Rroxscaffold_5G00348770

cyclic nucleotide-gated ion channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0008944
Physical Location & Seq
Reverse (-)
21251514 .. 21257434
5921 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00348770.1

Sequence Viewer

Length: 714 bp
ATGGATGATCAGCTTCTTGATGCAATATGTGAACGTCTTGTTTCCTCCCTAAGCACAGCAGGCACTTATATTGTCAGAGAGGGTGATCCAGTGACAGAAATGCTTTTCATTATTAGAGGTACTCTCGATAGTTCTACTACCAATGGAGGCCGGACTGGTTTCTTCAATTCAATAACATTAAGACCTGGGGACTTCTGCGGTGAGGAGCTACTTGCATGGGCGCTGCTCCCAAAATCCACTGTCAACATGCCTTCTTCAACAAGAACAGTAATAGCACTTTCTGAAGTTGAAGCTTTTGCACTGCGAGCTGAAGATCTGAAATTTGTGGCCAACCAATTTAGACGCCTGCACAGCAAGAAGCTGCAGCACACCTTTCGGTTTTACTCTCACCATTGGAGAACATGGGCGGCCTGCTTCATACAGGCTGCTTGGCGTCGCCACAAGAGAAGGATGATGGCAAAAGATCTTAGCTTGAGGGAGTCCTTTGCTTTGGATGAGAAAGTAGCCTATGAGACAGCACAAGAGGAGGAAGAAGAAGAAGAACATCATTCTGCTGGTTCAAATTCTTCGCAAGCAAAAGTAAATCTTGGGGTCACACTACTGGCCTCAAGGTTTGCTGCAAACACAAGGAGGGGTGCTCAGAAGATCAAAGACGATATGCCCAGGTTACCAAAACCTGCAGAACCTGACTTCTCCACTGAGCCTGAATATTAG

Protein Analysis

237

Amino Acids

26.87

Weight (kDa)

6.4

Isoelectric Point (pI)

