Rh4DG116900

cyclic nucleotide-gated ion channel

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0008944
Physical Location & Seq
Reverse (-)
18544226 .. 18550226
6001 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG116900.1

Sequence Viewer

Length: 1575 bp
ATGGAATTGAAGAAGGAGAGGCTTGTCAGGTTTTATTCTGATAAAAAACACCATAGAGATTCTATATGGGACAGAACTGAGCCGTATCGTCTCGAAAAGTCGGCCTCTGCATATAGGGTTGAATCATCTTCGTTGCTCAAATCTGATAATGGACTAGTGGGGGGCACACGCAGATTTGCCGAAAATTTCAGAATCGGGCATTCAAAGGTGTTTCCAGAAGACCATGAGCCGTGGCGCAAGAGGATACTTGACCCAGGTAGTGAAATAGTGCTGCAATGGAATCGAGTTTTCATTGTCTCGTGCTTGGTTGCACTTTTTGTGGACCCATTGTACTTTTACTTGCCCATGGCTGAAGGGAGTGAAAACAGTTTTTGCATGAAGACAGACTTGAATTTGCGAATTATTGTGACCTTTTTCCGGACTATTGCGGATTTCTTCTATTTTTTGCACATGATTATAAAGTTTAGGACGGCTTATGTTGCACCAAGCTCCCGCGTTTTCGGGAGGGGTGAACTTGTCATGAACCCAAAGAAGATTGCACAGAGATACATTACATCCGACTTCTCCATTGATCTCATAGCCACGTTGCCACTTCCTCAGATAGTCATCTGGTTTATCATACCTCCCCAAAATGCTCAAACGGATAACACCAACAATGCACTAGCACTAATTGTTCTCCTCCAGTACATCCCCAGATTATATCTGATTTTCCCTTTAAGTTTGCAATTAATCAAAGCAACTGGAGTGGTGACAAAAACTGCTTGGGCAGGGGCTGCATATAATCTGCTATTATACATGCTGGCCAGCCATGTTTTAGGGGCAGCATGGTATTTGCTATCTATTGACCGGTATGCCTCATGCTGGAAAACATTTTGTAAAAAGGAACTCAGCCCCGTAAAATGCTTGCCTAACTATTTAGATTGTAATACTGTTGGCCAAGTTCCTCGCAAGACATGGGCAAATGGTACAAATGTTTTTCAAAATTGCAATCCTGAAGGTAACATATTCAAATATGGAATATTTGGAACTGCACTCACAAAACATGCTGTTTCGTCCAACTTCATCACAAAGTACTTCTATTGCTTATGGTGGGGTTTACAGCAACTAAGTTCGTATGGGCAAAATTTGACAACGAGCACTTTTATTGGAGAGACATCATTTGCCATTCTCATTGCGATTGTAGGTCTCGTCTTATTTGCCCACTTAATAGGGAATATGCAGACCTATCTCCAATCAATTACTGTGAGGCTTGAGGAGTGGAGGCTTAAGAGAAGAGACACTGAGGAATGGATGGGGCATCGTCAACTCCCAGAAGATTTGAGACAACGTGTTCGACGGTTTGACCAATACAAGTGGCTGGCCACTCGAGGAGTTGATGAAGAAGCCATCCTTCATGGCTTACCTGCTGATCTTCGCCATGATATTCAACGCCACCTATGCTTAGATCTTGTTCGACGAGTATGGTTCTCCTTAAGTATTTCTCTTTCATGGCTTACCAATATTCTTTGTCATATGCTATTATGGTCTAAGACTCTAAGAGTATTGCTTGAAGGTATAGTTGGGGCATACCGCTAA

Protein Analysis

524

Amino Acids

60.78

Weight (kDa)

9.41

Isoelectric Point (pI)

