuEI	CTTGAG	2 cut(s)	403, 1259
BpuMI	CCSGG	2 cut(s)	248, 249
BsaAI	YACGTR	1 cut(s)	363
BsaBI	GATNNNNATC	1 cut(s)	599
BsaHI	GRCGYC	2 cut(s)	1807, 1897
BsaJI	CCNNGG	3 cut(s)	224, 247, 759
BsaWI	WCCGGW	2 cut(s)	411, 2140
Bsc4I	CCNNNNNNNGG	6 cut(s)	199, 344, 765, 858, 1519, 1611
Bse1I	ACTGG	5 cut(s)	148, 742, 1553, 1624, 2061
Bse3DI	GCAATG	4 cut(s)	125, 275, 578, 1158
Bse8I	GATNNNNATC	1 cut(s)	599
BseAI	TCCGGA	1 cut(s)	411
BseBI	CCWGG	2 cut(s)	760, 1650
BseDI	CCNNGG	3 cut(s)	224, 247, 759
BseGI	GGATG	5 cut(s)	358, 1284, 1374, 1474, 1963
BseJI	GATNNNNATC	1 cut(s)	599
BseLI	CCNNNNNNNGG	6 cut(s)	199, 344, 765, 858, 1519, 1611
BseMI	GCAATG	4 cut(s)	125, 275, 578, 1158
BseMII	CTCAG	7 cut(s)	101, 527, 605, 1922, 1971, 2108, 2145
BseNI	ACTGG	5 cut(s)	148, 742, 1553, 1624, 2061
BseRI	GAGGAG	3 cut(s)	1256, 1371, 1682
BseXI	GCAGC	8 cut(s)	757, 830, 1659, 1812, 1840, 1858, 1876, 2084
BseYI	CCCAGC	1 cut(s)	343
BsgI	GTGCAG	1 cut(s)	1796
Bsh1236I	CGCG	1 cut(s)	489
BshFI	GGCC	3 cut(s)	192, 800, 1173
BshNI	GGYRCC	1 cut(s)	1896
BsiHKAI	GWGCWC	1 cut(s)	2095
BsiHKCI	CYCGRG	1 cut(s)	247
BsiSI	CCGG	3 cut(s)	248, 412, 2141
BslFI	GGGAC	3 cut(s)	671, 1433, 1597
BslI	CCNNNNNNNGG	6 cut(s)	199, 344, 765, 858, 1519, 1611
BsmAI	GTCTC	5 cut(s)	295, 1257, 1303, 1647, 1970
BsmBI	CGTCTC	1 cut(s)	1303
BsmFI	GGGAC	3 cut(s)	671, 1433, 1597
BsnI	GGCC	3 cut(s)	192, 800, 1173
BsoBI	CYCGRG	1 cut(s)	247
Bsp119I	TTCGAA	2 cut(s)	1011, 1441
Bsp1286I	GDGCHC	1 cut(s)	2095
Bsp13I	TCCGGA	1 cut(s)	411
Bsp1407I	TGTACA	1 cut(s)	789
BspACI	CCGC	5 cut(s)	422, 618, 1615, 1900, 1988
BspANI	GGCC	3 cut(s)	192, 800, 1173
BspCNI	CTCAG	7 cut(s)	100, 528, 604, 1923, 1972, 2107, 2146
BspEI	TCCGGA	1 cut(s)	411
BspFNI	CGCG	1 cut(s)	489
BspHI	TCATGA	2 cut(s)	217, 1053
BspLI	GGNNCC	2 cut(s)	519, 1898
BspMAI	CTGCAG	1 cut(s)	1830
BspMI	ACCTGC	1 cut(s)	1399
BspPI	GGATC	2 cut(s)	648, 670
BspQI	GCTCTTC	1 cut(s)	67
BspT104I	TTCGAA	2 cut(s)	1011, 1441
BspT107I	GGYRCC	1 cut(s)	1896
BspTI	CTTAAG	1 cut(s)	1253
BsrDI	GCAATG	4 cut(s)	125, 275, 578, 1158
BsrGI	TGTACA	1 cut(s)	789
