Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr16

Orthogroup ID

OG0008944

Physical Location & Seq

Genomic Coordinates Forward (+)

33075740 .. 33082461

Gene Structure 6722 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD16G1264100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2169 bp

ATGGAATTGAGGAAGGAAAGGCTTGTCAGGTTTTATTCTGATGAAAAACGGCATAAAGAATCTTTATGGCGAAGAGCTGAGCCATCGCAGCTTGAAAAGTCATCATCTGCATATAAGTCATCTTCATTGCTTAAATCTGACACTAGACTAGTGGGGGGAAGAAATAGATTTGTTGAAACTTCCAGCATTGGGCATTCAAAGGTGTTTCCAGAGGACTATGAACCTTGGCAAAAGAGAATACTTGACCCGGGTAGTGAGATCGTGTTGCAATGGAATCGGGTTTTCATTGTCTCCTGCTTGGTGGCACTTTTTGTTGACCCATTGTATTTTTATCTGCCCATGCTGGGAGGGGATGCAAACACGTATTGTGTGAAGACGGACTTGAGGTTGCGAATTGTTGTGACTTGTTTCCGAACTATTGCGGATATCTTTTATATGTTGCATGTGATGATAAAGTTTAGGACAGCCTATGTTGCGCCAAGTTCTCGTGTTTTTGGGAGGGGTGAACTTGTCATGGACCCAAAGAAGATTGCGCAGAGATATATTAGATCTGACTTCTTCATTGATCTCATAGCCACATTGCCCCTCCCTCAGATAGTTATCTGGTTTGTTATCCCCGCAACAAGAAGCGCTCAAACGGATCATAACAACAATGCGCTTGCACTAATTGTTCTGATCCAATATGTCCCCAGATTATATCTTATTTTTCCGTTGAGTTTGCATATAATCAAAGCAACTGGAGTGGTGACAAAGACTGCATGGGCAGGGGCTGCATATAATCTTCTATTGTACATGCTGGCCAGCCATGTGTTAGGGGCAGCATGGTATTTGCTATCAATTGACCGATATACCTCATGCTGGAAGTCATTTTGTCAAAAGGAACTCAACACTACAAAATGCTCGCTTTCTTATCTAGATTGTGACAATCTTAACAGAAAAGGTTGGGTGAGTAGTACAAATGTGTTTGAGAATTGTAATCCTGCAAGCAAAAATTTCAAATATGGCATATTTGAAAGCGCAGTGACAAAAAATGTTGTCTCCTCAAACTTCATCACAAAATACTTCTATTGTTTATGGTGGGGCTTACAACAACTAAGTTCGTATGGGCAAAACTTGTCGACAAGCACTTTTATTGGGGAGACGTCGTTTGCCATTCTCATTGCCATTGTGGGTCTTGTATTATTTGCCCACTTAATTGGGAACATGCAGACTTATCTCCAATCAATCACTGTGAGGCTTGAGGAGTGGAGGCTTAAGCGAAGAGACACTGAGGAATGGATGAAGCATCGTCAACTCCCTCAAGATTTGAGACGACGTGTTCGTCGCTTTGTTCAATACAAATGGCTGGCAACTCGAGGAGTTGATGAAGAAGCCATCCTTCATGGCTTACCTGCTGATCTTCGTTGTGATATTCAACGCCACCTATGCTTAGATCTTGTTCGAAGAGTCCCGTTCTTCTCACAGATGGATGATCAACTTCTCGATGCAATATGTGAGCGTTTGGTGTCCTCCCTGAGCACGGGGGGTACCTACATAGTTCGAGAGGGTGACCCGGTGACAGAAATGCTTTTCATTATAAGAGGGGCTCTGGATAGTTCTACCACCAATGGAGGGCGGAGTGGATTCTTCAACTCGATCACATTAAAACCTGGAGACTTTTGTGGTGAGGAGCTGCTTGCATGGGCTTTGCTCCCAAAATCCACTGTTAATTTGCCATCTTCAACACGGACAGTTAAAGCACTTAGTGAAGTTGAAGCTTTTGCATTGCGAGCTGAAGATCTCAAATTTGTTGCTAACCAATTCAGACGCCTGCACAGCAAGAAGTTGCAGCACACATTTCGGTTTTACTCTCACCATTGGAGAACTTGGGCGGCCTGTTTCATACAGGCTGCTTGGCGCCGCCACAAGAGAAGAATGATGGTAAAAGATCTTTGTATGAGCGAATCATTTGCTTTGGATGAGAAAGTGGCTTATGAGACTGGGCAAGAGGAAGAACATCTTTCTGTTGTTTCAAATCCCGCTTCCCAGGTAAAAATGAATCTGGGGGTCACATTACTGGCTTCAAGATTTGCTGCAAACACAAGAAGGGGAGCTCAGAAGCTCAAGGACGAGATGCCAAAATTACGAAAACCTGAAGAACCGGACTTTTCCACTGAGCCTGAAGATTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005216 GO:0005244 GO:0005249 GO:0005261 GO:0005267 GO:0005575 GO:0005623 GO:0005886 GO:0005887 GO:0006810 GO:0006811 GO:0006812 GO:0006813 GO:0008150 GO:0008324 GO:0009987 GO:0015075 GO:0015077 GO:0015079 GO:0015267 GO:0015318 GO:0015672 GO:0016020 GO:0016021 GO:0022803 GO:0022832 GO:0022836 GO:0022838 GO:0022839 GO:0022843 GO:0022857 GO:0022890 GO:0030001 GO:0031224 GO:0031226 GO:0034220 GO:0042391 GO:0044425 GO:0044459 GO:0044464 GO:0046873 GO:0051179 GO:0051234 GO:0055085 GO:0065007 GO:0065008 GO:0071804 GO:0071805 GO:0071944 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