38.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ion_trans PF00520 93 - 416 6.8e-20 Ion transport protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 458
Acc36I ACCTGC 1 cut(s) 1411
AccII CGCG 1 cut(s) 495
AccIII TCCGGA 1 cut(s) 417
AciI CCGC 3 cut(s) 428, 493, 1570
AcoI YGGCCR 3 cut(s) 801, 934, 1359
AcsI RAATTY 3 cut(s) 184, 391, 1123
AcuI CTGAAG 2 cut(s) 372, 1014
AfaI GTAC 4 cut(s) 332, 686, 967, 1073
AfiI CCNNNNNNNGG 2 cut(s) 417, 861
AflII CTTAAG 2 cut(s) 1265, 1471
AflIII ACRYGT 1 cut(s) 1327
AgeI ACCGGT 1 cut(s) 846
AgsI TTSAA 8 cut(s) 10, 122, 204, 391, 980, 1009, 1427, 1550
AhlI ACTAGT 1 cut(s) 154
AjnI CCWGG 1 cut(s) 253
AjuI GAANNNNNNNTTGG 2 cut(s) 1542, 1574
AluBI AGCT 1 cut(s) 489
AluI AGCT 1 cut(s) 489
Alw21I GWGCWC 1 cut(s) 1139
Alw26I GTCTC 6 cut(s) 95, 301, 1145, 1190, 1269, 1315
AlwNI CAGNNNCTG 1 cut(s) 773
Ama87I CYCGRG 1 cut(s) 1365
Aor13HI TCCGGA 1 cut(s) 417
AoxI GGCC 4 cut(s) 102, 801, 934, 1359
ApeKI GCWGC 3 cut(s) 271, 773, 821
ApoI RAATTY 3 cut(s) 184, 391, 1123
ArsI GACNNNNNNTTYG 2 cut(s) 1314, 1346
AseI ATTAAT 1 cut(s) 728
AsiGI ACCGGT 1 cut(s) 846
AspLEI GCGC 1 cut(s) 237
AspS9I GGNCC 1 cut(s) 322
AsuHPI GGTGA 2 cut(s) 521, 760
AvaI CYCGRG 1 cut(s) 1365
AvaII GGWCC 1 cut(s) 322
BaeGI GKGCMC 1 cut(s) 167
BaeI ACNNNNGTAYC 2 cut(s) 957, 990
BalI TGGCCA 3 cut(s) 803, 936, 1361
BauI CACGAG 1 cut(s) 298
BbsI GAAGAC 2 cut(s) 225, 386
Bbv12I GWGCWC 1 cut(s) 1139
BbvI GCAGC 3 cut(s) 258, 760, 833
BccI CCATC 2 cut(s) 1285, 1394
BceAI ACGGC 3 cut(s) 67, 214, 486
BcgI CGANNNNNNTGC 2 cut(s) 263, 297
BciT130I CCWGG 1 cut(s) 255
BciVI GTATCC 1 cut(s) 237
BcoDI GTCTC 6 cut(s) 95, 301, 1145, 1190, 1269, 1315
BcuI ACTAGT 1 cut(s) 154
BfaI CTAG 2 cut(s) 155, 662
BfrI CTTAAG 2 cut(s) 1265, 1471
BfuAI ACCTGC 1 cut(s) 1411
BfuI GTATCC 1 cut(s) 237
BglII AGATCT 1 cut(s) 1444
BisI GCNGC 3 cut(s) 272, 774, 822
BlsI GCNGC 3 cut(s) 273, 775, 823
BmcAI AGTACT 1 cut(s) 1073
Bme1390I CCNGG 1 cut(s) 255
Bme18I GGWCC 1 cut(s) 322
BmeT110I CYCGRG 1 cut(s) 1365
BmgT120I GGNCC 1 cut(s) 322
BmiI GGNNCC 1 cut(s) 324