BsrI	ACTGG	5 cut(s)	148, 742, 1553, 1624, 2061
BssECI	CCNNGG	3 cut(s)	224, 247, 759
BssNI	GRCGYC	2 cut(s)	1807, 1897
BssSI	CACGAG	1 cut(s)	292
BssT1I	CCWWGG	1 cut(s)	224
Bst2BI	CACGAG	1 cut(s)	292
Bst2UI	CCWGG	2 cut(s)	760, 1650
Bst4CI	ACNGT	3 cut(s)	1231, 1732, 2053
Bst6I	CTCTTC	4 cut(s)	67, 1255, 1438, 2026
BstACI	GRCGYC	2 cut(s)	1807, 1897
BstAFI	CTTAAG	1 cut(s)	1253
BstAPI	GCANNNNNTGC	1 cut(s)	770
BstAUI	TGTACA	1 cut(s)	789
BstBAI	YACGTR	1 cut(s)	363
BstBI	TTCGAA	2 cut(s)	1011, 1441
BstC8I	GCNNGC	8 cut(s)	798, 802, 1085, 1347, 1677, 1811, 1876, 1986
BstEII	GGTNACC	1 cut(s)	1547
BstF5I	GGATG	5 cut(s)	358, 1284, 1374, 1474, 1963
BstFNI	CGCG	1 cut(s)	489
BstH2I	RGCGCY	2 cut(s)	633, 1900
BstHHI	GCGC	5 cut(s)	478, 535, 632, 1019, 1899
BstMAI	GTCTC	5 cut(s)	295, 1257, 1303, 1647, 1970
BstMWI	GCNNNNNNNGC	5 cut(s)	473, 770, 1425, 1769, 1815
BstNI	CCWGG	2 cut(s)	760, 1650
BstNSI	RCATGY	2 cut(s)	446, 796
BstPI	GGTNACC	1 cut(s)	1547
BstSCI	CCNGG	4 cut(s)	246, 247, 758, 1648
BstSFI	CTRYAG	1 cut(s)	1826
BstUI	CGCG	1 cut(s)	489
BstV1I	GCAGC	8 cut(s)	757, 830, 1659, 1812, 1840, 1858, 1876, 2084
BstV2I	GAAGAC	1 cut(s)	380
BstX2I	RGATCY	4 cut(s)	1432, 1777, 1927, 1996
BstYI	RGATCY	4 cut(s)	1432, 1777, 1927, 1996
BsuRI	GGCC	3 cut(s)	192, 800, 1173
BtrI	CACGTC	1 cut(s)	1316
BtsCI	GGATG	5 cut(s)	358, 1284, 1374, 1474, 1963
BtsI	GCAGTG	1 cut(s)	1701
BtsIMutI	CAGTG	5 cut(s)	141, 1227, 1560, 1701, 2151
BveI	ACCTGC	1 cut(s)	1399
Cac8I	GCNNGC	8 cut(s)	798, 802, 1085, 1347, 1677, 1811, 1876, 1986
CaiI	CAGNNNCTG	1 cut(s)	770
CciI	TCATGA	2 cut(s)	217, 1053
CfoI	GCGC	5 cut(s)	478, 535, 632, 1019, 1899
Cfr13I	GGNCC	2 cut(s)	517, 1171
Cfr9I	CCCGGG	1 cut(s)	247
CseI	GACGC	2 cut(s)	1332, 1815
Csp6I	GTAC	7 cut(s)	790, 954, 1060, 1125, 1336, 1527, 2054
CspCI	CAANNNNNGTGG	2 cut(s)	723, 758
CviQI	GTAC	7 cut(s)	790, 954, 1060, 1125, 1336, 1527, 2054
DinI	GGCGCC	1 cut(s)	1898
DraIII	CACNNNGTG	1 cut(s)	1745
DriI	GACNNNNNGTC	1 cut(s)	1555
EaeI	YGGCCR	2 cut(s)	190, 798
Eam1104I	CTCTTC	4 cut(s)	67, 1255, 1438, 2026