49.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
cNMP_binding PF00027 20 - 109 1.3e-09 Cyclic nucleotide-binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 685
AciI CCGC 2 cut(s) 198, 407
AclWI GGATC 1 cut(s) 80
AcoI YGGCCR 1 cut(s) 327
AcsI RAATTY 2 cut(s) 320, 562
AcuI CTGAAG 2 cut(s) 303, 330
AcyI GRCGYC 2 cut(s) 343, 433
AfaI GTAC 1 cut(s) 121
AgsI TTSAA 5 cut(s) 166, 171, 258, 290, 561
AjnI CCWGG 2 cut(s) 184, 662
AluBI AGCT 6 cut(s) 13, 208, 293, 308, 361, 471
AluI AGCT 6 cut(s) 13, 208, 293, 308, 361, 471
Alw21I GWGCWC 1 cut(s) 640
Alw26I GTCTC 1 cut(s) 506
AlwI GGATC 1 cut(s) 80
AlwNI CAGNNNCTG 1 cut(s) 686
AoxI GGCC 4 cut(s) 148, 327, 408, 603
ApeKI GCWGC 5 cut(s) 223, 361, 364, 425, 617
ApoI RAATTY 2 cut(s) 320, 562
AspLEI GCGC 1 cut(s) 223
AsuHPI GGTGA 3 cut(s) 95, 212, 380
BalI TGGCCA 1 cut(s) 329
Bbv12I GWGCWC 1 cut(s) 640
BbvI GCAGC 5 cut(s) 210, 348, 376, 412, 604
BccI CCATC 1 cut(s) 448
BcgI CGANNNNNNTGC 2 cut(s) 356, 390
BciT130I CCWGG 2 cut(s) 186, 664
BclI TGATCA 1 cut(s) 7
BcoDI GTCTC 1 cut(s) 506
BfmI CTRYAG 2 cut(s) 362, 678
BfoI RGCGCY 1 cut(s) 224
BfuAI ACCTGC 1 cut(s) 685
BglII AGATCT 2 cut(s) 313, 463
BisI GCNGC 6 cut(s) 224, 362, 365, 408, 426, 618
BlsI GCNGC 6 cut(s) 225, 363, 366, 409, 427, 619
Bme1390I CCNGG 2 cut(s) 186, 664
BmrFI CCNGG 2 cut(s) 186, 664
BmsI GCATC 1 cut(s) 10
BplI GAGNNNNNCTC 4 cut(s) 108, 140, 622, 654
Bpu10I CCTNAGC 1 cut(s) 50
BpuEI CTTGAG 2 cut(s) 493, 592
BsaHI GRCGYC 2 cut(s) 343, 433
BsaJI CCNNGG 2 cut(s) 185, 662
Bse1I ACTGG 3 cut(s) 89, 160, 606
BseBI CCWGG 2 cut(s) 186, 664
BseDI CCNNGG 2 cut(s) 185, 662
BseGI GGATG 3 cut(s) 10, 456, 499
BseMII CTCAG 2 cut(s) 653, 690
BseNI ACTGG 3 cut(s) 89, 160, 606
BseRI GAGGAG 2 cut(s) 218, 539
BseXI GCAGC 5 cut(s) 210, 348, 376, 412, 604
BsgI GTGCAG 1 cut(s) 332
BshFI GGCC 4 cut(s) 150, 329, 410, 605
BsiHKAI GWGCWC 1 cut(s) 640
BsiSI CCGG 1 cut(s) 151
BslFI GGGAC 1 cut(s) 203
BsmAI GTCTC 1 cut(s) 506
BsmFI GGGAC 1 cut(s) 203
BsnI GGCC 4 cut(s) 150, 329, 410, 605
Bsp1286I GDGCHC 1 cut(s) 640
Bsp143I GATC 5 cut(s) 7, 85, 313, 463, 645
BspACI CCGC 2 cut(s) 198, 407
BspANI GGCC 4 cut(s) 150, 329, 410, 605
BspCNI CTCAG 2 cut(s) 652, 691
BspMAI CTGCAG 2 cut(s) 366, 682
BspMI ACCTGC 1 cut(s) 685
BspPI GGATC 1 cut(s) 80
BsrI ACTGG 3 cut(s) 89, 160, 606
BssECI CCNNGG 2 cut(s) 185, 662
BssMI GATC 5 cut(s) 7, 85, 313, 463, 645
BssNI GRCGYC 2 cut(s) 343, 433