BmrFI CCNGG 1 cut(s) 255
BmsI GCATC 1 cut(s) 1306
BpiI GAAGAC 2 cut(s) 225, 386
BpmI CTGGAG 2 cut(s) 665, 762
BpuEI CTTGAG 1 cut(s) 1271
BsaBI GATNNNNATC 1 cut(s) 605
BsaI GGTCTC 1 cut(s) 1190
BsaJI CCNNGG 3 cut(s) 230, 253, 345
BsaWI WCCGGW 2 cut(s) 417, 846
Bsc4I CCNNNNNNNGG 2 cut(s) 417, 861
Bse118I RCCGGY 1 cut(s) 846
Bse1I ACTGG 2 cut(s) 682, 745
Bse3DI GCAATG 2 cut(s) 281, 1170
Bse8I GATNNNNATC 1 cut(s) 605
BseAI TCCGGA 1 cut(s) 417
BseBI CCWGG 1 cut(s) 255
BseDI CCNNGG 3 cut(s) 230, 253, 345
BseGI GGATG 4 cut(s) 554, 687, 1296, 1386
BseJI GATNNNNATC 1 cut(s) 605
BseLI CCNNNNNNNGG 2 cut(s) 417, 861
BseMI GCAATG 2 cut(s) 281, 1170
BseMII CTCAG 4 cut(s) 69, 611, 901, 1272
BseNI ACTGG 2 cut(s) 682, 745
BseRI GAGGAG 3 cut(s) 668, 1268, 1383
BseSI GKGCMC 1 cut(s) 167
BseXI GCAGC 3 cut(s) 258, 760, 833
BsgI GTGCAG 1 cut(s) 1014
Bsh1236I CGCG 1 cut(s) 495
BshFI GGCC 4 cut(s) 104, 803, 936, 1361
BshTI ACCGGT 1 cut(s) 846
BsiHKAI GWGCWC 1 cut(s) 1139
BsiHKCI CYCGRG 1 cut(s) 1365
BsiSI CCGG 2 cut(s) 418, 847
BslFI GGGAC 1 cut(s) 83
BslI CCNNNNNNNGG 2 cut(s) 417, 861
BsmAI GTCTC 6 cut(s) 95, 301, 1145, 1190, 1269, 1315
BsmBI CGTCTC 1 cut(s) 95
BsmFI GGGAC 1 cut(s) 83
BsmI GAATGC 1 cut(s) 199
BsnI GGCC 4 cut(s) 104, 803, 936, 1361
Bso31I GGTCTC 1 cut(s) 1190
BsoBI CYCGRG 1 cut(s) 1365
Bsp1286I GDGCHC 2 cut(s) 167, 1139
Bsp13I TCCGGA 1 cut(s) 417
Bsp143I GATC 3 cut(s) 571, 1408, 1444
Bsp19I CCATGG 1 cut(s) 345
BspACI CCGC 3 cut(s) 428, 493, 1570
BspANI GGCC 4 cut(s) 104, 803, 936, 1361
BspCNI CTCAG 4 cut(s) 70, 610, 900, 1273
BspEI TCCGGA 1 cut(s) 417
BspFNI CGCG 1 cut(s) 495
BspHI TCATGA 1 cut(s) 519
BspLI GGNNCC 1 cut(s) 324
BspMI ACCTGC 1 cut(s) 1411
BspTI CTTAAG 2 cut(s) 1265, 1471
BspTNI GGTCTC 1 cut(s) 1190
BsrDI GCAATG 2 cut(s) 281, 1170
BsrFI RCCGGY 1 cut(s) 846
BsrI ACTGG 2 cut(s) 682, 745
BssAI RCCGGY 1 cut(s) 846
BssECI CCNNGG 3 cut(s) 230, 253, 345
BssMI GATC 3 cut(s) 571, 1408, 1444
BssSI CACGAG 1 cut(s) 298
BssT1I CCWWGG 1 cut(s) 345
Bst2BI CACGAG 1 cut(s) 298
Bst2UI CCWGG 1 cut(s) 255