ko04626 map04626

Pfam Domains

Protein Families

Ion_trans cNMP_binding

Protein Analysis

723

Amino Acids

83.14

Weight (kDa)

9.31

Isoelectric Point (pI)

48.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Ion_trans	PF00520	91 - 412	2.8e-18	Ion transport protein
cNMP_binding	PF00027	509 - 597	2.9e-07	Cyclic nucleotide-binding domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008944)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G30360
fragaria_vesca	FvH4_4g09800
malus_domestica	MD13G1261400.v1.1 MD16G1264100.v1.1
prunus_persica	Prupe.1G095400_v2.0.a1 Prupe.1G095400_v2.0.a1 Prupe.1G095400_v2.0.a1 Prupe.1G095400_v2.0.a1 Prupe.1G095400_v2.0.a1
pyrus_communis	pycom13g24930 pycom16g23260
rosa_chinensis	RchiOBHm_Chr4g0404621
rosa_laevigata	RLG00000008918
rosa_multiflora	Rmu_sc0002699.1_g000026
rosa_roxburghii	Rroxscaffold_5G00348770
rosa_rugosa	Rorug04G0048000
rosa_samantha	Rh4AG124000 Rh4BG117700 Rh4BG117800 Rh4CG132100 Rh4DG116900
rosa_wichuraiana	Rw4G010050