Bst2UI CCWGG 2 cut(s) 186, 664
Bst4CI ACNGT 2 cut(s) 241, 268
BstACI GRCGYC 2 cut(s) 343, 433
BstC8I GCNNGC 5 cut(s) 61, 306, 347, 412, 573
BstDEI CTNAG 4 cut(s) 50, 467, 639, 699
BstEII GGTNACC 1 cut(s) 666
BstF5I GGATG 3 cut(s) 10, 456, 499
BstH2I RGCGCY 1 cut(s) 224
BstHHI GCGC 1 cut(s) 223
BstKTI GATC 5 cut(s) 10, 88, 316, 466, 648
BstMAI GTCTC 1 cut(s) 506
BstMBI GATC 5 cut(s) 7, 85, 313, 463, 645
BstMWI GCNNNNNNNGC 3 cut(s) 60, 305, 351
BstNI CCWGG 2 cut(s) 186, 664
BstNSI RCATGY 1 cut(s) 250
BstPI GGTNACC 1 cut(s) 666
BstSCI CCNGG 2 cut(s) 184, 662
BstSFI CTRYAG 2 cut(s) 362, 678
BstV1I GCAGC 5 cut(s) 210, 348, 376, 412, 604
BstX2I RGATCY 2 cut(s) 313, 463
BstYI RGATCY 2 cut(s) 313, 463
BsuRI GGCC 4 cut(s) 150, 329, 410, 605
BtsCI GGATG 3 cut(s) 10, 456, 499
BtsI GCAGTG 1 cut(s) 299
BtsIMutI CAGTG 4 cut(s) 96, 237, 299, 696
BveI ACCTGC 1 cut(s) 685
Cac8I GCNNGC 5 cut(s) 61, 306, 347, 412, 573
CaiI CAGNNNCTG 1 cut(s) 686
CfoI GCGC 1 cut(s) 223
CseI GACGC 2 cut(s) 351, 422
Csp6I GTAC 1 cut(s) 120
CviAII CATG 3 cut(s) 216, 247, 402
CviQI GTAC 1 cut(s) 120
DdeI CTNAG 4 cut(s) 50, 467, 639, 699
DpnI GATC 5 cut(s) 9, 87, 315, 465, 647
DpnII GATC 5 cut(s) 7, 85, 313, 463, 645
EaeI YGGCCR 1 cut(s) 327
Eco57I CTGAAG 2 cut(s) 303, 330
Eco91I GGTNACC 1 cut(s) 666
EcoO65I GGTNACC 1 cut(s) 666
EcoRII CCWGG 2 cut(s) 184, 662
FaeI CATG 3 cut(s) 219, 250, 405
FaiI YATR 8 cut(s) 28, 69, 217, 248, 403, 419, 510, 659
FalI AAGNNNNNCTT 2 cut(s) 570, 602
FaqI GGGAC 1 cut(s) 203
FatI CATG 3 cut(s) 215, 246, 401
FbaI TGATCA 1 cut(s) 7
Fnu4HI GCNGC 6 cut(s) 224, 362, 365, 408, 426, 618
FokI GGATG 3 cut(s) 17, 463, 506
Fsp4HI GCNGC 6 cut(s) 224, 362, 365, 408, 426, 618
GlaI GCGC 1 cut(s) 222
GluI GCNGC 6 cut(s) 224, 362, 365, 408, 426, 618
HaeII RGCGCY 1 cut(s) 224
HaeIII GGCC 4 cut(s) 150, 329, 410, 605
HapII CCGG 1 cut(s) 151
HgaI GACGC 2 cut(s) 351, 422
HhaI GCGC 1 cut(s) 223
Hin1I GRCGYC 2 cut(s) 343, 433
Hin1II CATG 3 cut(s) 219, 250, 405
Hin6I GCGC 1 cut(s) 221
HinP1I GCGC 1 cut(s) 221
HincII GTYRAC 1 cut(s) 244
HindII GTYRAC 1 cut(s) 244
HindIII AAGCTT 1 cut(s) 291
HinfI GANTC 1 cut(s) 479
HpaII CCGG 1 cut(s) 151
HphI GGTGA 3 cut(s) 95, 212, 380
Hpy166II GTNNAC 2 cut(s) 32, 244
Hpy188I TCNGA 4 cut(s) 77, 283, 318, 642
Hpy188III TCNNGA 2 cut(s) 17, 125
Hpy8I GTNNAC 2 cut(s) 32, 244
Hpy99I CGWCG 1 cut(s) 438
HpyAV CCTTC 2 cut(s) 261, 441
HpyCH4III ACNGT 2 cut(s) 241, 268
HpyCH4IV ACGT 1 cut(s) 34