Bst4CI ACNGT 4 cut(s) 368, 931, 1243, 1338
Bst6I CTCTTC 1 cut(s) 1267
BstAFI CTTAAG 2 cut(s) 1265, 1471
BstAPI GCANNNNNTGC 1 cut(s) 773
BstC8I GCNNGC 4 cut(s) 801, 805, 905, 1359
BstDEI CTNAG 8 cut(s) 78, 597, 887, 1106, 1281, 1441, 1527, 1535
BstDSI CCRYGG 2 cut(s) 230, 345
BstF5I GGATG 4 cut(s) 554, 687, 1296, 1386
BstFNI CGCG 1 cut(s) 495
BstHHI GCGC 1 cut(s) 237
BstKTI GATC 3 cut(s) 574, 1411, 1447
BstMAI GTCTC 6 cut(s) 95, 301, 1145, 1190, 1269, 1315
BstMBI GATC 3 cut(s) 571, 1408, 1444
BstMWI GCNNNNNNNGC 3 cut(s) 479, 773, 1437
BstNI CCWGG 1 cut(s) 255
BstNSI RCATGY 2 cut(s) 799, 1046
BstSCI CCNGG 1 cut(s) 253
BstSLI GKGCMC 1 cut(s) 167
BstUI CGCG 1 cut(s) 495
BstV1I GCAGC 3 cut(s) 258, 760, 833
BstV2I GAAGAC 2 cut(s) 225, 386
BstX2I RGATCY 1 cut(s) 1444
BstYI RGATCY 1 cut(s) 1444
BsuI GTATCC 1 cut(s) 237
BsuRI GGCC 4 cut(s) 104, 803, 936, 1361
BtgI CCRYGG 2 cut(s) 230, 345
BtsCI GGATG 4 cut(s) 554, 687, 1296, 1386
BtsIMutI CAGTG 1 cut(s) 1278
BveI ACCTGC 1 cut(s) 1411
Cac8I GCNNGC 4 cut(s) 801, 805, 905, 1359
CaiI CAGNNNCTG 1 cut(s) 773
CciI TCATGA 1 cut(s) 519
CfoI GCGC 1 cut(s) 237
Cfr10I RCCGGY 1 cut(s) 846
Cfr13I GGNCC 1 cut(s) 322
Csp6I GTAC 4 cut(s) 331, 685, 966, 1072
CspAI ACCGGT 1 cut(s) 846
CspCI CAANNNNNGTGG 4 cut(s) 726, 761, 1334, 1369
CviQI GTAC 4 cut(s) 331, 685, 966, 1072
DdeI CTNAG 8 cut(s) 78, 597, 887, 1106, 1281, 1441, 1527, 1535
DpnI GATC 3 cut(s) 573, 1410, 1446
DpnII GATC 3 cut(s) 571, 1408, 1444
EaeI YGGCCR 3 cut(s) 801, 934, 1359
Eam1104I CTCTTC 1 cut(s) 1267
EarI CTCTTC 1 cut(s) 1267
Eco130I CCWWGG 1 cut(s) 345
Eco31I GGTCTC 1 cut(s) 1190
Eco47I GGWCC 1 cut(s) 322
Eco57I CTGAAG 2 cut(s) 372, 1014
Eco88I CYCGRG 1 cut(s) 1365
EcoRII CCWGG 1 cut(s) 253
EcoT14I CCWWGG 1 cut(s) 345
ErhI CCWWGG 1 cut(s) 345
Esp3I CGTCTC 1 cut(s) 95
FalI AAGNNNNNCTT 4 cut(s) 371, 403, 1374, 1406
FaqI GGGAC 1 cut(s) 83
FauI CCCGC 1 cut(s) 500
FauNDI CATATG 1 cut(s) 1512
Fnu4HI GCNGC 3 cut(s) 272, 774, 822
FokI GGATG 4 cut(s) 541, 674, 1303, 1373
Fsp4HI GCNGC 3 cut(s) 272, 774, 822
FspBI CTAG 2 cut(s) 155, 662
GlaI GCGC 1 cut(s) 236