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1577
AasI	GACNNNNNNGTC	1 cut(s)	1320
AatII	GACGTC	1 cut(s)	1145
Acc16I	TGCGCA	1 cut(s)	534
Acc36I	ACCTGC	1 cut(s)	1399
Acc65I	GGTACC	1 cut(s)	1526
AccB1I	GGYRCC	2 cut(s)	1526, 1896
AccI	GTMKAC	1 cut(s)	1118
AciI	CCGC	6 cut(s)	422, 618, 1615, 1871, 1900, 2019
AclWI	GGATC	2 cut(s)	648, 670
AcoI	YGGCCR	1 cut(s)	798
AcsI	RAATTY	2 cut(s)	991, 1784
AcuI	CTGAAG	2 cut(s)	1794, 2154
AcyI	GRCGYC	3 cut(s)	1142, 1807, 1897
AdeI	CACNNNGTG	1 cut(s)	1745
AfaI	GTAC	3 cut(s)	791, 955, 1528
AfeI	AGCGCT	1 cut(s)	631
AfiI	CCNNNNNNNGG	5 cut(s)	189, 344, 858, 1519, 1611
AflII	CTTAAG	1 cut(s)	1253
AflIII	ACRYGT	2 cut(s)	360, 1315
AhlI	ACTAGT	1 cut(s)	148
AjiI	CACGTC	1 cut(s)	1316
AjnI	CCWGG	2 cut(s)	1648, 2025
AluBI	AGCT	7 cut(s)	77, 91, 1672, 1757, 1772, 2093, 2101
AluI	AGCT	7 cut(s)	77, 91, 1672, 1757, 1772, 2093, 2101
Alw21I	GWGCWC	2 cut(s)	1520, 2095
Alw26I	GTCTC	7 cut(s)	295, 1042, 1133, 1257, 1303, 1647, 1970
AlwI	GGATC	2 cut(s)	648, 670
AlwNI	CAGNNNCTG	1 cut(s)	770
Ama87I	CYCGRG	2 cut(s)	247, 1353
Aor51HI	AGCGCT	1 cut(s)	631
AoxI	GGCC	2 cut(s)	798, 1872
ApeKI	GCWGC	7 cut(s)	88, 770, 818, 1672, 1828, 1889, 2072
ApoI	RAATTY	2 cut(s)	991, 1784
ArsI	GACNNNNNNTTYG	2 cut(s)	1302, 1334
Asp718I	GGTACC	1 cut(s)	1526
AspLEI	GCGC	6 cut(s)	478, 535, 632, 658, 1019, 1899
AspS9I	GGNCC	1 cut(s)	517
AsuC2I	CCSGG	3 cut(s)	248, 249, 1553
AsuHPI	GGTGA	7 cut(s)	515, 757, 958, 1559, 1567, 1676, 1844
AsuII	TTCGAA	1 cut(s)	1441
AvaI	CYCGRG	2 cut(s)	247, 1353
AvaII	GGWCC	1 cut(s)	517
BalI	TGGCCA	1 cut(s)	800
BanI	GGYRCC	2 cut(s)	1526, 1896
BanII	GRGCYC	2 cut(s)	1588, 2095
BauI	CACGAG	1 cut(s)	486
BbsI	GAAGAC	1 cut(s)	380
Bbv12I	GWGCWC	2 cut(s)	1520, 2095
BbvI	GCAGC	7 cut(s)	100, 757, 830, 1659, 1840, 1876, 2059
BccI	CCATC	5 cut(s)	91, 1382, 1459, 1723, 1912
BceAI	ACGGC	1 cut(s)	65
BcgI	CGANNNNNNTGC	6 cut(s)	257, 291, 1820, 1854, 1931, 1965
BciT130I	CCWGG	2 cut(s)	1650, 2027
BclI	TGATCA	1 cut(s)	1471
BcnI	CCSGG	3 cut(s)	248, 249, 1553
BcoDI	GTCTC	7 cut(s)	295, 1042, 1133, 1257, 1303, 1647, 1970
BcuI	ACTAGT	1 cut(s)	148
BfaI	CTAG	3 cut(s)	144, 149, 914
BfoI	RGCGCY	2 cut(s)	633, 1900
BfrI	CTTAAG	1 cut(s)	1253
BfuAI	ACCTGC	1 cut(s)	1399
BglII	AGATCT	4 cut(s)	548, 1432, 1777, 1927