Eam1105I	GACNNNNNGTC	1 cut(s)	1555
EarI	CTCTTC	4 cut(s)	67, 1255, 1438, 2026
EciI	GGCGGA	1 cut(s)	1630
Ecl136II	GAGCTC	1 cut(s)	2093
Eco130I	CCWWGG	1 cut(s)	224
Eco24I	GRGCYC	1 cut(s)	2095
Eco32I	GATATC	1 cut(s)	427
Eco47I	GGWCC	1 cut(s)	517
Eco53kI	GAGCTC	1 cut(s)	2093
Eco57I	CTGAAG	3 cut(s)	1320, 1794, 2154
Eco88I	CYCGRG	1 cut(s)	247
Eco91I	GGTNACC	1 cut(s)	1547
EcoICRI	GAGCTC	1 cut(s)	2093
EcoO65I	GGTNACC	1 cut(s)	1547
EcoRII	CCWGG	2 cut(s)	758, 1648
EcoRV	GATATC	1 cut(s)	427
EcoT14I	CCWWGG	1 cut(s)	224
EcoT38I	GRGCYC	1 cut(s)	2095
EgeI	GGCGCC	1 cut(s)	1898
EheI	GGCGCC	1 cut(s)	1898
ErhI	CCWWGG	1 cut(s)	224
Esp3I	CGTCTC	1 cut(s)	1303
FalI	AAGNNNNNCTT	6 cut(s)	365, 397, 1435, 1467, 1983, 2015
FaqI	GGGAC	3 cut(s)	671, 1433, 1597
FauI	CCCGC	1 cut(s)	625
FbaI	TGATCA	1 cut(s)	1471
Fnu4HI	GCNGC	9 cut(s)	771, 819, 1673, 1826, 1829, 1872, 1890, 1900, 2073
FokI	GGATG	5 cut(s)	365, 1291, 1361, 1481, 1970
FriOI	GRGCYC	1 cut(s)	2095
Fsp4HI	GCNGC	9 cut(s)	771, 819, 1673, 1826, 1829, 1872, 1890, 1900, 2073
FspBI	CTAG	3 cut(s)	149, 914, 1353
GlaI	GCGC	5 cut(s)	477, 534, 631, 1018, 1898
GluI	GCNGC	9 cut(s)	771, 819, 1673, 1826, 1829, 1872, 1890, 1900, 2073
GsaI	CCCAGC	1 cut(s)	347
GsuI	CTGGAG	2 cut(s)	759, 1671
HaeII	RGCGCY	2 cut(s)	633, 1900
HaeIII	GGCC	3 cut(s)	192, 800, 1173
HapII	CCGG	3 cut(s)	248, 412, 2141
HgaI	GACGC	2 cut(s)	1332, 1815
HhaI	GCGC	5 cut(s)	478, 535, 632, 1019, 1899
Hin1I	GRCGYC	2 cut(s)	1807, 1897
Hin6I	GCGC	5 cut(s)	476, 533, 630, 1017, 1897
HinP1I	GCGC	5 cut(s)	476, 533, 630, 1017, 1897
HincII	GTYRAC	3 cut(s)	316, 1119, 1292
HindII	GTYRAC	3 cut(s)	316, 1119, 1292
HindIII	AAGCTT	1 cut(s)	1755
HinfI	GANTC	8 cut(s)	59, 274, 1370, 1446, 1585, 1623, 1943, 2038
HpaII	CCGG	3 cut(s)	248, 412, 2141
HphI	GGTGA	4 cut(s)	515, 1559, 1676, 1844
Hpy166II	GTNNAC	4 cut(s)	316, 506, 1119, 1292
Hpy8I	GTNNAC	4 cut(s)	316, 506, 1119, 1292
Hpy99I	CGWCG	3 cut(s)	1317, 1326, 1407
HpyAV	CCTTC	4 cut(s)	7, 1388, 1725, 2079
HpyCH4III	ACNGT	3 cut(s)	1231, 1732, 2053
HpyCH4IV	ACGT	3 cut(s)	362, 1142, 1315
