Rh4BG117800

cyclic nucleotide-gated ion channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0008944
Physical Location & Seq
Reverse (-)
19802883 .. 19805675
2793 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG117800.1

Sequence Viewer

Length: 1137 bp
ATGGAATTGAAGAAGGAGAGGCTTGTCAGGTTTTATTCTGATAAAAAACACCATAGAGATTCTATATGGGACAGAACTGAACCTTATCGTCTCGAAAAGTCGGCCTCTGCATATAGGGTTGCATCATCTTCGTTGCTCAAATCTGATAATGGACTAGCGGGGGGCACACGCAGATTTGCCGAAAATTTCAGAATCAGGCATTCAAAGGTGTTTCAAGAGGACCATGAGCCATGGCGCAAGAGAATACTTGACCCAGGTAGTGAAATAGTGCTGCAATGGAATCGAGTTTTCATTGTCTCGTGCTTGGTTGCACTTTTTGTGGACCCATTGTACTTTTACTTGCCCATGGCTGAAGGGAGTGTAAACAGTTTTTGCATGAAGACAGACTTGAATTTGCGAATTATTGTGACTTTTTTCCGGACTATTGCGGATTTCTTCTATTTTTTGCACATGATTATAAAGTTTAGGACGGCTTATGTTGCACCAAGCTCCCGCGTTTTCGGGAGGGGTGAACTTGTCATGGACCCAAAGAAGATTGCACAGAGATACATTACATCCGACTTCTCCATTGATCTCATTGCCACATTGCCACTTCCTCAGATAGTCATCTGGTTTATCATACCTCCCCAAAATGCTCAAACGGATAACACCAACAATGCACTGGCTCTAATTGTTCTCCTCCAGTACATCCCCAGATTATATCTGATTTTCCCTTTAAGTTTGCAATTAATCAAAGCAACTGGAGTGGTGACAAAAACTGCTTGGGCAGGGGCTGCATATAATCTGCTATTATACATGCTGGCCAGCCATGTTTTAGGGGCAGCATGGTATTTGCTATCTATTGACCGGTATGCCTCATGCTGGAAAACATATTGTAAAAAGGATCTCAGCCCCGTAAAATGCTTGCCTAACTATTTAGATTGTAATACTGTTGACCAAGTTCTTCGCAAGACATGGGCAAGTAGTACAAATGTTTTTAAAAAATGCGATCCTGAAAGTAACATATTCAAGTATGGAATATTTGGAACTGCACTCACAAAAAATGCTGTTTCGTCAAACTTCATCACAAAGTACTTCTATTGCTTATGGTGGGGTTTACAGCAACTAAGGTATGGGGCCTTTTCTTTTCTAAACTAG

Protein Analysis

378

Amino Acids

43.77

Weight (kDa)

9.51

Isoelectric Point (pI)