BisI	GCNGC	9 cut(s)	89, 771, 819, 1673, 1829, 1872, 1890, 1900, 2073
BlpI	GCTNAGC	1 cut(s)	78
BlsI	GCNGC	9 cut(s)	90, 772, 820, 1674, 1830, 1873, 1891, 1901, 2074
Bme1390I	CCNGG	5 cut(s)	248, 249, 1553, 1650, 2027
Bme18I	GGWCC	1 cut(s)	517
BmeT110I	CYCGRG	2 cut(s)	247, 1353
BmgBI	CACGTC	1 cut(s)	1316
BmgT120I	GGNCC	1 cut(s)	517
BmiI	GGNNCC	3 cut(s)	519, 1528, 1898
BmrFI	CCNGG	5 cut(s)	248, 249, 1553, 1650, 2027
BmrI	ACTGGG	1 cut(s)	1989
BmsI	GCATC	4 cut(s)	343, 1294, 1474, 2103
BmuI	ACTGGG	1 cut(s)	1989
BpiI	GAAGAC	1 cut(s)	380
BpmI	CTGGAG	2 cut(s)	759, 1671
Bpu10I	CCTNAGC	1 cut(s)	1514
Bpu1102I	GCTNAGC	1 cut(s)	78
Bpu14I	TTCGAA	1 cut(s)	1441
BpuEI	CTTGAG	4 cut(s)	403, 1259, 1284, 2087
BpuMI	CCSGG	3 cut(s)	248, 249, 1553
BsaAI	YACGTR	1 cut(s)	363
BsaBI	GATNNNNATC	1 cut(s)	599
BsaHI	GRCGYC	3 cut(s)	1142, 1807, 1897
BsaJI	CCNNGG	3 cut(s)	224, 247, 2025
BsaWI	WCCGGW	1 cut(s)	2140
BsaXI	ACNNNNNCTCC	2 cut(s)	1130, 1160
Bsc4I	CCNNNNNNNGG	5 cut(s)	189, 344, 858, 1519, 1611
Bse1I	ACTGG	3 cut(s)	742, 1984, 2061
Bse3DI	GCAATG	5 cut(s)	125, 275, 578, 1158, 1763
Bse8I	GATNNNNATC	1 cut(s)	599
BseBI	CCWGG	2 cut(s)	1650, 2027
BseDI	CCNNGG	3 cut(s)	224, 247, 2025
BseGI	GGATG	5 cut(s)	358, 1284, 1374, 1474, 1963
BseJI	GATNNNNATC	1 cut(s)	599
BseLI	CCNNNNNNNGG	5 cut(s)	189, 344, 858, 1519, 1611
BseMI	GCAATG	5 cut(s)	125, 275, 578, 1158, 1763
BseMII	CTCAG	6 cut(s)	69, 605, 1260, 1505, 2108, 2145
BseNI	ACTGG	3 cut(s)	742, 1984, 2061
BseRI	GAGGAG	4 cut(s)	1030, 1256, 1371, 1682
BseXI	GCAGC	7 cut(s)	100, 757, 830, 1659, 1840, 1876, 2059
BseYI	CCCAGC	1 cut(s)	343
BsgI	GTGCAG	1 cut(s)	1796
BshFI	GGCC	2 cut(s)	800, 1874
BshNI	GGYRCC	2 cut(s)	1526, 1896
BsiHKAI	GWGCWC	2 cut(s)	1520, 2095
BsiHKCI	CYCGRG	2 cut(s)	247, 1353
BsiSI	CCGG	3 cut(s)	248, 1553, 2141
BslFI	GGGAC	2 cut(s)	671, 1433
BslI	CCNNNNNNNGG	5 cut(s)	189, 344, 858, 1519, 1611
BsmAI	GTCTC	7 cut(s)	295, 1042, 1133, 1257, 1303, 1647, 1970
BsmBI	CGTCTC	2 cut(s)	1133, 1303
BsmFI	GGGAC	2 cut(s)	671, 1433
BsmI	GAATGC	1 cut(s)	193
BsnI	GGCC	2 cut(s)	800, 1874
BsoBI	CYCGRG	2 cut(s)	247, 1353
Bsp119I	TTCGAA	1 cut(s)	1441
Bsp1286I	GDGCHC	3 cut(s)	1520, 1588, 2095
Bsp1407I	TGTACA	1 cut(s)	789
Bsp1720I	GCTNAGC	1 cut(s)	78
BspACI	CCGC	6 cut(s)	422, 618, 1615, 1871, 1900, 2019