GluI GCNGC 3 cut(s) 272, 774, 822
GsuI CTGGAG 2 cut(s) 665, 762
HaeIII GGCC 4 cut(s) 104, 803, 936, 1361
HapII CCGG 2 cut(s) 418, 847
HhaI GCGC 1 cut(s) 237
Hin6I GCGC 1 cut(s) 235
HinP1I GCGC 1 cut(s) 235
HincII GTYRAC 1 cut(s) 1304
HindII GTYRAC 1 cut(s) 1304
HinfI GANTC 5 cut(s) 59, 122, 192, 280, 1531
HpaII CCGG 2 cut(s) 418, 847
HphI GGTGA 2 cut(s) 521, 760
Hpy166II GTNNAC 4 cut(s) 322, 512, 1097, 1304
Hpy188I TCNGA 6 cut(s) 40, 145, 191, 559, 600, 705
Hpy188III TCNNGA 6 cut(s) 92, 215, 418, 502, 520, 992
Hpy8I GTNNAC 4 cut(s) 322, 512, 1097, 1304
Hpy99I CGWCG 2 cut(s) 1338, 1458
HpyAV CCTTC 5 cut(s) 7, 347, 989, 1400, 1544
HpyCH4III ACNGT 4 cut(s) 368, 931, 1243, 1338
HpyCH4IV ACGT 2 cut(s) 584, 1327
HpyF10VI GCNNNNNNNGC 3 cut(s) 479, 773, 1437
HpyF3I CTNAG 8 cut(s) 78, 597, 887, 1106, 1281, 1441, 1527, 1535
HpySE526I ACGT 2 cut(s) 584, 1327
HspAI GCGC 1 cut(s) 235
Kpn2I TCCGGA 1 cut(s) 417
Kzo9I GATC 3 cut(s) 571, 1408, 1444
LmnI GCTCC 1 cut(s) 494
Lsp1109I GCAGC 3 cut(s) 258, 760, 833
LweI GCATC 1 cut(s) 1306
MaeI CTAG 2 cut(s) 155, 662
MaeII ACGT 2 cut(s) 584, 1327
MaeIII GTNAC 3 cut(s) 406, 748, 998
MalI GATC 3 cut(s) 573, 1410, 1446
MboI GATC 3 cut(s) 571, 1408, 1444
MflI RGATCY 1 cut(s) 1444
MhlI GDGCHC 2 cut(s) 167, 1139
MlsI TGGCCA 3 cut(s) 803, 936, 1361
MluCI AATT 9 cut(s) 5, 184, 391, 399, 669, 725, 982, 1123, 1236
MluNI TGGCCA 3 cut(s) 803, 936, 1361
MlyI GAGTC 1 cut(s) 1525
MmeI TCCRAC 2 cut(s) 582, 1080
Mox20I TGGCCA 3 cut(s) 803, 936, 1361
MroI TCCGGA 1 cut(s) 417
MscI TGGCCA 3 cut(s) 803, 936, 1361
MseI TTAA 5 cut(s) 716, 728, 1205, 1266, 1472
Msp20I TGGCCA 3 cut(s) 803, 936, 1361
MspCI CTTAAG 2 cut(s) 1265, 1471
MspI CCGG 2 cut(s) 418, 847
MspR9I CCNGG 1 cut(s) 255
Mva1269I GAATGC 1 cut(s) 199
MvaI CCWGG 1 cut(s) 255
MvnI CGCG 1 cut(s) 495
MwoI GCNNNNNNNGC 3 cut(s) 479, 773, 1437
NcoI CCATGG 1 cut(s) 345
NdeI CATATG 1 cut(s) 1512
NdeII GATC 3 cut(s) 571, 1408, 1444
NlaIV GGNNCC 1 cut(s) 324
NmuCI GTSAC 2 cut(s) 406, 748
NspI RCATGY 2 cut(s) 799, 1046
PaeR7I CTCGAG 1 cut(s) 1365