HpyCH4V TGCA 7 cut(s) 23, 215, 299, 349, 364, 620, 680
HpyF10VI GCNNNNNNNGC 3 cut(s) 60, 305, 351
HpyF3I CTNAG 4 cut(s) 50, 467, 639, 699
HpySE526I ACGT 1 cut(s) 34
Hsp92I GRCGYC 2 cut(s) 343, 433
Hsp92II CATG 3 cut(s) 219, 250, 405
HspAI GCGC 1 cut(s) 221
Ksp22I TGATCA 1 cut(s) 7
Kzo9I GATC 5 cut(s) 7, 85, 313, 463, 645
LmnI GCTCC 2 cut(s) 205, 231
Lsp1109I GCAGC 5 cut(s) 210, 348, 376, 412, 604
LweI GCATC 1 cut(s) 10
MaeII ACGT 1 cut(s) 34
MaeIII GTNAC 3 cut(s) 91, 592, 666
MalI GATC 5 cut(s) 9, 87, 315, 465, 647
MboI GATC 5 cut(s) 7, 85, 313, 463, 645
MboII GAAGA 9 cut(s) 154, 246, 323, 542, 545, 548, 551, 558, 655
MflI RGATCY 2 cut(s) 313, 463
MhlI GDGCHC 1 cut(s) 640
MlsI TGGCCA 1 cut(s) 329
MluCI AATT 4 cut(s) 166, 320, 335, 562
MluNI TGGCCA 1 cut(s) 329
MlyI GAGTC 1 cut(s) 488
Mox20I TGGCCA 1 cut(s) 329
MscI TGGCCA 1 cut(s) 329
MseI TTAA 1 cut(s) 179
Msp20I TGGCCA 1 cut(s) 329
MspI CCGG 1 cut(s) 151
MspR9I CCNGG 2 cut(s) 186, 664
MvaI CCWGG 2 cut(s) 186, 664
MwoI GCNNNNNNNGC 3 cut(s) 60, 305, 351
NdeII GATC 5 cut(s) 7, 85, 313, 463, 645
NlaIII CATG 3 cut(s) 219, 250, 405
NmuCI GTSAC 2 cut(s) 91, 592
NspI RCATGY 1 cut(s) 250
PkrI GCNGC 6 cut(s) 225, 363, 366, 409, 427, 619
PleI GAGTC 1 cut(s) 487
PpsI GAGTC 1 cut(s) 487
Psp6I CCWGG 2 cut(s) 184, 662
PspEI GGTNACC 1 cut(s) 666
PspGI CCWGG 2 cut(s) 184, 662
PstI CTGCAG 2 cut(s) 366, 682
PstNI CAGNNNCTG 1 cut(s) 686
PsuI RGATCY 2 cut(s) 313, 463
RsaI GTAC 1 cut(s) 121
RsaNI GTAC 1 cut(s) 120
SaqAI TTAA 1 cut(s) 179
SatI GCNGC 6 cut(s) 224, 362, 365, 408, 426, 618
Sau3AI GATC 5 cut(s) 7, 85, 313, 463, 645
SchI GAGTC 1 cut(s) 488
ScrFI CCNGG 2 cut(s) 186, 664
SduI GDGCHC 1 cut(s) 640
SfaNI GCATC 1 cut(s) 10
SfcI CTRYAG 2 cut(s) 362, 678
SmlI CTYRAG 2 cut(s) 472, 607
SmoI CTYRAG 2 cut(s) 472, 607
Sse9I AATT 4 cut(s) 166, 320, 335, 562
SsiI CCGC 2 cut(s) 198, 407
SspI AATATT 1 cut(s) 710
StyD4I CCNGG 2 cut(s) 184, 662
TaaI ACNGT 2 cut(s) 241, 268
TaiI ACGT 1 cut(s) 37
TaqI TCGA 1 cut(s) 126
TasI AATT 4 cut(s) 166, 320, 335, 562
TauI GCSGC 1 cut(s) 410
Tru1I TTAA 1 cut(s) 179
Tru9I TTAA 1 cut(s) 179
TscAI CASTG 4 cut(s) 96, 244, 306, 703
TseFI GTSAC 2 cut(s) 91, 592
TseI GCWGC 5 cut(s) 223, 361, 364, 425, 617
Tsp45I GTSAC 2 cut(s) 91, 592
TspDTI ATGAA 2 cut(s) 97, 406
TspRI CASTG 4 cut(s) 96, 244, 306, 703
XapI RAATTY 2 cut(s) 320, 562
XceI RCATGY 1 cut(s) 250
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.