BspANI	GGCC	2 cut(s)	800, 1874
BspCNI	CTCAG	6 cut(s)	70, 604, 1261, 1506, 2107, 2146
BspLI	GGNNCC	3 cut(s)	519, 1528, 1898
BspMI	ACCTGC	1 cut(s)	1399
BspPI	GGATC	2 cut(s)	648, 670
BspQI	GCTCTTC	1 cut(s)	67
BspT104I	TTCGAA	1 cut(s)	1441
BspT107I	GGYRCC	2 cut(s)	1526, 1896
BspTI	CTTAAG	1 cut(s)	1253
BsrDI	GCAATG	5 cut(s)	125, 275, 578, 1158, 1763
BsrGI	TGTACA	1 cut(s)	789
BsrI	ACTGG	3 cut(s)	742, 1984, 2061
BssECI	CCNNGG	3 cut(s)	224, 247, 2025
BssNI	GRCGYC	3 cut(s)	1142, 1807, 1897
BssSI	CACGAG	1 cut(s)	486
BssT1I	CCWWGG	1 cut(s)	224
Bst2BI	CACGAG	1 cut(s)	486
Bst2UI	CCWGG	2 cut(s)	1650, 2027
Bst4CI	ACNGT	3 cut(s)	1231, 1705, 1732
Bst6I	CTCTTC	3 cut(s)	67, 1255, 1438
BstACI	GRCGYC	3 cut(s)	1142, 1807, 1897
BstAFI	CTTAAG	1 cut(s)	1253
BstAPI	GCANNNNNTGC	1 cut(s)	770
BstAUI	TGTACA	1 cut(s)	789
BstBAI	YACGTR	1 cut(s)	363
BstBI	TTCGAA	1 cut(s)	1441
BstC8I	GCNNGC	9 cut(s)	660, 798, 802, 902, 985, 1347, 1677, 1770, 1811
BstDEI	CTNAG	9 cut(s)	78, 591, 1094, 1269, 1429, 1514, 1742, 2094, 2154
BstEII	GGTNACC	1 cut(s)	1547
BstF5I	GGATG	5 cut(s)	358, 1284, 1374, 1474, 1963
BstH2I	RGCGCY	2 cut(s)	633, 1900
BstHHI	GCGC	6 cut(s)	478, 535, 632, 658, 1019, 1899
BstMAI	GTCTC	7 cut(s)	295, 1042, 1133, 1257, 1303, 1647, 1970
BstMWI	GCNNNNNNNGC	6 cut(s)	88, 473, 770, 1425, 1769, 1815
BstNI	CCWGG	2 cut(s)	1650, 2027
BstNSI	RCATGY	3 cut(s)	446, 796, 1207
BstPI	GGTNACC	1 cut(s)	1547
BstSCI	CCNGG	5 cut(s)	246, 247, 1551, 1648, 2025
BstV1I	GCAGC	7 cut(s)	100, 757, 830, 1659, 1840, 1876, 2059
BstV2I	GAAGAC	1 cut(s)	380
BstX2I	RGATCY	4 cut(s)	548, 1432, 1777, 1927
BstXI	CCANNNNNNTGG	1 cut(s)	1196
BstYI	RGATCY	4 cut(s)	548, 1432, 1777, 1927
BsuRI	GGCC	2 cut(s)	800, 1874
BtgZI	GCGATG	1 cut(s)	69
BtrI	CACGTC	1 cut(s)	1316
BtsCI	GGATG	5 cut(s)	358, 1284, 1374, 1474, 1963
BtsI	GCAGTG	1 cut(s)	1026
BtsIMutI	CAGTG	5 cut(s)	1026, 1227, 1266, 1701, 2151
BveI	ACCTGC	1 cut(s)	1399
Cac8I	GCNNGC	9 cut(s)	660, 798, 802, 902, 985, 1347, 1677, 1770, 1811
CaiI	CAGNNNCTG	1 cut(s)	770
CfoI	GCGC	6 cut(s)	478, 535, 632, 658, 1019, 1899
Cfr13I	GGNCC	1 cut(s)	517
Cfr9I	CCCGGG	1 cut(s)	247
CseI	GACGC	1 cut(s)	1815
Csp6I	GTAC	3 cut(s)	790, 954, 1527
CspCI	CAANNNNNGTGG	2 cut(s)	723, 758
CviQI	GTAC	3 cut(s)	790, 954, 1527