32.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 93 - 367 7.2e-13 Ion transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 458
AccII CGCG 1 cut(s) 495
AccIII TCCGGA 1 cut(s) 417
AciI CCGC 3 cut(s) 158, 428, 493
AclWI GGATC 2 cut(s) 891, 983
AcoI YGGCCR 1 cut(s) 801
AcsI RAATTY 2 cut(s) 184, 391
AcuI CTGAAG 1 cut(s) 372
AfaI GTAC 4 cut(s) 332, 686, 967, 1073
AfiI CCNNNNNNNGG 1 cut(s) 861
AgeI ACCGGT 1 cut(s) 846
AgsI TTSAA 5 cut(s) 10, 204, 215, 391, 1009
AjnI CCWGG 1 cut(s) 253
AluBI AGCT 1 cut(s) 489
AluI AGCT 1 cut(s) 489
Alw26I GTCTC 2 cut(s) 95, 301
AlwI GGATC 2 cut(s) 891, 983
AlwNI CAGNNNCTG 1 cut(s) 773
Aor13HI TCCGGA 1 cut(s) 417
AoxI GGCC 3 cut(s) 102, 801, 1116
ApeKI GCWGC 3 cut(s) 271, 773, 821
ApoI RAATTY 2 cut(s) 184, 391
AseI ATTAAT 1 cut(s) 728
AsiGI ACCGGT 1 cut(s) 846
AspLEI GCGC 1 cut(s) 237
AspS9I GGNCC 4 cut(s) 220, 322, 523, 1116
AsuHPI GGTGA 2 cut(s) 521, 760
AvaII GGWCC 3 cut(s) 220, 322, 523
BaeGI GKGCMC 1 cut(s) 167
BalI TGGCCA 1 cut(s) 803
BauI CACGAG 1 cut(s) 298
BbsI GAAGAC 1 cut(s) 386
BbvI GCAGC 3 cut(s) 258, 760, 833
BceAI ACGGC 1 cut(s) 486
BcgI CGANNNNNNTGC 4 cut(s) 111, 145, 263, 297
BciT130I CCWGG 1 cut(s) 255
BcoDI GTCTC 2 cut(s) 95, 301
BfaI CTAG 2 cut(s) 155, 1135
BisI GCNGC 3 cut(s) 272, 774, 822
BlsI GCNGC 3 cut(s) 273, 775, 823
BmcAI AGTACT 1 cut(s) 1073
Bme1390I CCNGG 1 cut(s) 255
Bme18I GGWCC 3 cut(s) 220, 322, 523
BmgT120I GGNCC 4 cut(s) 220, 322, 523, 1116
BmiI GGNNCC 3 cut(s) 324, 525, 1117
BmrFI CCNGG 1 cut(s) 255
BmsI GCATC 1 cut(s) 131
BpiI GAAGAC 1 cut(s) 386
BpmI CTGGAG 2 cut(s) 665, 762
BsaBI GATNNNNATC 1 cut(s) 605
BsaJI CCNNGG 3 cut(s) 230, 253, 345
BsaWI WCCGGW 2 cut(s) 417, 846
Bsc4I CCNNNNNNNGG 1 cut(s) 861
Bse118I RCCGGY 1 cut(s) 846
Bse1I ACTGG 3 cut(s) 666, 682, 745
Bse3DI GCAATG 3 cut(s) 281, 576, 584
Bse8I GATNNNNATC 1 cut(s) 605
BseAI TCCGGA 1 cut(s) 417
BseBI CCWGG 1 cut(s) 255
BseDI CCNNGG 3 cut(s) 230, 253, 345
BseGI GGATG 2 cut(s) 554, 687
BseJI GATNNNNATC 1 cut(s) 605
BseLI CCNNNNNNNGG 1 cut(s) 861
BseMI GCAATG 3 cut(s) 281, 576, 584
BseMII CTCAG 2 cut(s) 611, 901
BseNI ACTGG 3 cut(s) 666, 682, 745
BseRI GAGGAG 1 cut(s) 668
BseSI GKGCMC 1 cut(s) 167
BseXI GCAGC 3 cut(s) 258, 760, 833
BsgI GTGCAG 1 cut(s) 1014
Bsh1236I CGCG 1 cut(s) 495
BshFI GGCC 3 cut(s) 104, 803, 1118
BshTI ACCGGT 1 cut(s) 846
BsiSI CCGG 2 cut(s) 418, 847
BslFI GGGAC 1 cut(s) 83
BslI CCNNNNNNNGG 1 cut(s) 861
BsmAI GTCTC 2 cut(s) 95, 301
BsmBI CGTCTC 1 cut(s) 95
BsmFI GGGAC 1 cut(s) 83
BsmI GAATGC 1 cut(s) 199
BsnI GGCC 3 cut(s) 104, 803, 1118
Bsp1286I GDGCHC 1 cut(s) 167
Bsp13I TCCGGA 1 cut(s) 417
Bsp143I GATC 3 cut(s) 571, 883, 988
Bsp19I CCATGG 2 cut(s) 230, 345
BspACI CCGC 3 cut(s) 158, 428, 493
BspANI GGCC 3 cut(s) 104, 803, 1118
BspCNI CTCAG 2 cut(s) 610, 900