HpyF10VI	GCNNNNNNNGC	5 cut(s)	473, 770, 1425, 1769, 1815
HpySE526I	ACGT	3 cut(s)	362, 1142, 1315
Hsp92I	GRCGYC	2 cut(s)	1807, 1897
HspAI	GCGC	5 cut(s)	476, 533, 630, 1017, 1897
KasI	GGCGCC	1 cut(s)	1896
Kpn2I	TCCGGA	1 cut(s)	411
Ksp22I	TGATCA	1 cut(s)	1471
LguI	GCTCTTC	1 cut(s)	67
LmnI	GCTCC	3 cut(s)	1669, 1695, 2090
Lsp1109I	GCAGC	8 cut(s)	757, 830, 1659, 1812, 1840, 1858, 1876, 2084
LweI	GCATC	4 cut(s)	343, 1294, 1474, 2103
MaeI	CTAG	3 cut(s)	149, 914, 1353
MaeII	ACGT	3 cut(s)	362, 1142, 1315
MaeIII	GTNAC	6 cut(s)	400, 745, 920, 1021, 1547, 2047
MfeI	CAATTG	1 cut(s)	837
MflI	RGATCY	4 cut(s)	1432, 1777, 1927, 1996
MhlI	GDGCHC	1 cut(s)	2095
MlsI	TGGCCA	2 cut(s)	192, 800
MluNI	TGGCCA	2 cut(s)	192, 800
Mly113I	GGCGCC	1 cut(s)	1897
MlyI	GAGTC	2 cut(s)	1455, 1579
Mox20I	TGGCCA	2 cut(s)	192, 800
MroI	TCCGGA	1 cut(s)	411
MroXI	GAANNNNTTC	1 cut(s)	1061
MscI	TGGCCA	2 cut(s)	192, 800
MseI	TTAA	6 cut(s)	132, 713, 1193, 1254, 1643, 1734
Msp20I	TGGCCA	2 cut(s)	192, 800
MspCI	CTTAAG	1 cut(s)	1253
MspI	CCGG	3 cut(s)	248, 412, 2141
MspR9I	CCNGG	4 cut(s)	248, 249, 760, 1650
MunI	CAATTG	1 cut(s)	837
MvaI	CCWGG	2 cut(s)	760, 1650
MvnI	CGCG	1 cut(s)	489
MwoI	GCNNNNNNNGC	5 cut(s)	473, 770, 1425, 1769, 1815
NarI	GGCGCC	1 cut(s)	1897
NciI	CCSGG	2 cut(s)	248, 249
NlaIV	GGNNCC	2 cut(s)	519, 1898
NmuCI	GTSAC	5 cut(s)	400, 745, 920, 1547, 2047
NspI	RCATGY	2 cut(s)	446, 796
NspV	TTCGAA	2 cut(s)	1011, 1441
PagI	TCATGA	2 cut(s)	217, 1053
PciSI	GCTCTTC	1 cut(s)	67
PcsI	WCGNNNNNNNCGW	1 cut(s)	1318
PdmI	GAANNNNTTC	1 cut(s)	1061
PfeI	GAWTC	6 cut(s)	59, 274, 1370, 1623, 1943, 2038
PkrI	GCNGC	9 cut(s)	772, 820, 1674, 1827, 1830, 1873, 1891, 1901, 2074
PleI	GAGTC	2 cut(s)	1454, 1579
PluTI	GGCGCC	1 cut(s)	1900
PpsI	GAGTC	2 cut(s)	1454, 1579
Ppu21I	YACGTR	1 cut(s)	363
PsiI	TTATAA	1 cut(s)	1577
Psp124BI	GAGCTC	1 cut(s)	2095
Psp6I	CCWGG	2 cut(s)	758, 1648
PspEI	GGTNACC	1 cut(s)	1547
PspFI	CCCAGC	1 cut(s)	343
PspGI	CCWGG	2 cut(s)	758, 1648
PspN4I	GGNNCC	2 cut(s)	519, 1898
PspPI	GGNCC	2 cut(s)	517, 1171
PstI	CTGCAG	1 cut(s)	1830