DdeI	CTNAG	9 cut(s)	78, 591, 1094, 1269, 1429, 1514, 1742, 2094, 2154
DinI	GGCGCC	1 cut(s)	1898
DraIII	CACNNNGTG	1 cut(s)	1745
DrdI	GACNNNNNNGTC	1 cut(s)	1320
DseDI	GACNNNNNNGTC	1 cut(s)	1320
EaeI	YGGCCR	1 cut(s)	798
Eam1104I	CTCTTC	3 cut(s)	67, 1255, 1438
EarI	CTCTTC	3 cut(s)	67, 1255, 1438
EciI	GGCGGA	1 cut(s)	1630
Ecl136II	GAGCTC	1 cut(s)	2093
Eco130I	CCWWGG	1 cut(s)	224
Eco24I	GRGCYC	2 cut(s)	1588, 2095
Eco32I	GATATC	1 cut(s)	427
Eco47I	GGWCC	1 cut(s)	517
Eco47III	AGCGCT	1 cut(s)	631
Eco53kI	GAGCTC	1 cut(s)	2093
Eco57I	CTGAAG	2 cut(s)	1794, 2154
Eco88I	CYCGRG	2 cut(s)	247, 1353
Eco91I	GGTNACC	1 cut(s)	1547
EcoICRI	GAGCTC	1 cut(s)	2093
EcoO65I	GGTNACC	1 cut(s)	1547
EcoRII	CCWGG	2 cut(s)	1648, 2025
EcoRV	GATATC	1 cut(s)	427
EcoT14I	CCWWGG	1 cut(s)	224
EcoT38I	GRGCYC	2 cut(s)	1588, 2095
EgeI	GGCGCC	1 cut(s)	1898
EheI	GGCGCC	1 cut(s)	1898
ErhI	CCWWGG	1 cut(s)	224
Esp3I	CGTCTC	2 cut(s)	1133, 1303
FalI	AAGNNNNNCTT	6 cut(s)	365, 397, 1362, 1394, 1983, 2015
FaqI	GGGAC	2 cut(s)	671, 1433
FauI	CCCGC	2 cut(s)	625, 2026
FbaI	TGATCA	1 cut(s)	1471
FblI	GTMKAC	1 cut(s)	1118
Fnu4HI	GCNGC	9 cut(s)	89, 771, 819, 1673, 1829, 1872, 1890, 1900, 2073
FokI	GGATG	5 cut(s)	365, 1291, 1361, 1481, 1970
FriOI	GRGCYC	2 cut(s)	1588, 2095
Fsp4HI	GCNGC	9 cut(s)	89, 771, 819, 1673, 1829, 1872, 1890, 1900, 2073
FspBI	CTAG	3 cut(s)	144, 149, 914
FspI	TGCGCA	1 cut(s)	534
GlaI	GCGC	6 cut(s)	477, 534, 631, 657, 1018, 1898
GluI	GCNGC	9 cut(s)	89, 771, 819, 1673, 1829, 1872, 1890, 1900, 2073
GsaI	CCCAGC	1 cut(s)	347
GsuI	CTGGAG	2 cut(s)	759, 1671
HaeII	RGCGCY	2 cut(s)	633, 1900
HaeIII	GGCC	2 cut(s)	800, 1874
HapII	CCGG	3 cut(s)	248, 1553, 2141
HgaI	GACGC	1 cut(s)	1815
HhaI	GCGC	6 cut(s)	478, 535, 632, 658, 1019, 1899
Hin1I	GRCGYC	3 cut(s)	1142, 1807, 1897
Hin6I	GCGC	6 cut(s)	476, 533, 630, 656, 1017, 1897
HinP1I	GCGC	6 cut(s)	476, 533, 630, 656, 1017, 1897
HincII	GTYRAC	3 cut(s)	316, 1119, 1292
HindII	GTYRAC	3 cut(s)	316, 1119, 1292
HindIII	AAGCTT	1 cut(s)	1755
HinfI	GANTC	6 cut(s)	59, 274, 1446, 1623, 1943, 2038
HpaII	CCGG	3 cut(s)	248, 1553, 2141
HphI	GGTGA	7 cut(s)	515, 757, 958, 1559, 1567, 1676, 1844
Hpy166II	GTNNAC	4 cut(s)	316, 506, 1119, 1292
Hpy188I	TCNGA	8 cut(s)	40, 139, 413, 553, 594, 675, 1805, 2097