PagI TCATGA 1 cut(s) 519
PctI GAATGC 1 cut(s) 199
PfeI GAWTC 4 cut(s) 59, 122, 192, 280
PinAI ACCGGT 1 cut(s) 846
PkrI GCNGC 3 cut(s) 273, 775, 823
PleI GAGTC 1 cut(s) 1525
PpsI GAGTC 1 cut(s) 1525
PshBI ATTAAT 1 cut(s) 728
PsiI TTATAA 1 cut(s) 458
Psp6I CCWGG 1 cut(s) 253
PspGI CCWGG 1 cut(s) 253
PspN4I GGNNCC 1 cut(s) 324
PspPI GGNCC 1 cut(s) 322
PspXI VCTCGAGB 1 cut(s) 1365
PstNI CAGNNNCTG 1 cut(s) 773
PsuI RGATCY 1 cut(s) 1444
RsaI GTAC 4 cut(s) 332, 686, 967, 1073
RsaNI GTAC 4 cut(s) 331, 685, 966, 1072
SaqAI TTAA 5 cut(s) 716, 728, 1205, 1266, 1472
SatI GCNGC 3 cut(s) 272, 774, 822
Sau3AI GATC 3 cut(s) 571, 1408, 1444
Sau96I GGNCC 1 cut(s) 322
ScaI AGTACT 1 cut(s) 1073
SchI GAGTC 1 cut(s) 1525
ScrFI CCNGG 1 cut(s) 255
SduI GDGCHC 2 cut(s) 167, 1139
SfaNI GCATC 1 cut(s) 1306
Sfr274I CTCGAG 1 cut(s) 1365
SinI GGWCC 1 cut(s) 322
SlaI CTCGAG 1 cut(s) 1365
SmlI CTYRAG 4 cut(s) 1250, 1265, 1365, 1471
SmoI CTYRAG 4 cut(s) 1250, 1265, 1365, 1471
SpeI ACTAGT 1 cut(s) 154
Sse9I AATT 9 cut(s) 5, 184, 391, 399, 669, 725, 982, 1123, 1236
SsiI CCGC 3 cut(s) 428, 493, 1570
SspI AATATT 2 cut(s) 1020, 1501
SspMI CTAG 2 cut(s) 155, 662
StyD4I CCNGG 1 cut(s) 253
StyI CCWWGG 1 cut(s) 345
TaaI ACNGT 4 cut(s) 368, 931, 1243, 1338
TaiI ACGT 2 cut(s) 587, 1330
TaqI TCGA 5 cut(s) 93, 283, 1333, 1366, 1453
TasI AATT 9 cut(s) 5, 184, 391, 399, 669, 725, 982, 1123, 1236
TatI WGTACW 3 cut(s) 330, 684, 1071
TfiI GAWTC 4 cut(s) 59, 122, 192, 280
Tru1I TTAA 5 cut(s) 716, 728, 1205, 1266, 1472
Tru9I TTAA 5 cut(s) 716, 728, 1205, 1266, 1472
TscAI CASTG 1 cut(s) 1285
TseFI GTSAC 2 cut(s) 406, 748
TseI GCWGC 3 cut(s) 271, 773, 821
Tsp45I GTSAC 2 cut(s) 406, 748
TspDTI ATGAA 7 cut(s) 280, 392, 536, 1051, 1382, 1392, 1476
TspGWI ACGGA 1 cut(s) 656
TspRI CASTG 1 cut(s) 1285
Vha464I CTTAAG 2 cut(s) 1265, 1471
VpaK11BI GGWCC 1 cut(s) 322
VspI ATTAAT 1 cut(s) 728
XapI RAATTY 3 cut(s) 184, 391, 1123
XceI RCATGY 2 cut(s) 799, 1046
XhoI CTCGAG 1 cut(s) 1365
XspI CTAG 2 cut(s) 155, 662
ZrmI AGTACT 1 cut(s) 1073
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.