BspEI TCCGGA 1 cut(s) 417
BspFNI CGCG 1 cut(s) 495
BspLI GGNNCC 3 cut(s) 324, 525, 1117
BspPI GGATC 2 cut(s) 891, 983
BsrDI GCAATG 3 cut(s) 281, 576, 584
BsrFI RCCGGY 1 cut(s) 846
BsrI ACTGG 3 cut(s) 666, 682, 745
BssAI RCCGGY 1 cut(s) 846
BssECI CCNNGG 3 cut(s) 230, 253, 345
BssMI GATC 3 cut(s) 571, 883, 988
BssSI CACGAG 1 cut(s) 298
BssT1I CCWWGG 2 cut(s) 230, 345
Bst2BI CACGAG 1 cut(s) 298
Bst2UI CCWGG 1 cut(s) 255
Bst4CI ACNGT 2 cut(s) 368, 931
BstAPI GCANNNNNTGC 1 cut(s) 773
BstC8I GCNNGC 3 cut(s) 801, 805, 905
BstDEI CTNAG 3 cut(s) 597, 887, 1106
BstDSI CCRYGG 2 cut(s) 230, 345
BstF5I GGATG 2 cut(s) 554, 687
BstFNI CGCG 1 cut(s) 495
BstHHI GCGC 1 cut(s) 237
BstKTI GATC 3 cut(s) 574, 886, 991
BstMAI GTCTC 2 cut(s) 95, 301
BstMBI GATC 3 cut(s) 571, 883, 988
BstMWI GCNNNNNNNGC 2 cut(s) 479, 773
BstNI CCWGG 1 cut(s) 255
BstNSI RCATGY 1 cut(s) 799
BstSCI CCNGG 1 cut(s) 253
BstSLI GKGCMC 1 cut(s) 167
BstUI CGCG 1 cut(s) 495
BstV1I GCAGC 3 cut(s) 258, 760, 833
BstV2I GAAGAC 1 cut(s) 386
BstX2I RGATCY 1 cut(s) 883
BstYI RGATCY 1 cut(s) 883
BsuRI GGCC 3 cut(s) 104, 803, 1118
BtgI CCRYGG 2 cut(s) 230, 345
BtsCI GGATG 2 cut(s) 554, 687
BtsIMutI CAGTG 1 cut(s) 659
Cac8I GCNNGC 3 cut(s) 801, 805, 905
CaiI CAGNNNCTG 1 cut(s) 773
CfoI GCGC 1 cut(s) 237
Cfr10I RCCGGY 1 cut(s) 846
Cfr13I GGNCC 4 cut(s) 220, 322, 523, 1116
Csp6I GTAC 4 cut(s) 331, 685, 966, 1072
CspAI ACCGGT 1 cut(s) 846
CspCI CAANNNNNGTGG 2 cut(s) 726, 761
CviQI GTAC 4 cut(s) 331, 685, 966, 1072
DdeI CTNAG 3 cut(s) 597, 887, 1106
DpnI GATC 3 cut(s) 573, 885, 990
DpnII GATC 3 cut(s) 571, 883, 988
DraI TTTAAA 1 cut(s) 979
EaeI YGGCCR 1 cut(s) 801
Eco130I CCWWGG 2 cut(s) 230, 345
Eco47I GGWCC 3 cut(s) 220, 322, 523
Eco57I CTGAAG 1 cut(s) 372
EcoO109I RGGNCCY 1 cut(s) 1116
EcoRII CCWGG 1 cut(s) 253
EcoT14I CCWWGG 2 cut(s) 230, 345
ErhI CCWWGG 2 cut(s) 230, 345
Esp3I CGTCTC 1 cut(s) 95
FalI AAGNNNNNCTT 2 cut(s) 371, 403
FaqI GGGAC 1 cut(s) 83
FauI CCCGC 2 cut(s) 151, 500
Fnu4HI GCNGC 3 cut(s) 272, 774, 822
FokI GGATG 2 cut(s) 541, 674
Fsp4HI GCNGC 3 cut(s) 272, 774, 822
FspBI CTAG 2 cut(s) 155, 1135
GlaI GCGC 1 cut(s) 236
GluI GCNGC 3 cut(s) 272, 774, 822
GsuI CTGGAG 2 cut(s) 665, 762
HaeIII GGCC 3 cut(s) 104, 803, 1118
HapII CCGG 2 cut(s) 418, 847
HhaI GCGC 1 cut(s) 237
Hin6I GCGC 1 cut(s) 235
HinP1I GCGC 1 cut(s) 235
HincII GTYRAC 1 cut(s) 934
HindII GTYRAC 1 cut(s) 934
HinfI GANTC 3 cut(s) 59, 192, 280
HpaII CCGG 2 cut(s) 418, 847
HphI GGTGA 2 cut(s) 521, 760
Hpy166II GTNNAC 5 cut(s) 322, 364, 512, 934, 1097
Hpy188I TCNGA 6 cut(s) 40, 145, 191, 559, 600, 705
Hpy188III TCNNGA 5 cut(s) 92, 215, 418, 502, 992
Hpy8I GTNNAC 5 cut(s) 322, 364, 512, 934, 1097
HpyAV CCTTC 2 cut(s) 7, 347
HpyCH4III ACNGT 2 cut(s) 368, 931
HpyF10VI GCNNNNNNNGC 2 cut(s) 479, 773