Hpy188III	TCNNGA	7 cut(s)	209, 914, 1301, 1481, 1541, 1589, 2064
Hpy8I	GTNNAC	4 cut(s)	316, 506, 1119, 1292
Hpy99I	CGWCG	3 cut(s)	1147, 1317, 1326
HpyAV	CCTTC	3 cut(s)	7, 1388, 2079
HpyCH4III	ACNGT	3 cut(s)	1231, 1705, 1732
HpyCH4IV	ACGT	3 cut(s)	362, 1142, 1315
HpyF10VI	GCNNNNNNNGC	6 cut(s)	88, 473, 770, 1425, 1769, 1815
HpyF3I	CTNAG	9 cut(s)	78, 591, 1094, 1269, 1429, 1514, 1742, 2094, 2154
HpySE526I	ACGT	3 cut(s)	362, 1142, 1315
Hsp92I	GRCGYC	3 cut(s)	1142, 1807, 1897
HspAI	GCGC	6 cut(s)	476, 533, 630, 656, 1017, 1897
KasI	GGCGCC	1 cut(s)	1896
KpnI	GGTACC	1 cut(s)	1530
Ksp22I	TGATCA	1 cut(s)	1471
LguI	GCTCTTC	1 cut(s)	67
LmnI	GCTCC	3 cut(s)	1669, 1695, 2090
Lsp1109I	GCAGC	7 cut(s)	100, 757, 830, 1659, 1840, 1876, 2059
LweI	GCATC	4 cut(s)	343, 1294, 1474, 2103
MaeI	CTAG	3 cut(s)	144, 149, 914
MaeII	ACGT	3 cut(s)	362, 1142, 1315
MaeIII	GTNAC	7 cut(s)	400, 745, 920, 1021, 1547, 1555, 2047
MfeI	CAATTG	1 cut(s)	837
MflI	RGATCY	4 cut(s)	548, 1432, 1777, 1927
MhlI	GDGCHC	3 cut(s)	1520, 1588, 2095
MlsI	TGGCCA	1 cut(s)	800
MluNI	TGGCCA	1 cut(s)	800
Mly113I	GGCGCC	1 cut(s)	1897
MlyI	GAGTC	1 cut(s)	1455
Mox20I	TGGCCA	1 cut(s)	800
MscI	TGGCCA	1 cut(s)	800
MseI	TTAA	7 cut(s)	132, 930, 1193, 1254, 1643, 1707, 1734
Msp20I	TGGCCA	1 cut(s)	800
MspCI	CTTAAG	1 cut(s)	1253
MspI	CCGG	3 cut(s)	248, 1553, 2141
MspR9I	CCNGG	5 cut(s)	248, 249, 1553, 1650, 2027
MunI	CAATTG	1 cut(s)	837
Mva1269I	GAATGC	1 cut(s)	193
MvaI	CCWGG	2 cut(s)	1650, 2027
MwoI	GCNNNNNNNGC	6 cut(s)	88, 473, 770, 1425, 1769, 1815
NarI	GGCGCC	1 cut(s)	1897
NciI	CCSGG	3 cut(s)	248, 249, 1553
NlaIV	GGNNCC	3 cut(s)	519, 1528, 1898
NmuCI	GTSAC	7 cut(s)	400, 745, 920, 1021, 1547, 1555, 2047
NsbI	TGCGCA	1 cut(s)	534
NspI	RCATGY	3 cut(s)	446, 796, 1207
NspV	TTCGAA	1 cut(s)	1441
PaeR7I	CTCGAG	1 cut(s)	1353
PciSI	GCTCTTC	1 cut(s)	67
PcsI	WCGNNNNNNNCGW	1 cut(s)	1318
PctI	GAATGC	1 cut(s)	193
PfeI	GAWTC	5 cut(s)	59, 274, 1623, 1943, 2038
PkrI	GCNGC	9 cut(s)	90, 772, 820, 1674, 1830, 1873, 1891, 1901, 2074
PleI	GAGTC	1 cut(s)	1454
PluTI	GGCGCC	1 cut(s)	1900
PpsI	GAGTC	1 cut(s)	1454
Ppu21I	YACGTR	1 cut(s)	363
PsiI	TTATAA	1 cut(s)	1577
Psp124BI	GAGCTC	1 cut(s)	2095
Psp6I	CCWGG	2 cut(s)	1648, 2025
PspEI	GGTNACC	1 cut(s)	1547
PspFI	CCCAGC	1 cut(s)	343