PstNI	CAGNNNCTG	1 cut(s)	770
PsuI	RGATCY	4 cut(s)	1432, 1777, 1927, 1996
RsaI	GTAC	7 cut(s)	791, 955, 1061, 1126, 1337, 1528, 2055
RsaNI	GTAC	7 cut(s)	790, 954, 1060, 1125, 1336, 1527, 2054
SacI	GAGCTC	1 cut(s)	2095
SapI	GCTCTTC	1 cut(s)	67
SaqAI	TTAA	6 cut(s)	132, 713, 1193, 1254, 1643, 1734
SatI	GCNGC	9 cut(s)	771, 819, 1673, 1826, 1829, 1872, 1890, 1900, 2073
Sau96I	GGNCC	2 cut(s)	517, 1171
ScaI	AGTACT	3 cut(s)	1061, 1126, 2055
SchI	GAGTC	2 cut(s)	1455, 1579
ScrFI	CCNGG	4 cut(s)	248, 249, 760, 1650
SduI	GDGCHC	1 cut(s)	2095
SfaNI	GCATC	4 cut(s)	343, 1294, 1474, 2103
SfcI	CTRYAG	1 cut(s)	1826
SfoI	GGCGCC	1 cut(s)	1898
SfuI	TTCGAA	2 cut(s)	1011, 1441
SinI	GGWCC	1 cut(s)	517
SmaI	CCCGGG	1 cut(s)	249
SmlI	CTYRAG	3 cut(s)	382, 1238, 1253
SmoI	CTYRAG	3 cut(s)	382, 1238, 1253
SpeI	ACTAGT	1 cut(s)	148
SsiI	CCGC	5 cut(s)	422, 618, 1615, 1900, 1988
SspDI	GGCGCC	1 cut(s)	1896
SspMI	CTAG	3 cut(s)	149, 914, 1353
SstI	GAGCTC	1 cut(s)	2095
StyD4I	CCNGG	4 cut(s)	246, 247, 758, 1648
StyI	CCWWGG	1 cut(s)	224
TaaI	ACNGT	3 cut(s)	1231, 1732, 2053
TaiI	ACGT	3 cut(s)	365, 1145, 1318
TaqI	TCGA	4 cut(s)	1011, 1321, 1441, 1482
TaqII	GACCGA	1 cut(s)	858
TatI	WGTACW	6 cut(s)	789, 953, 1059, 1124, 1335, 2053
TauI	GCSGC	1 cut(s)	1902
TfiI	GAWTC	6 cut(s)	59, 274, 1370, 1623, 1943, 2038
Tru1I	TTAA	6 cut(s)	132, 713, 1193, 1254, 1643, 1734
Tru9I	TTAA	6 cut(s)	132, 713, 1193, 1254, 1643, 1734
TscAI	CASTG	5 cut(s)	148, 1234, 1560, 1708, 2158
TseFI	GTSAC	5 cut(s)	400, 745, 920, 1547, 2047
TseI	GCWGC	8 cut(s)	770, 818, 1672, 1825, 1828, 1871, 1889, 2072
Tsp45I	GTSAC	5 cut(s)	400, 745, 920, 1547, 2047
TspGWI	ACGGA	1 cut(s)	653
TspMI	CCCGGG	1 cut(s)	247
TspRI	CASTG	5 cut(s)	148, 1234, 1560, 1708, 2158
Vha464I	CTTAAG	1 cut(s)	1253
VpaK11BI	GGWCC	1 cut(s)	517
XapI	RAATTY	2 cut(s)	1111, 1784
XbaI	TCTAGA	1 cut(s)	913
XceI	RCATGY	2 cut(s)	446, 796
XmaI	CCCGGG	1 cut(s)	247
XmnI	GAANNNNTTC	1 cut(s)	1061
XspI	CTAG	3 cut(s)	149, 914, 1353
ZrmI	AGTACT	3 cut(s)	1061, 1126, 2055

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.