HpyF3I CTNAG 3 cut(s) 597, 887, 1106
HspAI GCGC 1 cut(s) 235
Kpn2I TCCGGA 1 cut(s) 417
Kzo9I GATC 3 cut(s) 571, 883, 988
LmnI GCTCC 1 cut(s) 494
Lsp1109I GCAGC 3 cut(s) 258, 760, 833
LweI GCATC 1 cut(s) 131
MaeI CTAG 2 cut(s) 155, 1135
MaeIII GTNAC 3 cut(s) 406, 748, 998
MalI GATC 3 cut(s) 573, 885, 990
MboI GATC 3 cut(s) 571, 883, 988
MboII GAAGA 6 cut(s) 22, 120, 391, 427, 544, 935
MflI RGATCY 1 cut(s) 883
MhlI GDGCHC 1 cut(s) 167
MlsI TGGCCA 1 cut(s) 803
MluCI AATT 6 cut(s) 5, 184, 391, 399, 669, 725
MluNI TGGCCA 1 cut(s) 803
MmeI TCCRAC 1 cut(s) 582
MnlI CCTC 8 cut(s) 12, 115, 211, 498, 606, 633, 689, 865
Mox20I TGGCCA 1 cut(s) 803
MroI TCCGGA 1 cut(s) 417
MscI TGGCCA 1 cut(s) 803
MseI TTAA 3 cut(s) 716, 728, 978
Msp20I TGGCCA 1 cut(s) 803
MspI CCGG 2 cut(s) 418, 847
MspR9I CCNGG 1 cut(s) 255
Mva1269I GAATGC 1 cut(s) 199
MvaI CCWGG 1 cut(s) 255
MvnI CGCG 1 cut(s) 495
MwoI GCNNNNNNNGC 2 cut(s) 479, 773
NcoI CCATGG 2 cut(s) 230, 345
NdeII GATC 3 cut(s) 571, 883, 988
NlaIV GGNNCC 3 cut(s) 324, 525, 1117
NmuCI GTSAC 2 cut(s) 406, 748
NspI RCATGY 1 cut(s) 799
PctI GAATGC 1 cut(s) 199
PfeI GAWTC 3 cut(s) 59, 192, 280
PinAI ACCGGT 1 cut(s) 846
PkrI GCNGC 3 cut(s) 273, 775, 823
PshBI ATTAAT 1 cut(s) 728
PsiI TTATAA 1 cut(s) 458
Psp6I CCWGG 1 cut(s) 253
PspGI CCWGG 1 cut(s) 253
PspN4I GGNNCC 3 cut(s) 324, 525, 1117
PspPI GGNCC 4 cut(s) 220, 322, 523, 1116
PstNI CAGNNNCTG 1 cut(s) 773
PsuI RGATCY 1 cut(s) 883
RsaI GTAC 4 cut(s) 332, 686, 967, 1073
RsaNI GTAC 4 cut(s) 331, 685, 966, 1072
SaqAI TTAA 3 cut(s) 716, 728, 978
SatI GCNGC 3 cut(s) 272, 774, 822
Sau3AI GATC 3 cut(s) 571, 883, 988
Sau96I GGNCC 4 cut(s) 220, 322, 523, 1116
ScaI AGTACT 1 cut(s) 1073
ScrFI CCNGG 1 cut(s) 255
SduI GDGCHC 1 cut(s) 167
SetI ASST 7 cut(s) 32, 85, 210, 259, 491, 625, 1112
SfaNI GCATC 1 cut(s) 131
SinI GGWCC 3 cut(s) 220, 322, 523
Sse9I AATT 6 cut(s) 5, 184, 391, 399, 669, 725
SsiI CCGC 3 cut(s) 158, 428, 493
SspI AATATT 1 cut(s) 1020
SspMI CTAG 2 cut(s) 155, 1135
StyD4I CCNGG 1 cut(s) 253
StyI CCWWGG 2 cut(s) 230, 345
TaaI ACNGT 2 cut(s) 368, 931
TaqI TCGA 2 cut(s) 93, 283
TasI AATT 6 cut(s) 5, 184, 391, 399, 669, 725
TatI WGTACW 4 cut(s) 330, 684, 965, 1071
TfiI GAWTC 3 cut(s) 59, 192, 280
Tru1I TTAA 3 cut(s) 716, 728, 978
Tru9I TTAA 3 cut(s) 716, 728, 978
TscAI CASTG 1 cut(s) 666
TseFI GTSAC 2 cut(s) 406, 748
TseI GCWGC 3 cut(s) 271, 773, 821
Tsp45I GTSAC 2 cut(s) 406, 748
TspDTI ATGAA 3 cut(s) 280, 392, 1051
TspGWI ACGGA 1 cut(s) 656
TspRI CASTG 1 cut(s) 666
VpaK11BI GGWCC 3 cut(s) 220, 322, 523
VspI ATTAAT 1 cut(s) 728
XapI RAATTY 2 cut(s) 184, 391
XceI RCATGY 1 cut(s) 799
XcmI CCANNNNNNNNNTGG 1 cut(s) 658
XspI CTAG 2 cut(s) 155, 1135
ZrmI AGTACT 1 cut(s) 1073
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.