PspGI	CCWGG	2 cut(s)	1648, 2025
PspN4I	GGNNCC	3 cut(s)	519, 1528, 1898
PspPI	GGNCC	1 cut(s)	517
PspXI	VCTCGAGB	1 cut(s)	1353
PstNI	CAGNNNCTG	1 cut(s)	770
PsuI	RGATCY	4 cut(s)	548, 1432, 1777, 1927
RsaI	GTAC	3 cut(s)	791, 955, 1528
RsaNI	GTAC	3 cut(s)	790, 954, 1527
SacI	GAGCTC	1 cut(s)	2095
SalI	GTCGAC	1 cut(s)	1117
SapI	GCTCTTC	1 cut(s)	67
SaqAI	TTAA	7 cut(s)	132, 930, 1193, 1254, 1643, 1707, 1734
SatI	GCNGC	9 cut(s)	89, 771, 819, 1673, 1829, 1872, 1890, 1900, 2073
Sau96I	GGNCC	1 cut(s)	517
SchI	GAGTC	1 cut(s)	1455
ScrFI	CCNGG	5 cut(s)	248, 249, 1553, 1650, 2027
SduI	GDGCHC	3 cut(s)	1520, 1588, 2095
SfaNI	GCATC	4 cut(s)	343, 1294, 1474, 2103
SfoI	GGCGCC	1 cut(s)	1898
Sfr274I	CTCGAG	1 cut(s)	1353
SfuI	TTCGAA	1 cut(s)	1441
SinI	GGWCC	1 cut(s)	517
SlaI	CTCGAG	1 cut(s)	1353
SmaI	CCCGGG	1 cut(s)	249
SmlI	CTYRAG	6 cut(s)	382, 1238, 1253, 1299, 1353, 2102
SmoI	CTYRAG	6 cut(s)	382, 1238, 1253, 1299, 1353, 2102
SpeI	ACTAGT	1 cut(s)	148
SsiI	CCGC	6 cut(s)	422, 618, 1615, 1871, 1900, 2019
SspDI	GGCGCC	1 cut(s)	1896
SspMI	CTAG	3 cut(s)	144, 149, 914
SstI	GAGCTC	1 cut(s)	2095
StyD4I	CCNGG	5 cut(s)	246, 247, 1551, 1648, 2025
StyI	CCWWGG	1 cut(s)	224
TaaI	ACNGT	3 cut(s)	1231, 1705, 1732
TaiI	ACGT	3 cut(s)	365, 1145, 1318
TaqI	TCGA	6 cut(s)	1118, 1354, 1441, 1482, 1540, 1634
TaqII	GACCGA	1 cut(s)	858
TatI	WGTACW	2 cut(s)	789, 953
TauI	GCSGC	2 cut(s)	1874, 1902
TfiI	GAWTC	5 cut(s)	59, 274, 1623, 1943, 2038
Tru1I	TTAA	7 cut(s)	132, 930, 1193, 1254, 1643, 1707, 1734
Tru9I	TTAA	7 cut(s)	132, 930, 1193, 1254, 1643, 1707, 1734
TscAI	CASTG	5 cut(s)	1026, 1234, 1273, 1708, 2158
TseFI	GTSAC	7 cut(s)	400, 745, 920, 1021, 1547, 1555, 2047
TseI	GCWGC	7 cut(s)	88, 770, 818, 1672, 1828, 1889, 2072
Tsp45I	GTSAC	7 cut(s)	400, 745, 920, 1021, 1547, 1555, 2047
TspGWI	ACGGA	4 cut(s)	392, 653, 699, 1741
TspMI	CCCGGG	1 cut(s)	247
TspRI	CASTG	5 cut(s)	1026, 1234, 1273, 1708, 2158
Vha464I	CTTAAG	1 cut(s)	1253
VpaK11BI	GGWCC	1 cut(s)	517
XapI	RAATTY	2 cut(s)	991, 1784
XbaI	TCTAGA	1 cut(s)	913
XceI	RCATGY	3 cut(s)	446, 796, 1207
XhoI	CTCGAG	1 cut(s)	1353
XmaI	CCCGGG	1 cut(s)	247
XmiI	GTMKAC	1 cut(s)	1118
XspI	CTAG	3 cut(s)	144, 149, 914
ZraI	GACGTC	1 cut(s)	1143

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.